AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041664

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041664 MtrunA17_Chr8g0343451 90.312 320 22 4 1 313 1 318 0.0 547
MsaG041664 MtrunA17_Chr2g0329811 53.293 334 118 11 7 313 23 345 1.66e-92 278
MsaG041664 MtrunA17_Chr2g0300261 48.404 188 86 6 106 282 17 204 4.41e-45 153
MsaG041664 MtrunA17_Chr2g0292411 63.333 90 32 1 133 222 85 173 5.27e-34 123
MsaG041664 MtrunA17_Chr8g0352251 47.651 149 60 5 144 275 136 283 7.91e-32 120
MsaG041664 MtrunA17_Chr3g0103391 54.902 102 36 2 120 217 131 226 2.50e-31 119
MsaG041664 MtrunA17_Chr1g0177091 49.573 117 44 4 107 221 120 223 5.82e-27 108
MsaG041664 MtrunA17_Chr7g0267581 49.153 118 45 4 107 222 122 226 1.45e-26 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041664 AT3G14020.2 44.884 303 131 12 4 289 17 300 2.74e-59 192
MsaG041664 AT3G14020.1 44.884 303 131 12 4 289 17 300 2.74e-59 192
MsaG041664 AT1G72830.1 37.651 332 167 11 4 311 23 338 9.98e-52 174
MsaG041664 AT1G72830.2 37.538 333 167 12 4 311 23 339 5.87e-50 169
MsaG041664 AT1G17590.3 43.651 252 109 9 74 311 94 326 2.73e-48 164
MsaG041664 AT1G17590.2 43.651 252 109 9 74 311 94 326 2.73e-48 164
MsaG041664 AT1G17590.4 43.651 252 109 9 74 311 94 326 2.73e-48 164
MsaG041664 AT1G17590.1 43.651 252 109 9 74 311 94 326 2.73e-48 164
MsaG041664 AT1G54160.1 45.957 235 106 7 7 225 12 241 4.32e-47 161
MsaG041664 AT1G30500.2 52.459 122 57 1 119 240 58 178 6.55e-38 133
MsaG041664 AT1G72830.3 33.435 329 160 9 4 311 23 313 1.52e-37 136
MsaG041664 AT1G30500.1 50.820 122 55 1 119 240 58 174 4.14e-36 129
MsaG041664 AT2G34720.1 45.255 137 64 3 110 239 44 176 1.10e-31 117
MsaG041664 AT2G34720.2 47.328 131 58 3 116 239 15 141 1.87e-30 113
MsaG041664 AT2G34720.3 47.328 131 58 3 116 239 15 141 1.87e-30 113
MsaG041664 AT5G12840.4 45.802 131 57 4 99 224 112 233 3.75e-30 115
MsaG041664 AT5G12840.2 45.802 131 57 4 99 224 112 233 3.75e-30 115
MsaG041664 AT5G12840.5 45.802 131 57 4 99 224 113 234 3.75e-30 115
MsaG041664 AT5G12840.3 45.802 131 57 4 99 224 113 234 3.75e-30 115
MsaG041664 AT5G12840.1 45.802 131 57 4 99 224 113 234 3.75e-30 115
MsaG041664 AT3G20910.1 48.413 126 47 5 101 217 105 221 8.85e-30 115
MsaG041664 AT3G05690.1 46.667 135 50 6 104 230 90 210 4.20e-24 99.8
MsaG041664 AT5G06510.1 49.138 116 45 5 107 221 88 190 6.80e-24 99.0
MsaG041664 AT5G06510.5 49.138 116 45 5 107 221 88 190 6.80e-24 99.0
MsaG041664 AT5G06510.2 49.138 116 45 5 107 221 88 190 6.80e-24 99.0
MsaG041664 AT5G06510.6 49.138 116 45 5 107 221 88 190 6.80e-24 99.0
MsaG041664 AT3G05690.2 46.667 135 50 6 104 230 127 247 8.99e-24 99.8
MsaG041664 AT5G06510.3 51.351 111 40 5 112 221 44 141 1.39e-23 96.7
MsaG041664 AT5G06510.4 54.369 103 33 5 120 221 6 95 1.48e-23 95.5
MsaG041664 AT5G06510.7 54.369 103 33 5 120 221 6 95 1.48e-23 95.5

Find 78 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTATACTGGACAGAAAT+TGG 0.227693 8:+10231392 None:intergenic
TGCTTCGCATTCACATAAAT+TGG 0.276367 8:+10230429 None:intergenic
AACTGGTCGCCAATCTTATC+TGG 0.308062 8:-10232196 MsaT041664.1:CDS
TACCTTGCCTCCTTCTCAAC+TGG 0.319759 8:-10231285 MsaT041664.1:CDS
CGGATCATCTGCAGGGAGTC+AGG 0.383305 8:-10231312 MsaT041664.1:CDS
ACATTATTCCGTGATCCATA+TGG 0.396988 8:+10230946 None:intergenic
GGTTCCTCACAAAGATTAAG+AGG 0.418602 8:+10230450 None:intergenic
TGTTCAACACTTAGCGAAAC+CGG 0.430982 8:-10231359 MsaT041664.1:CDS
TGAAGTCGGATTCATGTTGT+TGG 0.453276 8:+10230009 None:intergenic
ATTGAGTAGAAGAAGAATCT+TGG 0.456059 8:+10232220 None:intergenic
CATGCTGAGACATACTCTAA+TGG 0.462998 8:-10230982 MsaT041664.1:CDS
TGGCGCGACTCGTGTAGATA+TGG 0.466406 8:+10230248 None:intergenic
AAGCAGAGGAATTGTTATAC+TGG 0.467814 8:+10231381 None:intergenic
TGGTTACCCTTCTCATATGC+AGG 0.470689 8:-10229979 MsaT041664.1:CDS
GGCCTACTAGCAGCTCCATA+TGG 0.471924 8:-10230961 MsaT041664.1:CDS
TCTTCTTCTACTCAATCAAC+TGG 0.473460 8:-10232213 MsaT041664.1:CDS
CTAATCATGTCTCTCACATA+AGG 0.480287 8:+10229901 None:intergenic
GGTATCATCGGATCATCTGC+AGG 0.483140 8:-10231320 MsaT041664.1:CDS
GCCGATGCCGGTGGTGGTGG+TGG 0.483222 8:-10229948 MsaT041664.1:CDS
GTGGAAATGTGCAGGAATCA+AGG 0.488946 8:-10230118 MsaT041664.1:CDS
TCAGCCGATGCCGGTGGTGG+TGG 0.489227 8:-10229951 MsaT041664.1:CDS
TTGAAGAGAGCTAGAGGCTC+TGG 0.491508 8:-10230215 MsaT041664.1:CDS
CGCCAATCTTATCTGGAAGT+TGG 0.493422 8:-10232189 MsaT041664.1:intron
CAGATTCAACTGAGTGGTGG+TGG 0.501293 8:+10230142 None:intergenic
AACCTAGTGGTTTAGACTTG+TGG 0.508102 8:+10232256 None:intergenic
TTGTCAGCCGATGCCGGTGG+TGG 0.509405 8:-10229954 MsaT041664.1:CDS
TAGCAGGAATGCCTCCCGTT+CGG 0.509646 8:-10230482 MsaT041664.1:CDS
ATTCCGACCACCACCACCAC+CGG 0.525135 8:+10229941 None:intergenic
CAATGAAACACTCATGGCCA+TGG 0.528292 8:-10232362 MsaT041664.1:CDS
CAGGATTTGTCAGCCGATGC+CGG 0.529122 8:-10229960 MsaT041664.1:CDS
TCTCTCACATAAGGACAGAT+AGG 0.538450 8:+10229910 None:intergenic
ACCTTCCACACTCTTTCCGC+CGG 0.539251 8:+10231340 None:intergenic
AACCAACTTCCAGATAAGAT+TGG 0.540368 8:+10232187 None:intergenic
GTTCATCATACTCAGCTAGC+AGG 0.544741 8:-10230498 MsaT041664.1:CDS
AGAGGCAAAGGAATCCGAAC+GGG 0.545487 8:+10230468 None:intergenic
TTTGCCTCTTAATCTTTGTG+AGG 0.548183 8:-10230454 MsaT041664.1:CDS
GACAAATCCTGCATATGAGA+AGG 0.557039 8:+10229972 None:intergenic
TCACAAAGATTAAGAGGCAA+AGG 0.559654 8:+10230456 None:intergenic
TGGAAATGTGCAGGAATCAA+GGG 0.560891 8:-10230117 MsaT041664.1:CDS
ATCCATATGGAGCTGCTAGT+AGG 0.561398 8:+10230959 None:intergenic
CTCTCTTCAATGCATGCAGA+TGG 0.566697 8:+10230228 None:intergenic
AATGAAACACTCATGGCCAT+GGG 0.573475 8:-10232361 MsaT041664.1:CDS
ACTTGTGACGTCGGAACAAG+TGG 0.585137 8:+10230055 None:intergenic
GAATCTGAGTGGAAATGTGC+AGG 0.587768 8:-10230126 MsaT041664.1:CDS
TTAGAGTATGTCTCAGCATG+AGG 0.588380 8:+10230985 None:intergenic
CAAGTAGAAAAGTATAGAGA+TGG 0.594749 8:-10230089 MsaT041664.1:CDS
CGAAACCGGCGGAAAGAGTG+TGG 0.602019 8:-10231345 MsaT041664.1:CDS
ACCGGCGGAAAGAGTGTGGA+AGG 0.603635 8:-10231341 MsaT041664.1:CDS
TTGTGATCCAGTTGAGAAGG+AGG 0.605135 8:+10231278 None:intergenic
AAGAGTGTGGAAGGTATCAT+CGG 0.608613 8:-10231332 MsaT041664.1:CDS
CATGCATTGAAGAGAGCTAG+AGG 0.616928 8:-10230221 MsaT041664.1:CDS
TGGTGGAAACATTAAAGCCA+TGG 0.621019 8:+10230159 None:intergenic
TGATTGTGATCCAGTTGAGA+AGG 0.622776 8:+10231275 None:intergenic
GCTAAGTGTTGAACAAGCAG+AGG 0.624669 8:+10231367 None:intergenic
TCTTGAAATTGAAAACCTAG+TGG 0.627398 8:+10232243 None:intergenic
GGAAGACAATGAAACACTCA+TGG 0.629376 8:-10232368 None:intergenic
ACAAATCCTGCATATGAGAA+GGG 0.629662 8:+10229973 None:intergenic
GATTTGTCAGCCGATGCCGG+TGG 0.632484 8:-10229957 MsaT041664.1:CDS
TAGCAGCTCCATATGGATCA+CGG 0.633412 8:-10230954 MsaT041664.1:CDS
GGCAAAGGAATCCGAACGGG+AGG 0.635841 8:+10230471 None:intergenic
ATCCAGTTGAGAAGGAGGCA+AGG 0.636669 8:+10231283 None:intergenic
TAGAGTATGTCTCAGCATGA+GGG 0.637829 8:+10230986 None:intergenic
ATTAGAAGCACTTGTGACGT+CGG 0.640775 8:+10230046 None:intergenic
GTCTACGTCTCATAATAGCA+TGG 0.645173 8:+10230403 None:intergenic
GAATCCGACTTCAGATCATG+TGG 0.653505 8:-10229999 MsaT041664.1:CDS
ATGAAACACTCATGGCCATG+GGG 0.654173 8:-10232360 MsaT041664.1:CDS
ACCACTCAGTTGAATCTGAG+TGG 0.655182 8:-10230137 MsaT041664.1:CDS
AAGAGAGCTAGAGGCTCTGG+CGG 0.658706 8:-10230212 MsaT041664.1:CDS
AAGAGGCAAAGGAATCCGAA+CGG 0.660223 8:+10230467 None:intergenic
GTAACCACATGATCTGAAGT+CGG 0.661286 8:+10229995 None:intergenic
TCCACTCAGATTCAACTGAG+TGG 0.667577 8:+10230136 None:intergenic
GACGTCGGAACAAGTGGTTG+TGG 0.668249 8:+10230061 None:intergenic
TGCCACAAGTCTAAACCACT+AGG 0.682061 8:-10232258 MsaT041664.1:CDS
ACTCAGATTCAACTGAGTGG+TGG 0.684029 8:+10230139 None:intergenic
GTATCATCGGATCATCTGCA+GGG 0.684338 8:-10231319 MsaT041664.1:CDS
TGAAACACTCATGGCCATGG+GGG 0.692226 8:-10232359 MsaT041664.1:CDS
CTCACATAAGGACAGATAGG+CGG 0.718305 8:+10229913 None:intergenic
TCAACACTTAGCGAAACCGG+CGG 0.741425 8:-10231356 MsaT041664.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 10229914 10232382 10229914 ID=MsaG041664
Chr8 mRNA 10229914 10232382 10229914 ID=MsaT041664.1;Parent=MsaG041664
Chr8 exon 10229914 10230270 10229914 ID=MsaT041664.1.exon5;Parent=MsaT041664.1
Chr8 CDS 10229914 10230270 10229914 ID=cds.MsaT041664.1;Parent=MsaT041664.1
Chr8 exon 10230353 10230520 10230353 ID=MsaT041664.1.exon4;Parent=MsaT041664.1
Chr8 CDS 10230353 10230520 10230353 ID=cds.MsaT041664.1;Parent=MsaT041664.1
Chr8 exon 10230948 10231022 10230948 ID=MsaT041664.1.exon3;Parent=MsaT041664.1
Chr8 CDS 10230948 10231022 10230948 ID=cds.MsaT041664.1;Parent=MsaT041664.1
Chr8 exon 10231268 10231416 10231268 ID=MsaT041664.1.exon2;Parent=MsaT041664.1
Chr8 CDS 10231268 10231416 10231268 ID=cds.MsaT041664.1;Parent=MsaT041664.1
Chr8 exon 10232190 10232382 10232190 ID=MsaT041664.1.exon1;Parent=MsaT041664.1
Chr8 CDS 10232190 10232382 10232190 ID=cds.MsaT041664.1;Parent=MsaT041664.1
Gene Sequence

>MsaT041664.1

ATGAAACACTCATGGCCATGGGGGACTTGTTCTGAATCTGATGTGCAACAATCCTCTATGTCAGAAAATCTAACTTTGAATATGAGTGTTTTGCAACAAGAATGCCACAAGTCTAAACCACTAGGTTTTCAATTTCAAGACCAAGATTCTTCTTCTACTCAATCAACTGGTCGCCAATCTTATCTGGAAGTTGGTCCAATTTCTGTCCAGTATAACAATTCCTCTGCTTGTTCAACACTTAGCGAAACCGGCGGAAAGAGTGTGGAAGGTATCATCGGATCATCTGCAGGGAGTCAGGATTTTACCTTGCCTCCTTCTCAACTGGATCACAATCAATCACTTGATCATGTTGCATTCCCTCATGCTGAGACATACTCTAATGGCCTACTAGCAGCTCCATATGGATCACGGAATAATGTTCATCATACTCAGCTAGCAGGAATGCCTCCCGTTCGGATTCCTTTGCCTCTTAATCTTTGTGAGGAACCAATTTATGTGAATGCGAAGCAGTACCATGCTATTATGAGACGTAGACAGTATCGTGCAAAGCTCGAAGCACAGAACAAACTCGTCAAAAATCGGAAACCATATCTACACGAGTCGCGCCATCTGCATGCATTGAAGAGAGCTAGAGGCTCTGGCGGACGTTTTCTAAATACCAAAAAGCTTCAAGACCATGGCTTTAATGTTTCCACCACCACTCAGTTGAATCTGAGTGGAAATGTGCAGGAATCAAGGGTACATCAAGTAGAAAAGTATAGAGATGGTGCTTCCACAACCACTTGTTCCGACGTCACAAGTGCTTCTAATAGTGACGACATGTTCCAACAACATGAATCCGACTTCAGATCATGTGGTTACCCTTCTCATATGCAGGATTTGTCAGCCGATGCCGGTGGTGGTGGTGGTCGGAATCAACACCGCCTATCTGTCCTTATGTGA
Protein sequence

>MsaT041664.1

MKHSWPWGTCSESDVQQSSMSENLTLNMSVLQQECHKSKPLGFQFQDQDSSSTQSTGRQSYLEVGPISVQYNNSSACSTLSETGGKSVEGIIGSSAGSQDFTLPPSQLDHNQSLDHVAFPHAETYSNGLLAAPYGSRNNVHHTQLAGMPPVRIPLPLNLCEEPIYVNAKQYHAIMRRRQYRAKLEAQNKLVKNRKPYLHESRHLHALKRARGSGGRFLNTKKLQDHGFNVSTTTQLNLSGNVQESRVHQVEKYRDGASTTTCSDVTSASNSDDMFQQHESDFRSCGYPSHMQDLSADAGGGGGRNQHRLSVLM*