AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041662

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041662 MtrunA17_Chr8g0343441 99.219 256 1 1 1 255 1 256 0.0 526
MsaG041662 MtrunA17_Chr1g0150821 78.682 258 44 3 7 255 8 263 4.49e-122 348
MsaG041662 MtrunA17_Chr2g0329821 79.675 246 48 2 10 254 6 250 2.46e-120 343
MsaG041662 MtrunA17_Chr2g0300241 73.518 253 58 4 5 255 8 253 6.77e-120 342
MsaG041662 MtrunA17_Chr4g0076771 73.361 244 63 2 9 251 10 252 1.00e-115 332
MsaG041662 MtrunA17_Chr4g0006381 62.903 248 81 5 8 255 6 242 1.19e-94 278
MsaG041662 MtrunA17_Chr7g0242511 60.887 248 86 6 8 255 6 242 3.73e-92 271
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041662 AT5G20510.1 71.094 256 68 3 5 255 6 260 1.38e-120 345
MsaG041662 AT3G42790.1 73.896 249 57 5 7 254 8 249 1.77e-119 341
MsaG041662 AT1G14510.1 69.106 246 73 2 9 254 9 251 2.25e-109 316
MsaG041662 AT2G02470.1 67.829 258 78 3 1 255 1 256 7.15e-109 315
MsaG041662 AT2G02470.2 67.451 255 75 3 1 255 1 247 6.29e-108 312
MsaG041662 AT5G26210.1 78.039 255 54 2 1 254 1 254 7.93e-105 304
MsaG041662 AT5G05610.2 63.934 244 76 4 8 251 7 238 4.94e-99 289
MsaG041662 AT5G05610.1 63.934 244 76 4 8 251 7 238 4.94e-99 289
MsaG041662 AT3G11200.1 63.454 249 82 4 3 251 4 243 3.01e-95 280
MsaG041662 AT5G20510.2 69.585 217 60 3 44 255 4 219 4.26e-94 276
MsaG041662 AT5G20510.3 74.843 159 40 0 6 164 7 165 1.16e-81 243
MsaG041662 AT3G11200.2 62.500 216 72 4 36 251 24 230 2.12e-79 239
MsaG041662 AT2G02470.3 74.051 158 40 1 1 158 1 157 5.49e-71 218

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTGCTTAACCTGAGGTTC+TGG 0.298478 8:+10212969 None:intergenic
TCAATATTTGAAGTTGTTAC+TGG 0.306554 8:-10213883 MsaT041662.1:CDS
TACTACTTTGGTGCCAGATT+TGG 0.319173 8:-10214073 MsaT041662.1:CDS
TGCTTGGCATTAACTTTGCC+AGG 0.326435 8:-10214168 MsaT041662.1:CDS
AGAGAAAGACTGGCTATCTT+TGG 0.329748 8:-10214134 MsaT041662.1:CDS
TCCTCCACCTCATCATCTTT+AGG 0.341682 8:+10212930 None:intergenic
AGATGATGAGGTGGAGGAAC+TGG 0.383921 8:-10212925 MsaT041662.1:CDS
AGCACAGGCTCGGGAAGCTC+TGG 0.385581 8:+10214187 None:intergenic
TACCGCTTTCGAGCTGGACT+TGG 0.388039 8:+10213801 None:intergenic
ATTATGGCACTGATGAATTC+TGG 0.392260 8:-10212848 MsaT041662.1:CDS
AGATTTGTCGAAGCCAAATC+TGG 0.397894 8:+10214060 None:intergenic
GGCATTAACTTTGCCAGGGA+TGG 0.411593 8:-10214163 MsaT041662.1:CDS
GAGCTTCCCGAGCCTGTGCT+TGG 0.413554 8:-10214184 MsaT041662.1:CDS
TTGATATGCTCAGCCCTAGC+AGG 0.419284 8:+10212771 None:intergenic
TTTCTCTTGCATGCCATCCC+TGG 0.429424 8:+10214150 None:intergenic
AAGGAGAACCTGTGCTTGTA+TGG 0.431624 8:-10214259 MsaT041662.1:CDS
TTGCTGTTCACAGTGATTCA+TGG 0.433165 8:-10214111 MsaT041662.1:CDS
GGTGCATGCGGTGAGCATTA+TGG 0.433315 8:-10212864 MsaT041662.1:CDS
GATCATTGATCATGTTGAAC+AGG 0.434639 8:+10213911 None:intergenic
GTTCTATACCCAATGTGATC+CGG 0.448947 8:-10214824 MsaT041662.1:CDS
ATCGCCATTGCGTACTACTT+TGG 0.461307 8:-10214085 MsaT041662.1:CDS
TTGACTTCCCACTGCTCACT+AGG 0.465024 8:+10214232 None:intergenic
ATTATTTACCGCTTTCGAGC+TGG 0.469549 8:+10213795 None:intergenic
ATCTGCGAGAAGTGGTTCCA+TGG 0.475159 8:-10212813 MsaT041662.1:CDS
CTCTTGTTGTTGCTGCATGA+TGG 0.477879 8:+10212735 None:intergenic
TAACTTACCCGGATCACATT+GGG 0.487332 8:+10214816 None:intergenic
CTCACCGCATGCACCACATA+AGG 0.495356 8:+10212872 None:intergenic
GTAACAACTTCAAATATTGA+TGG 0.502359 8:+10213886 None:intergenic
TTCTATACCCAATGTGATCC+GGG 0.502678 8:-10214823 MsaT041662.1:intron
GTTAATGCCAAGCACAGGCT+CGG 0.508894 8:+10214177 None:intergenic
GGCTTTGTCTGCTTAACCTG+AGG 0.513964 8:+10212963 None:intergenic
TGATATGCTCAGCCCTAGCA+GGG 0.516021 8:+10212772 None:intergenic
GTGTGAAGATCACCCCTGCT+AGG 0.516439 8:-10212785 MsaT041662.1:CDS
GCAGCGGGCTCCAGAACCTC+AGG 0.519888 8:-10212979 MsaT041662.1:intron
CTCATCAAAGCTCTCACCAC+TGG 0.525319 8:-10216343 MsaT041662.1:intron
ATGGCATGCAAGAGAAAGAC+TGG 0.534454 8:-10214144 MsaT041662.1:CDS
TCTGGCGGAACTTCCTCAGC+AGG 0.540129 8:+10214205 None:intergenic
ACAGGCTCGGGAAGCTCTGG+CGG 0.540889 8:+10214190 None:intergenic
ACCTAAAGATGATGAGGTGG+AGG 0.542306 8:-10212931 MsaT041662.1:CDS
TTAATGCCAAGCACAGGCTC+GGG 0.544159 8:+10214178 None:intergenic
GGTTAAGCAGACAAAGCCAT+TGG 0.555690 8:-10212958 MsaT041662.1:CDS
AAAACTTCCTCAACGGTGCG+TGG 0.559807 8:+10216394 None:intergenic
TGACTTCCCACTGCTCACTA+GGG 0.569521 8:+10214233 None:intergenic
TGATCTTCACACATTTCCCA+TGG 0.574011 8:+10212796 None:intergenic
ATCTCTGAAAACTTCCTCAA+CGG 0.574665 8:+10216387 None:intergenic
TGGCTTCCCTAGTGAGCAGT+GGG 0.576162 8:-10214239 MsaT041662.1:CDS
GGAAGTCAATTTACCTGCTG+AGG 0.576632 8:-10214218 MsaT041662.1:CDS
TGAGGTGGAGGAACTGGACG+AGG 0.577577 8:-10212919 MsaT041662.1:CDS
GCAAAGTTAATGCCAAGCAC+AGG 0.578595 8:+10214172 None:intergenic
TAGGGAAGCCATACAAGCAC+AGG 0.583243 8:+10214251 None:intergenic
TTGGCTTCGACAAATCTGAC+AGG 0.592431 8:-10214054 MsaT041662.1:intron
TCATCTTTAGGTAACTCCAA+TGG 0.594683 8:+10212942 None:intergenic
TGTGAAGATCACCCCTGCTA+GGG 0.600442 8:-10212784 MsaT041662.1:CDS
GCTTGGCATTAACTTTGCCA+GGG 0.610233 8:-10214167 MsaT041662.1:CDS
GCTGTGACATCTGCGAGAAG+TGG 0.611408 8:-10212821 MsaT041662.1:CDS
TCTGCGAGAAGTGGTTCCAT+GGG 0.616851 8:-10212812 MsaT041662.1:CDS
GGAGTTACCTAAAGATGATG+AGG 0.622708 8:-10212937 MsaT041662.1:CDS
GTAACTTACCCGGATCACAT+TGG 0.626802 8:+10214815 None:intergenic
GTATAATCCACGCACCGTTG+AGG 0.643429 8:-10216401 MsaT041662.1:CDS
GGCACCAAAGTAGTACGCAA+TGG 0.648228 8:+10214081 None:intergenic
ATGGCTTCCCTAGTGAGCAG+TGG 0.650269 8:-10214240 MsaT041662.1:CDS
GAACATGGAGAGACCTTATG+TGG 0.650525 8:-10212885 MsaT041662.1:CDS
ACTTGGATTTGCTGCCACTG+TGG 0.654768 8:+10213818 None:intergenic
TGCAGCAAAGAAACAAGTCA+AGG 0.656590 8:-10213860 MsaT041662.1:CDS
GTTACCTAAAGATGATGAGG+TGG 0.657704 8:-10212934 MsaT041662.1:CDS
GAGGAAGATGAAGATGAACA+TGG 0.668055 8:-10212900 MsaT041662.1:CDS
GATATGCTCAGCCCTAGCAG+GGG 0.690376 8:+10212773 None:intergenic
GAGACCTTATGTGGTGCATG+CGG 0.712511 8:-10212876 MsaT041662.1:CDS
TCTTCAGTTTCAAACCACAG+TGG 0.768394 8:-10213832 MsaT041662.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 10212722 10216440 10212722 ID=MsaG041662
Chr8 mRNA 10212722 10216440 10212722 ID=MsaT041662.1;Parent=MsaG041662
Chr8 exon 10212722 10212997 10212722 ID=MsaT041662.1.exon5;Parent=MsaT041662.1
Chr8 CDS 10212722 10212997 10212722 ID=cds.MsaT041662.1;Parent=MsaT041662.1
Chr8 exon 10213807 10213939 10213807 ID=MsaT041662.1.exon4;Parent=MsaT041662.1
Chr8 CDS 10213807 10213939 10213807 ID=cds.MsaT041662.1;Parent=MsaT041662.1
Chr8 exon 10214055 10214283 10214055 ID=MsaT041662.1.exon3;Parent=MsaT041662.1
Chr8 CDS 10214055 10214283 10214055 ID=cds.MsaT041662.1;Parent=MsaT041662.1
Chr8 exon 10214824 10214856 10214824 ID=MsaT041662.1.exon2;Parent=MsaT041662.1
Chr8 CDS 10214824 10214856 10214824 ID=cds.MsaT041662.1;Parent=MsaT041662.1
Chr8 exon 10216344 10216440 10216344 ID=MsaT041662.1.exon1;Parent=MsaT041662.1
Chr8 CDS 10216344 10216440 10216344 ID=cds.MsaT041662.1;Parent=MsaT041662.1
Gene Sequence

>MsaT041662.1

ATGGACGCGCGCACACAGTATAATCCACGCACCGTTGAGGAAGTTTTCAGAGATTTTAAGGGTCGTCGTGCTGGTCTCATCAAAGCTCTCACCACTGATGTTGAAGAGTTCTATACCCAATGTGATCCGGAGAAGGAGAACCTGTGCTTGTATGGCTTCCCTAGTGAGCAGTGGGAAGTCAATTTACCTGCTGAGGAAGTTCCGCCAGAGCTTCCCGAGCCTGTGCTTGGCATTAACTTTGCCAGGGATGGCATGCAAGAGAAAGACTGGCTATCTTTGGTTGCTGTTCACAGTGATTCATGGTTAATCGCCATTGCGTACTACTTTGGTGCCAGATTTGGCTTCGACAAATCTGACAGGAAGCGCCTGTTCAACATGATCAATGATCTGCCATCAATATTTGAAGTTGTTACTGGTGCAGCAAAGAAACAAGTCAAGGAGAAGTCTTCAGTTTCAAACCACAGTGGCAGCAAATCCAAGTCCAGCTCGAAACGGGCTCCAGAACCTCAGGTTAAGCAGACAAAGCCATTGGAGTTACCTAAAGATGATGAGGTGGAGGAACTGGACGAGGAAGATGAAGATGAACATGGAGAGACCTTATGTGGTGCATGCGGTGAGCATTATGGCACTGATGAATTCTGGATTTGCTGTGACATCTGCGAGAAGTGGTTCCATGGGAAATGTGTGAAGATCACCCCTGCTAGGGCTGAGCATATCAAGCAATACAAGTGTCCATCATGCAGCAACAACAAGAGAGCTCGCCCTTGA
Protein sequence

>MsaT041662.1

MDARTQYNPRTVEEVFRDFKGRRAGLIKALTTDVEEFYTQCDPEKENLCLYGFPSEQWEVNLPAEEVPPELPEPVLGINFARDGMQEKDWLSLVAVHSDSWLIAIAYYFGARFGFDKSDRKRLFNMINDLPSIFEVVTGAAKKQVKEKSSVSNHSGSKSKSSSKRAPEPQVKQTKPLELPKDDEVEELDEEDEDEHGETLCGACGEHYGTDEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPSCSNNKRARP*