AlfalfaGEDB | MsaG042503

M. sativa ssp. caerulea / MsaG042503

KEGG: K14492
GO: GO:0000156 GO:0000160 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042503	XP_003627814.1	81.356	118	4	1	1	100	1	118	1.35e-59	190

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042503	sp\|O80366\|ARR9_ARATH	57.724	123	26	3	1	100	1	120	3.42e-39	133

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042503	tr\|Q1RU46\|Q1RU46_MEDTR	81.356	118	4	1	1	100	1	118	6.45e-60	190

TFs/TRs:

Gene ID	Type	Classification
MsaG042503	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042503	MtrunA17_Chr8g0352871	81.356	118	4	1	1	100	1	118	1.24e-63	190
MsaG042503	MtrunA17_Chr7g0256551	62.185	119	26	2	1	100	1	119	4.24e-45	145
MsaG042503	MtrunA17_Chr3g0082861	67.257	113	16	3	8	100	3	114	3.88e-44	140
MsaG042503	MtrunA17_Chr4g0021731	60.638	94	11	2	1	76	3	88	2.08e-31	106
MsaG042503	MtrunA17_Chr3g0124861	46.721	122	36	3	6	99	19	139	3.75e-28	102
MsaG042503	MtrunA17_Chr1g0170741	50.893	112	32	3	7	100	6	112	1.72e-26	96.7
MsaG042503	MtrunA17_Chr5g0414931	47.321	112	37	2	7	100	14	121	2.35e-26	97.4
MsaG042503	MtrunA17_Chr3g0128691	47.748	111	30	4	10	100	19	121	1.35e-25	93.2
MsaG042503	MtrunA17_Chr4g0059571	48.276	116	32	3	4	100	21	127	1.97e-25	94.4
MsaG042503	MtrunA17_Chr3g0118211	46.087	115	34	3	4	99	18	123	1.26e-24	92.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG042503	AT3G57040.2	57.724	123	26	3	1	100	1	120	3.47e-40	133
MsaG042503	AT3G57040.1	57.724	123	26	3	1	100	1	120	3.47e-40	133
MsaG042503	AT2G41310.1	55.932	118	34	1	1	100	1	118	5.07e-39	129
MsaG042503	AT1G59940.1	52.679	112	27	3	7	100	31	134	5.83e-29	104
MsaG042503	AT1G59940.2	52.679	112	27	3	7	100	123	226	8.35e-28	103
MsaG042503	AT3G56380.1	43.478	115	40	2	4	100	15	122	2.81e-27	97.8
MsaG042503	AT3G48100.1	46.610	118	36	3	2	100	18	127	6.84e-27	97.8
MsaG042503	AT1G10470.3	50.000	112	30	2	7	100	18	121	7.83e-27	99.0
MsaG042503	AT1G10470.1	50.000	112	30	2	7	100	32	135	9.60e-27	99.4
MsaG042503	AT5G62920.1	49.091	110	30	2	9	100	25	126	1.25e-26	97.1
MsaG042503	AT2G40670.1	45.045	111	38	3	8	100	28	133	2.65e-26	95.9
MsaG042503	AT3G56380.2	43.103	116	40	3	4	100	15	123	1.96e-25	93.2
MsaG042503	AT1G19050.1	45.455	110	34	2	8	99	23	124	2.16e-25	94.4
MsaG042503	AT1G74890.1	45.946	111	34	2	8	100	17	119	3.15e-25	94.0
MsaG042503	AT2G40670.2	44.643	112	38	4	8	100	28	134	1.48e-24	91.3

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTAAGGCCTTGAAATTTCT+TGG	0.292045	8:-23039258	MsaT042503.1:CDS
AGGTATGTCTTTGAAAGATT+TGG	0.295699	8:+23038545	None:intergenic
AGAGAATCAGTTCCATGTTT+TGG	0.307421	8:-23039514	MsaT042503.1:CDS
TCTCACCTATTGATCCTAGA+TGG	0.331221	8:+23038495	None:intergenic
TATTGCTTCAGAAATTAATC+AGG	0.389954	8:-23039199	MsaT042503.1:intron
TGATTAATTTCTGAAGCAAT+AGG	0.390281	8:+23039201	None:intergenic
TACAGTGGATTCTGCTAGTA+AGG	0.426437	8:-23039274	MsaT042503.1:CDS
AAGCAAACCAAGAAATTTCA+AGG	0.430996	8:+23039251	None:intergenic
ATGTTCCATCTAGGATCAAT+AGG	0.432186	8:-23038500	MsaT042503.1:CDS
ATAACAGATTATTGTATGCC+AGG	0.463952	8:-23039065	MsaT042503.1:CDS
GAAATTTCTTGGTTTGCTTG+AGG	0.464582	8:-23039247	MsaT042503.1:CDS
TCTGAGGACATTATCACAAC+AGG	0.486527	8:+23038525	None:intergenic
CCTCAGAGAATGTTCCATCT+AGG	0.494035	8:-23038509	MsaT042503.1:CDS
TATTGTATGCCAGGGATGAC+CGG	0.500365	8:-23039056	MsaT042503.1:CDS
AGATCATAGCCGGTCATCCC+TGG	0.522997	8:+23039047	None:intergenic
TAACAGATTATTGTATGCCA+GGG	0.564090	8:-23039064	MsaT042503.1:CDS
TATCATCAACAGCCAAAACA+TGG	0.580138	8:+23039502	None:intergenic
TATCTTTCAAGTAATGGGCA+TGG	0.581553	8:-23039541	None:intergenic
GTTTGCTTGAGGATGAAATG+AGG	0.583679	8:-23039236	MsaT042503.1:CDS
TTGAAATACAGTTACTACAG+TGG	0.620208	8:-23039289	MsaT042503.1:intron
CCTAGATGGAACATTCTCTG+AGG	0.707424	8:+23038509	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	23038497	23039550	23038497	ID=MsaG042503
Chr8	mRNA	23038497	23039550	23038497	ID=MsaT042503.1;Parent=MsaG042503
Chr8	exon	23038497	23038571	23038497	ID=MsaT042503.1.exon4;Parent=MsaT042503.1
Chr8	CDS	23038497	23038571	23038497	ID=cds.MsaT042503.1;Parent=MsaT042503.1
Chr8	exon	23039031	23039108	23039031	ID=MsaT042503.1.exon3;Parent=MsaT042503.1
Chr8	CDS	23039031	23039108	23039031	ID=cds.MsaT042503.1;Parent=MsaT042503.1
Chr8	exon	23039200	23039300	23039200	ID=MsaT042503.1.exon2;Parent=MsaT042503.1
Chr8	CDS	23039200	23039300	23039200	ID=cds.MsaT042503.1;Parent=MsaT042503.1
Chr8	exon	23039502	23039550	23039502	ID=MsaT042503.1.exon1;Parent=MsaT042503.1
Chr8	CDS	23039502	23039550	23039502	ID=cds.MsaT042503.1;Parent=MsaT042503.1

Gene Sequence	>MsaT042503.1 ATGGGCATGGCTGCAGAGAATCAGTTCCATGTTTTGGCTGTTGATGATATTACTACAGTGGATTCTGCTAGTAAGGCCTTGAAATTTCTTGGTTTGCTTGAGGATGAAATGAGGACTTATGACACTCCTATTGCTTCAGAAATTAATCAGGATGTAGATGTGAATCTGATCATAACAGATTATTGTATGCCAGGGATGACCGGCTATGATCTGTTGAGAAAAATCAAGGAATCCAAATCTTTCAAAGACATACCTGTTGTGATAATGTCCTCAGAGAATGTTCCATCTAGGATCAATAGGTGA
Protein sequence	>MsaT042503.1 MGMAAENQFHVLAVDDITTVDSASKALKFLGLLEDEMRTYDTPIASEINQDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPVVIMSSENVPSRINR*