AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042818

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042818 MtrunA17_Chr8g0356241 94.391 517 8 4 1 501 1 512 0.0 991
MsaG042818 MtrunA17_Chr5g0438891 33.903 351 153 12 143 465 36 335 5.88e-49 173
MsaG042818 MtrunA17_Chr5g0438891 42.169 83 40 2 144 226 263 337 8.24e-11 63.9
MsaG042818 MtrunA17_Chr4g0058011 32.164 342 122 11 195 465 231 533 2.09e-43 162
MsaG042818 MtrunA17_Chr4g0058011 35.088 171 68 4 300 470 231 358 5.60e-25 108
MsaG042818 MtrunA17_Chr4g0058011 32.624 141 63 4 143 260 231 362 1.58e-16 82.4
MsaG042818 MtrunA17_Chr4g0058011 40.244 82 30 2 190 252 453 534 7.93e-12 67.8
MsaG042818 MtrunA17_Chr7g0249221 30.789 393 181 15 116 465 6 350 8.02e-41 151
MsaG042818 MtrunA17_Chr7g0249221 33.163 196 86 3 276 471 3 153 2.73e-27 114
MsaG042818 MtrunA17_Chr3g0116431 31.515 330 150 10 228 485 177 502 4.30e-38 145
MsaG042818 MtrunA17_Chr3g0116431 27.273 352 134 9 143 417 171 477 1.38e-28 118
MsaG042818 MtrunA17_Chr3g0116431 34.010 197 84 6 301 497 172 322 1.12e-24 107
MsaG042818 MtrunA17_Chr3g0116431 41.667 84 30 2 190 254 395 478 3.27e-12 68.6
MsaG042818 MtrunA17_Chr1g0195891 30.149 335 183 11 136 444 39 348 4.88e-36 137
MsaG042818 MtrunA17_Chr1g0195891 34.132 167 65 3 301 467 47 168 7.53e-22 96.7
MsaG042818 MtrunA17_Chr1g0195891 45.000 80 39 1 386 465 46 120 4.91e-15 76.6
MsaG042818 MtrunA17_Chr2g0286151 38.012 171 63 4 301 471 45 172 3.72e-27 114
MsaG042818 MtrunA17_Chr2g0286151 28.406 345 167 12 143 416 44 379 3.76e-25 108
MsaG042818 MtrunA17_Chr2g0286151 42.500 80 42 1 386 465 44 119 7.33e-19 89.4
MsaG042818 MtrunA17_Chr2g0286151 40.000 80 44 1 386 465 303 378 5.77e-11 64.7
MsaG042818 MtrunA17_Chr4g0066941 36.723 177 65 4 301 476 44 174 3.50e-25 108
MsaG042818 MtrunA17_Chr4g0066941 33.974 156 53 3 193 330 41 164 1.22e-22 100
MsaG042818 MtrunA17_Chr4g0066941 35.556 135 61 3 143 258 43 170 3.36e-21 96.7
MsaG042818 MtrunA17_Chr4g0066941 49.367 79 34 1 387 465 333 405 3.81e-18 87.4
MsaG042818 MtrunA17_Chr4g0066941 41.250 80 42 1 386 465 43 117 6.18e-17 83.6
MsaG042818 MtrunA17_Chr4g0066941 42.683 82 39 3 144 225 333 406 2.54e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042818 AT3G12680.1 57.488 414 137 10 100 488 125 524 2.10e-168 486
MsaG042818 AT5G16540.3 33.758 314 130 6 194 465 17 294 7.48e-55 188
MsaG042818 AT5G16540.3 32.209 326 121 9 143 416 18 295 4.17e-39 145
MsaG042818 AT5G16540.3 30.890 191 87 4 301 491 19 164 1.68e-25 107
MsaG042818 AT5G16540.3 44.828 87 42 2 379 465 12 92 2.90e-17 83.6
MsaG042818 AT5G16540.3 44.706 85 37 2 142 224 218 294 1.84e-14 75.1
MsaG042818 AT5G16540.1 33.758 314 130 6 194 465 38 315 8.70e-55 188
MsaG042818 AT5G16540.1 32.209 326 121 9 143 416 39 316 4.79e-39 146
MsaG042818 AT5G16540.1 30.890 191 87 4 301 491 40 185 1.49e-25 108
MsaG042818 AT5G16540.1 33.088 136 67 3 330 465 2 113 2.57e-17 84.0
MsaG042818 AT5G16540.1 44.706 85 37 2 142 224 239 315 1.86e-14 75.5
MsaG042818 AT2G47850.1 32.033 359 123 11 192 465 39 361 1.89e-50 179
MsaG042818 AT2G47850.1 38.636 176 61 3 293 467 35 164 4.97e-28 117
MsaG042818 AT2G47850.1 44.330 97 49 2 369 465 25 116 9.13e-21 95.5
MsaG042818 AT2G47850.1 42.683 82 28 2 189 251 280 361 2.22e-13 72.8
MsaG042818 AT2G47850.3 32.033 359 123 11 192 465 39 361 1.89e-50 179
MsaG042818 AT2G47850.3 38.636 176 61 3 293 467 35 164 4.97e-28 117
MsaG042818 AT2G47850.3 44.330 97 49 2 369 465 25 116 9.13e-21 95.5
MsaG042818 AT2G47850.3 42.683 82 28 2 189 251 280 361 2.22e-13 72.8
MsaG042818 AT3G02830.2 31.844 358 128 11 188 465 5 326 6.56e-50 175
MsaG042818 AT3G02830.2 32.821 195 86 3 287 481 4 153 2.83e-28 116
MsaG042818 AT3G02830.2 29.032 341 154 11 143 442 62 355 3.57e-28 115
MsaG042818 AT3G02830.2 44.706 85 37 2 142 224 250 326 2.85e-14 74.7
MsaG042818 AT3G02830.2 34.848 132 49 5 190 284 245 376 2.57e-12 68.9
MsaG042818 AT5G16540.2 32.484 314 127 7 194 465 38 308 1.42e-49 174
MsaG042818 AT5G16540.2 30.982 326 118 9 143 416 39 309 6.88e-35 134
MsaG042818 AT5G16540.2 30.890 191 87 4 301 491 40 185 1.50e-25 108
MsaG042818 AT5G16540.2 33.088 136 67 3 330 465 2 113 2.33e-17 84.3
MsaG042818 AT3G02830.1 31.926 379 192 12 111 465 10 346 3.78e-46 166
MsaG042818 AT3G02830.1 32.500 200 90 3 282 481 19 173 1.61e-28 117
MsaG042818 AT3G02830.1 34.848 132 49 5 190 284 265 396 2.59e-12 68.9
MsaG042818 AT5G16540.4 32.292 288 117 6 194 439 17 268 1.10e-43 155
MsaG042818 AT5G16540.4 33.865 251 106 7 143 341 18 260 3.24e-32 124
MsaG042818 AT5G16540.4 30.890 191 87 4 301 491 19 164 8.57e-26 107
MsaG042818 AT5G16540.4 44.828 87 42 2 379 465 12 92 1.92e-17 82.8
MsaG042818 AT5G63260.2 27.484 473 211 13 62 467 17 424 3.67e-43 159
MsaG042818 AT2G47850.2 32.565 347 157 13 143 465 42 335 4.93e-41 153
MsaG042818 AT2G47850.2 44.330 97 49 2 369 465 25 116 6.07e-21 95.9
MsaG042818 AT3G48440.1 32.362 309 133 8 228 464 113 417 2.43e-40 151
MsaG042818 AT3G48440.1 37.126 167 63 3 301 467 108 232 1.27e-26 112
MsaG042818 AT5G63260.1 30.968 310 139 7 228 467 104 408 4.83e-38 144
MsaG042818 AT5G63260.1 29.048 210 98 7 62 225 17 221 9.50e-15 76.6
MsaG042818 AT5G63260.1 29.457 129 48 2 385 470 97 225 3.73e-14 75.1
MsaG042818 AT5G18550.1 30.194 361 175 17 143 464 48 370 6.02e-38 145
MsaG042818 AT5G18550.1 27.273 341 155 11 193 485 46 341 7.16e-32 128
MsaG042818 AT5G18550.3 30.194 361 175 17 143 464 16 338 6.50e-38 144
MsaG042818 AT5G18550.3 35.366 164 57 4 193 337 14 147 1.22e-28 118
MsaG042818 AT5G18550.3 34.118 170 69 4 300 468 16 143 1.16e-23 103
MsaG042818 AT5G18550.4 30.194 361 175 17 143 464 48 370 7.28e-38 144
MsaG042818 AT5G18550.4 27.273 341 155 11 193 485 46 341 7.96e-32 127
MsaG042818 AT1G04990.1 29.878 328 133 9 196 465 45 333 1.57e-36 140
MsaG042818 AT1G04990.1 37.725 167 56 3 301 467 45 163 4.65e-28 116
MsaG042818 AT1G04990.1 40.876 137 53 5 135 252 35 162 4.26e-25 107
MsaG042818 AT1G04990.1 39.241 79 42 1 149 227 264 336 3.02e-11 65.9
MsaG042818 AT1G04990.6 29.878 328 133 9 196 465 45 333 1.57e-36 140
MsaG042818 AT1G04990.6 37.725 167 56 3 301 467 45 163 4.65e-28 116
MsaG042818 AT1G04990.6 40.876 137 53 5 135 252 35 162 4.26e-25 107
MsaG042818 AT1G04990.6 39.241 79 42 1 149 227 264 336 3.02e-11 65.9
MsaG042818 AT1G04990.2 29.878 328 133 9 196 465 45 333 1.57e-36 140
MsaG042818 AT1G04990.2 37.725 167 56 3 301 467 45 163 4.65e-28 116
MsaG042818 AT1G04990.2 40.876 137 53 5 135 252 35 162 4.26e-25 107
MsaG042818 AT1G04990.2 39.241 79 42 1 149 227 264 336 3.02e-11 65.9
MsaG042818 AT2G32930.3 30.792 341 172 9 143 464 14 309 2.82e-36 139
MsaG042818 AT2G32930.3 37.126 167 58 4 301 467 15 134 3.04e-28 117
MsaG042818 AT2G32930.3 30.363 303 141 12 188 465 7 264 7.00e-27 113
MsaG042818 AT2G32930.2 30.792 341 172 9 143 464 41 336 2.87e-36 140
MsaG042818 AT2G32930.2 35.000 200 83 4 268 467 9 161 5.91e-29 119
MsaG042818 AT2G32930.2 30.363 303 141 12 188 465 34 291 5.82e-27 114
MsaG042818 AT5G18550.2 29.086 361 166 17 143 464 48 357 1.06e-33 133
MsaG042818 AT5G18550.2 28.060 335 148 11 193 479 46 335 1.14e-32 130
MsaG042818 AT1G04990.5 28.354 328 118 9 196 465 45 313 3.35e-33 130
MsaG042818 AT1G04990.5 37.725 167 56 3 301 467 45 163 3.67e-28 116
MsaG042818 AT1G04990.5 40.876 137 53 5 135 252 35 162 3.45e-25 107
MsaG042818 AT2G32930.1 29.912 341 171 10 143 464 41 332 5.97e-32 128
MsaG042818 AT2G32930.1 34.135 208 84 6 262 467 1 157 3.78e-25 108
MsaG042818 AT2G32930.1 29.766 299 144 11 188 465 34 287 1.17e-24 107
MsaG042818 AT2G47850.4 32.510 243 90 8 293 465 1 239 5.35e-30 120
MsaG042818 AT2G47850.4 45.349 86 33 3 143 224 164 239 3.92e-14 74.3
MsaG042818 AT2G47850.4 43.590 78 25 2 193 251 162 239 8.21e-14 73.2
MsaG042818 AT1G04990.3 29.710 276 113 8 232 465 16 252 5.37e-26 108
MsaG042818 AT1G04990.3 47.368 76 35 1 392 467 12 82 2.11e-17 83.6
MsaG042818 AT1G04990.3 39.241 79 42 1 149 227 183 255 1.24e-11 66.2
MsaG042818 AT3G06410.1 33.540 161 58 4 193 334 49 179 7.74e-25 107
MsaG042818 AT3G06410.1 35.714 168 64 5 301 467 52 176 1.39e-23 103
MsaG042818 AT3G06410.1 35.766 137 57 4 143 256 51 179 6.98e-20 92.8
MsaG042818 AT3G06410.1 38.525 122 65 3 343 464 267 378 5.58e-19 90.1
MsaG042818 AT3G06410.1 41.250 80 43 1 386 465 51 126 6.57e-18 86.7
MsaG042818 AT3G06410.1 30.000 170 84 6 59 223 239 378 1.83e-12 69.7
MsaG042818 AT1G04990.4 27.174 276 100 7 232 465 16 232 1.60e-22 98.2
MsaG042818 AT1G04990.4 47.368 76 35 1 392 467 12 82 2.26e-17 83.2
MsaG042818 AT1G48195.1 44.304 79 40 1 386 464 6 80 3.68e-17 76.6

Find 156 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTGGCATATGTAAATT+TGG 0.142400 8:-28035237 MsaT042818.1:CDS
GCAAGTTCGATCATCCTATT+TGG 0.183299 8:-28038667 MsaT042818.1:CDS
GTTTCACTTGGAACAATATT+TGG 0.192357 8:+28037270 None:intergenic
GGCGACGGCGGCAGCGGCGG+AGG 0.196569 8:+28040579 None:intergenic
CTAACAAGAACCTGTAAATT+TGG 0.229682 8:-28038698 MsaT042818.1:CDS
TTGCCCAATTGTACTCAAAA+TGG 0.244916 8:+28038835 None:intergenic
CAGTCTCAGGTGGGAGTAAC+AGG 0.258392 8:-28035701 MsaT042818.1:CDS
GTACTTCCGGTTTCATATCT+AGG 0.258578 8:+28038773 None:intergenic
GCAGCTCTCTCCAAATTTAC+AGG 0.264926 8:+28038688 None:intergenic
TGCATTGGGCCCTTCTGTTT+TGG 0.269913 8:-28036139 MsaT042818.1:CDS
AAAACTGCAACGTGCAAGTT+TGG 0.272112 8:-28034731 MsaT042818.1:CDS
AACAAACATACCTCTCTTCT+TGG 0.273988 8:+28035126 None:intergenic
GGAAGCAGCGGCGGCGGCTT+CGG 0.278346 8:+28040690 None:intergenic
CCTGGCCTCTGAGGATAAAT+AGG 0.293250 8:+28038746 None:intergenic
AATCTGGCATATGTAAATTT+GGG 0.314408 8:-28035236 MsaT042818.1:CDS
TGCAGACACAGGTGGATTAA+TGG 0.315809 8:+28036158 None:intergenic
ATGTAGTTCTACTTGAAAAC+TGG 0.326854 8:-28036761 MsaT042818.1:intron
AGCAGCGGCGACGGCGGCAG+CGG 0.330670 8:+28040573 None:intergenic
CCACCCGCAGGAACCCAAAT+AGG 0.335891 8:+28038653 None:intergenic
AGCGGCGACGGCGGCAGCGG+CGG 0.341849 8:+28040576 None:intergenic
TTTATTCGGGTCGTAATATT+GGG 0.346440 8:+28040741 None:intergenic
ACAAATTCTTCTGTGTTTGA+TGG 0.349596 8:-28036641 MsaT042818.1:CDS
TCTGTGTTTGATGGGGCAAT+AGG 0.371334 8:-28036632 MsaT042818.1:CDS
CAAGTTCGATCATCCTATTT+GGG 0.377080 8:-28038666 MsaT042818.1:CDS
TGTCCATTCTACATGAAAAC+TGG 0.383033 8:-28036411 MsaT042818.1:CDS
ATGCAGTTCTATATGAAATC+TGG 0.398754 8:-28035252 MsaT042818.1:intron
CAAATTCTTCTGTGTTTGAT+GGG 0.399035 8:-28036640 MsaT042818.1:CDS
AATCCACCTGTGTCTGCATT+GGG 0.400528 8:-28036153 MsaT042818.1:CDS
AATGTTAAACTTACTGCTGC+AGG 0.401933 8:-28035153 MsaT042818.1:CDS
AGGTTCTTGTTAGCATATAG+TGG 0.401971 8:+28038708 None:intergenic
AATCATCAGATAATTGAATC+TGG 0.404625 8:+28036690 None:intergenic
TAATCCACCTGTGTCTGCAT+TGG 0.404899 8:-28036154 MsaT042818.1:CDS
GCGACGGCGGCAGCGGCGGA+GGG 0.408489 8:+28040580 None:intergenic
ATAAATAGGCAAAGTACTTC+CGG 0.408705 8:+28038760 None:intergenic
TCGGGTCGTAATATTGGGTC+GGG 0.411798 8:+28040746 None:intergenic
AATTGAATCTGGATATCCTT+GGG 0.412002 8:+28036701 None:intergenic
AGCCATCTCCATCACTTCTC+CGG 0.413222 8:+28034679 None:intergenic
GGTCTCCGTTTCTCCTCCAT+TGG 0.417479 8:+28034634 None:intergenic
ATAGTCACCTCTTTCCAATC+TGG 0.419919 8:+28038626 None:intergenic
AGTGATGTTGTGCGGTCGAT+TGG 0.423384 8:+28035192 None:intergenic
ATCTGGTTCTCCAGGTCTCT+CGG 0.423563 8:+28037242 None:intergenic
TTTGTGTCTGCTCAACATTA+CGG 0.425536 8:+28036663 None:intergenic
AGGATGATTGTAGCGGCAAC+TGG 0.426982 8:+28036268 None:intergenic
AAGAGGATGTGTTGTAGATT+CGG 0.436282 8:+28040806 None:intergenic
TGGGTCGGGGATGTTGTTGA+TGG 0.438909 8:+28040760 None:intergenic
AGCAGCGGCGGCGGCTTCGG+CGG 0.439320 8:+28040693 None:intergenic
GATGGAGGAAGCAGCGGCGG+CGG 0.441417 8:+28040684 None:intergenic
GCATGATCTCAATATTGTTC+CGG 0.445718 8:+28034587 None:intergenic
CGACGGCGGCAGCGGCGGAG+GGG 0.446303 8:+28040581 None:intergenic
GATTTCGACATTGCTCCTCC+TGG 0.447120 8:-28040649 MsaT042818.1:CDS
GAATCTCCACCACTAATCCT+TGG 0.448603 8:-28040615 MsaT042818.1:CDS
ATATAAATCAGTTGCAAGTT+CGG 0.454222 8:-28036293 MsaT042818.1:intron
GTTCTACTCACTGGACAATC+TGG 0.456349 8:+28037225 None:intergenic
TCCATCACTTCTCCGGGTGG+TGG 0.461867 8:+28034686 None:intergenic
CTCTCGGGAAGAGTTTCACT+TGG 0.463041 8:+28037258 None:intergenic
TCCTCTTACAACATCCCAAC+CGG 0.466266 8:-28040790 MsaT042818.1:CDS
AGGAGGAGCAATGTCGAAAT+CGG 0.468141 8:+28040651 None:intergenic
TACCATTGGATTTGACAGAC+TGG 0.473228 8:+28036047 None:intergenic
CATCCTATTTGGGTTCCTGC+GGG 0.475897 8:-28038656 MsaT042818.1:CDS
CGGCGGCGGCTTCGGCGGTG+CGG 0.478211 8:+28040698 None:intergenic
TAATTGAATCTGGATATCCT+TGG 0.480053 8:+28036700 None:intergenic
TAGTCACCTCTTTCCAATCT+GGG 0.484064 8:+28038627 None:intergenic
TCTGGTTCTCCAGGTCTCTC+GGG 0.484418 8:+28037243 None:intergenic
AAAACTGGAAAATGTAAATA+CGG 0.484664 8:-28036746 MsaT042818.1:CDS
TCCTCAGAGGCCAGGAGAGA+AGG 0.488900 8:-28038738 MsaT042818.1:CDS
TACTTCCGGTTTCATATCTA+GGG 0.492109 8:+28038774 None:intergenic
TTCGGGTCGTAATATTGGGT+CGG 0.492124 8:+28040745 None:intergenic
TGTTGTTGATGGAGCTGCTC+CGG 0.493176 8:+28040771 None:intergenic
TCTGGATATCCTTGGGGTGA+TGG 0.494056 8:+28036708 None:intergenic
TGGGCACAATCCTTCTCTCC+TGG 0.495065 8:+28038728 None:intergenic
ACATCACATTCAATCTGTCC+AGG 0.499616 8:+28035659 None:intergenic
CACTGTCGCATAATAGCAAA+GGG 0.501308 8:-28036577 MsaT042818.1:CDS
CTGCGGGTGGAATCCCAGAT+TGG 0.502089 8:-28038640 MsaT042818.1:CDS
ACTATCTATCCTCAGCGCCC+TGG 0.504341 8:-28035677 MsaT042818.1:CDS
CCTATTTATCCTCAGAGGCC+AGG 0.504717 8:-28038746 MsaT042818.1:CDS
AATCTCCACCACTAATCCTT+GGG 0.505009 8:-28040614 MsaT042818.1:CDS
TTGATGGAGCTGCTCCGGTT+GGG 0.506701 8:+28040776 None:intergenic
AGCATTCGCCCAAGGATTAG+TGG 0.507557 8:+28040606 None:intergenic
AGATGTGCCTGGTTCTGCTC+CGG 0.508060 8:-28034606 MsaT042818.1:CDS
GCCATCTCCATCACTTCTCC+GGG 0.508482 8:+28034680 None:intergenic
ATTTGTTGCAGCAGCGGCGA+CGG 0.508564 8:+28040564 None:intergenic
TACAACCAATGGAGGAGAAA+CGG 0.511068 8:-28034639 MsaT042818.1:CDS
CAAAGGGCTTCCTGTGAGAC+CGG 0.513415 8:-28036561 MsaT042818.1:intron
AAAATTAATACCGGTCTCAC+AGG 0.514093 8:+28036551 None:intergenic
ACTGGACAATCTGGTTCTCC+AGG 0.515598 8:+28037234 None:intergenic
GTGATGTTGTGCGGTCGATT+GGG 0.517527 8:+28035193 None:intergenic
TCACTTCTCCGGGTGGTGGA+TGG 0.518278 8:+28034690 None:intergenic
GAAACTCTTCCCGAGAGACC+TGG 0.522394 8:-28037252 MsaT042818.1:CDS
TGTTGCAGCAGCGGCGACGG+CGG 0.524901 8:+28040567 None:intergenic
GAGATTCTAGACGCAACTCC+AGG 0.526633 8:+28040631 None:intergenic
GTTGATGGAGCTGCTCCGGT+TGG 0.526797 8:+28040775 None:intergenic
TACAATCATCCTGACATGAA+TGG 0.530255 8:-28036257 MsaT042818.1:intron
GGCGACATTTGTTGCAGCAG+CGG 0.532990 8:+28040558 None:intergenic
CCTATTTGGGTTCCTGCGGG+TGG 0.532997 8:-28038653 MsaT042818.1:CDS
AAGCGCCCTAGATATGAAAC+CGG 0.536130 8:-28038779 MsaT042818.1:CDS
GGTTTCATATCTAGGGCGCT+TGG 0.536573 8:+28038781 None:intergenic
ACCATTGGATTTGACAGACT+GGG 0.537056 8:+28036048 None:intergenic
CAATTGGGCAAAACGAAGCT+TGG 0.537177 8:-28038823 MsaT042818.1:CDS
TTGGTATACGTCGAACTCAC+TGG 0.538046 8:-28038804 MsaT042818.1:CDS
TATTGTTCCGGAGCAGAACC+AGG 0.544228 8:+28034599 None:intergenic
GATCTTAAACGCTCTTCTGA+TGG 0.548162 8:-28040520 MsaT042818.1:intron
TTGTGCGGTCGATTGGGTGA+TGG 0.553158 8:+28035199 None:intergenic
TCCACCACCCGGAGAAGTGA+TGG 0.555622 8:-28034687 MsaT042818.1:CDS
TTATACATACCATTCATGTC+AGG 0.559421 8:+28036248 None:intergenic
TCATCCTATTTGGGTTCCTG+CGG 0.562825 8:-28038657 MsaT042818.1:CDS
TGCGACAGTGGAGATATAAG+AGG 0.563819 8:+28036590 None:intergenic
GTTGAGGACGCCAAAACAGA+AGG 0.568271 8:+28036129 None:intergenic
TTGTACTCAAAATGGATGGA+TGG 0.570115 8:+28038843 None:intergenic
CAAGGAGCTTCTACAACCAA+TGG 0.571919 8:-28034650 MsaT042818.1:CDS
CCACTGTCGCATAATAGCAA+AGG 0.572283 8:-28036578 MsaT042818.1:CDS
TGTCGAAATCGGAGGAGTAG+CGG 0.577021 8:+28040662 None:intergenic
GGTTCTTGTTAGCATATAGT+GGG 0.577333 8:+28038709 None:intergenic
AAATTCTTCTGTGTTTGATG+GGG 0.579820 8:-28036639 MsaT042818.1:CDS
GAGACCAACGAAGATGTGCC+TGG 0.580549 8:-28034617 MsaT042818.1:CDS
TCAACTGCTAATCTTAACAT+TGG 0.585935 8:-28036111 MsaT042818.1:CDS
GAGGGGAAAGCATTCGCCCA+AGG 0.587222 8:+28040598 None:intergenic
AGAACCAGGCACATCTTCGT+TGG 0.587281 8:+28034613 None:intergenic
ATCGGAGGAGTAGCGGATGG+AGG 0.587976 8:+28040669 None:intergenic
ATGTTAAACTTACTGCTGCA+GGG 0.593446 8:-28035152 MsaT042818.1:CDS
TCCGGTTGGGATGTTGTAAG+AGG 0.594805 8:+28040789 None:intergenic
ATCTGGCATATGTAAATTTG+GGG 0.597625 8:-28035235 MsaT042818.1:CDS
TCCTTCTCTCCTGGCCTCTG+AGG 0.599748 8:+28038737 None:intergenic
CCCCAGTCTGTCAAATCCAA+TGG 0.601676 8:-28036049 MsaT042818.1:intron
GCGGATGGAGGAAGCAGCGG+CGG 0.603426 8:+28040681 None:intergenic
CAATCTGGGATTCCACCCGC+AGG 0.605325 8:+28038641 None:intergenic
GTAGCGGATGGAGGAAGCAG+CGG 0.608163 8:+28040678 None:intergenic
CTTTGCCTATTTATCCTCAG+AGG 0.609724 8:-28038751 MsaT042818.1:CDS
CATTCTACATGAAAACTGGA+AGG 0.612306 8:-28036407 MsaT042818.1:intron
AAATTTGACCATCCACCACC+CGG 0.612670 8:-28034698 MsaT042818.1:CDS
CGGTGCGGCGATAGTGAGCG+AGG 0.620003 8:+28040713 None:intergenic
CCAATTGTACTCAAAATGGA+TGG 0.622960 8:+28038839 None:intergenic
ATCTCCATCACTTCTCCGGG+TGG 0.623307 8:+28034683 None:intergenic
GGGCCCAATGCAGACACAGG+TGG 0.623860 8:+28036150 None:intergenic
TGGAATCCCAGATTGGAAAG+AGG 0.624036 8:-28038633 MsaT042818.1:intron
GAAATCGGAGGAGTAGCGGA+TGG 0.628796 8:+28040666 None:intergenic
TTGAGGACGCCAAAACAGAA+GGG 0.632080 8:+28036130 None:intergenic
GAAGGGCCCAATGCAGACAC+AGG 0.635952 8:+28036147 None:intergenic
AATGTTAAGATTAGCAGTTG+AGG 0.638653 8:+28036113 None:intergenic
ATGGAGATGGCTAAGTCACA+AGG 0.639872 8:-28034668 MsaT042818.1:CDS
ACCCGGAGAAGTGATGGAGA+TGG 0.642505 8:-28034681 MsaT042818.1:CDS
CATCACATTCAATCTGTCCA+GGG 0.642800 8:+28035660 None:intergenic
CCATTGGATTTGACAGACTG+GGG 0.644721 8:+28036049 None:intergenic
TCAATCTGTCCAGGGCGCTG+AGG 0.644841 8:+28035668 None:intergenic
CGGGTCGTAATATTGGGTCG+GGG 0.646912 8:+28040747 None:intergenic
AGGAGCAATGTCGAAATCGG+AGG 0.653515 8:+28040654 None:intergenic
AGTCTCAGGTGGGAGTAACA+GGG 0.654906 8:-28035700 MsaT042818.1:CDS
TGCAGGGTTACCAAGAAGAG+AGG 0.662159 8:-28035136 MsaT042818.1:intron
ATTGAATCTGGATATCCTTG+GGG 0.664522 8:+28036702 None:intergenic
AAGATCAAGTGAAGCCGCAG+AGG 0.666582 8:+28040537 None:intergenic
TGCAACAAATGTCGCCTCTG+CGG 0.667972 8:-28040551 MsaT042818.1:CDS
GGAGCTTCTACAACCAATGG+AGG 0.668592 8:-28034647 MsaT042818.1:CDS
TCATGTCAGGATGATTGTAG+CGG 0.686151 8:+28036261 None:intergenic
ATGCAAATTCCATCACCCCA+AGG 0.686683 8:-28036717 MsaT042818.1:CDS
ATTCTAGACGCAACTCCAGG+AGG 0.695785 8:+28040634 None:intergenic
ATTCGCCCAAGGATTAGTGG+TGG 0.702300 8:+28040609 None:intergenic
CCTTTGCTATTATGCGACAG+TGG 0.707348 8:+28036578 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 28034595 28040836 28034595 ID=MsaG042818
Chr8 mRNA 28034595 28040836 28034595 ID=MsaT042818.1;Parent=MsaG042818
Chr8 exon 28034595 28034780 28034595 ID=MsaT042818.1.exon11;Parent=MsaT042818.1
Chr8 CDS 28034595 28034780 28034595 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28035137 28035268 28035137 ID=MsaT042818.1.exon10;Parent=MsaT042818.1
Chr8 CDS 28035137 28035268 28035137 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28035661 28035720 28035661 ID=MsaT042818.1.exon9;Parent=MsaT042818.1
Chr8 CDS 28035661 28035720 28035661 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28036050 28036180 28036050 ID=MsaT042818.1.exon8;Parent=MsaT042818.1
Chr8 CDS 28036050 28036180 28036050 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28036258 28036304 28036258 ID=MsaT042818.1.exon7;Parent=MsaT042818.1
Chr8 CDS 28036258 28036304 28036258 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28036408 28036445 28036408 ID=MsaT042818.1.exon6;Parent=MsaT042818.1
Chr8 CDS 28036408 28036445 28036408 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28036562 28036777 28036562 ID=MsaT042818.1.exon5;Parent=MsaT042818.1
Chr8 CDS 28036562 28036777 28036562 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28037061 28037141 28037061 ID=MsaT042818.1.exon4;Parent=MsaT042818.1
Chr8 CDS 28037061 28037141 28037061 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28037236 28037295 28037236 ID=MsaT042818.1.exon3;Parent=MsaT042818.1
Chr8 CDS 28037236 28037295 28037236 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28038634 28038872 28038634 ID=MsaT042818.1.exon2;Parent=MsaT042818.1
Chr8 CDS 28038634 28038872 28038634 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Chr8 exon 28040521 28040836 28040521 ID=MsaT042818.1.exon1;Parent=MsaT042818.1
Chr8 CDS 28040521 28040836 28040521 ID=cds.MsaT042818.1;Parent=MsaT042818.1
Gene Sequence

>MsaT042818.1

ATGGAAAACCGAATCTACAACACATCCTCTTACAACATCCCAACCGGAGCAGCTCCATCAACAACATCCCCGACCCAATATTACGACCCGAATAAAATGTTCCTCGCTCACTATCGCCGCACCGCCGAAGCCGCCGCCGCTGCTTCCTCCATCCGCTACTCCTCCGATTTCGACATTGCTCCTCCTGGAGTTGCGTCTAGAATCTCCACCACTAATCCTTGGGCGAATGCTTTCCCCTCCGCCGCTGCCGCCGTCGCCGCTGCTGCAACAAATGTCGCCTCTGCGGCTTCACTTGATCTTAAACGCTCTTCTGATGCGCTCTACCATCCATCCATTTTGAGTACAATTGGGCAAAACGAAGCTTGGTATACGTCGAACTCACTGGCCAAGCGCCCTAGATATGAAACCGGAAGTACTTTGCCTATTTATCCTCAGAGGCCAGGAGAGAAGGATTGTGCCCACTATATGCTAACAAGAACCTGTAAATTTGGAGAGAGCTGCAAGTTCGATCATCCTATTTGGGTTCCTGCGGGTGGAATCCCAGATTGGAAAGAGGTTCCAAATATTGTTCCAAGTGAAACTCTTCCCGAGAGACCTGGAGAACCAGATTGTCCATTTTTTCTGAAAACTCAAAAATGCAAGTTTGGTACAAAGTGCAAATTTAATCATCCAAATGTTCCCTCTGAAAATGCCGATTTCTACTTGAAAACTGGAAAATGTAAATACGGTGCTGCATGCAAATTCCATCACCCCAAGGATATCCAGATTCAATTATCTGATGATTTGAGCCGTAATGTTGAGCAGACACAAACAAATTCTTCTGTGTTTGATGGGGCAATAGGAGATACACAGTCAACTATGCCTCTTATATCTCCACTGTCGCATAATAGCAAAGGGCTTCCTGTGAGACCGGGGGAAGTGGATTGTCCATTCTACATGAAAACTGGAAGTTGCAAGTTCGGTGCCAGTTGCCGCTACAATCATCCTGACATGAATGCCATTAATCCACCTGTGTCTGCATTGGGCCCTTCTGTTTTGGCGTCCTCAACTGCTAATCTTAACATTGGAGCTATTAATCCAGCTGCATCTTTTTATCAAGCTTTTGACCCCAGTCTGTCAAATCCAATGTCTCAGGTGGGAGTAACAGGGACTATCTATCCTCAGCGCCCTGGACAGATTGAATGTGATTTCTATATGAAATCTGGCATATGTAAATTTGGGGAAAGATGCAAGTACCATCACCCAATCGACCGCACAACATCACTGTCAAAACTGCAATCAAATGTTAAACTTACTGCTGCAGGGTTACCAAGAAGAGAGGGTGTTGAGATCTGTCCATATTATTTGAAAACTGCAACGTGCAAGTTTGGCGCAACATGCAAATTTGACCATCCACCACCCGGAGAAGTGATGGAGATGGCTAAGTCACAAGGAGCTTCTACAACCAATGGAGGAGAAACGGAGACCAACGAAGATGTGCCTGGTTCTGCTCCGGAACAATATTGA
Protein sequence

>MsaT042818.1

MENRIYNTSSYNIPTGAAPSTTSPTQYYDPNKMFLAHYRRTAEAAAAASSIRYSSDFDIAPPGVASRISTTNPWANAFPSAAAAVAAAATNVASAASLDLKRSSDALYHPSILSTIGQNEAWYTSNSLAKRPRYETGSTLPIYPQRPGEKDCAHYMLTRTCKFGESCKFDHPIWVPAGGIPDWKEVPNIVPSETLPERPGEPDCPFFLKTQKCKFGTKCKFNHPNVPSENADFYLKTGKCKYGAACKFHHPKDIQIQLSDDLSRNVEQTQTNSSVFDGAIGDTQSTMPLISPLSHNSKGLPVRPGEVDCPFYMKTGSCKFGASCRYNHPDMNAINPPVSALGPSVLASSTANLNIGAINPAASFYQAFDPSLSNPMSQVGVTGTIYPQRPGQIECDFYMKSGICKFGERCKYHHPIDRTTSLSKLQSNVKLTAAGLPRREGVEICPYYLKTATCKFGATCKFDHPPPGEVMEMAKSQGASTTNGGETETNEDVPGSAPEQY*