AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041695

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041695 MtrunA17_Chr2g0329031 47.917 192 93 3 33 223 6 191 6.12e-50 173
MsaG041695 MtrunA17_Chr1g0207551 46.114 193 102 1 33 223 26 218 5.71e-49 171
MsaG041695 MtrunA17_Chr3g0080351 39.407 236 100 4 7 222 39 251 5.95e-44 157
MsaG041695 MtrunA17_Chr5g0448761 36.598 194 113 4 37 221 56 248 7.47e-28 110
MsaG041695 MtrunA17_Chr2g0296781 34.239 184 111 3 45 223 1 179 8.99e-23 96.3
MsaG041695 MtrunA17_Chr1g0202341 32.889 225 138 6 2 218 13 232 6.09e-22 93.6
MsaG041695 MtrunA17_Chr7g0233911 41.905 105 56 1 39 138 32 136 2.05e-21 88.2
MsaG041695 MtrunA17_Chr7g0258861 27.895 190 130 3 35 218 4 192 3.69e-20 86.7
MsaG041695 MtrunA17_Chr2g0329361 37.736 106 60 1 40 139 60 165 5.39e-20 85.5
MsaG041695 MtrunA17_Chr2g0287651 29.730 185 113 5 43 215 77 256 1.27e-19 87.0
MsaG041695 MtrunA17_Chr6g0451441 28.708 209 142 4 12 215 53 259 3.42e-19 85.5
MsaG041695 MtrunA17_Chr2g0291591 27.174 184 134 0 35 218 58 241 7.51e-19 84.7
MsaG041695 MtrunA17_Chr2g0276841 29.474 190 119 6 41 217 70 257 5.31e-17 79.3
MsaG041695 MtrunA17_Chr6g0458391 39.048 105 60 1 41 141 225 329 2.39e-16 77.4
MsaG041695 MtrunA17_Chr6g0458391 29.891 184 123 3 41 218 91 274 5.45e-15 73.6
MsaG041695 MtrunA17_Chr4g0033611 35.714 112 67 2 33 140 68 178 4.35e-16 74.7
MsaG041695 MtrunA17_Chr1g0203721 28.351 194 129 4 35 218 54 247 6.61e-16 76.3
MsaG041695 MtrunA17_Chr1g0203051 28.037 214 129 8 8 202 40 247 8.59e-16 75.9
MsaG041695 MtrunA17_Chr3g0078041 34.862 109 66 1 36 139 45 153 2.03e-15 72.4
MsaG041695 MtrunA17_Chr2g0324011 27.778 180 116 5 40 210 9 183 4.40e-15 73.6
MsaG041695 MtrunA17_Chr2g0329421 37.143 105 61 2 40 140 26 129 2.39e-14 69.3
MsaG041695 MtrunA17_Chr2g0291581 28.022 182 121 3 41 222 71 242 7.01e-14 70.1
MsaG041695 MtrunA17_Chr2g0287061 35.514 107 60 1 32 138 174 271 1.24e-13 69.3
MsaG041695 MtrunA17_Chr1g0196541 28.022 182 125 2 45 223 1 179 1.90e-13 68.9
MsaG041695 MtrunA17_Chr3g0082121 30.189 159 101 4 41 191 43 199 2.77e-13 68.6
MsaG041695 MtrunA17_Chr5g0440451 33.094 139 67 5 6 138 13 131 8.02e-13 67.0
MsaG041695 MtrunA17_Chr8g0363841 23.196 194 134 4 33 216 9 197 2.26e-12 65.9
MsaG041695 MtrunA17_Chr5g0441581 25.581 215 132 6 15 223 15 207 7.53e-12 64.3
MsaG041695 MtrunA17_Chr2g0294841 30.000 180 108 6 41 204 45 222 4.94e-11 62.0
MsaG041695 MtrunA17_Chr5g0401121 33.019 106 66 2 41 141 56 161 9.65e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041695 AT4G15090.1 50.265 189 89 2 40 223 50 238 4.50e-54 185
MsaG041695 AT4G15090.5 50.265 189 89 2 40 223 50 238 4.50e-54 185
MsaG041695 AT4G15090.3 50.265 189 89 2 40 223 50 238 4.50e-54 185
MsaG041695 AT4G15090.2 50.265 189 89 2 40 223 50 238 4.50e-54 185
MsaG041695 AT4G15090.4 50.265 189 89 2 40 223 50 238 4.50e-54 185
MsaG041695 AT3G22170.2 41.500 200 78 3 40 222 69 246 3.38e-43 155
MsaG041695 AT3G22170.1 41.500 200 78 3 40 222 69 246 3.38e-43 155
MsaG041695 AT1G76320.1 44.199 181 93 3 45 223 1 175 4.65e-42 151
MsaG041695 AT1G76320.4 44.199 181 93 3 45 223 1 175 4.65e-42 151
MsaG041695 AT1G76320.2 44.199 181 93 3 45 223 1 175 4.65e-42 151
MsaG041695 AT1G76320.3 44.199 181 93 3 45 223 1 175 5.67e-42 150
MsaG041695 AT2G32250.6 53.571 112 49 2 44 154 41 150 6.87e-31 120
MsaG041695 AT2G32250.1 53.571 112 49 2 44 154 41 150 6.87e-31 120
MsaG041695 AT2G32250.3 53.571 112 49 2 44 154 41 150 6.87e-31 120
MsaG041695 AT2G32250.2 53.571 112 49 2 44 154 41 150 7.27e-31 119
MsaG041695 AT2G32250.4 53.571 112 49 2 44 154 41 150 7.27e-31 119
MsaG041695 AT2G32250.5 53.571 112 49 2 44 154 41 150 7.40e-31 119
MsaG041695 AT2G32250.8 53.153 111 49 2 45 154 1 109 5.33e-30 117
MsaG041695 AT2G32250.7 53.153 111 49 2 45 154 1 109 5.87e-30 117
MsaG041695 AT4G38180.1 36.316 190 112 3 40 221 72 260 1.17e-28 113
MsaG041695 AT4G19990.3 40.000 135 64 1 40 157 20 154 3.90e-22 94.7
MsaG041695 AT4G19990.4 40.000 135 64 1 40 157 20 154 3.90e-22 94.7
MsaG041695 AT4G19990.7 40.000 135 64 1 40 157 34 168 4.07e-22 94.7
MsaG041695 AT4G19990.5 40.000 135 64 1 40 157 20 154 4.37e-22 94.4
MsaG041695 AT4G19990.6 40.000 135 64 1 40 157 20 154 5.28e-22 94.4
MsaG041695 AT4G19990.2 40.000 135 64 1 40 157 20 154 5.28e-22 94.4
MsaG041695 AT3G07500.1 38.298 141 70 5 12 140 1 136 1.28e-17 78.6
MsaG041695 AT3G59470.3 32.308 130 84 2 14 140 44 172 8.54e-17 77.0
MsaG041695 AT3G59470.1 32.308 130 84 2 14 140 44 172 8.54e-17 77.0
MsaG041695 AT3G59470.2 32.061 131 85 2 14 141 56 185 9.22e-17 77.0
MsaG041695 AT1G80010.1 31.073 177 114 2 41 209 95 271 3.82e-16 77.4
MsaG041695 AT5G18960.2 32.857 140 83 3 1 138 1 131 1.88e-15 75.1
MsaG041695 AT5G18960.2 36.667 120 67 1 22 141 192 302 1.94e-15 75.1
MsaG041695 AT5G18960.1 32.857 140 83 3 1 138 1 131 1.88e-15 75.1
MsaG041695 AT5G18960.1 36.667 120 67 1 22 141 192 302 1.94e-15 75.1
MsaG041695 AT1G10240.2 27.230 213 137 7 24 223 34 241 1.21e-14 72.8
MsaG041695 AT1G10240.1 27.230 213 137 7 24 223 34 241 1.21e-14 72.8
MsaG041695 AT2G43280.1 33.654 104 64 1 40 138 21 124 1.43e-14 70.1
MsaG041695 AT1G52520.1 32.258 124 83 1 15 138 60 182 5.42e-14 70.9
MsaG041695 AT4G12850.4 35.000 100 64 1 40 138 10 109 8.25e-14 66.6
MsaG041695 AT4G12850.2 35.000 100 64 1 40 138 10 109 1.48e-13 65.9
MsaG041695 AT4G12850.1 35.000 100 64 1 40 138 10 109 1.71e-13 66.6
MsaG041695 AT3G06250.2 28.261 184 96 3 40 222 189 337 2.00e-13 69.3
MsaG041695 AT3G06250.2 34.286 105 60 2 34 138 21 116 4.25e-13 68.2
MsaG041695 AT3G06250.1 28.261 184 96 3 40 222 189 337 2.00e-13 69.3
MsaG041695 AT3G06250.1 34.286 105 60 2 34 138 21 116 4.25e-13 68.2
MsaG041695 AT3G06250.3 28.261 184 96 3 40 222 189 337 2.00e-13 69.3
MsaG041695 AT3G06250.3 34.286 105 60 2 34 138 21 116 4.25e-13 68.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAATTTGTGATGGAAAA+TGG 0.198140 8:-10730666 MsaT041695.1:CDS
AACGCCGACCTTTATCTAAA+TGG 0.253808 8:+10730431 None:intergenic
TATGCTAAGTCAGTTGGATT+TGG 0.280799 8:-10730841 MsaT041695.1:CDS
TTCATGTACCGTTGATTTAG+AGG 0.293148 8:-10730938 MsaT041695.1:CDS
CGGCGTTTGGCTTTAGAATC+AGG 0.298007 8:-10730415 MsaT041695.1:CDS
GAGCTTTATTGATTCCTCTA+TGG 0.299611 8:+10730575 None:intergenic
TCAAATTCCATTCCAATGTT+AGG 0.302097 8:+10730898 None:intergenic
GCCACAATGGACAAAGAATA+TGG 0.322237 8:-10730496 MsaT041695.1:CDS
ATGCTAAGTCAGTTGGATTT+GGG 0.354359 8:-10730840 MsaT041695.1:CDS
GTAAGCAACCATTTAGATAA+AGG 0.361819 8:-10730439 MsaT041695.1:CDS
TTTAGATAAAGGTCGGCGTT+TGG 0.420224 8:-10730428 MsaT041695.1:CDS
GAATTAATGGAAGTTGTTGA+TGG 0.438521 8:-10731024 None:intergenic
GGAAAATAATGTGCATATGC+TGG 0.447627 8:+10730601 None:intergenic
CGTGTTAAAAGAATTTGTGA+TGG 0.498075 8:-10730673 MsaT041695.1:CDS
ACTCATTATGCTAAGTCAGT+TGG 0.500199 8:-10730847 MsaT041695.1:CDS
TCCATATTCTTTGTCCATTG+TGG 0.503057 8:+10730495 None:intergenic
AATTAATGGAAGTTGTTGAT+GGG 0.514194 8:-10731023 None:intergenic
AGTTATGCTTGCACAAGATA+CGG 0.514711 8:-10730763 MsaT041695.1:CDS
ATGGAAGTTGTTGATGGGGA+TGG 0.521174 8:-10731018 MsaT041695.1:CDS
AAATCCACGTCCTTGTTTGA+AGG 0.523815 8:-10730716 MsaT041695.1:CDS
ATTGAAACTTTGCACCATGT+TGG 0.528985 8:-10730541 MsaT041695.1:CDS
TCCACGTCCTTGTTTGAAGG+TGG 0.544839 8:-10730713 MsaT041695.1:CDS
AGGAGATGCAAATACAATGT+TGG 0.548389 8:-10730395 MsaT041695.1:CDS
TTATTGATTCCTCTATGGCA+AGG 0.554774 8:+10730580 None:intergenic
CCACGTCCTTGTTTGAAGGT+GGG 0.564321 8:-10730712 MsaT041695.1:CDS
GAATGAAGAGCAAGCTGTCA+AGG 0.567338 8:-10730977 MsaT041695.1:CDS
TCAAGAACCTAACATTGGAA+TGG 0.572602 8:-10730905 MsaT041695.1:CDS
TGCTTGTTCTCACTCCAACA+TGG 0.579133 8:+10730527 None:intergenic
TTGTTTGAAGGTGGGATGTG+AGG 0.585943 8:-10730704 MsaT041695.1:CDS
CCCACCTTCAAACAAGGACG+TGG 0.589716 8:+10730712 None:intergenic
TCACATCCCACCTTCAAACA+AGG 0.596792 8:+10730706 None:intergenic
GGTGGTCAAGAACCTAACAT+TGG 0.606305 8:-10730910 MsaT041695.1:CDS
ACCGTTAGCCTCTAAATCAA+CGG 0.614838 8:+10730930 None:intergenic
ACCGTTGATTTAGAGGCTAA+CGG 0.628314 8:-10730931 MsaT041695.1:CDS
ATTAATGGAAGTTGTTGATG+GGG 0.631609 8:-10731022 None:intergenic
GCAACCATTTAGATAAAGGT+CGG 0.637277 8:-10730435 MsaT041695.1:CDS
ACAATGGACAAAGAATATGG+AGG 0.732815 8:-10730493 MsaT041695.1:CDS
GTTGATTTAGAGGCTAACGG+TGG 0.762259 8:-10730928 MsaT041695.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 10730369 10731040 10730369 ID=MsaG041695
Chr8 mRNA 10730369 10731040 10730369 ID=MsaT041695.1;Parent=MsaG041695
Chr8 exon 10730369 10731040 10730369 ID=MsaT041695.1.exon1;Parent=MsaT041695.1
Chr8 CDS 10730369 10731040 10730369 ID=cds.MsaT041695.1;Parent=MsaT041695.1
Gene Sequence

>MsaT041695.1

ATGGAAGTTGTTGATGGGGATGGTGAACATTTTATTGATGGGAATGAAGAGCAAGCTGTCAAGGAGATTGATGACAATGATTCATGTACCGTTGATTTAGAGGCTAACGGTGGTCAAGAACCTAACATTGGAATGGAATTTGACTCACAAGAAAATGCTTATTCATTCTACACTCATTATGCTAAGTCAGTTGGATTTGGGATATCAATTAAAAATAGTCGTCGTTCAAAGATTTTTAGAGAGTTAATTGATGTAAGTTATGCTTGCACAAGATACGGCAAGAAGCGAGAATCCAGTTCACAAAATCCACGTCCTTGTTTGAAGGTGGGATGTGAGGCTAGTTTACGTGTTAAAAGAATTTGTGATGGAAAATGGATTGTTCATAGCTTCGTTAAAGACCACAATCACGAACTTTTTCCAGCATATGCACATTATTTTCCTTGCCATAGAGGAATCAATAAAGCTCAAAAGCATTCTATTGAAACTTTGCACCATGTTGGAGTGAGAACAAGCAAATTTTTTGCCACAATGGACAAAGAATATGGAGGATATAAAAACATTGGTTGCTTGGAGAAAGATGTAAGCAACCATTTAGATAAAGGTCGGCGTTTGGCTTTAGAATCAGGAGATGCAAATACAATGTTGGATTGTTTTATGCTCATGCAAAAGTAG
Protein sequence

>MsaT041695.1

MEVVDGDGEHFIDGNEEQAVKEIDDNDSCTVDLEANGGQEPNIGMEFDSQENAYSFYTHYAKSVGFGISIKNSRRSKIFRELIDVSYACTRYGKKRESSSQNPRPCLKVGCEASLRVKRICDGKWIVHSFVKDHNHELFPAYAHYFPCHRGINKAQKHSIETLHHVGVRTSKFFATMDKEYGGYKNIGCLEKDVSNHLDKGRRLALESGDANTMLDCFMLMQK*