AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG042882

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG042882 MtrunA17_Chr2g0281511 94.262 122 7 0 1 122 1 122 5.37e-83 238
MsaG042882 MtrunA17_Chr2g0309121 86.885 122 15 1 1 122 1 121 3.49e-74 216
MsaG042882 MtrunA17_Chr3g0081891 95.000 80 4 0 1 80 1 80 2.77e-51 157
MsaG042882 MtrunA17_Chr7g0276661 34.615 130 67 4 7 119 38 166 9.91e-20 79.7
MsaG042882 MtrunA17_Chr0c01g0489371 43.956 91 49 1 22 110 224 314 6.46e-18 77.4
MsaG042882 MtrunA17_Chr8g0356961 42.697 89 49 1 24 110 77 165 3.13e-17 73.2
MsaG042882 MtrunA17_Chr8g0372431 28.866 97 69 0 24 120 6 102 7.42e-16 67.8
MsaG042882 MtrunA17_Chr5g0430431 36.667 90 47 1 37 116 165 254 1.23e-14 67.8
MsaG042882 MtrunA17_Chr5g0434641 37.363 91 55 1 22 110 208 298 1.53e-13 65.1
MsaG042882 MtrunA17_Chr8g0356951 40.659 91 50 3 24 110 217 307 4.21e-13 63.9
MsaG042882 MtrunA17_Chr6g0485871 29.808 104 64 2 6 108 204 299 5.22e-12 60.8
MsaG042882 MtrunA17_Chr7g0228081 30.000 80 56 0 41 120 13 92 1.95e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGTTACTACTTCTATTT+TGG 0.119273 8:+29049956 MsaT042882.1:CDS
GAAGTCAATGGTACTGATTT+AGG 0.173353 8:+29049897 MsaT042882.1:CDS
AAAATGTCGATGTTCATTAT+TGG 0.262235 8:+29049928 MsaT042882.1:CDS
TACCGGTTTCTTCATCAAAA+AGG 0.272684 8:-29050150 None:intergenic
TGTATGCTACCGTCGTTAAC+TGG 0.297739 8:+29050317 MsaT042882.1:CDS
AAGTCAATGGTACTGATTTA+GGG 0.335811 8:+29049898 MsaT042882.1:CDS
CCTCGACGTGTTGCTGAGAA+TGG 0.340072 8:+29050100 MsaT042882.1:CDS
ATCACAGTCATAAGATTTAC+CGG 0.342234 8:-29050167 None:intergenic
CGGTCAATAATCCTCCTAAT+AGG 0.355854 8:+29050293 MsaT042882.1:CDS
TTTGGATGGAAACAATGTTT+TGG 0.356448 8:+29049974 MsaT042882.1:CDS
GTACGACTTTGTTCTTGAAA+AGG 0.412286 8:+29050240 MsaT042882.1:CDS
ACGGTAGCATACATCCTATT+AGG 0.440754 8:-29050307 None:intergenic
TACGACTTTGTTCTTGAAAA+GGG 0.450393 8:+29050241 MsaT042882.1:CDS
CTTGAAAAGGGCCTAAAAGT+TGG 0.503487 8:+29050253 MsaT042882.1:CDS
GTATGCTACCGTCGTTAACT+GGG 0.522932 8:+29050318 MsaT042882.1:CDS
AGCATGCATCAGAAATACAT+AGG 0.544935 8:+29050208 MsaT042882.1:CDS
AGAAATACATAGGGAGAGGA+TGG 0.573439 8:+29050218 MsaT042882.1:CDS
GAGATTGGAGTTGAAGTCAA+TGG 0.575541 8:+29049885 MsaT042882.1:CDS
CATCAGAAATACATAGGGAG+AGG 0.580360 8:+29050214 MsaT042882.1:CDS
GCATGCATCAGAAATACATA+GGG 0.580795 8:+29050209 MsaT042882.1:CDS
GTAGCATACATCCTATTAGG+AGG 0.633251 8:-29050304 None:intergenic
CCATTCTCAGCAACACGTCG+AGG 0.662672 8:-29050100 None:intergenic
GAATCAGTCCCAGTTAACGA+CGG 0.733495 8:-29050326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 29049879 29050345 29049879 ID=MsaG042882
Chr8 mRNA 29049879 29050345 29049879 ID=MsaT042882.1;Parent=MsaG042882
Chr8 exon 29049879 29049995 29049879 ID=MsaT042882.1.exon1;Parent=MsaT042882.1
Chr8 CDS 29049879 29049995 29049879 ID=cds.MsaT042882.1;Parent=MsaT042882.1
Chr8 exon 29050094 29050345 29050094 ID=MsaT042882.1.exon2;Parent=MsaT042882.1
Chr8 CDS 29050094 29050345 29050094 ID=cds.MsaT042882.1;Parent=MsaT042882.1
Gene Sequence

>MsaT042882.1

ATGAATGAGATTGGAGTTGAAGTCAATGGTACTGATTTAGGGAATGTTCAAAATGTCGATGTTCATTATTGGAAGTATAATGTTACTACTTCTATTTTGGATGGAAACAATGTTTTGCATTTTCCTCGACGTGTTGCTGAGAATGGTTTGTATATCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACCGGTAAATCTTATGACTGTGATGTGCACACTGCGTCAAAAAGCATGCATCAGAAATACATAGGGAGAGGATGGTACGACTTTGTTCTTGAAAAGGGCCTAAAAGTTGGTGACGTTATTTTGTTCACGGTCAATAATCCTCCTAATAGGATGTATGCTACCGTCGTTAACTGGGACTGA
Protein sequence

>MsaT042882.1

MNEIGVEVNGTDLGNVQNVDVHYWKYNVTTSILDGNNVLHFPRRVAENGLYINQSKILLFDEETGKSYDCDVHTASKSMHQKYIGRGWYDFVLEKGLKVGDVILFTVNNPPNRMYATVVNWD*