AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042258

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042258 MtrunA17_Chr7g0269491 32.231 121 73 3 5 121 53 168 2.14e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCATTTGGAAGTAATT+AGG 0.245444 8:+19488374 MsaT042258.1:CDS
TAATTAAGTAATTACTAAAA+AGG 0.247246 8:-19488445 None:intergenic
GGTCTTCTTTAGACCAATAA+AGG 0.247419 8:-19488300 None:intergenic
ACTACGATTGCGCCCTTTAT+TGG 0.263600 8:+19488287 MsaT042258.1:CDS
TTCCCAACTATATTTCTTTC+CGG 0.282733 8:+19488128 MsaT042258.1:CDS
TGCATACCATGCATGCATTC+TGG 0.324425 8:-19488265 None:intergenic
TTCCGCAAGTTGGACACTTA+TGG 0.354700 8:-19488327 None:intergenic
AAAGGAGGAAATTGCACTTC+AGG 0.371770 8:-19488427 None:intergenic
ACCATTGTCTCAGCACCATT+TGG 0.383855 8:+19488363 MsaT042258.1:CDS
GATCGGGTGGGTGTCGGAAT+CGG 0.432374 8:-19488182 None:intergenic
AGTGTCCAACTTGCGGAATC+CGG 0.438842 8:+19488332 MsaT042258.1:CDS
TAGTTGGGAATCAGCTTCTT+CGG 0.444538 8:-19488115 None:intergenic
CAAATCAGACGACGACCATT+TGG 0.449941 8:+19488091 MsaT042258.1:CDS
TGAAAGTGGGAAGATGCTTC+CGG 0.459913 8:-19488147 None:intergenic
CAAGTTGGACACTTATGGTA+AGG 0.475386 8:-19488322 None:intergenic
GTCTTCTTTAGACCAATAAA+GGG 0.478289 8:-19488299 None:intergenic
GAGACAATGGTCTTTCAAAC+CGG 0.481043 8:-19488351 None:intergenic
TAAGCCTAATTACTTCCAAA+TGG 0.483290 8:-19488378 None:intergenic
TGTTACAAGGAGTTCGTAGA+TGG 0.488118 8:+19488223 MsaT042258.1:CDS
ATCAGCTTCTTCGGACCAAA+TGG 0.489732 8:-19488106 None:intergenic
CTTCCGGAAAGAAATATAGT+TGG 0.493805 8:-19488131 None:intergenic
GGAAGAGGAGTATGTGAAAG+TGG 0.502649 8:-19488161 None:intergenic
GTGGGTGTCGGAATCGGAAG+AGG 0.507812 8:-19488176 None:intergenic
TTCCGGAAAGAAATATAGTT+GGG 0.510227 8:-19488130 None:intergenic
CACTTCAGGAAAGTTTCTGA+TGG 0.510769 8:-19488413 None:intergenic
CGGAGCAAGATCCACCGATC+GGG 0.512178 8:-19488198 None:intergenic
TTTATTAGCATCAACATCCA+TGG 0.517609 8:-19488068 None:intergenic
TCCAAATGGTGCTGAGACAA+TGG 0.521723 8:-19488364 None:intergenic
AGCAAGATCCACCGATCGGG+TGG 0.529965 8:-19488195 None:intergenic
GAAGAGGAGTATGTGAAAGT+GGG 0.545248 8:-19488160 None:intergenic
ATCTTGCTCCGTCTGTTACA+AGG 0.551982 8:+19488210 MsaT042258.1:CDS
TCCACCGATCGGGTGGGTGT+CGG 0.559357 8:-19488188 None:intergenic
TCAAACCGGATTCCGCAAGT+TGG 0.559868 8:-19488337 None:intergenic
TACCATAAGTGTCCAACTTG+CGG 0.576135 8:+19488325 MsaT042258.1:CDS
TCCGACACCCACCCGATCGG+TGG 0.579668 8:+19488187 MsaT042258.1:CDS
TACGAACTCCTTGTAACAGA+CGG 0.602518 8:-19488218 None:intergenic
AAGTTGGACACTTATGGTAA+GGG 0.605876 8:-19488321 None:intergenic
GATTCCGACACCCACCCGAT+CGG 0.620723 8:+19488184 MsaT042258.1:CDS
ACGGAGCAAGATCCACCGAT+CGG 0.626627 8:-19488199 None:intergenic
ATACTTCCAGAATGCATGCA+TGG 0.632773 8:+19488259 MsaT042258.1:CDS
GCAAGATCCACCGATCGGGT+GGG 0.657120 8:-19488194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 19488070 19488453 19488070 ID=MsaG042258
Chr8 mRNA 19488070 19488453 19488070 ID=MsaT042258.1;Parent=MsaG042258
Chr8 exon 19488070 19488453 19488070 ID=MsaT042258.1.exon1;Parent=MsaT042258.1
Chr8 CDS 19488070 19488453 19488070 ID=cds.MsaT042258.1;Parent=MsaT042258.1
Gene Sequence

>MsaT042258.1

ATGGATGTTGATGCTAATAAACAAATCAGACGACGACCATTTGGTCCGAAGAAGCTGATTCCCAACTATATTTCTTTCCGGAAGCATCTTCCCACTTTCACATACTCCTCTTCCGATTCCGACACCCACCCGATCGGTGGATCTTGCTCCGTCTGTTACAAGGAGTTCGTAGATGGTGACAAAGAGTGTATACTTCCAGAATGCATGCATGGTATGCACTACGATTGCGCCCTTTATTGGTCTAAAGAAGACCCTTACCATAAGTGTCCAACTTGCGGAATCCGGTTTGAAAGACCATTGTCTCAGCACCATTTGGAAGTAATTAGGCTTATGAAAGAAACAACCATCAGAAACTTTCCTGAAGTGCAATTTCCTCCTTTTTAG
Protein sequence

>MsaT042258.1

MDVDANKQIRRRPFGPKKLIPNYISFRKHLPTFTYSSSDSDTHPIGGSCSVCYKEFVDGDKECILPECMHGMHYDCALYWSKEDPYHKCPTCGIRFERPLSQHHLEVIRLMKETTIRNFPEVQFPPF*