AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041766 MtrunA17_Chr8g0344681 95.166 331 12 2 1 330 1 328 0.0 553
MsaG041766 MtrunA17_Chr2g0283871 59.804 102 37 2 66 166 19 117 1.01e-35 133
MsaG041766 MtrunA17_Chr2g0283871 53.030 66 28 1 265 330 236 298 8.28e-15 74.7
MsaG041766 MtrunA17_Chr4g0041151 57.391 115 35 4 65 171 26 134 8.35e-34 127
MsaG041766 MtrunA17_Chr2g0331931 30.418 263 131 8 70 330 274 486 3.73e-22 98.2
MsaG041766 MtrunA17_Chr2g0325841 39.450 109 58 3 67 171 43 147 2.64e-17 84.3
MsaG041766 MtrunA17_Chr3g0082261 26.299 308 162 12 54 330 21 294 4.14e-17 81.6
MsaG041766 MtrunA17_Chr6g0485241 36.000 100 59 2 70 169 130 224 2.09e-16 80.1
MsaG041766 MtrunA17_Chr1g0164461 36.066 122 68 2 54 174 21 133 6.10e-15 72.8
MsaG041766 MtrunA17_Chr1g0179531 34.375 96 57 2 70 165 133 222 9.25e-15 75.9
MsaG041766 MtrunA17_Chr4g0066271 32.632 95 62 1 69 163 116 208 1.84e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041766 AT3G14180.1 47.396 384 110 11 34 330 24 402 2.58e-89 277
MsaG041766 AT1G54060.1 41.176 374 147 13 1 330 1 345 6.74e-53 181
MsaG041766 AT5G05550.3 39.552 268 102 7 65 330 19 228 1.74e-43 152
MsaG041766 AT5G05550.1 39.552 268 102 7 65 330 19 228 2.34e-43 151
MsaG041766 AT5G05550.2 39.552 268 102 7 65 330 19 228 2.79e-43 151
MsaG041766 AT3G58630.2 37.143 280 146 8 66 329 22 287 4.88e-43 152
MsaG041766 AT3G58630.1 37.143 280 146 8 66 329 22 287 6.09e-43 152
MsaG041766 AT3G11100.1 41.852 270 98 7 65 330 17 231 7.44e-43 150
MsaG041766 AT3G10030.2 36.283 113 66 2 70 182 161 267 8.25e-18 85.5
MsaG041766 AT3G10030.1 36.283 113 66 2 70 182 161 267 1.07e-17 85.1
MsaG041766 AT3G24490.1 33.846 130 76 4 70 197 90 211 7.79e-17 81.3
MsaG041766 AT3G54390.1 40.404 99 50 2 66 164 34 123 1.33e-16 80.1
MsaG041766 AT3G54390.2 40.404 99 50 2 66 164 50 139 1.50e-16 80.1
MsaG041766 AT2G44730.1 32.632 95 62 1 69 163 63 155 3.49e-12 67.8
MsaG041766 AT3G24860.1 28.800 125 67 3 39 163 45 147 1.02e-11 65.9

Find 111 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCAGGTACGGCTGCAGTTT+GGG 0.185680 8:+11568012 None:intergenic
ACTCAGGCAATTGTGAAATT+TGG 0.238839 8:-11569376 MsaT041766.1:CDS
ATAGCTATGGGACTGAATTT+TGG 0.267179 8:+11568093 None:intergenic
GATGAAGTTTGCTAAGGATT+TGG 0.272524 8:-11569290 MsaT041766.1:CDS
GATCGGTTAGATCAGTTAAT+TGG 0.279898 8:-11569748 MsaT041766.1:CDS
GCCAAGATTAGTGGTGTTTC+CGG 0.285327 8:-11569718 MsaT041766.1:CDS
AGGCAGGTACGGCTGCAGTT+TGG 0.318815 8:+11568011 None:intergenic
TTCGGTGACGGTTGCGGCAC+CGG 0.338241 8:-11570112 MsaT041766.1:CDS
GAGGTTGCGGAGATTGTTAA+TGG 0.341472 8:-11569919 MsaT041766.1:CDS
TCCATCTCCGGTGTCGTCTC+CGG 0.352746 8:-11570151 MsaT041766.1:CDS
AAATTCGAAGGGGAGAGGTT+GGG 0.373866 8:-11569419 MsaT041766.1:CDS
CCTGTTGGTATTCGCGCCTC+TGG 0.397120 8:-11569640 MsaT041766.1:CDS
GTTTCACGTCCCAGCGGTGC+TGG 0.399693 8:+11567924 None:intergenic
GTGGAGGGAGGGAGGATTGT+TGG 0.405449 8:-11570032 MsaT041766.1:CDS
CCATCTCCGGTGTCGTCTCC+GGG 0.410853 8:-11570150 MsaT041766.1:CDS
AGCGTGGGGAGAGAGGTATT+TGG 0.415050 8:-11569980 MsaT041766.1:CDS
AAAACAGCCGCAACAGCTGC+TGG 0.415089 8:-11567987 MsaT041766.1:CDS
AATTCGAAGGGGAGAGGTTG+GGG 0.424508 8:-11569418 MsaT041766.1:CDS
GGGAGCGATCGGTAGGATTT+CGG 0.429065 8:-11570130 MsaT041766.1:CDS
ACCGGAAACACCACTAATCT+TGG 0.441082 8:+11569717 None:intergenic
GGCGGTGTTGATTGAAGCGT+GGG 0.449332 8:-11569995 MsaT041766.1:CDS
AATGCATAGCTGTTCGCCGC+CGG 0.453869 8:+11570093 None:intergenic
GAGGTTGGGGAAGTGCGGTG+AGG 0.461820 8:-11569405 MsaT041766.1:CDS
AGGTTGGGGAAGTGCGGTGA+GGG 0.465619 8:-11569404 MsaT041766.1:CDS
ATCATAAAACCGCCAAGTGC+TGG 0.468333 8:+11569768 None:intergenic
GTTTCCGGTACCAGTCATAC+TGG 0.474144 8:-11569703 MsaT041766.1:CDS
ATCGGTGCTGAAGGTGGCTC+CGG 0.475507 8:-11569802 MsaT041766.1:CDS
TAGGATTTCGGTGACGGTTG+CGG 0.476805 8:-11570118 MsaT041766.1:CDS
GTCGAAGTTGCAGCAGGTGG+TGG 0.476922 8:-11569329 MsaT041766.1:CDS
CAAATTCGAAGGGGAGAGGT+TGG 0.478124 8:-11569420 MsaT041766.1:CDS
GCGGAGATTGTTAATGGAAG+AGG 0.480346 8:-11569913 MsaT041766.1:CDS
CTGATATTCAGTGTAAGAAT+CGG 0.482219 8:-11569867 MsaT041766.1:CDS
GGTCCAGCTGCCAAGATTAG+TGG 0.484802 8:-11569727 MsaT041766.1:CDS
CCAGAGGCGCGAATACCAAC+AGG 0.485804 8:+11569640 None:intergenic
GGCGAGTGGTTCTCCATCTC+CGG 0.489845 8:-11570163 MsaT041766.1:CDS
GGTTAAGAAGAAGTATAAAT+CGG 0.490848 8:-11569836 MsaT041766.1:CDS
GGAATTTGAAGCAGAAGCAT+TGG 0.493242 8:-11569945 MsaT041766.1:CDS
ACCTTCGCTCTCTTCCTCTC+CGG 0.494047 8:+11569451 None:intergenic
GTTGCAGCAGGTGGTGGAGA+TGG 0.496264 8:-11569323 MsaT041766.1:CDS
GCTCTCTTCCTCTCCGGTGG+TGG 0.502946 8:+11569457 None:intergenic
CCACGTGTCGTATTCTGAAT+CGG 0.509196 8:+11570063 None:intergenic
TGGAAGCAACGGTGGAGGGA+GGG 0.519025 8:-11570043 MsaT041766.1:CDS
GTGTCGTCTCCGGGAGCGAT+CGG 0.520173 8:-11570141 MsaT041766.1:CDS
GGTATTTGGAATTGAGCAGA+GGG 0.520971 8:-11569966 MsaT041766.1:CDS
GAAGCATTGGAAAGAGGTTG+CGG 0.521472 8:-11569932 MsaT041766.1:CDS
GATCGGTAGGATTTCGGTGA+CGG 0.523152 8:-11570124 MsaT041766.1:CDS
GGAGAACCACTCGCCGGAGA+TGG 0.523745 8:+11570171 None:intergenic
GAAGCAGAAGCATTGGAAAG+AGG 0.525294 8:-11569938 MsaT041766.1:CDS
AATTCAAATTCGAAGGGGAG+AGG 0.527517 8:-11569424 MsaT041766.1:CDS
CCCGGAGACGACACCGGAGA+TGG 0.529470 8:+11570150 None:intergenic
TCACAGCAGAAAGTTCCGTT+AGG 0.530119 8:-11569667 MsaT041766.1:CDS
GCAGAGGATGAAGTTTGCTA+AGG 0.530659 8:-11569296 MsaT041766.1:CDS
GGTGATGAATTCAAATTCGA+AGG 0.540299 8:-11569431 MsaT041766.1:CDS
ACACCACTAATCTTGGCAGC+TGG 0.541940 8:+11569724 None:intergenic
GAAATCCTACCGATCGCTCC+CGG 0.544759 8:+11570132 None:intergenic
GGATGCTTGAGGCATAGCTA+TGG 0.545553 8:+11568080 None:intergenic
GTGGAAGCAACGGTGGAGGG+AGG 0.547610 8:-11570044 MsaT041766.1:CDS
CACGTGGAAGCAACGGTGGA+GGG 0.548507 8:-11570047 MsaT041766.1:CDS
GTGTAAGAATCGGATTGATA+CGG 0.548630 8:-11569857 MsaT041766.1:CDS
ATGACTGTTTCCAGTATGAC+TGG 0.550475 8:+11569693 None:intergenic
GGAGACGTCGAAGTTGCAGC+AGG 0.559673 8:-11569335 MsaT041766.1:CDS
TGCGGTGAGGGAATTGACTC+AGG 0.559802 8:-11569392 MsaT041766.1:CDS
GCAGGTACGGCTGCAGTTTG+GGG 0.560060 8:+11568013 None:intergenic
TGATAGTCCAGCAGCTGTTG+CGG 0.560612 8:+11567980 None:intergenic
AATACGACACGTGGAAGCAA+CGG 0.563694 8:-11570054 MsaT041766.1:CDS
AGGTATTTGGAATTGAGCAG+AGG 0.565655 8:-11569967 MsaT041766.1:CDS
TGGTGGAGATGGAGAAGCAG+AGG 0.566345 8:-11569312 MsaT041766.1:CDS
TGATTGAAGCGTGGGGAGAG+AGG 0.567107 8:-11569987 MsaT041766.1:CDS
GGGGAGAGGTTGGGGAAGTG+CGG 0.570094 8:-11569410 MsaT041766.1:CDS
GTGTTACAACCAGCACTTGG+CGG 0.570584 8:-11569777 MsaT041766.1:CDS
CGGCGGTGTTGATTGAAGCG+TGG 0.570892 8:-11569996 MsaT041766.1:CDS
GACGTCGAAGTTGCAGCAGG+TGG 0.576057 8:-11569332 MsaT041766.1:CDS
AAAGCTAAGATCGGTGCTGA+AGG 0.576418 8:-11569811 MsaT041766.1:CDS
GGTGCTGAAGGTGGCTCCGG+CGG 0.576938 8:-11569799 MsaT041766.1:CDS
ACACGTGGAAGCAACGGTGG+AGG 0.578608 8:-11570048 MsaT041766.1:CDS
GTTTCCAGTATGACTGGTAC+CGG 0.580255 8:+11569699 None:intergenic
GTGATGAATTCAAATTCGAA+GGG 0.583321 8:-11569430 MsaT041766.1:CDS
ATCGCTCCCGGAGACGACAC+CGG 0.585960 8:+11570144 None:intergenic
TTCGCTCTCTTCCTCTCCGG+TGG 0.586645 8:+11569454 None:intergenic
GCTATTGCTTGGGATGCTTG+AGG 0.588655 8:+11568069 None:intergenic
AGTGCTGGTTGTAACACCGC+CGG 0.591035 8:+11569783 None:intergenic
GCGGTGTTACAACCAGCACT+TGG 0.592137 8:-11569780 MsaT041766.1:CDS
TTGTTGGAGCGAAGCAGCGA+CGG 0.593492 8:-11570016 MsaT041766.1:CDS
AAGCGACAGTTTACCACCAC+CGG 0.595248 8:-11569470 MsaT041766.1:CDS
AAGCAACGGTGGAGGGAGGG+AGG 0.596019 8:-11570040 MsaT041766.1:CDS
GATCCACTCGAATCCATCTC+CGG 0.596077 8:-11570184 MsaT041766.1:CDS
GATGCTTGAGGCATAGCTAT+GGG 0.599077 8:+11568081 None:intergenic
CGTCTCCGGGAGCGATCGGT+AGG 0.601980 8:-11570137 MsaT041766.1:CDS
GCTAAGATCGGTGCTGAAGG+TGG 0.602432 8:-11569808 MsaT041766.1:CDS
ACCGGAGAGGAAGAGAGCGA+AGG 0.606990 8:-11569452 MsaT041766.1:CDS
ATTCGCGCCTCTGGAGCTAG+AGG 0.614912 8:-11569631 MsaT041766.1:CDS
TGGTGAAGCTTATGAACAAG+CGG 0.615901 8:-11569356 MsaT041766.1:CDS
TCGAATCCATCTCCGGCGAG+TGG 0.616080 8:-11570177 MsaT041766.1:CDS
AAATCGGAGAAAGCTAAGAT+CGG 0.619321 8:-11569820 MsaT041766.1:CDS
GGTGACGGTTGCGGCACCGG+CGG 0.620431 8:-11570109 MsaT041766.1:CDS
GTTCCGTTAGGAATTCCTGT+TGG 0.621548 8:-11569655 MsaT041766.1:CDS
TTGGAGCGAAGCAGCGACGG+CGG 0.631965 8:-11570013 MsaT041766.1:CDS
GGAGATGGAGAACCACTCGC+CGG 0.634434 8:+11570165 None:intergenic
GGTAAACTGTCGCTTGACAC+CGG 0.634602 8:+11569478 None:intergenic
ATTCGCGCCTCTAGCTCCAG+AGG 0.645061 8:+11569624 None:intergenic
CTGCCTGTTTCACGTCCCAG+CGG 0.645719 8:+11567918 None:intergenic
ACGACACGTGGAAGCAACGG+TGG 0.649070 8:-11570051 MsaT041766.1:CDS
CCGATTCAGAATACGACACG+TGG 0.663489 8:-11570063 MsaT041766.1:CDS
ATACCAACAGGAATTCCTAA+CGG 0.670478 8:+11569652 None:intergenic
TCGCCGGAGATGGATTCGAG+TGG 0.676446 8:+11570181 None:intergenic
ACAGTTTACCACCACCGGAG+AGG 0.677094 8:-11569465 MsaT041766.1:CDS
TGATGAATTCAAATTCGAAG+GGG 0.682099 8:-11569429 MsaT041766.1:CDS
TGACGACGACGAGATCCACT+CGG 0.685256 8:-11570214 MsaT041766.1:CDS
GAATTCAGCTCAGAAACGCG+TGG 0.694751 8:+11569535 None:intergenic
GCGGTGTTGATTGAAGCGTG+GGG 0.735042 8:-11569994 MsaT041766.1:CDS
AGTGGATCGAGTTAACCGAG+TGG 0.771483 8:+11570199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 11567927 11570241 11567927 ID=MsaG041766
Chr8 mRNA 11567927 11570241 11567927 ID=MsaT041766.1;Parent=MsaG041766
Chr8 exon 11567927 11568124 11567927 ID=MsaT041766.1.exon2;Parent=MsaT041766.1
Chr8 CDS 11567927 11568124 11567927 ID=cds.MsaT041766.1;Parent=MsaT041766.1
Chr8 exon 11569255 11570241 11569255 ID=MsaT041766.1.exon1;Parent=MsaT041766.1
Chr8 CDS 11569255 11570241 11569255 ID=cds.MsaT041766.1;Parent=MsaT041766.1
Gene Sequence

>MsaT041766.1

ATGGATGACGACGACGAGATCCACTCGGTTAACTCGATCCACTCGAATCCATCTCCGGCGAGTGGTTCTCCATCTCCGGTGTCGTCTCCGGGAGCGATCGGTAGGATTTCGGTGACGGTTGCGGCACCGGCGGCGAACAGCTATGCATTAGCGCTTCCGATTCAGAATACGACACGTGGAAGCAACGGTGGAGGGAGGGAGGATTGTTGGAGCGAAGCAGCGACGGCGGTGTTGATTGAAGCGTGGGGAGAGAGGTATTTGGAATTGAGCAGAGGGAATTTGAAGCAGAAGCATTGGAAAGAGGTTGCGGAGATTGTTAATGGAAGAGGTGATTATTTGAAAGCTCCGAAAACTGATATTCAGTGTAAGAATCGGATTGATACGGTTAAGAAGAAGTATAAATCGGAGAAAGCTAAGATCGGTGCTGAAGGTGGCTCCGGCGGTGTTACAACCAGCACTTGGCGGTTTTATGATCGGTTAGATCAGTTAATTGGTCCAGCTGCCAAGATTAGTGGTGTTTCCGGTACCAGTCATACTGGAAACAGTCATTTGTCACAGCAGAAAGTTCCGTTAGGAATTCCTGTTGGTATTCGCGCCTCTGGAGCTAGAGGCGCGAATCAAGCTAATTCTCAGAAGAAAGATATTCAACAAGCTCAGTTGAATTATCAGAAGATTCAGCTCAGACCACGCGTTTCTGAGCTGAATTCTTCTGATAATTCATCTGAAAAAGAAGCGTTGTCACCGGTGTCAAGCGACAGTTTACCACCACCGGAGAGGAAGAGAGCGAAGGTGATGAATTCAAATTCGAAGGGGAGAGGTTGGGGAAGTGCGGTGAGGGAATTGACTCAGGCAATTGTGAAATTTGGTGAAGCTTATGAACAAGCGGAGACGTCGAAGTTGCAGCAGGTGGTGGAGATGGAGAAGCAGAGGATGAAGTTTGCTAAGGATTTGGAGTTGCAGAGAATGCAGTTTTTCATGAAAACTCAGTGCGGACCACCAAAATTCAGTCCCATAGCTATGCCTCAAGCATCCCAAGCAATAGCCACCAAAAACCGACCGAGGATAAGGAAAAAACACCCCAAACTGCAGCCGTACCTGCCTGAAAACAGCCGCAACAGCTGCTGGACTATCATGACAGCAGCACAGCAGCGCTGCCACAGAAAAACCAGCACCGCTGGGACGTGA

Protein sequence

>MsaT041766.1

MDDDDEIHSVNSIHSNPSPASGSPSPVSSPGAIGRISVTVAAPAANSYALALPIQNTTRGSNGGGREDCWSEAATAVLIEAWGERYLELSRGNLKQKHWKEVAEIVNGRGDYLKAPKTDIQCKNRIDTVKKKYKSEKAKIGAEGGSGGVTTSTWRFYDRLDQLIGPAAKISGVSGTSHTGNSHLSQQKVPLGIPVGIRASGARGANQANSQKKDIQQAQLNYQKIQLRPRVSELNSSDNSSEKEALSPVSSDSLPPPERKRAKVMNSNSKGRGWGSAVRELTQAIVKFGEAYEQAETSKLQQVVEMEKQRMKFAKDLELQRMQFFMKTQCGPPKFSPIAMPQASQAIATKNRPRIRKKHPKLQPYLPENSRNSCWTIMTAAQQRCHRKTSTAGT*