AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042414

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042414 MtrunA17_Chr8g0352011 96.386 249 7 2 1 248 93 340 0.0 501
MsaG042414 MtrunA17_Chr1g0173841 72.541 244 56 3 15 247 145 388 1.07e-130 374
MsaG042414 MtrunA17_Chr7g0258011 71.849 238 64 1 9 243 108 345 2.75e-125 359
MsaG042414 MtrunA17_Chr2g0328921 72.996 237 55 4 12 246 83 312 4.29e-122 350
MsaG042414 MtrunA17_Chr5g0422571 62.393 234 66 4 14 243 57 272 1.35e-104 305
MsaG042414 MtrunA17_Chr2g0321291 62.128 235 82 4 4 235 65 295 3.51e-103 301
MsaG042414 MtrunA17_Chr4g0026521 63.274 226 69 3 21 243 76 290 2.05e-101 296
MsaG042414 MtrunA17_Chr4g0053341 68.421 209 45 2 17 225 38 225 1.84e-98 287
MsaG042414 MtrunA17_Chr1g0198651 74.233 163 39 1 71 230 99 261 2.61e-94 278
MsaG042414 MtrunA17_Chr1g0200421 35.610 205 114 6 29 218 186 387 1.24e-26 107
MsaG042414 MtrunA17_Chr2g0320341 37.584 149 85 4 81 222 238 385 5.13e-23 96.7
MsaG042414 MtrunA17_Chr1g0191991 35.106 188 96 8 60 239 59 228 3.78e-21 92.0
MsaG042414 MtrunA17_Chr1g0163001 30.928 194 105 7 45 218 11 195 7.04e-21 91.3
MsaG042414 MtrunA17_Chr1g0151351 36.620 142 79 5 79 218 62 194 8.60e-21 90.5
MsaG042414 MtrunA17_Chr8g0382121 37.313 134 80 3 89 218 127 260 1.31e-20 88.6
MsaG042414 MtrunA17_Chr1g0210821 36.301 146 83 5 74 218 60 196 1.60e-20 90.1
MsaG042414 MtrunA17_Chr4g0029521 32.124 193 117 6 28 218 11 191 2.26e-20 89.7
MsaG042414 MtrunA17_Chr1g0162971 38.129 139 76 5 81 218 64 193 2.31e-20 89.4
MsaG042414 MtrunA17_Chr7g0268961 33.929 168 85 8 75 234 33 182 2.76e-20 89.4
MsaG042414 MtrunA17_Chr1g0199101 36.301 146 83 5 74 218 63 199 3.00e-20 89.4
MsaG042414 MtrunA17_Chr1g0185121 32.512 203 114 7 26 218 4 193 8.25e-20 87.8
MsaG042414 MtrunA17_Chr2g0326381 35.616 146 85 4 74 218 48 185 8.59e-20 87.4
MsaG042414 MtrunA17_Chr8g0342421 35.252 139 80 4 81 218 85 214 1.21e-19 87.4
MsaG042414 MtrunA17_Chr2g0330171 34.247 146 86 4 74 218 62 198 2.16e-19 86.7
MsaG042414 MtrunA17_Chr7g0263941 36.735 147 81 6 74 218 50 186 2.92e-19 85.9
MsaG042414 MtrunA17_Chr8g0373541 33.333 189 100 7 54 234 23 193 3.53e-19 86.3
MsaG042414 MtrunA17_Chr1g0181381 33.562 146 88 4 74 218 54 191 7.45e-19 85.1
MsaG042414 MtrunA17_Chr2g0324991 35.915 142 81 5 74 213 8 141 1.93e-18 79.3
MsaG042414 MtrunA17_Chr3g0123941 38.158 152 82 7 89 236 139 282 1.95e-18 83.2
MsaG042414 MtrunA17_Chr4g0040571 31.818 176 102 5 74 248 55 213 7.04e-18 82.4
MsaG042414 MtrunA17_Chr3g0141431 33.333 138 82 4 79 214 43 172 5.95e-16 76.6
MsaG042414 MtrunA17_Chr6g0479921 31.034 145 90 5 79 221 50 186 2.38e-13 68.9
MsaG042414 MtrunA17_Chr8g0375521 32.394 142 85 5 79 217 48 181 3.32e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042414 AT3G57670.1 68.000 250 53 1 19 241 122 371 2.02e-120 348
MsaG042414 AT3G20880.1 66.245 237 65 4 18 241 175 409 9.07e-110 322
MsaG042414 AT1G51220.1 64.344 244 68 4 14 241 96 336 7.67e-108 315
MsaG042414 AT1G13290.1 62.054 224 61 5 7 227 41 243 2.46e-96 284
MsaG042414 AT1G34790.1 62.332 223 78 2 19 241 83 299 4.20e-96 284
MsaG042414 AT1G08290.1 57.028 249 87 5 7 235 84 332 7.69e-96 284
MsaG042414 AT1G34370.2 32.407 216 128 5 19 218 169 382 4.98e-28 111
MsaG042414 AT1G34370.1 32.407 216 128 5 19 218 169 382 4.98e-28 111
MsaG042414 AT1G34370.3 39.456 147 78 4 81 218 28 172 7.09e-27 105
MsaG042414 AT5G22890.1 38.621 145 82 3 81 218 211 355 8.34e-24 98.6
MsaG042414 AT1G55110.3 38.028 142 77 6 79 218 84 216 5.69e-21 91.7
MsaG042414 AT1G55110.1 38.028 142 77 6 79 218 84 216 5.69e-21 91.7
MsaG042414 AT1G55110.2 38.028 142 77 6 79 218 84 216 5.69e-21 91.7
MsaG042414 AT2G02080.1 36.879 141 79 4 79 218 75 206 1.69e-20 90.5
MsaG042414 AT2G02080.4 36.879 141 79 4 79 218 75 206 1.69e-20 90.5
MsaG042414 AT2G02080.5 36.879 141 79 4 79 218 75 206 1.69e-20 90.5
MsaG042414 AT2G02080.3 36.879 141 79 4 79 218 75 206 1.69e-20 90.5
MsaG042414 AT1G14580.1 32.787 183 100 6 50 218 32 205 1.74e-20 90.1
MsaG042414 AT1G14580.2 32.787 183 100 6 50 218 32 205 1.74e-20 90.1
MsaG042414 AT2G02070.2 36.301 146 83 4 74 218 68 204 1.77e-20 90.5
MsaG042414 AT2G02070.1 36.301 146 83 4 74 218 68 204 1.77e-20 90.5
MsaG042414 AT1G14580.3 32.787 183 100 6 50 218 46 219 1.89e-20 90.1
MsaG042414 AT5G03150.1 33.333 165 93 5 80 243 75 223 5.56e-20 89.0
MsaG042414 AT2G02080.6 36.957 138 77 4 82 218 1 129 6.42e-20 88.6
MsaG042414 AT2G02080.2 36.957 138 77 4 82 218 1 129 6.42e-20 88.6
MsaG042414 AT5G66730.1 35.915 142 79 5 79 218 53 184 7.29e-19 85.5
MsaG042414 AT5G44160.2 33.133 166 93 5 81 245 21 169 1.18e-18 84.7
MsaG042414 AT5G44160.1 32.934 167 94 5 80 245 59 208 1.21e-18 85.1
MsaG042414 AT1G03840.2 34.247 146 86 5 74 218 55 191 2.00e-18 84.3
MsaG042414 AT1G03840.1 34.247 146 86 5 74 218 57 193 2.10e-18 84.3
MsaG042414 AT3G45260.2 36.691 139 79 4 81 218 62 192 2.59e-18 84.0
MsaG042414 AT3G45260.1 36.691 139 79 4 81 218 62 192 2.59e-18 84.0
MsaG042414 AT5G60470.1 35.294 136 78 4 81 214 58 185 6.16e-18 82.8
MsaG042414 AT5G60470.2 35.294 136 78 4 81 214 67 194 6.69e-18 82.8
MsaG042414 AT5G60470.3 35.294 136 78 4 81 214 63 190 6.81e-18 82.8
MsaG042414 AT4G02670.2 33.562 146 88 5 74 218 71 208 1.20e-17 81.6
MsaG042414 AT4G02670.1 33.562 146 88 5 74 218 69 206 1.41e-17 81.6
MsaG042414 AT3G13810.4 34.014 147 86 5 74 218 86 223 1.53e-17 82.0
MsaG042414 AT3G13810.1 34.014 147 86 5 74 218 86 223 1.53e-17 82.0
MsaG042414 AT3G13810.5 34.014 147 86 5 74 218 81 218 1.66e-17 81.6
MsaG042414 AT3G13810.6 34.014 147 86 5 74 218 67 204 1.91e-17 81.6
MsaG042414 AT3G13810.2 34.014 147 86 5 74 218 87 224 1.91e-17 81.6
MsaG042414 AT3G13810.3 34.014 147 86 5 74 218 73 210 2.10e-17 81.3
MsaG042414 AT3G50700.1 33.544 158 85 6 79 234 55 194 2.60e-17 80.9
MsaG042414 AT1G25250.1 28.758 153 99 5 65 214 17 162 1.24e-13 70.1
MsaG042414 AT1G25250.3 30.935 139 86 5 79 214 54 185 1.41e-13 70.1
MsaG042414 AT1G68130.1 31.655 139 84 5 79 214 62 192 5.62e-13 68.2
MsaG042414 AT2G01940.1 30.935 139 85 5 79 214 65 195 1.54e-11 63.9

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACTGTTGCACTACAAAT+AGG 0.213717 8:-21521625 MsaT042414.1:CDS
CTTCCTAGTAATTCTTCTTC+TGG 0.261541 8:-21521601 MsaT042414.1:CDS
AGTAATTCTTCTTCTGGTCT+TGG 0.280359 8:-21521595 MsaT042414.1:CDS
GGTTATGGTCATGGTGCTTT+TGG 0.295781 8:-21519319 MsaT042414.1:CDS
CATATTAAAGCCTTTGGTTA+TGG 0.310320 8:-21519334 MsaT042414.1:CDS
AGAAGAATTGTGGCAAAATA+TGG 0.329283 8:-21519405 MsaT042414.1:CDS
AAGGGGCCAGATTCTCTAAA+AGG 0.343680 8:-21519631 MsaT042414.1:CDS
AAAGACCATATTAAAGCCTT+TGG 0.344662 8:-21519340 MsaT042414.1:CDS
CTTCTTCTGGTCTTGGATCA+AGG 0.347469 8:-21521588 MsaT042414.1:CDS
TTCTGAACATCAACAACCTT+TGG 0.351430 8:-21521512 MsaT042414.1:CDS
TTACTGTTGCACTACAAATA+GGG 0.383274 8:-21521624 MsaT042414.1:CDS
CATAACCAAAGGCTTTAATA+TGG 0.384327 8:+21519335 None:intergenic
CAACAGATACAACAATCTTC+AGG 0.407884 8:-21521392 MsaT042414.1:intron
CTCATCAAATAGCATAGAAA+TGG 0.420829 8:-21521560 MsaT042414.1:CDS
CATTTGCAGTGAAAGGTGAT+TGG 0.425078 8:-21519438 MsaT042414.1:CDS
ACACCTTCTCAAATTCTCAT+TGG 0.440374 8:-21521454 MsaT042414.1:CDS
CCATGCTATTGTTGTGCACC+AGG 0.454587 8:-21519580 MsaT042414.1:CDS
TGAACAACAAGGGTCAGTAT+TGG 0.475729 8:-21521483 MsaT042414.1:CDS
AAAGCCTTTGGTTATGGTCA+TGG 0.479825 8:-21519328 MsaT042414.1:CDS
TTCTGGTCTTGGATCAAGGA+TGG 0.485959 8:-21521584 MsaT042414.1:CDS
TGAGAGGCTTTGCTCTAGGA+TGG 0.497344 8:+21519539 None:intergenic
CAGATGCATATGTGGGGACA+TGG 0.509465 8:-21519667 MsaT042414.1:CDS
TCTTTGAGAGGCTTTGCTCT+AGG 0.513133 8:+21519535 None:intergenic
ATGAGAATTTGAGAAGGTGT+TGG 0.518525 8:+21521457 None:intergenic
CCTTATATGTGTAGAAAATG+TGG 0.531273 8:-21519466 MsaT042414.1:CDS
AGACCAGAAGAAGAATTACT+AGG 0.533564 8:+21521598 None:intergenic
CATGGATCTCAATACAGAAA+GGG 0.536031 8:-21519649 MsaT042414.1:CDS
TTGCAAGAAGAAGATGAAGT+AGG 0.553002 8:-21519286 MsaT042414.1:CDS
GACAAGAGAATGGAAAGGCA+TGG 0.561442 8:-21521688 None:intergenic
TGCAAGAAGAAGATGAAGTA+GGG 0.562185 8:-21519285 MsaT042414.1:CDS
ACACATTATAAGAGAAAGCA+TGG 0.564164 8:-21519496 MsaT042414.1:CDS
TTGTTGTTCAACTTATCCAA+AGG 0.565952 8:+21521496 None:intergenic
GGTAGCCTTAGCATTGCTGT+TGG 0.572264 8:+21519601 None:intergenic
CACAACCAACAGCAATGCTA+AGG 0.579227 8:-21519606 MsaT042414.1:CDS
AGAGTTCTAAAATCTTTGAG+AGG 0.584007 8:+21519523 None:intergenic
ACATGGATCTCAATACAGAA+AGG 0.588548 8:-21519650 MsaT042414.1:CDS
AAAATATGGTATTGTTTGTG+TGG 0.602783 8:-21519391 MsaT042414.1:CDS
GGGTCAGAAATTGAACATGA+TGG 0.603687 8:-21519265 MsaT042414.1:CDS
TAGAAGACAAGAGAATGGAA+AGG 0.616360 8:-21521693 None:intergenic
GGCAAATCATTTGCAGTGAA+AGG 0.616551 8:-21519445 MsaT042414.1:CDS
ATGGATCTCAATACAGAAAG+GGG 0.624745 8:-21519648 MsaT042414.1:CDS
AGAACACATGAGAAGAATTG+TGG 0.625082 8:-21519415 MsaT042414.1:CDS
TCAATGTTATGCTTGCAACC+TGG 0.646006 8:+21519562 None:intergenic
TCAGAAATTGAACATGATGG+TGG 0.646397 8:-21519262 MsaT042414.1:CDS
TTGGATAAGTTGAACAACAA+GGG 0.662388 8:-21521493 MsaT042414.1:CDS
TTTGGATAAGTTGAACAACA+AGG 0.664103 8:-21521494 MsaT042414.1:CDS
AGCACCATGACCATAACCAA+AGG 0.668968 8:+21519324 None:intergenic
ACAGGACAAGAGAATTGCGT+AGG 0.670453 8:+21521430 None:intergenic
CCTGGTGCACAACAATAGCA+TGG 0.670992 8:+21519580 None:intergenic
GGACCAATGAGAATTTGAGA+AGG 0.690488 8:+21521451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 21519249 21521701 21519249 ID=MsaG042414
Chr8 mRNA 21519249 21521701 21519249 ID=MsaT042414.1;Parent=MsaG042414
Chr8 exon 21519249 21519686 21519249 ID=MsaT042414.1.exon2;Parent=MsaT042414.1
Chr8 CDS 21519249 21519686 21519249 ID=cds.MsaT042414.1;Parent=MsaT042414.1
Chr8 exon 21521393 21521701 21521393 ID=MsaT042414.1.exon1;Parent=MsaT042414.1
Chr8 CDS 21521393 21521701 21521393 ID=cds.MsaT042414.1;Parent=MsaT042414.1
Gene Sequence

>MsaT042414.1

ATGGAAAGGCATGGATATAATCATGATGATGATGATGATCTTGATGATGAAAATGTTACTGTTGCACTACAAATAGGGCTTCCTAGTAATTCTTCTTCTGGTCTTGGATCAAGGATGGTCTCATCAAATAGCATAGAAATGGCTGAAAAAGAGGAAGTTAATATGATTTCTGAACATCAACAACCTTTGGATAAGTTGAACAACAAGGGTCAGTATTGGATTCCAACACCTTCTCAAATTCTCATTGGTCCTACGCAATTCTCTTGTCCTGTGTGCTCTAAAACTTTCAACAGATACAACAATCTTCAGATGCATATGTGGGGACATGGATCTCAATACAGAAAGGGGCCAGATTCTCTAAAAGGATCACAACCAACAGCAATGCTAAGGCTACCATGCTATTGTTGTGCACCAGGTTGCAAGCATAACATTGACCATCCTAGAGCAAAGCCTCTCAAAGATTTTAGAACTCTTCAAACACATTATAAGAGAAAGCATGGTATAAAACCTTATATGTGTAGAAAATGTGGCAAATCATTTGCAGTGAAAGGTGATTGGAGAACACATGAGAAGAATTGTGGCAAAATATGGTATTGTTTGTGTGGCTCAGATTTTAAACATAAAAGGTCTCTTAAAGACCATATTAAAGCCTTTGGTTATGGTCATGGTGCTTTTGGTATTGATTGCTTGCAAGAAGAAGATGAAGTAGGGTCAGAAATTGAACATGATGGTGGAAGTTCAATGTGA
Protein sequence

>MsaT042414.1

MERHGYNHDDDDDLDDENVTVALQIGLPSNSSSGLGSRMVSSNSIEMAEKEEVNMISEHQQPLDKLNNKGQYWIPTPSQILIGPTQFSCPVCSKTFNRYNNLQMHMWGHGSQYRKGPDSLKGSQPTAMLRLPCYCCAPGCKHNIDHPRAKPLKDFRTLQTHYKRKHGIKPYMCRKCGKSFAVKGDWRTHEKNCGKIWYCLCGSDFKHKRSLKDHIKAFGYGHGAFGIDCLQEEDEVGSEIEHDGGSSM*