AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042434

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042434 MtrunA17_Chr8g0352361 95.429 875 17 3 18 869 19 893 0.0 1696
MsaG042434 MtrunA17_Chr2g0296421 79.648 796 126 7 109 869 248 1042 0.0 1278
MsaG042434 MtrunA17_Chr2g0296421 55.128 156 56 3 10 154 5 157 8.91e-40 159
MsaG042434 MtrunA17_Chr1g0191141 31.785 689 390 19 212 828 378 1058 7.23e-91 311
MsaG042434 MtrunA17_Chr7g0224051 31.395 688 388 19 219 828 391 1072 4.54e-87 301
MsaG042434 MtrunA17_Chr3g0114161 30.836 694 391 19 215 828 519 1203 1.09e-86 301
MsaG042434 MtrunA17_Chr7g0225791 31.040 625 365 11 258 828 628 1240 9.37e-85 296
MsaG042434 MtrunA17_Chr5g0437621 30.453 706 393 22 205 828 570 1259 1.91e-81 286
MsaG042434 MtrunA17_Chr7g0233021 31.669 641 347 21 258 828 567 1186 5.75e-81 285
MsaG042434 MtrunA17_Chr4g0047081 30.635 630 361 14 258 828 578 1190 5.10e-80 282
MsaG042434 MtrunA17_Chr7g0232971 29.922 645 356 17 258 828 630 1252 8.60e-78 276
MsaG042434 MtrunA17_Chr7g0233031 30.327 643 347 23 258 828 586 1199 2.22e-73 263
MsaG042434 MtrunA17_Chr7g0233001 31.612 484 288 9 377 828 3 475 1.12e-67 234
MsaG042434 MtrunA17_Chr7g0232991 29.803 557 307 14 339 828 75 614 5.31e-65 231
MsaG042434 MtrunA17_Chr2g0320781 38.961 231 135 6 588 814 5 233 2.51e-35 134
MsaG042434 MtrunA17_Chr4g0052341 24.911 562 319 22 339 863 747 1242 7.04e-31 131
MsaG042434 MtrunA17_Chr3g0137091 25.000 576 349 20 285 826 232 758 2.64e-30 129
MsaG042434 MtrunA17_Chr5g0393461 25.000 548 332 16 325 826 172 686 7.25e-30 127
MsaG042434 MtrunA17_Chr8g0384531 24.873 591 357 19 330 874 377 926 4.91e-27 119
MsaG042434 MtrunA17_Chr1g0190391 27.570 214 143 3 331 532 576 789 8.78e-26 114
MsaG042434 MtrunA17_Chr1g0163011 22.472 534 346 17 337 820 195 710 6.29e-25 111
MsaG042434 MtrunA17_Chr5g0394201 24.536 485 321 17 333 811 359 804 7.05e-25 111
MsaG042434 MtrunA17_Chr1g0183681 25.651 499 310 17 358 824 410 879 1.65e-24 110
MsaG042434 MtrunA17_Chr1g0205891 24.949 493 310 19 333 811 372 818 4.68e-24 109
MsaG042434 MtrunA17_Chr5g0442141 25.540 509 293 16 334 812 614 1066 5.51e-24 109
MsaG042434 MtrunA17_Chr5g0403921 23.077 533 334 16 334 838 181 665 1.76e-20 97.1
MsaG042434 MtrunA17_Chr4g0066651 24.820 556 321 24 333 849 985 1482 2.35e-20 97.8
MsaG042434 MtrunA17_Chr3g0095091 24.165 509 300 18 334 812 568 1020 3.81e-17 87.0
MsaG042434 MtrunA17_Chr4g0040301 34.132 167 94 5 689 846 394 553 3.90e-17 86.3
MsaG042434 MtrunA17_Chr7g0248031 24.561 513 290 22 334 805 991 1447 3.98e-17 87.0
MsaG042434 MtrunA17_Chr1g0190381 34.868 152 90 2 682 824 195 346 7.48e-17 85.1
MsaG042434 MtrunA17_Chr7g0232121 22.767 571 336 25 337 860 39 551 6.60e-15 79.3
MsaG042434 MtrunA17_Chr1g0175191 34.109 129 80 1 683 811 1070 1193 1.95e-14 78.2
MsaG042434 MtrunA17_Chr8g0348721 23.307 502 309 23 334 805 984 1439 1.14e-13 75.9
MsaG042434 MtrunA17_Chr1g0168031 33.103 145 92 1 677 821 989 1128 7.07e-13 72.8
MsaG042434 MtrunA17_Chr7g0224021 37.500 88 42 2 331 407 25 110 1.34e-12 65.5
MsaG042434 MtrunA17_Chr2g0281601 36.585 123 73 2 689 811 1146 1263 6.16e-12 70.1
MsaG042434 MtrunA17_Chr1g0212331 33.333 126 78 3 687 811 880 1000 2.29e-11 68.2
MsaG042434 MtrunA17_Chr7g0255821 36.220 127 74 3 688 812 663 784 3.68e-11 67.0
MsaG042434 MtrunA17_Chr2g0320751 34.351 131 79 3 682 811 857 981 7.55e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042434 AT2G16390.2 57.358 863 329 12 38 869 37 891 0.0 977
MsaG042434 AT2G16390.1 57.358 863 329 12 38 869 34 888 0.0 976
MsaG042434 AT2G21450.2 46.370 854 381 10 38 871 27 823 0.0 749
MsaG042434 AT2G21450.1 54.235 673 284 5 219 871 148 816 0.0 748
MsaG042434 AT2G21450.3 54.235 673 284 5 219 871 57 725 0.0 748
MsaG042434 AT1G05490.1 31.384 701 379 21 209 828 674 1353 2.24e-87 305
MsaG042434 AT3G24340.1 30.804 659 374 16 222 828 453 1081 6.01e-82 287
MsaG042434 AT3G42670.2 29.142 676 408 16 220 828 539 1210 1.38e-78 278
MsaG042434 AT3G42670.3 29.142 676 408 16 220 828 539 1210 1.38e-78 278
MsaG042434 AT3G42670.1 29.142 676 408 16 220 828 539 1210 1.38e-78 278
MsaG042434 AT3G42670.4 28.951 677 410 15 220 828 539 1212 4.36e-78 277
MsaG042434 AT5G20420.1 28.801 684 415 16 220 836 545 1223 5.49e-77 274
MsaG042434 AT3G32330.1 39.067 343 174 3 315 652 1 313 1.24e-70 237
MsaG042434 AT3G32280.1 35.145 276 130 2 256 531 248 474 8.54e-51 186
MsaG042434 AT1G08600.4 26.736 576 326 16 331 824 712 1273 1.05e-47 185
MsaG042434 AT1G08600.3 26.736 576 326 16 331 824 712 1273 1.05e-47 185
MsaG042434 AT1G08600.2 26.736 576 326 16 331 824 712 1273 1.05e-47 185
MsaG042434 AT1G08600.1 26.307 574 310 17 331 824 712 1252 1.41e-40 162
MsaG042434 AT3G31900.1 32.014 278 129 5 256 533 153 370 7.11e-38 147
MsaG042434 AT5G44800.1 25.704 533 304 19 340 840 692 1164 1.41e-31 134
MsaG042434 AT5G63950.1 27.011 522 307 19 334 812 374 864 2.09e-28 123
MsaG042434 AT5G63950.2 26.408 515 302 18 334 812 374 847 2.42e-27 120
MsaG042434 AT4G31900.2 25.241 519 307 18 337 836 184 640 3.09e-26 116
MsaG042434 AT4G31900.1 25.241 519 307 18 337 836 225 681 5.82e-26 115
MsaG042434 AT4G31900.3 25.241 519 307 18 337 836 225 681 5.94e-26 115
MsaG042434 AT2G13370.1 25.400 500 297 15 337 812 625 1072 3.66e-25 113
MsaG042434 AT2G13370.2 25.400 500 297 15 337 812 625 1072 3.66e-25 113
MsaG042434 AT2G13370.3 25.400 500 297 15 337 812 625 1072 3.66e-25 113
MsaG042434 AT2G25170.1 23.211 517 323 16 337 826 273 742 1.13e-23 108
MsaG042434 AT2G25170.4 23.211 517 323 16 337 826 273 742 1.13e-23 108
MsaG042434 AT2G25170.2 23.211 517 323 16 337 826 290 759 1.14e-23 108
MsaG042434 AT2G25170.3 23.211 517 323 16 337 826 299 768 1.33e-23 108
MsaG042434 AT2G02090.1 22.564 585 358 21 337 860 213 763 5.61e-22 102
MsaG042434 AT2G18760.3 24.798 496 316 15 358 824 463 930 1.86e-21 101
MsaG042434 AT2G18760.4 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G18760.1 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G18760.2 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G18760.7 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G18760.5 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G18760.6 24.798 496 316 15 358 824 404 871 2.82e-21 100
MsaG042434 AT2G28290.2 25.047 535 335 21 334 844 751 1243 7.49e-21 99.8
MsaG042434 AT2G28290.3 25.047 535 335 21 334 844 751 1243 8.23e-21 99.8
MsaG042434 AT2G28290.4 25.047 535 335 21 334 844 751 1243 8.23e-21 99.8
MsaG042434 AT2G28290.1 25.047 535 335 21 334 844 751 1243 8.24e-21 99.8
MsaG042434 AT2G28290.5 25.047 535 335 21 334 844 752 1244 8.31e-21 99.8
MsaG042434 AT2G28290.6 25.047 535 335 21 334 844 752 1244 8.44e-21 99.8
MsaG042434 AT3G06400.2 24.699 498 308 17 336 820 188 631 3.45e-20 97.1
MsaG042434 AT3G06400.1 24.699 498 308 17 336 820 188 631 3.85e-20 97.1
MsaG042434 AT3G06400.3 24.699 498 308 17 336 820 188 631 3.86e-20 97.1
MsaG042434 AT5G66750.1 23.897 544 370 17 280 810 143 655 1.71e-19 94.4
MsaG042434 AT3G19210.3 30.131 229 132 6 318 532 159 373 1.97e-18 91.3
MsaG042434 AT3G19210.3 27.211 147 101 2 681 826 541 682 3.31e-11 67.8
MsaG042434 AT3G19210.2 30.131 229 132 6 318 532 159 373 3.49e-18 90.5
MsaG042434 AT3G19210.2 27.211 147 101 2 681 826 539 680 2.59e-11 68.2
MsaG042434 AT3G19210.1 30.131 229 132 6 318 532 159 373 3.90e-18 90.5
MsaG042434 AT3G19210.1 27.211 147 101 2 681 826 541 682 2.66e-11 68.2
MsaG042434 AT1G03750.1 40.650 123 68 2 689 811 542 659 3.99e-17 87.0
MsaG042434 AT1G03750.1 26.961 204 118 6 340 532 142 325 4.96e-12 70.5
MsaG042434 AT2G46020.5 24.502 502 305 19 333 805 977 1433 8.16e-17 86.7
MsaG042434 AT2G46020.1 24.502 502 305 19 333 805 977 1433 8.16e-17 86.7
MsaG042434 AT2G46020.6 24.502 502 305 19 333 805 977 1433 8.16e-17 86.7
MsaG042434 AT2G46020.2 24.802 504 302 20 333 805 977 1434 1.27e-16 85.9
MsaG042434 AT2G46020.3 24.802 504 302 20 333 805 977 1434 1.27e-16 85.9
MsaG042434 AT2G46020.4 24.802 504 302 20 333 805 977 1434 1.27e-16 85.9
MsaG042434 AT5G43530.1 36.364 132 79 2 690 821 1126 1252 7.90e-15 79.7
MsaG042434 AT3G12810.2 29.082 196 115 4 690 869 1086 1273 3.69e-14 77.8
MsaG042434 AT3G12810.1 29.082 196 115 4 690 869 1090 1277 3.92e-14 77.8
MsaG042434 AT1G61140.1 35.246 122 74 1 690 811 1122 1238 5.08e-12 70.9
MsaG042434 AT1G61140.3 35.246 122 74 1 690 811 964 1080 5.21e-12 70.5
MsaG042434 AT1G11100.5 35.246 122 74 2 690 811 927 1043 5.65e-12 70.5
MsaG042434 AT1G11100.3 35.246 122 74 2 690 811 971 1087 6.51e-12 70.1
MsaG042434 AT1G11100.1 35.246 122 74 2 690 811 1070 1186 6.87e-12 70.1
MsaG042434 AT1G11100.2 35.246 122 74 2 690 811 1113 1229 7.95e-12 70.1
MsaG042434 AT1G11100.4 35.246 122 74 2 690 811 1114 1230 8.22e-12 70.1
MsaG042434 AT5G05130.1 33.544 158 96 4 688 842 722 873 2.05e-11 68.6

Find 187 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATTGCCTTTGAAATATT+TGG 0.058372 8:-21932655 MsaT042434.1:CDS
AAAGAAATCTTCGTGATTTC+AGG 0.179731 8:-21932594 MsaT042434.1:CDS
CTGCAATTGGCTTCAACTTT+GGG 0.188534 8:+21932829 None:intergenic
TCTTTCCTTGGGTTATCTTT+AGG 0.200644 8:+21935423 None:intergenic
ATATCTGTATTCTCATTTCT+TGG 0.223332 8:+21933299 None:intergenic
AGCATCTTCTTCCTCTTCTT+CGG 0.264328 8:+21934460 None:intergenic
ATGACGGGCTAGAGGATATT+TGG 0.265424 8:-21935344 MsaT042434.1:CDS
GATTGCTGAAGAAGATTTCT+TGG 0.265431 8:-21933807 MsaT042434.1:CDS
CGTGAAACGCTTTGACGATT+TGG 0.276231 8:-21933099 MsaT042434.1:CDS
AGTTTAGAGGGTGGAGAATA+TGG 0.279708 8:-21938372 MsaT042434.1:CDS
GTATGGTGATCTTCCTCTTC+TGG 0.293249 8:+21932330 None:intergenic
TCTGCAATTGGCTTCAACTT+TGG 0.296121 8:+21932828 None:intergenic
ACGTAAAAGCTCTGTATAAA+AGG 0.298646 8:-21932170 MsaT042434.1:CDS
GCCTATCGTCCGACGCATTC+AGG 0.299068 8:-21933141 MsaT042434.1:CDS
GTAGTAGTGAAGAACTTTAC+TGG 0.300350 8:+21933004 None:intergenic
CTCAACTTCAATCTTCTGCC+TGG 0.320090 8:+21932917 None:intergenic
GCATCTTCTTCCTCTTCTTC+GGG 0.321372 8:+21934461 None:intergenic
AACAAATTCAAAGGGATGTT+AGG 0.324295 8:-21938185 MsaT042434.1:CDS
ACTCCTTCTTGAAACAAGTA+TGG 0.324325 8:+21932313 None:intergenic
CTCTTTATCAAAATCATGTT+AGG 0.326043 8:-21933223 MsaT042434.1:CDS
CTTTCAAGAATCGATCAATA+AGG 0.330628 8:+21938271 None:intergenic
TTTGTTCTAAAAGACGATCT+TGG 0.336791 8:-21934422 MsaT042434.1:CDS
CTTGTATTGAAACTCAAATA+TGG 0.336843 8:+21934355 None:intergenic
GCGAAGGGATTTCATTGGTT+GGG 0.338037 8:-21932479 MsaT042434.1:CDS
AATGCAGGAGACTGCCGTTT+TGG 0.338469 8:+21938045 None:intergenic
TCATCACATTTCTCTGCAAT+TGG 0.344619 8:+21932816 None:intergenic
AGATCAAAATGTCATAGATT+TGG 0.353547 8:-21938214 MsaT042434.1:CDS
ATCCATGATATGATCAGATA+TGG 0.358045 8:+21932788 None:intergenic
ATTCAGACGAGGAGGATGAT+AGG 0.362438 8:-21938107 MsaT042434.1:CDS
TAAACACCTTTATCTTTCCT+TGG 0.364500 8:+21935411 None:intergenic
TTTCTCGAGAGCCCATTGTT+AGG 0.368522 8:-21932198 MsaT042434.1:CDS
CAACTTTCTCGTCAGAAACT+TGG 0.374377 8:-21933720 MsaT042434.1:CDS
TCTGAATCAATTATAACAAC+AGG 0.376009 8:+21938123 None:intergenic
ATGAAAGTCTTTCCCGATCC+TGG 0.391179 8:+21933659 None:intergenic
ACTCCAACCCTTCCACTTCA+TGG 0.391297 8:+21932623 None:intergenic
AAAGATAAAGGTGTTTATCT+TGG 0.394549 8:-21935405 MsaT042434.1:CDS
ATGAGCCAGGATGCATCCCC+CGG 0.399794 8:+21933685 None:intergenic
AGCGTTTCACGCTAGGTATA+TGG 0.400878 8:+21933111 None:intergenic
GGTAGGATATTTAGCAAAGT+AGG 0.403325 8:+21938293 None:intergenic
TTCTCGAGAGCCCATTGTTA+GGG 0.407823 8:-21932197 MsaT042434.1:CDS
GATAACCCAAGGAAAGATAA+AGG 0.408681 8:-21935417 MsaT042434.1:CDS
GATATGTTTGTCGTGTTTGT+GGG 0.412548 8:-21934400 MsaT042434.1:CDS
TGCATCCTGGCTCATGCTCC+AGG 0.412800 8:-21933677 MsaT042434.1:CDS
TTCTTTGGCTTTCGACGAAT+CGG 0.413140 8:+21933853 None:intergenic
ACTTGGTGGGAGACCATCCC+GGG 0.418803 8:-21933703 MsaT042434.1:CDS
GTTTGTGGGGTTATTGATAG+AGG 0.420352 8:-21934386 MsaT042434.1:CDS
TTTCCTAGGTGTTTGTACTT+TGG 0.423941 8:+21933262 None:intergenic
CGAAGGGATTTCATTGGTTG+GGG 0.434800 8:-21932478 MsaT042434.1:CDS
TCTTCCTCTTCTTCGGGCGG+AGG 0.438149 8:+21934467 None:intergenic
CATATTTGAGTTTCAATACA+AGG 0.440503 8:-21934354 MsaT042434.1:intron
CCGATCCTGGAGCATGAGCC+AGG 0.440756 8:+21933672 None:intergenic
AAACACCTTTATCTTTCCTT+GGG 0.441563 8:+21935412 None:intergenic
ACCACTAGAGGTCGAGCATT+GGG 0.442508 8:+21933599 None:intergenic
TCTGTTACTCGTCAAGCTAT+TGG 0.444358 8:-21932417 MsaT042434.1:CDS
GGCATCCCGTGTCATCATCT+TGG 0.446746 8:-21932457 MsaT042434.1:CDS
CCAAGGGAATATTGTCGACT+TGG 0.450678 8:-21933571 MsaT042434.1:CDS
TCTTGACGAGCTTCCTGGCC+TGG 0.451010 8:-21932970 MsaT042434.1:CDS
CCTAGGAAAGTTGTATTGTC+TGG 0.452995 8:-21933248 MsaT042434.1:CDS
CCAGACAATACAACTTTCCT+AGG 0.454063 8:+21933248 None:intergenic
CTGGCTCATGCTCCAGGATC+GGG 0.454771 8:-21933671 MsaT042434.1:CDS
TTACGTCTTCCCCTAACAAT+GGG 0.457199 8:+21932187 None:intergenic
GGATATGTTTGTCGTGTTTG+TGG 0.457879 8:-21934401 MsaT042434.1:CDS
TCGTCAAAGCGTTTCACGCT+AGG 0.459802 8:+21933104 None:intergenic
AAGAAATGAGAATACAGATA+TGG 0.463938 8:-21933297 MsaT042434.1:CDS
GAACTTTAAGCAATATCTCT+TGG 0.466423 8:+21933354 None:intergenic
TTTCAAGAATCGATCAATAA+GGG 0.467494 8:+21938272 None:intergenic
CTTGAAAGTGGTGACAGATC+AGG 0.469249 8:-21938256 MsaT042434.1:CDS
GGCGAAGGGATTTCATTGGT+TGG 0.470114 8:-21932480 MsaT042434.1:CDS
CCAAGTCGACAATATTCCCT+TGG 0.472773 8:+21933571 None:intergenic
ATGAAACCGCACCAAGTTGA+AGG 0.476732 8:-21933746 MsaT042434.1:CDS
TTTGATTAGCATGCCGAGGA+TGG 0.477649 8:+21933768 None:intergenic
AAGTGGAAGGGTTGGAGTCT+CGG 0.479399 8:-21932618 MsaT042434.1:CDS
ACTGTTTGTATCCTAAGAAA+AGG 0.480977 8:+21933426 None:intergenic
AAAACTTCTGTGATTGCATA+CGG 0.482094 8:+21938399 None:intergenic
TCATCTAAAATGAGAATTGA+GGG 0.482745 8:+21933332 None:intergenic
GTAGGATATTTAGCAAAGTA+GGG 0.482794 8:+21938294 None:intergenic
TATTGATCGATTCTTGAAAG+TGG 0.483603 8:-21938268 MsaT042434.1:CDS
TAGGTGTTTGTACTTTGGCA+AGG 0.485081 8:+21933267 None:intergenic
TCGTCCGACGCATTCAGGCT+AGG 0.486785 8:-21933136 MsaT042434.1:CDS
AACCTTTCCAAATATTTCAA+AGG 0.488803 8:+21932648 None:intergenic
CAAGTCGACAATATTCCCTT+GGG 0.488871 8:+21933572 None:intergenic
TGACAGCAGATCTCAGCAAC+TGG 0.489708 8:-21933483 MsaT042434.1:CDS
CTGAACCTATGTGAATCAGC+TGG 0.490694 8:-21932705 MsaT042434.1:CDS
TTCCATATCTGATCATATCA+TGG 0.490782 8:-21932790 MsaT042434.1:CDS
TCAACTTCAATCTTCTGCCT+GGG 0.491267 8:+21932918 None:intergenic
TGCCTTTGAAATATTTGGAA+AGG 0.491523 8:-21932650 MsaT042434.1:CDS
ACAAATTCAAAGGGATGTTA+GGG 0.498282 8:-21938184 MsaT042434.1:CDS
CGTCCGACGCATTCAGGCTA+GGG 0.499368 8:-21933135 MsaT042434.1:CDS
ATGTGGCGAAGGGATTTCAT+TGG 0.500126 8:-21932484 MsaT042434.1:CDS
AGGTGTTTGTACTTTGGCAA+GGG 0.500985 8:+21933268 None:intergenic
GGATGAGGAACAAATTCAAA+GGG 0.502729 8:-21938193 MsaT042434.1:CDS
CCTGGCTCATGCTCCAGGAT+CGG 0.504936 8:-21933672 MsaT042434.1:CDS
AAACTTAAGTTATCAAGTGA+TGG 0.505358 8:-21938465 MsaT042434.1:CDS
CTCATCTAAAATGAGAATTG+AGG 0.505738 8:+21933331 None:intergenic
TTCCTTGGGTTATCTTTAGG+AGG 0.506209 8:+21935426 None:intergenic
ACCTCTAGTGGTGCTTCCCA+AGG 0.510520 8:-21933588 MsaT042434.1:CDS
TCTGGAGCTGAGTGTTTAGC+TGG 0.512958 8:+21940343 None:intergenic
GCCATGTTCAGCACAAGCTA+AGG 0.525758 8:-21938439 MsaT042434.1:CDS
CACCACTAGAGGTCGAGCAT+TGG 0.525762 8:+21933598 None:intergenic
TCAATCAAAGCATGTGGCGA+AGG 0.527050 8:-21932495 MsaT042434.1:CDS
CTTTCTCGTCAGAAACTTGG+TGG 0.528537 8:-21933717 MsaT042434.1:CDS
AACTTGGTGGGAGACCATCC+CGG 0.528539 8:-21933704 MsaT042434.1:CDS
TCACCATACTTGTTTCAAGA+AGG 0.531321 8:-21932316 MsaT042434.1:CDS
TCTTTATCAAAATCATGTTA+GGG 0.540566 8:-21933222 MsaT042434.1:CDS
TTGATTAGCATGCCGAGGAT+GGG 0.541496 8:+21933769 None:intergenic
TGGATGAGGAACAAATTCAA+AGG 0.542191 8:-21938194 MsaT042434.1:CDS
CCAGGTATTTCGTCTGAGGA+AGG 0.544084 8:-21938495 MsaT042434.1:intron
AGTGTCATCAATGGATTCAT+CGG 0.546019 8:-21940377 None:intergenic
TGACTCCATCTCTCATATCA+AGG 0.547119 8:+21932751 None:intergenic
GCCTGAATGCGTCGGACGAT+AGG 0.547962 8:+21933140 None:intergenic
TTTACGTCTTCCCCTAACAA+TGG 0.549734 8:+21932186 None:intergenic
AAAGTTCTTCACTACTACAA+AGG 0.550973 8:-21932999 MsaT042434.1:CDS
TTCAGACGAGGAGGATGATA+GGG 0.552622 8:-21938106 MsaT042434.1:CDS
AGATCCTCCGCCCGAAGAAG+AGG 0.553398 8:-21934471 MsaT042434.1:CDS
TAGCCATGAAGTGGAAGGGT+TGG 0.555020 8:-21932626 MsaT042434.1:CDS
ACAGTGAAATCTACCAGGCC+AGG 0.556543 8:+21932957 None:intergenic
TTCTCCAGGTATTTCGTCTG+AGG 0.558771 8:-21938499 MsaT042434.1:intron
TTTCCCCAGCTGATTCACAT+AGG 0.559110 8:+21932700 None:intergenic
AGCTGCTGATTCTCCAGAAG+AGG 0.560162 8:-21932343 MsaT042434.1:CDS
GCAGACCTTGATATGAGAGA+TGG 0.560724 8:-21932756 MsaT042434.1:CDS
TGGAAAGGTTAGCCATGAAG+TGG 0.563673 8:-21932635 MsaT042434.1:CDS
GAAAGTTGAATCCTTCAACT+TGG 0.564597 8:+21933735 None:intergenic
TGTTTATCTTGGTGTACAAG+AGG 0.574886 8:-21935394 MsaT042434.1:CDS
ACAACAGGGCATGGTGCCGC+AGG 0.575262 8:+21938138 None:intergenic
GGTTGAGAATACATTACAGA+AGG 0.577283 8:-21933078 MsaT042434.1:CDS
CGAATCGGACATATAAGTCC+TGG 0.578347 8:+21933868 None:intergenic
ACGGGCTAGAGGATATTTGG+AGG 0.579451 8:-21935341 MsaT042434.1:CDS
CTGACATATCAGCCCATCCT+CGG 0.580590 8:-21933781 MsaT042434.1:CDS
AAGGTTAGCCATGAAGTGGA+AGG 0.584042 8:-21932631 MsaT042434.1:CDS
AGAATATGGTAGTGTGACAA+AGG 0.585823 8:-21938358 MsaT042434.1:CDS
TCAATTATAACAACAGGGCA+TGG 0.588679 8:+21938129 None:intergenic
TGAAGGTTAAAAGGAGCACC+AGG 0.589607 8:-21933886 MsaT042434.1:intron
TGGTTGATGCTGAAGATGAC+GGG 0.589842 8:-21935359 MsaT042434.1:CDS
ACCTTAGCTTGTGCTGAACA+TGG 0.590439 8:+21938438 None:intergenic
TCCCAATGCTCGACCTCTAG+TGG 0.592202 8:-21933600 MsaT042434.1:CDS
TGAACCTATGTGAATCAGCT+GGG 0.593499 8:-21932704 MsaT042434.1:CDS
TGAAAACTCTCTTTGTTCAG+CGG 0.593795 8:+21932563 None:intergenic
TCAATGTTCTCAATCTTGTG+AGG 0.593862 8:-21933193 MsaT042434.1:CDS
TTCATTTGATTAGCATGCCG+AGG 0.593888 8:+21933764 None:intergenic
AGAGTTTCAAACGTGGCAAG+TGG 0.594177 8:-21933543 MsaT042434.1:CDS
TGGTGCTCAAACTTACGCCC+AGG 0.602478 8:-21932935 MsaT042434.1:CDS
TTTGGCTCAATCAAAGCATG+TGG 0.603881 8:-21932501 MsaT042434.1:CDS
TCTTCTTCCTCTTCTTCGGG+CGG 0.607633 8:+21934464 None:intergenic
CAATCAAAGCATGTGGCGAA+GGG 0.608230 8:-21932494 MsaT042434.1:CDS
AAGAATCGATCAATAAGGGT+AGG 0.608375 8:+21938276 None:intergenic
TTTCTCGTCAGAAACTTGGT+GGG 0.610600 8:-21933716 MsaT042434.1:CDS
AAATGTCATAGATTTGGATG+AGG 0.611024 8:-21938208 MsaT042434.1:CDS
GTGGTTGATGCTGAAGATGA+CGG 0.612200 8:-21935360 MsaT042434.1:CDS
TTATCAAAATCATGTTAGGG+AGG 0.612213 8:-21933219 MsaT042434.1:CDS
ATTACTATTATCACACACAA+TGG 0.613137 8:+21933397 None:intergenic
ATAGCTTGACGAGTAACAGA+TGG 0.614442 8:+21932420 None:intergenic
CCTCTAGTGGTGCTTCCCAA+GGG 0.616113 8:-21933587 MsaT042434.1:CDS
TGCTGAAGATGACGGGCTAG+AGG 0.620033 8:-21935352 MsaT042434.1:CDS
TTGAATCCTTCAACTTGGTG+CGG 0.621581 8:+21933740 None:intergenic
TATTTGGAGGGAGATGTCAA+TGG 0.623139 8:-21935328 MsaT042434.1:CDS
TTTGAATGGAATGAATATTG+TGG 0.623573 8:-21932273 MsaT042434.1:CDS
TCTCGAGAGCCCATTGTTAG+GGG 0.624813 8:-21932196 MsaT042434.1:CDS
CTATGATCTATACACTGTGA+AGG 0.627350 8:-21933507 MsaT042434.1:CDS
GAACATCCAAGATGATGACA+CGG 0.628032 8:+21932451 None:intergenic
GTGTTTCAACTTCAAATGCG+CGG 0.628851 8:+21932247 None:intergenic
TAGCTTGACGAGTAACAGAT+GGG 0.629929 8:+21932421 None:intergenic
TGCCTCCTAAAGATAACCCA+AGG 0.631547 8:-21935428 MsaT042434.1:CDS
GCTAAACACTCAGCTCCAGA+TGG 0.632896 8:-21940340 MsaT042434.1:intron
CGGGCTAGAGGATATTTGGA+GGG 0.633157 8:-21935340 MsaT042434.1:CDS
TATAATTGATTCAGACGAGG+AGG 0.633443 8:-21938115 MsaT042434.1:CDS
GCACCACAGTGAAATCTACC+AGG 0.638762 8:+21932952 None:intergenic
CAGCAGATCTCAGCAACTGG+AGG 0.639131 8:-21933480 MsaT042434.1:CDS
CCCTTGGGAAGCACCACTAG+AGG 0.642587 8:+21933587 None:intergenic
AACTGGAGGTGTTAAAACAA+TGG 0.644165 8:-21933466 MsaT042434.1:CDS
CTGAATCAATTATAACAACA+GGG 0.647689 8:+21938124 None:intergenic
AGGTTAGCCATGAAGTGGAA+GGG 0.648273 8:-21932630 MsaT042434.1:CDS
ATATGTTTGTCGTGTTTGTG+GGG 0.652598 8:-21934399 MsaT042434.1:CDS
ACAAGAGGAAGATGATAATG+TGG 0.657542 8:-21935379 MsaT042434.1:CDS
GAAGTTTAACAATTCACCTG+AGG 0.658535 8:-21932535 MsaT042434.1:CDS
GGACCCTAGCCTGAATGCGT+CGG 0.659146 8:+21933132 None:intergenic
ACACTTGATGTGAAAGAATG+TGG 0.659776 8:-21932228 MsaT042434.1:CDS
AACATCCAAGATGATGACAC+GGG 0.663891 8:+21932452 None:intergenic
TAGGATATTTAGCAAAGTAG+GGG 0.668264 8:+21938295 None:intergenic
AAGAGAAAAGGATGTTCCTG+CGG 0.672492 8:-21938154 MsaT042434.1:CDS
TTGCCAAAGTACAAACACCT+AGG 0.673997 8:-21933265 MsaT042434.1:CDS
TGTTATAATTGATTCAGACG+AGG 0.676503 8:-21938118 MsaT042434.1:CDS
CTTGGTGGGAGACCATCCCG+GGG 0.683274 8:-21933702 MsaT042434.1:CDS
GAACCTATGTGAATCAGCTG+GGG 0.691335 8:-21932703 MsaT042434.1:CDS
ACAGTGTATAGATCATAGAG+CGG 0.752873 8:+21933512 None:intergenic
GGAAGAAAGAGTTTCAAACG+TGG 0.757529 8:-21933550 MsaT042434.1:CDS
TGGCCTGGTAGATTTCACTG+TGG 0.783766 8:-21932955 MsaT042434.1:CDS
TTGGTGGGAGACCATCCCGG+GGG 0.791439 8:-21933701 MsaT042434.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 21932107 21940389 21932107 ID=MsaG042434
Chr8 mRNA 21932107 21940389 21932107 ID=MsaT042434.1;Parent=MsaG042434
Chr8 exon 21932107 21933903 21932107 ID=MsaT042434.1.exon5;Parent=MsaT042434.1
Chr8 CDS 21932107 21933903 21932107 ID=cds.MsaT042434.1;Parent=MsaT042434.1
Chr8 exon 21934355 21934504 21934355 ID=MsaT042434.1.exon4;Parent=MsaT042434.1
Chr8 CDS 21934355 21934504 21934355 ID=cds.MsaT042434.1;Parent=MsaT042434.1
Chr8 exon 21935311 21935476 21935311 ID=MsaT042434.1.exon3;Parent=MsaT042434.1
Chr8 CDS 21935311 21935476 21935311 ID=cds.MsaT042434.1;Parent=MsaT042434.1
Chr8 exon 21938006 21938513 21938006 ID=MsaT042434.1.exon2;Parent=MsaT042434.1
Chr8 CDS 21938006 21938513 21938006 ID=cds.MsaT042434.1;Parent=MsaT042434.1
Chr8 exon 21940341 21940389 21940341 ID=MsaT042434.1.exon1;Parent=MsaT042434.1
Chr8 CDS 21940341 21940389 21940341 ID=cds.MsaT042434.1;Parent=MsaT042434.1
Gene Sequence

>MsaT042434.1

ATGGATTCATCGGTTCAGTTGTTACCAGCTAAACACTCAGCTCCAGATGGTATTTCGTCTGAGGAAGGATGCAAAAAACTTAAGTTATCAAGTGATGGTTTGCCATGTTCAGCACAAGCTAAGGTTGTTGATTACAGTAATCCGTATGCAATCACAGAAGTTTTAAATAGTTTAGAGGGTGGAGAATATGGTAGTGTGACAAAGGATATTGATGCCCTTATAACCCGAAAAATGCAAGTATTAGGCCCCTACTTTGCTAAATATCCTACCCTTATTGATCGATTCTTGAAAGTGGTGACAGATCAGGATGAAAAAAGTCACCACTCAGATCAAAATGTCATAGATTTGGATGAGGAACAAATTCAAAGGGATGTTAGGGCACCAAAAAGAGAAAAGGATGTTCCTGCGGCACCATGCCCTGTTGTTATAATTGATTCAGACGAGGAGGATGATAGGGATCAGAAGTCTTTTCATGCGTATCATGAAGTTGTTTTGCCAAAACGGCAGTCTCCTGCATTAAAAATGATTGTAAGCTTTGCCCTGCTTTTATTTTTGGACGCGGATAAAAAATTTGAAAAAAAACTGCCTCCTAAAGATAACCCAAGGAAAGATAAAGGTGTTTATCTTGGTGTACAAGAGGAAGATGATAATGTGGTTGATGCTGAAGATGACGGGCTAGAGGATATTTGGAGGGAGATGTCAATGGCAATAGAAACTTCTAAAGATGTTTCTGCAGATCCTCCGCCCGAAGAAGAGGAAGAAGATGCTGACTGCGATCACTCTTTTGTTCTAAAAGACGATCTTGGATATGTTTGTCGTGTTTGTGGGGTTATTGATAGAGGAATTGAAACCATATTTGAGTTTCAATACAAGGTTAAAAGGAGCACCAGGACTTATATGTCCGATTCGTCGAAAGCCAAAGAAAAAGTGGATGCGTTTGGAGTGAAGATTGCTGAAGAAGATTTCTTGGTAACTGACATATCAGCCCATCCTCGGCATGCTAATCAAATGAAACCGCACCAAGTTGAAGGATTCAACTTTCTCGTCAGAAACTTGGTGGGAGACCATCCCGGGGGATGCATCCTGGCTCATGCTCCAGGATCGGGAAAGACTTTCATGATTATCAGTTTCATGCAAAGTTTTCTAGGAAAGTATCCCAATGCTCGACCTCTAGTGGTGCTTCCCAAGGGAATATTGTCGACTTGGAAGAAAGAGTTTCAAACGTGGCAAGTGGAAGATATACCGCTCTATGATCTATACACTGTGAAGGCTGACAGCAGATCTCAGCAACTGGAGGTGTTAAAACAATGGATGAATAACAAAAGTATCCTTTTCTTAGGATACAAACAGTTTTCTTCCATTGTGTGTGATAATAGTAATAACAATGCATCAATATCATGCCAAGAGATATTGCTTAAAGTTCCCTCAATTCTCATTTTAGATGAGGGACACACTCCAAGAAATGAGAATACAGATATGGTACAGTCCCTTGCCAAAGTACAAACACCTAGGAAAGTTGTATTGTCTGGAACTCTTTATCAAAATCATGTTAGGGAGGTTTTCAATGTTCTCAATCTTGTGAGGCCGAAGTTTTTAAAGATGGAAACATCTAAGCCTATCGTCCGACGCATTCAGGCTAGGGTCCATATACCTAGCGTGAAACGCTTTGACGATTTGGTTGAGAATACATTACAGAAGGACCCAGATTTTAAAAGGAAAGTTGCTGTAATTCATGATTTGCGTGAGATGACCAGTAAAGTTCTTCACTACTACAAAGGCGATTTTCTTGACGAGCTTCCTGGCCTGGTAGATTTCACTGTGGTGCTCAAACTTACGCCCAGGCAGAAGATTGAAGTTGAGAAAGCAAAGAAGATGTATATTAGAAAGTTCAAATTTTCTTCTGTAGGCAGTGCAGTGTATCTGCACCCAAAGTTGAAGCCAATTGCAGAGAAATGTGATGAAAATTCCATATCTGATCATATCATGGATGATTTTATAGCAGACCTTGATATGAGAGATGGAGTCAAATCAAAGTTCTTTCGCAATATGCTGAACCTATGTGAATCAGCTGGGGAAAAACTTCTGGTGTTCAGTCAATATCTATTGCCTTTGAAATATTTGGAAAGGTTAGCCATGAAGTGGAAGGGTTGGAGTCTCGGAAAAGAAATCTTCGTGATTTCAGGAGAATCTTCCGCTGAACAAAGAGAGTTTTCAATGGAGAAGTTTAACAATTCACCTGAGGCAAAAATATTTTTTGGCTCAATCAAAGCATGTGGCGAAGGGATTTCATTGGTTGGGGCATCCCGTGTCATCATCTTGGATGTTCATCTCAACCCATCTGTTACTCGTCAAGCTATTGGTCGTGCTTTTCGGCCAGGACAGAAAAAGAAAGTCTTTGTGTATCGTTTGATAGCTGCTGATTCTCCAGAAGAGGAAGATCACCATACTTGTTTCAAGAAGGAGTTAATCTCAAAAATGTGGTTTGAATGGAATGAATATTGTGGTGACCGCGCATTTGAAGTTGAAACACTTGATGTGAAAGAATGTGGTGATCTATTTCTCGAGAGCCCATTGTTAGGGGAAGACGTAAAAGCTCTGTATAAAAGGTTAGACCACTTTTATTTACCTTTTTGCATATCATACGAAAATATGACATTTATATTTTCCTAA
Protein sequence

>MsaT042434.1

MDSSVQLLPAKHSAPDGISSEEGCKKLKLSSDGLPCSAQAKVVDYSNPYAITEVLNSLEGGEYGSVTKDIDALITRKMQVLGPYFAKYPTLIDRFLKVVTDQDEKSHHSDQNVIDLDEEQIQRDVRAPKREKDVPAAPCPVVIIDSDEEDDRDQKSFHAYHEVVLPKRQSPALKMIVSFALLLFLDADKKFEKKLPPKDNPRKDKGVYLGVQEEDDNVVDAEDDGLEDIWREMSMAIETSKDVSADPPPEEEEEDADCDHSFVLKDDLGYVCRVCGVIDRGIETIFEFQYKVKRSTRTYMSDSSKAKEKVDAFGVKIAEEDFLVTDISAHPRHANQMKPHQVEGFNFLVRNLVGDHPGGCILAHAPGSGKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQTWQVEDIPLYDLYTVKADSRSQQLEVLKQWMNNKSILFLGYKQFSSIVCDNSNNNASISCQEILLKVPSILILDEGHTPRNENTDMVQSLAKVQTPRKVVLSGTLYQNHVREVFNVLNLVRPKFLKMETSKPIVRRIQARVHIPSVKRFDDLVENTLQKDPDFKRKVAVIHDLREMTSKVLHYYKGDFLDELPGLVDFTVVLKLTPRQKIEVEKAKKMYIRKFKFSSVGSAVYLHPKLKPIAEKCDENSISDHIMDDFIADLDMRDGVKSKFFRNMLNLCESAGEKLLVFSQYLLPLKYLERLAMKWKGWSLGKEIFVISGESSAEQREFSMEKFNNSPEAKIFFGSIKACGEGISLVGASRVIILDVHLNPSVTRQAIGRAFRPGQKKKVFVYRLIAADSPEEEDHHTCFKKELISKMWFEWNEYCGDRAFEVETLDVKECGDLFLESPLLGEDVKALYKRLDHFYLPFCISYENMTFIFS*