AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042426

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042426 MtrunA17_Chr8g0352251 94.898 294 15 0 1 294 1 294 0.0 580
MsaG042426 MtrunA17_Chr3g0103391 45.933 209 91 5 21 209 20 226 3.09e-47 160
MsaG042426 MtrunA17_Chr2g0300261 49.669 151 67 4 114 259 33 179 3.77e-37 132
MsaG042426 MtrunA17_Chr2g0292411 71.111 90 26 0 127 216 86 175 1.58e-36 129
MsaG042426 MtrunA17_Chr8g0343451 45.614 171 71 7 115 283 128 278 3.18e-33 124
MsaG042426 MtrunA17_Chr2g0329811 56.190 105 41 1 117 216 144 248 1.97e-31 119
MsaG042426 MtrunA17_Chr7g0267581 51.200 125 47 5 98 219 118 231 1.32e-30 117
MsaG042426 MtrunA17_Chr1g0177091 49.593 123 49 4 109 229 128 239 6.61e-29 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042426 AT5G12840.4 38.430 242 122 7 27 245 25 262 1.84e-39 139
MsaG042426 AT5G12840.2 38.430 242 122 7 27 245 25 262 1.84e-39 139
MsaG042426 AT5G12840.5 47.917 144 74 1 103 245 120 263 4.13e-39 138
MsaG042426 AT5G12840.3 47.917 144 74 1 103 245 120 263 4.13e-39 138
MsaG042426 AT5G12840.1 47.917 144 74 1 103 245 120 263 4.13e-39 138
MsaG042426 AT3G20910.1 47.619 147 75 1 98 242 108 254 6.48e-39 139
MsaG042426 AT1G72830.1 56.250 112 46 3 115 223 137 248 2.27e-32 122
MsaG042426 AT1G17590.3 52.239 134 48 6 86 216 114 234 3.08e-32 122
MsaG042426 AT1G17590.2 52.239 134 48 6 86 216 114 234 3.08e-32 122
MsaG042426 AT1G17590.4 52.239 134 48 6 86 216 114 234 3.08e-32 122
MsaG042426 AT1G17590.1 52.239 134 48 6 86 216 114 234 3.08e-32 122
MsaG042426 AT1G30500.2 50.758 132 57 3 114 237 59 190 4.25e-31 115
MsaG042426 AT1G30500.1 62.245 98 30 2 147 237 89 186 6.84e-31 114
MsaG042426 AT1G72830.2 55.752 113 46 4 115 223 137 249 1.21e-30 118
MsaG042426 AT3G14020.2 59.794 97 36 2 129 224 145 239 1.62e-30 117
MsaG042426 AT3G14020.1 59.794 97 36 2 129 224 145 239 1.62e-30 117
MsaG042426 AT2G34720.1 46.154 143 70 4 102 239 45 185 1.12e-29 112
MsaG042426 AT3G05690.1 35.102 245 118 9 31 255 18 241 1.58e-29 114
MsaG042426 AT3G05690.2 32.959 267 138 9 9 255 33 278 1.77e-29 114
MsaG042426 AT2G34720.2 49.231 130 60 3 114 239 23 150 1.98e-29 110
MsaG042426 AT2G34720.3 49.231 130 60 3 114 239 23 150 1.98e-29 110
MsaG042426 AT5G06510.1 35.961 203 86 7 30 213 13 190 2.02e-29 113
MsaG042426 AT5G06510.5 35.961 203 86 7 30 213 13 190 2.02e-29 113
MsaG042426 AT5G06510.2 35.961 203 86 7 30 213 13 190 2.02e-29 113
MsaG042426 AT5G06510.6 35.961 203 86 7 30 213 13 190 2.02e-29 113
MsaG042426 AT1G54160.1 69.333 75 22 1 143 216 166 240 4.82e-29 113
MsaG042426 AT5G06510.4 55.102 98 29 3 119 213 10 95 5.95e-28 106
MsaG042426 AT5G06510.7 55.102 98 29 3 119 213 10 95 5.95e-28 106
MsaG042426 AT5G06510.3 55.102 98 29 3 119 213 56 141 2.38e-27 106
MsaG042426 AT1G72830.3 54.217 83 38 0 141 223 141 223 1.95e-22 95.1

Find 70 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGTTCCGTCTGCGAATT+TGG 0.146875 8:-21817516 None:intergenic
ATTCTCCAATCATCAGTTTC+TGG 0.161621 8:-21816773 None:intergenic
CCGAATTGTGCATTTGAATA+AGG 0.229160 8:-21817634 None:intergenic
GGTCAAATCTTGAATGCTTA+TGG 0.231694 8:+21817655 MsaT042426.1:CDS
TCTAATGCTGCTCTAGAAAA+AGG 0.271832 8:+21819224 MsaT042426.1:CDS
CAGCCTTGGTGGCTTGAAAA+TGG 0.313297 8:+21816845 MsaT042426.1:CDS
AAACAGGAGGGTGACTTCTT+TGG 0.313426 8:+21819395 MsaT042426.1:CDS
ACAGGGCATGTTTGATGTTT+CGG 0.314790 8:-21817458 None:intergenic
AGGCTGATGATGAGAGTAAA+TGG 0.326121 8:-21816828 None:intergenic
CTGATCAGTTGAAGATGGTT+TGG 0.350260 8:-21816870 None:intergenic
TGTCTCACATTCAGAGCCTT+TGG 0.393769 8:+21816916 MsaT042426.1:CDS
GCATTCTAGCATGATGCATT+TGG 0.399038 8:-21818581 None:intergenic
TTGAGAAGGGCAAGGGGAAA+TGG 0.400323 8:+21819161 MsaT042426.1:CDS
GAAAGTTATTAAAGTTAGAA+AGG 0.407898 8:+21818707 MsaT042426.1:CDS
TGAGCCTTGATTCTCATTGT+TGG 0.412750 8:-21819273 None:intergenic
TGGTAAATGCGGTTGAGGAC+AGG 0.424267 8:-21817476 None:intergenic
TCATCAGTTTCTGGTTTCCC+TGG 0.428859 8:-21816764 None:intergenic
TTCACAGGCAAATGGAAAAC+AGG 0.431715 8:+21819379 MsaT042426.1:CDS
AAAAGCTGTGAAAGGATGCC+AGG 0.439397 8:+21816746 None:intergenic
CCTTATTCAAATGCACAATT+CGG 0.445518 8:+21817634 MsaT042426.1:CDS
ACTCTCATCATCAGCCTTGG+TGG 0.446091 8:+21816834 MsaT042426.1:CDS
TTTAGCTGATCAGTTGAAGA+TGG 0.474075 8:-21816875 None:intergenic
GTTCGAGCTTCTTTGTATTG+AGG 0.481503 8:-21819193 None:intergenic
GCAGCACATGCACAAAATGC+AGG 0.482353 8:+21819307 MsaT042426.1:CDS
CTATGTCATTCACAGGCAAA+TGG 0.486227 8:+21819371 MsaT042426.1:CDS
TTTACTCTCATCATCAGCCT+TGG 0.487165 8:+21816831 MsaT042426.1:CDS
GGAAACCAGAAACTGATGAT+TGG 0.490368 8:+21816768 MsaT042426.1:CDS
AACAATACTGGTGCAACATC+GGG 0.490937 8:+21819248 MsaT042426.1:CDS
GTCCATGGTAAATGCGGTTG+AGG 0.491108 8:-21817481 None:intergenic
TCTCAAAGCATGTTGGTGAC+GGG 0.492087 8:-21819144 None:intergenic
TTCTCAAAGCATGTTGGTGA+CGG 0.494518 8:-21819145 None:intergenic
TGACGGGATTCATGAAGATA+TGG 0.495855 8:-21819128 None:intergenic
AATTCGCAGACGGAACTTGT+TGG 0.504090 8:+21817519 MsaT042426.1:CDS
GAGAATAAAACATAGTGAGC+AGG 0.507446 8:+21816790 MsaT042426.1:CDS
CTCAGGTGGGGACGTTGCCA+AGG 0.514046 8:+21817431 MsaT042426.1:intron
TTCAACATCAGTTACCATGA+TGG 0.518987 8:+21819332 MsaT042426.1:CDS
GTCAATGCAAAGCAGTATCA+TGG 0.520607 8:+21818639 MsaT042426.1:CDS
TGGACCAAGGTGTTTGTCCA+TGG 0.523692 8:-21817496 None:intergenic
TTTGATGTTTCGGTGCTCCT+TGG 0.525070 8:-21817448 None:intergenic
CAACAATACTGGTGCAACAT+CGG 0.530920 8:+21819247 MsaT042426.1:CDS
TGCTTTGCATTGACATACAC+AGG 0.531370 8:-21818630 None:intergenic
GGTAAATGCGGTTGAGGACA+GGG 0.539516 8:-21817475 None:intergenic
TTGCCCTTCTCAAAGCATGT+TGG 0.541677 8:-21819151 None:intergenic
CTGTAAAGTCATTTCCATCA+TGG 0.545256 8:-21819346 None:intergenic
TACAGCACTATGTCATTCAC+AGG 0.550828 8:+21819364 MsaT042426.1:CDS
TGCTTATGGACAACAAGCTA+TGG 0.551820 8:+21817669 MsaT042426.1:CDS
TTTGCCGCTTAAAATGGAAG+AGG 0.565386 8:+21818605 MsaT042426.1:CDS
ACATGCTTTGAGAAGGGCAA+GGG 0.570857 8:+21819154 MsaT042426.1:CDS
GTCACCAACATGCTTTGAGA+AGG 0.572667 8:+21819147 MsaT042426.1:CDS
ACGAAGACAATCGCGTGCAA+AGG 0.578373 8:+21818671 MsaT042426.1:CDS
GGTACCAACAATGAGAATCA+AGG 0.578464 8:+21819269 MsaT042426.1:CDS
TTTACCATGGACAAACACCT+TGG 0.581517 8:+21817492 MsaT042426.1:CDS
CATGCTTTGAGAAGGGCAAG+GGG 0.591440 8:+21819155 MsaT042426.1:CDS
GATGCATTTGGTATAGCTGA+GGG 0.592164 8:-21818569 None:intergenic
TTATCAGTTAGAGAAAGTGA+AGG 0.593911 8:-21816944 None:intergenic
TGATGCATTTGGTATAGCTG+AGG 0.596710 8:-21818570 None:intergenic
GAAAGTGAAGGAGAAACCAA+AGG 0.608239 8:-21816932 None:intergenic
TCACCAACATGCTTTGAGAA+GGG 0.609839 8:+21819148 MsaT042426.1:CDS
GTCCTCAACCGCATTTACCA+TGG 0.610316 8:+21817479 MsaT042426.1:CDS
CTTTCTCTAACTGATAACTC+AGG 0.610628 8:+21816950 MsaT042426.1:CDS
TGATGAGAGTAAATGGAAGA+TGG 0.612845 8:-21816821 None:intergenic
CAGCACATGCACAAAATGCA+GGG 0.616147 8:+21819308 MsaT042426.1:CDS
AAAGCTGTGAAAGGATGCCA+GGG 0.629084 8:+21816747 None:intergenic
AACATGCTTTGAGAAGGGCA+AGG 0.629479 8:+21819153 MsaT042426.1:CDS
ACAGGCAAATGGAAAACAGG+AGG 0.631076 8:+21819382 MsaT042426.1:CDS
CAGGCAAATGGAAAACAGGA+GGG 0.651426 8:+21819383 MsaT042426.1:CDS
AGAAGGGCAAGGGGAAATGG+CGG 0.651466 8:+21819164 MsaT042426.1:CDS
CCTTGGTCCAAATTCGCAGA+CGG 0.654666 8:+21817509 MsaT042426.1:CDS
CCGTCTGCGAATTTGGACCA+AGG 0.662623 8:-21817509 None:intergenic
GTGTTTGTCCATGGTAAATG+CGG 0.682262 8:-21817487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 21816761 21819451 21816761 ID=MsaG042426
Chr8 mRNA 21816761 21819451 21816761 ID=MsaT042426.1;Parent=MsaG042426
Chr8 exon 21816761 21816971 21816761 ID=MsaT042426.1.exon1;Parent=MsaT042426.1
Chr8 CDS 21816761 21816971 21816761 ID=cds.MsaT042426.1;Parent=MsaT042426.1
Chr8 exon 21817436 21817551 21817436 ID=MsaT042426.1.exon2;Parent=MsaT042426.1
Chr8 CDS 21817436 21817551 21817436 ID=cds.MsaT042426.1;Parent=MsaT042426.1
Chr8 exon 21817622 21817690 21817622 ID=MsaT042426.1.exon3;Parent=MsaT042426.1
Chr8 CDS 21817622 21817690 21817622 ID=cds.MsaT042426.1;Parent=MsaT042426.1
Chr8 exon 21818564 21818728 21818564 ID=MsaT042426.1.exon4;Parent=MsaT042426.1
Chr8 CDS 21818564 21818728 21818564 ID=cds.MsaT042426.1;Parent=MsaT042426.1
Chr8 exon 21819128 21819451 21819128 ID=MsaT042426.1.exon5;Parent=MsaT042426.1
Chr8 CDS 21819128 21819451 21819128 ID=cds.MsaT042426.1;Parent=MsaT042426.1
Gene Sequence

>MsaT042426.1

ATGCCAGGGAAACCAGAAACTGATGATTGGAGAATAAAACATAGTGAGCAGGTTCAGTTGCCATCTTCCATTTACTCTCATCATCAGCCTTGGTGGCTTGAAAATGGCTCCAAACCATCTTCAACTGATCAGCTAAATAGTTCAATCATGAATGATGTCTCACATTCAGAGCCTTTGGTTTCTCCTTCACTTTCTCTAACTGATAACTCAGGTGGGGACGTTGCCAAGGAGCACCGAAACATCAAACATGCCCTGTCCTCAACCGCATTTACCATGGACAAACACCTTGGTCCAAATTCGCAGACGGAACTTGTTGGTCATTCAATTGTTTTAACATCTCCTTATTCAAATGCACAATTCGGTCAAATCTTGAATGCTTATGGACAACAAGCTATGATGAACCCTCAGCTATACCAAATGCATCATGCTAGAATGCTTTTGCCGCTTAAAATGGAAGAGGAACCTGTGTATGTCAATGCAAAGCAGTATCATGGTATTTTGAGACGAAGACAATCGCGTGCAAAGGCTGAGCTTGAAAAGAAAGTTATTAAAGTTAGAAAGCCATATCTTCATGAATCCCGTCACCAACATGCTTTGAGAAGGGCAAGGGGAAATGGCGGTCGTTTCCTCAATACAAAGAAGCTCGAACACAACGATTCTAATGCTGCTCTAGAAAAAGGCAACAATACTGGTGCAACATCGGGTACCAACAATGAGAATCAAGGCTCATCTAATGTGTCGCAGCACATGCACAAAATGCAGGGTTTCAACATCAGTTACCATGATGGAAATGACTTTACAGCACTATGTCATTCACAGGCAAATGGAAAACAGGAGGGTGACTTCTTTGGTAAAAAGATTGAATCAAATGGTGCTATTAAATGA
Protein sequence

>MsaT042426.1

MPGKPETDDWRIKHSEQVQLPSSIYSHHQPWWLENGSKPSSTDQLNSSIMNDVSHSEPLVSPSLSLTDNSGGDVAKEHRNIKHALSSTAFTMDKHLGPNSQTELVGHSIVLTSPYSNAQFGQILNAYGQQAMMNPQLYQMHHARMLLPLKMEEEPVYVNAKQYHGILRRRQSRAKAELEKKVIKVRKPYLHESRHQHALRRARGNGGRFLNTKKLEHNDSNAALEKGNNTGATSGTNNENQGSSNVSQHMHKMQGFNISYHDGNDFTALCHSQANGKQEGDFFGKKIESNGAIK*