Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG042702 | BBN69862.1 | 46.535 | 101 | 38 | 2 | 2 | 86 | 151 | 251 | 2.46e-16 | 82.8 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG042702 | sp|Q9FKK2|AGL62_ARATH | 35.036 | 137 | 41 | 4 | 2 | 90 | 18 | 154 | 2.48e-13 | 66.6 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG042702 | tr|G7L6X5|G7L6X5_MEDTR | 47.368 | 133 | 25 | 3 | 2 | 89 | 20 | 152 | 6.07e-24 | 100 |
Gene ID | Type | Classification |
---|---|---|
MsaG042702 | TF | MADS-M-type |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000143 | MsaG042702 | 0.851191 | 1.990944e-60 | 1.124236e-57 |
MsaG001363 | MsaG042702 | 0.835675 | 2.668344e-56 | 9.120020e-54 |
MsaG002050 | MsaG042702 | 0.825379 | 8.592414e-54 | 2.184072e-51 |
MsaG002113 | MsaG042702 | 0.847380 | 2.264698e-59 | 1.123211e-56 |
MsaG002225 | MsaG042702 | 0.810835 | 1.619774e-50 | 2.823066e-48 |
MsaG003809 | MsaG042702 | 0.825537 | 7.890131e-54 | 2.014333e-51 |
MsaG003997 | MsaG042702 | 0.842919 | 3.589630e-58 | 1.537357e-55 |
MsaG004531 | MsaG042702 | 0.831534 | 2.852288e-55 | 8.627515e-53 |
MsaG004716 | MsaG042702 | 0.866869 | 4.181286e-65 | 4.260011e-62 |
MsaG004889 | MsaG042702 | 0.859840 | 6.145084e-63 | 4.748860e-60 |
MsaG005541 | MsaG042702 | 0.852480 | 8.613514e-61 | 5.088089e-58 |
MsaG006034 | MsaG042702 | 0.821953 | 5.400917e-53 | 1.250935e-50 |
MsaG005924 | MsaG042702 | 0.851898 | 1.258045e-60 | 7.282144e-58 |
MsaG007137 | MsaG042702 | 0.840149 | 1.911655e-57 | 7.497541e-55 |
MsaG007237 | MsaG042702 | 0.838058 | 6.618291e-57 | 2.431712e-54 |
MsaG007255 | MsaG042702 | 0.834502 | 5.255007e-56 | 1.734224e-53 |
MsaG007571 | MsaG042702 | 0.872717 | 5.267723e-67 | 6.854802e-64 |
MsaG011531 | MsaG042702 | 0.815272 | 1.742084e-51 | 3.390894e-49 |
MsaG012173 | MsaG042702 | 0.831787 | 2.472515e-55 | 7.534831e-53 |
MsaG012333 | MsaG042702 | 0.822913 | 3.239523e-53 | 7.699707e-51 |
MsaG012469 | MsaG042702 | 0.869737 | 5.025943e-66 | 5.760481e-63 |
MsaG042702 | MsaG043755 | 0.862443 | 1.000400e-63 | 8.542457e-61 |
MsaG042702 | MsaG045030 | 0.831421 | 3.041256e-55 | 9.168531e-53 |
MsaG042702 | MsaG045598 | 0.805941 | 1.771905e-49 | 2.745959e-47 |
MsaG042702 | MsaG046652 | 0.843959 | 1.899712e-58 | 8.411424e-56 |
MsaG042702 | MsaG046927 | 0.821381 | 7.313969e-53 | 1.668463e-50 |
MsaG042702 | MsaG046943 | 0.841322 | 9.452538e-58 | 3.846878e-55 |
MsaG042702 | MsaG047049 | 0.834485 | 5.305482e-56 | 1.749999e-53 |
MsaG042702 | MsaG002256 | 0.831567 | 2.799308e-55 | 8.475482e-53 |
MsaG042702 | MsaG001975 | 0.833412 | 9.817598e-56 | 3.137026e-53 |
MsaG042702 | MsaG002349 | 0.815106 | 1.895771e-51 | 3.674430e-49 |
MsaG042702 | MsaG008957 | 0.814828 | 2.184108e-51 | 4.203791e-49 |
MsaG042702 | MsaG008556 | 0.883152 | 1.221402e-70 | 2.558461e-67 |
MsaG042702 | MsaG011732 | 0.805593 | 2.095791e-49 | 3.221374e-47 |
MsaG042702 | MsaG023461 | 0.807145 | 9.902117e-50 | 1.578781e-47 |
MsaG042702 | MsaG028857 | 0.850028 | 4.210125e-60 | 2.283807e-57 |
MsaG042702 | MsaG030577 | 0.850912 | 2.383554e-60 | 1.333146e-57 |
MsaG042702 | MsaG033422 | 0.835471 | 3.001834e-56 | 1.019802e-53 |
MsaG042702 | MsaG035322 | 0.824189 | 1.634963e-53 | 4.022626e-51 |
MsaG017137 | MsaG042702 | 0.816007 | 1.197354e-51 | 2.374341e-49 |
MsaG017440 | MsaG042702 | 0.872045 | 8.802874e-67 | 1.112890e-63 |
MsaG017530 | MsaG042702 | 0.845810 | 6.049197e-59 | 2.847007e-56 |
MsaG018527 | MsaG042702 | 0.853927 | 3.329855e-61 | 2.070352e-58 |
MsaG018582 | MsaG042702 | 0.860359 | 4.292128e-63 | 3.383876e-60 |
MsaG018652 | MsaG042702 | 0.808434 | 5.285178e-50 | 8.691037e-48 |
MsaG018662 | MsaG042702 | 0.834327 | 5.811669e-56 | 1.907966e-53 |
MsaG018701 | MsaG042702 | 0.839861 | 2.271589e-57 | 8.829422e-55 |
MsaG019728 | MsaG042702 | 0.836832 | 1.359798e-56 | 4.812455e-54 |
MsaG019974 | MsaG042702 | 0.858655 | 1.388127e-62 | 1.025669e-59 |
MsaG020381 | MsaG042702 | 0.833491 | 9.383018e-56 | 3.005094e-53 |
MsaG020660 | MsaG042702 | 0.872943 | 4.428917e-67 | 5.820399e-64 |
MsaG021588 | MsaG042702 | 0.809119 | 3.778941e-50 | 6.317591e-48 |
MsaG022399 | MsaG042702 | 0.806951 | 1.087757e-49 | 1.726386e-47 |
MsaG023618 | MsaG042702 | 0.841013 | 1.138726e-57 | 4.589110e-55 |
MsaG023694 | MsaG042702 | 0.869145 | 7.811805e-66 | 8.734030e-63 |
MsaG024094 | MsaG042702 | 0.863734 | 4.007511e-64 | 3.600162e-61 |
MsaG026607 | MsaG042702 | 0.806412 | 1.411845e-49 | 2.212576e-47 |
MsaG027083 | MsaG042702 | 0.883574 | 8.556851e-71 | 1.829524e-67 |
MsaG027864 | MsaG042702 | 0.859658 | 6.970655e-63 | 5.349382e-60 |
MsaG028062 | MsaG042702 | 0.872178 | 7.954870e-67 | 1.011498e-63 |
MsaG028106 | MsaG042702 | 0.855464 | 1.200220e-61 | 7.885233e-59 |
MsaG028119 | MsaG042702 | 0.819346 | 2.131147e-52 | 4.607229e-50 |
MsaG028191 | MsaG042702 | 0.805305 | 2.406166e-49 | 3.673797e-47 |
MsaG028276 | MsaG042702 | 0.862938 | 7.049830e-64 | 6.137792e-61 |
MsaG028814 | MsaG042702 | 0.803296 | 6.278534e-49 | 9.149985e-47 |
MsaG028861 | MsaG042702 | 0.862956 | 6.961185e-64 | 6.064667e-61 |
MsaG028877 | MsaG042702 | 0.834347 | 5.743764e-56 | 1.886827e-53 |
MsaG029072 | MsaG042702 | 0.875743 | 5.027193e-68 | 7.472149e-65 |
MsaG029250 | MsaG042702 | 0.851390 | 1.749950e-60 | 9.949920e-58 |
MsaG030112 | MsaG042702 | 0.908809 | 2.734712e-81 | 2.392646e-77 |
MsaG034783 | MsaG042702 | 0.859263 | 9.147589e-63 | 6.916284e-60 |
MsaG036058 | MsaG042702 | 0.861251 | 2.308079e-63 | 1.882020e-60 |
MsaG040389 | MsaG042702 | 0.827381 | 2.881055e-54 | 7.742999e-52 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG042702 | MtrunA17_Chr8g0355091 | 47.368 | 133 | 25 | 3 | 2 | 89 | 20 | 152 | 1.17e-27 | 100 |
MsaG042702 | MtrunA17_Chr1g0206691 | 44.361 | 133 | 29 | 2 | 2 | 89 | 3 | 135 | 9.58e-27 | 97.8 |
MsaG042702 | MtrunA17_Chr1g0206701 | 45.113 | 133 | 28 | 2 | 2 | 89 | 20 | 152 | 1.24e-26 | 97.8 |
MsaG042702 | MtrunA17_Chr1g0206721 | 45.255 | 137 | 29 | 4 | 2 | 92 | 20 | 156 | 1.44e-26 | 97.4 |
MsaG042702 | MtrunA17_Chr2g0292791 | 45.865 | 133 | 27 | 4 | 2 | 89 | 20 | 152 | 2.06e-25 | 94.7 |
MsaG042702 | MtrunA17_Chr1g0149391 | 45.113 | 133 | 28 | 3 | 2 | 89 | 20 | 152 | 4.65e-25 | 93.6 |
MsaG042702 | MtrunA17_Chr3g0099421 | 45.113 | 133 | 28 | 4 | 2 | 89 | 45 | 177 | 2.02e-24 | 92.4 |
MsaG042702 | MtrunA17_Chr1g0206991 | 41.791 | 134 | 33 | 3 | 2 | 90 | 20 | 153 | 7.23e-23 | 86.7 |
MsaG042702 | MtrunA17_Chr3g0089141 | 40.602 | 133 | 34 | 3 | 2 | 89 | 20 | 152 | 1.49e-21 | 84.3 |
MsaG042702 | MtrunA17_Chr1g0208561 | 39.850 | 133 | 35 | 3 | 2 | 89 | 20 | 152 | 8.16e-21 | 82.4 |
MsaG042702 | MtrunA17_Chr2g0325971 | 39.850 | 133 | 35 | 3 | 2 | 89 | 20 | 152 | 1.23e-19 | 79.3 |
MsaG042702 | MtrunA17_Chr1g0208581 | 39.098 | 133 | 36 | 3 | 2 | 89 | 20 | 152 | 8.48e-19 | 77.0 |
MsaG042702 | MtrunA17_Chr1g0188671 | 36.567 | 134 | 40 | 2 | 2 | 90 | 58 | 191 | 1.21e-18 | 77.4 |
MsaG042702 | MtrunA17_Chr5g0419601 | 39.535 | 129 | 33 | 3 | 2 | 85 | 20 | 148 | 1.49e-17 | 73.9 |
MsaG042702 | MtrunA17_Chr2g0317481 | 36.090 | 133 | 40 | 2 | 2 | 89 | 20 | 152 | 1.03e-16 | 72.0 |
MsaG042702 | MtrunA17_Chr2g0317451 | 36.090 | 133 | 40 | 2 | 2 | 89 | 20 | 152 | 1.11e-16 | 71.6 |
MsaG042702 | MtrunA17_Chr1g0187361 | 37.984 | 129 | 35 | 4 | 2 | 85 | 20 | 148 | 2.30e-16 | 70.9 |
MsaG042702 | MtrunA17_Chr5g0422421 | 35.385 | 130 | 39 | 2 | 2 | 86 | 20 | 149 | 3.65e-16 | 70.5 |
MsaG042702 | MtrunA17_Chr3g0119901 | 36.508 | 126 | 33 | 3 | 3 | 81 | 21 | 146 | 5.46e-16 | 69.3 |
MsaG042702 | MtrunA17_Chr3g0085241 | 36.029 | 136 | 36 | 4 | 3 | 90 | 53 | 185 | 8.81e-16 | 69.7 |
MsaG042702 | MtrunA17_Chr3g0099411 | 77.500 | 40 | 9 | 0 | 2 | 41 | 20 | 59 | 2.63e-14 | 65.1 |
MsaG042702 | MtrunA17_Chr3g0111841 | 69.231 | 39 | 12 | 0 | 3 | 41 | 8 | 46 | 1.89e-11 | 54.7 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG042702 | AT5G60440.1 | 35.036 | 137 | 41 | 4 | 2 | 90 | 18 | 154 | 2.52e-14 | 66.6 |
MsaG042702 | AT4G36590.1 | 69.231 | 39 | 12 | 0 | 2 | 40 | 18 | 56 | 1.79e-11 | 58.2 |
Find 18 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AAGCGACCGAGGCTCAATTT+TGG | 0.310911 | 8:-26144693 | MsaT042702.1:CDS |
TGGTGGGCTGGTCCCGTTGA+TGG | 0.316276 | 8:-26144673 | MsaT042702.1:CDS |
GTCCCGTTGATGGGATGAAT+AGG | 0.432754 | 8:-26144663 | MsaT042702.1:CDS |
CCTCGGTCGCTTTGCGCAAG+TGG | 0.449815 | 8:+26144704 | None:intergenic |
GGTGGGCTGGTCCCGTTGAT+GGG | 0.452333 | 8:-26144672 | MsaT042702.1:CDS |
TCCCGTTGATGGGATGAATA+GGG | 0.453040 | 8:-26144662 | MsaT042702.1:CDS |
GCCCTATTCATCCCATCAAC+GGG | 0.481029 | 8:+26144661 | None:intergenic |
ACTGAGAGTGCAAAGCTCAC+TGG | 0.526727 | 8:+26144960 | None:intergenic |
GGCCCTATTCATCCCATCAA+CGG | 0.536262 | 8:+26144660 | None:intergenic |
CTTTCTCGAAGCGCCGTAGT+GGG | 0.553366 | 8:-26144996 | MsaT042702.1:CDS |
CCACTTGCGCAAAGCGACCG+AGG | 0.571740 | 8:-26144704 | MsaT042702.1:CDS |
ACTTTCTCGAAGCGCCGTAG+TGG | 0.571963 | 8:-26144997 | MsaT042702.1:CDS |
AATGAAAATACCTTCTCCCC+AGG | 0.587583 | 8:+26144909 | None:intergenic |
AGCCCACCAAAATTGAGCCT+CGG | 0.589062 | 8:+26144687 | None:intergenic |
GATCAACGATTCATCGGACA+TGG | 0.593206 | 8:-26144754 | MsaT042702.1:CDS |
GACTCAGATCAACGATTCAT+CGG | 0.601432 | 8:-26144760 | MsaT042702.1:CDS |
AGTGAGCTTTGCACTCTCAG+TGG | 0.609659 | 8:-26144958 | MsaT042702.1:CDS |
CTTTCTTGAAGAGCCCACTA+CGG | 0.610979 | 8:+26144983 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr8 | gene | 26144630 | 26145043 | 26144630 | ID=MsaG042702 |
Chr8 | mRNA | 26144630 | 26145043 | 26144630 | ID=MsaT042702.1;Parent=MsaG042702 |
Chr8 | exon | 26144630 | 26144784 | 26144630 | ID=MsaT042702.1.exon2;Parent=MsaT042702.1 |
Chr8 | CDS | 26144630 | 26144784 | 26144630 | ID=cds.MsaT042702.1;Parent=MsaT042702.1 |
Chr8 | exon | 26144920 | 26145043 | 26144920 | ID=MsaT042702.1.exon1;Parent=MsaT042702.1 |
Chr8 | CDS | 26144920 | 26145043 | 26144920 | ID=cds.MsaT042702.1;Parent=MsaT042702.1 |
Gene Sequence |
Protein sequence |