AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041430 MtrunA17_Chr8g0340411 98.678 832 5 1 1 826 1 832 0.0 1702
MsaG041430 MtrunA17_Chr2g0331131 87.575 837 92 5 1 826 1 836 0.0 1529
MsaG041430 MtrunA17_Chr3g0140351 80.263 836 147 6 2 826 7 835 0.0 1409
MsaG041430 MtrunA17_Chr2g0292391 65.707 834 262 11 11 826 9 836 0.0 1131
MsaG041430 MtrunA17_Chr4g0028991 63.799 837 275 12 7 826 21 846 0.0 1086
MsaG041430 MtrunA17_Chr2g0326731 59.595 839 304 13 8 826 16 839 0.0 1019
MsaG041430 MtrunA17_Chr2g0322731 23.759 665 345 24 15 579 103 705 1.93e-25 112
MsaG041430 MtrunA17_Chr8g0350321 22.112 502 292 18 195 661 369 806 2.27e-13 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041430 AT1G52150.1 83.174 838 125 7 1 826 3 836 0.0 1447
MsaG041430 AT1G52150.2 83.075 839 125 8 1 826 3 837 0.0 1442
MsaG041430 AT1G52150.3 82.658 790 121 7 1 778 3 788 0.0 1350
MsaG041430 AT4G32880.1 76.366 842 174 10 1 826 1 833 0.0 1331
MsaG041430 AT2G34710.1 64.362 839 269 15 11 826 21 852 0.0 1082
MsaG041430 AT5G60690.1 62.738 840 279 12 7 826 17 842 0.0 1061
MsaG041430 AT1G30490.1 62.588 850 279 16 1 826 7 841 0.0 1059
MsaG041430 AT5G32670.1 55.319 94 40 1 281 372 37 130 5.89e-26 106
MsaG041430 AT4G21750.2 23.047 512 305 18 185 662 302 758 1.18e-13 75.5
MsaG041430 AT4G21750.2 28.090 178 95 5 4 181 54 198 1.12e-12 72.4
MsaG041430 AT4G21750.1 23.047 512 305 18 185 662 302 758 1.18e-13 75.5
MsaG041430 AT4G21750.1 28.090 178 95 5 4 181 54 198 1.12e-12 72.4
MsaG041430 AT4G21750.3 23.047 512 305 18 185 662 302 758 1.18e-13 75.5
MsaG041430 AT4G21750.3 28.090 178 95 5 4 181 54 198 1.12e-12 72.4
MsaG041430 AT4G21750.4 23.047 512 305 18 185 662 302 758 1.18e-13 75.5
MsaG041430 AT4G21750.4 28.090 178 95 5 4 181 54 198 1.12e-12 72.4
MsaG041430 AT1G05230.3 21.415 509 284 16 189 662 288 715 8.61e-13 72.8
MsaG041430 AT1G05230.8 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.4 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.9 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.10 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.7 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.11 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.2 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.6 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.5 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G05230.1 21.218 509 287 16 189 662 288 717 3.65e-12 70.5
MsaG041430 AT1G73360.1 28.287 251 135 10 15 237 35 268 5.93e-11 66.6
MsaG041430 AT3G61150.1 31.387 137 71 4 7 143 105 218 7.11e-11 66.6

Find 225 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAAGACAACAACTTATT+AGG 0.174771 8:+6768558 MsaT041430.1:CDS
TGGCACCTGCTCGCGACTTT+TGG 0.235961 8:+6770874 MsaT041430.1:CDS
GCTACAAGGGCCAACTTTAA+TGG 0.244448 8:-6772496 None:intergenic
AGCAGAGAAAAGAGTCTTTC+AGG 0.266533 8:+6769330 MsaT041430.1:CDS
TTCATCACCTGACAAGTTAA+TGG 0.282278 8:+6771853 MsaT041430.1:CDS
GTTGGCCCTTGTAGCTTAAC+TGG 0.282373 8:+6772504 MsaT041430.1:CDS
CTCCATCAACAGCTTATTCA+TGG 0.283034 8:-6769381 None:intergenic
CTTCGAATTGAACTTGGTTT+AGG 0.284511 8:-6768542 None:intergenic
CGTATCATCCCTCTTGAATC+TGG 0.289816 8:+6773294 MsaT041430.1:CDS
ATTGGATCTGCAACAGTTAT+AGG 0.295090 8:+6773716 MsaT041430.1:CDS
TGTAGACCTGTTGAATAATC+TGG 0.306622 8:-6774986 None:intergenic
CTGCAGGACTTGCATCCCTT+GGG 0.308926 8:-6770014 None:intergenic
AACCAAGACAAATCAAAGTT+TGG 0.310921 8:+6768606 MsaT041430.1:CDS
AATAAGCACTTAATGGATAA+TGG 0.320902 8:+6768458 MsaT041430.1:CDS
GTGCTTATTACCATCCTTGC+AGG 0.321255 8:-6768444 None:intergenic
GGATGGTAATAAGCACTTAA+TGG 0.322988 8:+6768451 MsaT041430.1:CDS
TCTTGAGATACCTTCATTCC+AGG 0.328882 8:-6770231 None:intergenic
TGACTCACAACTCGTGTCTT+TGG 0.332611 8:-6769950 None:intergenic
TGAGCAAGGGTTTGTGCCTC+AGG 0.333658 8:-6773694 None:intergenic
TCGAAGACAACAACTTATTA+GGG 0.339115 8:+6768559 MsaT041430.1:CDS
AGTGATAATGCTGGAAATTC+TGG 0.341985 8:+6773487 MsaT041430.1:CDS
ATACCTTCATTCCAGGCATT+TGG 0.342067 8:-6770224 None:intergenic
CTCCTTCACAAGGTCTTATC+AGG 0.342292 8:-6771180 None:intergenic
CCTGCAGGACTTGCATCCCT+TGG 0.343323 8:-6770015 None:intergenic
TATAGTGGACGCAGTACTTC+TGG 0.345373 8:-6771339 None:intergenic
TCTCTGGCCATCATTTCTTC+AGG 0.345556 8:-6772718 None:intergenic
GCCCTTGACATTGGCCCTAC+TGG 0.345949 8:+6773451 MsaT041430.1:CDS
AAGTTTGGTTTCAGAATAGA+AGG 0.353326 8:+6768621 MsaT041430.1:CDS
CAAGGCATCAATGCTATTAC+AGG 0.357953 8:+6771934 MsaT041430.1:CDS
CTGTGTAAGCATCCATATTA+TGG 0.372634 8:-6772467 None:intergenic
TTTGCTTTATGTTTGTGAAC+TGG 0.383504 8:+6775264 MsaT041430.1:CDS
CTGAATCTTTCCTTTGGCAA+TGG 0.387020 8:+6771878 MsaT041430.1:CDS
CATCCACATTGTTGATCATA+TGG 0.387385 8:+6771213 MsaT041430.1:CDS
GGATCTCGCGTAGGAAATTA+CGG 0.391373 8:+6772525 MsaT041430.1:CDS
TCAGGTTGCAAGGTGTGAAT+AGG 0.391944 8:+6769348 MsaT041430.1:CDS
AGAATGGGCAGACCATAATA+TGG 0.399984 8:+6772455 MsaT041430.1:CDS
AACCAAACTTTGATTTGTCT+TGG 0.401297 8:-6768608 None:intergenic
ATGGGTCTGAATCTTTCCTT+TGG 0.411939 8:+6771872 MsaT041430.1:CDS
TCCTAAAAGATCGTCCTTTG+TGG 0.414014 8:+6770655 MsaT041430.1:CDS
CCATCATTTCTTCAGGAGAA+TGG 0.415643 8:-6772711 None:intergenic
GCATGTGAACTCAGATTAGA+AGG 0.416254 8:-6773646 None:intergenic
CTAAGTGCCCGCAGAGCTGC+TGG 0.418507 8:-6771644 None:intergenic
GAATTTCTTTCAAAGGCTAC+TGG 0.419741 8:+6770183 MsaT041430.1:CDS
ATACTAACCGGCTCAATCTC+TGG 0.425078 8:-6771667 None:intergenic
ATATAATACTGCGAACATAT+TGG 0.427160 8:-6773591 None:intergenic
CATTGTTGATCATATGGACT+TGG 0.428519 8:+6771219 MsaT041430.1:CDS
TTCAGACCCATTAACTTGTC+AGG 0.430051 8:-6771860 None:intergenic
TCAAGAGCTTCAACCTGCTC+AGG 0.430316 8:-6768500 None:intergenic
CCAAGGGATGCAAGTCCTGC+AGG 0.431428 8:+6770015 MsaT041430.1:CDS
AGGCACAAACCCTTGCTCAT+TGG 0.433054 8:+6773698 MsaT041430.1:CDS
GTTTCTCAGCCCAATGTCAC+TGG 0.437477 8:+6771608 MsaT041430.1:CDS
CTCAATGGATTTACTGATGA+AGG 0.445576 8:+6771779 MsaT041430.1:CDS
TCATCACCTGACAAGTTAAT+GGG 0.446259 8:+6771854 MsaT041430.1:CDS
CATCATTTCTTCAGGAGAAT+GGG 0.448210 8:-6772710 None:intergenic
TATGTTCACGCAGGAACCTT+AGG 0.448822 8:-6772428 None:intergenic
AGCAAGAGCTTGTGGTCTAG+TGG 0.450671 8:+6770522 MsaT041430.1:CDS
TGTGGCACAGGAGGCATGCT+TGG 0.453662 8:-6771124 None:intergenic
TTCACTTTCTCAAACCAAGC+AGG 0.453702 8:+6774864 MsaT041430.1:CDS
TCAGCAAATGAAGCATCAAT+TGG 0.455580 8:-6773243 None:intergenic
CCAGCATGTCAAGCCCTGCT+TGG 0.457309 8:-6774878 None:intergenic
TGCTAAAGTTTCTTCTGCAA+TGG 0.458146 8:-6770160 None:intergenic
CTTCCCCACCCAGTGACATT+GGG 0.458697 8:-6771617 None:intergenic
AAGACGACCAGCAGCTCTGC+GGG 0.458787 8:+6771637 MsaT041430.1:CDS
TATTGAGCTGCTTTATATGC+AGG 0.461758 8:+6770737 MsaT041430.1:CDS
CTTGCATCTGCCCTTGACAT+TGG 0.463238 8:+6773442 MsaT041430.1:CDS
CCAAGCAGGGCTTGACATGC+TGG 0.463621 8:+6774878 MsaT041430.1:CDS
GAGAAAATATTTGATGATAA+TGG 0.466405 8:+6774936 MsaT041430.1:CDS
CGAAATCCAGAGGGTAGAAG+TGG 0.468647 8:-6773273 None:intergenic
TCTTCCCCACCCAGTGACAT+TGG 0.470531 8:-6771618 None:intergenic
CGAGAGAGCTGTTGCTTGGA+AGG 0.471268 8:+6775211 MsaT041430.1:CDS
TGCATTACAAGATATAAGTC+TGG 0.474357 8:+6774914 MsaT041430.1:CDS
GTCTTCTGAGCCAGCACCAT+CGG 0.476703 8:-6771369 None:intergenic
TTTAGCAGAATTTCTTTCAA+AGG 0.476915 8:+6770176 MsaT041430.1:CDS
GCTGGCTCAGAAGACTACTA+TGG 0.477145 8:+6771377 MsaT041430.1:CDS
CAGCCCAATGTCACTGGGTG+GGG 0.478064 8:+6771614 MsaT041430.1:CDS
GGTTTCAATGAGGCACTCAA+TGG 0.478467 8:+6771764 MsaT041430.1:CDS
TGTATTATGTTGCACTCTAA+AGG 0.481143 8:+6774473 MsaT041430.1:CDS
GCAAGATCAAGAGTGCGATT+AGG 0.482451 8:-6773424 None:intergenic
TGCAGGACTTGCATCCCTTG+GGG 0.482836 8:-6770013 None:intergenic
GTTGAGTGGGTCCAAATGCC+TGG 0.484697 8:+6770213 MsaT041430.1:CDS
CTGATATGGACAGCCTGGTC+CGG 0.485461 8:+6770453 MsaT041430.1:intron
GTAGACCTGTTGAATAATCT+GGG 0.486629 8:-6774985 None:intergenic
TGTTGTCTTCGAATTGAACT+TGG 0.487679 8:-6768548 None:intergenic
CAGCATTATCACTTGATGCT+CGG 0.488270 8:-6773477 None:intergenic
ACAGCTGCTGCCATTAAAGT+TGG 0.491933 8:+6772486 MsaT041430.1:CDS
AAATGATAGGTTGCAGAAGC+AGG 0.492355 8:+6769406 MsaT041430.1:CDS
ATTCCAATGGAATCCGGACC+AGG 0.494411 8:-6770466 None:intergenic
GCTATTTCTCATGGTTGCAC+TGG 0.496228 8:+6770493 MsaT041430.1:CDS
GGTTCAATATTAGAAAGAAT+AGG 0.496315 8:-6768587 None:intergenic
TTGCAACAGAAACAATTCTC+TGG 0.496348 8:-6772734 None:intergenic
TCTAATCTGAGTTCACATGC+TGG 0.496973 8:+6773649 MsaT041430.1:CDS
AAAGTGCTAACGCTACCCTT+TGG 0.500197 8:-6773621 None:intergenic
CATACGAGAGAGCTGTTGCT+TGG 0.500411 8:+6775207 MsaT041430.1:CDS
TACTGGAACCGCTGTTGAGT+GGG 0.504776 8:+6770200 MsaT041430.1:CDS
TTGATGCTCGGTTTCCAGTA+GGG 0.507932 8:-6773465 None:intergenic
ATATGAATCACCGATGGTGC+TGG 0.508312 8:+6771359 MsaT041430.1:CDS
AGCCATGAATAAGCTGTTGA+TGG 0.508413 8:+6769379 MsaT041430.1:CDS
TACACTTCTGTTGTAGAAGA+TGG 0.508557 8:+6770906 MsaT041430.1:CDS
ATCAATCACACTCACCCTGC+AGG 0.508922 8:-6770030 None:intergenic
CGAGATCCAGTTAAGCTACA+AGG 0.509433 8:-6772510 None:intergenic
GGAATCGTCGCTATTTCTCA+TGG 0.511365 8:+6770484 MsaT041430.1:CDS
ATCAAGAGTGCGATTAGGAC+TGG 0.512797 8:-6773419 None:intergenic
CTAATCTGAGTTCACATGCT+GGG 0.513411 8:+6773650 MsaT041430.1:CDS
CCATTCTCCTGAAGAAATGA+TGG 0.515783 8:+6772711 MsaT041430.1:CDS
GGTCCAAATGCCTGGAATGA+AGG 0.515906 8:+6770221 MsaT041430.1:CDS
ACCATCGGTGATTCATATAG+TGG 0.517896 8:-6771354 None:intergenic
GCGTGAACATAGATCAGAAT+GGG 0.519247 8:+6772440 MsaT041430.1:CDS
CTTGATGCTCGGTTTCCAGT+AGG 0.520139 8:-6773466 None:intergenic
TCTCACCTCGTGTATGAGAA+TGG 0.523269 8:+6769431 MsaT041430.1:CDS
TTCCAGTAGGGCCAATGTCA+AGG 0.524338 8:-6773453 None:intergenic
TGTTGATCATATGGACTTGG+AGG 0.525009 8:+6771222 MsaT041430.1:CDS
CAGCCTGGTCCGGATTCCAT+TGG 0.526878 8:+6770463 MsaT041430.1:intron
ATGATCAACAATGTGGATGA+TGG 0.527724 8:-6771209 None:intergenic
GATCTCGCGTAGGAAATTAC+GGG 0.527880 8:+6772526 MsaT041430.1:CDS
GCTACTATGGCCTGCAAGGA+TGG 0.529224 8:+6768434 None:intergenic
TGTTGATGGAGGAAAATGAT+AGG 0.529771 8:+6769393 MsaT041430.1:CDS
TGCCTCCAGCCATTCTCCTA+AGG 0.530016 8:+6772412 MsaT041430.1:CDS
GCGACGATTCCAATGGAATC+CGG 0.530819 8:-6770472 None:intergenic
TTGGTAGCTCTGCAGTGGAA+TGG 0.533406 8:+6773179 MsaT041430.1:intron
GCTCAATAGTGTGTGCTAGC+GGG 0.534034 8:-6772563 None:intergenic
GGAATGGATGAGAATGCTAT+TGG 0.534330 8:+6773195 MsaT041430.1:CDS
GATGCCCCACTTCTACCCTC+TGG 0.535941 8:+6773267 MsaT041430.1:CDS
AGCAGGTTGAAGCTCTTGAA+AGG 0.536769 8:+6768504 MsaT041430.1:CDS
TTGGAAGTTATTAAGTTGGA+CGG 0.536877 8:+6772682 MsaT041430.1:CDS
CGAGCATCAAGTGATAATGC+TGG 0.543560 8:+6773478 MsaT041430.1:CDS
GCTCTAACAAAATGTGGCAC+AGG 0.544412 8:-6771136 None:intergenic
CGAGCAGGTGCCAATGTGGT+TGG 0.545131 8:-6770864 None:intergenic
TGAGTTCACATGCTGGGCTG+AGG 0.546668 8:+6773656 MsaT041430.1:CDS
TCAGCCCAATGTCACTGGGT+GGG 0.546882 8:+6771613 MsaT041430.1:CDS
CTGTAATAGCATTGATGCCT+TGG 0.547972 8:-6771933 None:intergenic
AGCAAATATAAGTTCTGCAC+AGG 0.548261 8:-6773220 None:intergenic
GCAGGACTTGCATCCCTTGG+GGG 0.548645 8:-6770012 None:intergenic
ACAGCTCTCTCGTATGAGAC+AGG 0.550158 8:-6775200 None:intergenic
TGGTATTTGTCTCTCAAGCA+TGG 0.550328 8:+6775172 MsaT041430.1:CDS
AACAACCAAAAGTCGCGAGC+AGG 0.550911 8:-6770879 None:intergenic
GAGCAAGGGTTTGTGCCTCA+GGG 0.553358 8:-6773693 None:intergenic
ACGCAGGAACCTTAGGAGAA+TGG 0.554205 8:-6772421 None:intergenic
TATTATATCATCAGTCCAAA+GGG 0.557286 8:+6773606 MsaT041430.1:CDS
CTCAGCCCAATGTCACTGGG+TGG 0.560247 8:+6771612 MsaT041430.1:CDS
AGGAACCTTAGGAGAATGGC+TGG 0.561926 8:-6772417 None:intergenic
AAAGAGTCTTTCAGGTTGCA+AGG 0.562032 8:+6769338 MsaT041430.1:CDS
AGTACTTCTGGAACGCTCCA+TGG 0.562310 8:-6771327 None:intergenic
TCACTTTCTCAAACCAAGCA+GGG 0.563124 8:+6774865 MsaT041430.1:CDS
CTACTTAAATCTAATACTGA+AGG 0.567758 8:+6774408 MsaT041430.1:CDS
CTGATAAGACCTTGTGAAGG+AGG 0.568786 8:+6771181 MsaT041430.1:CDS
TACAGCTACTATGGCCTGCA+AGG 0.569997 8:+6768430 None:intergenic
TGCTCAATAGTGTGTGCTAG+CGG 0.571029 8:-6772564 None:intergenic
GCAAGAGAATGTAGCATGCA+TGG 0.572833 8:+6773564 MsaT041430.1:CDS
GCAAGAGCTTGTGGTCTAGT+GGG 0.580275 8:+6770523 MsaT041430.1:CDS
GAGCAGACATGCTGCCTAGT+GGG 0.581394 8:+6771155 MsaT041430.1:CDS
CTACTGGAACCGCTGTTGAG+TGG 0.586215 8:+6770199 MsaT041430.1:CDS
TAGTGGGACTAGAACCCACT+AGG 0.586368 8:+6770539 MsaT041430.1:CDS
TGATGAAGGATGGACAATGA+TGG 0.587248 8:+6771793 MsaT041430.1:CDS
TTTCTCAGCCCAATGTCACT+GGG 0.588485 8:+6771609 MsaT041430.1:CDS
TCCAGTAGGGCCAATGTCAA+GGG 0.589155 8:-6773452 None:intergenic
GTATTATATCATCAGTCCAA+AGG 0.589604 8:+6773605 MsaT041430.1:CDS
CACTTAGCCAGAGATTGAGC+CGG 0.590279 8:+6771660 MsaT041430.1:CDS
TTGTAGATGCTACTTAGGCG+TGG 0.590476 8:+6774383 MsaT041430.1:intron
ATGGATTTACTGATGAAGGA+TGG 0.595578 8:+6771783 MsaT041430.1:CDS
TGTACGTTATACACCTGAGC+AGG 0.597312 8:+6768487 MsaT041430.1:CDS
TGTTGCAGATCCAATGAGCA+AGG 0.597888 8:-6773708 None:intergenic
GCTTTATGCACCAACCACAT+TGG 0.599013 8:+6770854 MsaT041430.1:intron
AGAAATAGCGACGATTCCAA+TGG 0.599267 8:-6770479 None:intergenic
GGTATTTGTCTCTCAAGCAT+GGG 0.600880 8:+6775173 MsaT041430.1:CDS
TGTTTGCAGGGGTTTCAATG+AGG 0.601250 8:+6771754 MsaT041430.1:intron
ATAAGACCTTGTGAAGGAGG+TGG 0.601925 8:+6771184 MsaT041430.1:CDS
AATAGTTCCACCGCTAGCAG+TGG 0.607297 8:-6770718 None:intergenic
TCTGGACCACTTACCCTAGT+GGG 0.608488 8:-6770553 None:intergenic
CAAGGGATGCAAGTCCTGCA+GGG 0.611498 8:+6770016 MsaT041430.1:CDS
AACACAGCATTACTGACAGA+AGG 0.611683 8:-6771905 None:intergenic
CTAACAAAATGTGGCACAGG+AGG 0.612984 8:-6771133 None:intergenic
AATGTGCTGCCCACTGCTAG+CGG 0.616645 8:+6770708 MsaT041430.1:CDS
ATGTCTGCTCTAACAAAATG+TGG 0.616648 8:-6771142 None:intergenic
GGTGTCGCAGCAAGAGCTTG+TGG 0.617510 8:+6770514 MsaT041430.1:CDS
GATGAAGGATGGACAATGAT+GGG 0.617941 8:+6771794 MsaT041430.1:CDS
ATAGTTCCACCGCTAGCAGT+GGG 0.625406 8:-6770717 None:intergenic
AGCAAGGGTTTGTGCCTCAG+GGG 0.625642 8:-6773692 None:intergenic
GAAATCCAGAGGGTAGAAGT+GGG 0.626534 8:-6773272 None:intergenic
CATGAATAAGCTGTTGATGG+AGG 0.627357 8:+6769382 MsaT041430.1:CDS
AGAAGGAAATCCATTGCCAA+AGG 0.629899 8:-6771888 None:intergenic
GGTCTTATCAGGTACCCACT+AGG 0.630106 8:-6771169 None:intergenic
GTGCTGCCCACTGCTAGCGG+TGG 0.632331 8:+6770711 MsaT041430.1:CDS
TGCGTGAACATAGATCAGAA+TGG 0.632509 8:+6772439 MsaT041430.1:CDS
AGCATCAGAGTGGTGCCACA+AGG 0.633858 8:-6774451 None:intergenic
GTTGCAGATCCAATGAGCAA+GGG 0.634819 8:-6773707 None:intergenic
AGACACGAGTTGTGAGTCAG+TGG 0.635890 8:+6769954 MsaT041430.1:CDS
AGCACACACTATTGAGCATG+AGG 0.637565 8:+6772569 MsaT041430.1:CDS
ATCTGGACCACTTACCCTAG+TGG 0.637854 8:-6770554 None:intergenic
GCTGGGCTGAGGTCACCGCT+AGG 0.640780 8:+6773667 MsaT041430.1:CDS
AGAGCAGACATGCTGCCTAG+TGG 0.643425 8:+6771154 MsaT041430.1:CDS
TGGACAATGATGGGCAATGA+CGG 0.644598 8:+6771803 MsaT041430.1:CDS
GAAGACGACCAGCAGCTCTG+CGG 0.647462 8:+6771636 MsaT041430.1:CDS
CATTGACAATATCAACAGCT+CGG 0.648913 8:-6770689 None:intergenic
GTCGCGAGCAGGTGCCAATG+TGG 0.649892 8:-6770868 None:intergenic
TAATGCTGTGTTATGTGCCA+AGG 0.650005 8:+6771916 MsaT041430.1:CDS
TAGACCTGTTGAATAATCTG+GGG 0.651755 8:-6774984 None:intergenic
ATGGAACCACCTCCTTCACA+AGG 0.652482 8:-6771190 None:intergenic
GAGATCCAGTTAAGCTACAA+GGG 0.653186 8:-6772509 None:intergenic
AATCTGTACTCAAGTCCTTG+TGG 0.653586 8:+6774436 MsaT041430.1:CDS
AGCTTAACTGGATCTCGCGT+AGG 0.653798 8:+6772516 MsaT041430.1:CDS
TCCACTATATGAATCACCGA+TGG 0.655585 8:+6771353 MsaT041430.1:CDS
TGTGAGTCAGTGGTGACTAG+TGG 0.658501 8:+6769964 MsaT041430.1:CDS
CTGAACATAATGTCAAACAA+AGG 0.670183 8:-6775288 None:intergenic
AGGGATGATACGAAATCCAG+AGG 0.676709 8:-6773283 None:intergenic
GTGTGCCTTAGATCTGTGAG+AGG 0.677270 8:+6771077 MsaT041430.1:intron
ATCTCGCGTAGGAAATTACG+GGG 0.678972 8:+6772527 MsaT041430.1:CDS
GGCTCAGAAGACTACTATGG+CGG 0.682781 8:+6771380 MsaT041430.1:CDS
TGGTTTGAGAAAGTGAACAC+TGG 0.684025 8:-6774858 None:intergenic
TACCTGATAAGACCTTGTGA+AGG 0.684274 8:+6771178 MsaT041430.1:CDS
TGTAGAAGATGGCAGCCTAG+TGG 0.684422 8:+6770917 MsaT041430.1:CDS
GGGATGATACGAAATCCAGA+GGG 0.685666 8:-6773282 None:intergenic
AGTCCATATGATCAACAATG+TGG 0.687782 8:-6771216 None:intergenic
CGGCAATCACGAAACCACAA+AGG 0.688045 8:-6770669 None:intergenic
TGTGCCTCAGGGGTTCCTAG+CGG 0.689894 8:-6773682 None:intergenic
AAATCCAGAGGGTAGAAGTG+GGG 0.695513 8:-6773271 None:intergenic
AGTGGGACTAGAACCCACTA+GGG 0.698187 8:+6770540 MsaT041430.1:CDS
ATTCTGATCTATGTTCACGC+AGG 0.699759 8:-6772437 None:intergenic
ACACACTATTGAGCATGAGG+AGG 0.703064 8:+6772572 MsaT041430.1:CDS
AGACCTGTTGAATAATCTGG+GGG 0.704064 8:-6774983 None:intergenic
AACATAATACAGCATCAGAG+TGG 0.704741 8:-6774461 None:intergenic
CATTTGGACCCACTCAACAG+CGG 0.709948 8:-6770208 None:intergenic
AACCTTAGGAGAATGGCTGG+AGG 0.716615 8:-6772414 None:intergenic
AATAGCCATTCTCATACACG+AGG 0.748291 8:-6769436 None:intergenic
GTCACCGCTAGGAACCCCTG+AGG 0.761147 8:+6773678 MsaT041430.1:CDS
TCTCGCGTAGGAAATTACGG+GGG 0.824268 8:+6772528 MsaT041430.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 6768440 6775298 6768440 ID=MsaG041430
Chr8 mRNA 6768440 6775298 6768440 ID=MsaT041430.1;Parent=MsaG041430
Chr8 exon 6768440 6768642 6768440 ID=MsaT041430.1.exon1;Parent=MsaT041430.1
Chr8 CDS 6768440 6768642 6768440 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6769319 6769478 6769319 ID=MsaT041430.1.exon2;Parent=MsaT041430.1
Chr8 CDS 6769319 6769478 6769319 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6769946 6770037 6769946 ID=MsaT041430.1.exon3;Parent=MsaT041430.1
Chr8 CDS 6769946 6770037 6769946 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6770152 6770242 6770152 ID=MsaT041430.1.exon4;Parent=MsaT041430.1
Chr8 CDS 6770152 6770242 6770152 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6770466 6770561 6770466 ID=MsaT041430.1.exon5;Parent=MsaT041430.1
Chr8 CDS 6770466 6770561 6770466 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6770645 6770758 6770645 ID=MsaT041430.1.exon6;Parent=MsaT041430.1
Chr8 CDS 6770645 6770758 6770645 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6770855 6770938 6770855 ID=MsaT041430.1.exon7;Parent=MsaT041430.1
Chr8 CDS 6770855 6770938 6770855 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6771088 6771243 6771088 ID=MsaT041430.1.exon8;Parent=MsaT041430.1
Chr8 CDS 6771088 6771243 6771088 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6771327 6771401 6771327 ID=MsaT041430.1.exon9;Parent=MsaT041430.1
Chr8 CDS 6771327 6771401 6771327 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6771593 6771681 6771593 ID=MsaT041430.1.exon10;Parent=MsaT041430.1
Chr8 CDS 6771593 6771681 6771593 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6771763 6771955 6771763 ID=MsaT041430.1.exon11;Parent=MsaT041430.1
Chr8 CDS 6771763 6771955 6771763 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6772408 6772593 6772408 ID=MsaT041430.1.exon12;Parent=MsaT041430.1
Chr8 CDS 6772408 6772593 6772408 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6772679 6772756 6772679 ID=MsaT041430.1.exon13;Parent=MsaT041430.1
Chr8 CDS 6772679 6772756 6772679 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6773186 6773320 6773186 ID=MsaT041430.1.exon14;Parent=MsaT041430.1
Chr8 CDS 6773186 6773320 6773186 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6773412 6773737 6773412 ID=MsaT041430.1.exon15;Parent=MsaT041430.1
Chr8 CDS 6773412 6773737 6773412 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6774389 6774494 6774389 ID=MsaT041430.1.exon16;Parent=MsaT041430.1
Chr8 CDS 6774389 6774494 6774389 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6774852 6775001 6774852 ID=MsaT041430.1.exon17;Parent=MsaT041430.1
Chr8 CDS 6774852 6775001 6774852 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Chr8 exon 6775152 6775298 6775152 ID=MsaT041430.1.exon18;Parent=MsaT041430.1
Chr8 CDS 6775152 6775298 6775152 ID=cds.MsaT041430.1;Parent=MsaT041430.1
Gene Sequence

>MsaT041430.1

ATGGCCTGCAAGGATGGTAATAAGCACTTAATGGATAATGGAAAGTATGTACGTTATACACCTGAGCAGGTTGAAGCTCTTGAAAGGCTTTATCATGAATGTCCTAAACCAAGTTCAATTCGAAGACAACAACTTATTAGGGAATGTCCTATTCTTTCTAATATTGAACCAAGACAAATCAAAGTTTGGTTTCAGAATAGAAGATGTAGAGAGAAGCAGAGAAAAGAGTCTTTCAGGTTGCAAGGTGTGAATAGGAAGTTGACAGCCATGAATAAGCTGTTGATGGAGGAAAATGATAGGTTGCAGAAGCAGGTGTCTCACCTCGTGTATGAGAATGGCTATTTTCGTCAAAACACCCAAAATGCAACCAAAGACACGAGTTGTGAGTCAGTGGTGACTAGTGGTCAGCACAATATGACTAGTCAACATCCCCCAAGGGATGCAAGTCCTGCAGGGCTTTTGTCCATTGCAGAAGAAACTTTAGCAGAATTTCTTTCAAAGGCTACTGGAACCGCTGTTGAGTGGGTCCAAATGCCTGGAATGAAGCCTGGTCCGGATTCCATTGGAATCGTCGCTATTTCTCATGGTTGCACTGGTGTCGCAGCAAGAGCTTGTGGTCTAGTGGGACTAGAACCCACTAGGGTTGCAGAAATCCTAAAAGATCGTCCTTTGTGGTTTCGTGATTGCCGAGCTGTTGATATTGTCAATGTGCTGCCCACTGCTAGCGGTGGAACTATTGAGCTGCTTTATATGCAGCTTTATGCACCAACCACATTGGCACCTGCTCGCGACTTTTGGTTGTTACGCTACACTTCTGTTGTAGAAGATGGCAGCCTAGTGATCTGTGAGAGGTCTCTTAAAAATACTCAAAATGGTCCAAGCATGCCTCCTGTGCCACATTTTGTTAGAGCAGACATGCTGCCTAGTGGGTACCTGATAAGACCTTGTGAAGGAGGTGGTTCCATCATCCACATTGTTGATCATATGGACTTGGAGCCATGGAGCGTTCCAGAAGTACTGCGTCCACTATATGAATCACCGATGGTGCTGGCTCAGAAGACTACTATGGCGGCAGAGTTCGCAGAAGTTTCTCAGCCCAATGTCACTGGGTGGGGAAGACGACCAGCAGCTCTGCGGGCACTTAGCCAGAGATTGAGCCGGGGTTTCAATGAGGCACTCAATGGATTTACTGATGAAGGATGGACAATGATGGGCAATGACGGTGTTGATGATGTCACTATTCTAGTGAATTCATCACCTGACAAGTTAATGGGTCTGAATCTTTCCTTTGGCAATGGATTTCCTTCTGTCAGTAATGCTGTGTTATGTGCCAAGGCATCAATGCTATTACAGAATGTGCCTCCAGCCATTCTCCTAAGGTTCCTGCGTGAACATAGATCAGAATGGGCAGACCATAATATGGATGCTTACACAGCTGCTGCCATTAAAGTTGGCCCTTGTAGCTTAACTGGATCTCGCGTAGGAAATTACGGGGGTCAAGTGATACTCCCGCTAGCACACACTATTGAGCATGAGGAGTTTTTGGAAGTTATTAAGTTGGACGGAGTTGCCCATTCTCCTGAAGAAATGATGGCCAGAGAATTGTTTCTGTTGCAACTCTGCAGTGGAATGGATGAGAATGCTATTGGCACCTGTGCAGAACTTATATTTGCTCCAATTGATGCTTCATTTGCTGATGATGCCCCACTTCTACCCTCTGGATTTCGTATCATCCCTCTTGAATCTGGAAAAGAATCGTCCAGTCCTAATCGCACTCTTGATCTTGCATCTGCCCTTGACATTGGCCCTACTGGAAACCGAGCATCAAGTGATAATGCTGGAAATTCTGGTTGTGTGAGATCTGTGATGACAATAGCATTTGAATTTGCATATGAAAGTCATATGCAAGAGAATGTAGCATGCATGGCACGCCAATATGTTCGCAGTATTATATCATCAGTCCAAAGGGTAGCGTTAGCACTTTCTCCTTCTAATCTGAGTTCACATGCTGGGCTGAGGTCACCGCTAGGAACCCCTGAGGCACAAACCCTTGCTCATTGGATCTGCAACAGTTATAGATGCTACTTAGGCGTGGAGCTACTTAAATCTAATACTGAAGGAAAAGAATCTGTACTCAAGTCCTTGTGGCACCACTCTGATGCTGTATTATGTTGCACTCTAAAGGCAATGCCAGTGTTCACTTTCTCAAACCAAGCAGGGCTTGACATGCTGGAGACTACGCTAGTTGCATTACAAGATATAAGTCTGGAGAAAATATTTGATGATAATGGTCGAAAAACTCTCTTTTCGGAGTTCCCCCAGATTATTCAACAGGGTTTTGCATGTCTGCAAGGTGGTATTTGTCTCTCAAGCATGGGACGACCTGTCTCATACGAGAGAGCTGTTGCTTGGAAGGTGCTGAATGATGAACAGAATGCTCATTGTATTTGCTTTATGTTTGTGAACTGGTCCTTTGTTTGA
Protein sequence

>MsaT041430.1

MACKDGNKHLMDNGKYVRYTPEQVEALERLYHECPKPSSIRRQQLIRECPILSNIEPRQIKVWFQNRRCREKQRKESFRLQGVNRKLTAMNKLLMEENDRLQKQVSHLVYENGYFRQNTQNATKDTSCESVVTSGQHNMTSQHPPRDASPAGLLSIAEETLAEFLSKATGTAVEWVQMPGMKPGPDSIGIVAISHGCTGVAARACGLVGLEPTRVAEILKDRPLWFRDCRAVDIVNVLPTASGGTIELLYMQLYAPTTLAPARDFWLLRYTSVVEDGSLVICERSLKNTQNGPSMPPVPHFVRADMLPSGYLIRPCEGGGSIIHIVDHMDLEPWSVPEVLRPLYESPMVLAQKTTMAAEFAEVSQPNVTGWGRRPAALRALSQRLSRGFNEALNGFTDEGWTMMGNDGVDDVTILVNSSPDKLMGLNLSFGNGFPSVSNAVLCAKASMLLQNVPPAILLRFLREHRSEWADHNMDAYTAAAIKVGPCSLTGSRVGNYGGQVILPLAHTIEHEEFLEVIKLDGVAHSPEEMMARELFLLQLCSGMDENAIGTCAELIFAPIDASFADDAPLLPSGFRIIPLESGKESSSPNRTLDLASALDIGPTGNRASSDNAGNSGCVRSVMTIAFEFAYESHMQENVACMARQYVRSIISSVQRVALALSPSNLSSHAGLRSPLGTPEAQTLAHWICNSYRCYLGVELLKSNTEGKESVLKSLWHHSDAVLCCTLKAMPVFTFSNQAGLDMLETTLVALQDISLEKIFDDNGRKTLFSEFPQIIQQGFACLQGGICLSSMGRPVSYERAVAWKVLNDEQNAHCICFMFVNWSFV*