AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041694


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041694 MtrunA17_Chr8g0343881 95.977 348 9 2 1 343 1 348 0.0 688
MsaG041694 MtrunA17_Chr4g0070591 44.134 358 154 16 1 333 1 337 2.11e-75 236
MsaG041694 MtrunA17_Chr2g0300511 42.075 347 157 12 7 333 3 325 1.44e-68 218
MsaG041694 MtrunA17_Chr4g0021261 38.415 328 147 12 10 333 24 300 4.60e-55 183
MsaG041694 MtrunA17_Chr1g0152191 52.846 123 54 1 1 119 134 256 1.09e-40 150
MsaG041694 MtrunA17_Chr5g0409471 58.333 108 33 2 12 119 17 112 2.14e-39 142
MsaG041694 MtrunA17_Chr7g0265931 54.472 123 46 4 12 132 79 193 8.53e-36 133
MsaG041694 MtrunA17_Chr3g0127641 47.706 109 39 2 12 120 113 203 2.42e-28 115
MsaG041694 MtrunA17_Chr8g0351521 36.424 151 79 2 8 158 51 184 5.86e-21 93.2
MsaG041694 MtrunA17_Chr8g0351521 52.727 55 26 0 75 129 302 356 1.00e-12 68.6
MsaG041694 MtrunA17_Chr8g0351291 64.103 39 14 0 75 113 51 89 1.23e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041694 AT3G13960.1 41.192 369 163 11 3 321 8 372 5.26e-71 226
MsaG041694 AT2G06200.2 58.915 129 38 3 2 117 36 162 3.38e-43 151
MsaG041694 AT2G06200.1 59.836 122 34 3 9 117 4 123 6.71e-43 149
MsaG041694 AT4G37740.1 50.000 152 55 4 12 145 165 313 2.90e-41 151
MsaG041694 AT2G22840.1 57.018 114 35 4 12 119 134 239 3.58e-35 134
MsaG041694 AT2G36400.1 51.181 127 47 4 10 127 75 195 8.52e-35 131
MsaG041694 AT3G52910.1 41.206 199 94 6 11 195 82 271 1.58e-32 125
MsaG041694 AT2G06200.3 88.235 51 4 1 69 117 1 51 2.84e-25 100
MsaG041694 AT5G53660.1 47.222 108 40 1 12 119 60 150 8.79e-25 103
MsaG041694 AT4G24150.3 41.739 115 61 2 10 119 110 223 2.21e-24 103
MsaG041694 AT4G24150.2 41.739 115 61 2 10 119 148 261 3.36e-24 103
MsaG041694 AT4G24150.1 37.143 140 57 3 10 119 148 286 4.95e-22 97.1
MsaG041694 AT2G45480.2 46.535 101 54 0 16 116 29 129 6.75e-16 77.8
MsaG041694 AT2G45480.3 46.535 101 54 0 16 116 29 129 1.99e-15 77.4
MsaG041694 AT2G45480.3 47.500 80 31 3 40 119 281 349 2.19e-14 74.3
MsaG041694 AT2G45480.1 46.535 101 54 0 16 116 27 127 2.03e-15 77.4
MsaG041694 AT2G45480.1 47.500 80 31 3 40 119 279 347 2.17e-14 74.3

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGCTTGAGTAAGACTTAA+AGG 0.217109 8:+10717660 None:intergenic
GGAGGCAAGTTCAAGGAATA+AGG 0.269692 8:-10717780 MsaT041694.1:CDS
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8:+10718451 None:intergenic
AAACCACTTCATCATTTCTT+TGG 0.279787 8:-10717514 MsaT041694.1:CDS
AGCCAGGAATCTGTGTTCTT+TGG 0.341985 8:+10717481 None:intergenic
CTTTCACAGTACTTTGAGTC+TGG 0.352106 8:+10718388 None:intergenic
CTAGATGAACCTGTAGCTTC+TGG 0.361439 8:+10717727 None:intergenic
CCCTTTCTCTACTTCTTCCA+TGG 0.361986 8:-10718206 MsaT041694.1:CDS
GAGTAGTAGCATCTTGAGTT+TGG 0.362099 8:+10718083 None:intergenic
GCTGTGTTTGTGTTATTTGT+TGG 0.366262 8:+10718295 None:intergenic
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8:+10718452 None:intergenic
GAGAGTAATAGTAAGGGTTT+TGG 0.368535 8:+10718146 None:intergenic
GCTGCTGTTGTTGTTGTTCA+AGG 0.381104 8:+10718249 None:intergenic
TATATGAGATCTGGTGGAAT+AGG 0.406485 8:+10718700 None:intergenic
AGAAAAGTAGACCCAGAGCC+AGG 0.407864 8:-10718463 MsaT041694.1:CDS
TCAAGTTCTTGCCATTGATT+TGG 0.410002 8:+10718763 None:intergenic
ATTGATTGAGAGTAATAGTA+AGG 0.410096 8:+10718139 None:intergenic
GAGCTTGAGTAAGACTTAAA+GGG 0.429522 8:+10717661 None:intergenic
GAAGAACAGATGGCAAGAAA+TGG 0.429768 8:-10718432 MsaT041694.1:CDS
CAGAAGCTACAGGTTCATCT+AGG 0.438969 8:-10717726 MsaT041694.1:CDS
TGAGTGCAAGTTCAAGAAAT+AGG 0.444135 8:-10718798 MsaT041694.1:CDS
ACTTTCTTTCCAGAAGCTAC+AGG 0.451806 8:-10717736 MsaT041694.1:CDS
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8:+10718445 None:intergenic
TTAATAGAGTATATGAGATC+TGG 0.465803 8:+10718691 None:intergenic
TAATCAACCTGTTGGAACTC+TGG 0.470845 8:+10717441 None:intergenic
CACCAAAGAACACAGATTCC+TGG 0.474579 8:-10717483 MsaT041694.1:CDS
CTCATGATGACAAGGACTAT+AGG 0.477649 8:-10718030 MsaT041694.1:intron
CAATACTTACTTGGTGGATG+AGG 0.492982 8:+10718631 None:intergenic
GCCATGGAAGAAGTAGAGAA+AGG 0.495080 8:+10718205 None:intergenic
ACCTGTTGGAACTCTGGAGT+TGG 0.501428 8:+10717447 None:intergenic
TCCAACTCCAGAGTTCCAAC+AGG 0.510080 8:-10717448 MsaT041694.1:intron
GTAGAGAAAGGGAAAGAAGA+AGG 0.511856 8:+10718217 None:intergenic
CCATGGAAGAAGTAGAGAAA+GGG 0.516371 8:+10718206 None:intergenic
TGATAAAGGAGGCAAGTTCA+AGG 0.519079 8:-10717787 MsaT041694.1:intron
GAGGCAAGTTCAAGGAATAA+GGG 0.523074 8:-10717779 MsaT041694.1:CDS
GTTCCACAGGCTTTCTTGAA+CGG 0.529120 8:+10718344 None:intergenic
TGTGAATGGTGCAGTTGGGT+GGG 0.535441 8:-10718509 MsaT041694.1:intron
TTCATCATTTCTTTGGTGAG+TGG 0.536164 8:-10717507 MsaT041694.1:CDS
GTTGGAAGCAAGATCTAGCC+AGG 0.536185 8:+10717465 None:intergenic
GTGAATGGTGCAGTTGGGTG+GGG 0.550789 8:-10718508 MsaT041694.1:intron
GAAAAGTAGACCCAGAGCCA+GGG 0.561252 8:-10718462 MsaT041694.1:CDS
TCACCAAAGAAATGATGAAG+TGG 0.571081 8:+10717511 None:intergenic
CTGTGAATGGTGCAGTTGGG+TGG 0.577092 8:-10718510 MsaT041694.1:intron
ATAGAGTATATGAGATCTGG+TGG 0.579210 8:+10718694 None:intergenic
GAAGAAACTACTAGTTCCAC+AGG 0.590035 8:+10718331 None:intergenic
CAAGAAATGGAGATGCTCAA+AGG 0.592887 8:-10718419 MsaT041694.1:CDS
TTGATTGAGAGTAATAGTAA+GGG 0.594306 8:+10718140 None:intergenic
GGGAGGTGCAGAAGAACAGA+TGG 0.600412 8:-10718442 MsaT041694.1:CDS
ATCTTATTCTCATGATGACA+AGG 0.605341 8:-10718038 MsaT041694.1:CDS
TGACTGATCATGACAAGCCA+TGG 0.611257 8:+10718189 None:intergenic
AGGAATAAGGGATGGTACTG+TGG 0.625865 8:-10717767 MsaT041694.1:CDS
CAAGTTCAAGGAATAAGGGA+TGG 0.642231 8:-10717775 MsaT041694.1:CDS
GCTGTTGTTGTTGTTCAAGG+AGG 0.650068 8:+10718252 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.653157 8:-10718347 MsaT041694.1:CDS
AAGTAGACCCAGAGCCAGGG+AGG 0.659120 8:-10718459 MsaT041694.1:CDS
GTCAACAAGAACAACAAACA+AGG 0.662924 8:-10717560 MsaT041694.1:CDS
TGTGAAAGACACATGCACAG+AGG 0.772839 8:-10718373 MsaT041694.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 10717262 10718824 10717262 ID=MsaG041694
Chr8 mRNA 10717262 10718824 10717262 ID=MsaT041694.1;Parent=MsaG041694
Chr8 exon 10717262 10717269 10717262 ID=MsaT041694.1.exon4;Parent=MsaT041694.1
Chr8 CDS 10717262 10717269 10717262 ID=cds.MsaT041694.1;Parent=MsaT041694.1
Chr8 exon 10717449 10717801 10717449 ID=MsaT041694.1.exon3;Parent=MsaT041694.1
Chr8 CDS 10717449 10717801 10717449 ID=cds.MsaT041694.1;Parent=MsaT041694.1
Chr8 exon 10718031 10718517 10718031 ID=MsaT041694.1.exon2;Parent=MsaT041694.1
Chr8 CDS 10718031 10718517 10718031 ID=cds.MsaT041694.1;Parent=MsaT041694.1
Chr8 exon 10718641 10718824 10718641 ID=MsaT041694.1.exon1;Parent=MsaT041694.1
Chr8 CDS 10718641 10718824 10718641 ID=cds.MsaT041694.1;Parent=MsaT041694.1
Gene Sequence

>MsaT041694.1

ATGATGAGTGCAAGTTCAAGAAATAGGTCTCTTTTCACACCAAATCAATGGCAAGAACTTGAACAACAAGCCCTAGTTTTTAAATACATGGTTACTGGAACACCTATTCCACCAGATCTCATATACTCTATTAAGAGAAGTTTAGACACTTCAATATCTTCAAGAATCTTTCCTCATCCACCAATTGGGTGGGGATGTTTTGAAATGGGATTTGGCAGAAAAGTAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGCAAGAAATGGAGATGCTCAAAGGAAGCATATCCAGACTCAAAGTACTGTGAAAGACACATGCACAGAGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTAGTTTCTTCTTCAACAACAACACCAACAAATAACACAAACACAGCATCTTCTTACAACAGAAACACCTCCTTGAACAACAACAACAGCAGCATAAACTCACCTTCTTCTTTCCCTTTCTCTACTTCTTCCATGGCTTGTCATGATCAGTCACAATCTTTTTCACAATCCTACCAAAACCCTTACTATTACTCTCAATCAATTACCTCTTCTAAAACACTTGATCATTCTGATTTCCAAACTCAAGATGCTACTACTCATCACCTCTTTTTGGACTCAACATCTTATTCTCATGATGACAAGGACTATAGGAGGCAAGTTCAAGGAATAAGGGATGGTACTGTGGATGAGAGAACTTTCTTTCCAGAAGCTACAGGTTCATCTAGGAGCTGCTATCATGATTCATATCAACAACAACTATCAATGAATCCCTTTAAGTCTTACTCAAGCTCGCAGTTTGAAAATATCAATGATGATAATTCAAGACAACAACAAGAACAACACTGTTTTGTTTTAGGCACTGACATCAAGTCAACAAGAACAACAAACAAGGACAAAGAAAGTGAGACAACTCAGAAACCACTTCATCATTTCTTTGGTGAGTGGACACCAAAGAACACAGATTCCTGGCTAGATCTTGCTTCCAACTCCAGAGTTCCAACAGATGAGTGA

Protein sequence

>MsaT041694.1

MMSASSRNRSLFTPNQWQELEQQALVFKYMVTGTPIPPDLIYSIKRSLDTSISSRIFPHPPIGWGCFEMGFGRKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVELVVSSSTTTPTNNTNTASSYNRNTSLNNNNSSINSPSSFPFSTSSMACHDQSQSFSQSYQNPYYYSQSITSSKTLDHSDFQTQDATTHHLFLDSTSYSHDDKDYRRQVQGIRDGTVDERTFFPEATGSSRSCYHDSYQQQLSMNPFKSYSSSQFENINDDNSRQQQEQHCFVLGTDIKSTRTTNKDKESETTQKPLHHFFGEWTPKNTDSWLDLASNSRVPTDE*