AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042710 MtrunA17_Chr8g0355121 92.857 322 14 1 1 322 1 313 0.0 591
MsaG042710 MtrunA17_Chr3g0080341 64.087 323 109 4 6 322 1 322 5.80e-143 406
MsaG042710 MtrunA17_Chr7g0254991 47.006 334 146 8 6 322 1 320 1.48e-86 263
MsaG042710 MtrunA17_Chr6g0460301 49.398 332 142 7 6 322 1 321 3.05e-83 254
MsaG042710 MtrunA17_Chr4g0044771 36.337 344 144 13 23 301 17 350 2.59e-42 150
MsaG042710 MtrunA17_Chr5g0440841 54.967 151 61 3 1 148 1 147 6.15e-35 128
MsaG042710 MtrunA17_Chr5g0440841 61.364 88 33 1 235 322 183 269 3.08e-24 99.8
MsaG042710 MtrunA17_Chr7g0266211 34.682 346 143 11 30 294 45 388 4.77e-34 129
MsaG042710 MtrunA17_Chr2g0321411 35.587 281 130 12 27 294 11 253 3.69e-30 115
MsaG042710 MtrunA17_Chr2g0321381 37.589 282 142 10 26 294 24 284 4.76e-30 115
MsaG042710 MtrunA17_Chr3g0133971 48.529 136 62 3 12 144 14 144 1.09e-29 117
MsaG042710 MtrunA17_Chr3g0133971 63.768 69 22 1 229 297 333 398 3.69e-17 81.6
MsaG042710 MtrunA17_Chr5g0424871 31.353 303 155 11 16 273 4 298 5.37e-24 99.8
MsaG042710 MtrunA17_Chr4g0051101 53.521 71 29 1 238 304 117 187 4.05e-12 64.3
MsaG042710 MtrunA17_Chr5g0405951 61.017 59 11 1 251 297 166 224 2.47e-11 62.8
MsaG042710 MtrunA17_Chr1g0201751 62.745 51 19 0 246 296 157 207 7.25e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042710 AT3G56850.1 53.055 311 107 8 23 322 15 297 5.46e-90 271
MsaG042710 AT2G41070.3 49.007 302 94 9 23 320 15 260 1.06e-74 231
MsaG042710 AT2G41070.1 49.007 302 94 9 23 320 15 260 1.06e-74 231
MsaG042710 AT2G41070.2 49.007 302 94 9 23 320 15 260 1.06e-74 231
MsaG042710 AT2G41070.4 49.007 302 94 9 23 320 24 269 1.41e-74 231
MsaG042710 AT2G41070.5 49.281 278 81 9 23 296 15 236 5.14e-66 208
MsaG042710 AT2G36270.1 33.092 414 156 15 23 322 36 442 5.37e-38 140
MsaG042710 AT2G36270.2 33.092 414 156 15 23 322 36 442 5.37e-38 140
MsaG042710 AT2G36270.3 33.508 382 140 12 23 297 36 410 8.58e-37 137
MsaG042710 AT1G49720.1 43.407 182 83 5 1 167 1 177 1.38e-31 122
MsaG042710 AT1G49720.1 48.837 86 35 1 246 322 307 392 5.93e-14 72.4
MsaG042710 AT1G49720.3 43.407 182 83 5 1 167 1 177 1.38e-31 122
MsaG042710 AT1G49720.3 48.837 86 35 1 246 322 307 392 5.93e-14 72.4
MsaG042710 AT1G49720.2 43.407 182 83 5 1 167 1 177 1.71e-31 122
MsaG042710 AT1G49720.2 68.627 51 16 0 246 296 307 357 5.08e-13 69.7
MsaG042710 AT3G44460.1 39.373 287 124 13 26 294 37 291 1.11e-28 113
MsaG042710 AT3G19290.2 46.429 140 56 3 24 144 34 173 1.65e-25 105
MsaG042710 AT3G19290.4 46.429 140 56 3 24 144 34 173 2.13e-25 105
MsaG042710 AT3G19290.4 64.706 51 18 0 246 296 347 397 1.66e-13 71.2
MsaG042710 AT3G19290.3 46.429 140 56 3 24 144 34 173 2.88e-25 105
MsaG042710 AT3G19290.3 64.706 51 18 0 246 296 347 397 2.41e-13 70.9
MsaG042710 AT3G19290.1 46.429 140 56 3 24 144 34 173 2.94e-25 105
MsaG042710 AT3G19290.1 64.706 51 18 0 246 296 347 397 2.49e-13 70.9
MsaG042710 AT3G19290.5 46.429 140 56 3 24 144 34 173 2.94e-25 105
MsaG042710 AT3G19290.5 64.706 51 18 0 246 296 347 397 2.49e-13 70.9
MsaG042710 AT4G34000.3 39.130 161 58 3 24 144 27 187 8.34e-24 101
MsaG042710 AT4G34000.3 41.447 152 74 6 177 317 293 440 5.70e-16 78.6
MsaG042710 AT4G34000.1 39.130 161 58 3 24 144 27 187 1.06e-23 101
MsaG042710 AT4G34000.1 44.444 135 60 6 177 301 293 422 8.39e-16 78.2
MsaG042710 AT4G34000.4 39.130 161 58 3 24 144 27 187 1.06e-23 101
MsaG042710 AT4G34000.4 44.444 135 60 6 177 301 293 422 8.39e-16 78.2
MsaG042710 AT4G34000.2 39.130 161 58 3 24 144 27 187 1.06e-23 101
MsaG042710 AT4G34000.2 44.444 135 60 6 177 301 293 422 8.39e-16 78.2
MsaG042710 AT5G42910.1 28.763 372 167 16 23 319 19 367 5.06e-23 98.6
MsaG042710 AT1G45249.10 41.176 136 62 3 24 144 21 153 3.10e-21 93.6
MsaG042710 AT1G45249.5 41.176 136 62 3 24 144 21 153 3.10e-21 93.6
MsaG042710 AT1G45249.7 34.392 189 91 5 24 190 36 213 5.33e-21 93.2
MsaG042710 AT1G45249.7 52.632 95 33 2 236 322 341 431 3.16e-17 82.4
MsaG042710 AT1G45249.6 34.392 189 91 5 24 190 36 213 5.33e-21 93.2
MsaG042710 AT1G45249.6 52.632 95 33 2 236 322 341 431 3.16e-17 82.4
MsaG042710 AT1G45249.1 34.392 189 91 5 24 190 21 198 6.38e-21 92.8
MsaG042710 AT1G45249.1 52.632 95 33 2 236 322 326 416 2.21e-17 82.8
MsaG042710 AT1G45249.4 41.176 136 62 3 24 144 21 153 6.69e-21 92.8
MsaG042710 AT1G45249.8 41.176 136 62 3 24 144 21 153 6.69e-21 92.8
MsaG042710 AT1G45249.2 41.176 136 62 3 24 144 21 153 6.69e-21 92.8
MsaG042710 AT1G45249.9 41.176 136 62 3 24 144 21 153 6.69e-21 92.8
MsaG042710 AT1G45249.3 34.392 189 91 5 24 190 21 198 9.35e-21 92.4
MsaG042710 AT1G45249.3 47.170 106 33 3 236 322 326 427 1.65e-14 74.3
MsaG042710 AT5G44080.1 33.047 233 105 10 100 320 120 313 1.96e-18 84.7
MsaG042710 AT4G35900.1 51.136 88 38 3 236 322 202 285 1.29e-16 79.0
MsaG042710 AT2G17770.2 39.850 133 72 4 193 322 68 195 1.09e-13 69.3
MsaG042710 AT1G03970.1 30.568 229 85 8 100 310 85 257 3.05e-12 66.2

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATTGGGTGCACCTCTTA+TGG 0.223647 8:+26253893 MsaT042710.1:CDS
ATTCGACGTAGCTGATATTT+AGG 0.229724 8:-26255095 None:intergenic
GATGACATTGGAGGATTTCT+TGG 0.249290 8:+26253623 MsaT042710.1:CDS
CAAAATCAATTAGGTGACTT+AGG 0.335660 8:+26253351 MsaT042710.1:CDS
CGAGAGAGACAAACTACTTT+AGG 0.337274 8:+26253597 MsaT042710.1:CDS
CGCAGATGCCTGGAAGAAAA+AGG 0.341085 8:+26253931 MsaT042710.1:CDS
GATGAAGTTCAAAATCAATT+AGG 0.351054 8:+26253342 MsaT042710.1:CDS
AAGGATCACTCCAGAGGATA+TGG 0.352037 8:+26253950 MsaT042710.1:CDS
CTGTTTCCGTGCTCTTGAAC+GGG 0.379424 8:-26254030 None:intergenic
GCCTTCATTGTCCGTATTCG+TGG 0.383060 8:-26253442 None:intergenic
CCGTTCAAGAGCACGGAAAC+AGG 0.394263 8:+26254031 MsaT042710.1:CDS
TCTACAACGTCAAGCTAGTC+TGG 0.406297 8:+26253488 MsaT042710.1:CDS
GCATTGGGTGCACCTCTTAT+GGG 0.408513 8:+26253894 MsaT042710.1:CDS
GCAGGCTTACACAAATGAAT+TGG 0.412384 8:+26254156 MsaT042710.1:intron
CATTGTCCGTATTCGTGGAC+TGG 0.421785 8:-26253437 None:intergenic
AGAGGACTGTGGATGAGGTT+TGG 0.422682 8:+26253532 MsaT042710.1:CDS
TCTAGGTTCATACTGCTTAG+TGG 0.432596 8:-26253378 None:intergenic
TGATATTTAGGTTCAGCAAT+TGG 0.433568 8:-26255083 None:intergenic
AAAATCAATTAGGTGACTTA+GGG 0.436185 8:+26253352 MsaT042710.1:CDS
AGTATCACAGTTTCCTCTGC+AGG 0.436415 8:+26253725 MsaT042710.1:CDS
TCCGACACAGTTCCCATAAG+AGG 0.453901 8:-26253906 None:intergenic
TCCGTTTGCAGATAGTCATA+TGG 0.455759 8:+26253872 MsaT042710.1:CDS
AGAAAAGGATGATCAAGAAT+CGG 0.457820 8:+26253997 MsaT042710.1:CDS
GCAGATAGTCATATGGCATT+GGG 0.461753 8:+26253879 MsaT042710.1:CDS
CTGTTGAGAGAAGGCAGAAA+AGG 0.461801 8:+26253982 MsaT042710.1:CDS
TGCAGATAGTCATATGGCAT+TGG 0.465956 8:+26253878 MsaT042710.1:CDS
CCTGTTTCCGTGCTCTTGAA+CGG 0.468292 8:-26254031 None:intergenic
GTGTCGGATACGCAGATGCC+TGG 0.469471 8:+26253921 MsaT042710.1:CDS
GGTTCAGCAATTGGTGTACA+TGG 0.473186 8:-26255074 None:intergenic
CAGCAAGAGGACTGTGGATG+AGG 0.475392 8:+26253527 MsaT042710.1:CDS
TAAATGGGAACTCAGACAAT+GGG 0.478433 8:+26253234 None:intergenic
GAGGATTTCTTGGTGAAAGC+AGG 0.483426 8:+26253633 MsaT042710.1:CDS
GGGTCTCAAGGTGGTGGAGA+TGG 0.487387 8:+26253255 MsaT042710.1:CDS
GTCTTCTCCACCATATCCTC+TGG 0.490528 8:-26253960 None:intergenic
ATAAATGGGAACTCAGACAA+TGG 0.492628 8:+26253233 None:intergenic
TGATACTGCCACATTTGAAT+CGG 0.495093 8:-26253709 None:intergenic
TTTAGGAGAGATGACATTGG+AGG 0.495208 8:+26253614 MsaT042710.1:CDS
GGGGATTTATATGCCTAGCC+AGG 0.498570 8:+26253803 MsaT042710.1:CDS
TAAAGTTTCACGCCTGGAAG+AGG 0.502778 8:+26254183 MsaT042710.1:CDS
CAGCCGTTACACATGGGGTC+TGG 0.513878 8:+26253834 MsaT042710.1:CDS
GCCATATGACTATCTGCAAA+CGG 0.520435 8:-26253873 None:intergenic
GAAAAGGATGATCAAGAATC+GGG 0.521274 8:+26253998 MsaT042710.1:CDS
CATGGGGTCTGGTGTTTCGA+TGG 0.522204 8:+26253845 MsaT042710.1:CDS
ACTCAGACAATGGGGTCTCA+AGG 0.525496 8:+26253243 MsaT042710.1:CDS
TGTTGTAGATGCTGATATTG+TGG 0.526222 8:-26253771 None:intergenic
AGTGAACCAGTCCACGAATA+CGG 0.529125 8:+26253431 MsaT042710.1:CDS
CAAGGTGGTGGAGATGGTAG+TGG 0.530920 8:+26253261 MsaT042710.1:CDS
GCATCTACAACAAAGTTCCA+TGG 0.531471 8:+26253782 MsaT042710.1:CDS
AGTTTCCTCTGCAGGGTCAG+TGG 0.540111 8:+26253733 MsaT042710.1:CDS
CATCTACAACAAAGTTCCAT+GGG 0.551430 8:+26253783 MsaT042710.1:CDS
TAAAAGCTGCATTCAGCAAG+AGG 0.554122 8:+26253514 MsaT042710.1:CDS
GGAGATTAAAGTTTCACGCC+TGG 0.555439 8:+26254177 MsaT042710.1:CDS
AGATGCTGATATTGTGGTTG+CGG 0.557999 8:-26253765 None:intergenic
ACAATGGGGTCTCAAGGTGG+TGG 0.565619 8:+26253249 MsaT042710.1:CDS
ACCTCTTATGGGAACTGTGT+CGG 0.579301 8:+26253905 MsaT042710.1:CDS
TCCACGAATACGGACAATGA+AGG 0.580287 8:+26253441 MsaT042710.1:CDS
CTGCTGCCCGTTCAAGAGCA+CGG 0.591970 8:+26254024 MsaT042710.1:CDS
TATAGCACAGCCGTTACACA+TGG 0.595784 8:+26253827 MsaT042710.1:CDS
TGGAGAAGACTGTTGAGAGA+AGG 0.597925 8:+26253973 MsaT042710.1:CDS
GTGGAGATGGTAGTGGAAGA+CGG 0.609087 8:+26253268 MsaT042710.1:CDS
ACACCAGACCCCATGTGTAA+CGG 0.609930 8:-26253837 None:intergenic
CACAACTCCACTCTCTTGTG+AGG 0.615377 8:+26253292 MsaT042710.1:CDS
GTATCACAGTTTCCTCTGCA+GGG 0.615727 8:+26253726 MsaT042710.1:CDS
TGAAATGCTGAGGAAACGAA+AGG 0.623305 8:+26254210 MsaT042710.1:CDS
GGCTGTGCTATACCCTGGCT+AGG 0.624273 8:-26253816 None:intergenic
GAATACTGATACTACGGCTG+CGG 0.624343 8:+26253686 MsaT042710.1:CDS
GATCACTCCAGAGGATATGG+TGG 0.634046 8:+26253953 MsaT042710.1:CDS
TAGGTACACAGATCTCAGAA+TGG 0.634421 8:-26255128 None:intergenic
AAGAGGAAAATGAAATGCTG+AGG 0.635284 8:+26254200 MsaT042710.1:CDS
TACTTTAGGAGAGATGACAT+TGG 0.639124 8:+26253611 MsaT042710.1:CDS
GGCTGCGGCCGATTCAAATG+TGG 0.639880 8:+26253701 MsaT042710.1:CDS
GGGATTTATATGCCTAGCCA+GGG 0.641870 8:+26253804 MsaT042710.1:CDS
TGTAACCACTGACCCTGCAG+AGG 0.647637 8:-26253738 None:intergenic
GCTAGGCATATAAATCCCCA+TGG 0.652039 8:-26253799 None:intergenic
TAGCACAGCCGTTACACATG+GGG 0.656286 8:+26253829 MsaT042710.1:CDS
CAGACAATGGGGTCTCAAGG+TGG 0.688721 8:+26253246 MsaT042710.1:CDS
GTAACGGCTGTGCTATACCC+TGG 0.689448 8:-26253821 None:intergenic
ATAGCACAGCCGTTACACAT+GGG 0.689708 8:+26253828 MsaT042710.1:CDS
TGCATTCAGCAAGAGGACTG+TGG 0.691129 8:+26253521 MsaT042710.1:CDS
AAATGGGAACTCAGACAATG+GGG 0.712282 8:+26253235 None:intergenic
ATCTACAACAAAGTTCCATG+GGG 0.739377 8:+26253784 MsaT042710.1:CDS
CCAACAAAAGAAAGACAACG+AGG 0.748926 8:+26253563 MsaT042710.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 26253237 26255136 26253237 ID=MsaG042710
Chr8 mRNA 26253237 26255136 26253237 ID=MsaT042710.1;Parent=MsaG042710
Chr8 exon 26253237 26254052 26253237 ID=MsaT042710.1.exon1;Parent=MsaT042710.1
Chr8 CDS 26253237 26254052 26253237 ID=cds.MsaT042710.1;Parent=MsaT042710.1
Chr8 exon 26254160 26254231 26254160 ID=MsaT042710.1.exon2;Parent=MsaT042710.1
Chr8 CDS 26254160 26254231 26254160 ID=cds.MsaT042710.1;Parent=MsaT042710.1
Chr8 exon 26255056 26255136 26255056 ID=MsaT042710.1.exon3;Parent=MsaT042710.1
Chr8 CDS 26255056 26255136 26255056 ID=cds.MsaT042710.1;Parent=MsaT042710.1
Gene Sequence

>MsaT042710.1

ATGGGAACTCAGACAATGGGGTCTCAAGGTGGTGGAGATGGTAGTGGAAGACGGTCACAACTCCACTCTCTTGTGAGGCAAAATTCAGTGTACAGTCTCACACTAGATGAAGTTCAAAATCAATTAGGTGACTTAGGGAAGCCACTAAGCAGTATGAACCTAGACGAGCTTCTGAAAAATGTATGGACTGTTGAAGTGAACCAGTCCACGAATACGGACAATGAAGGCACAACGCAATCTAGTGAAGCTTCTCTACAACGTCAAGCTAGTCTGGCATTAAAAGCTGCATTCAGCAAGAGGACTGTGGATGAGGTTTGGAGAGATATCCAACAAAAGAAAGACAACGAGGAAAAGAAGTCACGAGAGAGACAAACTACTTTAGGAGAGATGACATTGGAGGATTTCTTGGTGAAAGCAGGTATTGTTGCAGAAGCATCTTCTAATAAAAAGAATACTGATACTACGGCTGCGGCCGATTCAAATGTGGCAGTATCACAGTTTCCTCTGCAGGGTCAGTGGTTACAGTATCCGCAACCACAATATCAGCATCTACAACAAAGTTCCATGGGGATTTATATGCCTAGCCAGGGTATAGCACAGCCGTTACACATGGGGTCTGGTGTTTCGATGGAGATTCCGTTTGCAGATAGTCATATGGCATTGGGTGCACCTCTTATGGGAACTGTGTCGGATACGCAGATGCCTGGAAGAAAAAGGATCACTCCAGAGGATATGGTGGAGAAGACTGTTGAGAGAAGGCAGAAAAGGATGATCAAGAATCGGGAATCTGCTGCCCGTTCAAGAGCACGGAAACAGGCTTACACAAATGAATTGGAGATTAAAGTTTCACGCCTGGAAGAGGAAAATGAAATGCTGAGGAAACGAAAGGAGCTAGAGAGTATGTTGCCATGTACACCAATTGCTGAACCTAAATATCAGCTACGTCGAATAGCATCTTGTCCATTCTGA

Protein sequence

>MsaT042710.1

MGTQTMGSQGGGDGSGRRSQLHSLVRQNSVYSLTLDEVQNQLGDLGKPLSSMNLDELLKNVWTVEVNQSTNTDNEGTTQSSEASLQRQASLALKAAFSKRTVDEVWRDIQQKKDNEEKKSRERQTTLGEMTLEDFLVKAGIVAEASSNKKNTDTTAAADSNVAVSQFPLQGQWLQYPQPQYQHLQQSSMGIYMPSQGIAQPLHMGSGVSMEIPFADSHMALGAPLMGTVSDTQMPGRKRITPEDMVEKTVERRQKRMIKNRESAARSRARKQAYTNELEIKVSRLEEENEMLRKRKELESMLPCTPIAEPKYQLRRIASCPF*