AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042282

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042282 MtrunA17_Chr8g0350941 94.362 337 9 2 1 331 1 333 0.0 640
MsaG042282 MtrunA17_Chr1g0204001 46.885 305 119 12 48 329 44 328 7.33e-69 218
MsaG042282 MtrunA17_Chr7g0224581 75.610 82 20 0 70 151 46 127 1.32e-38 138
MsaG042282 MtrunA17_Chr5g0416581 79.221 77 16 0 70 146 63 139 1.51e-37 137
MsaG042282 MtrunA17_Chr4g0060601 83.562 73 12 0 70 142 93 165 9.79e-37 137
MsaG042282 MtrunA17_Chr2g0277361 78.947 76 16 0 71 146 127 202 3.61e-36 135
MsaG042282 MtrunA17_Chr1g0180911 87.500 64 8 0 73 136 48 111 1.19e-34 125
MsaG042282 MtrunA17_Chr1g0211941 68.293 82 26 0 65 146 24 105 4.00e-32 120
MsaG042282 MtrunA17_Chr1g0164881 77.612 67 15 0 70 136 28 94 1.44e-31 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042282 AT2G45680.1 52.667 300 85 11 70 330 71 352 2.64e-79 246
MsaG042282 AT5G51910.1 51.128 266 80 10 70 324 53 279 6.90e-68 214
MsaG042282 AT5G51910.2 51.128 266 80 10 70 324 53 279 6.90e-68 214
MsaG042282 AT1G69690.1 83.562 73 12 0 70 142 48 120 1.99e-37 136
MsaG042282 AT3G47620.1 69.149 94 28 1 49 142 93 185 3.87e-36 136
MsaG042282 AT3G27010.2 87.879 66 8 0 70 135 74 139 1.80e-35 131
MsaG042282 AT3G27010.1 87.879 66 8 0 70 135 74 139 1.80e-35 131
MsaG042282 AT1G72010.1 78.082 73 16 0 74 146 63 135 1.36e-34 130
MsaG042282 AT1G58100.1 84.848 66 10 0 71 136 57 122 5.21e-34 129
MsaG042282 AT1G35560.1 75.342 73 18 0 74 146 55 127 1.05e-31 122
MsaG042282 AT5G23280.1 75.362 69 17 0 68 136 33 101 4.65e-31 117
MsaG042282 AT5G08330.1 77.612 67 15 0 70 136 27 93 5.16e-31 117
MsaG042282 AT5G41030.1 67.164 67 22 0 69 135 63 129 1.01e-27 108
MsaG042282 AT2G37000.1 78.333 60 13 0 74 133 43 102 1.41e-26 104
MsaG042282 AT3G45150.1 59.375 64 26 0 69 132 12 75 2.33e-21 89.7
MsaG042282 AT1G58100.2 86.111 36 5 0 71 106 57 92 1.52e-14 74.3

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGGCCTGGGTTTGGGCT+TGG 0.109243 8:-19759126 None:intergenic
CTTGAGCTTGGGCCTGGGTT+TGG 0.124774 8:-19759132 None:intergenic
TTGAGCTTGGGCCTGGGTTT+GGG 0.152560 8:-19759131 None:intergenic
ACCGATACATCTTCTATTTC+CGG 0.180539 8:-19759022 None:intergenic
TTGGGCCTGGGTTTGGGCTT+GGG 0.181827 8:-19759125 None:intergenic
GTTGGGCTTGAGCTTGGGCC+TGG 0.187452 8:-19759138 None:intergenic
TTCTATTTCCGGTTCCTCTT+TGG 0.188199 8:-19759011 None:intergenic
TGTTGGTTTGGTTCTTGATT+AGG 0.203445 8:-19759430 None:intergenic
GTTGGTTTGGTTCTTGATTA+GGG 0.229302 8:-19759429 None:intergenic
TTGTGGGTTGTTGAGGAATT+AGG 0.287595 8:-19759600 None:intergenic
TCTATTTCCGGTTCCTCTTT+GGG 0.291488 8:-19759010 None:intergenic
GAAGAAACAAAACCGGTTAT+TGG 0.308809 8:-19759742 None:intergenic
CGGACTTATGTCCGAGTTCT+CGG 0.317153 8:-19759270 None:intergenic
GGCGGGGACTGTGCCGGTTC+CGG 0.330630 8:-19759350 None:intergenic
GCTTGTTGAACTTGAATAAC+TGG 0.332426 8:-19759775 None:intergenic
GTTGTTGAGGAATTAGGTTT+AGG 0.343171 8:-19759594 None:intergenic
TTGGGCTTGAGCTTGGGCCT+GGG 0.356283 8:-19759137 None:intergenic
GACTGTGCCGGTTCCGGTGG+CGG 0.366048 8:-19759344 None:intergenic
ATCATTGCCGCCACCGGAAC+CGG 0.373706 8:+19759337 MsaT042282.1:CDS
TGTTTGTTGGGCTTGAGCTT+GGG 0.377692 8:-19759143 None:intergenic
CAGGTGATTCATCGGATTGT+TGG 0.389458 8:-19759447 None:intergenic
GATTCATCGGATTGTTGGTT+TGG 0.389852 8:-19759442 None:intergenic
CACAAAACATGGTTCCTGTA+TGG 0.395588 8:+19759632 MsaT042282.1:CDS
GTTTGAGGTTGTTATTAAAC+CGG 0.413851 8:-19759542 None:intergenic
TTGGGCTTGGGCTTGAACCA+AGG 0.418040 8:-19759113 None:intergenic
TGTGTTTGTGTTTATGTTTG+AGG 0.421063 8:-19759557 None:intergenic
AGAAACATTGATCTCACTCT+TGG 0.422119 8:+19758983 MsaT042282.1:CDS
CCGGTGGCGGCAATGATAGC+TGG 0.423227 8:-19759331 None:intergenic
TAGCTGGCTCCGCGTGTTCG+AGG 0.423931 8:-19759315 None:intergenic
TTTGGTACAACAGCGTTTGA+TGG 0.425689 8:-19759661 None:intergenic
CAAGGCTTTGTTGACTTGCA+TGG 0.428361 8:-19759095 None:intergenic
TTGTTTGTTGGGCTTGAGCT+TGG 0.430146 8:-19759144 None:intergenic
GTACTTGCTGCAACTGTGCT+TGG 0.438849 8:-19759853 None:intergenic
CTGCACATGTGGCAGGCATT+CGG 0.442371 8:-19759228 None:intergenic
TTGACTGACATGGCGATGGC+GGG 0.451232 8:-19759367 None:intergenic
TGGCAGGCATTCGGATCCTT+CGG 0.454844 8:-19759219 None:intergenic
TGGAGAGCCCAAAGAGGAAC+CGG 0.460137 8:+19759003 MsaT042282.1:CDS
CCAGCTATCATTGCCGCCAC+CGG 0.478903 8:+19759331 MsaT042282.1:CDS
ACCACCGTTTCTGTTTCAAC+CGG 0.480270 8:+19759523 MsaT042282.1:CDS
GATGACCATGCACGTGCCAC+CGG 0.487925 8:+19759060 MsaT042282.1:CDS
ACAAAACATGGTTCCTGTAT+GGG 0.488455 8:+19759633 MsaT042282.1:CDS
TCTACGTAAGCACTGTTTGA+AGG 0.493291 8:-19759490 None:intergenic
ACATTGGCTAGACCAATAAC+CGG 0.496766 8:+19759730 MsaT042282.1:CDS
ATGACCATGCACGTGCCACC+GGG 0.497135 8:+19759061 MsaT042282.1:CDS
GGACTTATGTCCGAGTTCTC+GGG 0.506633 8:-19759269 None:intergenic
ATCCGAGCTGCACATGTGGC+AGG 0.507450 8:-19759235 None:intergenic
GGCGATGGCGGGGACTGTGC+CGG 0.508002 8:-19759356 None:intergenic
GCCATCACTGTAGCTCTAGC+TGG 0.509355 8:-19759829 None:intergenic
TTGTTGACTTGCATGGACAT+AGG 0.520580 8:-19759088 None:intergenic
AACCATCGAAGAAACAAAAC+CGG 0.524083 8:-19759749 None:intergenic
AACTGAATTGTGGGTTGTTG+AGG 0.526600 8:-19759607 None:intergenic
TATCCCCGGTGGCACGTGCA+TGG 0.533191 8:-19759065 None:intergenic
TTTCAGCTGACCCGAGAACT+CGG 0.539737 8:+19759259 MsaT042282.1:CDS
TCAAACGCTGTTGTACCAAA+CGG 0.547610 8:+19759664 MsaT042282.1:CDS
GGTGGCACGTGCATGGTCAT+CGG 0.547673 8:-19759058 None:intergenic
CCAGCGAATTGTTTCGCCAT+CGG 0.548505 8:-19759290 None:intergenic
GGGGACTGTGCCGGTTCCGG+TGG 0.549398 8:-19759347 None:intergenic
CACACAAAAGTCGAGGGTCG+AGG 0.552285 8:+19759196 MsaT042282.1:CDS
ACCGGAAATAGAAGATGTAT+CGG 0.563512 8:+19759021 MsaT042282.1:CDS
TAAACCGGTTGAAACAGAAA+CGG 0.564370 8:-19759527 None:intergenic
TTTGATGGTATAGCCCATAC+AGG 0.566203 8:-19759646 None:intergenic
AAGTCGAGGGTCGAGGCCGA+AGG 0.575723 8:+19759203 MsaT042282.1:CDS
CATGCAAGTCAACAAAGCCT+TGG 0.580583 8:+19759096 MsaT042282.1:CDS
TGCATGGACATAGGTATCCC+CGG 0.581741 8:-19759079 None:intergenic
TGACTGACATGGCGATGGCG+GGG 0.586914 8:-19759366 None:intergenic
AATTCAGTTACCACAAAACA+TGG 0.589831 8:+19759621 MsaT042282.1:CDS
TGCCTGCCACATGTGCAGCT+CGG 0.589961 8:+19759233 MsaT042282.1:CDS
AAAGATCCGAGCTGCACATG+TGG 0.591484 8:-19759239 None:intergenic
GTTGACTGACATGGCGATGG+CGG 0.598518 8:-19759368 None:intergenic
GAGTGTGCCGTTGACTGACA+TGG 0.602740 8:-19759377 None:intergenic
CCGATGGCGAAACAATTCGC+TGG 0.602854 8:+19759290 MsaT042282.1:CDS
CACTCTTGGAGAGCCCAAAG+AGG 0.605171 8:+19758997 MsaT042282.1:CDS
AAGAGCTTCAGTTATTATCA+CGG 0.616423 8:+19759920 MsaT042282.1:CDS
GCCGTTGACTGACATGGCGA+TGG 0.617587 8:-19759371 None:intergenic
TCGCTGGCTCCTCGAACACG+CGG 0.627039 8:+19759306 MsaT042282.1:CDS
TCAAGCCCAAGCCCAAACCC+AGG 0.628129 8:+19759120 MsaT042282.1:CDS
GCCATCGCCATGTCAGTCAA+CGG 0.630285 8:+19759370 MsaT042282.1:CDS
AGACCGTCACACAAAAGTCG+AGG 0.630312 8:+19759189 MsaT042282.1:CDS
TCCAGCTAGAGCTACAGTGA+TGG 0.641074 8:+19759828 MsaT042282.1:CDS
GACCGTCACACAAAAGTCGA+GGG 0.645442 8:+19759190 MsaT042282.1:CDS
GAACTCGGACATAAGTCCGA+TGG 0.654955 8:+19759274 MsaT042282.1:CDS
ACCGGTTGAAACAGAAACGG+TGG 0.684445 8:-19759524 None:intergenic
TGACCATGCACGTGCCACCG+GGG 0.730870 8:+19759062 MsaT042282.1:CDS
ATGGACATAGGTATCCCCGG+TGG 0.809498 8:-19759076 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 19758965 19759960 19758965 ID=MsaG042282
Chr8 mRNA 19758965 19759960 19758965 ID=MsaT042282.1;Parent=MsaG042282
Chr8 exon 19758965 19759960 19758965 ID=MsaT042282.1.exon1;Parent=MsaT042282.1
Chr8 CDS 19758965 19759960 19758965 ID=cds.MsaT042282.1;Parent=MsaT042282.1
Gene Sequence

>MsaT042282.1

ATGGCTGAAGAAAATTCAAGAAACATTGATCTCACTCTTGGAGAGCCCAAAGAGGAACCGGAAATAGAAGATGTATCGGTAATAAAAGTTATGCCGATGACCATGCACGTGCCACCGGGGATACCTATGTCCATGCAAGTCAACAAAGCCTTGGTTCAAGCCCAAGCCCAAACCCAGGCCCAAGCTCAAGCCCAACAAACAATTCAAAAGAGATCATCCACAAAAGACCGTCACACAAAAGTCGAGGGTCGAGGCCGAAGGATCCGAATGCCTGCCACATGTGCAGCTCGGATCTTTCAGCTGACCCGAGAACTCGGACATAAGTCCGATGGCGAAACAATTCGCTGGCTCCTCGAACACGCGGAGCCAGCTATCATTGCCGCCACCGGAACCGGCACAGTCCCCGCCATCGCCATGTCAGTCAACGGCACACTCAAAATCCCAACCACCTCACCAACCAAAAACCCTAATCAAGAACCAAACCAACAATCCGATGAATCACCTGAGAAAAAGAAACGTAAACGACCTTCAAACAGTGCTTACGTAGATATAAACGACACCACCGTTTCTGTTTCAACCGGTTTAATAACAACCTCAAACATAAACACAAACACAAACACAAACACAAACCTAAACCTAATTCCTCAACAACCCACAATTCAGTTACCACAAAACATGGTTCCTGTATGGGCTATACCATCAAACGCTGTTGTACCAAACGGAAATACTTTTTTCGTTGTTCCAACAAATCAACCACAGTTTTTTACATTGGCTAGACCAATAACCGGTTTTGTTTCTTCGATGGTTACACCAGTTATTCAAGTTCAACAAGCTTCATGTTCATTATCTCCAGCAACAAAATCTCCAGCTAGAGCTACAGTGATGGCTCCAAGCACAGTTGCAGCAAGTACAACTCAGATGTTGAGAGATTTTTCATTGGAGATTTATGATAGACAAGAGCTTCAGTTATTATCACGGTCTTCTTCAAAGCATTGA
Protein sequence

>MsaT042282.1

MAEENSRNIDLTLGEPKEEPEIEDVSVIKVMPMTMHVPPGIPMSMQVNKALVQAQAQTQAQAQAQQTIQKRSSTKDRHTKVEGRGRRIRMPATCAARIFQLTRELGHKSDGETIRWLLEHAEPAIIAATGTGTVPAIAMSVNGTLKIPTTSPTKNPNQEPNQQSDESPEKKKRKRPSNSAYVDINDTTVSVSTGLITTSNINTNTNTNTNLNLIPQQPTIQLPQNMVPVWAIPSNAVVPNGNTFFVVPTNQPQFFTLARPITGFVSSMVTPVIQVQQASCSLSPATKSPARATVMAPSTVAASTTQMLRDFSLEIYDRQELQLLSRSSSKH*