AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041435 MtrunA17_Chr8g0340491 92.619 691 47 1 1 687 1 691 0.0 1266
MsaG041435 MtrunA17_Chr3g0140431 79.174 605 115 3 87 687 1 598 0.0 1016
MsaG041435 MtrunA17_Chr1g0146301 71.278 665 167 6 36 687 31 684 0.0 986
MsaG041435 MtrunA17_Chr7g0214531 72.788 599 158 4 91 687 4 599 0.0 944
MsaG041435 MtrunA17_Chr7g0253211 67.073 656 205 6 34 687 239 885 0.0 919
MsaG041435 MtrunA17_Chr1g0213321 65.714 665 196 9 37 686 28 675 0.0 909
MsaG041435 MtrunA17_Chr4g0071031 36.749 283 155 7 124 400 9 273 4.27e-53 189
MsaG041435 MtrunA17_Chr1g0166821 37.456 283 153 6 124 400 10 274 9.34e-53 189
MsaG041435 MtrunA17_Chr2g0329981 36.396 283 156 7 124 400 9 273 9.46e-53 189
MsaG041435 MtrunA17_Chr2g0299661 36.786 280 159 6 124 400 9 273 1.42e-52 188
MsaG041435 MtrunA17_Chr1g0183631 37.143 280 158 6 124 400 9 273 1.64e-51 185
MsaG041435 MtrunA17_Chr7g0261501 37.143 280 158 6 124 400 9 273 3.86e-51 184
MsaG041435 MtrunA17_Chr1g0187571 36.749 283 155 6 124 400 10 274 6.26e-51 184
MsaG041435 MtrunA17_Chr8g0342821 36.462 277 151 8 124 394 9 266 8.51e-51 184
MsaG041435 MtrunA17_Chr8g0390211 35.640 289 160 7 118 400 5 273 2.33e-50 181
MsaG041435 MtrunA17_Chr6g0486611 35.106 282 161 6 124 400 9 273 5.58e-50 180
MsaG041435 MtrunA17_Chr1g0146151 34.276 283 162 6 124 400 9 273 3.69e-48 175
MsaG041435 MtrunA17_Chr1g0167261 36.140 285 156 7 124 400 10 276 1.58e-47 171
MsaG041435 MtrunA17_Chr3g0140071 33.922 283 163 7 124 400 9 273 4.85e-45 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041435 AT2G25760.1 69.420 690 191 4 1 687 1 673 0.0 1022
MsaG041435 AT2G25760.2 68.831 693 193 5 1 687 1 676 0.0 1012
MsaG041435 AT3G13670.1 66.228 684 216 9 13 687 26 703 0.0 934
MsaG041435 AT3G03940.1 62.833 713 227 6 1 687 1 701 0.0 922
MsaG041435 AT5G18190.2 63.845 697 236 5 1 687 1 691 0.0 921
MsaG041435 AT5G18190.1 63.845 697 236 5 1 687 1 691 0.0 921
MsaG041435 AT3G03930.1 64.231 260 89 2 428 686 31 287 1.59e-118 356
MsaG041435 AT3G23340.1 37.456 283 153 6 124 400 9 273 2.14e-53 190
MsaG041435 AT1G03930.1 36.749 283 155 7 124 400 9 273 6.83e-53 189
MsaG041435 AT5G44100.1 36.071 280 161 5 124 400 9 273 9.97e-53 189
MsaG041435 AT5G44100.2 36.071 280 161 5 124 400 9 273 9.97e-53 189
MsaG041435 AT4G26100.1 36.749 283 155 7 124 400 9 273 2.30e-52 187
MsaG041435 AT2G19470.1 36.786 280 159 6 124 400 9 273 8.81e-52 186
MsaG041435 AT4G28540.1 36.749 283 155 7 124 400 13 277 8.91e-52 187
MsaG041435 AT4G14340.2 37.500 280 157 6 124 400 15 279 3.77e-51 182
MsaG041435 AT5G57015.1 36.396 283 156 7 124 400 9 273 4.17e-51 184
MsaG041435 AT4G28860.2 35.357 280 163 5 124 400 9 273 5.90e-51 182
MsaG041435 AT5G43320.1 36.749 283 155 6 124 400 9 273 6.03e-51 184
MsaG041435 AT4G14340.1 37.500 280 157 6 124 400 15 279 6.34e-51 184
MsaG041435 AT4G28880.3 35.689 283 158 6 124 400 9 273 1.72e-50 181
MsaG041435 AT4G28880.2 35.689 283 158 6 124 400 9 273 1.72e-50 181
MsaG041435 AT4G28880.1 35.689 283 158 6 124 400 9 273 1.72e-50 181
MsaG041435 AT4G28880.4 35.689 283 158 6 124 400 9 273 1.72e-50 181
MsaG041435 AT4G28860.1 35.336 283 159 6 124 400 9 273 1.75e-50 181
MsaG041435 AT5G43320.2 36.786 280 159 5 124 400 9 273 2.32e-50 180
MsaG041435 AT5G43320.3 36.786 280 159 5 124 400 9 273 2.32e-50 180
MsaG041435 AT1G72710.1 36.042 283 156 8 124 400 9 272 6.32e-50 181
MsaG041435 AT1G04440.1 35.689 283 158 6 124 400 9 273 9.17e-50 181
MsaG041435 AT3G23340.2 38.372 258 142 6 149 400 13 259 1.70e-48 176
MsaG041435 AT4G08800.1 32.500 280 139 7 124 400 9 241 2.65e-38 144

Find 185 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTCTTCATCATACTTTA+AGG 0.148328 8:-6810536 None:intergenic
TGTTCAACAAACTCTTTAAA+AGG 0.193642 8:-6810505 None:intergenic
AAACTTGGCAAGGGTGGATT+TGG 0.288608 8:+6808786 MsaT041435.1:CDS
GTATTCAGTGGCCTAATGTC+TGG 0.288661 8:-6810607 None:intergenic
TGGAAGCAGGAGGGATGATT+TGG 0.289324 8:+6810268 MsaT041435.1:CDS
GCAAGTGTGCATTCACATTT+GGG 0.302471 8:+6810239 MsaT041435.1:CDS
TCTCTCTTTGATGGGATTGT+TGG 0.307967 8:+6810577 MsaT041435.1:CDS
TGCAAGTGTGCATTCACATT+TGG 0.309144 8:+6810238 MsaT041435.1:CDS
TGTATGATTGCTGCACATAC+TGG 0.319912 8:-6811840 None:intergenic
TGGCCACAGCAGGGCCCAAA+TGG 0.322638 8:+6811932 MsaT041435.1:CDS
TACTATATCACTGCAGTTGC+AGG 0.331120 8:+6811687 MsaT041435.1:CDS
AACAAACTCTTTAAAAGGCT+TGG 0.332669 8:-6810500 None:intergenic
AAATACATTTCTCTCTTTGA+TGG 0.338871 8:+6810568 MsaT041435.1:CDS
GCGTGCAGCTGCGGCGGCGG+CGG 0.340408 8:+6806931 MsaT041435.1:CDS
CGCGCTCCACTACGCATTAC+CGG 0.342109 8:-6806809 None:intergenic
GGGCCTGTGGGCCTTAATAA+TGG 0.345557 8:+6811420 MsaT041435.1:CDS
CACATCGACTGCTGCAACTT+GGG 0.347827 8:+6812153 MsaT041435.1:CDS
AGACATAACTACTCCCCATT+TGG 0.350639 8:-6811947 None:intergenic
TGTCCCTGCATCCATTATTA+AGG 0.355727 8:-6811431 None:intergenic
GCGGCGTGCAGCTGCGGCGG+CGG 0.360716 8:+6806928 MsaT041435.1:CDS
ACAAAGGATATCAGGTTTGC+AGG 0.362919 8:+6810434 MsaT041435.1:CDS
AATCGATACCTGAAAATATC+AGG 0.364931 8:-6810083 None:intergenic
GTGCAGATTATGGATATTCT+AGG 0.366603 8:+6809386 MsaT041435.1:intron
CATTCACATTTGGGGAGAAA+TGG 0.369347 8:+6810248 MsaT041435.1:CDS
TTCTCCGAGGTCGTCTTCCT+TGG 0.374259 8:+6810318 MsaT041435.1:CDS
AAATTGTTCCTGGTAGATCT+TGG 0.375545 8:+6809901 MsaT041435.1:CDS
TTGGTCCAAACCCAGACATT+AGG 0.375997 8:+6810596 MsaT041435.1:CDS
AGAGGAGAAGGAGGAGGAAT+CGG 0.377077 8:+6807006 MsaT041435.1:CDS
CATTGAAGCAATATCCATAT+TGG 0.378020 8:+6809582 MsaT041435.1:CDS
AGTGGCCTAATGTCTGGGTT+TGG 0.378824 8:-6810601 None:intergenic
AGAGTGCATCTTCTCCAATA+TGG 0.379754 8:-6809596 None:intergenic
TCGTACGCTTTCCACTCTTC+TGG 0.382554 8:-6809040 None:intergenic
GTGATTCATTTCCTTTCAAA+TGG 0.385845 8:+6811869 MsaT041435.1:CDS
TAGCAGTGTTGCTTCATCTC+AGG 0.389104 8:+6811399 MsaT041435.1:CDS
GACATAACTACTCCCCATTT+GGG 0.392485 8:-6811946 None:intergenic
AACTGGACCTGAAGCTTTAG+AGG 0.393575 8:+6808857 MsaT041435.1:CDS
ACGGCGGCGTGCAGCTGCGG+CGG 0.397327 8:+6806925 MsaT041435.1:CDS
ACAAACTCTTTAAAAGGCTT+GGG 0.399318 8:-6810499 None:intergenic
GGGAGAAAACAAAGGATATC+AGG 0.402180 8:+6810426 MsaT041435.1:intron
AATGCGTAGTGGAGCGCGCC+GGG 0.403368 8:+6806814 MsaT041435.1:CDS
TTCCTGGTAGATCTTGGATT+GGG 0.405911 8:+6809907 MsaT041435.1:CDS
CAATATGGATATTGCTTCAA+TGG 0.411978 8:-6809581 None:intergenic
TGCTTCATCTCAGGGCCTGT+GGG 0.413274 8:+6811408 MsaT041435.1:CDS
TGGCTTTGTTATTCCTGAAA+AGG 0.414541 8:+6807129 MsaT041435.1:CDS
GGAATCGGGTGGAGCGGAGA+AGG 0.418610 8:+6807021 MsaT041435.1:CDS
ATTCACAAGCGGTGGGAGTC+TGG 0.427074 8:+6812121 MsaT041435.1:CDS
GCACATACCTCTAAAGCTTC+AGG 0.429295 8:-6808864 None:intergenic
GTCGCTTCGTTGGCCGTCCC+CGG 0.433489 8:-6806832 None:intergenic
GGCCACAGCAGGGCCCAAAT+GGG 0.439996 8:+6811933 MsaT041435.1:CDS
GCAGTTGCAGGAATTGCAAA+TGG 0.440109 8:+6811699 MsaT041435.1:CDS
ACCTGAAAATATCAGGCCTT+TGG 0.441501 8:-6810076 None:intergenic
GAGGAGAAGGAGGAGGAATC+GGG 0.442360 8:+6807007 MsaT041435.1:CDS
TCGTCCTGCAGCTTATTCTC+TGG 0.442854 8:-6812202 None:intergenic
TGCGGCGGCGGCGGTTGAAG+AGG 0.445807 8:+6806940 MsaT041435.1:CDS
TTCCAGTATGTCCACTGAAA+TGG 0.446920 8:+6809546 MsaT041435.1:intron
AGCAGTGTTGCTTCATCTCA+GGG 0.451254 8:+6811400 MsaT041435.1:CDS
AGTGTATACCTTTGTTTCAT+CGG 0.454542 8:-6810862 None:intergenic
TTCTGTGACTGTCGCTTCGT+TGG 0.455728 8:-6806842 None:intergenic
AATACATTTCTCTCTTTGAT+GGG 0.456247 8:+6810569 MsaT041435.1:CDS
TTGCTTCATCTCAGGGCCTG+TGG 0.457116 8:+6811407 MsaT041435.1:CDS
GGGGAGTAGTTATGTCTAAA+GGG 0.458523 8:+6811953 MsaT041435.1:CDS
TCACATCGACTGCTGCAACT+TGG 0.459764 8:+6812152 MsaT041435.1:CDS
TCCCCATTTGGGCCCTGCTG+TGG 0.465176 8:-6811935 None:intergenic
AAGACGGCGGCGTGCAGCTG+CGG 0.469475 8:+6806922 MsaT041435.1:CDS
TGGGGAGTAGTTATGTCTAA+AGG 0.471149 8:+6811952 MsaT041435.1:CDS
AGCGGAGAAGGAAGTGATAG+AGG 0.472959 8:+6807033 MsaT041435.1:CDS
GAATACAGAAGGTGCACAAA+AGG 0.476772 8:+6810624 MsaT041435.1:CDS
GTAACTTCAATGGCCACAGC+AGG 0.478584 8:+6811922 MsaT041435.1:CDS
ACACTAATCCATTGGTTCGC+TGG 0.480962 8:-6810823 None:intergenic
TTAATAATGGATGCAGGGAC+AGG 0.483674 8:+6811433 MsaT041435.1:CDS
TATTCAGTGGCCTAATGTCT+GGG 0.488062 8:-6810606 None:intergenic
GTATGATTGCTGCACATACT+GGG 0.491174 8:-6811839 None:intergenic
GTAGTTATGTCTAAAGGGAC+AGG 0.492338 8:+6811958 MsaT041435.1:CDS
CCCACCTTAACATGAGTACT+AGG 0.494087 8:-6812256 None:intergenic
GGTACGTCCGTAGCAAATAG+AGG 0.495558 8:-6812382 None:intergenic
GAATGGCGGGATAGTACAAC+TGG 0.496907 8:+6810035 MsaT041435.1:CDS
GACCTAGCTTGTATGATGAA+TGG 0.497272 8:+6809408 MsaT041435.1:CDS
TCGGGATTGCAGCTACTGAA+TGG 0.497998 8:+6810018 MsaT041435.1:intron
TTCTTATCACAGGTTCAAGT+TGG 0.499270 8:+6808735 MsaT041435.1:intron
TCAGGTTTGCAGGAAGAAGA+TGG 0.499604 8:+6810444 MsaT041435.1:CDS
GTTCCTGGTAGATCTTGGAT+TGG 0.502343 8:+6809906 MsaT041435.1:CDS
GCAACCGCCTCCACTTCAAC+CGG 0.502414 8:-6806890 None:intergenic
TTGCAGCAGTCGATGTGATT+CGG 0.503800 8:-6812147 None:intergenic
GGAGGAGGAATCGGGTGGAG+CGG 0.505040 8:+6807015 MsaT041435.1:CDS
TCTTCCTTGGCAAGGATACC+AGG 0.506247 8:+6810331 MsaT041435.1:CDS
AAGCTTGTTGGTCATAAAAG+AGG 0.506773 8:+6810742 MsaT041435.1:intron
GTCACAGAAGAAGCAGGAAG+AGG 0.507675 8:+6806856 MsaT041435.1:CDS
TGGGCCTTAATAATGGATGC+AGG 0.508902 8:+6811427 MsaT041435.1:CDS
GGAAGTTTGAATGAGAGAAC+TGG 0.512610 8:+6808840 MsaT041435.1:CDS
TACATTGCCTCTATTTGCTA+CGG 0.513465 8:+6812375 MsaT041435.1:CDS
CATTGTAAACACTAATCCAT+TGG 0.515189 8:-6810831 None:intergenic
TTTGAGCACACAAGTAGTAA+AGG 0.516595 8:+6809001 MsaT041435.1:CDS
TGGTGGTCATAGCAATGATA+AGG 0.518333 8:+6807081 MsaT041435.1:CDS
TGGGTTTACCAGCGAACCAA+TGG 0.522242 8:+6810815 MsaT041435.1:CDS
CATCATCTTCTGAAGCCAAT+CGG 0.526443 8:-6810765 None:intergenic
TTCCTCCTCCTTCTCCTCTA+CGG 0.526600 8:-6807002 None:intergenic
AGCACCCTCGGTGGAAGTCA+TGG 0.528225 8:+6809174 MsaT041435.1:intron
GTTGGTCGGCGTGTTTCTGA+TGG 0.531700 8:+6808819 MsaT041435.1:CDS
TGGAAGTCATGGTGTGCCAC+AGG 0.533084 8:+6809185 MsaT041435.1:CDS
GCGACAGTCACAGAAGAAGC+AGG 0.535117 8:+6806850 MsaT041435.1:CDS
AGCAATGATAAGGCTAATGC+TGG 0.535580 8:+6807091 MsaT041435.1:CDS
AGGAATGGATCATGGAGCAG+TGG 0.538311 8:+6811655 MsaT041435.1:intron
GGATTTGGTCAAGTATATGT+TGG 0.538392 8:+6808801 MsaT041435.1:CDS
GATTGCAGCTACTGAATGGC+GGG 0.539376 8:+6810022 MsaT041435.1:intron
TCATTAGTAGTAATGTCTAA+AGG 0.539644 8:+6811726 MsaT041435.1:CDS
TGTGCACCTTCTGTATTCAG+TGG 0.543491 8:-6810619 None:intergenic
GTAGTGGAGCGCGCCGGGGA+CGG 0.547559 8:+6806819 MsaT041435.1:CDS
GGGGAGAAATGGAAGCAGGA+GGG 0.548114 8:+6810259 MsaT041435.1:CDS
GCAAGATGGCTACTATATCA+TGG 0.550126 8:+6809224 MsaT041435.1:CDS
AGTGATAGAGGAGAAACAGA+TGG 0.551136 8:+6807045 MsaT041435.1:CDS
TAATGCGTAGTGGAGCGCGC+CGG 0.554554 8:+6806813 MsaT041435.1:CDS
TTGGTCAAGTATATGTTGGT+CGG 0.554790 8:+6808805 MsaT041435.1:CDS
GGTTGAAGAGGAGCCGGTAG+CGG 0.556045 8:+6806952 MsaT041435.1:CDS
TGTTGTGGCCGTAGAGGAGA+AGG 0.556462 8:+6806994 MsaT041435.1:CDS
CAGTTGCAGGAATTGCAAAT+GGG 0.559687 8:+6811700 MsaT041435.1:CDS
TACTCAGAACAAAAGCAGCT+TGG 0.563974 8:-6812177 None:intergenic
ACAAAGGACCGCCAGAAGAG+TGG 0.565133 8:+6809029 MsaT041435.1:CDS
GTGAAGGGATTCACAAGCGG+TGG 0.565396 8:+6812113 MsaT041435.1:CDS
ATTTGGGGAGAAATGGAAGC+AGG 0.568992 8:+6810255 MsaT041435.1:CDS
GGAATGGATCATGGAGCAGT+GGG 0.573163 8:+6811656 MsaT041435.1:intron
GAAGAAAACTCCGGTTGAAG+TGG 0.574100 8:+6806880 MsaT041435.1:CDS
GCATATTGACAAAGGAAATG+AGG 0.574140 8:+6811363 MsaT041435.1:CDS
CATCCATTATTAAGGCCCAC+AGG 0.574889 8:-6811423 None:intergenic
GCCACAGCAGGGCCCAAATG+GGG 0.575703 8:+6811934 MsaT041435.1:CDS
TGGTCATAAAAGAGGCCGAT+TGG 0.576508 8:+6810750 MsaT041435.1:CDS
TTGCCTTTGTAATGTACCTG+TGG 0.576625 8:-6809201 None:intergenic
ACGAAACTCAAGAAACACTA+AGG 0.576890 8:+6812221 MsaT041435.1:CDS
TATTCCAGAGAATAAGCTGC+AGG 0.578410 8:+6812198 MsaT041435.1:CDS
CAGGTTTGCAGGAAGAAGAT+GGG 0.578830 8:+6810445 MsaT041435.1:CDS
CATTACAAAGGCAAGCAAGA+TGG 0.579097 8:+6809210 MsaT041435.1:CDS
GGATCGGCAGTCTCAAGAAA+CGG 0.579896 8:-6812403 None:intergenic
AACTCCGGTTGAAGTGGAGG+CGG 0.582166 8:+6806886 MsaT041435.1:CDS
TTCCATTCATCATACAAGCT+AGG 0.582966 8:-6809410 None:intergenic
AGCTGTTGTTGTGGCCGTAG+AGG 0.583732 8:+6806988 MsaT041435.1:CDS
CGCTTGTGAATCCCTTCACT+AGG 0.585221 8:-6812109 None:intergenic
GGCCGTAGAGGAGAAGGAGG+AGG 0.585511 8:+6807000 MsaT041435.1:CDS
GATAGAGGAGAAACAGATGG+AGG 0.585778 8:+6807048 MsaT041435.1:CDS
GAAAACTCCGGTTGAAGTGG+AGG 0.586374 8:+6806883 MsaT041435.1:CDS
GGCGGCGGTTGAAGAGGAGC+CGG 0.586448 8:+6806946 MsaT041435.1:CDS
CGAGGTCGTCTTCCTTGGCA+AGG 0.587227 8:+6810323 MsaT041435.1:CDS
ACGTCATCATTTACCGCTAC+CGG 0.589532 8:-6806965 None:intergenic
GAGAAGGAGGAGGAATCGGG+TGG 0.592951 8:+6807010 MsaT041435.1:CDS
TAGTACAACTGGTTTGCAAG+TGG 0.599814 8:+6810046 MsaT041435.1:CDS
CTCGTAGACCGATGAAACAA+AGG 0.600975 8:+6810854 MsaT041435.1:CDS
TTTGCTCAGGGAGAAAACAA+AGG 0.604920 8:+6810418 MsaT041435.1:intron
TATATGGGTAGGTATGTGCA+TGG 0.608359 8:+6809823 MsaT041435.1:intron
TTGGAGAAGATGCACTCTCG+AGG 0.609670 8:+6809601 MsaT041435.1:CDS
ATTGACAAAGGAAATGAGGA+TGG 0.611783 8:+6811367 MsaT041435.1:CDS
ATGCGTAGTGGAGCGCGCCG+GGG 0.613209 8:+6806815 MsaT041435.1:CDS
AGCAAATAGAGGCAATGTAG+AGG 0.614096 8:-6812371 None:intergenic
CACACCATGACTTCCACCGA+GGG 0.617170 8:-6809178 None:intergenic
AGTAGTAAAGGATGTAACAA+AGG 0.619173 8:+6809013 MsaT041435.1:CDS
TACGCTTTCCACTCTTCTGG+CGG 0.620763 8:-6809037 None:intergenic
GGGCCTTAATAATGGATGCA+GGG 0.621365 8:+6811428 MsaT041435.1:CDS
TGAAGGGATTCACAAGCGGT+GGG 0.622289 8:+6812114 MsaT041435.1:CDS
TGTGGCCGTAGAGGAGAAGG+AGG 0.627817 8:+6806997 MsaT041435.1:CDS
GCTGGTGAAGAAGATAGTAA+TGG 0.631286 8:+6807109 MsaT041435.1:CDS
TAACTTCAATGGCCACAGCA+GGG 0.633038 8:+6811923 MsaT041435.1:CDS
TACCCAATCCAAGATCTACC+AGG 0.633951 8:-6809909 None:intergenic
GGAAGAGGAGAAGAAAACTC+CGG 0.636000 8:+6806871 MsaT041435.1:CDS
TGGAGAAGATGCACTCTCGA+GGG 0.640105 8:+6809602 MsaT041435.1:CDS
CGAACCTGGTATCCTTGCCA+AGG 0.641947 8:-6810335 None:intergenic
GGATTGCAGCTACTGAATGG+CGG 0.643976 8:+6810021 MsaT041435.1:intron
TGCAAGTGGACTATGACCAA+AGG 0.644519 8:+6810060 MsaT041435.1:CDS
AATACAAGCAACCATTTCAG+TGG 0.645015 8:-6809557 None:intergenic
ATTAGGCCACTGAATACAGA+AGG 0.651859 8:+6810613 MsaT041435.1:CDS
CTATCACAGCATATTGACAA+AGG 0.652285 8:+6811355 MsaT041435.1:CDS
TGTGCATGGAGATGTGAAGC+CGG 0.653590 8:+6809837 MsaT041435.1:CDS
TAGTTATGTCTAAAGGGACA+GGG 0.654878 8:+6811959 MsaT041435.1:CDS
TGGGGAGAAATGGAAGCAGG+AGG 0.657411 8:+6810258 MsaT041435.1:CDS
CTTGCCAAGGAAGACGACCT+CGG 0.657610 8:-6810322 None:intergenic
ACAATGCCGGTAATGCGTAG+TGG 0.659660 8:+6806803 None:intergenic
ATGGAGGAATTTGATCGTGG+TGG 0.664541 8:+6807064 MsaT041435.1:CDS
TGATGACGTAGCTGTTGTTG+TGG 0.664837 8:+6806979 MsaT041435.1:CDS
CAAGTGTGCATTCACATTTG+GGG 0.674469 8:+6810240 MsaT041435.1:CDS
TTTCTCATCGGGTGTCCCCG+AGG 0.676645 8:-6809878 None:intergenic
GTGCCACAGGTACATTACAA+AGG 0.680188 8:+6809198 MsaT041435.1:CDS
AAACAGCATAGGTTCTCTGG+AGG 0.692660 8:-6810472 None:intergenic
CAGATGGAGGAATTTGATCG+TGG 0.693928 8:+6807061 MsaT041435.1:CDS
CTAGTGAAGGGATTCACAAG+CGG 0.700422 8:+6812110 MsaT041435.1:CDS
TATTTGTGCAGCACCCTCGG+TGG 0.705685 8:+6809165 MsaT041435.1:intron
GTGAATGCACACTTGCATAG+CGG 0.721588 8:-6810232 None:intergenic
CGGTTGCGACGAGAACAAGA+CGG 0.733543 8:+6806906 MsaT041435.1:CDS
GCACACCATGACTTCCACCG+AGG 0.742653 8:-6809179 None:intergenic
TTGCGACGAGAACAAGACGG+CGG 0.766461 8:+6806909 MsaT041435.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 6806806 6812413 6806806 ID=MsaG041435
Chr8 mRNA 6806806 6812413 6806806 ID=MsaT041435.1;Parent=MsaG041435
Chr8 exon 6806806 6807150 6806806 ID=MsaT041435.1.exon1;Parent=MsaT041435.1
Chr8 CDS 6806806 6807150 6806806 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6808747 6808878 6808747 ID=MsaT041435.1.exon2;Parent=MsaT041435.1
Chr8 CDS 6808747 6808878 6808747 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6808989 6809062 6808989 ID=MsaT041435.1.exon3;Parent=MsaT041435.1
Chr8 CDS 6808989 6809062 6808989 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6809176 6809245 6809176 ID=MsaT041435.1.exon4;Parent=MsaT041435.1
Chr8 CDS 6809176 6809245 6809176 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6809392 6809447 6809392 ID=MsaT041435.1.exon5;Parent=MsaT041435.1
Chr8 CDS 6809392 6809447 6809392 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6809552 6809623 6809552 ID=MsaT041435.1.exon6;Parent=MsaT041435.1
Chr8 CDS 6809552 6809623 6809552 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6809834 6809928 6809834 ID=MsaT041435.1.exon7;Parent=MsaT041435.1
Chr8 CDS 6809834 6809928 6809834 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6810030 6810096 6810030 ID=MsaT041435.1.exon8;Parent=MsaT041435.1
Chr8 CDS 6810030 6810096 6810030 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6810223 6810352 6810223 ID=MsaT041435.1.exon9;Parent=MsaT041435.1
Chr8 CDS 6810223 6810352 6810223 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6810427 6810645 6810427 ID=MsaT041435.1.exon10;Parent=MsaT041435.1
Chr8 CDS 6810427 6810645 6810427 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6810745 6810875 6810745 ID=MsaT041435.1.exon11;Parent=MsaT041435.1
Chr8 CDS 6810745 6810875 6810745 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6811327 6811501 6811327 ID=MsaT041435.1.exon12;Parent=MsaT041435.1
Chr8 CDS 6811327 6811501 6811327 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6811657 6811747 6811657 ID=MsaT041435.1.exon13;Parent=MsaT041435.1
Chr8 CDS 6811657 6811747 6811657 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6811836 6811993 6811836 ID=MsaT041435.1.exon14;Parent=MsaT041435.1
Chr8 CDS 6811836 6811993 6811836 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6812085 6812273 6812085 ID=MsaT041435.1.exon15;Parent=MsaT041435.1
Chr8 CDS 6812085 6812273 6812085 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Chr8 exon 6812354 6812413 6812354 ID=MsaT041435.1.exon16;Parent=MsaT041435.1
Chr8 CDS 6812354 6812413 6812354 ID=cds.MsaT041435.1;Parent=MsaT041435.1
Gene Sequence

>MsaT041435.1

ATGCCGGTAATGCGTAGTGGAGCGCGCCGGGGACGGCCAACGAAGCGACAGTCACAGAAGAAGCAGGAAGAGGAGAAGAAAACTCCGGTTGAAGTGGAGGCGGTTGCGACGAGAACAAGACGGCGGCGTGCAGCTGCGGCGGCGGCGGTTGAAGAGGAGCCGGTAGCGGTAAATGATGACGTAGCTGTTGTTGTGGCCGTAGAGGAGAAGGAGGAGGAATCGGGTGGAGCGGAGAAGGAAGTGATAGAGGAGAAACAGATGGAGGAATTTGATCGTGGTGGTCATAGCAATGATAAGGCTAATGCTGGTGAAGAAGATAGTAATGGCTTTGTTATTCCTGAAAAGGTTCAAGTTGGTGCTTCTCCTTTGTATAAAACAGAGAAAAAACTTGGCAAGGGTGGATTTGGTCAAGTATATGTTGGTCGGCGTGTTTCTGATGGAAGTTTGAATGAGAGAACTGGACCTGAAGCTTTAGAGGTGGCCATAAAATTTGAGCACACAAGTAGTAAAGGATGTAACAAAGGACCGCCAGAAGAGTGGAAAGCGTACGACACCCTCGGTGGAAGTCATGGTGTGCCACAGGTACATTACAAAGGCAAGCAAGATGGCTACTATATCATGATTATGGATATTCTAGGACCTAGCTTGTATGATGAATGGAATAATAAATCTCACACTATGTCCACTGAAATGGTTGCTTGTATTGCCATTGAAGCAATATCCATATTGGAGAAGATGCACTCTCGAGGGTATGTGCATGGAGATGTGAAGCCGGAAAATTTTCTGCTTGGCGCCTCGGGGACACCCGATGAGAAAAAATTGTTCCTGGTAGATCTTGGATTGGCTACTGAATGGCGGGATAGTACAACTGGTTTGCAAGTGGACTATGACCAAAGGCCTGATATTTTCAGGGGCACAGTCCGCTATGCAAGTGTGCATTCACATTTGGGGAGAAATGGAAGCAGGAGGGATGATTTGGAATCTCTTGCATATACACTGATTTTTCTTCTCCGAGGTCGTCTTCCTTGGCAAGGATACCAGGGAGAAAACAAAGGATATCAGGTTTGCAGGAAGAAGATGGGAACGCCTCCAGAGAACCTATGCTGTTTTTGTCCCAAGCCTTTTAAAGAGTTTGTTGAACATGTAGTGACCTTAAAGTATGATGAAGAGCCCAACTATGCAAAATACATTTCTCTCTTTGATGGGATTGTTGGTCCAAACCCAGACATTAGGCCACTGAATACAGAAGGTGCACAAAAGCTTGTTGGTCATAAAAGAGGCCGATTGGCTTCAGAAGATGATGATGAACAGCCCAAAAAGAAGGTTCGAGTGGGTTTACCAGCGAACCAATGGATTAGTGTTTACAATGCTCGTAGACCGATGAAACAAAGATATCACTACAATGTGTCAAGTACAAGACTATCACAGCATATTGACAAAGGAAATGAGGATGGCTTATATATTAGCAGTGTTGCTTCATCTCAGGGCCTGTGGGCCTTAATAATGGATGCAGGGACAGGTTTCACTTCCCAAGTTTATGAATTCTCCCCCCAATTTTTCCATAAGGAATGGATCATGGAGCAGTGGGAAAAAAATTACTATATCACTGCAGTTGCAGGAATTGCAAATGGGAGTTCATTAGTAGTAATGTCTAAAGGCACCCAGTATGTGCAGCAATCATACAAAGTTAGTGATTCATTTCCTTTCAAATGGATTAGCAAAAAGTGGAGGGAGAAGTTTTTTGTAACTTCAATGGCCACAGCAGGGCCCAAATGGGGAGTAGTTATGTCTAAAGGGACAGGGTTTTCAAAACAGGCGGTCGAATTAGATTTTCTGTATCCTAGTGAAGGGATTCACAAGCGGTGGGAGTCTGGTTACCGAATCACATCGACTGCTGCAACTTGGGACCAAGCTGCTTTTGTTCTGAGTATTCCAGAGAATAAGCTGCAGGACGAAACTCAAGAAACACTAAGGACTACTACTTTTCCTAGTACTCATGTTAAGGAGAAGTGGGCTAAGAACCTCTACATTGCCTCTATTTGCTACGGACGTACCGTTTCTTGA
Protein sequence

>MsaT041435.1

MPVMRSGARRGRPTKRQSQKKQEEEKKTPVEVEAVATRTRRRRAAAAAAVEEEPVAVNDDVAVVVAVEEKEEESGGAEKEVIEEKQMEEFDRGGHSNDKANAGEEDSNGFVIPEKVQVGASPLYKTEKKLGKGGFGQVYVGRRVSDGSLNERTGPEALEVAIKFEHTSSKGCNKGPPEEWKAYDTLGGSHGVPQVHYKGKQDGYYIMIMDILGPSLYDEWNNKSHTMSTEMVACIAIEAISILEKMHSRGYVHGDVKPENFLLGASGTPDEKKLFLVDLGLATEWRDSTTGLQVDYDQRPDIFRGTVRYASVHSHLGRNGSRRDDLESLAYTLIFLLRGRLPWQGYQGENKGYQVCRKKMGTPPENLCCFCPKPFKEFVEHVVTLKYDEEPNYAKYISLFDGIVGPNPDIRPLNTEGAQKLVGHKRGRLASEDDDEQPKKKVRVGLPANQWISVYNARRPMKQRYHYNVSSTRLSQHIDKGNEDGLYISSVASSQGLWALIMDAGTGFTSQVYEFSPQFFHKEWIMEQWEKNYYITAVAGIANGSSLVVMSKGTQYVQQSYKVSDSFPFKWISKKWREKFFVTSMATAGPKWGVVMSKGTGFSKQAVELDFLYPSEGIHKRWESGYRITSTAATWDQAAFVLSIPENKLQDETQETLRTTTFPSTHVKEKWAKNLYIASICYGRTVS*