AlfalfaGEDB | MsaG041862

M. sativa ssp. caerulea / MsaG041862

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041862	XP_013444538.1	97.452	157	4	0	1	157	181	337	3.91e-103	310

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041862	sp\|O81037\|BH070_ARATH	57.764	161	59	4	1	157	193	348	6.68e-53	175

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041862	tr\|A0A072TNY8\|A0A072TNY8_MEDTR	97.452	157	4	0	1	157	181	337	1.87e-103	310

TFs/TRs:

Gene ID	Type	Classification
MsaG041862	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002888	MsaG041862	0.800277	2.598050e-48	3.534686e-46
MsaG005434	MsaG041862	0.816114	1.133605e-51	2.254075e-49
MsaG006759	MsaG041862	0.843494	2.526412e-58	1.102200e-55
MsaG007372	MsaG041862	0.808504	5.107951e-50	8.413796e-48
MsaG008892	MsaG041862	0.800327	2.538666e-48	3.457792e-46
MsaG009757	MsaG041862	0.801848	1.244562e-48	1.754562e-46
MsaG010331	MsaG041862	0.801237	1.658674e-48	2.306034e-46
MsaG010332	MsaG041862	0.811659	1.075617e-50	1.912857e-48
MsaG010334	MsaG041862	0.804410	3.694718e-49	5.525259e-47
MsaG010573	MsaG041862	0.832867	1.340221e-55	4.214121e-53
MsaG011413	MsaG041862	0.804336	3.826545e-49	5.712797e-47
MsaG012834	MsaG041862	0.809183	3.661505e-50	6.130629e-48
MsaG013804	MsaG041862	0.806329	1.469459e-49	2.298326e-47
MsaG015769	MsaG041862	0.806064	1.670009e-49	2.595618e-47
MsaG016112	MsaG041862	0.817247	6.333073e-52	1.296504e-49
MsaG020193	MsaG041862	0.842667	4.184993e-58	1.777990e-55
MsaG020221	MsaG041862	0.818241	3.787389e-52	7.954455e-50
MsaG021799	MsaG041862	0.854332	2.547529e-61	1.607179e-58
MsaG022388	MsaG041862	0.804088	4.307809e-49	6.394496e-47
MsaG024762	MsaG041862	0.814656	2.382667e-51	4.566055e-49
MsaG028740	MsaG041862	0.808265	5.740926e-50	9.402351e-48
MsaG030244	MsaG041862	0.802398	9.603599e-49	1.371042e-46
MsaG030382	MsaG041862	0.804682	3.243636e-49	4.881353e-47
MsaG030768	MsaG041862	0.826898	3.755371e-54	9.957203e-52
MsaG032531	MsaG041862	0.840419	1.626630e-57	6.434118e-55
MsaG032896	MsaG041862	0.818685	3.007575e-52	6.389728e-50
MsaG033732	MsaG041862	0.800355	2.505385e-48	3.414709e-46
MsaG033878	MsaG041862	0.826378	4.989500e-54	1.303962e-51
MsaG034933	MsaG041862	0.824971	1.071765e-53	2.693925e-51
MsaG037332	MsaG041862	0.803666	5.267089e-49	7.741984e-47
MsaG037736	MsaG041862	0.810518	1.895450e-50	3.278078e-48
MsaG039182	MsaG041862	0.826802	3.958985e-54	1.046872e-51
MsaG040019	MsaG041862	0.802368	9.741629e-49	1.389766e-46
MsaG040039	MsaG041862	0.814221	2.970422e-51	5.630727e-49
MsaG040955	MsaG041862	0.830488	5.136888e-55	1.507440e-52
MsaG041557	MsaG041862	0.842233	5.447267e-58	2.282032e-55
MsaG041550	MsaG041862	0.800078	2.850477e-48	3.860846e-46
MsaG041563	MsaG041862	0.807612	7.891735e-50	1.272330e-47
MsaG041623	MsaG041862	0.823569	2.281146e-53	5.518997e-51
MsaG041862	MsaG044703	0.833828	7.736959e-56	2.502446e-53
MsaG041862	MsaG045314	0.802204	1.052376e-48	1.495666e-46
MsaG041862	MsaG045333	0.817511	5.524824e-52	1.138742e-49
MsaG041862	MsaG045348	0.806341	1.461075e-49	2.285851e-47
MsaG041862	MsaG002656	0.805890	1.816830e-49	2.812180e-47
MsaG041862	MsaG020089	0.805225	2.500921e-49	3.811396e-47
MsaG041862	MsaG018616	0.842380	4.982315e-58	2.097384e-55
MsaG041862	MsaG020276	0.800797	2.038053e-48	2.805662e-46
MsaG041862	MsaG027799	0.800319	2.548103e-48	3.469998e-46
MsaG041862	MsaG033152	0.809729	2.798228e-50	4.747464e-48
MsaG041862	MsaG043204	0.808382	5.421376e-50	8.903743e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041862	MtrunA17_Chr8g0345871	97.452	157	4	0	1	157	181	337	3.60e-107	310
MsaG041862	MtrunA17_Chr3g0132471	51.111	180	68	3	1	162	116	293	4.34e-54	174
MsaG041862	MtrunA17_Chr0c01g0489461	55.429	175	60	4	1	157	195	369	2.02e-52	171
MsaG041862	MtrunA17_Chr8g0366551	50.289	173	71	3	1	161	124	293	1.30e-50	165
MsaG041862	MtrunA17_Chr7g0273081	51.190	168	72	2	1	158	116	283	9.18e-49	160
MsaG041862	MtrunA17_Chr1g0191571	50.581	172	71	3	1	158	136	307	4.65e-48	159
MsaG041862	MtrunA17_Chr5g0426851	45.810	179	75	4	1	162	100	273	1.85e-46	154
MsaG041862	MtrunA17_Chr2g0287761	44.970	169	85	1	1	161	1	169	1.04e-40	135
MsaG041862	MtrunA17_Chr1g0158941	36.022	186	90	3	1	157	87	272	5.23e-32	116
MsaG041862	MtrunA17_Chr1g0185621	30.986	142	91	3	3	139	476	615	4.67e-15	72.0
MsaG041862	MtrunA17_Chr5g0400621	28.188	149	102	2	3	151	158	301	3.34e-12	63.5
MsaG041862	MtrunA17_Chr4g0052841	27.746	173	102	4	3	161	293	456	1.49e-11	62.0
MsaG041862	MtrunA17_Chr1g0211081	27.869	183	99	6	3	157	318	495	3.11e-11	60.8
MsaG041862	MtrunA17_Chr7g0252041	29.012	162	102	5	3	154	287	445	5.07e-11	60.1
MsaG041862	MtrunA17_Chr2g0333621	28.571	161	110	3	1	161	137	292	8.12e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG041862	AT2G46810.2	57.927	164	54	4	1	157	121	276	1.08e-54	175
MsaG041862	AT2G46810.4	57.407	162	60	4	1	158	121	277	2.28e-54	175
MsaG041862	AT2G46810.3	57.764	161	59	4	1	157	181	336	5.35e-54	175
MsaG041862	AT2G46810.1	57.764	161	59	4	1	157	193	348	6.79e-54	175
MsaG041862	AT4G01460.1	52.941	170	70	3	1	161	115	283	5.92e-50	163
MsaG041862	AT4G01460.2	52.941	170	70	3	1	161	115	283	5.92e-50	163
MsaG041862	AT3G24140.2	51.977	177	65	2	1	157	197	373	6.51e-49	163
MsaG041862	AT3G24140.1	51.977	177	65	2	1	157	197	373	7.85e-49	163
MsaG041862	AT1G22490.1	49.711	173	70	3	1	162	115	281	8.99e-48	157
MsaG041862	AT1G72210.2	48.810	168	76	3	1	159	125	291	1.40e-46	154
MsaG041862	AT1G22490.2	50.296	169	67	3	1	158	115	277	1.63e-46	155
MsaG041862	AT1G72210.1	48.235	170	78	3	1	161	125	293	7.88e-46	153
MsaG041862	AT3G61950.6	52.795	161	68	3	1	158	127	282	3.57e-45	150
MsaG041862	AT3G61950.2	53.125	160	67	3	1	157	127	281	6.88e-45	150
MsaG041862	AT3G61950.3	52.795	161	68	3	1	158	178	333	1.39e-44	150
MsaG041862	AT3G61950.1	53.125	160	67	3	1	157	178	332	2.38e-44	150
MsaG041862	AT3G06120.1	43.605	172	82	3	1	157	1	172	2.07e-39	133
MsaG041862	AT5G46690.2	44.149	188	74	4	1	157	88	275	9.81e-33	118
MsaG041862	AT5G46690.1	44.149	188	74	4	1	157	88	275	2.98e-32	118
MsaG041862	AT5G65320.2	41.317	167	89	3	1	158	102	268	1.62e-30	112
MsaG041862	AT5G65320.1	40.828	169	91	3	1	160	102	270	2.25e-30	112
MsaG041862	AT5G53210.2	66.667	63	21	0	1	63	102	164	6.95e-25	99.0
MsaG041862	AT5G53210.1	66.667	63	21	0	1	63	102	164	7.73e-25	99.0
MsaG041862	AT1G12860.2	31.677	161	93	5	3	154	268	420	5.89e-14	68.9
MsaG041862	AT1G12860.1	31.677	161	93	5	3	154	268	420	7.50e-14	68.9
MsaG041862	AT3G26744.6	31.098	164	94	5	3	154	308	464	7.90e-14	68.9
MsaG041862	AT3G26744.1	31.098	164	94	5	3	154	308	464	8.39e-14	68.6
MsaG041862	AT3G26744.4	31.098	164	94	5	3	154	308	464	8.39e-14	68.6
MsaG041862	AT3G26744.2	31.098	164	94	5	3	154	308	464	8.39e-14	68.6
MsaG041862	AT2G16910.1	29.775	178	96	6	3	157	315	486	2.00e-12	64.7
MsaG041862	AT4G29930.2	28.662	157	97	4	3	158	55	197	2.39e-11	60.5
MsaG041862	AT4G29930.5	29.252	147	89	4	3	148	55	187	5.59e-11	59.7
MsaG041862	AT4G29930.6	30.380	158	96	5	3	158	55	200	8.77e-11	58.9

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTCTTATGTAGACATATT+TGG	0.120144	8:+13008425	None:intergenic
CTTCTTATGTAGACATATTT+GGG	0.246365	8:+13008426	None:intergenic
TTGTTGCTTTGGAAAATCTT+AGG	0.306717	8:-13013743	MsaT041862.1:CDS
GATAAAGGTTATTGTTGCTT+TGG	0.325174	8:-13013754	MsaT041862.1:CDS
GTGGACTTGGGGATAGAGGG+TGG	0.349310	8:-13008451	MsaT041862.1:CDS
GGTGACCAAGCATCAATAAT+TGG	0.351169	8:-13014029	MsaT041862.1:CDS
AAGGCACATGGTGGTGGACT+TGG	0.355908	8:-13008464	MsaT041862.1:CDS
ACTCATAAGATGTGATGTTT+AGG	0.372626	8:+13013706	None:intergenic
ATGACCACCTTAGTGTTCTT+AGG	0.380570	8:-13014472	MsaT041862.1:CDS
CACTAAGGTGGTCATTCATT+TGG	0.381725	8:+13014480	None:intergenic
GGACTTGGGGATAGAGGGTG+GGG	0.382784	8:-13008449	MsaT041862.1:CDS
ATATTGCTGTTGAACGTAAT+AGG	0.401299	8:-13014508	MsaT041862.1:CDS
CTCCTCCAATTATTGATGCT+TGG	0.405749	8:+13014024	None:intergenic
AAAGGCTGGTCAATTGATAA+AGG	0.417902	8:-13013769	MsaT041862.1:CDS
AGGCACATGGTGGTGGACTT+GGG	0.427839	8:-13008463	MsaT041862.1:CDS
GGTGGTGGACTTGGGGATAG+AGG	0.433019	8:-13008455	MsaT041862.1:CDS
TCATCATCACCACCTAGTTA+TGG	0.465171	8:-13013909	MsaT041862.1:CDS
TGCCATCTTCATATGTTCAA+AGG	0.473511	8:-13014442	MsaT041862.1:CDS
GAATAATACCTTGAGATTGA+AGG	0.480462	8:+13013668	None:intergenic
GCCATCTTCATATGTTCAAA+GGG	0.484046	8:-13014441	MsaT041862.1:intron
GTGGTGGACTTGGGGATAGA+GGG	0.498492	8:-13008454	MsaT041862.1:CDS
TGGACTTGGGGATAGAGGGT+GGG	0.498650	8:-13008450	MsaT041862.1:CDS
TTGTGAAGAAAATGAGGTAA+AGG	0.510975	8:-13013871	MsaT041862.1:CDS
GATAATTTCATTCCATAACT+AGG	0.511449	8:+13013897	None:intergenic
TTTATAAAGGCACATGGTGG+TGG	0.515501	8:-13008470	MsaT041862.1:intron
ACCCTTTGAACATATGAAGA+TGG	0.543479	8:+13014440	None:intergenic
ATCACCTTGTGAAGAAAATG+AGG	0.546176	8:-13013877	MsaT041862.1:CDS
GGCACATGGTGGTGGACTTG+GGG	0.559908	8:-13008462	MsaT041862.1:CDS
TAAGAGACCTAAGAACACTA+AGG	0.612418	8:+13014465	None:intergenic
ACTCTACTCCTTCAATCTCA+AGG	0.617899	8:-13013676	MsaT041862.1:intron
GACCAAGCATCAATAATTGG+AGG	0.640978	8:-13014026	MsaT041862.1:CDS
TTTATCTTTATAAAGGCACA+TGG	0.641816	8:-13008476	MsaT041862.1:intron
AGGTGGTGATGATGATGATG+AGG	0.648803	8:+13013917	None:intergenic
ATCTTTATAAAGGCACATGG+TGG	0.666941	8:-13008473	MsaT041862.1:intron
AATTTCATTCCATAACTAGG+TGG	0.671528	8:+13013900	None:intergenic
GAGACCTAAGAACACTAAGG+TGG	0.679484	8:+13014468	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	13008430	13014537	13008430	ID=MsaG041862
Chr8	mRNA	13008430	13014537	13008430	ID=MsaT041862.1;Parent=MsaG041862
Chr8	exon	13008430	13008483	13008430	ID=MsaT041862.1.exon3;Parent=MsaT041862.1
Chr8	CDS	13008430	13008483	13008430	ID=cds.MsaT041862.1;Parent=MsaT041862.1
Chr8	exon	13013677	13014051	13013677	ID=MsaT041862.1.exon2;Parent=MsaT041862.1
Chr8	CDS	13013677	13014051	13013677	ID=cds.MsaT041862.1;Parent=MsaT041862.1
Chr8	exon	13014442	13014537	13014442	ID=MsaT041862.1.exon1;Parent=MsaT041862.1
Chr8	CDS	13014442	13014537	13014442	ID=cds.MsaT041862.1;Parent=MsaT041862.1

Gene Sequence

>MsaT041862.1

ATGACACATATTGCTGTTGAACGTAATAGGAGACGCCAAATGAATGACCACCTTAGTGTTCTTAGGTCTCTTATGCCATCTTCATATGTTCAAAGGGGTGACCAAGCATCAATAATTGGAGGAGCAATTGATTTTGTGAAAGAGTTGGAACAATTGCTTGAATCTCTTGAAGCACAAAAAAGGGTAAAAAAGAATGAAGATTTTGGGCCCTCATCATCATCATCACCACCTAGTTATGGAATGAAATTATCACCTTGTGAAGAAAATGAGGTAAAGGCAGAAAACAAATCAGAAGCAGCAGATATAAAAGTAACATTGATTCAAACCCATGTGAACTTGAAAATTGAGTGCAAAAAAAAGGCTGGTCAATTGATAAAGGTTATTGTTGCTTTGGAAAATCTTAGGCTAACTATTCTTCACCTAAACATCACATCTTATGAGTCTTCTGTACTCTACTCCTTCAATCTCAAGGCACATGGTGGTGGACTTGGGGATAGAGGGTGGGGCCCAAATATGTCTACATAA

Protein sequence

>MsaT041862.1

MTHIAVERNRRRQMNDHLSVLRSLMPSSYVQRGDQASIIGGAIDFVKELEQLLESLEAQKRVKKNEDFGPSSSSSPPSYGMKLSPCEENEVKAENKSEAADIKVTLIQTHVNLKIECKKKAGQLIKVIVALENLRLTILHLNITSYESSVLYSFNLKAHGGGLGDRGWGPNMST*