AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041892

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041892 MtrunA17_Chr8g0346161 94.481 453 23 1 12 462 12 464 0.0 890
MsaG041892 MtrunA17_Chr8g0346151 79.861 432 66 8 38 460 35 454 0.0 684
MsaG041892 MtrunA17_Chr5g0411341 50.990 202 88 4 270 461 474 674 4.33e-55 194
MsaG041892 MtrunA17_Chr5g0411341 32.432 185 92 3 25 180 44 224 2.33e-28 118
MsaG041892 MtrunA17_Chr8g0366751 48.020 202 92 4 271 461 444 643 1.43e-51 184
MsaG041892 MtrunA17_Chr8g0366751 31.795 195 100 6 25 190 53 243 2.99e-25 108
MsaG041892 MtrunA17_Chr7g0260841 28.631 482 241 15 19 440 43 481 2.20e-41 154
MsaG041892 MtrunA17_Chr5g0411331 31.720 186 94 4 25 181 27 208 7.85e-29 114
MsaG041892 MtrunA17_Chr7g0275821 37.342 158 90 1 283 440 246 394 1.77e-27 114
MsaG041892 MtrunA17_Chr1g0185621 33.526 173 103 3 281 441 462 634 9.73e-19 89.4
MsaG041892 MtrunA17_Chr1g0185621 28.141 199 98 6 19 181 4 193 1.55e-18 88.6
MsaG041892 MtrunA17_Chr4g0054561 30.500 200 111 7 243 426 102 289 4.27e-18 85.1
MsaG041892 MtrunA17_Chr4g0002781 27.363 201 100 5 252 426 133 313 2.49e-17 83.2
MsaG041892 MtrunA17_Chr2g0333641 30.625 160 102 5 272 426 116 271 1.74e-16 80.1
MsaG041892 MtrunA17_Chr7g0225901 28.481 158 98 3 289 439 88 237 2.63e-16 78.6
MsaG041892 MtrunA17_Chr2g0333621 25.000 160 114 2 272 426 117 275 4.31e-16 79.0
MsaG041892 MtrunA17_Chr4g0002801 30.435 161 95 5 275 426 142 294 4.44e-16 79.3
MsaG041892 MtrunA17_Chr5g0400671 30.899 178 108 5 290 459 155 325 4.99e-16 79.0
MsaG041892 MtrunA17_Chr2g0307131 28.402 169 116 2 258 426 152 315 8.84e-16 78.6
MsaG041892 MtrunA17_Chr5g0400661 28.063 253 148 10 183 418 52 287 1.92e-15 77.4
MsaG041892 MtrunA17_Chr4g0049331 28.161 174 103 4 263 428 114 273 2.48e-15 76.6
MsaG041892 MtrunA17_Chr1g0211081 27.753 227 123 4 241 428 259 483 2.92e-15 78.2
MsaG041892 MtrunA17_Chr4g0033291 28.313 166 103 3 262 426 138 288 3.75e-15 76.3
MsaG041892 MtrunA17_Chr2g0307141 30.496 141 91 2 289 426 183 319 4.06e-15 76.6
MsaG041892 MtrunA17_Chr6g0468491 33.333 144 87 4 260 401 112 248 4.08e-15 76.3
MsaG041892 MtrunA17_Chr2g0287761 26.136 176 116 4 288 451 1 174 7.51e-15 73.2
MsaG041892 MtrunA17_Chr4g0033391 26.857 175 107 5 258 426 121 280 8.07e-15 75.5
MsaG041892 MtrunA17_Chr4g0054551 28.643 199 115 7 243 428 102 286 1.13e-14 75.1
MsaG041892 MtrunA17_Chr2g0280161 31.034 145 84 3 289 425 177 313 3.72e-14 73.6
MsaG041892 MtrunA17_Chr8g0386921 38.835 103 53 2 288 380 405 507 6.37e-14 73.9
MsaG041892 MtrunA17_Chr7g0252041 60.000 55 22 0 287 341 284 338 7.65e-14 73.6
MsaG041892 MtrunA17_Chr3g0131691 58.182 55 23 0 287 341 296 350 7.77e-14 73.6
MsaG041892 MtrunA17_Chr6g0468461 37.838 111 61 2 260 370 148 250 1.02e-13 72.4
MsaG041892 MtrunA17_Chr4g0033281 25.728 206 131 6 263 462 137 326 1.40e-13 71.6
MsaG041892 MtrunA17_Chr4g0054571 31.373 153 93 4 274 418 134 282 1.97e-13 71.2
MsaG041892 MtrunA17_Chr2g0298751 53.226 62 22 1 290 344 53 114 3.79e-13 69.3
MsaG041892 MtrunA17_Chr4g0054581 49.254 67 30 1 289 355 198 260 6.31e-13 70.1
MsaG041892 MtrunA17_Chr8g0336681 25.316 158 112 2 275 426 142 299 1.65e-12 68.6
MsaG041892 MtrunA17_Chr4g0052841 29.012 162 84 5 290 431 293 443 5.19e-12 67.8
MsaG041892 MtrunA17_Chr4g0028071 30.000 160 71 7 293 431 153 292 2.92e-11 64.7
MsaG041892 MtrunA17_Chr2g0320291 49.020 51 26 0 293 343 137 187 8.33e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041892 AT4G00870.1 37.527 461 200 14 20 460 29 421 9.57e-73 236
MsaG041892 AT4G17880.1 28.864 537 270 13 26 462 63 587 2.35e-68 229
MsaG041892 AT5G46830.1 33.333 525 236 18 18 460 18 510 6.26e-65 218
MsaG041892 AT1G32640.1 51.980 202 89 4 263 461 427 623 1.63e-56 198
MsaG041892 AT1G32640.1 31.053 190 94 3 25 181 67 252 9.05e-30 122
MsaG041892 AT5G46760.1 44.770 239 120 5 229 462 359 590 6.65e-54 190
MsaG041892 AT5G46760.1 32.065 184 89 5 25 176 51 230 1.08e-26 113
MsaG041892 AT4G16430.1 27.215 474 262 13 21 462 45 467 7.98e-41 152
MsaG041892 AT2G46510.1 41.714 175 82 3 269 443 376 530 2.20e-34 135
MsaG041892 AT1G01260.1 38.889 180 85 3 273 452 417 571 3.28e-30 124
MsaG041892 AT1G01260.1 28.061 196 100 5 26 184 48 239 3.44e-13 72.0
MsaG041892 AT1G01260.2 38.889 180 85 3 273 452 417 571 3.28e-30 124
MsaG041892 AT1G01260.2 28.061 196 100 5 26 184 48 239 3.44e-13 72.0
MsaG041892 AT1G01260.3 38.889 180 85 3 273 452 417 571 3.28e-30 124
MsaG041892 AT1G01260.3 28.061 196 100 5 26 184 48 239 3.44e-13 72.0
MsaG041892 AT4G00480.1 22.201 518 296 15 31 461 26 523 1.49e-19 91.7
MsaG041892 AT4G00480.2 22.549 510 294 13 31 456 26 518 2.74e-19 91.3
MsaG041892 AT4G00480.3 22.691 498 278 15 31 441 26 503 3.40e-19 90.5
MsaG041892 AT1G63650.3 26.486 185 119 3 289 456 405 589 2.75e-16 82.0
MsaG041892 AT1G63650.1 26.486 185 119 3 289 456 405 589 2.75e-16 82.0
MsaG041892 AT1G63650.2 26.486 185 119 3 289 456 405 589 2.75e-16 82.0
MsaG041892 AT4G37850.2 31.325 166 104 5 266 426 130 290 3.88e-16 79.7
MsaG041892 AT4G37850.1 31.325 166 104 5 266 426 130 290 3.88e-16 79.7
MsaG041892 AT4G09820.1 33.588 131 64 2 288 417 362 470 5.66e-15 77.4
MsaG041892 AT3G26744.6 60.000 55 22 0 287 341 305 359 1.72e-14 75.9
MsaG041892 AT3G26744.1 60.000 55 22 0 287 341 305 359 1.77e-14 75.9
MsaG041892 AT3G26744.4 60.000 55 22 0 287 341 305 359 1.77e-14 75.9
MsaG041892 AT3G26744.2 60.000 55 22 0 287 341 305 359 1.77e-14 75.9
MsaG041892 AT1G12860.2 57.895 57 24 0 287 343 265 321 1.80e-14 75.5
MsaG041892 AT1G12860.1 57.895 57 24 0 287 343 265 321 2.14e-14 75.5
MsaG041892 AT5G57150.1 26.908 249 138 10 233 461 7 231 7.94e-14 71.6
MsaG041892 AT5G57150.2 27.193 228 128 8 233 441 7 215 1.41e-13 70.5
MsaG041892 AT5G57150.4 27.468 233 126 10 233 443 7 218 1.65e-13 70.9
MsaG041892 AT3G06120.1 27.869 183 111 4 288 449 1 183 1.86e-13 69.7
MsaG041892 AT5G57150.3 26.754 228 129 8 233 441 7 215 2.13e-13 69.7
MsaG041892 AT4G29930.5 54.098 61 21 1 290 343 55 115 2.22e-13 70.1
MsaG041892 AT4G29930.2 54.098 61 21 1 290 343 55 115 2.59e-13 69.7
MsaG041892 AT4G29930.6 54.098 61 21 1 290 343 55 115 2.74e-13 69.7
MsaG041892 AT5G57150.5 36.719 128 59 5 233 359 7 113 2.78e-13 69.7
MsaG041892 AT4G29930.4 55.000 60 20 1 291 343 56 115 4.28e-13 68.2
MsaG041892 AT4G29930.3 54.098 61 21 1 290 343 55 115 4.60e-13 69.7
MsaG041892 AT4G29930.7 54.098 61 21 1 290 343 55 115 4.60e-13 69.7
MsaG041892 AT4G29930.1 54.098 61 21 1 290 343 55 115 5.12e-13 69.3
MsaG041892 AT1G10610.2 32.847 137 85 4 290 421 235 369 1.80e-12 69.3
MsaG041892 AT1G10610.1 32.847 137 85 4 290 421 265 399 2.14e-12 69.3
MsaG041892 AT1G10610.3 32.847 137 85 4 290 421 168 302 2.18e-12 68.6
MsaG041892 AT5G41315.2 32.258 93 49 1 289 381 449 527 2.89e-12 69.3
MsaG041892 AT5G41315.3 32.258 93 49 1 289 381 446 524 2.95e-12 69.3
MsaG041892 AT5G41315.1 32.258 93 49 1 289 381 441 519 3.07e-12 69.3
MsaG041892 AT2G22760.2 28.415 183 106 7 276 439 100 276 7.46e-12 66.2
MsaG041892 AT2G16910.1 54.000 50 23 0 293 342 318 367 1.11e-11 67.4
MsaG041892 AT3G24140.1 23.721 215 137 4 269 456 175 389 1.69e-11 66.2
MsaG041892 AT2G22760.1 29.697 165 96 6 276 426 100 258 1.95e-11 65.1
MsaG041892 AT3G24140.2 23.721 215 137 4 269 456 175 389 2.29e-11 65.9

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCATTTGTTGCAAATTT+TGG 0.152440 8:+13441315 None:intergenic
TCCAAACCCAATCCGAAAAT+TGG 0.161848 8:-13441901 MsaT041892.1:CDS
ATTGAAGGATTTGGAGTTTC+AGG 0.165432 8:-13440763 MsaT041892.1:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8:-13440852 MsaT041892.1:CDS
GTTTGAAATTGTTGTTTCTT+TGG 0.232211 8:+13441796 None:intergenic
AAGGTAAACCTTTCTTATCT+TGG 0.238310 8:-13441841 MsaT041892.1:CDS
ATGACAATATCCAACAAAAT+TGG 0.270722 8:-13441511 MsaT041892.1:CDS
TTATCCATCTTTGAAACATT+TGG 0.290544 8:+13441065 None:intergenic
AGGTAAACCTTTCTTATCTT+GGG 0.292720 8:-13441840 MsaT041892.1:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8:+13441096 None:intergenic
AAGATTGAAGATTTAGAATC+AGG 0.311502 8:-13440993 MsaT041892.1:CDS
AATGAGTGTATTGAAGGATT+TGG 0.327266 8:-13440772 MsaT041892.1:CDS
TACCGATGTCGGCGAATGTT+TGG 0.330440 8:+13441387 None:intergenic
ATTCTTGTTGGTGTTGCTAA+TGG 0.335278 8:+13441236 None:intergenic
TCCATGCACGTGTGCTTCAT+TGG 0.351119 8:+13441589 None:intergenic
AGGGTTAGGGTTTGTGGTGA+AGG 0.352816 8:+13441947 None:intergenic
TGTTGAAGGGTTAGGGTTTG+TGG 0.360355 8:+13441941 None:intergenic
AACTAACGGTGTAATCGAAA+TGG 0.368563 8:-13441540 MsaT041892.1:CDS
ATCAGTGTCTTCTCCGGAAC+CGG 0.379047 8:+13441358 None:intergenic
AGAGGAAGAAAACCGTTATC+AGG 0.381422 8:-13441176 MsaT041892.1:CDS
TCAGCCATTCTCATGAAATT+AGG 0.386390 8:-13440642 MsaT041892.1:CDS
TTGTTGTTTCTTTGGTTCCT+TGG 0.388661 8:+13441804 None:intergenic
TAGATTCTGATGTGGATGTT+TGG 0.396023 8:+13441459 None:intergenic
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8:-13440724 MsaT041892.1:CDS
TAGATTTGGCTTGGTGTACT+AGG 0.400415 8:+13441486 None:intergenic
CAAACAACAAAAGATGAAAA+AGG 0.404302 8:-13441860 MsaT041892.1:CDS
ATCGGTATCGTCTCCGGTTC+CGG 0.406820 8:-13441371 MsaT041892.1:CDS
GGATGTTTGGAACATAGATT+TGG 0.408652 8:+13441472 None:intergenic
TCTGCTTCCACATGGTTTAA+CGG 0.415651 8:+13441143 None:intergenic
AAGATTAATGAGTGTATTGA+AGG 0.416800 8:-13440778 MsaT041892.1:CDS
GGGTTAGGGTTTGTGGTGAA+GGG 0.421882 8:+13441948 None:intergenic
TTGCTTTAGATTCTGTACTT+TGG 0.425852 8:-13441655 MsaT041892.1:CDS
AGATTTGTCAAGTGGGTTTG+TGG 0.429194 8:+13441415 None:intergenic
GGTTCCAAATGTTTCAAAGA+TGG 0.435449 8:-13441069 MsaT041892.1:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8:-13440952 MsaT041892.1:CDS
CCTTTCTTATCTTGGGGTGA+AGG 0.446476 8:-13441833 MsaT041892.1:CDS
TCTTCCGCGCTCATCTCGTT+AGG 0.463829 8:+13440678 None:intergenic
CCAAACCCAATCCGAAAATT+GGG 0.468035 8:-13441900 MsaT041892.1:CDS
TGTCTCATCAGTGTCTTCTC+CGG 0.468594 8:+13441352 None:intergenic
GACAGCAACACGTGTACAAA+TGG 0.479858 8:-13441764 MsaT041892.1:CDS
CTGACCTAATTTCATGAGAA+TGG 0.482002 8:+13440638 None:intergenic
GCTTCTTCAATTTCATTACC+TGG 0.483921 8:-13441686 MsaT041892.1:CDS
ACTAACGGTGTAATCGAAAT+GGG 0.485666 8:-13441539 MsaT041892.1:CDS
CCTTCACCCCAAGATAAGAA+AGG 0.497048 8:+13441833 None:intergenic
TCGATTACACCGTTAGTTGT+CGG 0.498052 8:+13441545 None:intergenic
ATTTGTATTCCGACAACTAA+CGG 0.507792 8:-13441554 MsaT041892.1:CDS
GGTGTTTGAGTTCCTGATAA+CGG 0.508977 8:+13441164 None:intergenic
GCCGACATCGGTATCGTCTC+CGG 0.512586 8:-13441377 MsaT041892.1:CDS
TTTAGAATCAGGGCAACAAA+AGG 0.514365 8:-13440982 MsaT041892.1:CDS
TTTGTCTCTCTGCTTCCACA+TGG 0.518910 8:+13441135 None:intergenic
TCCAATGAAGCACACGTGCA+TGG 0.524272 8:-13441590 MsaT041892.1:CDS
TGGAACATAGATTTGTCAAG+TGG 0.527748 8:+13441407 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8:-13441090 MsaT041892.1:CDS
ACACTCATTAATCTTGCACT+AGG 0.528247 8:+13440786 None:intergenic
AATTGAAGAAGCATTACCTA+TGG 0.532558 8:+13441697 None:intergenic
TTTGGAACATAGATTTGGCT+TGG 0.537383 8:+13441477 None:intergenic
GGTAAACCTTTCTTATCTTG+GGG 0.537701 8:-13441839 MsaT041892.1:CDS
TTCCAAACATTCGCCGACAT+CGG 0.537863 8:-13441389 MsaT041892.1:CDS
ATGTGGAAGCAGAGAGACAA+AGG 0.547229 8:-13441133 MsaT041892.1:CDS
ATCATAGAGGAAGATGAAGA+AGG 0.553296 8:+13441995 None:intergenic
TCAAAAGCATTAACATTACA+AGG 0.560113 8:+13440873 None:intergenic
AGATTGAAGATTTAGAATCA+GGG 0.563283 8:-13440992 MsaT041892.1:CDS
CTTGTTGGTGTTGCTAATGG+TGG 0.567083 8:+13441239 None:intergenic
GATAATAGGAAATGATGCTA+TGG 0.577854 8:-13440838 MsaT041892.1:CDS
TGCAACGGCAACTTCAACTG+TGG 0.578390 8:-13440910 MsaT041892.1:CDS
TGGGGTGAAGGCTATTTCCA+AGG 0.580662 8:-13441821 MsaT041892.1:CDS
GGAACATAGATTTGTCAAGT+GGG 0.591339 8:+13441408 None:intergenic
GTACAAATGGTGATGCTGAA+TGG 0.602234 8:-13441751 MsaT041892.1:CDS
TCTAAAGCAAAAGCTTTACC+AGG 0.602957 8:+13441668 None:intergenic
AAAAGAGAACCTAAGAAGAG+AGG 0.611347 8:-13441194 MsaT041892.1:CDS
TTCTGAGTTAGATTCTGATG+TGG 0.614919 8:+13441451 None:intergenic
TGTCGATAATAACAGTGCAA+CGG 0.631393 8:-13440925 MsaT041892.1:CDS
CACTCATTAATCTTGCACTA+GGG 0.639299 8:+13440787 None:intergenic
ATGAGGAAATGATATCATAG+AGG 0.640466 8:+13441982 None:intergenic
ACCGGAGACGATACCGATGT+CGG 0.645053 8:+13441376 None:intergenic
TCAAACACCGTTAAACCATG+TGG 0.685029 8:-13441150 MsaT041892.1:CDS
AGTTCCTAACGAGATGAGCG+CGG 0.706023 8:-13440682 MsaT041892.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 13440635 13442023 13440635 ID=MsaG041892
Chr8 mRNA 13440635 13442023 13440635 ID=MsaT041892.1;Parent=MsaG041892
Chr8 exon 13440635 13442023 13440635 ID=MsaT041892.1.exon1;Parent=MsaT041892.1
Chr8 CDS 13440635 13442023 13440635 ID=cds.MsaT041892.1;Parent=MsaT041892.1
Gene Sequence

>MsaT041892.1

ATGTCACCTTCTTCATCTTCCTCTATGATATCATTTCCTCATGAAAATGAAAACCCTTCACCACAAACCCTAACCCTTCAACAAAAACTTCAATTATTAGTCCAAACCCAATCCGAAAATTGGGTCTATGCCATTTTGTGGCAAACAACAAAAGATGAAAAAGGTAAACCTTTCTTATCTTGGGGTGAAGGCTATTTCCAAGGAACCAAAGAAACAACAATTTCAAACAAACAACACGACAGCAACACGTGTACAAATGGTGATGCTGAATGGTTCTATGTGATGTCGTTGAGTCGAAATTTTTCCATAGGTAATGCTTCTTCAATTTCATTACCTGGTAAAGCTTTTGCTTTAGATTCTGTACTTTGGTTGAATAGTAAACATGAACTTCAAATCTATAACTGTGAGAGATCCAATGAAGCACACGTGCATGGAATTGAAACATTGATTTGTATTCCGACAACTAACGGTGTAATCGAAATGGGTTCTTATGACAATATCCAACAAAATTGGAACCTAGTACACCAAGCCAAATCTATGTTCCAAACATCCACATCAGAATCTAACTCAGAAATAGATCTTATCTCCACAAACCCACTTGACAAATCTATGTTCCAAACATTCGCCGACATCGGTATCGTCTCCGGTTCCGGAGAAGACACTGATGAGACACAAAAAACAATGAACCAAAATTTGCAACAAATGAATAACATTGTTTCATCATGTTCTTTTGATTCTGAAAATTCTGATTCAGAATATTGTCCACCATTAGCAACACCAACAAGAATTGAAAAAGATTCTTTTGAGAAAAGAGAACCTAAGAAGAGAGGAAGAAAACCGTTATCAGGAACTCAAACACCGTTAAACCATGTGGAAGCAGAGAGACAAAGGAGAGAGAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAATGTTTCAAAGATGGATAAAGCTTCTCTTTTATCAGATGCAGTGGATTACATCAACGAGTTAAAAGCAAAGATTGAAGATTTAGAATCAGGGCAACAAAAGGAATCGAAAAAAGTGAAGATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTGAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTATTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCGCGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG
Protein sequence

>MsaT041892.1

MSPSSSSSMISFPHENENPSPQTLTLQQKLQLLVQTQSENWVYAILWQTTKDEKGKPFLSWGEGYFQGTKETTISNKQHDSNTCTNGDAEWFYVMSLSRNFSIGNASSISLPGKAFALDSVLWLNSKHELQIYNCERSNEAHVHGIETLICIPTTNGVIEMGSYDNIQQNWNLVHQAKSMFQTSTSESNSEIDLISTNPLDKSMFQTFADIGIVSGSGEDTDETQKTMNQNLQQMNNIVSSCSFDSENSDSEYCPPLATPTRIEKDSFEKREPKKRGRKPLSGTQTPLNHVEAERQRREKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLESGQQKESKKVKMEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSAEESLIRSAILMKLGQ*