AlfalfaGEDB | MsaG041414

M. sativa ssp. caerulea / MsaG041414

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030100 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0060627 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0098657 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041414	XP_003627012.2	80.870	575	39	6	3	507	118	691	0.0	906

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041414	sp\|Q852L0\|HD16N_ORYSJ	62.937	572	140	7	3	505	137	705	0.0	717

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041414	tr\|A0A803LBK0\|A0A803LBK0_CHEQI	68.345	556	123	7	1	505	152	705	0.0	781

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000083	MsaG041414	0.802006	1.155574e-48	1.634897e-46
MsaG000490	MsaG041414	0.811532	1.145613e-50	2.031023e-48
MsaG000712	MsaG041414	0.800721	2.112000e-48	2.902489e-46
MsaG000791	MsaG041414	0.818061	4.157204e-52	8.690780e-50
MsaG001192	MsaG041414	0.801330	1.587490e-48	2.211861e-46
MsaG001323	MsaG041414	0.802972	7.319928e-49	1.058825e-46
MsaG001826	MsaG041414	0.806648	1.259570e-49	1.984923e-47
MsaG002121	MsaG041414	0.818992	2.562652e-52	5.488660e-50
MsaG002681	MsaG041414	0.806793	1.174294e-49	1.856837e-47
MsaG002727	MsaG041414	0.802750	8.131995e-49	1.170282e-46
MsaG003684	MsaG041414	0.801039	1.819953e-48	2.518999e-46
MsaG003815	MsaG041414	0.809669	2.882220e-50	4.882824e-48
MsaG003915	MsaG041414	0.809651	2.909225e-50	4.926414e-48
MsaG005035	MsaG041414	0.806580	1.302129e-49	2.048715e-47
MsaG005138	MsaG041414	0.805203	2.527071e-49	3.849353e-47
MsaG005614	MsaG041414	0.809185	3.657004e-50	6.123418e-48
MsaG006013	MsaG041414	0.804159	4.164210e-49	6.191350e-47
MsaG006049	MsaG041414	0.806113	1.631494e-49	2.538641e-47
MsaG006254	MsaG041414	0.805379	2.322731e-49	3.552519e-47
MsaG006679	MsaG041414	0.818595	3.151878e-52	6.680693e-50
MsaG006681	MsaG041414	0.805000	2.785347e-49	4.222857e-47
MsaG006841	MsaG041414	0.822190	4.762575e-53	1.110092e-50
MsaG007399	MsaG041414	0.812634	6.607151e-51	1.203631e-48
MsaG007700	MsaG041414	0.811100	1.420409e-50	2.491679e-48
MsaG008033	MsaG041414	0.830097	6.394194e-55	1.855721e-52
MsaG008288	MsaG041414	0.812631	6.615834e-51	1.205134e-48
MsaG008430	MsaG041414	0.800649	2.184121e-48	2.996725e-46
MsaG010126	MsaG041414	0.801932	1.196023e-48	1.689362e-46
MsaG010255	MsaG041414	0.804892	2.934157e-49	4.437225e-47
MsaG011257	MsaG041414	0.805403	2.295933e-49	3.513471e-47
MsaG011764	MsaG041414	0.813982	3.352844e-51	6.317516e-49
MsaG011881	MsaG041414	0.808652	4.749810e-50	7.852038e-48
MsaG011996	MsaG041414	0.801613	1.390285e-48	1.949524e-46
MsaG011983	MsaG041414	0.800913	1.929995e-48	2.663740e-46
MsaG012188	MsaG041414	0.805911	1.797809e-49	2.784131e-47
MsaG012339	MsaG041414	0.807671	7.668984e-50	1.238173e-47
MsaG012354	MsaG041414	0.803553	5.556503e-49	8.146243e-47
MsaG012355	MsaG041414	0.802338	9.879778e-49	1.408524e-46
MsaG012610	MsaG041414	0.804216	4.052890e-49	6.033848e-47
MsaG013310	MsaG041414	0.805831	1.868485e-49	2.888159e-47
MsaG013883	MsaG041414	0.802828	7.836707e-49	1.129796e-46
MsaG015976	MsaG041414	0.804104	4.275525e-49	6.348937e-47
MsaG016429	MsaG041414	0.809225	3.585720e-50	6.009690e-48
MsaG017566	MsaG041414	0.801854	1.241006e-48	1.749806e-46
MsaG018355	MsaG041414	0.810402	2.007534e-50	3.462160e-48
MsaG018780	MsaG041414	0.811120	1.406655e-50	2.468715e-48
MsaG019225	MsaG041414	0.803897	4.717467e-49	6.971243e-47
MsaG019533	MsaG041414	0.806390	1.426943e-49	2.235051e-47
MsaG019716	MsaG041414	0.816996	7.205326e-52	1.465563e-49
MsaG020091	MsaG041414	0.805748	1.944725e-49	3.000118e-47
MsaG021085	MsaG041414	0.800733	2.099839e-48	2.886527e-46
MsaG021403	MsaG041414	0.816736	8.237422e-52	1.664238e-49
MsaG021836	MsaG041414	0.808392	5.393723e-50	8.860549e-48
MsaG022048	MsaG041414	0.805148	2.595259e-49	3.948140e-47
MsaG022080	MsaG041414	0.800590	2.244864e-48	3.075947e-46
MsaG022954	MsaG041414	0.803040	7.088758e-49	1.026999e-46
MsaG023249	MsaG041414	0.800544	2.293346e-48	3.139131e-46
MsaG023230	MsaG041414	0.804045	4.396478e-49	6.519709e-47
MsaG025658	MsaG041414	0.806782	1.180823e-49	1.866680e-47
MsaG026753	MsaG041414	0.808235	5.824736e-50	9.532729e-48
MsaG028308	MsaG041414	0.820105	1.432056e-52	3.158522e-50
MsaG028370	MsaG041414	0.810505	1.908313e-50	3.299208e-48
MsaG028328	MsaG041414	0.809376	3.329534e-50	5.600813e-48
MsaG028428	MsaG041414	0.800748	2.085266e-48	2.867466e-46
MsaG028590	MsaG041414	0.809238	3.564005e-50	5.975095e-48
MsaG028601	MsaG041414	0.809739	2.784513e-50	4.725399e-48
MsaG028831	MsaG041414	0.811045	1.459624e-50	2.557049e-48
MsaG029210	MsaG041414	0.801760	1.297385e-48	1.825340e-46
MsaG029311	MsaG041414	0.816432	9.628165e-52	1.930074e-49
MsaG029894	MsaG041414	0.806110	1.633319e-49	2.541338e-47
MsaG030046	MsaG041414	0.808780	4.462385e-50	7.399601e-48
MsaG030879	MsaG041414	0.836895	1.310658e-56	4.647355e-54
MsaG032340	MsaG041414	0.818721	2.951192e-52	6.275893e-50
MsaG032834	MsaG041414	0.818220	3.828985e-52	8.037586e-50
MsaG033872	MsaG041414	0.804751	3.138938e-49	4.731375e-47
MsaG034077	MsaG041414	0.804752	3.136977e-49	4.728551e-47
MsaG034221	MsaG041414	0.812583	6.778582e-51	1.233340e-48
MsaG034231	MsaG041414	0.821256	7.814809e-53	1.776856e-50
MsaG034232	MsaG041414	0.806420	1.406829e-49	2.205122e-47
MsaG034644	MsaG041414	0.800862	1.976980e-48	2.725499e-46
MsaG034653	MsaG041414	0.801896	1.217015e-48	1.717607e-46
MsaG034737	MsaG041414	0.807815	7.149441e-50	1.158317e-47
MsaG034798	MsaG041414	0.806753	1.197540e-49	1.891824e-47
MsaG034957	MsaG041414	0.815304	1.714112e-51	3.339120e-49
MsaG035165	MsaG041414	0.813871	3.545857e-51	6.662803e-49
MsaG036503	MsaG041414	0.800875	1.964585e-48	2.709243e-46
MsaG037106	MsaG041414	0.809838	2.653017e-50	4.512971e-48
MsaG040143	MsaG041414	0.803198	6.575606e-49	9.561625e-47
MsaG041010	MsaG041414	0.812055	8.825342e-51	1.584810e-48
MsaG041066	MsaG041414	0.820094	1.440818e-52	3.176869e-50
MsaG041414	MsaG041510	0.809086	3.840716e-50	6.415781e-48
MsaG041414	MsaG042064	0.800034	2.910147e-48	3.937717e-46
MsaG041414	MsaG042532	0.803186	6.615701e-49	9.617076e-47
MsaG041414	MsaG043285	0.830092	6.413666e-55	1.861065e-52
MsaG041414	MsaG043390	0.800871	1.968116e-48	2.713856e-46
MsaG041414	MsaG043499	0.802005	1.155731e-48	1.635109e-46
MsaG041414	MsaG044691	0.803061	7.016774e-49	1.017070e-46
MsaG041414	MsaG045303	0.802192	1.058218e-48	1.503581e-46
MsaG041414	MsaG046281	0.800314	2.553141e-48	3.476566e-46
MsaG041414	MsaG047016	0.805550	2.139063e-49	3.284589e-47
MsaG041414	MsaG009150	0.804039	4.408674e-49	6.536886e-47
MsaG041414	MsaG019154	0.803108	6.863979e-49	9.960109e-47
MsaG041414	MsaG018951	0.802237	1.036289e-48	1.473900e-46
MsaG041414	MsaG024064	0.807713	7.511479e-50	1.214017e-47
MsaG041414	MsaG033743	0.811751	1.027183e-50	1.830859e-48
MsaG041414	MsaG030695	0.805398	2.301184e-49	3.521113e-47
MsaG041414	MsaG034563	0.817951	4.402611e-52	9.177702e-50
MsaG041414	MsaG033413	0.804230	4.025562e-49	5.995143e-47
MsaG041414	MsaG037392	0.805316	2.393710e-49	3.655742e-47
MsaG041414	MsaG037988	0.805478	2.214578e-49	3.394891e-47
MsaG041414	MsaG042682	0.808125	6.144574e-50	1.002969e-47
MsaG041414	MsaG043061	0.809831	2.661657e-50	4.526961e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041414	MtrunA17_Chr8g0340491	80.870	575	39	6	3	507	118	691	0.0	906
MsaG041414	MtrunA17_Chr3g0140431	65.577	581	118	7	1	507	26	598	0.0	761
MsaG041414	MtrunA17_Chr1g0146301	64.421	579	124	7	3	507	114	684	0.0	751
MsaG041414	MtrunA17_Chr7g0214531	61.324	574	150	7	3	506	27	598	0.0	721
MsaG041414	MtrunA17_Chr7g0253211	62.021	574	145	7	3	506	314	884	0.0	717
MsaG041414	MtrunA17_Chr1g0213321	58.507	576	160	8	1	505	107	674	0.0	688
MsaG041414	MtrunA17_Chr4g0071031	34.602	289	148	7	13	275	9	282	1.44e-45	165
MsaG041414	MtrunA17_Chr1g0166821	35.764	288	144	7	13	274	10	282	2.51e-45	165
MsaG041414	MtrunA17_Chr2g0329981	34.256	289	149	7	13	275	9	282	8.30e-45	164
MsaG041414	MtrunA17_Chr2g0299661	33.562	292	153	7	13	278	9	285	1.62e-44	163
MsaG041414	MtrunA17_Chr1g0187571	35.764	288	144	7	13	274	10	282	2.62e-44	162
MsaG041414	MtrunA17_Chr1g0183631	34.375	288	148	7	13	274	9	281	3.96e-44	161
MsaG041414	MtrunA17_Chr7g0261501	34.722	288	147	6	13	274	9	281	7.83e-44	161
MsaG041414	MtrunA17_Chr8g0342821	34.256	289	148	8	13	275	9	281	5.74e-43	159
MsaG041414	MtrunA17_Chr6g0486611	32.986	288	152	7	13	274	9	281	1.68e-41	154
MsaG041414	MtrunA17_Chr8g0390211	33.681	288	150	7	13	274	9	281	4.16e-41	152
MsaG041414	MtrunA17_Chr1g0167261	34.138	290	148	7	13	274	10	284	6.17e-39	145
MsaG041414	MtrunA17_Chr1g0146151	32.500	280	148	6	13	266	9	273	1.20e-38	145
MsaG041414	MtrunA17_Chr3g0140071	31.944	288	155	7	13	274	9	281	2.64e-37	142

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041414	AT2G25760.1	64.483	580	132	6	1	507	95	673	0.0	769
MsaG041414	AT2G25760.2	63.808	583	134	7	1	507	95	676	0.0	759
MsaG041414	AT3G03940.1	61.257	573	149	7	3	505	130	699	0.0	717
MsaG041414	AT5G18190.2	61.606	573	147	7	3	505	120	689	0.0	716
MsaG041414	AT5G18190.1	61.606	573	147	7	3	505	120	689	0.0	716
MsaG041414	AT3G13670.1	60.449	579	153	7	3	507	127	703	0.0	714
MsaG041414	AT3G03930.1	56.180	267	104	5	252	506	22	287	3.89e-97	295
MsaG041414	AT3G23340.1	35.764	288	144	7	13	274	9	281	8.21e-46	166
MsaG041414	AT1G03930.1	34.722	288	147	7	13	274	9	281	9.92e-46	167
MsaG041414	AT5G44100.1	34.028	288	149	6	13	274	9	281	2.82e-45	166
MsaG041414	AT5G44100.2	34.028	288	149	6	13	274	9	281	2.82e-45	166
MsaG041414	AT4G26100.1	34.256	289	149	6	13	275	9	282	8.07e-45	164
MsaG041414	AT4G14340.2	35.417	288	145	7	13	274	15	287	9.70e-45	161
MsaG041414	AT4G28540.1	35.069	288	146	7	13	274	13	285	1.31e-44	164
MsaG041414	AT5G43320.2	35.069	288	146	6	13	274	9	281	1.97e-44	160
MsaG041414	AT5G43320.3	35.069	288	146	6	13	274	9	281	1.97e-44	160
MsaG041414	AT5G43320.1	35.417	288	145	7	13	274	9	281	2.42e-44	163
MsaG041414	AT2G19470.1	34.256	289	149	7	13	275	9	282	6.73e-44	160
MsaG041414	AT4G14340.1	35.417	288	145	7	13	274	15	287	6.80e-44	161
MsaG041414	AT5G57015.1	34.256	289	149	7	13	275	9	282	9.60e-44	160
MsaG041414	AT1G04440.1	34.028	288	149	7	13	274	9	281	1.61e-42	158
MsaG041414	AT1G72710.1	33.910	289	149	8	13	275	9	281	7.30e-42	156
MsaG041414	AT4G28880.3	31.832	333	181	8	13	314	9	326	1.66e-41	154
MsaG041414	AT4G28880.2	31.832	333	181	8	13	314	9	326	1.66e-41	154
MsaG041414	AT4G28880.1	31.832	333	181	8	13	314	9	326	1.66e-41	154
MsaG041414	AT4G28880.4	31.832	333	181	8	13	314	9	326	1.66e-41	154
MsaG041414	AT4G28860.2	32.639	288	153	6	13	274	9	281	2.61e-41	152
MsaG041414	AT4G28860.1	32.639	288	153	6	13	274	9	281	7.19e-41	152
MsaG041414	AT3G23340.2	36.122	263	134	6	38	274	13	267	7.20e-41	152
MsaG041414	AT4G08800.1	32.384	281	133	8	13	275	9	250	5.26e-34	130

Find 119 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGCTCTTCATCGTACTTTA+AGG	0.153231	8:+6467324	None:intergenic
TGTTCAACAAACTCTTTAAA+AGG	0.210064	8:+6467355	None:intergenic
TGTATGATTGCTGCGCATAC+TGG	0.265771	8:+6465991	None:intergenic
AAACTTGGCAAGGGTGGATT+TGG	0.288608	8:-6469032	MsaT041414.1:CDS
TCGGGCTTGCAGCTACTAAA+TGG	0.289201	8:-6467805	MsaT041414.1:intron
TGTAAGCAGGAGGGATGATT+TGG	0.299076	8:-6467592	MsaT041414.1:CDS
ACCTGAAAAGATCAGGCTTT+TGG	0.301833	8:+6467747	None:intergenic
GCAAGTGTTCATGCACATTT+GGG	0.305910	8:-6467621	MsaT041414.1:CDS
TGCAAGTGTTCATGCACATT+TGG	0.306470	8:-6467622	MsaT041414.1:CDS
TACTATATCACTGCAGTTGC+AGG	0.322061	8:-6466144	MsaT041414.1:CDS
TGGCCACAGCAGGGCCCAAA+TGG	0.322638	8:-6465899	MsaT041414.1:CDS
AAATACATTTCTCTCTTTGA+TGG	0.338871	8:-6467292	MsaT041414.1:CDS
GGGCCTGTGGGCCTTAATAA+TGG	0.345557	8:-6466410	MsaT041414.1:CDS
CACATCGACTGCTGCAACTT+GGG	0.347827	8:-6465673	MsaT041414.1:CDS
GTGATTCATTTCCTTTCAAA+TGG	0.348696	8:-6465962	MsaT041414.1:CDS
TGTCCCTGCATCCATTATTA+AGG	0.355727	8:+6466399	None:intergenic
ACAAAGGATATCAGGTTTGC+AGG	0.362919	8:-6467426	MsaT041414.1:CDS
CATTGAAGCAATATCCATAT+TGG	0.378020	8:-6468237	MsaT041414.1:CDS
CCCACCTTAACATGAGTATT+AGG	0.379485	8:+6465570	None:intergenic
AGAGTGCATCTTCTCCAATA+TGG	0.379754	8:+6468223	None:intergenic
GTAGTTATGTCTCGTGGGGC+AGG	0.381516	8:-6465873	MsaT041414.1:CDS
GCGCAGATTATGGATATTCT+AGG	0.384310	8:-6468435	MsaT041414.1:intron
TCGTACGCTTCCCACTCTTC+TGG	0.386871	8:+6468779	None:intergenic
AGACATAACTACTCCCCATT+TGG	0.388320	8:+6465884	None:intergenic
TAGCAGTGTTGCTTCATCTC+AGG	0.389104	8:-6466431	MsaT041414.1:CDS
TTCTTCGAAGTCGTCTTCCT+TGG	0.394734	8:-6467542	MsaT041414.1:CDS
CAACAAACTCTTTAAAAGGC+TGG	0.396910	8:+6467359	None:intergenic
GGGAGAAAACAAAGGATATC+AGG	0.402180	8:-6467434	MsaT041414.1:CDS
CAATATGGATATTGCTTCAA+TGG	0.411978	8:+6468238	None:intergenic
TGCTTCATCTCAGGGCCTGT+GGG	0.413274	8:-6466422	MsaT041414.1:CDS
TTTCAGTATGTCCACTGAAA+TGG	0.414503	8:-6468273	MsaT041414.1:intron
ATTCACAAGCGGTGGGAGTC+TGG	0.416290	8:-6465706	MsaT041414.1:CDS
GGAAATTTGAATGAGAGAAC+TGG	0.422299	8:-6468978	MsaT041414.1:CDS
AACTGGACATGAAGCTTTAG+AGG	0.426109	8:-6468961	MsaT041414.1:intron
GACATAACTACTCCCCATTT+GGG	0.426694	8:+6465885	None:intergenic
AACAAACTCTTTAAAAGGCT+GGG	0.435906	8:+6467360	None:intergenic
GGCCACAGCAGGGCCCAAAT+GGG	0.439996	8:-6465898	MsaT041414.1:CDS
GCAGTTGCAGGAATTGCAAA+TGG	0.440109	8:-6466132	MsaT041414.1:CDS
TCGTCCTGCAGCTTATTCTC+TGG	0.442854	8:+6465624	None:intergenic
AGCAGTGTTGCTTCATCTCA+GGG	0.451254	8:-6466430	MsaT041414.1:CDS
AATAAATACCTGAAAAGATC+AGG	0.451526	8:+6467740	None:intergenic
AATACATTTCTCTCTTTGAT+GGG	0.456247	8:-6467291	MsaT041414.1:CDS
TTGCTTCATCTCAGGGCCTG+TGG	0.457116	8:-6466423	MsaT041414.1:CDS
TCACATCGACTGCTGCAACT+TGG	0.459764	8:-6465674	MsaT041414.1:CDS
TCCCCATTTGGGCCCTGCTG+TGG	0.465176	8:+6465896	None:intergenic
TACATTGCCTCTATTTGCTA+TGG	0.467256	8:-6465451	MsaT041414.1:CDS
AGTGTATACCTTTGTTTCAT+CGG	0.467387	8:+6466995	None:intergenic
GTAACTTCAATGGCCACAGC+AGG	0.478584	8:-6465909	MsaT041414.1:CDS
GTATGATTGCTGCGCATACT+GGG	0.482995	8:+6465992	None:intergenic
TTAATAATGGATGCAGGGAC+AGG	0.483674	8:-6466397	MsaT041414.1:CDS
TCTTCCTTGGAAAGGATACC+AGG	0.487912	8:-6467529	MsaT041414.1:intron
AAATGGCGGGATAGTACAAC+TGG	0.491011	8:-6467788	MsaT041414.1:CDS
TGATTCATTTCCTTTCAAAT+GGG	0.493009	8:-6465961	MsaT041414.1:CDS
ACACTAATCCACTGGTTCGC+TGG	0.494569	8:+6467034	None:intergenic
GACCTAGCTTGTATGATGAA+TGG	0.497272	8:-6468413	MsaT041414.1:CDS
TCAGGTTTGCAGGAAGAAGA+TGG	0.499604	8:-6467416	MsaT041414.1:CDS
TTGCAGCAGTCGATGTGATT+CGG	0.503800	8:+6465679	None:intergenic
AGGGACAGGTTTCACTTCCC+AGG	0.506444	8:-6466383	MsaT041414.1:CDS
TAGTACAACTGGTTTGCATA+AGG	0.507598	8:-6467777	MsaT041414.1:CDS
TGGGCCTTAATAATGGATGC+AGG	0.508902	8:-6466403	MsaT041414.1:CDS
TTTGAGCACGCAAGTAGTAA+AGG	0.518465	8:-6468818	MsaT041414.1:CDS
CGCTTGTGAATCCCTTCACT+AGG	0.518944	8:+6465718	None:intergenic
TGGAAGTCATGGTGTGCCAC+AGG	0.533084	8:-6468635	MsaT041414.1:intron
GGATTTGGTCAAGTATATGT+TGG	0.538392	8:-6469017	MsaT041414.1:CDS
TCATTAGTAGTAATGTCTAA+AGG	0.539644	8:-6466105	MsaT041414.1:intron
TAGTTATGTCTCGTGGGGCA+GGG	0.540494	8:-6465872	MsaT041414.1:CDS
GTTGGTCGGCGTGTTTCTGA+TGG	0.545961	8:-6468999	MsaT041414.1:CDS
AGTACGTCCATAGCAAATAG+AGG	0.548196	8:+6465444	None:intergenic
GCAAGATGGCTACTATATCA+TGG	0.550126	8:-6468596	MsaT041414.1:intron
CGAAGTCGTCTTCCTTGGAA+AGG	0.554716	8:-6467537	MsaT041414.1:CDS
TTGGTCAAGTATATGTTGGT+CGG	0.554790	8:-6469013	MsaT041414.1:CDS
GGGGAGTAGTTATGTCTCGT+GGG	0.557578	8:-6465878	MsaT041414.1:CDS
CAGTTGCAGGAATTGCAAAT+GGG	0.559687	8:-6466131	MsaT041414.1:CDS
TACTCAGAACAAAAGCAGCT+TGG	0.563974	8:+6465649	None:intergenic
CAATGGACCGCCAGAAGAGT+GGG	0.564362	8:-6468789	MsaT041414.1:CDS
GTGAAGGGATTCACAAGCGG+TGG	0.565396	8:-6465714	MsaT041414.1:CDS
CATTGTAAACACTAATCCAC+TGG	0.568276	8:+6467026	None:intergenic
TGGGGAGTAGTTATGTCTCG+TGG	0.573591	8:-6465879	MsaT041414.1:CDS
CATCCATTATTAAGGCCCAC+AGG	0.574889	8:+6466407	None:intergenic
ACAAACTCTTTAAAAGGCTG+GGG	0.575409	8:+6467361	None:intergenic
GCCACAGCAGGGCCCAAATG+GGG	0.575703	8:-6465897	MsaT041414.1:CDS
TTGCCTTTGTAATGTACCTG+TGG	0.576625	8:+6468619	None:intergenic
ACGAAACTCAAGAAACACTA+AGG	0.576890	8:-6465605	MsaT041414.1:CDS
TATTCCAGAGAATAAGCTGC+AGG	0.578410	8:-6465628	MsaT041414.1:CDS
CAGGTTTGCAGGAAGAAGAT+GGG	0.578830	8:-6467415	MsaT041414.1:CDS
CATTACAAAGGCAAGCAAGA+TGG	0.579097	8:-6468610	MsaT041414.1:CDS
TGGGTTTACCAGCGAACCAG+TGG	0.579970	8:-6467042	MsaT041414.1:CDS
TTCCATTCATCATACAAGCT+AGG	0.582966	8:+6468411	None:intergenic
ATTTGGGGAGAAATGTAAGC+AGG	0.584848	8:-6467605	MsaT041414.1:CDS
ACAATGGACCGCCAGAAGAG+TGG	0.587557	8:-6468790	MsaT041414.1:CDS
GCTTGCAGCTACTAAATGGC+GGG	0.587846	8:-6467801	MsaT041414.1:intron
CTCAGAGACCGATGAAACAA+AGG	0.594714	8:-6467003	MsaT041414.1:intron
GCACATTGACAAAGGAAATG+AGG	0.597073	8:-6466467	MsaT041414.1:CDS
GGGGAGAAATGTAAGCAGGA+GGG	0.597338	8:-6467601	MsaT041414.1:CDS
AACCCAAAAGAAGGTTCGAG+TGG	0.606037	8:-6467062	MsaT041414.1:intron
TATATGGGTAGGTATGTGCA+TGG	0.608359	8:-6467995	MsaT041414.1:intron
TTGGAGAAGATGCACTCTCG+AGG	0.609670	8:-6468218	MsaT041414.1:CDS
AGTAGTAAAGGATGTAACAA+TGG	0.615283	8:-6468806	MsaT041414.1:CDS
GGGCCTTAATAATGGATGCA+GGG	0.621365	8:-6466402	MsaT041414.1:CDS
TGAAGGGATTCACAAGCGGT+GGG	0.622289	8:-6465713	MsaT041414.1:CDS
CTATCACAGCACATTGACAA+AGG	0.627189	8:-6466475	MsaT041414.1:CDS
AGCAAATAGAGGCAATGTAG+AGG	0.629682	8:+6465455	None:intergenic
TGGGGAGAAATGTAAGCAGG+AGG	0.632710	8:-6467602	MsaT041414.1:CDS
TAACTTCAATGGCCACAGCA+GGG	0.633038	8:-6465908	MsaT041414.1:CDS
CAAACCTGGTATCCTTTCCA+AGG	0.635642	8:+6467525	None:intergenic
ATTGACAAAGGAAATGAGGA+TGG	0.636401	8:-6466463	MsaT041414.1:CDS
ACCCAAAAGAAGGTTCGAGT+GGG	0.639354	8:-6467061	MsaT041414.1:intron
TGGAGAAGATGCACTCTCGA+GGG	0.640105	8:-6468217	MsaT041414.1:intron
GGCTTGCAGCTACTAAATGG+CGG	0.641749	8:-6467802	MsaT041414.1:intron
AATACAAGCAACCATTTCAG+TGG	0.645015	8:+6468262	None:intergenic
TACGCTTCCCACTCTTCTGG+CGG	0.651325	8:+6468782	None:intergenic
TGTGCATGGAGATGTGAAGC+CGG	0.653590	8:-6467981	MsaT041414.1:CDS
GGGAGTAGTTATGTCTCGTG+GGG	0.665965	8:-6465877	MsaT041414.1:CDS
GCTTGTGAATCCCTTCACTA+GGG	0.666181	8:+6465719	None:intergenic
GTGCCACAGGTACATTACAA+AGG	0.680188	8:-6468622	MsaT041414.1:CDS
CAAGTGTTCATGCACATTTG+GGG	0.689755	8:-6467620	MsaT041414.1:CDS
AAACAGCAAAGGTTCTCTGG+AGG	0.698314	8:+6467388	None:intergenic
CTAGTGAAGGGATTCACAAG+CGG	0.705426	8:-6465717	MsaT041414.1:CDS
GTGCATGAACACTTGCATAG+CGG	0.721650	8:+6467628	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	6465435	6469806	6465435	ID=MsaG041414
Chr8	mRNA	6465435	6469806	6465435	ID=MsaT041414.1;Parent=MsaG041414
Chr8	exon	6465435	6465494	6465435	ID=MsaT041414.1.exon16;Parent=MsaT041414.1
Chr8	CDS	6465435	6465494	6465435	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6465575	6465751	6465575	ID=MsaT041414.1.exon15;Parent=MsaT041414.1
Chr8	CDS	6465575	6465751	6465575	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6465860	6466017	6465860	ID=MsaT041414.1.exon14;Parent=MsaT041414.1
Chr8	CDS	6465860	6466017	6465860	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6466106	6466181	6466106	ID=MsaT041414.1.exon13;Parent=MsaT041414.1
Chr8	CDS	6466106	6466181	6466106	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6466351	6466525	6466351	ID=MsaT041414.1.exon12;Parent=MsaT041414.1
Chr8	CDS	6466351	6466525	6466351	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6467004	6467074	6467004	ID=MsaT041414.1.exon11;Parent=MsaT041414.1
Chr8	CDS	6467004	6467074	6467004	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6467288	6467455	6467288	ID=MsaT041414.1.exon10;Parent=MsaT041414.1
Chr8	CDS	6467288	6467455	6467288	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6467530	6467659	6467530	ID=MsaT041414.1.exon9;Parent=MsaT041414.1
Chr8	CDS	6467530	6467659	6467530	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6467749	6467815	6467749	ID=MsaT041414.1.exon8;Parent=MsaT041414.1
Chr8	CDS	6467749	6467815	6467749	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6467966	6468006	6467966	ID=MsaT041414.1.exon7;Parent=MsaT041414.1
Chr8	CDS	6467966	6468006	6467966	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6468218	6468289	6468218	ID=MsaT041414.1.exon6;Parent=MsaT041414.1
Chr8	CDS	6468218	6468289	6468218	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6468396	6468451	6468396	ID=MsaT041414.1.exon5;Parent=MsaT041414.1
Chr8	CDS	6468396	6468451	6468396	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6468597	6468651	6468597	ID=MsaT041414.1.exon4;Parent=MsaT041414.1
Chr8	CDS	6468597	6468651	6468597	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6468779	6468852	6468779	ID=MsaT041414.1.exon3;Parent=MsaT041414.1
Chr8	CDS	6468779	6468852	6468779	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6468962	6469093	6468962	ID=MsaT041414.1.exon2;Parent=MsaT041414.1
Chr8	CDS	6468962	6469093	6468962	ID=cds.MsaT041414.1;Parent=MsaT041414.1
Chr8	exon	6469795	6469806	6469795	ID=MsaT041414.1.exon1;Parent=MsaT041414.1
Chr8	CDS	6469795	6469806	6469795	ID=cds.MsaT041414.1;Parent=MsaT041414.1

Gene Sequence

>MsaT041414.1

ATGGTTGACAATGTTCAAGTTAGTACTTCTCCTTTGTATAAAACAGAGAAAAAACTTGGCAAGGGTGGATTTGGTCAAGTATATGTTGGTCGGCGTGTTTCTGATGGAAATTTGAATGAGAGAACTGGACATGAAGCTTTAGAGGTGGCCATAAAATTTGAGCACGCAAGTAGTAAAGGATGTAACAATGGACCGCCAGAAGAGTGGGAAGCGTACGATCATGGTGTGCCACAGGTACATTACAAAGGCAAGCAAGATGGCTACTATATCATGATTATGGATATTCTAGGACCTAGCTTGTATGATGAATGGAATAATAAATCTCACACTATGTCCACTGAAATGGTTGCTTGTATTGCCATTGAAGCAATATCCATATTGGAGAAGATGCACTCTCGAGGGTATGTGCATGGAGATGTGAAGCCGGAAAATTTTCTGCTTGCTACTAAATGGCGGGATAGTACAACTGGTTTGCATAAGGACTATGACCAAAAGCCTGATCTTTTCAGGGGCACGATCCGCTATGCAAGTGTTCATGCACATTTGGGGAGAAATGTAAGCAGGAGGGATGATTTGGAATCTCTTGCTTATACACTGATTTTTCTTCTTCGAAGTCGTCTTCCTTGGAAAGGATACCAGGGAGAAAACAAAGGATATCAGGTTTGCAGGAAGAAGATGGGAACACCTCCAGAGAACCTTTGCTGTTTTTGTCCCCAGCCTTTTAAAGAGTTTGTTGAACATGTAGTGACCTTAAAGTACGATGAAGAGCCCAACTATGCAAAATACATTTCTCTCTTTGATGGGATTAAGGTTCGAGTGGGTTTACCAGCGAACCAGTGGATTAGTGTTTACAATGCTCAGAGACCGATGAAACAAAGATATCACTACAATGTGTCAAGTACAAGACTATCACAGCACATTGACAAAGGAAATGAGGATGGCTTATATATTAGCAGTGTTGCTTCATCTCAGGGCCTGTGGGCCTTAATAATGGATGCAGGGACAGGTTTCACTTCCCAGGTTTATGAATTCTCCCCCCAATTTTTCCATAAGTGGGAAAAAAAAAAATACTATATCACTGCAGTTGCAGGAATTGCAAATGGGAGTTCATTAGTAGTAATGTCTAAAGGCACCCAGTATGCGCAGCAATCATACAAAGTTAGTGATTCATTTCCTTTCAAATGGGTTAGCAAAAAGTGGAAGGAGAAATTTTTTGTAACTTCAATGGCCACAGCAGGGCCCAAATGGGGAGTAGTTATGTCTCGTGGGGCAGGGTTTTCAGAACAGATTTTCTGTACCCTAGTGAAGGGATTCACAAGCGGTGGGAGTCTGGTTTACCGAATCACATCGACTGCTGCAACTTGGGACCAAGCTGCTTTTGTTCTGAGTATTCCAGAGAATAAGCTGCAGGACGAAACTCAAGAAACACTAAGGACTACTACTTTTCCTAATACTCATGTTAAGGAGAAGTGGGCTAAGAACCTCTACATTGCCTCTATTTGCTATGGACGTACTGCTTCTTGA

Protein sequence

>MsaT041414.1

MVDNVQVSTSPLYKTEKKLGKGGFGQVYVGRRVSDGNLNERTGHEALEVAIKFEHASSKGCNNGPPEEWEAYDHGVPQVHYKGKQDGYYIMIMDILGPSLYDEWNNKSHTMSTEMVACIAIEAISILEKMHSRGYVHGDVKPENFLLATKWRDSTTGLHKDYDQKPDLFRGTIRYASVHAHLGRNVSRRDDLESLAYTLIFLLRSRLPWKGYQGENKGYQVCRKKMGTPPENLCCFCPQPFKEFVEHVVTLKYDEEPNYAKYISLFDGIKVRVGLPANQWISVYNAQRPMKQRYHYNVSSTRLSQHIDKGNEDGLYISSVASSQGLWALIMDAGTGFTSQVYEFSPQFFHKWEKKKYYITAVAGIANGSSLVVMSKGTQYAQQSYKVSDSFPFKWVSKKWKEKFFVTSMATAGPKWGVVMSRGAGFSEQIFCTLVKGFTSGGSLVYRITSTAATWDQAAFVLSIPENKLQDETQETLRTTTFPNTHVKEKWAKNLYIASICYGRTAS*