AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042228 MtrunA17_Chr8g0350321 99.616 781 3 0 1 781 30 810 0.0 1612
MsaG042228 MtrunA17_Chr7g0246631 81.305 797 114 13 1 781 1 778 0.0 1280
MsaG042228 MtrunA17_Chr1g0203671 65.102 788 245 13 10 778 14 790 0.0 982
MsaG042228 MtrunA17_Chr2g0322731 55.526 751 275 13 48 780 59 768 0.0 787
MsaG042228 MtrunA17_Chr4g0024351 54.740 749 295 12 45 781 17 733 0.0 776
MsaG042228 MtrunA17_Chr6g0472471 52.406 769 336 13 29 777 7 765 0.0 764
MsaG042228 MtrunA17_Chr6g0481041 49.239 723 316 13 86 781 29 727 0.0 673
MsaG042228 MtrunA17_Chr2g0331581 45.690 696 327 11 90 780 143 792 0.0 629
MsaG042228 MtrunA17_Chr5g0410711 44.622 753 376 19 50 781 49 781 0.0 621
MsaG042228 MtrunA17_Chr5g0394111 46.800 500 249 7 285 777 157 646 9.42e-150 452
MsaG042228 MtrunA17_Chr5g0394111 46.154 156 78 3 51 206 3 152 1.29e-29 125
MsaG042228 MtrunA17_Chr7g0224451 43.400 500 206 11 80 570 10 441 3.89e-124 379
MsaG042228 MtrunA17_Chr2g0292731 37.475 491 287 11 295 777 70 548 4.28e-98 315
MsaG042228 MtrunA17_Chr2g0292741 36.605 489 289 11 295 777 62 535 9.91e-94 303
MsaG042228 MtrunA17_Chr7g0224481 41.688 391 160 8 80 463 10 339 2.06e-85 274
MsaG042228 MtrunA17_Chr7g0224491 47.352 321 136 9 466 779 1 295 7.62e-83 266
MsaG042228 MtrunA17_Chr2g0292761 30.323 620 345 18 165 777 1 540 1.34e-75 255
MsaG042228 MtrunA17_Chr5g0419821 53.543 127 58 1 81 206 28 154 5.66e-41 148
MsaG042228 MtrunA17_Chr2g0319671 32.946 258 132 4 290 514 19 268 2.90e-38 144
MsaG042228 MtrunA17_Chr2g0319701 39.004 241 127 7 547 781 1 227 1.30e-37 140
MsaG042228 MtrunA17_Chr2g0319681 36.250 240 137 5 547 781 1 229 5.43e-35 133
MsaG042228 MtrunA17_Chr1g0205981 35.349 215 104 5 141 347 1 188 4.05e-29 115
MsaG042228 MtrunA17_Chr2g0319691 42.478 113 61 1 402 514 1 109 1.29e-26 106
MsaG042228 MtrunA17_Chr7g0224461 48.780 123 51 3 662 779 1 116 7.91e-26 102
MsaG042228 MtrunA17_Chr2g0304191 36.641 131 82 1 297 426 1 131 3.81e-22 93.2
MsaG042228 MtrunA17_Chr8g0340411 21.756 763 413 27 89 777 15 667 2.35e-21 99.8
MsaG042228 MtrunA17_Chr7g0224431 35.976 164 59 5 312 473 1 120 3.99e-17 78.2
MsaG042228 MtrunA17_Chr3g0140351 21.730 497 295 17 346 777 205 672 1.81e-14 77.8
MsaG042228 MtrunA17_Chr2g0331131 21.687 498 295 17 346 777 203 671 1.53e-12 71.2
MsaG042228 MtrunA17_Chr1g0179581 34.884 86 56 0 87 172 26 111 1.91e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042228 AT4G00730.1 71.080 778 188 9 14 781 52 802 0.0 1095
MsaG042228 AT3G61150.1 67.636 791 219 15 11 781 34 807 0.0 1038
MsaG042228 AT4G04890.2 55.764 746 279 14 53 780 26 738 0.0 804
MsaG042228 AT4G04890.1 55.764 746 279 14 53 780 26 738 0.0 804
MsaG042228 AT4G21750.2 55.204 759 285 10 53 780 26 760 0.0 799
MsaG042228 AT4G21750.1 55.204 759 285 10 53 780 26 760 0.0 799
MsaG042228 AT4G21750.3 55.204 759 285 10 53 780 26 760 0.0 799
MsaG042228 AT4G21750.4 55.204 759 285 10 53 780 26 760 0.0 799
MsaG042228 AT1G05230.8 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.4 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.9 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.10 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.7 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.11 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.2 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.6 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.5 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.1 52.487 764 316 14 21 781 1 720 0.0 769
MsaG042228 AT1G05230.3 52.487 764 314 15 21 781 1 718 0.0 764
MsaG042228 AT5G52170.3 54.270 726 254 12 59 778 26 679 0.0 751
MsaG042228 AT5G52170.1 54.270 726 254 12 59 778 26 679 0.0 751
MsaG042228 AT5G52170.2 53.899 731 254 13 59 778 26 684 0.0 745
MsaG042228 AT4G00730.2 67.362 527 142 7 14 530 52 558 0.0 706
MsaG042228 AT1G73360.1 49.215 701 328 12 86 777 32 713 0.0 627
MsaG042228 AT1G05230.12 50.786 636 277 13 21 653 1 603 0.0 615
MsaG042228 AT1G17920.2 46.759 725 327 17 71 781 6 685 0.0 612
MsaG042228 AT1G17920.1 46.759 725 327 17 71 781 6 685 0.0 612
MsaG042228 AT2G32370.1 43.036 718 340 11 80 781 60 724 0.0 576
MsaG042228 AT1G79840.1 40.609 788 386 15 19 780 14 745 0.0 565
MsaG042228 AT1G79840.3 42.796 701 339 12 86 780 43 687 0.0 564
MsaG042228 AT1G79840.6 42.796 701 339 12 86 780 43 687 0.0 564
MsaG042228 AT1G79840.2 40.377 795 392 15 12 780 36 774 0.0 563
MsaG042228 AT5G46880.3 42.162 740 373 19 71 777 89 806 3.12e-179 535
MsaG042228 AT5G46880.1 42.162 740 373 19 71 777 89 806 3.12e-179 535
MsaG042228 AT5G46880.2 42.162 740 373 19 71 777 89 806 3.12e-179 535
MsaG042228 AT4G25530.1 39.360 719 377 13 68 781 22 686 1.15e-163 490
MsaG042228 AT1G79840.4 39.910 664 323 13 12 649 7 620 1.06e-156 470
MsaG042228 AT4G17710.1 38.952 706 339 17 81 777 81 703 3.17e-146 446
MsaG042228 AT3G03260.1 36.878 743 389 21 68 779 3 696 8.16e-144 439
MsaG042228 AT5G17320.1 35.772 738 421 20 71 780 6 718 1.58e-130 405
MsaG042228 AT1G34650.1 34.746 708 420 17 93 780 23 708 6.11e-120 377
MsaG042228 AT1G79840.5 37.823 542 263 11 12 528 7 499 1.96e-108 341
MsaG042228 AT3G03260.2 36.252 571 312 16 68 621 3 538 3.30e-106 336
MsaG042228 AT5G07260.1 28.369 564 317 17 152 710 10 491 3.17e-56 202
MsaG042228 AT5G07260.2 28.114 562 316 16 152 710 10 486 4.77e-56 201
MsaG042228 AT4G26920.1 23.110 463 254 16 297 749 72 442 9.57e-23 102
MsaG042228 AT4G32880.1 23.062 503 291 19 340 777 199 670 1.21e-12 72.0
MsaG042228 AT1G30490.1 37.143 105 51 3 74 163 6 110 1.55e-12 72.0

Find 214 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGTGTTCTTCGATTT+TGG 0.132742 8:+18891999 None:intergenic
CCAAGCTCTAAACTTGAATT+TGG 0.182533 8:+18891605 None:intergenic
GATAACTTGGATGGTGTTTC+TGG 0.199972 8:-18892280 MsaT042228.1:CDS
GTTATATCTAATGGAATAAA+TGG 0.214668 8:-18890903 MsaT042228.1:CDS
TCCAATCTTTGTTTCTCATC+TGG 0.219888 8:+18892070 None:intergenic
TTTGAAACGCCACAGTTAAA+AGG 0.265578 8:+18886889 None:intergenic
GCACTTGCTGGAAAGTTTCT+AGG 0.281757 8:-18891647 MsaT042228.1:CDS
AAAGATTGGAGCTTAGTAAA+AGG 0.282647 8:-18892057 MsaT042228.1:CDS
CACAAACTAATGACCCTCTT+TGG 0.295700 8:-18891367 MsaT042228.1:CDS
GAGCTGAGAATATGTCTATA+AGG 0.296096 8:-18891796 MsaT042228.1:CDS
GCCATCGTCCCTGACGGTCC+TGG 0.296803 8:-18886978 MsaT042228.1:CDS
TTTGTGTTATAGTTTGTTTA+AGG 0.305442 8:-18892104 MsaT042228.1:intron
AGCAGCATGCAGAAGGTGTT+TGG 0.312158 8:-18890605 MsaT042228.1:CDS
GCTTAGTAAAAGGCTTTGTT+TGG 0.322328 8:-18892047 MsaT042228.1:CDS
CATGGGAATTATGAGCAAAA+TGG 0.323679 8:-18892346 MsaT042228.1:CDS
ACAAATGAAGTTATATCTAA+TGG 0.323910 8:-18890912 MsaT042228.1:CDS
AAACAAGACAAGTTAAGTTT+TGG 0.327392 8:-18892024 MsaT042228.1:CDS
TGCATTGGTTTGAAACCTAA+TGG 0.330001 8:-18891278 MsaT042228.1:CDS
GGCCCTCGTCGAGACTCTTA+TGG 0.331651 8:-18891204 MsaT042228.1:CDS
CGCCTCGCCGCCTCCATTTG+CGG 0.333322 8:+18886926 None:intergenic
GATAGAGTTTGTGCACTTGC+TGG 0.335931 8:-18891659 MsaT042228.1:CDS
TGTGCTATCGCCTTTGGTTC+CGG 0.337850 8:-18890658 MsaT042228.1:CDS
CTAATGACCCTCTTTGGATT+AGG 0.339140 8:-18891361 MsaT042228.1:CDS
AACAGTCTTTGAGGCGAAAC+CGG 0.340570 8:+18887921 None:intergenic
TGAACTTGCTTTGGCTGCTA+TGG 0.341605 8:-18891408 MsaT042228.1:CDS
CCAATCTTTGTTTCTCATCT+GGG 0.343195 8:+18892071 None:intergenic
TTCAAATAGACAAACATCTA+TGG 0.348262 8:-18891474 MsaT042228.1:CDS
TATGTTTCTTGAACTTGCTT+TGG 0.349553 8:-18891417 MsaT042228.1:CDS
ACGATCCCTGGTGGCTCGCC+TGG 0.350440 8:+18887972 None:intergenic
AGAAGTTGTTTGTCATTCGC+TGG 0.361029 8:+18888096 None:intergenic
TCTTGTTACTTCACCAGCTT+TGG 0.366038 8:-18892564 MsaT042228.1:CDS
GGTTAAGCTTGTTCCATTTG+TGG 0.368581 8:+18888048 None:intergenic
ACTTCATGTGCTATCGCCTT+TGG 0.371763 8:-18890664 MsaT042228.1:CDS
TGGGGTTTGGTGCGTCTCGT+TGG 0.382984 8:-18888464 MsaT042228.1:CDS
GTCTCGAGAGGGAGCGGCAG+AGG 0.383923 8:+18888395 None:intergenic
ATAGATGTTTGTCTATTTGA+AGG 0.389308 8:+18891476 None:intergenic
AGGAAATTTACCTCGCGAAC+CGG 0.392120 8:+18890639 None:intergenic
ATGAAGCAAACAAATATTGA+TGG 0.395849 8:-18892394 MsaT042228.1:intron
TCTGAGGCCTCAAGGGAAAC+TGG 0.398976 8:-18891248 MsaT042228.1:CDS
CCACCGCAATTCGAACAAAT+AGG 0.409469 8:+18891758 None:intergenic
GCGGCGAGGCGCGTGTTAGC+GGG 0.409750 8:-18886914 MsaT042228.1:CDS
CTTTGGATTAGGAGTATAGA+AGG 0.410489 8:-18891350 MsaT042228.1:CDS
GGAATAAATGGAACAAGAAA+TGG 0.416572 8:-18890891 MsaT042228.1:CDS
AGCGGCAGAGGACATTAATA+TGG 0.417806 8:+18888407 None:intergenic
ATTACCTTGGAGTAACCATT+TGG 0.419128 8:+18890480 None:intergenic
ATTAGATATAACTTCATTTG+TGG 0.423746 8:+18890914 None:intergenic
ACCTCTGTTGAGCTCAGGTA+TGG 0.428362 8:-18888484 MsaT042228.1:CDS
ACAATTGTACTAGACTCAAT+TGG 0.428686 8:-18891864 MsaT042228.1:intron
GAAGAGGAACAAAGTAGATC+TGG 0.428814 8:-18892307 MsaT042228.1:CDS
TTAGGAGTATAGAAGGAGGA+AGG 0.429115 8:-18891343 MsaT042228.1:CDS
GCAGCATGCAGAAGGTGTTT+GGG 0.431911 8:-18890604 MsaT042228.1:CDS
CCATCGTCCCTGACGGTCCT+GGG 0.432268 8:-18886977 MsaT042228.1:CDS
AAGGAGACACAATGGCTAAA+GGG 0.434268 8:+18891495 None:intergenic
TTGAGCTCAGGTATGGGGTT+TGG 0.434561 8:-18888477 MsaT042228.1:CDS
CACTGTTTCAACTGATTCCA+CGG 0.436432 8:+18886833 None:intergenic
AATTGAGAATGCGAGGTTGA+AGG 0.436963 8:-18891690 MsaT042228.1:CDS
GAAATCTCGCCGATCATAGC+TGG 0.437015 8:+18891734 None:intergenic
GTGCCTCAACGGTCCACAAA+TGG 0.441844 8:-18888061 MsaT042228.1:CDS
AATGGCGATGCAACCGCAAA+TGG 0.441940 8:-18886939 MsaT042228.1:CDS
GCCTCAGAAACAAAACCATT+AGG 0.446780 8:+18891263 None:intergenic
GACATTCCTGAACTAAAATC+AGG 0.446855 8:+18891524 None:intergenic
GGGGTTTGGTGCGTCTCGTT+GGG 0.449682 8:-18888463 MsaT042228.1:CDS
CCGCGTGACCCAGGACCGTC+AGG 0.450711 8:+18886969 None:intergenic
CCAAATTCAAGTTTAGAGCT+TGG 0.452576 8:-18891605 MsaT042228.1:CDS
GGCGGCGAGGCGCGTGTTAG+CGG 0.455890 8:-18886915 MsaT042228.1:CDS
AACATTGATTTAGCCAAAGC+TGG 0.456150 8:+18892551 None:intergenic
CGGTGATATCTCTTCTTCCT+AGG 0.457526 8:+18892229 None:intergenic
AGCCACGTCGGTGTGGCTCC+CGG 0.460196 8:-18887940 MsaT042228.1:CDS
ACATCTTCGTCTACATTACC+AGG 0.462223 8:+18888026 None:intergenic
AGGACATTCACTCCTTGCAT+TGG 0.464695 8:-18891293 MsaT042228.1:CDS
TTCGCGAGGTAAATTTCCTT+AGG 0.465241 8:-18890635 MsaT042228.1:CDS
CCCAGATGAGAAACAAAGAT+TGG 0.465465 8:-18892071 MsaT042228.1:CDS
GATACCATCAGAGAAACTTC+TGG 0.466240 8:-18890561 MsaT042228.1:CDS
TTCTCTAGCCCGCGTGACCC+AGG 0.466669 8:+18886960 None:intergenic
GAATTTGGTAACGAAGAAAT+CGG 0.466807 8:+18891620 None:intergenic
CGAAAGAGCGTCGATGATCC+AGG 0.466859 8:-18887990 MsaT042228.1:CDS
CCTCTGTTGAGCTCAGGTAT+GGG 0.468963 8:-18888483 MsaT042228.1:CDS
CTTTGTTTCTCATCTGGGTG+AGG 0.471059 8:+18892076 None:intergenic
TGCAGAAGGTGTTTGGGCTG+TGG 0.473907 8:-18890598 MsaT042228.1:CDS
AGCTGAGAATATGTCTATAA+GGG 0.474540 8:-18891795 MsaT042228.1:CDS
AACAACGAAAGAATGCCATT+TGG 0.477954 8:-18892605 None:intergenic
GAAGGAGACACAATGGCTAA+AGG 0.478406 8:+18891494 None:intergenic
TTGAAACGCCACAGTTAAAA+GGG 0.478927 8:+18886890 None:intergenic
GTCAGGGACGATGGCGAAGC+CGG 0.479104 8:+18886986 None:intergenic
ATGACAAACAACTTCTGTGC+CGG 0.479329 8:-18888089 MsaT042228.1:CDS
GCTGCGCTCAACACGATCCC+TGG 0.483232 8:+18887960 None:intergenic
TTTGGCTGCTATGGATGAGT+TGG 0.483619 8:-18891399 MsaT042228.1:CDS
AACTGTAGGAAGCTTCCCTC+TGG 0.485745 8:-18890522 MsaT042228.1:CDS
TAGATCTGGTAGTGATAACT+TGG 0.486150 8:-18892293 MsaT042228.1:CDS
AGTTTAGAGCTTGGTGTTGG+AGG 0.487497 8:-18891596 MsaT042228.1:CDS
CGAAAGGCTCCGTAGCGAAT+GGG 0.491168 8:-18887883 MsaT042228.1:CDS
GCACTCGCACTGGCGTTGTA+AGG 0.492699 8:+18888434 None:intergenic
TTCATGACAACATGCATAGC+TGG 0.492736 8:+18887044 None:intergenic
GGGTCACGCGGGCTAGAGAA+TGG 0.497914 8:-18886957 MsaT042228.1:CDS
GAATGGGATATACTCTCAAA+TGG 0.503595 8:-18887867 MsaT042228.1:CDS
CAAACATCTATGGTGACTAC+TGG 0.504285 8:-18891464 MsaT042228.1:CDS
TGACAAACAACTTCTGTGCC+GGG 0.505714 8:-18888088 MsaT042228.1:CDS
ACAGTCTTTGAGGCGAAACC+GGG 0.506478 8:+18887922 None:intergenic
CTCTGTTGAGCTCAGGTATG+GGG 0.507146 8:-18888482 MsaT042228.1:CDS
TGCCGGGGTCTGTGCCTCAA+CGG 0.507397 8:-18888072 MsaT042228.1:CDS
GTAGTATCAACATGCTACTC+TGG 0.510426 8:+18887123 None:intergenic
CTAAAATCAGGTAATGTTGA+AGG 0.511223 8:+18891536 None:intergenic
TAGGAGTATAGAAGGAGGAA+GGG 0.511252 8:-18891342 MsaT042228.1:CDS
CGTCGAGACTCTTATGGACT+CGG 0.512419 8:-18891198 MsaT042228.1:intron
ATATGGCAAGGCACTCGCAC+TGG 0.516470 8:+18888424 None:intergenic
TACTGTCATCATCAATAGCT+TGG 0.517600 8:-18891225 MsaT042228.1:CDS
GGTGGAACATACAGAATATG+AGG 0.517671 8:-18888532 MsaT042228.1:CDS
TCAAGTTTAGAGCTTGGTGT+TGG 0.517962 8:-18891599 MsaT042228.1:CDS
AACAGATTCAAGAGCTTGAA+AGG 0.519002 8:-18892195 MsaT042228.1:intron
AGAGTGGTCTCGAGAGGGAG+CGG 0.519414 8:+18888389 None:intergenic
TCAAGCTCTTGAATCTGTTG+AGG 0.520625 8:+18892199 None:intergenic
GATGCAACCGCAAATGGAGG+CGG 0.528418 8:-18886933 MsaT042228.1:CDS
AAATCGAAGAACACAAATGA+AGG 0.532956 8:-18891996 MsaT042228.1:intron
TATCAACATGCTACTCTGGT+TGG 0.534209 8:+18887127 None:intergenic
TACGTGGCATTACTACCATC+CGG 0.534267 8:-18887005 MsaT042228.1:CDS
TGAACCATGAGGAATATTTG+AGG 0.534711 8:-18891313 MsaT042228.1:CDS
ACGAAAGGCTCCGTAGCGAA+TGG 0.534843 8:-18887884 MsaT042228.1:CDS
AGAAAATCAAACAGTCTTTG+AGG 0.537109 8:+18887912 None:intergenic
GTCGCTTGTTGTCTATGCGC+CGG 0.538678 8:-18887075 MsaT042228.1:CDS
TATAGACCTCTGTTGAGCTC+AGG 0.541054 8:-18888489 MsaT042228.1:CDS
AAATCAATGTTCAACTCACC+TGG 0.542999 8:-18892539 MsaT042228.1:CDS
TGATGAATTTGCAGGTGACA+TGG 0.545053 8:-18888554 MsaT042228.1:intron
GGCATGAGAACAATTTGCTT+AGG 0.546874 8:-18891838 MsaT042228.1:CDS
TCAACAGAGGTCTATACAGC+TGG 0.547015 8:+18888496 None:intergenic
GAAGTTGTTTGTCATTCGCT+GGG 0.547175 8:+18888097 None:intergenic
CATGCTGCTTGCAAAACCTA+AGG 0.548250 8:+18890619 None:intergenic
AATCCTATTTGTTCGAATTG+CGG 0.552002 8:-18891761 MsaT042228.1:CDS
ATTGCTAAAGGACAAGATCA+TGG 0.552765 8:-18887819 MsaT042228.1:CDS
CCTGACGGTCCTGGGTCACG+CGG 0.554312 8:-18886969 MsaT042228.1:CDS
CCCAGGACCGTCAGGGACGA+TGG 0.554778 8:+18886977 None:intergenic
CTGTAGGAAGACTATTCACA+AGG 0.558656 8:+18886865 None:intergenic
GCCTTTGGTTCCGGTTCGCG+AGG 0.561467 8:-18890649 MsaT042228.1:CDS
GGCTTCGCCATCGTCCCTGA+CGG 0.566065 8:-18886984 MsaT042228.1:CDS
GACCGTTGAGGCACAGACCC+CGG 0.570884 8:+18888070 None:intergenic
CGTGTTGAGCGCAGCCACGT+CGG 0.572624 8:-18887952 MsaT042228.1:CDS
GATGATCCAGGCGAGCCACC+AGG 0.573378 8:-18887978 MsaT042228.1:CDS
CGGCGGCGATTCAGCTTACG+TGG 0.573596 8:-18887021 MsaT042228.1:CDS
GAAACATGTATAGACGAAGC+GGG 0.574467 8:-18887098 MsaT042228.1:CDS
ACCTCGCGAACCGGAACCAA+AGG 0.576063 8:+18890648 None:intergenic
AGATATAACTTCATTTGTGG+AGG 0.576220 8:+18890917 None:intergenic
AGCACCAGAAGTTTCTCTGA+TGG 0.578112 8:+18890557 None:intergenic
TATGGATGAGTTGGTTAAGA+TGG 0.580312 8:-18891390 MsaT042228.1:CDS
AGAAACATGTATAGACGAAG+CGG 0.580422 8:-18887099 MsaT042228.1:CDS
GTTCCATTTGTGGACCGTTG+AGG 0.580922 8:+18888058 None:intergenic
TTTGAGAAGAAGTAGAGAAG+AGG 0.581124 8:-18892323 MsaT042228.1:CDS
ACAAGTGCAAGTGATAATCC+AGG 0.581955 8:+18892521 None:intergenic
TGCGGTGGTCCAGCTATGAT+CGG 0.583098 8:-18891743 MsaT042228.1:CDS
GATGGACAAGAAGATGTGAA+TGG 0.584326 8:-18892376 MsaT042228.1:CDS
GAGGTTCTTGCAATGATACA+AGG 0.584664 8:+18890936 None:intergenic
TGGATTAGGAGTATAGAAGG+AGG 0.585727 8:-18891347 MsaT042228.1:CDS
TGCATAGCTGGAATATCGAC+CGG 0.586168 8:+18887056 None:intergenic
ATATGAGCCATCTCTTGCAT+AGG 0.588298 8:+18887840 None:intergenic
TGTCTCACAGCAATAACAGC+AGG 0.590368 8:-18888146 MsaT042228.1:intron
GGCGATGCAACCGCAAATGG+AGG 0.590559 8:-18886936 MsaT042228.1:CDS
GGTTGAGAGAAAGAACCAAA+TGG 0.592147 8:+18892590 None:intergenic
GAGATGGCTCATATTGCTAA+AGG 0.593426 8:-18887831 MsaT042228.1:CDS
CAGCAATAACAGCAGGAGGA+AGG 0.594087 8:-18888139 MsaT042228.1:CDS
AGAGTATATCCCATTCGCTA+CGG 0.595046 8:+18887874 None:intergenic
ATGTCCTCAAATATTCCTCA+TGG 0.596418 8:+18891309 None:intergenic
GCTATGCATGTTGTCATGAA+CGG 0.597242 8:-18887041 MsaT042228.1:CDS
TGATATCTCTTCTTCCTAGG+TGG 0.601999 8:+18892232 None:intergenic
CCTATTTGTTCGAATTGCGG+TGG 0.602780 8:-18891758 MsaT042228.1:CDS
TGGTGGTCCTATGCAAGAGA+TGG 0.604536 8:-18887847 MsaT042228.1:CDS
GCTCCCTCTCGAGACCACTC+TGG 0.605199 8:-18888387 MsaT042228.1:intron
CTATACTCCTAATCCAAAGA+GGG 0.605820 8:+18891354 None:intergenic
AAAGATAACTTACACTGGCA+CGG 0.606115 8:+18887778 None:intergenic
GGGACGATGGCGAAGCCGGA+TGG 0.606268 8:+18886990 None:intergenic
CAGAGGACATTAATATGGCA+AGG 0.607435 8:+18888412 None:intergenic
TCTATACTCCTAATCCAAAG+AGG 0.610543 8:+18891353 None:intergenic
ACAGAGGTCTATACAGCTGG+TGG 0.612963 8:+18888499 None:intergenic
ATCTTCGAATTGAGAATGCG+AGG 0.613235 8:-18891697 MsaT042228.1:CDS
TGTACTAGACTCAATTGGAA+AGG 0.615468 8:-18891859 MsaT042228.1:intron
ATGATCCAGGCGAGCCACCA+GGG 0.616138 8:-18887977 MsaT042228.1:CDS
TATGCCAAATGGTTACTCCA+AGG 0.620630 8:-18890484 MsaT042228.1:intron
GTTGTGCAAGATATGCCAAA+TGG 0.621690 8:-18890495 MsaT042228.1:CDS
TTAGGTTTCAAACCAATGCA+AGG 0.623981 8:+18891281 None:intergenic
ATACCAGAGTGGTCTCGAGA+GGG 0.629439 8:+18888384 None:intergenic
AACCGCAAATGGAGGCGGCG+AGG 0.633084 8:-18886928 MsaT042228.1:CDS
GATGAATTTGCAGGTGACAT+GGG 0.633419 8:-18888553 MsaT042228.1:intron
TCTATTTGAAGGAGACACAA+TGG 0.633437 8:+18891487 None:intergenic
CTGACGGTCCTGGGTCACGC+GGG 0.634906 8:-18886968 MsaT042228.1:CDS
GATGTGAATGGATCAATGCA+TGG 0.635991 8:-18892364 MsaT042228.1:CDS
GACAGTACCAGTTTCCCTTG+AGG 0.636068 8:+18891241 None:intergenic
ATGTGAATGGATCAATGCAT+GGG 0.636948 8:-18892363 MsaT042228.1:CDS
CATACCAGAGTGGTCTCGAG+AGG 0.638246 8:+18888383 None:intergenic
ATCTTGCACAACACAACCAG+AGG 0.640454 8:+18890506 None:intergenic
TGTCTATAAGGGATGCAATG+AGG 0.645251 8:-18891784 MsaT042228.1:CDS
GTTGGAGGAAACAACAACAA+TGG 0.647127 8:-18891581 MsaT042228.1:CDS
GCGCTCAACACGATCCCTGG+TGG 0.649684 8:+18887963 None:intergenic
TCTGGTAGTGATAACTTGGA+TGG 0.650981 8:-18892289 MsaT042228.1:CDS
CTCACAGCAATAACAGCAGG+AGG 0.659474 8:-18888143 MsaT042228.1:intron
TGAGCGCAGCCACGTCGGTG+TGG 0.662518 8:-18887947 MsaT042228.1:CDS
GACAAACAACTTCTGTGCCG+GGG 0.664289 8:-18888087 MsaT042228.1:CDS
GATCGGCGAGATTTCACTCG+AGG 0.666622 8:-18891726 MsaT042228.1:CDS
AACCGGGAGCCACACCGACG+TGG 0.667720 8:+18887938 None:intergenic
CATCTTCGTCTACATTACCA+GGG 0.669013 8:+18888027 None:intergenic
GAATCTGTTGAGGAGTGTGT+CGG 0.670166 8:+18892209 None:intergenic
TTGGTAACGAAGAAATCGGA+CGG 0.672520 8:+18891624 None:intergenic
CGCGTGACCCAGGACCGTCA+GGG 0.673759 8:+18886970 None:intergenic
GAATTTGCAGGTGACATGGG+TGG 0.674517 8:-18888550 MsaT042228.1:intron
AAATGGAACAAGCTTAACCC+TGG 0.676038 8:-18888044 MsaT042228.1:CDS
TGGGATATACTCTCAAATGG+TGG 0.680739 8:-18887864 MsaT042228.1:CDS
GTCCATAAGAGTCTCGACGA+GGG 0.680758 8:+18891202 None:intergenic
ATGCTGATGATAAACCACCT+AGG 0.683804 8:-18892246 MsaT042228.1:CDS
AAACATGTATAGACGAAGCG+GGG 0.692092 8:-18887097 MsaT042228.1:CDS
GTAATGTAGACGAAGATGTG+AGG 0.695572 8:-18888022 MsaT042228.1:CDS
TAATGTAGACGAAGATGTGA+GGG 0.696882 8:-18888021 MsaT042228.1:CDS
GCTGGTGAAGTAACAAGACG+AGG 0.699889 8:+18892569 None:intergenic
TCTTGCACAACACAACCAGA+GGG 0.701438 8:+18890507 None:intergenic
CCCATACCTGAGCTCAACAG+AGG 0.707512 8:+18888483 None:intergenic
TCCTACAGCAAAACTCACCG+TGG 0.709297 8:-18886850 MsaT042228.1:CDS
GAGTTAGAGTCATACCAGAG+TGG 0.711149 8:+18888373 None:intergenic
ATGCATGTTGTCATGAACGG+CGG 0.722395 8:-18887038 MsaT042228.1:CDS
AGTCCATAAGAGTCTCGACG+AGG 0.733323 8:+18891201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 18886773 18892615 18886773 ID=MsaG042228
Chr8 mRNA 18886773 18892615 18886773 ID=MsaT042228.1;Parent=MsaG042228
Chr8 exon 18886773 18887159 18886773 ID=MsaT042228.1.exon9;Parent=MsaT042228.1
Chr8 CDS 18886773 18887159 18886773 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18887791 18888158 18887791 ID=MsaT042228.1.exon8;Parent=MsaT042228.1
Chr8 CDS 18887791 18888158 18887791 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18888388 18888562 18888388 ID=MsaT042228.1.exon7;Parent=MsaT042228.1
Chr8 CDS 18888388 18888562 18888388 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18890485 18890697 18890485 ID=MsaT042228.1.exon6;Parent=MsaT042228.1
Chr8 CDS 18890485 18890697 18890485 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18890878 18890979 18890878 ID=MsaT042228.1.exon5;Parent=MsaT042228.1
Chr8 CDS 18890878 18890979 18890878 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18891199 18891873 18891199 ID=MsaT042228.1.exon4;Parent=MsaT042228.1
Chr8 CDS 18891199 18891873 18891199 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18891997 18892114 18891997 ID=MsaT042228.1.exon3;Parent=MsaT042228.1
Chr8 CDS 18891997 18892114 18891997 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18892196 18892410 18892196 ID=MsaT042228.1.exon2;Parent=MsaT042228.1
Chr8 CDS 18892196 18892410 18892196 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Chr8 exon 18892523 18892615 18892523 ID=MsaT042228.1.exon1;Parent=MsaT042228.1
Chr8 CDS 18892523 18892615 18892523 ID=cds.MsaT042228.1;Parent=MsaT042228.1
Gene Sequence

>MsaT042228.1

ATGCCATTTGGTTCTTTCTCTCAACCTCGTCTTGTTACTTCACCAGCTTTGGCTAAATCAATGTTCAACTCACCTGGATTATCACTTGCACTTCAAACAAATATTGATGGACAAGAAGATGTGAATGGATCAATGCATGGGAATTATGAGCAAAATGGTTTGAGAAGAAGTAGAGAAGAGGAACAAAGTAGATCTGGTAGTGATAACTTGGATGGTGTTTCTGGTGATGAACAAGATGCTGATGATAAACCACCTAGGAAGAAGAGATATCACCGACACACTCCTCAACAGATTCAAGAGCTTGAAAGTTTGTTTAAGGAGTGTCCTCACCCAGATGAGAAACAAAGATTGGAGCTTAGTAAAAGGCTTTGTTTGGAAACAAGACAAGTTAAGTTTTGGTTCCAAAATCGAAGAACACAAATGAAGACTCAATTGGAAAGGCATGAGAACAATTTGCTTAGGCAAGAGAATGATAAGCTTAGAGCTGAGAATATGTCTATAAGGGATGCAATGAGGAATCCTATTTGTTCGAATTGCGGTGGTCCAGCTATGATCGGCGAGATTTCACTCGAGGAACAACATCTTCGAATTGAGAATGCGAGGTTGAAGGACGAGCTAGATAGAGTTTGTGCACTTGCTGGAAAGTTTCTAGGCCGTCCGATTTCTTCGTTACCAAATTCAAGTTTAGAGCTTGGTGTTGGAGGAAACAACAACAATGGTTTTAATGTGATGAATAATGTGCCTTCAACATTACCTGATTTTAGTTCAGGAATGTCTAATAACCCTTTAGCCATTGTGTCTCCTTCAAATAGACAAACATCTATGGTGACTACTGGTTTTGATAGATCGGTCGAAAGGTCTATGTTTCTTGAACTTGCTTTGGCTGCTATGGATGAGTTGGTTAAGATGGCACAAACTAATGACCCTCTTTGGATTAGGAGTATAGAAGGAGGAAGGGAAATTTTGAACCATGAGGAATATTTGAGGACATTCACTCCTTGCATTGGTTTGAAACCTAATGGTTTTGTTTCTGAGGCCTCAAGGGAAACTGGTACTGTCATCATCAATAGCTTGGCCCTCGTCGAGACTCTTATGGACTCGAATCGATGGATAGAGATGTTTCCTTGTATCATTGCAAGAACCTCCACAAATGAAGTTATATCTAATGGAATAAATGGAACAAGAAATGGTGCACTTCAACTTATGCAAGCTGAACTTCATGTGCTATCGCCTTTGGTTCCGGTTCGCGAGGTAAATTTCCTTAGGTTTTGCAAGCAGCATGCAGAAGGTGTTTGGGCTGTGGTTGACGTGTCTATTGATACCATCAGAGAAACTTCTGGTGCTACAAGTTTTGTGAACTGTAGGAAGCTTCCCTCTGGTTGTGTTGTGCAAGATATGCCAAATGGTTACTCCAAGGTGACATGGGTGGAACATACAGAATATGAGGAAAATCAAGTCCACCAGCTGTATAGACCTCTGTTGAGCTCAGGTATGGGGTTTGGTGCGTCTCGTTGGGTCGCTACCTTACAACGCCAGTGCGAGTGCCTTGCCATATTAATGTCCTCTGCCGCTCCCTCTCGAGACCACTCTGCAATAACAGCAGGAGGAAGGCGAAGCATGCTGAAGCTCGCCCAGCGAATGACAAACAACTTCTGTGCCGGGGTCTGTGCCTCAACGGTCCACAAATGGAACAAGCTTAACCCTGGTAATGTAGACGAAGATGTGAGGGTGATGACACGAAAGAGCGTCGATGATCCAGGCGAGCCACCAGGGATCGTGTTGAGCGCAGCCACGTCGGTGTGGCTCCCGGTTTCGCCTCAAAGACTGTTTGATTTTCTGCGTGACGAAAGGCTCCGTAGCGAATGGGATATACTCTCAAATGGTGGTCCTATGCAAGAGATGGCTCATATTGCTAAAGGACAAGATCATGGCAACTGTGTTTCTCTTCTCCGTGCCAGTGCAATGAATTCCAACCAGAGTAGCATGTTGATACTACAAGAAACATGTATAGACGAAGCGGGGTCGCTTGTTGTCTATGCGCCGGTCGATATTCCAGCTATGCATGTTGTCATGAACGGCGGCGATTCAGCTTACGTGGCATTACTACCATCCGGCTTCGCCATCGTCCCTGACGGTCCTGGGTCACGCGGGCTAGAGAATGGCGATGCAACCGCAAATGGAGGCGGCGAGGCGCGTGTTAGCGGGTCCCTTTTAACTGTGGCGTTTCAAATCCTTGTGAATAGTCTTCCTACAGCAAAACTCACCGTGGAATCAGTTGAAACAGTGAACAATCTCATTTCATGCACTGTTCAGAAAATCAAAGCAGCACTTCAATGTGAAAGCTGA
Protein sequence

>MsaT042228.1

MPFGSFSQPRLVTSPALAKSMFNSPGLSLALQTNIDGQEDVNGSMHGNYEQNGLRRSREEEQSRSGSDNLDGVSGDEQDADDKPPRKKRYHRHTPQQIQELESLFKECPHPDEKQRLELSKRLCLETRQVKFWFQNRRTQMKTQLERHENNLLRQENDKLRAENMSIRDAMRNPICSNCGGPAMIGEISLEEQHLRIENARLKDELDRVCALAGKFLGRPISSLPNSSLELGVGGNNNNGFNVMNNVPSTLPDFSSGMSNNPLAIVSPSNRQTSMVTTGFDRSVERSMFLELALAAMDELVKMAQTNDPLWIRSIEGGREILNHEEYLRTFTPCIGLKPNGFVSEASRETGTVIINSLALVETLMDSNRWIEMFPCIIARTSTNEVISNGINGTRNGALQLMQAELHVLSPLVPVREVNFLRFCKQHAEGVWAVVDVSIDTIRETSGATSFVNCRKLPSGCVVQDMPNGYSKVTWVEHTEYEENQVHQLYRPLLSSGMGFGASRWVATLQRQCECLAILMSSAAPSRDHSAITAGGRRSMLKLAQRMTNNFCAGVCASTVHKWNKLNPGNVDEDVRVMTRKSVDDPGEPPGIVLSAATSVWLPVSPQRLFDFLRDERLRSEWDILSNGGPMQEMAHIAKGQDHGNCVSLLRASAMNSNQSSMLILQETCIDEAGSLVVYAPVDIPAMHVVMNGGDSAYVALLPSGFAIVPDGPGSRGLENGDATANGGGEARVSGSLLTVAFQILVNSLPTAKLTVESVETVNNLISCTVQKIKAALQCES*