AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG041982

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041982 MtrunA17_Chr8g0347251 80.460 348 16 3 1 296 1 348 0.0 554
MsaG041982 MtrunA17_Chr7g0275531 45.928 307 114 12 12 293 45 324 1.08e-69 219
MsaG041982 MtrunA17_Chr3g0126391 39.526 253 138 9 46 290 89 334 2.80e-46 159
MsaG041982 MtrunA17_Chr8g0387981 35.144 313 160 10 12 290 46 349 5.56e-45 156
MsaG041982 MtrunA17_Chr7g0244971 50.588 85 34 1 63 147 106 182 2.82e-22 94.0
MsaG041982 MtrunA17_Chr7g0244981 49.412 85 35 1 63 147 105 181 7.89e-21 90.1
MsaG041982 MtrunA17_Chr3g0128671 55.224 67 30 0 81 147 120 186 4.10e-20 88.6
MsaG041982 MtrunA17_Chr7g0270051 40.972 144 66 6 43 181 3 132 4.50e-19 84.7
MsaG041982 MtrunA17_Chr8g0350621 44.706 85 39 2 63 147 94 170 6.93e-18 81.6
MsaG041982 MtrunA17_Chr5g0428351 47.222 72 38 0 81 152 176 247 9.41e-18 82.0
MsaG041982 MtrunA17_Chr8g0334541 44.444 90 43 1 62 151 84 166 1.48e-17 80.9
MsaG041982 MtrunA17_Chr2g0315361 43.158 95 45 2 57 150 124 210 1.60e-17 81.3
MsaG041982 MtrunA17_Chr2g0315381 34.783 138 78 3 62 195 125 254 2.95e-17 80.5
MsaG041982 MtrunA17_Chr7g0224801 56.667 60 23 1 87 146 92 148 1.09e-16 76.3
MsaG041982 MtrunA17_Chr4g0071521 41.935 93 51 2 58 150 216 305 5.93e-16 76.6
MsaG041982 MtrunA17_Chr2g0315371 37.500 88 47 1 63 150 124 203 7.12e-16 76.3
MsaG041982 MtrunA17_Chr8g0387501 43.836 73 39 1 81 153 122 192 8.52e-16 75.9
MsaG041982 MtrunA17_Chr1g0147881 42.697 89 48 2 60 148 235 320 1.24e-15 76.3
MsaG041982 MtrunA17_Chr2g0293481 32.857 140 75 4 57 187 239 368 2.81e-15 75.9
MsaG041982 MtrunA17_Chr7g0270191 29.508 183 98 6 63 242 119 273 4.15e-15 74.3
MsaG041982 MtrunA17_Chr8g0379461 30.348 201 109 10 63 247 45 230 1.24e-14 72.0
MsaG041982 MtrunA17_Chr4g0042831 51.724 58 25 1 88 145 145 199 1.68e-14 73.2
MsaG041982 MtrunA17_Chr4g0042831 45.763 59 29 1 87 145 277 332 2.52e-11 63.5
MsaG041982 MtrunA17_Chr5g0401821 44.186 86 45 2 60 145 172 254 1.70e-14 72.0
MsaG041982 MtrunA17_Chr8g0386241 47.458 59 28 1 87 145 102 157 3.43e-14 69.7
MsaG041982 MtrunA17_Chr7g0249311 35.537 121 60 4 30 145 125 232 4.09e-14 72.4
MsaG041982 MtrunA17_Chr2g0284321 31.624 117 73 2 62 178 9 118 4.36e-14 70.1
MsaG041982 MtrunA17_Chr7g0248691 37.755 98 59 1 48 145 114 209 5.71e-14 71.2
MsaG041982 MtrunA17_Chr8g0348311 38.000 100 60 1 47 146 114 211 6.96e-14 70.9
MsaG041982 MtrunA17_Chr3g0079691 35.211 142 78 6 14 145 196 333 1.07e-13 71.2
MsaG041982 MtrunA17_Chr7g0218081 40.426 94 44 5 87 178 227 310 1.08e-13 71.2
MsaG041982 MtrunA17_Chr7g0218081 44.068 59 30 1 87 145 409 464 3.96e-11 63.5
MsaG041982 MtrunA17_Chr5g0431811 47.619 63 33 0 88 150 672 734 1.31e-13 71.2
MsaG041982 MtrunA17_Chr1g0202461 40.476 84 43 2 62 145 240 316 1.53e-13 70.5
MsaG041982 MtrunA17_Chr5g0443131 49.153 59 27 1 87 145 156 211 1.58e-13 69.7
MsaG041982 MtrunA17_Chr1g0194481 39.130 92 48 2 55 146 130 213 2.11e-13 69.7
MsaG041982 MtrunA17_Chr4g0053281 43.678 87 45 3 60 145 238 321 2.29e-13 69.7
MsaG041982 MtrunA17_Chr2g0294231 48.333 60 28 1 87 146 202 258 3.40e-13 69.3
MsaG041982 MtrunA17_Chr5g0418781 50.847 59 26 1 87 145 123 178 3.53e-13 68.6
MsaG041982 MtrunA17_Chr4g0051881 49.153 59 27 1 87 145 177 232 4.96e-13 68.6
MsaG041982 MtrunA17_Chr5g0445311 48.571 70 30 3 78 145 188 253 7.60e-13 68.6
MsaG041982 MtrunA17_Chr7g0238921 46.053 76 34 2 70 145 110 178 9.10e-13 67.8
MsaG041982 MtrunA17_Chr8g0383351 42.466 73 39 1 87 159 159 228 1.25e-12 66.2
MsaG041982 MtrunA17_Chr3g0101131 49.231 65 29 2 81 145 236 296 1.27e-12 68.2
MsaG041982 MtrunA17_Chr3g0101131 38.462 91 46 3 62 145 382 469 4.60e-11 63.2
MsaG041982 MtrunA17_Chr7g0270831 38.095 105 57 3 51 155 180 276 1.66e-12 67.4
MsaG041982 MtrunA17_Chr2g0288871 35.036 137 72 5 29 155 236 365 2.03e-12 67.4
MsaG041982 MtrunA17_Chr5g0418191 42.045 88 42 3 87 174 150 228 2.03e-12 67.0
MsaG041982 MtrunA17_Chr5g0418191 44.068 59 30 1 87 145 319 374 2.18e-11 63.9
MsaG041982 MtrunA17_Chr3g0129361 42.466 73 39 1 87 159 143 212 2.32e-12 65.1
MsaG041982 MtrunA17_Chr2g0322391 52.542 59 26 1 87 145 348 404 3.47e-12 66.6
MsaG041982 MtrunA17_Chr2g0319961 36.082 97 52 3 52 145 46 135 3.60e-12 65.1
MsaG041982 MtrunA17_Chr7g0263531 50.877 57 24 2 89 145 190 242 3.70e-12 66.2
MsaG041982 MtrunA17_Chr4g0068541 45.763 59 29 1 87 145 207 262 5.15e-12 65.9
MsaG041982 MtrunA17_Chr7g0228941 44.595 74 38 2 72 145 37 107 5.46e-12 62.0
MsaG041982 MtrunA17_Chr4g0075201 51.667 60 27 1 87 146 266 323 6.84e-12 65.9
MsaG041982 MtrunA17_Chr5g0447701 46.154 65 32 1 81 145 245 306 9.75e-12 65.1
MsaG041982 MtrunA17_Chr8g0335811 45.588 68 31 2 87 151 153 217 1.03e-11 63.5
MsaG041982 MtrunA17_Chr4g0004911 36.364 110 51 4 59 161 152 249 1.04e-11 65.1
MsaG041982 MtrunA17_Chr1g0184691 47.368 57 28 1 89 145 294 348 1.40e-11 64.3
MsaG041982 MtrunA17_Chr7g0243891 47.222 72 32 3 88 157 216 283 1.54e-11 64.7
MsaG041982 MtrunA17_Chr3g0136961 47.458 59 29 1 87 145 137 193 1.56e-11 63.9
MsaG041982 MtrunA17_Chr3g0099641 43.836 73 34 2 87 159 250 315 1.64e-11 64.3
MsaG041982 MtrunA17_Chr3g0139021 48.387 62 30 1 89 150 315 374 1.65e-11 64.3
MsaG041982 MtrunA17_Chr1g0150281 50.847 59 27 1 87 145 139 195 1.92e-11 63.5
MsaG041982 MtrunA17_Chr3g0106491 45.000 60 30 1 87 146 364 420 2.08e-11 64.3
MsaG041982 MtrunA17_Chr3g0106491 34.146 123 65 4 88 210 198 304 7.75e-11 62.4
MsaG041982 MtrunA17_Chr7g0249301 35.000 100 57 3 79 174 53 148 2.78e-11 63.5
MsaG041982 MtrunA17_Chr5g0432161 48.276 58 26 2 88 145 244 297 5.41e-11 63.2
MsaG041982 MtrunA17_Chr2g0333851 35.955 89 52 2 76 161 154 240 6.65e-11 62.0
MsaG041982 MtrunA17_Chr1g0150801 36.709 79 44 2 71 146 82 157 7.50e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG041982 AT5G24110.1 37.297 185 100 4 45 226 72 243 3.90e-39 139
MsaG041982 AT4G23810.1 44.516 155 69 4 10 155 79 225 2.18e-38 138
MsaG041982 AT4G11070.2 51.493 134 58 3 45 174 64 194 2.94e-37 134
MsaG041982 AT4G11070.1 51.493 134 58 3 45 174 96 226 5.29e-37 134
MsaG041982 AT2G46400.1 42.135 178 88 4 59 224 74 248 9.81e-37 133
MsaG041982 AT2G40750.1 49.020 102 46 2 63 159 122 222 9.96e-24 99.4
MsaG041982 AT3G56400.1 42.500 120 66 1 39 158 74 190 1.20e-22 95.5
MsaG041982 AT2G40740.1 53.125 64 30 0 88 151 173 236 7.83e-20 87.8
MsaG041982 AT2G40740.3 53.125 64 30 0 88 151 182 245 9.61e-20 87.8
MsaG041982 AT5G45260.1 37.762 143 73 3 28 170 1159 1285 6.37e-19 87.4
MsaG041982 AT2G23320.1 35.897 117 64 3 29 145 190 295 2.15e-15 75.5
MsaG041982 AT4G24240.1 38.017 121 62 5 25 145 229 336 3.43e-15 75.5
MsaG041982 AT2G30590.1 40.404 99 52 3 60 158 286 377 6.70e-15 74.7
MsaG041982 AT2G24570.1 41.121 107 57 3 60 163 216 319 1.22e-14 73.6
MsaG041982 AT5G52830.1 43.529 85 41 2 62 146 144 221 1.29e-14 73.6
MsaG041982 AT4G31550.1 40.594 101 57 2 50 150 209 306 1.32e-14 73.6
MsaG041982 AT4G23550.1 33.051 118 72 2 54 171 106 216 1.70e-14 72.8
MsaG041982 AT4G31550.2 44.318 88 46 2 63 150 221 305 3.51e-14 72.0
MsaG041982 AT2G03340.1 46.667 75 31 3 83 157 245 310 3.94e-14 72.8
MsaG041982 AT5G28650.1 44.186 86 45 2 60 145 235 317 4.92e-14 71.6
MsaG041982 AT4G01250.1 44.048 84 39 2 62 145 108 183 4.93e-14 71.6
MsaG041982 AT3G58710.2 40.000 115 56 5 49 158 27 133 7.67e-14 70.5
MsaG041982 AT1G69310.4 36.893 103 56 3 48 150 112 205 7.77e-14 70.9
MsaG041982 AT1G69310.2 36.893 103 56 3 48 150 112 205 7.77e-14 70.9
MsaG041982 AT1G69310.1 36.893 103 56 3 48 150 112 205 7.77e-14 70.9
MsaG041982 AT1G66550.2 43.590 78 42 1 89 164 104 181 9.13e-14 70.1
MsaG041982 AT2G34830.2 33.333 138 81 3 17 145 144 279 1.12e-13 71.2
MsaG041982 AT2G34830.1 33.333 138 81 3 17 145 135 270 1.13e-13 71.2
MsaG041982 AT1G29280.1 35.897 117 58 4 32 145 27 129 1.13e-13 70.1
MsaG041982 AT5G49520.1 48.333 60 28 1 87 146 220 276 1.13e-13 71.2
MsaG041982 AT1G66550.1 43.590 78 42 1 89 164 109 186 1.19e-13 69.7
MsaG041982 AT3G58710.1 38.261 115 59 4 49 158 27 134 1.22e-13 70.1
MsaG041982 AT1G66600.1 39.726 73 44 0 75 147 90 162 1.45e-13 69.3
MsaG041982 AT1G30650.1 40.449 89 48 2 57 145 189 272 1.61e-13 70.9
MsaG041982 AT2G37260.3 40.244 82 46 1 64 145 245 323 2.04e-13 70.1
MsaG041982 AT2G37260.3 40.659 91 46 4 68 158 69 151 5.22e-12 65.9
MsaG041982 AT2G37260.2 40.244 82 46 1 64 145 243 321 2.06e-13 70.1
MsaG041982 AT2G37260.2 40.659 91 46 4 68 158 67 149 5.27e-12 65.9
MsaG041982 AT2G37260.1 40.244 82 46 1 64 145 325 403 2.44e-13 70.5
MsaG041982 AT2G37260.1 40.659 91 46 4 68 158 149 231 4.33e-12 66.6
MsaG041982 AT4G18170.1 42.045 88 47 2 59 145 142 226 3.11e-13 69.3
MsaG041982 AT1G29860.1 41.379 87 42 3 74 159 121 199 3.42e-13 68.9
MsaG041982 AT3G04670.1 43.023 86 46 2 60 145 235 317 3.89e-13 69.3
MsaG041982 AT5G13080.1 50.000 60 27 1 87 146 66 122 4.02e-13 66.2
MsaG041982 AT1G13960.1 46.575 73 30 3 87 159 228 291 4.15e-13 69.7
MsaG041982 AT1G13960.1 33.333 99 60 2 47 145 371 463 2.17e-11 64.7
MsaG041982 AT4G01720.1 39.823 113 58 4 45 155 195 299 4.32e-13 69.7
MsaG041982 AT2G04880.2 47.458 59 28 1 87 145 282 337 4.77e-13 69.7
MsaG041982 AT1G13960.2 46.575 73 30 3 87 159 75 138 6.25e-13 68.9
MsaG041982 AT1G13960.2 44.068 59 30 1 87 145 255 310 4.11e-11 63.5
MsaG041982 AT3G01970.1 50.000 60 27 1 87 146 64 120 6.40e-13 65.5
MsaG041982 AT2G04880.1 47.458 59 28 1 87 145 306 361 6.65e-13 69.3
MsaG041982 AT1G66560.1 38.158 76 47 0 72 147 87 162 7.83e-13 67.4
MsaG041982 AT2G47260.1 40.476 84 47 1 87 170 173 253 9.20e-13 68.2
MsaG041982 AT4G26640.7 38.889 90 39 3 87 176 340 413 1.78e-12 67.8
MsaG041982 AT4G26640.7 49.153 59 26 2 87 145 170 224 1.34e-11 65.1
MsaG041982 AT4G26640.2 38.889 90 39 3 87 176 380 453 1.93e-12 67.8
MsaG041982 AT4G26640.2 49.153 59 26 2 87 145 210 264 1.46e-11 65.1
MsaG041982 AT2G46130.1 47.458 59 28 1 87 145 29 84 2.10e-12 63.2
MsaG041982 AT4G26640.1 38.889 90 39 3 87 176 308 381 2.13e-12 67.8
MsaG041982 AT4G26640.1 49.153 59 26 2 87 145 138 192 1.72e-11 64.7
MsaG041982 AT4G26640.5 38.889 90 39 3 87 176 204 277 2.29e-12 67.4
MsaG041982 AT4G26640.5 49.153 59 26 2 87 145 34 88 1.82e-11 64.7
MsaG041982 AT4G26640.6 38.889 90 39 3 87 176 204 277 2.29e-12 67.4
MsaG041982 AT4G26640.6 49.153 59 26 2 87 145 34 88 1.82e-11 64.7
MsaG041982 AT4G26640.4 38.889 90 39 3 87 176 204 277 2.29e-12 67.4
MsaG041982 AT4G26640.4 49.153 59 26 2 87 145 34 88 1.82e-11 64.7
MsaG041982 AT4G26640.3 38.889 90 39 3 87 176 204 277 2.29e-12 67.4
MsaG041982 AT4G26640.3 49.153 59 26 2 87 145 34 88 1.82e-11 64.7
MsaG041982 AT2G25000.2 38.298 94 50 2 86 179 101 186 2.31e-12 65.9
MsaG041982 AT2G25000.4 38.298 94 50 2 86 179 101 186 2.31e-12 65.9
MsaG041982 AT4G26440.1 42.045 88 41 4 87 174 177 254 2.50e-12 67.4
MsaG041982 AT4G26440.1 48.333 60 28 1 87 146 371 427 1.89e-11 64.7
MsaG041982 AT2G25000.3 38.298 94 50 2 86 179 123 208 2.53e-12 65.9
MsaG041982 AT5G46350.1 37.363 91 47 3 87 175 182 264 2.67e-12 66.6
MsaG041982 AT3G01080.3 50.847 59 25 2 87 145 204 258 2.83e-12 66.6
MsaG041982 AT2G25000.1 38.298 94 50 2 86 179 144 229 3.54e-12 65.9
MsaG041982 AT3G01080.2 50.847 59 25 2 87 145 204 258 4.80e-12 66.6
MsaG041982 AT4G39410.1 38.202 89 52 1 63 151 198 283 5.26e-12 65.5
MsaG041982 AT5G56270.1 48.438 64 29 2 82 145 267 326 5.87e-12 66.6
MsaG041982 AT3G01080.1 50.847 59 25 2 87 145 166 220 6.01e-12 66.2
MsaG041982 AT4G04450.2 49.275 69 28 2 87 155 197 258 6.49e-12 65.9
MsaG041982 AT2G30250.1 52.632 57 24 1 89 145 329 382 7.03e-12 65.9
MsaG041982 AT1G62300.1 49.275 69 28 2 87 155 311 372 8.26e-12 65.9
MsaG041982 AT2G44745.1 47.458 59 28 1 87 145 144 199 1.00e-11 63.9
MsaG041982 AT4G04450.1 49.275 69 28 2 87 155 291 352 1.10e-11 65.5
MsaG041982 AT4G22070.4 44.048 84 42 2 62 145 274 352 1.16e-11 65.5
MsaG041982 AT4G22070.3 44.048 84 42 2 62 145 274 352 1.16e-11 65.5
MsaG041982 AT4G22070.2 44.048 84 42 2 62 145 274 352 1.16e-11 65.5
MsaG041982 AT4G22070.1 44.048 84 42 2 62 145 274 352 1.16e-11 65.5
MsaG041982 AT1G80590.1 29.333 150 84 3 89 237 86 214 1.41e-11 63.5
MsaG041982 AT5G41570.1 36.145 83 45 2 63 145 78 152 4.49e-11 61.2
MsaG041982 AT4G12020.10 49.153 59 26 2 87 145 467 521 5.88e-11 63.9
MsaG041982 AT4G12020.3 49.153 59 26 2 87 145 467 521 5.94e-11 63.9
MsaG041982 AT5G01900.1 45.763 59 32 0 89 147 111 169 6.00e-11 62.0
MsaG041982 AT4G12020.1 49.153 59 26 2 87 145 467 521 6.02e-11 63.5
MsaG041982 AT4G12020.9 49.153 59 26 2 87 145 467 521 6.02e-11 63.5
MsaG041982 AT4G12020.2 49.153 59 26 2 87 145 467 521 6.16e-11 63.5
MsaG041982 AT4G12020.4 49.153 59 26 2 87 145 467 521 6.22e-11 63.5
MsaG041982 AT4G12020.7 49.153 59 26 2 87 145 467 521 6.24e-11 63.5
MsaG041982 AT4G12020.6 49.153 59 26 2 87 145 467 521 6.28e-11 63.5
MsaG041982 AT4G12020.8 49.153 59 26 2 87 145 467 521 6.28e-11 63.5
MsaG041982 AT4G12020.5 49.153 59 26 2 87 145 400 454 6.48e-11 63.5
MsaG041982 AT2G38470.1 51.724 58 24 2 88 145 184 237 6.49e-11 63.2
MsaG041982 AT3G62340.1 46.667 60 29 1 87 146 117 173 7.30e-11 62.0
MsaG041982 AT5G15130.2 49.180 61 29 1 86 146 183 241 7.34e-11 63.2
MsaG041982 AT1G80840.1 38.298 94 48 3 76 166 131 217 7.78e-11 62.0
MsaG041982 AT5G15130.1 49.180 61 29 1 86 146 225 283 8.10e-11 62.8
MsaG041982 AT1G64000.1 45.763 59 29 1 87 145 113 168 8.43e-11 60.8
MsaG041982 AT1G68150.1 50.820 61 28 1 85 145 232 290 8.48e-11 62.4

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGAATTGGCAATAGAATT+TGG 0.147921 8:-14991308 None:intergenic
CTGCTTGTCACTATTGGATA+TGG 0.238656 8:+14991241 MsaT041982.1:CDS
TGAGTCTTGGACCTCACTTT+TGG 0.250506 8:-14989098 None:intergenic
ATCAAAATCCATTGGGGAAT+TGG 0.278398 8:-14991321 None:intergenic
GCATGTGTGTCTTCCTTTGT+AGG 0.282849 8:-14990937 None:intergenic
CATGATTGAAAATAATTTCA+TGG 0.317861 8:+14991178 MsaT041982.1:CDS
GAGCAAGTGAAGGTTTATTC+TGG 0.340277 8:+14989319 MsaT041982.1:CDS
ATGGGAGTTTCTTGTTAGAT+TGG 0.341676 8:-14990962 None:intergenic
AACATTAAGTGTGAAGAAAT+TGG 0.349506 8:+14988836 MsaT041982.1:CDS
AGTGAGACTGAGATAATTTC+AGG 0.355518 8:+14991284 MsaT041982.1:CDS
GGGAATTGGCAATAGAATTT+GGG 0.359861 8:-14991307 None:intergenic
TATGGACAAAAGGATATTCT+TGG 0.372931 8:+14989388 MsaT041982.1:CDS
GGTTATAGCTGGAGAAAGTA+TGG 0.376671 8:+14989370 MsaT041982.1:CDS
ATGAATTCTCATTGTTCAAA+TGG 0.381161 8:+14989070 MsaT041982.1:CDS
TGTGCTTGAACTTTGAGTCT+TGG 0.395293 8:-14989111 None:intergenic
TATAGAGATCAAAATCCATT+GGG 0.432731 8:-14991328 None:intergenic
TGGGAGTTTCTTGTTAGATT+GGG 0.445225 8:-14990961 None:intergenic
CTGGAGAAAGTATGGACAAA+AGG 0.452781 8:+14989378 MsaT041982.1:CDS
AAATAATTTCATGGAAAATG+AGG 0.454119 8:+14991187 MsaT041982.1:CDS
TCCCTCATTACCCTTTGCCA+TGG 0.480628 8:-14989020 None:intergenic
TGTGAAGAAATTGGTCTCAT+TGG 0.485916 8:+14988845 MsaT041982.1:CDS
AACCATGGCAAAGGGTAATG+AGG 0.498135 8:+14989018 MsaT041982.1:CDS
GAAATTATCTCAGTCTCACT+TGG 0.500825 8:-14991281 None:intergenic
ACAAACATGTGTCCAAGAAA+AGG 0.507877 8:+14989131 MsaT041982.1:CDS
CTGTTGAGGATGGTTATAGC+TGG 0.522098 8:+14989359 MsaT041982.1:CDS
TTCAGACCCTATTGATGGAG+AGG 0.523902 8:-14991126 None:intergenic
TTCTTGGAGCTAAGTTTCCA+AGG 0.535081 8:+14989404 MsaT041982.1:CDS
TTCTATTGCCAATTCCCCAA+TGG 0.537538 8:+14991313 MsaT041982.1:CDS
TTATAGAGATCAAAATCCAT+TGG 0.559577 8:-14991329 None:intergenic
TTCACTTGCTCTGTCCACTT+AGG 0.560248 8:-14989307 None:intergenic
GATATGGACAGCACTGGACT+AGG 0.571491 8:+14991257 MsaT041982.1:CDS
GACAGCAATAACCATGGCAA+AGG 0.573478 8:+14989009 MsaT041982.1:intron
ACAGCAATAACCATGGCAAA+GGG 0.573968 8:+14989010 MsaT041982.1:intron
TATTCTGGAACAGCAGCTGA+AGG 0.574098 8:+14989334 MsaT041982.1:CDS
ACCATGGCAAAGGGTAATGA+GGG 0.583479 8:+14989019 MsaT041982.1:CDS
TTGCTCTGTCCACTTAGGCA+CGG 0.602998 8:-14989302 None:intergenic
GCTGAAGGATCTGTTGAGGA+TGG 0.616817 8:+14989349 MsaT041982.1:CDS
CTATTGGATATGGACAGCAC+TGG 0.619978 8:+14991251 MsaT041982.1:CDS
AGCAGCTGAAGGATCTGTTG+AGG 0.621900 8:+14989345 MsaT041982.1:CDS
AAATTATCTCAGTCTCACTT+GGG 0.640870 8:-14991280 None:intergenic
AAATGGAAGTCCCAAAAGTG+AGG 0.645726 8:+14989087 MsaT041982.1:CDS
ATATGTGAGATAACCTACAA+AGG 0.649008 8:+14990924 MsaT041982.1:CDS
TAATGACATAAATTTCGACA+TGG 0.657601 8:+14991349 MsaT041982.1:CDS
TAAGTGGACAGAGCAAGTGA+AGG 0.673359 8:+14989309 MsaT041982.1:CDS
CTCATTGGTGAACTAATGCA+AGG 0.698867 8:+14988860 MsaT041982.1:CDS
CATTGAGACAGCAATAACCA+TGG 0.699108 8:+14989003 MsaT041982.1:intron
ATAGAGATCAAAATCCATTG+GGG 0.717113 8:-14991327 None:intergenic
TGCACACATAGACATGCACA+AGG 0.724252 8:+14990855 MsaT041982.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 14988827 14991409 14988827 ID=MsaG041982
Chr8 mRNA 14988827 14991409 14988827 ID=MsaT041982.1;Parent=MsaG041982
Chr8 exon 14988827 14988881 14988827 ID=MsaT041982.1.exon1;Parent=MsaT041982.1
Chr8 CDS 14988827 14988881 14988827 ID=cds.MsaT041982.1;Parent=MsaT041982.1
Chr8 exon 14989014 14989152 14989014 ID=MsaT041982.1.exon2;Parent=MsaT041982.1
Chr8 CDS 14989014 14989152 14989014 ID=cds.MsaT041982.1;Parent=MsaT041982.1
Chr8 exon 14989297 14989425 14989297 ID=MsaT041982.1.exon3;Parent=MsaT041982.1
Chr8 CDS 14989297 14989425 14989297 ID=cds.MsaT041982.1;Parent=MsaT041982.1
Chr8 exon 14990842 14991409 14990842 ID=MsaT041982.1.exon4;Parent=MsaT041982.1
Chr8 CDS 14990842 14991409 14990842 ID=cds.MsaT041982.1;Parent=MsaT041982.1
Gene Sequence

>MsaT041982.1

ATGAAAGAGAACATTAAGTGTGAAGAAATTGGTCTCATTGGTGAACTAATGCAAGCAATAACCATGGCAAAGGGTAATGAGGGAAAAATTAAGACAAACAATGTCAACATGATGAATTCTCATTGTTCAAATGGAAGTCCCAAAAGTGAGGTCCAAGACTCAAAGTTCAAGCACAAACATGTGTCCAAGAAAAGAAAAACCGTGCCTAAGTGGACAGAGCAAGTGAAGGTTTATTCTGGAACAGCAGCTGAAGGATCTGTTGAGGATGGTTATAGCTGGAGAAAGTATGGACAAAAGGATATTCTTGGAGCTAAGTTTCCAAGAGGATATTACAGATGCACACATAGACATGCACAAGGTTGTCTAGCTACTAAGCAAGTGCAAAAATCAGATGAAGATGAAATGATATGTGAGATAACCTACAAAGGAAGACACACATGCACCCAATCTAACAAGAAACTCCCATCAAAAACAAAGTTGAGATTTGGACAAAATAAATCACAATCCAATCAAAATATTCAACCCCAAGAAGAGAAAATACAACCACCTCAAGAAAAAAAATTCTCTTTTGACAACAAGGAGGATATATTTCAATCATTCAATTTTTCCTCTCCATCAATAGGGTCTGAAAATGAAGATAACAACATATTTTTAGAGACCATGATTGAAAATAATTTCATGGAAAATGAGGATAACAATATATTCTCAGAATCAAACATTTTCTGCTTGTCACTATTGGATATGGACAGCACTGGACTAGGCCCAAGTGAGACTGAGATAATTTCAGGCCCAAATTCTATTGCCAATTCCCCAATGGATTTTGATCTCTATAATGACATAAATTTCGACATGGATGATTTCTCTATCAACACACAAGAACTTTGCTATTGA
Protein sequence

>MsaT041982.1

MKENIKCEEIGLIGELMQAITMAKGNEGKIKTNNVNMMNSHCSNGSPKSEVQDSKFKHKHVSKKRKTVPKWTEQVKVYSGTAAEGSVEDGYSWRKYGQKDILGAKFPRGYYRCTHRHAQGCLATKQVQKSDEDEMICEITYKGRHTCTQSNKKLPSKTKLRFGQNKSQSNQNIQPQEEKIQPPQEKKFSFDNKEDIFQSFNFSSPSIGSENEDNNIFLETMIENNFMENEDNNIFSESNIFCLSLLDMDSTGLGPSETEIISGPNSIANSPMDFDLYNDINFDMDDFSINTQELCY*