AlfalfaGEDB | MsaG042016

M. sativa ssp. caerulea / MsaG042016

KEGG: K09060
GO: GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF07777.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042016	XP_013444711.1	95.735	422	18	0	1	422	1	422	0.0	832

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042016	sp\|Q99089\|CPRF1_PETCR	52.436	431	174	11	1	421	1	410	1.48e-131	387

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042016	tr\|A0A072TNE8\|A0A072TNE8_MEDTR	95.735	422	18	0	1	422	1	422	0.0	832

TFs/TRs:

Gene ID	Type	Classification
MsaG042016	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000023	MsaG042016	0.805434	2.262363e-49	3.464557e-47
MsaG000253	MsaG042016	0.819124	2.393378e-52	5.143892e-50
MsaG000621	MsaG042016	0.813765	3.740278e-51	7.009418e-49
MsaG000705	MsaG042016	0.858704	1.342569e-62	9.938259e-60
MsaG000867	MsaG042016	0.808655	4.742017e-50	7.839819e-48
MsaG001045	MsaG042016	0.820173	1.381964e-52	3.053574e-50
MsaG001092	MsaG042016	0.800270	2.606485e-48	3.545616e-46
MsaG001118	MsaG042016	0.800660	2.172186e-48	2.981138e-46
MsaG001128	MsaG042016	0.809665	2.888880e-50	4.893598e-48
MsaG001270	MsaG042016	0.804377	3.753172e-49	5.608459e-47
MsaG001618	MsaG042016	0.818651	3.060850e-52	6.497318e-50
MsaG002636	MsaG042016	0.806394	1.424064e-49	2.230753e-47
MsaG002804	MsaG042016	0.806147	1.604637e-49	2.498847e-47
MsaG002785	MsaG042016	0.822854	3.343313e-53	7.933915e-51
MsaG004091	MsaG042016	0.858452	1.595080e-62	1.169803e-59
MsaG004394	MsaG042016	0.801571	1.417958e-48	1.986410e-46
MsaG005038	MsaG042016	0.808865	4.279015e-50	7.110217e-48
MsaG005203	MsaG042016	0.803807	4.924167e-49	7.261643e-47
MsaG005226	MsaG042016	0.816776	8.068434e-52	1.631811e-49
MsaG005814	MsaG042016	0.818600	3.144110e-52	6.665047e-50
MsaG005940	MsaG042016	0.805800	1.896658e-49	2.929529e-47
MsaG006284	MsaG042016	0.805112	2.639437e-49	4.012006e-47
MsaG006512	MsaG042016	0.826772	4.022906e-54	1.062919e-51
MsaG007042	MsaG042016	0.812159	8.379617e-51	1.508600e-48
MsaG007043	MsaG042016	0.802751	8.127277e-49	1.169641e-46
MsaG007169	MsaG042016	0.816308	1.026302e-51	2.050883e-49
MsaG008424	MsaG042016	0.800269	2.607276e-48	3.546626e-46
MsaG008848	MsaG042016	0.815041	1.959832e-51	3.792359e-49
MsaG008874	MsaG042016	0.805014	2.766958e-49	4.196313e-47
MsaG009423	MsaG042016	0.810167	2.255476e-50	3.867340e-48
MsaG010307	MsaG042016	0.804483	3.567702e-49	5.344319e-47
MsaG010363	MsaG042016	0.800004	2.950299e-48	3.989509e-46
MsaG010472	MsaG042016	0.803611	5.404842e-49	7.934271e-47
MsaG010635	MsaG042016	0.816049	1.171886e-51	2.326333e-49
MsaG010604	MsaG042016	0.853870	3.457004e-61	2.145099e-58
MsaG010792	MsaG042016	0.823992	1.817105e-53	4.447070e-51
MsaG011100	MsaG042016	0.801924	1.200782e-48	1.695774e-46
MsaG011678	MsaG042016	0.825328	8.837019e-54	2.243088e-51
MsaG042016	MsaG042353	0.825697	7.231494e-54	1.854432e-51
MsaG042016	MsaG042720	0.810575	1.842851e-50	3.191504e-48
MsaG042016	MsaG043183	0.829519	8.828045e-55	2.519957e-52
MsaG042016	MsaG044630	0.817736	4.919520e-52	1.019832e-49
MsaG042016	MsaG044869	0.802613	8.676317e-49	1.244752e-46
MsaG042016	MsaG045129	0.817138	6.697645e-52	1.367312e-49
MsaG042016	MsaG046278	0.821019	8.854291e-53	2.000710e-50
MsaG042016	MsaG046860	0.827652	2.482422e-54	6.722551e-52
MsaG042016	MsaG046957	0.829952	6.936131e-55	2.004640e-52
MsaG042016	MsaG009146	0.815810	1.324304e-51	2.613025e-49
MsaG042016	MsaG006540	0.839776	2.389830e-57	9.263717e-55
MsaG042016	MsaG008699	0.800475	2.368793e-48	3.237300e-46
MsaG042016	MsaG006897	0.802498	9.159280e-49	1.310578e-46
MsaG042016	MsaG012745	0.816645	8.632363e-52	1.739894e-49
MsaG042016	MsaG011544	0.817730	4.933728e-52	1.022641e-49
MsaG042016	MsaG012608	0.805291	2.423215e-49	3.698566e-47
MsaG042016	MsaG015535	0.800253	2.627551e-48	3.572886e-46
MsaG042016	MsaG019406	0.800343	2.519398e-48	3.432841e-46
MsaG042016	MsaG018389	0.802704	8.311284e-49	1.194823e-46
MsaG042016	MsaG019826	0.831387	3.098578e-55	9.332116e-53
MsaG042016	MsaG017922	0.824457	1.414818e-53	3.506676e-51
MsaG042016	MsaG027471	0.812922	5.716883e-51	1.048988e-48
MsaG042016	MsaG024981	0.827891	2.176459e-54	5.933704e-52
MsaG042016	MsaG032293	0.814694	2.337677e-51	4.484117e-49
MsaG042016	MsaG031416	0.856781	4.956570e-62	3.416534e-59
MsaG042016	MsaG030964	0.827091	3.379075e-54	9.007874e-52
MsaG042016	MsaG033663	0.822740	3.554263e-53	8.408470e-51
MsaG042016	MsaG036285	0.808851	4.308606e-50	7.156972e-48
MsaG042016	MsaG038133	0.811584	1.116433e-50	1.981825e-48
MsaG042016	MsaG036344	0.802210	1.049541e-48	1.491836e-46
MsaG042016	MsaG043110	0.803450	5.836034e-49	8.535464e-47
MsaG013968	MsaG042016	0.802996	7.238341e-49	1.047615e-46
MsaG014135	MsaG042016	0.831899	2.320624e-55	7.095010e-53
MsaG014207	MsaG042016	0.821493	6.893538e-53	1.577261e-50
MsaG014471	MsaG042016	0.809447	3.215174e-50	5.418050e-48
MsaG014651	MsaG042016	0.828794	1.320945e-54	3.693543e-52
MsaG014655	MsaG042016	0.810982	1.506283e-50	2.634653e-48
MsaG016056	MsaG042016	0.804814	3.045310e-49	4.597036e-47
MsaG016116	MsaG042016	0.810229	2.186708e-50	3.755202e-48
MsaG016118	MsaG042016	0.807919	6.794234e-50	1.103574e-47
MsaG016159	MsaG042016	0.830694	4.578000e-55	1.351377e-52
MsaG016228	MsaG042016	0.845690	6.516920e-59	3.055120e-56
MsaG016483	MsaG042016	0.800652	2.180590e-48	2.992124e-46
MsaG017148	MsaG042016	0.847492	2.110649e-59	1.050785e-56
MsaG017568	MsaG042016	0.804403	3.705721e-49	5.540920e-47
MsaG018680	MsaG042016	0.803267	6.364791e-49	9.269598e-47
MsaG019016	MsaG042016	0.803425	5.904415e-49	8.630579e-47
MsaG021129	MsaG042016	0.846881	3.098528e-59	1.511192e-56
MsaG021131	MsaG042016	0.810042	2.397986e-50	4.099287e-48
MsaG021164	MsaG042016	0.806485	1.363191e-49	2.139995e-47
MsaG021165	MsaG042016	0.825441	8.311535e-54	2.116237e-51
MsaG021166	MsaG042016	0.806216	1.551962e-49	2.420934e-47
MsaG021168	MsaG042016	0.815219	1.789903e-51	3.479297e-49
MsaG021264	MsaG042016	0.818606	3.133427e-52	6.643587e-50
MsaG021270	MsaG042016	0.828020	2.026454e-54	5.544784e-52
MsaG021592	MsaG042016	0.817010	7.154614e-52	1.455773e-49
MsaG022767	MsaG042016	0.809359	3.357125e-50	5.644798e-48
MsaG023997	MsaG042016	0.830532	5.013647e-55	1.473139e-52
MsaG024126	MsaG042016	0.805467	2.225864e-49	3.411322e-47
MsaG025838	MsaG042016	0.836075	2.114692e-56	7.315353e-54
MsaG026764	MsaG042016	0.822545	3.943578e-53	9.280243e-51
MsaG028632	MsaG042016	0.839894	2.226841e-57	8.664622e-55
MsaG029029	MsaG042016	0.824474	1.402259e-53	3.477072e-51
MsaG029064	MsaG042016	0.810511	1.901918e-50	3.288719e-48
MsaG029621	MsaG042016	0.816923	7.481307e-52	1.518839e-49
MsaG030216	MsaG042016	0.828474	1.577444e-54	4.371689e-52
MsaG030455	MsaG042016	0.821843	5.727574e-53	1.322691e-50
MsaG030698	MsaG042016	0.801171	1.710272e-48	2.374315e-46
MsaG032401	MsaG042016	0.822095	5.010033e-53	1.164797e-50
MsaG033570	MsaG042016	0.821399	7.245836e-53	1.653693e-50
MsaG033588	MsaG042016	0.835353	3.215072e-56	1.088366e-53
MsaG034620	MsaG042016	0.802241	1.034279e-48	1.471183e-46
MsaG035166	MsaG042016	0.836391	1.758963e-56	6.143342e-54
MsaG036442	MsaG042016	0.801700	1.334070e-48	1.874409e-46
MsaG036580	MsaG042016	0.806008	1.715709e-49	2.662988e-47
MsaG037421	MsaG042016	0.809760	2.757022e-50	4.680958e-48
MsaG038325	MsaG042016	0.819003	2.548323e-52	5.459448e-50
MsaG038327	MsaG042016	0.817656	5.126678e-52	1.060582e-49
MsaG038520	MsaG042016	0.821949	5.412435e-53	1.253466e-50
MsaG038522	MsaG042016	0.826176	5.573230e-54	1.448327e-51
MsaG039393	MsaG042016	0.810352	2.057460e-50	3.543973e-48
MsaG039899	MsaG042016	0.812245	8.029507e-51	1.448624e-48
MsaG040311	MsaG042016	0.841861	6.826822e-58	2.826546e-55
MsaG041735	MsaG042016	0.809137	3.745782e-50	6.264947e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042016	MtrunA17_Chr8g0347741	95.735	422	18	0	1	422	1	422	0.0	832
MsaG042016	MtrunA17_Chr7g0275321	70.629	429	113	7	1	421	1	424	0.0	588
MsaG042016	MtrunA17_Chr5g0410531	35.492	386	199	15	1	347	1	375	4.85e-47	166
MsaG042016	MtrunA17_Chr8g0369161	33.498	406	197	16	1	354	1	385	1.82e-41	151
MsaG042016	MtrunA17_Chr8g0373571	35.376	359	190	11	1	348	1	328	3.65e-39	143

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042016	AT2G46270.1	52.927	427	149	16	1	421	1	381	1.13e-118	351
MsaG042016	AT2G46270.3	52.927	427	149	16	1	421	1	381	1.13e-118	351
MsaG042016	AT2G46270.2	51.054	427	134	15	1	421	1	358	1.49e-108	324
MsaG042016	AT2G46270.4	51.054	427	134	15	1	421	1	358	1.49e-108	324
MsaG042016	AT4G01120.1	43.529	425	172	14	1	422	1	360	1.79e-83	260
MsaG042016	AT2G35530.1	35.897	351	166	17	36	347	42	372	9.67e-40	147
MsaG042016	AT4G36730.2	34.857	350	164	14	1	348	1	288	4.81e-36	135
MsaG042016	AT1G32150.1	36.104	385	185	18	1	347	1	362	1.68e-35	135
MsaG042016	AT4G36730.1	34.000	350	169	14	1	348	1	290	1.47e-34	130
MsaG042016	AT5G15830.1	40.566	106	58	3	272	375	67	169	5.79e-11	62.0

Find 116 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCAGCTTTGCACCAGAATT+TGG	0.174496	8:+15395070	None:intergenic
TTATATCTTCAGGCATATTA+TGG	0.179056	8:-15397989	MsaT042016.1:intron
TGCATTGACTGCTGAGAATT+TGG	0.206598	8:-15395570	MsaT042016.1:CDS
GTTGGTGTCTCTATGCTTGT+AGG	0.281246	8:+15397257	None:intergenic
GTTGGACCTGTACTTTCTTC+AGG	0.296175	8:-15396363	MsaT042016.1:CDS
GATTGAAAATGCTACATTAA+AGG	0.302646	8:-15395498	MsaT042016.1:intron
ACCCCTCCATGTGAATAAAA+TGG	0.310681	8:+15397717	None:intergenic
TTTGGAAGCAGCAGTCTCTT+TGG	0.315745	8:+15396428	None:intergenic
ATTCGACGGGTTGGCTTGAT+TGG	0.332587	8:+15398771	None:intergenic
TGTGAGAGCAAACCAAATTC+TGG	0.337122	8:-15395082	MsaT042016.1:CDS
GAATGAATTGGCTGAAAATT+CGG	0.342200	8:-15395531	MsaT042016.1:CDS
ATCAGTGTTTCCAGATGATT+TGG	0.348179	8:+15397235	None:intergenic
ACTATGCTTACAGTTGTTAC+AGG	0.353749	8:+15398871	None:intergenic
TGTGGAGCACTGGTGGAAAT+AGG	0.361837	8:+15396306	None:intergenic
AAAGCTGGAAAACACTCAAC+TGG	0.369633	8:-15395233	MsaT042016.1:CDS
TCCTGATTGGGCTGCCATGC+AGG	0.378974	8:-15398737	MsaT042016.1:intron
TATATCTTCAGGCATATTAT+GGG	0.390466	8:-15397988	MsaT042016.1:intron
TGTGGTGGTCCCCACATGTA+TGG	0.390846	8:+15397905	None:intergenic
AGAGCTGCAGAGGAAGAAAA+TGG	0.398971	8:-15395109	MsaT042016.1:CDS
GCAGCAGCTGAGTTGAAATA+TGG	0.399898	8:+15397947	None:intergenic
TCCTCTGCAGCTCTATCATC+AGG	0.402104	8:+15395118	None:intergenic
CAGCCTATGATGCATCCATA+TGG	0.403301	8:-15397756	MsaT042016.1:CDS
TGAGAATTTGGCGCTGAAAT+CGG	0.404953	8:-15395558	MsaT042016.1:CDS
AGTAAGTTCTCTGTACTTAC+AGG	0.412972	8:+15395163	None:intergenic
ACAGGCCGAGGCTGAAGAAT+TGG	0.415387	8:-15395606	MsaT042016.1:intron
ACCTGCATGGCAGCCCAATC+AGG	0.417831	8:+15398736	None:intergenic
GGTGCATATGGCGGCCCATA+TGG	0.422943	8:+15397741	None:intergenic
GCCTCCTGAAGCTTGGATGC+AGG	0.426706	8:-15396268	MsaT042016.1:intron
GACGGGCTTGCAATGTCAAT+AGG	0.426918	8:-15397182	MsaT042016.1:CDS
ATCATCTGGAAACACTGATC+AGG	0.436322	8:-15397231	MsaT042016.1:CDS
GTTGCCATCGCTTCCATCGC+TGG	0.439957	8:+15396966	None:intergenic
AGAAGGCCTTTCAATCTTAA+CGG	0.439968	8:+15398897	None:intergenic
AAGATCCAGGCTTAGGAAGC+AGG	0.440032	8:-15396051	MsaT042016.1:intron
GAGACACCAACCAAATCATC+TGG	0.448010	8:-15397245	MsaT042016.1:CDS
ATGTTCACGTCTATCCTGAT+TGG	0.449162	8:-15398750	MsaT042016.1:CDS
TTCAACTCAGCTGCTGCTCC+TGG	0.457412	8:-15397941	MsaT042016.1:CDS
TGTTCACGTCTATCCTGATT+GGG	0.463031	8:-15398749	MsaT042016.1:CDS
AAACTTACAGCAGGCGAAGA+TGG	0.468175	8:+15397382	None:intergenic
GGCCCATATGGATGCATCAT+AGG	0.470629	8:+15397753	None:intergenic
GAAATCGGAAATGAATGAAT+TGG	0.472002	8:-15395543	MsaT042016.1:CDS
TTCAGGTATGACCACATCAC+TGG	0.473232	8:-15396346	MsaT042016.1:CDS
GGCAAAACTCCACAAGGTTG+TGG	0.479999	8:+15396288	None:intergenic
GGAAGCGATGGCAACACTAC+AGG	0.486034	8:-15396958	MsaT042016.1:CDS
TCATACCTGCTTCCTAAGCC+TGG	0.486648	8:+15396046	None:intergenic
GAATAAAATGGTGGTGCATA+TGG	0.490133	8:+15397729	None:intergenic
AGTACCTGCATCCAAGCTTC+AGG	0.494330	8:+15396264	None:intergenic
CTGCAAGAAGATCCAGGCTT+AGG	0.497217	8:-15396058	MsaT042016.1:CDS
TGGTTGTTGGTGTCCCATCC+CGG	0.497265	8:+15396796	None:intergenic
TTTATCATACCAGTGGTTGT+TGG	0.502643	8:+15396783	None:intergenic
TCCACAAGGTTGTGGAGCAC+TGG	0.503689	8:+15396296	None:intergenic
ATATAGGGGTCGAATTCAAA+TGG	0.506926	8:-15397415	MsaT042016.1:intron
GACGTGAACATTCGACGGGT+TGG	0.516747	8:+15398762	None:intergenic
TGGAACAAGGAAAAGAAGCC+GGG	0.517217	8:-15396814	MsaT042016.1:CDS
TCGTGCCAATTCTTCAGCCT+CGG	0.521120	8:+15395601	None:intergenic
TGCAGCTCTATCATCAGGAT+TGG	0.521412	8:+15395123	None:intergenic
ATTACTTGAATAGATGGAGA+AGG	0.522234	8:-15396480	MsaT042016.1:intron
ACAGTTGTTACAGGTGAAGA+AGG	0.523511	8:+15398880	None:intergenic
AGCCTATGATGCATCCATAT+GGG	0.523891	8:-15397755	MsaT042016.1:CDS
ATACATGTGGGGACCACCAC+AGG	0.528881	8:-15397903	MsaT042016.1:intron
TTACTTGAATAGATGGAGAA+GGG	0.530286	8:-15396479	MsaT042016.1:intron
CTCATGGGAAACAGCGAGGA+TGG	0.530777	8:-15398925	MsaT042016.1:CDS
GAACAAACCTCCGTGATAGC+TGG	0.533419	8:+15397120	None:intergenic
ATGGAACAAGGAAAAGAAGC+CGG	0.533912	8:-15396815	MsaT042016.1:CDS
GAAAATCCGTTAAGATTGAA+AGG	0.538090	8:-15398903	MsaT042016.1:CDS
GTGAATCTGCAAGAAGATCC+AGG	0.538433	8:-15396064	MsaT042016.1:CDS
GCTGCCACAGCATTAGCTCT+AGG	0.543381	8:+15395028	None:intergenic
AAATAGGAGAAGGGTTCCTC+AGG	0.546191	8:+15396322	None:intergenic
TGCTCCTCACCCATACATGT+GGG	0.547429	8:-15397915	MsaT042016.1:CDS
GATACTGAGGGTTCCAGCGA+TGG	0.548274	8:-15396979	MsaT042016.1:CDS
CAAGGAAAAGAAGCCGGGAT+GGG	0.549908	8:-15396809	MsaT042016.1:CDS
ACAAGGTTGTGGAGCACTGG+TGG	0.565799	8:+15396299	None:intergenic
ACCAGTGCTCCACAACCTTG+TGG	0.566848	8:-15396297	MsaT042016.1:CDS
TCATCTGGAAACACTGATCA+GGG	0.569446	8:-15397230	MsaT042016.1:CDS
GAAGCGATGGCAACACTACA+GGG	0.570248	8:-15396957	MsaT042016.1:CDS
GAGCTGAAAACCAGCTATCA+CGG	0.570429	8:-15397130	MsaT042016.1:CDS
TTTGAACGGCATTGACAAGA+GGG	0.571991	8:-15395191	MsaT042016.1:CDS
ACAAGGAAAAGAAGCCGGGA+TGG	0.577909	8:-15396810	MsaT042016.1:CDS
TATGGGTGAGGAGCATGACC+AGG	0.581544	8:+15397923	None:intergenic
GGTTCCAGCGATGGAAGCGA+TGG	0.582155	8:-15396970	MsaT042016.1:CDS
TCCTGATGATAGAGCTGCAG+AGG	0.584828	8:-15395119	MsaT042016.1:CDS
GATGGGACACCAACAACCAC+TGG	0.585041	8:-15396792	MsaT042016.1:intron
GGTCCCCACATGTATGGGTG+AGG	0.585719	8:+15397911	None:intergenic
GCACTGGTGGAAATAGGAGA+AGG	0.586444	8:+15396312	None:intergenic
GTGAGCTGAAACGTGAGAGG+AGG	0.593235	8:-15396100	MsaT042016.1:CDS
ATGCTCCTCACCCATACATG+TGG	0.594125	8:-15397916	MsaT042016.1:CDS
CAAAAGGTTGGTAACCTGCA+TGG	0.594851	8:+15398723	None:intergenic
GTGTTTCCAGATGATTTGGT+TGG	0.595146	8:+15397239	None:intergenic
GGGTCGAATTCAAATGGTCA+AGG	0.595761	8:-15397409	MsaT042016.1:CDS
GTTCCTCAGGTCCAGTGATG+TGG	0.595870	8:+15396335	None:intergenic
CTATGGAGCACTACCTGTGG+TGG	0.596426	8:+15397890	None:intergenic
CTTGCAATGTCAATAGGCAA+TGG	0.600080	8:-15397176	MsaT042016.1:CDS
AAACTTACAATGGCAACGGC+AGG	0.601671	8:+15397684	None:intergenic
GTGGTGGTCCCCACATGTAT+GGG	0.604143	8:+15397906	None:intergenic
GCAGCTGAGTTGAAATATGG+TGG	0.615300	8:+15397950	None:intergenic
CAATGCTATGCACAGACCAA+TGG	0.615349	8:-15396834	MsaT042016.1:intron
TATCAACAGTGTGGATACTG+AGG	0.618321	8:-15396992	MsaT042016.1:intron
GTCATACCTGAAGAAAGTAC+AGG	0.620947	8:+15396357	None:intergenic
GATAGACGTGAACATTCGAC+GGG	0.622023	8:+15398758	None:intergenic
ATGCACAGACCAATGGAACA+AGG	0.622555	8:-15396827	MsaT042016.1:intron
AAGCTGGAAAACACTCAACT+GGG	0.626485	8:-15395232	MsaT042016.1:CDS
GGATAGACGTGAACATTCGA+CGG	0.628969	8:+15398757	None:intergenic
AGCGTGAGCTGAAACGTGAG+AGG	0.634386	8:-15396103	MsaT042016.1:CDS
ACCTGCATCCAAGCTTCAGG+AGG	0.636392	8:+15396267	None:intergenic
ATGTAAACTTACAATGGCAA+CGG	0.640249	8:+15397680	None:intergenic
TGACCACATCACTGGACCTG+AGG	0.641825	8:-15396338	MsaT042016.1:CDS
CACTGGTGGAAATAGGAGAA+GGG	0.645035	8:+15396313	None:intergenic
ATCAACAGTGTGGATACTGA+GGG	0.652441	8:-15396991	MsaT042016.1:intron
TCAGGAGGCAAAACTCCACA+AGG	0.685943	8:+15396282	None:intergenic
GCTCCTCACCCATACATGTG+GGG	0.694056	8:-15397914	MsaT042016.1:CDS
AAATCCTAGAGCTAATGCTG+TGG	0.699726	8:-15395032	MsaT042016.1:CDS
TAAAATGGTGGTGCATATGG+CGG	0.709796	8:+15397732	None:intergenic
TTATCTCATGGGAAACAGCG+AGG	0.711063	8:-15398929	None:intergenic
AATGCTGAAAGTGCTGAGCG+TGG	0.716536	8:-15397152	MsaT042016.1:CDS
CCTCCATGTGAATAAAATGG+TGG	0.718697	8:+15397720	None:intergenic
AAGCGATGGCAACACTACAG+GGG	0.742066	8:-15396956	MsaT042016.1:intron
CTGAAAACCAGCTATCACGG+AGG	0.802626	8:-15397127	MsaT042016.1:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	15395021	15398944	15395021	ID=MsaG042016
Chr8	mRNA	15395021	15398944	15395021	ID=MsaT042016.1;Parent=MsaG042016
Chr8	exon	15395021	15395257	15395021	ID=MsaT042016.1.exon12;Parent=MsaT042016.1
Chr8	CDS	15395021	15395257	15395021	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15395499	15395624	15395499	ID=MsaT042016.1.exon11;Parent=MsaT042016.1
Chr8	CDS	15395499	15395624	15395499	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15396052	15396129	15396052	ID=MsaT042016.1.exon10;Parent=MsaT042016.1
Chr8	CDS	15396052	15396129	15396052	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15396269	15396489	15396269	ID=MsaT042016.1.exon9;Parent=MsaT042016.1
Chr8	CDS	15396269	15396489	15396269	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15396793	15396841	15396793	ID=MsaT042016.1.exon8;Parent=MsaT042016.1
Chr8	CDS	15396793	15396841	15396793	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15396957	15397005	15396957	ID=MsaT042016.1.exon7;Parent=MsaT042016.1
Chr8	CDS	15396957	15397005	15396957	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15397128	15397288	15397128	ID=MsaT042016.1.exon6;Parent=MsaT042016.1
Chr8	CDS	15397128	15397288	15397128	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15397390	15397431	15397390	ID=MsaT042016.1.exon5;Parent=MsaT042016.1
Chr8	CDS	15397390	15397431	15397390	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15397692	15397775	15397692	ID=MsaT042016.1.exon4;Parent=MsaT042016.1
Chr8	CDS	15397692	15397775	15397692	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15397904	15397999	15397904	ID=MsaT042016.1.exon3;Parent=MsaT042016.1
Chr8	CDS	15397904	15397999	15397904	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15398738	15398800	15398738	ID=MsaT042016.1.exon2;Parent=MsaT042016.1
Chr8	CDS	15398738	15398800	15398738	ID=cds.MsaT042016.1;Parent=MsaT042016.1
Chr8	exon	15398882	15398944	15398882	ID=MsaT042016.1.exon1;Parent=MsaT042016.1
Chr8	CDS	15398882	15398944	15398882	ID=cds.MsaT042016.1;Parent=MsaT042016.1

Gene Sequence

>MsaT042016.1

ATGGGAAACAGCGAGGATGGAAAATCCGTTAAGATTGAAAGGCCTTCTTCACCTGTAACAACTGATCAGACCAATCAAGCCAACCCGTCGAATGTTCACGTCTATCCTGATTGGGCTGCCATGCAGGCATATTATGGGCAAAGAGTCAACATTCCACCATATTTCAACTCAGCTGCTGCTCCTGGTCATGCTCCTCACCCATACATGTGGGGACCACCACAGCCTATGATGCATCCATATGGGCCGCCATATGCACCACCATTTTATTCACATGGAGGGGTTTATACTCATCCTGCCGTTGCCATTGGGTCGAATTCAAATGGTCAAGGAATTCCATCTTCGCCTGCTGCTGGGACACCTACAAGCATAGAGACACCAACCAAATCATCTGGAAACACTGATCAGGGTTTAATGAAAAAATTGAAAGGGTTTGACGGGCTTGCAATGTCAATAGGCAATGGCAATGCTGAAAGTGCTGAGCGTGGAGCTGAAAACCAGCTATCACGGAGTGTGGATACTGAGGGTTCCAGCGATGGAAGCGATGGCAACACTACAGGGACCAATGGAACAAGGAAAAGAAGCCGGGATGGGACACCAACAACCACTGATGGAGAAGGGAAAGCTGAGATGCAAGACAGTCAAGTTTCCAAAGAGACTGCTGCTTCCAAAAAGACGGTGTCAGTTATCGCAACCAGTGCTGCAGAAAAAATGGTTGGACCTGTACTTTCTTCAGGTATGACCACATCACTGGACCTGAGGAACCCTTCTCCTATTTCCACCAGTGCTCCACAACCTTGTGGAGTTTTGCCTCCTGAAGCTTGGATGCAGAATGAGCGTGAGCTGAAACGTGAGAGGAGGAAGCAGTCAAATCGTGAATCTGCAAGAAGATCCAGGCTTAGGAAGCAGGCCGAGGCTGAAGAATTGGCACGAAGAGTCGATGCATTGACTGCTGAGAATTTGGCGCTGAAATCGGAAATGAATGAATTGGCTGAAAATTCGGCGAAGCTGAAGATTGAAAATGCTACATTAAAGGAAAAGCTGGAAAACACTCAACTGGGACAAACAGAAGAGATAATTTTGAACGGCATTGACAAGAGGGCTACACCTGTAAGTACAGAGAACTTACTGTCAAGAGTTGATAATTCCAATCCTGATGATAGAGCTGCAGAGGAAGAAAATGGTTTCTGTGAGAGCAAACCAAATTCTGGTGCAAAGCTGCGTCAACTACTTGACACAAATCCTAGAGCTAATGCTGTGGCAGCTAGTTGA

Protein sequence

>MsaT042016.1

MGNSEDGKSVKIERPSSPVTTDQTNQANPSNVHVYPDWAAMQAYYGQRVNIPPYFNSAAAPGHAPHPYMWGPPQPMMHPYGPPYAPPFYSHGGVYTHPAVAIGSNSNGQGIPSSPAAGTPTSIETPTKSSGNTDQGLMKKLKGFDGLAMSIGNGNAESAERGAENQLSRSVDTEGSSDGSDGNTTGTNGTRKRSRDGTPTTTDGEGKAEMQDSQVSKETAASKKTVSVIATSAAEKMVGPVLSSGMTTSLDLRNPSPISTSAPQPCGVLPPEAWMQNERELKRERRKQSNRESARRSRLRKQAEAEELARRVDALTAENLALKSEMNELAENSAKLKIENATLKEKLENTQLGQTEEIILNGIDKRATPVSTENLLSRVDNSNPDDRAAEEENGFCESKPNSGAKLRQLLDTNPRANAVAAS*