AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041629

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041629 MtrunA17_Chr8g0342871 82.895 304 34 4 1 304 1 286 8.63e-174 482
MsaG041629 MtrunA17_Chr2g0329921 46.667 315 143 8 1 303 1 302 6.51e-80 244
MsaG041629 MtrunA17_Chr4g0054131 37.624 202 116 3 1 196 1 198 1.17e-33 125
MsaG041629 MtrunA17_Chr8g0384671 36.574 216 118 5 1 215 1 198 1.51e-33 124
MsaG041629 MtrunA17_Chr2g0293381 36.986 219 121 5 1 219 1 202 2.95e-33 124
MsaG041629 MtrunA17_Chr5g0401061 37.374 198 107 3 1 196 1 183 4.98e-33 123
MsaG041629 MtrunA17_Chr1g0166681 55.556 63 28 0 1 63 1 63 1.32e-18 82.0
MsaG041629 MtrunA17_Chr8g0383821 60.345 58 23 0 1 58 1 58 6.27e-18 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041629 AT1G17520.4 43.791 306 148 6 1 301 1 287 4.84e-66 209
MsaG041629 AT1G72740.2 41.613 310 140 7 1 301 1 278 1.89e-65 207
MsaG041629 AT1G17520.3 43.974 307 147 7 1 301 1 288 1.22e-64 205
MsaG041629 AT1G17520.1 42.949 312 148 7 1 301 1 293 1.54e-63 202
MsaG041629 AT1G72740.1 40.823 316 140 8 1 301 1 284 4.70e-63 201
MsaG041629 AT1G17520.2 43.131 313 147 8 1 301 1 294 4.84e-62 199
MsaG041629 AT1G49950.3 36.364 198 109 5 1 196 1 183 3.89e-30 115
MsaG041629 AT1G49950.1 36.364 198 109 5 1 196 1 183 3.89e-30 115
MsaG041629 AT1G49950.2 36.364 198 109 5 1 196 1 183 3.89e-30 115
MsaG041629 AT3G49850.1 34.328 201 115 3 1 198 1 187 6.76e-29 112
MsaG041629 AT3G49850.2 34.328 201 115 3 1 198 1 187 6.76e-29 112
MsaG041629 AT5G67580.2 34.694 196 119 3 1 196 1 187 4.59e-26 105
MsaG041629 AT5G67580.1 34.694 196 119 3 1 196 1 187 4.59e-26 105
MsaG041629 AT1G54260.1 33.161 193 109 3 129 301 2 194 3.04e-22 92.4
MsaG041629 AT1G54240.1 28.736 174 113 5 135 301 57 226 2.80e-11 62.8

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGCTCTGACTACATTAA+AGG 0.241020 8:-9443983 MsaT041629.1:CDS
AAAGACATGGCCCTGGTAAA+TGG 0.273347 8:-9445775 MsaT041629.1:CDS
GAGACTGATGATTGCCGTTT+CGG 0.334617 8:+9442539 None:intergenic
AACTGAAAACAAATCATATC+TGG 0.352692 8:-9442447 MsaT041629.1:CDS
CAAGATAAAAGAAGCCTTGT+TGG 0.360419 8:-9442633 MsaT041629.1:CDS
GCAGCAGGAGGTTTGCATTT+GGG 0.365666 8:+9445549 None:intergenic
TGGGAAATCAAAAGCTGAAA+TGG 0.385077 8:-9445835 MsaT041629.1:CDS
TACATTGTTCATAAAGTTGT+TGG 0.388733 8:+9442351 None:intergenic
AGCAGCAGGAGGTTTGCATT+TGG 0.389149 8:+9445548 None:intergenic
ACTACATTAAAGGATACTAA+TGG 0.429817 8:-9443973 MsaT041629.1:CDS
ACAAGAGCAGTAGCAGGAAC+AGG 0.439457 8:+9445516 None:intergenic
ACATTGTTCATAAAGTTGTT+GGG 0.441124 8:+9442352 None:intergenic
TGCGCGGTGAAACTATCAAA+TGG 0.448048 8:-9442232 MsaT041629.1:CDS
ATAATATTGCTCAAGTTCCT+AGG 0.450675 8:-9445575 MsaT041629.1:CDS
AACCTCAATGAAAATAGAAA+TGG 0.452194 8:+9443936 None:intergenic
ACAGGAGAAGCAGCAGCAGC+AGG 0.461966 8:+9445534 None:intergenic
ATTCTCCTTGACCCTCAGTT+CGG 0.477510 8:-9445746 MsaT041629.1:CDS
AGAATGGCTTTCCATTTACC+AGG 0.481104 8:+9445764 None:intergenic
GAAGAAACAAGAGCAGTAGC+AGG 0.483938 8:+9445510 None:intergenic
CATCAAAACAAAAGGTGGTC+AGG 0.488212 8:-9442562 MsaT041629.1:CDS
ATCACCTTCACCAAAACAAA+AGG 0.490999 8:-9442603 MsaT041629.1:CDS
AAAAGGTGGTCAGGCCGAAA+CGG 0.494784 8:-9442553 MsaT041629.1:CDS
GAACTGAGGGTCAAGGAGAA+TGG 0.501381 8:+9445748 None:intergenic
ATCACCTTCATCAAAACAAA+AGG 0.502694 8:-9442570 MsaT041629.1:CDS
GGAGGAACAATTTGAAGAGG+AGG 0.508616 8:+9445420 None:intergenic
GAAGAAGACGCTCTTATCGC+TGG 0.509747 8:-9445803 MsaT041629.1:CDS
GCGCGGTGAAACTATCAAAT+GGG 0.518902 8:-9442231 MsaT041629.1:CDS
AGTCACTGACTGCAAGGTTG+AGG 0.520186 8:-9443592 MsaT041629.1:CDS
ATTGCTGCAGCAAAAGCATC+AGG 0.521800 8:-9443633 MsaT041629.1:intron
TGGTCAATTTGAAGAGGAGG+AGG 0.538373 8:+9445402 None:intergenic
TTGAGGATGCTTGTGAGTCA+AGG 0.546963 8:-9443575 MsaT041629.1:CDS
GGTAACCTTGAGGTCGATGT+TGG 0.557186 8:+9445703 None:intergenic
TCAGAAAGTCACTGACTGCA+AGG 0.561065 8:-9443598 MsaT041629.1:CDS
TTAACAACAACATCAGAAGA+AGG 0.563592 8:+9445453 None:intergenic
GAGTCAAGGGAAGCTTGAAA+AGG 0.565631 8:-9443561 MsaT041629.1:intron
GGTTTGCATTTGGGAAACCT+AGG 0.568248 8:+9445558 None:intergenic
TCGTTCCAACATCGACCTCA+AGG 0.571236 8:-9445708 MsaT041629.1:intron
GGAGGAGGAACAATTTGAAG+AGG 0.571972 8:+9445417 None:intergenic
TGAGGATGCTTGTGAGTCAA+GGG 0.572315 8:-9443574 MsaT041629.1:CDS
CAAATCATATCTGGCAACTG+AGG 0.579566 8:-9442438 MsaT041629.1:CDS
TCAATTTATGCAGGTTTGCG+CGG 0.581480 8:-9442248 MsaT041629.1:intron
TCTTGGTCAATTTGAAGAGG+AGG 0.585658 8:+9445399 None:intergenic
ACATCTTGGTCAATTTGAAG+AGG 0.588942 8:+9445396 None:intergenic
AACTGAGGCAGTCAAGGCGA+CGG 0.589786 8:-9442423 MsaT041629.1:CDS
TCTGGCAACTGAGGCAGTCA+AGG 0.592576 8:-9442429 MsaT041629.1:CDS
TTGGAACGAGAAGTGAGTAA+AGG 0.596141 8:+9445722 None:intergenic
GGAATCGAAAGACATGGCCC+TGG 0.601309 8:-9445782 MsaT041629.1:CDS
AGTCTCTGATGATATGCTGA+AGG 0.602271 8:-9442504 MsaT041629.1:CDS
GGAGAAGCAGCAGCAGCAGG+AGG 0.608517 8:+9445537 None:intergenic
ACCTTCATCAAAACAAAAGG+TGG 0.621310 8:-9442567 MsaT041629.1:CDS
AAGGACCGAACTGAGGGTCA+AGG 0.625130 8:+9445741 None:intergenic
ACCTTCACCAAAACAAAAGG+AGG 0.631938 8:-9442600 MsaT041629.1:CDS
TGAGTAAAGGACCGAACTGA+GGG 0.636132 8:+9445735 None:intergenic
GAATGGCTTTCCATTTACCA+GGG 0.646220 8:+9445765 None:intergenic
GTGAGTAAAGGACCGAACTG+AGG 0.646832 8:+9445734 None:intergenic
ATCGCTGGAATCGAAAGACA+TGG 0.677427 8:-9445788 MsaT041629.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 9442226 9445858 9442226 ID=MsaG041629
Chr8 mRNA 9442226 9445858 9442226 ID=MsaT041629.1;Parent=MsaG041629
Chr8 exon 9442226 9442257 9442226 ID=MsaT041629.1.exon6;Parent=MsaT041629.1
Chr8 CDS 9442226 9442257 9442226 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Chr8 exon 9442354 9442669 9442354 ID=MsaT041629.1.exon5;Parent=MsaT041629.1
Chr8 CDS 9442354 9442669 9442354 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Chr8 exon 9443562 9443645 9443562 ID=MsaT041629.1.exon4;Parent=MsaT041629.1
Chr8 CDS 9443562 9443645 9443562 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Chr8 exon 9443939 9444023 9443939 ID=MsaT041629.1.exon3;Parent=MsaT041629.1
Chr8 CDS 9443939 9444023 9443939 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Chr8 exon 9445375 9445622 9445375 ID=MsaT041629.1.exon2;Parent=MsaT041629.1
Chr8 CDS 9445375 9445622 9445375 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Chr8 exon 9445709 9445858 9445709 ID=MsaT041629.1.exon1;Parent=MsaT041629.1
Chr8 CDS 9445709 9445858 9445709 ID=cds.MsaT041629.1;Parent=MsaT041629.1
Gene Sequence

>MsaT041629.1

ATGGGAAATCAAAAGCTGAAATGGACGAATGAAGAAGAAGACGCTCTTATCGCTGGAATCGAAAGACATGGCCCTGGTAAATGGAAAGCCATTCTCCTTGACCCTCAGTTCGGTCCTTTACTCACTTCTCGTTCCAACATCGACCTCAAGGATAAATGGAGGAATATGAATGTGAATAATATTGCTCAAGTTCCTAGGTTTCCCAAATGCAAACCTCCTGCTGCTGCTGCTTCTCCTGTTCCTGCTACTGCTCTTGTTTCTTCTTCCAGTGCTGCTGTTAATCAAAATGTTGCTCTTCCTTCTTCTGATGTTGTTGTTAACAATGTTACTCCTCCTCTTCAAATTGTTCCTCCTCCTCTTCAAATTGACCAAGATGTTGTTAAAAACAACACACCTAGGTATGATGTCATGATTTATGAAGCTCTGACTACATTAAAGGATACTAATGGATCTGACGTAAATGCCATTTCTATTTTCATTGAGCAAAAGCATCAGGTTCCTCAAAATTTCAGAAAGTCACTGACTGCAAGGTTGAGGATGCTTGTGAGTCAAGGGAAGCTTGAAAAGGAACAAAATTGTTTCAAGATAAAAGAAGCCTTGTTGGTGAAAAAATCACCTTCACCAAAACAAAAGGAGGCCAAGCCATCACCTTCATCAAAACAAAAGGTGGTCAGGCCGAAACGGCAATCATCAGTCTCTGATGATTCATCAGTCTCTGATGATATGCTGAAGGAAGCAGCTGAAACTGCAGCATACATAATTGCTGAAACTGAAAACAAATCATATCTGGCAACTGAGGCAGTCAAGGCGACGGAAAAATACTCACGCATGGCTGAAGAAAATGATGCGATGTTGTTGCTAGCCCAACAACTTTATGAACAATGTTTGCGCGGTGAAACTATCAAATGGGCTTAA
Protein sequence

>MsaT041629.1

MGNQKLKWTNEEEDALIAGIERHGPGKWKAILLDPQFGPLLTSRSNIDLKDKWRNMNVNNIAQVPRFPKCKPPAAAASPVPATALVSSSSAAVNQNVALPSSDVVVNNVTPPLQIVPPPLQIDQDVVKNNTPRYDVMIYEALTTLKDTNGSDVNAISIFIEQKHQVPQNFRKSLTARLRMLVSQGKLEKEQNCFKIKEALLVKKSPSPKQKEAKPSPSSKQKVVRPKRQSSVSDDSSVSDDMLKEAAETAAYIIAETENKSYLATEAVKATEKYSRMAEENDAMLLLAQQLYEQCLRGETIKWA*