AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041554

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041554 MtrunA17_Chr8g0341671 95.694 209 6 1 1 206 10 218 4.97e-126 354
MsaG041554 MtrunA17_Chr8g0374111 56.667 120 50 2 6 123 24 143 1.88e-36 127
MsaG041554 MtrunA17_Chr2g0328021 51.145 131 57 3 6 134 57 182 1.02e-35 127
MsaG041554 MtrunA17_Chr4g0031411 61.628 86 32 1 6 91 70 154 7.14e-32 118
MsaG041554 MtrunA17_Chr8g0374251 39.062 192 93 5 8 183 65 248 1.93e-31 117
MsaG041554 MtrunA17_Chr7g0251181 57.812 128 22 3 1 128 10 105 1.31e-30 109
MsaG041554 MtrunA17_Chr7g0223311 75.385 65 16 0 12 76 78 142 2.75e-30 114
MsaG041554 MtrunA17_Chr2g0308011 50.000 122 47 2 11 126 37 150 3.61e-30 110
MsaG041554 MtrunA17_Chr4g0047001 65.000 80 26 1 12 89 56 135 5.58e-30 114
MsaG041554 MtrunA17_Chr4g0030051 77.049 61 14 0 2 62 32 92 1.09e-29 111
MsaG041554 MtrunA17_Chr7g0237921 60.638 94 35 2 4 95 68 161 1.65e-29 112
MsaG041554 MtrunA17_Chr4g0010151 52.679 112 38 3 4 102 74 183 2.67e-29 111
MsaG041554 MtrunA17_Chr4g0061761 84.906 53 8 0 9 61 38 90 2.90e-29 110
MsaG041554 MtrunA17_Chr8g0346551 54.545 88 40 0 8 95 21 108 5.96e-29 108
MsaG041554 MtrunA17_Chr5g0411951 75.000 56 14 0 6 61 38 93 1.46e-28 109
MsaG041554 MtrunA17_Chr8g0367161 62.821 78 25 1 11 88 32 105 1.74e-28 108
MsaG041554 MtrunA17_Chr3g0127201 71.875 64 16 2 6 68 21 83 1.09e-27 105
MsaG041554 MtrunA17_Chr1g0188851 70.492 61 18 0 2 62 34 94 1.40e-27 105
MsaG041554 MtrunA17_Chr2g0282511 87.755 49 6 0 11 59 45 93 1.93e-27 105
MsaG041554 MtrunA17_Chr3g0117601 76.786 56 13 0 6 61 56 111 7.31e-27 104
MsaG041554 MtrunA17_Chr2g0326001 70.492 61 18 0 2 62 31 91 2.06e-26 102
MsaG041554 MtrunA17_Chr3g0126201 84.000 50 8 0 13 62 53 102 2.34e-26 103
MsaG041554 MtrunA17_Chr6g0453321 60.870 69 27 0 11 79 136 204 2.87e-26 105
MsaG041554 MtrunA17_Chr4g0047761 77.358 53 12 0 9 61 70 122 7.56e-26 101
MsaG041554 MtrunA17_Chr2g0282441 78.431 51 11 0 12 62 26 76 1.48e-25 101
MsaG041554 MtrunA17_Chr2g0283811 67.857 56 18 0 8 63 44 99 1.92e-25 97.1
MsaG041554 MtrunA17_Chr1g0177251 82.000 50 9 0 13 62 14 63 2.76e-25 99.8
MsaG041554 MtrunA17_Chr7g0217581 62.857 70 25 1 11 80 232 300 4.11e-25 102
MsaG041554 MtrunA17_Chr2g0282051 78.000 50 11 0 9 58 44 93 5.34e-25 98.6
MsaG041554 MtrunA17_Chr7g0254161 35.519 183 95 4 11 173 101 280 7.37e-25 100
MsaG041554 MtrunA17_Chr3g0092111 38.514 148 85 1 11 152 136 283 1.32e-24 100
MsaG041554 MtrunA17_Chr5g0417601 64.615 65 23 0 11 75 140 204 2.73e-24 98.6
MsaG041554 MtrunA17_Chr6g0460731 47.561 82 43 0 11 92 96 177 3.91e-24 97.8
MsaG041554 MtrunA17_Chr8g0355261 69.231 52 16 0 11 62 118 169 4.25e-24 98.6
MsaG041554 MtrunA17_Chr5g0417571 65.000 60 20 1 3 62 131 189 7.05e-24 96.7
MsaG041554 MtrunA17_Chr4g0042481 69.231 52 16 0 11 62 122 173 1.64e-23 97.1
MsaG041554 MtrunA17_Chr6g0460721 45.977 87 47 0 11 97 89 175 3.30e-23 94.7
MsaG041554 MtrunA17_Chr5g0417551 53.409 88 35 1 11 92 127 214 6.30e-23 94.7
MsaG041554 MtrunA17_Chr5g0417561 56.471 85 29 2 11 87 119 203 2.90e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041554 AT1G51700.1 57.664 137 51 2 2 133 24 158 1.03e-45 150
MsaG041554 AT3G50410.1 52.500 120 38 3 1 101 14 133 7.98e-33 119
MsaG041554 AT3G21270.1 52.113 142 58 4 11 142 29 170 9.36e-33 117
MsaG041554 AT5G60850.1 64.368 87 27 1 7 93 47 129 1.05e-32 120
MsaG041554 AT5G66940.1 70.423 71 21 0 7 77 28 98 2.18e-32 117
MsaG041554 AT5G60200.1 59.302 86 33 1 6 89 50 135 1.61e-30 113
MsaG041554 AT3G45610.1 68.831 77 20 3 2 76 32 106 3.07e-30 112
MsaG041554 AT1G07640.1 73.438 64 17 0 6 69 16 79 3.67e-30 112
MsaG041554 AT1G07640.2 73.438 64 17 0 6 69 72 135 6.71e-30 113
MsaG041554 AT5G65590.1 69.014 71 21 1 7 76 37 107 7.50e-30 112
MsaG041554 AT1G07640.3 73.438 64 17 0 6 69 80 143 8.80e-30 112
MsaG041554 AT4G38000.1 58.242 91 33 1 2 92 32 117 3.36e-29 109
MsaG041554 AT2G28810.2 75.439 57 14 0 6 62 72 128 4.54e-29 110
MsaG041554 AT2G28810.1 75.439 57 14 0 6 62 89 145 6.55e-29 110
MsaG041554 AT3G52440.1 55.208 96 39 1 13 104 27 122 2.04e-28 107
MsaG041554 AT3G52440.2 55.208 96 39 1 13 104 46 141 2.69e-28 107
MsaG041554 AT1G21340.1 53.061 98 43 1 7 101 31 128 3.48e-28 107
MsaG041554 AT2G37590.1 75.862 58 13 1 5 62 84 140 4.76e-28 108
MsaG041554 AT3G55370.1 40.000 145 66 3 11 135 76 219 6.05e-28 107
MsaG041554 AT3G61850.2 63.889 72 19 1 8 79 59 123 6.45e-28 107
MsaG041554 AT3G61850.3 63.889 72 19 1 8 79 59 123 6.45e-28 107
MsaG041554 AT3G55370.3 39.610 154 71 4 2 135 113 264 7.73e-28 108
MsaG041554 AT3G55370.2 40.000 145 66 3 11 135 76 219 7.96e-28 108
MsaG041554 AT3G61850.1 63.889 72 19 1 8 79 71 135 8.09e-28 107
MsaG041554 AT3G61850.5 63.889 72 19 1 8 79 59 123 8.91e-28 107
MsaG041554 AT3G61850.4 63.889 72 19 1 8 79 71 135 1.14e-27 107
MsaG041554 AT1G28310.1 84.615 52 8 0 11 62 27 78 1.42e-27 106
MsaG041554 AT1G28310.2 84.615 52 8 0 11 62 51 102 2.05e-27 106
MsaG041554 AT2G28510.1 75.806 62 14 1 9 70 46 106 4.42e-27 104
MsaG041554 AT4G24060.1 74.138 58 14 1 6 62 47 104 1.60e-26 104
MsaG041554 AT5G62940.1 76.786 56 13 0 6 61 68 123 2.08e-26 104
MsaG041554 AT5G02460.1 71.667 60 16 1 4 62 87 146 7.68e-26 103
MsaG041554 AT4G00940.1 72.414 58 16 0 8 65 65 122 1.69e-25 100
MsaG041554 AT4G00940.3 72.414 58 16 0 8 65 65 122 1.69e-25 100
MsaG041554 AT4G00940.2 72.414 58 16 0 8 65 65 122 1.69e-25 100
MsaG041554 AT2G46590.1 76.471 51 12 0 8 58 65 115 2.30e-25 101
MsaG041554 AT2G46590.2 76.471 51 12 0 8 58 77 127 2.59e-25 101
MsaG041554 AT1G29160.1 73.077 52 14 0 11 62 62 113 3.44e-25 97.1
MsaG041554 AT1G64620.1 75.000 56 13 1 6 60 43 98 3.79e-25 100
MsaG041554 AT2G34140.1 73.077 52 14 0 11 62 58 109 4.13e-25 96.7
MsaG041554 AT4G21050.1 80.000 50 10 0 13 62 26 75 4.43e-25 97.8
MsaG041554 AT1G69570.1 73.077 52 14 0 11 62 132 183 6.66e-25 100
MsaG041554 AT1G47655.1 73.077 52 14 0 9 60 27 78 8.35e-25 97.1
MsaG041554 AT3G47500.1 61.644 73 27 1 11 83 110 181 9.29e-25 100
MsaG041554 AT5G39660.3 59.420 69 28 0 11 79 138 206 9.66e-24 98.2
MsaG041554 AT5G39660.1 59.420 69 28 0 11 79 138 206 9.66e-24 98.2
MsaG041554 AT5G39660.2 59.420 69 28 0 11 79 138 206 9.66e-24 98.2
MsaG041554 AT1G26790.1 69.231 52 16 0 11 62 111 162 2.43e-23 96.3
MsaG041554 AT5G62430.1 69.231 52 16 0 11 62 54 105 6.10e-23 94.0
MsaG041554 AT4G21040.1 68.519 54 17 0 13 66 27 80 8.84e-23 92.0
MsaG041554 AT4G21080.1 51.724 87 36 2 13 99 27 107 2.35e-22 91.3
MsaG041554 AT4G21030.1 62.000 50 19 0 13 62 23 72 4.99e-14 68.2

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACTAAAGGTGGCGCTTTA+AGG 0.181398 8:-8456053 MsaT041554.1:CDS
TGTTCTTGTTCTGGAAAATT+TGG 0.207790 8:+8456180 None:intergenic
AAATGGGTCGGGTTTGAAAA+TGG 0.208231 8:-8455773 MsaT041554.1:CDS
ATCTTTCTTGGTTTGCAAAA+TGG 0.257606 8:-8455664 MsaT041554.1:CDS
CTTGCATCAACTGAGCGATT+TGG 0.269361 8:-8455820 MsaT041554.1:CDS
TTCTGGAAAATTTGGTTTCA+TGG 0.271012 8:+8456188 None:intergenic
CATTTGAGTTGTTCTTGTTC+TGG 0.291270 8:+8456171 None:intergenic
CCCGGTTGTGAATACGGATT+TGG 0.293449 8:-8455722 MsaT041554.1:CDS
GGGGAATCAGAGATCTTTCT+TGG 0.299700 8:-8455676 MsaT041554.1:CDS
CACCCGGGTCAACATGAATC+TGG 0.303329 8:+8455878 None:intergenic
TTGCATCAACTGAGCGATTT+GGG 0.303955 8:-8455819 MsaT041554.1:CDS
GACCCGGGTGAGAGGAATTT+TGG 0.316050 8:-8455865 MsaT041554.1:CDS
TTGAATTCAGACCTTCAAAA+AGG 0.316361 8:+8455794 None:intergenic
CATAACCCGGGTGGTAGAAA+TGG 0.353048 8:-8455697 MsaT041554.1:CDS
TTTGAAGGTCTGAATTCAAA+TGG 0.368494 8:-8455790 MsaT041554.1:CDS
CCCAAATCCGTATTCACAAC+CGG 0.400363 8:+8455721 None:intergenic
ATAACCCGGGTGGTAGAAAT+GGG 0.416940 8:-8455696 MsaT041554.1:CDS
AATGGGTCGGGTTTGAAAAT+GGG 0.442550 8:-8455772 MsaT041554.1:CDS
CTGTGACCCGGTTGTGAATA+CGG 0.450073 8:-8455728 MsaT041554.1:CDS
AAAATGGTGGTTCAAATTGT+TGG 0.451811 8:-8455648 MsaT041554.1:CDS
TTTCTTGTTCCACCACCAAC+TGG 0.453104 8:+8456024 None:intergenic
CCGGTTGTGAATACGGATTT+GGG 0.453312 8:-8455721 MsaT041554.1:CDS
TGCAAAAGATATTGGACTAA+AGG 0.473852 8:-8456066 MsaT041554.1:CDS
CCACCAAAATTCCTCTCACC+CGG 0.474564 8:+8455862 None:intergenic
TCTGGAAAATTTGGTTTCAT+GGG 0.485487 8:+8456189 None:intergenic
TACGGATTTGGGTCATAACC+CGG 0.489454 8:-8455710 MsaT041554.1:CDS
TTGAAGGTCTGAATTCAAAT+GGG 0.494568 8:-8455789 MsaT041554.1:CDS
GGTCTGAATTCAAATGGGTC+GGG 0.510843 8:-8455784 MsaT041554.1:CDS
TTTCTTGGTTTGCAAAATGG+TGG 0.514745 8:-8455661 MsaT041554.1:CDS
AATTGTTGGAATGATAATCA+TGG 0.521992 8:-8455634 MsaT041554.1:CDS
AGGTCTGAATTCAAATGGGT+CGG 0.529995 8:-8455785 MsaT041554.1:CDS
GACCCAGATTCATGTTGACC+CGG 0.541258 8:-8455881 MsaT041554.1:CDS
GGTCAACATGAATCTGGGTC+GGG 0.542667 8:+8455884 None:intergenic
GATTCCCCATTTCTACCACC+CGG 0.543789 8:+8455691 None:intergenic
ACCCGGGTCAACATGAATCT+GGG 0.549644 8:+8455879 None:intergenic
TTCATGTTGACCCGGGTGAG+AGG 0.558997 8:-8455873 MsaT041554.1:CDS
AGGAACATACCAGTTGGTGG+TGG 0.578156 8:-8456033 MsaT041554.1:CDS
TTCTACTGATAAGTTGAGCT+AGG 0.579652 8:+8455586 None:intergenic
GGGTCAACATGAATCTGGGT+CGG 0.590125 8:+8455883 None:intergenic
CCAAATCCGTATTCACAACC+GGG 0.603508 8:+8455722 None:intergenic
TTAAGGAACATACCAGTTGG+TGG 0.605626 8:-8456036 MsaT041554.1:CDS
TGAGAATTTGAACTGTGACC+CGG 0.605705 8:-8455740 MsaT041554.1:CDS
AAAAGATATTGGACTAAAGG+TGG 0.610002 8:-8456063 MsaT041554.1:CDS
GAACTGGTTGTTGATGCTGA+TGG 0.611011 8:+8455946 None:intergenic
GCTTTAAGGAACATACCAGT+TGG 0.613785 8:-8456039 MsaT041554.1:CDS
GGTTTGAAAATGGGTGAATG+TGG 0.621389 8:-8455763 MsaT041554.1:CDS
GTTGGAATGATAATCATGGT+TGG 0.627491 8:-8455630 MsaT041554.1:CDS
ACGGATTTGGGTCATAACCC+GGG 0.629532 8:-8455709 MsaT041554.1:CDS
CACCAAAATTCCTCTCACCC+GGG 0.635635 8:+8455863 None:intergenic
ACCCAGATTCATGTTGACCC+GGG 0.637927 8:-8455880 MsaT041554.1:CDS
ATTCCCCATTTCTACCACCC+GGG 0.643173 8:+8455692 None:intergenic
GCTGATGGAGAAGATGAAGA+TGG 0.658893 8:+8455961 None:intergenic
TAACCCGGGTGGTAGAAATG+GGG 0.710853 8:-8455695 MsaT041554.1:CDS
GTGTTGCTTGATTCACAACG+TGG 0.731087 8:+8456147 None:intergenic
GATTTGGGTCATAACCCGGG+TGG 0.768929 8:-8455706 MsaT041554.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 8455588 8456208 8455588 ID=MsaG041554
Chr8 mRNA 8455588 8456208 8455588 ID=MsaT041554.1;Parent=MsaG041554
Chr8 exon 8455588 8456208 8455588 ID=MsaT041554.1.exon1;Parent=MsaT041554.1
Chr8 CDS 8455588 8456208 8455588 ID=cds.MsaT041554.1;Parent=MsaT041554.1
Gene Sequence

>MsaT041554.1

ATGAAACCAAATTTTCCAGAACAAGAACAACTCAAATGTCCACGTTGTGAATCAAGCAACACAAAATTCTGCTACTACAACAACTACAATCTCTCACAACCACGCCATTTTTGCAAAAACTGCAAAAGATATTGGACTAAAGGTGGCGCTTTAAGGAACATACCAGTTGGTGGTGGAACAAGAAAAGTTACAAAACGTTCTTCAAATTCTAAACGTTCAACAACACCATCTTCATCTTCTCCATCAGCATCAACAACCAGTTCTTCAGCAGCAAAAGTTTCAGCTTCAGTTTCAGAAACTGACCCGACCCAGATTCATGTTGACCCGGGTGAGAGGAATTTTGGTGGAGGGAGTTTTAGTTCACTTCTTGCATCAACTGAGCGATTTGGGAACCTTTTTGAAGGTCTGAATTCAAATGGGTCGGGTTTGAAAATGGGTGAATGTGGTGAGAATTTGAACTGTGACCCGGTTGTGAATACGGATTTGGGTCATAACCCGGGTGGTAGAAATGGGGAATCAGAGATCTTTCTTGGTTTGCAAAATGGTGGTTCAAATTGTTGGAATGATAATCATGGTTGGTCTGATCTTGCTATATACACTCCTAGCTCAACTTATCAGTAG
Protein sequence

>MsaT041554.1

MKPNFPEQEQLKCPRCESSNTKFCYYNNYNLSQPRHFCKNCKRYWTKGGALRNIPVGGGTRKVTKRSSNSKRSTTPSSSSPSASTTSSSAAKVSASVSETDPTQIHVDPGERNFGGGSFSSLLASTERFGNLFEGLNSNGSGLKMGECGENLNCDPVVNTDLGHNPGGRNGESEIFLGLQNGGSNCWNDNHGWSDLAIYTPSSTYQ*