AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042510 MtrunA17_Chr8g0353111 96.024 327 11 1 1 325 1 327 0.0 660
MsaG042510 MtrunA17_Chr3g0082511 61.224 343 99 16 1 324 1 328 2.51e-113 331
MsaG042510 MtrunA17_Chr5g0441921 44.074 270 124 8 42 296 34 291 1.52e-51 173
MsaG042510 MtrunA17_Chr8g0372461 40.698 172 73 2 109 280 108 250 2.04e-34 128
MsaG042510 MtrunA17_Chr5g0404781 45.679 162 79 4 122 282 100 253 5.18e-33 124
MsaG042510 MtrunA17_Chr1g0155791 42.857 161 79 3 122 282 122 269 8.18e-32 121
MsaG042510 MtrunA17_Chr3g0142171 38.710 186 82 6 122 302 86 244 3.13e-30 116
MsaG042510 MtrunA17_Chr4g0035591 35.632 174 74 3 111 284 9 144 6.28e-26 102
MsaG042510 MtrunA17_Chr2g0281741 62.857 35 13 0 122 156 150 184 1.01e-11 63.2
MsaG042510 MtrunA17_Chr0c01g0489181 54.348 46 21 0 111 156 56 101 1.23e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042510 AT5G12330.1 39.077 325 176 8 1 320 1 308 7.08e-57 187
MsaG042510 AT5G12330.2 39.077 325 176 8 1 320 1 308 7.08e-57 187
MsaG042510 AT5G12330.4 39.077 325 176 8 1 320 88 395 1.94e-56 188
MsaG042510 AT3G54430.1 44.571 175 65 4 120 293 39 182 1.79e-38 135
MsaG042510 AT1G75520.1 44.444 162 83 3 122 280 125 282 2.06e-37 137
MsaG042510 AT5G66350.3 40.223 179 89 1 109 287 107 267 4.14e-37 135
MsaG042510 AT5G66350.2 40.909 176 86 1 109 284 107 264 5.16e-37 135
MsaG042510 AT5G66350.1 40.223 179 89 1 109 287 107 267 5.22e-37 135
MsaG042510 AT3G51060.1 46.875 160 61 5 122 278 144 282 9.32e-35 130
MsaG042510 AT1G19790.2 43.210 162 78 3 122 280 119 269 1.27e-33 127
MsaG042510 AT1G19790.1 43.210 162 78 3 122 280 119 269 1.27e-33 127
MsaG042510 AT4G36260.1 34.361 227 130 5 59 280 32 244 1.66e-30 118
MsaG042510 AT2G18120.1 37.423 163 76 2 122 284 72 208 1.61e-29 112
MsaG042510 AT2G18120.2 37.195 164 73 3 122 284 72 206 1.95e-29 112
MsaG042510 AT2G21400.2 37.654 162 72 3 120 281 8 140 4.77e-29 110
MsaG042510 AT2G21400.4 37.654 162 72 3 120 281 7 139 4.85e-29 110
MsaG042510 AT2G21400.3 35.185 162 61 3 120 281 8 125 5.66e-28 107
MsaG042510 AT2G21400.1 35.185 162 61 3 120 281 7 124 6.25e-28 107
MsaG042510 AT5G12330.3 37.089 213 117 6 1 208 1 201 3.63e-27 106
MsaG042510 AT5G33210.2 70.000 50 15 0 122 171 17 66 2.82e-20 84.7
MsaG042510 AT5G33210.1 70.000 50 15 0 122 171 52 101 2.93e-19 84.0

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAAATGGTGAGTTTA+GGG 0.286532 8:+23201992 MsaT042510.1:CDS
ATATTCTCATTGTTATTGTT+AGG 0.304242 8:-23201840 None:intergenic
GTGAAGAAAATGGTGAGTTT+AGG 0.324594 8:+23201991 MsaT042510.1:CDS
CTATTAGAACCAAGTCTCTT+AGG 0.357173 8:-23201668 None:intergenic
TGAAGATGAACTTGCTTACT+TGG 0.373159 8:+23202950 MsaT042510.1:CDS
AGTGATGGTGGTGCTTCTTC+TGG 0.380721 8:+23202179 MsaT042510.1:CDS
TGTTGCAAAATGAACATGTT+AGG 0.386699 8:+23201645 None:intergenic
GATGAAGGTAAGGGAATGTT+TGG 0.393591 8:-23201750 None:intergenic
TATTCTCATTGTTATTGTTA+GGG 0.402170 8:-23201839 None:intergenic
TCATCATTATTTGTTGTGGT+TGG 0.418207 8:-23201899 None:intergenic
TTGGCTACTGTGAATATTAG+TGG 0.419206 8:+23202969 MsaT042510.1:CDS
ATGTTCTTCCTCTGCAATTG+AGG 0.420350 8:+23203106 MsaT042510.1:CDS
TGCCAACAGCAGATACTCTA+TGG 0.431824 8:-23202923 None:intergenic
CGGCGTCGATGAGGCGGGAC+CGG 0.436701 8:+23202129 MsaT042510.1:CDS
TGTTGGTTGTGAAGTGATGA+AGG 0.446919 8:-23201699 None:intergenic
TGGATTATGATCTGATGAAA+TGG 0.452099 8:-23201730 None:intergenic
TATGATCTGATGAAATGGTT+TGG 0.460879 8:-23201725 None:intergenic
TTTAGGGTTTGTCAAGATTG+TGG 0.461202 8:+23202008 MsaT042510.1:CDS
GAATTGCAACTTGGAAGTAA+TGG 0.469782 8:+23203062 MsaT042510.1:CDS
CAATCTCAACAAGCAGAAGC+AGG 0.470698 8:-23203146 None:intergenic
TAGTGCTGATGATGAAGGTA+AGG 0.474808 8:-23201760 None:intergenic
ATGATGGGGTGTGAAGAAAA+TGG 0.479641 8:+23201981 MsaT042510.1:CDS
TCAGCACTAAGTGTTGGTCT+TGG 0.487088 8:+23201774 MsaT042510.1:CDS
AAAGGGTTTCTCTATGATCA+AGG 0.488489 8:+23203002 MsaT042510.1:CDS
CATGTTAGGCCTAAGAGACT+TGG 0.493330 8:+23201659 MsaT042510.1:CDS
AATCAAAATTCTCTGCCTCT+TGG 0.499143 8:-23202204 None:intergenic
CGTGAAGAGCACGTGGATAC+CGG 0.501925 8:+23202109 MsaT042510.1:CDS
TTCATCATCATTATTTGTTG+TGG 0.510215 8:-23201903 None:intergenic
ATTAGTGGCCATGTATTCAA+AGG 0.512383 8:+23202984 MsaT042510.1:CDS
GAAGCAGGGTAAGCACTAGT+TGG 0.512667 8:-23203131 None:intergenic
ATTGTAGCTATAAAAGATGC+AGG 0.519421 8:+23202048 MsaT042510.1:CDS
CAACTTGGAAGTAATGGAAG+TGG 0.519956 8:+23203068 MsaT042510.1:CDS
GGTGAGTAGTGGTTAGGAGT+GGG 0.529385 8:-23201803 None:intergenic
GGCAAAGTCTGCTTGAAACC+AGG 0.531084 8:-23202867 None:intergenic
AGAGAAACCCTTTGAATACA+TGG 0.531479 8:-23202992 None:intergenic
TTAGTGGCCATGTATTCAAA+GGG 0.533384 8:+23202985 MsaT042510.1:CDS
GCAGGCGCGCGAACATAGCG+AGG 0.545668 8:-23202888 None:intergenic
AGGACTTGTTGTAAAGGTCG+TGG 0.546270 8:+23202068 MsaT042510.1:CDS
GATGAGGGTGAGTAGTGGTT+AGG 0.546291 8:-23201809 None:intergenic
AGATGCAGGACTTGTTGTAA+AGG 0.546985 8:+23202062 MsaT042510.1:CDS
GCTTAAACTTCTTGTTCTAG+TGG 0.556742 8:-23202282 None:intergenic
CTATGGCACTTGAAAACAGC+AGG 0.559201 8:-23202906 None:intergenic
GGGTGAGTAGTGGTTAGGAG+TGG 0.563980 8:-23201804 None:intergenic
GATACCGGCGTCGATGAGGC+GGG 0.565411 8:+23202124 MsaT042510.1:CDS
ACACTTAGTGCTGATGATGA+AGG 0.573973 8:-23201765 None:intergenic
TGTGTTTCAGAATTGCAACT+TGG 0.580875 8:+23203053 MsaT042510.1:CDS
TGCCATAGAGTATCTGCTGT+TGG 0.581569 8:+23202921 MsaT042510.1:CDS
TTGATGATGAGGGTGAGTAG+TGG 0.583936 8:-23201814 None:intergenic
GAGGCGGGACCGGAGTATGA+TGG 0.586244 8:+23202139 MsaT042510.1:CDS
CGGAGTATGATGGTGGCTGA+AGG 0.587151 8:+23202149 MsaT042510.1:CDS
GCTGAAGGTGGTGACAGTGA+TGG 0.591111 8:+23202164 MsaT042510.1:CDS
GGATACCGGCGTCGATGAGG+CGG 0.591264 8:+23202123 MsaT042510.1:CDS
GAAGGTGGTGACAGTGATGG+TGG 0.597594 8:+23202167 MsaT042510.1:CDS
CGTGGATACCGGCGTCGATG+AGG 0.610218 8:+23202120 MsaT042510.1:CDS
AGAGTATCTGCTGTTGGCAA+TGG 0.611315 8:+23202927 MsaT042510.1:CDS
TCATCATCAGCACTAAGTGT+TGG 0.620780 8:+23201768 MsaT042510.1:CDS
AGTGCTGATGATGAAGGTAA+GGG 0.621262 8:-23201759 None:intergenic
AATCTCAACAAGCAGAAGCA+GGG 0.625538 8:-23203145 None:intergenic
GTTGTGGTTGGCACATCTTG+AGG 0.627215 8:-23201887 None:intergenic
CGGTCCCGCCTCATCGACGC+CGG 0.629933 8:-23202128 None:intergenic
GCGGGACCGGAGTATGATGG+TGG 0.640592 8:+23202142 MsaT042510.1:CDS
GTGCTTCTTCTGGTACCAAG+AGG 0.651535 8:+23202189 MsaT042510.1:CDS
CTTCAGCCACCATCATACTC+CGG 0.656848 8:-23202148 None:intergenic
AGTATGATGGTGGCTGAAGG+TGG 0.658861 8:+23202152 MsaT042510.1:CDS
ATAACTAGCTCTTGTCACCA+AGG 0.669802 8:+23202311 MsaT042510.1:CDS
AGTTGGAACCTCAATTGCAG+AGG 0.675832 8:-23203114 None:intergenic
GTACTCACGTGAAGAGCACG+TGG 0.684544 8:+23202102 MsaT042510.1:CDS
AGTTGCAATTCTGAAACACA+AGG 0.685469 8:-23203050 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 23201654 23203163 23201654 ID=MsaG042510
Chr8 mRNA 23201654 23203163 23201654 ID=MsaT042510.1;Parent=MsaG042510
Chr8 exon 23201654 23202332 23201654 ID=MsaT042510.1.exon1;Parent=MsaT042510.1
Chr8 CDS 23201654 23202332 23201654 ID=cds.MsaT042510.1;Parent=MsaT042510.1
Chr8 exon 23202865 23203163 23202865 ID=MsaT042510.1.exon2;Parent=MsaT042510.1
Chr8 CDS 23202865 23203163 23202865 ID=cds.MsaT042510.1;Parent=MsaT042510.1
Gene Sequence

>MsaT042510.1

ATGAACATGTTAGGCCTAAGAGACTTGGTTCTAATAGCTCAAACTCCTTCATCACTTCACAACCAACAAAACCAAACCATTTCATCAGATCATAATCCAAACATTCCCTTACCTTCATCATCAGCACTAAGTGTTGGTCTTGGAATTTTCCCACTCCTAACCACTACTCACCCTCATCATCAAAACCCTAACAATAACAATGAGAATATTCATCAAGACTCGAGTTTTTGGAACCTCAAGATGTGCCAACCACAACAAATAATGATGATGAATTCATCAAGAAAAAGAGTTGAAGAAGAAGAAGATGATGTAAGAAACAAAAACTTGATGATGGGGTGTGAAGAAAATGGTGAGTTTAGGGTTTGTCAAGATTGTGGAAATAGAGCTAAGAAAGATTGTAGCTATAAAAGATGCAGGACTTGTTGTAAAGGTCGTGGTTTTGATTGTAGTACTCACGTGAAGAGCACGTGGATACCGGCGTCGATGAGGCGGGACCGGAGTATGATGGTGGCTGAAGGTGGTGACAGTGATGGTGGTGCTTCTTCTGGTACCAAGAGGCAGAGAATTTTGATTTCATCATCACAGAATAATGCTGCTGCTAACTCTCATAGTTCTAGTTCTAATGCTGCCACTAGAACAAGAAGTTTAAGCTTAGATATAACTAGCTCTTGTCACCAAGATCCTGGTTTCAAGCAGACTTTGCCTCGCTATGTTCGCGCGCCTGCTGTTTTCAAGTGCCATAGAGTATCTGCTGTTGGCAATGGTGAAGATGAACTTGCTTACTTGGCTACTGTGAATATTAGTGGCCATGTATTCAAAGGGTTTCTCTATGATCAAGGTGTTGATGACAAAAATGAAACAATGCCTTGTGTTTCAGAATTGCAACTTGGAAGTAATGGAAGTGGAAAGAGTAATAGAGAATGTTCTTCCTCTGCAATTGAGGTTCCAACTAGTGCTTACCCTGCTTCTGCTTGTTGA

Protein sequence

>MsaT042510.1

MNMLGLRDLVLIAQTPSSLHNQQNQTISSDHNPNIPLPSSSALSVGLGIFPLLTTTHPHHQNPNNNNENIHQDSSFWNLKMCQPQQIMMMNSSRKRVEEEEDDVRNKNLMMGCEENGEFRVCQDCGNRAKKDCSYKRCRTCCKGRGFDCSTHVKSTWIPASMRRDRSMMVAEGGDSDGGASSGTKRQRILISSSQNNAAANSHSSSSNAATRTRSLSLDITSSCHQDPGFKQTLPRYVRAPAVFKCHRVSAVGNGEDELAYLATVNISGHVFKGFLYDQGVDDKNETMPCVSELQLGSNGSGKSNRECSSSAIEVPTSAYPASAC*