Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG041808 | XP_013450398.1 | 88.991 | 327 | 31 | 2 | 1 | 322 | 1 | 327 | 0.0 | 583 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG041808 | sp|Q8GX84|C3H1_ARATH | 68.790 | 314 | 92 | 4 | 13 | 322 | 25 | 336 | 4.37e-157 | 446 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG041808 | tr|A0A072U502|A0A072U502_MEDTR | 88.991 | 327 | 31 | 2 | 1 | 322 | 1 | 327 | 0.0 | 583 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG041808 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG021131 | MsaG041808 | 0.801507 | 1.460743e-48 | 2.043409e-46 |
| MsaG021165 | MsaG041808 | 0.803775 | 4.999363e-49 | 7.367157e-47 |
| MsaG028632 | MsaG041808 | 0.806226 | 1.544690e-49 | 2.410094e-47 |
| MsaG041808 | MsaG041812 | 0.816483 | 9.382752e-52 | 1.883326e-49 |
| MsaG041808 | MsaG041814 | 0.816582 | 8.917317e-52 | 1.794483e-49 |
| MsaG041808 | MsaG041826 | 0.810420 | 1.989431e-50 | 3.432523e-48 |
| MsaG041808 | MsaG041835 | 0.812190 | 8.249636e-51 | 1.486391e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG041808 | MtrunA17_Chr7g0269781 | 88.991 | 327 | 31 | 2 | 1 | 322 | 1 | 327 | 0.0 | 583 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG041808 | AT1G01350.1 | 68.790 | 314 | 92 | 4 | 13 | 322 | 25 | 336 | 4.44e-158 | 446 |
| MsaG041808 | AT5G06420.1 | 67.722 | 316 | 93 | 4 | 11 | 322 | 61 | 371 | 8.92e-157 | 444 |
| MsaG041808 | AT5G06420.2 | 67.722 | 316 | 93 | 4 | 11 | 322 | 61 | 371 | 8.92e-157 | 444 |
| MsaG041808 | AT4G01023.1 | 66.667 | 57 | 19 | 0 | 255 | 311 | 78 | 134 | 1.24e-26 | 105 |
| MsaG041808 | AT4G01023.3 | 66.667 | 57 | 19 | 0 | 255 | 311 | 97 | 153 | 3.49e-26 | 104 |
| MsaG041808 | AT4G01023.2 | 66.667 | 57 | 19 | 0 | 255 | 311 | 97 | 153 | 4.49e-26 | 104 |
Find 69 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGAAAGCTAGGTTTCT+TGG | 0.236965 | 8:+12123780 | None:intergenic |
| GTGGGTCACACGGTCCTCTT+CGG | 0.314266 | 8:-12123516 | MsaT041808.1:CDS |
| TTGGTGATTTGCAGGAGATT+TGG | 0.316183 | 8:+12124127 | None:intergenic |
| CTCATATTCTTCTTATTAAG+AGG | 0.321707 | 8:+12123952 | None:intergenic |
| TGTTCAGTTTGGTGATTTGC+AGG | 0.323435 | 8:+12124119 | None:intergenic |
| GATCGAGGTGATTACAAATC+TGG | 0.331028 | 8:-12123382 | MsaT041808.1:CDS |
| TATACATACCTTGTTCAGTT+TGG | 0.352276 | 8:+12124108 | None:intergenic |
| TGGGTCACACGGTCCTCTTC+GGG | 0.377787 | 8:-12123515 | MsaT041808.1:CDS |
| GGATATATGCAAGGATTATA+AGG | 0.382708 | 8:-12123449 | MsaT041808.1:CDS |
| TGCAAGGATTATAAGGAAAC+TGG | 0.390916 | 8:-12123442 | MsaT041808.1:CDS |
| TTATAATCCTTGCATATATC+CGG | 0.408034 | 8:+12123451 | None:intergenic |
| GGTTTCTTGGATTCTTCGTT+CGG | 0.408554 | 8:+12123793 | None:intergenic |
| TAGCAAAGCAACCGCAACTT+TGG | 0.426930 | 8:-12123725 | MsaT041808.1:CDS |
| CTTGCTTAAGAGCTCTTTCA+CGG | 0.431115 | 8:+12123665 | None:intergenic |
| CTGTGAGCATTGTGCATTGA+AGG | 0.436410 | 8:-12123176 | MsaT041808.1:intron |
| GTCTGCAACCAGCCTACGCT+TGG | 0.437064 | 8:-12123032 | MsaT041808.1:CDS |
| TGCTTGCACTTGGTTGAGAC+AGG | 0.443250 | 8:+12123205 | None:intergenic |
| GAAGACTCAAAATGAAAGCT+AGG | 0.455285 | 8:+12123772 | None:intergenic |
| GAAACTGGTTACTGTGGGTA+TGG | 0.457746 | 8:-12123427 | MsaT041808.1:CDS |
| CATTTGTAGAAATCCATTTG+TGG | 0.461066 | 8:-12123230 | MsaT041808.1:CDS |
| GAAAGCAAGGAGGATGAGAT+TGG | 0.475774 | 8:-12123326 | MsaT041808.1:CDS |
| CAATAATTACACAGATCATA+AGG | 0.476447 | 8:-12123581 | MsaT041808.1:CDS |
| GTGGCAGATGGAGAAGGAAT+GGG | 0.495492 | 8:-12123359 | MsaT041808.1:CDS |
| GCAAACAATTGCCAGTGAAA+AGG | 0.499122 | 8:-12123542 | MsaT041808.1:CDS |
| AAACTGGTTACTGTGGGTAT+GGG | 0.503271 | 8:-12123426 | MsaT041808.1:CDS |
| GTTGAGACAGGATCCACAAA+TGG | 0.505798 | 8:+12123217 | None:intergenic |
| CTACAAATGAAACACGCAAA+TGG | 0.511076 | 8:+12123244 | None:intergenic |
| ATCGAGGTGATTACAAATCT+GGG | 0.520829 | 8:-12123381 | MsaT041808.1:CDS |
| AGCAAGATTTGATTATCAGC+CGG | 0.527438 | 8:-12123470 | MsaT041808.1:CDS |
| TTCTTCGTTCGGTTTAGCAT+AGG | 0.529758 | 8:+12123804 | None:intergenic |
| TGCAGGTGAGGATGCAGAAG+AGG | 0.532110 | 8:-12123302 | MsaT041808.1:CDS |
| GGTGGCAGATGGAGAAGGAA+TGG | 0.534084 | 8:-12123360 | MsaT041808.1:CDS |
| CTCTTAATAAGAAGAATATG+AGG | 0.536262 | 8:-12123951 | MsaT041808.1:CDS |
| CTTAAGAGCTCTTTCACGGA+TGG | 0.538525 | 8:+12123669 | None:intergenic |
| AATTACACAGATCATAAGGC+TGG | 0.540860 | 8:-12123577 | MsaT041808.1:CDS |
| GCGAAGCACTATTGAGAATG+AGG | 0.543921 | 8:-12123926 | MsaT041808.1:CDS |
| TTACAAATCTGGGTGGCAGA+TGG | 0.553483 | 8:-12123371 | MsaT041808.1:CDS |
| TATTAGTATGGAAGATTCTG+AGG | 0.556688 | 8:-12124150 | None:intergenic |
| ACAGAAGTAGTGCTTGCACT+TGG | 0.557721 | 8:+12123195 | None:intergenic |
| TCATGAGATTCGAAAGAAGA+TGG | 0.559186 | 8:-12122994 | MsaT041808.1:CDS |
| CAAGCGACAGAGAGTCTCAA+AGG | 0.560300 | 8:-12123646 | MsaT041808.1:CDS |
| AACTGGTTACTGTGGGTATG+GGG | 0.562522 | 8:-12123425 | MsaT041808.1:CDS |
| AGGTGAGGATGCAGAAGAGG+AGG | 0.575195 | 8:-12123299 | MsaT041808.1:CDS |
| ATCTGGGTGGCAGATGGAGA+AGG | 0.575589 | 8:-12123365 | MsaT041808.1:CDS |
| GGGATGAAGCTGAGAAAGCA+AGG | 0.584805 | 8:-12123339 | MsaT041808.1:CDS |
| TATAAGGAAACTGGTTACTG+TGG | 0.586008 | 8:-12123433 | MsaT041808.1:CDS |
| ACAATTGCCAGTGAAAAGGC+TGG | 0.588175 | 8:-12123538 | MsaT041808.1:CDS |
| GATGAGATTGGCTGCAGGTG+AGG | 0.590274 | 8:-12123314 | MsaT041808.1:CDS |
| GGCAGAGCTCTTTGAAGAGG+CGG | 0.591989 | 8:+12123825 | None:intergenic |
| GCAAATCACCAAACTGAACA+AGG | 0.596043 | 8:-12124116 | MsaT041808.1:intron |
| AGAGCTCTTTGAAGAGGCGG+TGG | 0.597520 | 8:+12123828 | None:intergenic |
| ATAGGCAGAGCTCTTTGAAG+AGG | 0.600392 | 8:+12123822 | None:intergenic |
| GAAAAGGCTGGTGGGTCACA+CGG | 0.600903 | 8:-12123526 | MsaT041808.1:CDS |
| AGGAGGATGAGATTGGCTGC+AGG | 0.603143 | 8:-12123319 | MsaT041808.1:CDS |
| ATTGCCAGTGAAAAGGCTGG+TGG | 0.607353 | 8:-12123535 | MsaT041808.1:CDS |
| AGCACTATTGAGAATGAGGA+TGG | 0.610986 | 8:-12123922 | MsaT041808.1:CDS |
| GGTGAGGATGCAGAAGAGGA+GGG | 0.615274 | 8:-12123298 | MsaT041808.1:CDS |
| GATTCGAAAGAAGATGGCTG+AGG | 0.618322 | 8:-12122988 | MsaT041808.1:CDS |
| ATAAGGAAACTGGTTACTGT+GGG | 0.628079 | 8:-12123432 | MsaT041808.1:CDS |
| TTATCAGCCGGATATATGCA+AGG | 0.629970 | 8:-12123458 | MsaT041808.1:CDS |
| TTGCCAGTGAAAAGGCTGGT+GGG | 0.630138 | 8:-12123534 | MsaT041808.1:CDS |
| AGTCTCAGTCTCCAAAGTTG+CGG | 0.639796 | 8:+12123714 | None:intergenic |
| ATGTGAGCTGAAGCCCGAAG+AGG | 0.647511 | 8:+12123502 | None:intergenic |
| TGTAAGTTTATGCATGATCG+AGG | 0.650874 | 8:-12123397 | MsaT041808.1:CDS |
| GAGGATGTAAAATTATACAA+AGG | 0.658301 | 8:-12123607 | MsaT041808.1:CDS |
| TGAGGATGGCTCGAACAATG+AGG | 0.664875 | 8:-12123908 | MsaT041808.1:CDS |
| AGGAAAGAGTAATCGTTCCG+AGG | 0.677145 | 8:-12123626 | MsaT041808.1:CDS |
| ATGAAGCTGAGAAAGCAAGG+AGG | 0.730792 | 8:-12123336 | MsaT041808.1:CDS |
| GAGGTGATTACAAATCTGGG+TGG | 0.758440 | 8:-12123378 | MsaT041808.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 12122980 | 12124165 | 12122980 | ID=MsaG041808 |
| Chr8 | mRNA | 12122980 | 12124165 | 12122980 | ID=MsaT041808.1;Parent=MsaG041808 |
| Chr8 | exon | 12122980 | 12123081 | 12122980 | ID=MsaT041808.1.exon3;Parent=MsaT041808.1 |
| Chr8 | CDS | 12122980 | 12123081 | 12122980 | ID=cds.MsaT041808.1;Parent=MsaT041808.1 |
| Chr8 | exon | 12123177 | 12123994 | 12123177 | ID=MsaT041808.1.exon2;Parent=MsaT041808.1 |
| Chr8 | CDS | 12123177 | 12123994 | 12123177 | ID=cds.MsaT041808.1;Parent=MsaT041808.1 |
| Chr8 | exon | 12124117 | 12124165 | 12124117 | ID=MsaT041808.1.exon1;Parent=MsaT041808.1 |
| Chr8 | CDS | 12124117 | 12124165 | 12124117 | ID=cds.MsaT041808.1;Parent=MsaT041808.1 |
| Gene Sequence |
| Protein sequence |
