AlfalfaGEDB | MsaG041749

M. sativa ssp. caerulea / MsaG041749

KEGG: -
GO: -
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041749	KEH18496.1	80.228	526	50	6	1	474	1	524	0.0	756
MsaG041749	KEH18496.1	90.751	173	12	1	431	603	559	727	1.11e-94	316

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041749	sp\|Q9M1L7\|PRK3_ARATH	37.888	483	219	12	13	446	5	455	3.28e-94	305
MsaG041749	sp\|Q9M1L7\|PRK3_ARATH	48.980	147	73	1	398	542	485	631	6.63e-38	152

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041749	tr\|A0A072TMR8\|A0A072TMR8_MEDTR	80.228	526	50	6	1	474	1	524	0.0	756
MsaG041749	tr\|A0A072TMR8\|A0A072TMR8_MEDTR	90.751	173	12	1	431	603	559	727	5.31e-95	316

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041749	MtrunA17_Chr8g0344471	80.228	526	50	6	1	474	1	524	0.0	756
MsaG041749	MtrunA17_Chr8g0344471	90.751	173	12	1	431	603	559	727	1.02e-98	316
MsaG041749	MtrunA17_Chr2g0301071	45.598	443	179	10	29	436	26	441	3.67e-124	374
MsaG041749	MtrunA17_Chr4g0070291	44.541	458	212	10	14	445	6	447	2.56e-119	367
MsaG041749	MtrunA17_Chr4g0070291	49.697	165	66	4	390	544	470	627	1.19e-40	156
MsaG041749	MtrunA17_Chr8g0379571	33.756	631	304	21	8	543	45	656	1.89e-82	272
MsaG041749	MtrunA17_Chr1g0147701	37.787	479	256	8	15	474	12	467	4.63e-81	268
MsaG041749	MtrunA17_Chr1g0147701	41.441	111	64	1	436	545	507	617	1.09e-21	99.8
MsaG041749	MtrunA17_Chr8g0388991	36.607	448	232	10	14	440	17	433	5.89e-80	264
MsaG041749	MtrunA17_Chr8g0388991	41.284	109	63	1	436	543	509	617	1.10e-21	99.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041749	AT1G72460.1	41.834	447	226	7	16	436	6	444	1.49e-95	306
MsaG041749	AT1G72460.1	39.474	114	64	2	431	539	517	630	1.07e-17	87.4
MsaG041749	AT3G42880.1	37.888	483	219	12	13	446	5	455	3.33e-95	305
MsaG041749	AT3G42880.1	48.980	147	73	1	398	542	485	631	6.74e-39	152
MsaG041749	AT5G20690.1	39.186	467	221	15	30	446	28	481	3.81e-91	295
MsaG041749	AT5G20690.1	52.349	149	69	1	398	544	511	659	2.25e-41	159
MsaG041749	AT5G35390.1	37.582	455	223	10	20	440	21	448	4.22e-81	268
MsaG041749	AT5G35390.1	36.842	114	70	1	435	548	523	634	7.07e-16	81.6
MsaG041749	AT2G07040.1	37.639	449	231	11	16	440	6	429	6.43e-79	262
MsaG041749	AT2G07040.1	32.456	114	76	1	430	542	499	612	3.49e-13	73.2

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Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAATTCGATTCAACTC+TGG	0.582496	8:+11337970	None:intergenic
AAAGCATCTGCTGAAGTTCT+TGG	0.298349	8:-11337142	MsaT041749.1:CDS
AAATAGAGTAGACTTCCTCT+AGG	0.581660	8:+11336911	None:intergenic
AAATTTGAATAATCCTTCAT+TGG	0.395904	8:-11337752	MsaT041749.1:CDS
AACATTACTAGCAGTATTGT+TGG	0.407868	8:+11337534	None:intergenic
AAGATCAAGATGAAGAATGG+TGG	0.612930	8:+11338282	None:intergenic
AATAATGAGAAGGGTGTGTT+TGG	0.454488	8:-11337181	MsaT041749.1:CDS
AATGAAGGATTATTCAAATT+TGG	0.144111	8:+11337754	None:intergenic
AATGGTGGATGGAAAATTGC+AGG	0.436576	8:+11338297	None:intergenic
ACATCAAATATGACCAATGA+AGG	0.485479	8:+11337739	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	11335679	11338334	11335679	ID=MsaG041749
Chr8	mRNA	11335679	11338334	11335679	ID=MsaT041749.1;Parent=MsaG041749
Chr8	exon	11335679	11336184	11335679	ID=MsaT041749.1.exon4;Parent=MsaT041749.1
Chr8	CDS	11335679	11336184	11335679	ID=cds.MsaT041749.1;Parent=MsaT041749.1
Chr8	exon	11336903	11337292	11336903	ID=MsaT041749.1.exon3;Parent=MsaT041749.1
Chr8	CDS	11336903	11337292	11336903	ID=cds.MsaT041749.1;Parent=MsaT041749.1
Chr8	exon	11337386	11337670	11337386	ID=MsaT041749.1.exon2;Parent=MsaT041749.1
Chr8	CDS	11337386	11337670	11337386	ID=cds.MsaT041749.1;Parent=MsaT041749.1
Chr8	exon	11337704	11338334	11337704	ID=MsaT041749.1.exon1;Parent=MsaT041749.1
Chr8	CDS	11337704	11338334	11337704	ID=cds.MsaT041749.1;Parent=MsaT041749.1

Gene Sequence

>MsaT041749.1

ATGGCCATAGCAGCTCCTGCAATTTTCCATCCACCATTCTTCATCTTGATCTTCAACATTATCATAAATTTTTCAATGATATATTCTATGTCAGAAACAGAAGCTTTATTAAGCCTAAAAAACTCATTCTCAAATGCTAATGCTCTAAATAGTTGGATTGCCAACACTTTACCTTGTACCGAAGACGATCAATGGGAAGGCGTTGTTTGTTACAACGGTCTTGTAACCGGTCTGCGCCTTGAAGGAATGGGATTATTTGGCAAAATTGATGTTGATGCATTGCTTGAACTTAAAGGTTTAAGAACTATTAGTCTTATGAATAATTCTTTCACTGGTCCGATACCAGAGTTGAATCGAATTGGTTTCTTGAAAGCGATGTTTTTGTCCGGAAACAAATTTTCAGGACATATTCCTAAGGAATATTTTCAAAGAATGAAATCATTGAAGAAAGTTTGGTTATCTAATAATGAATTTACTGGTGACATTCCATCATCATTGGCTGAGATTCCTCAACTTGTTGAATTACATCTTGAAAAAAATCAGTTTAGTGGTAATATTCCAAATTTGAATAATCCTTCATTGGTCATATTTGATGTTTCAAACAATAATTTGGAAGGTGAAGTTCCTCAAGGAAATTCTGGTTTATGTGTAAAAATCGGTAAAATATGTGGACAACAACCAGTGCAACAAACAAATCCTAATCCTATTGATGCACATAATATTAGTATTAGTAATACATCAGTTACCAACAATACTGCTAGTAATGTTATTGTGACTGTGCCTGATAACAGTAAGCATAAGTCATTGCAGATTATTGGTATTGTTATAACAAGTGTAGTAATAATTTCATTAGCTATATTTTTGATTATCAAATCAAGAAAAAAGAAAAAGGAGGGTAAATTTGATGATATTATTGAGAAAAGAGATATGAATTTTGTGAAAAAATCATCTAGTAAAAGAGGTTCATCTCAAGGTAGTAAAGGGATAGGTGAGCTTGTGATGATGAATAATGAGAAGGGTGTGTTTGGTTTACCTGATTTGATGAAAGCATCTGCTGAAGTTCTTGGAAATGGAGGGTTTGGATCATCTTATAAAGCTGTGATGGCTAATGGTGTTGCTGTGGTTGTGAAAAGGACTAGAGAATTGAATGCTTTGGGAAAAGATGGTTTTGATGCTGAGATGAAAAAGCTTGGAAGGTTAAAACATTGGAATGTTTTGACACCTTTGGCTTATCATTACAGAAAAGATGAGAAATTAGTCATCTCTGAGTATGTTCCTAGAGGAAGTCTACTCTATTTGCTGCATGCACCAGAAGCATTACAACACAACCAAATTTCACCTCGTTGTGACGTATACTGTCTCGGTGTAGTCATACTCGAAATACTAACCGGAAAATTTCCTTCTCAATATCTTAGCAACGGTAAAGGCGGAACCGATGTAGTACAATGGGTAGCATCAGCAATTTCTGAAGGAAGAGAAGTTGAACTACTTGATCCTGAAATTGCAAGTAATAAAAATTCATTAGGTGAAATGAAACAACTTGTTCATATTGGTGCTGCTTGTACTGAAAGTAATCCTCAAAACAGATTAGATATGATGGAAGCAGTTAGAAGAATAGAAGAGATTAAAACAAATGGTGTTGATCAAGTTCAAGAATCAAGAACAATAGAGGTTTTACCTTCTCTTAGCGATGGTTTTGGTGATCCTTCTCAAGCATATGATAATCAAGGGCATGTTGGTGATCAATCAAGTAAGAGACATGGAACAAGTAGTTTTGGTAGTAAGGATAATTTTGAATTTGGTATCTCCTAA

Protein sequence

>MsaT041749.1

MAIAAPAIFHPPFFILIFNIIINFSMIYSMSETEALLSLKNSFSNANALNSWIANTLPCTEDDQWEGVVCYNGLVTGLRLEGMGLFGKIDVDALLELKGLRTISLMNNSFTGPIPELNRIGFLKAMFLSGNKFSGHIPKEYFQRMKSLKKVWLSNNEFTGDIPSSLAEIPQLVELHLEKNQFSGNIPNLNNPSLVIFDVSNNNLEGEVPQGNSGLCVKIGKICGQQPVQQTNPNPIDAHNISISNTSVTNNTASNVIVTVPDNSKHKSLQIIGIVITSVVIISLAIFLIIKSRKKKKEGKFDDIIEKRDMNFVKKSSSKRGSSQGSKGIGELVMMNNEKGVFGLPDLMKASAEVLGNGGFGSSYKAVMANGVAVVVKRTRELNALGKDGFDAEMKKLGRLKHWNVLTPLAYHYRKDEKLVISEYVPRGSLLYLLHAPEALQHNQISPRCDVYCLGVVILEILTGKFPSQYLSNGKGGTDVVQWVASAISEGREVELLDPEIASNKNSLGEMKQLVHIGAACTESNPQNRLDMMEAVRRIEEIKTNGVDQVQESRTIEVLPSLSDGFGDPSQAYDNQGHVGDQSSKRHGTSSFGSKDNFEFGIS*