Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043012 | XP_013445167.1 | 91.743 | 327 | 22 | 1 | 1 | 327 | 1 | 322 | 0.0 | 566 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043012 | sp|P55197|AF10_HUMAN | 37.297 | 185 | 98 | 6 | 130 | 304 | 25 | 201 | 2.12e-32 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043012 | tr|G7ZV42|G7ZV42_MEDTR | 91.743 | 327 | 22 | 1 | 1 | 327 | 1 | 322 | 0.0 | 566 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG043012 | TR | PHD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG004514 | MsaG043012 | 0.813650 | 3.964491e-51 | 7.407913e-49 |
| MsaG005295 | MsaG043012 | 0.812009 | 9.030473e-51 | 1.619843e-48 |
| MsaG005297 | MsaG043012 | 0.824024 | 1.786541e-53 | 4.376103e-51 |
| MsaG005540 | MsaG043012 | 0.819982 | 1.527727e-52 | 3.358449e-50 |
| MsaG005960 | MsaG043012 | 0.811170 | 1.371813e-50 | 2.410561e-48 |
| MsaG006255 | MsaG043012 | 0.840697 | 1.376521e-57 | 5.492518e-55 |
| MsaG006261 | MsaG043012 | 0.814787 | 2.229930e-51 | 4.287524e-49 |
| MsaG006314 | MsaG043012 | 0.843454 | 2.588490e-58 | 1.127793e-55 |
| MsaG007555 | MsaG043012 | 0.813790 | 3.693127e-51 | 6.925561e-49 |
| MsaG011472 | MsaG043012 | 0.807932 | 6.753173e-50 | 1.097217e-47 |
| MsaG043012 | MsaG044337 | 0.809288 | 3.476388e-50 | 5.835290e-48 |
| MsaG043012 | MsaG045182 | 0.811901 | 9.533545e-51 | 1.705552e-48 |
| MsaG043012 | MsaG046216 | 0.823301 | 2.633206e-53 | 6.324628e-51 |
| MsaG043012 | MsaG046266 | 0.808579 | 4.921784e-50 | 8.121999e-48 |
| MsaG043012 | MsaG047119 | 0.824518 | 1.368915e-53 | 3.398529e-51 |
| MsaG043012 | MsaG009508 | 0.812801 | 6.074553e-51 | 1.111251e-48 |
| MsaG043012 | MsaG022828 | 0.811129 | 1.400368e-50 | 2.458200e-48 |
| MsaG043012 | MsaG021114 | 0.816226 | 1.069963e-51 | 2.133710e-49 |
| MsaG043012 | MsaG032525 | 0.823178 | 2.811793e-53 | 6.731079e-51 |
| MsaG043012 | MsaG036760 | 0.824033 | 1.777971e-53 | 4.356165e-51 |
| MsaG043012 | MsaG043366 | 0.816253 | 1.055357e-51 | 2.105987e-49 |
| MsaG016293 | MsaG043012 | 0.807568 | 8.063192e-50 | 1.298580e-47 |
| MsaG017365 | MsaG043012 | 0.860211 | 4.756273e-63 | 3.728260e-60 |
| MsaG019409 | MsaG043012 | 0.811970 | 9.210832e-51 | 1.650620e-48 |
| MsaG026842 | MsaG043012 | 0.816269 | 1.047100e-51 | 2.090341e-49 |
| MsaG028450 | MsaG043012 | 0.830249 | 5.873457e-55 | 1.711925e-52 |
| MsaG040013 | MsaG043012 | 0.806544 | 1.324457e-49 | 2.082086e-47 |
| MsaG041096 | MsaG043012 | 0.804536 | 3.477827e-49 | 5.216136e-47 |
| MsaG041237 | MsaG043012 | 0.810968 | 1.516450e-50 | 2.651537e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043012 | MtrunA17_Chr8g0359281 | 91.743 | 327 | 22 | 1 | 1 | 327 | 1 | 322 | 0.0 | 566 |
| MsaG043012 | MtrunA17_Chr7g0221611 | 37.821 | 156 | 84 | 4 | 130 | 282 | 605 | 750 | 7.70e-22 | 96.7 |
| MsaG043012 | MtrunA17_Chr7g0275611 | 27.632 | 228 | 142 | 7 | 82 | 299 | 483 | 697 | 1.74e-16 | 80.5 |
| MsaG043012 | MtrunA17_Chr8g0347071 | 28.488 | 172 | 111 | 5 | 130 | 299 | 521 | 682 | 2.06e-15 | 77.4 |
| MsaG043012 | MtrunA17_Chr3g0126701 | 30.409 | 171 | 103 | 6 | 130 | 294 | 632 | 792 | 1.35e-13 | 71.6 |
| MsaG043012 | MtrunA17_Chr1g0147121 | 28.723 | 188 | 118 | 6 | 130 | 311 | 602 | 779 | 2.53e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043012 | AT3G14740.2 | 45.758 | 330 | 139 | 5 | 22 | 325 | 25 | 340 | 3.44e-89 | 271 |
| MsaG043012 | AT3G14740.1 | 45.758 | 330 | 137 | 5 | 22 | 325 | 25 | 338 | 5.62e-89 | 270 |
| MsaG043012 | AT2G31650.1 | 38.312 | 154 | 88 | 3 | 130 | 282 | 612 | 759 | 1.62e-26 | 110 |
| MsaG043012 | AT1G05830.2 | 37.736 | 159 | 82 | 5 | 130 | 282 | 629 | 776 | 1.07e-25 | 108 |
| MsaG043012 | AT1G05830.4 | 37.736 | 159 | 82 | 5 | 130 | 282 | 629 | 776 | 1.07e-25 | 108 |
| MsaG043012 | AT1G05830.1 | 37.736 | 159 | 82 | 5 | 130 | 282 | 629 | 776 | 1.07e-25 | 108 |
| MsaG043012 | AT1G05830.3 | 37.736 | 159 | 82 | 5 | 130 | 282 | 629 | 776 | 1.07e-25 | 108 |
| MsaG043012 | AT3G61740.1 | 32.258 | 155 | 93 | 6 | 130 | 282 | 549 | 693 | 1.94e-15 | 77.8 |
| MsaG043012 | AT3G61740.2 | 32.258 | 155 | 93 | 6 | 130 | 282 | 549 | 693 | 2.25e-15 | 77.4 |
| MsaG043012 | AT5G53430.1 | 25.847 | 236 | 149 | 9 | 91 | 311 | 563 | 787 | 1.53e-13 | 72.0 |
| MsaG043012 | AT4G27910.1 | 28.507 | 221 | 123 | 12 | 130 | 327 | 595 | 803 | 4.49e-13 | 70.5 |
Find 73 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCTTTGTATTGAGTATA+AGG | 0.260989 | 8:-33756673 | MsaT043012.1:CDS |
| CTGCGACGGTTGTAACTTAA+TGG | 0.286202 | 8:-33757066 | MsaT043012.1:CDS |
| CGTCGGTTGGGATGTTGTTT+TGG | 0.298059 | 8:+33757258 | None:intergenic |
| TCAATCCCTTCTCTCCCTTC+TGG | 0.311163 | 8:+33756825 | None:intergenic |
| AAAGGTGCTACTGTTGTTGC+TGG | 0.352947 | 8:-33756642 | MsaT043012.1:CDS |
| GGTGGTGAAGGTGGGGTTGA+AGG | 0.356961 | 8:+33757279 | None:intergenic |
| TTCTGAACAGAAGTGTGGTT+TGG | 0.363928 | 8:-33756724 | MsaT043012.1:CDS |
| AATGAATCTTAGTCGTTTGT+TGG | 0.373084 | 8:+33757469 | None:intergenic |
| TCTGAACAGAAGTGTGGTTT+GGG | 0.382510 | 8:-33756723 | MsaT043012.1:CDS |
| ATGAATCTTAGTCGTTTGTT+GGG | 0.423800 | 8:+33757470 | None:intergenic |
| CGCAACGATCTTGTATTTCC+CGG | 0.424868 | 8:+33756209 | None:intergenic |
| GGACAGAGGGAGCAACGAAT+TGG | 0.436530 | 8:+33756939 | None:intergenic |
| GTGAAGGTGGGGTTGAAGGG+CGG | 0.439165 | 8:+33757283 | None:intergenic |
| GGGGTGTTGATGATGAAGGT+GGG | 0.444598 | 8:+33757345 | None:intergenic |
| AACAAACAACAGATGGAAAG+TGG | 0.447815 | 8:-33756896 | MsaT043012.1:CDS |
| GTGGTGAAGGTGGGGTTGAA+GGG | 0.451273 | 8:+33757280 | None:intergenic |
| TTGGTGCAGAACAAGGGATC+CGG | 0.451306 | 8:-33756228 | MsaT043012.1:CDS |
| TCACAAAACCAGTCACCCTC+TGG | 0.452678 | 8:+33757002 | None:intergenic |
| TATGTATGTGGGTGTTGTGA+TGG | 0.455586 | 8:-33756762 | MsaT043012.1:CDS |
| ATGGTCCATGCTTCCTGCTA+TGG | 0.455725 | 8:-33757047 | MsaT043012.1:CDS |
| CACCAGAGGGTTACCATAGC+AGG | 0.457643 | 8:+33757034 | None:intergenic |
| GTGGGTAGAGAATAAGTAGA+TGG | 0.471895 | 8:+33757320 | None:intergenic |
| GTGTTGTGATGGATGTGCTT+TGG | 0.478647 | 8:-33756751 | MsaT043012.1:CDS |
| CTTTGTATTGAGTATAAGGA+AGG | 0.487792 | 8:-33756669 | MsaT043012.1:CDS |
| AACAGATCCCAGAGGGTGAC+TGG | 0.488130 | 8:-33757010 | MsaT043012.1:CDS |
| TATTACTTGTGGAATTAAAG+AGG | 0.496711 | 8:-33756694 | MsaT043012.1:CDS |
| GGGGTGGGTGAGGGGAGAGA+AGG | 0.500025 | 8:+33757200 | None:intergenic |
| GAGTATAAGGAAGGGAAGAA+AGG | 0.503186 | 8:-33756660 | MsaT043012.1:CDS |
| TGAGTGGAACAGCGTCGGTT+GGG | 0.509178 | 8:+33757246 | None:intergenic |
| CTGGGATCTGTTTCACCAGA+GGG | 0.510639 | 8:+33757021 | None:intergenic |
| CTCCCTCTGTCCTACGAAAG+AGG | 0.511997 | 8:-33756928 | MsaT043012.1:CDS |
| CAAGTAAAGCACAAACAAGA+TGG | 0.512592 | 8:+33756871 | None:intergenic |
| CAGCATTAGCATCTGTGCTA+TGG | 0.516709 | 8:+33757105 | None:intergenic |
| TTGGAGAAGTGTTATGTATG+TGG | 0.523141 | 8:-33756774 | MsaT043012.1:CDS |
| ATGAGTGGAACAGCGTCGGT+TGG | 0.531511 | 8:+33757245 | None:intergenic |
| TGGATCCAGAAGGGAGAGAA+GGG | 0.535229 | 8:-33756830 | MsaT043012.1:CDS |
| TCGTTTGTTGGGAGGTAGGT+CGG | 0.538544 | 8:+33757481 | None:intergenic |
| TTAGTCGTTTGTTGGGAGGT+AGG | 0.543612 | 8:+33757477 | None:intergenic |
| TGGAGAAGTGTTATGTATGT+GGG | 0.545181 | 8:-33756773 | MsaT043012.1:CDS |
| TGGTGCAGAACAAGGGATCC+GGG | 0.545804 | 8:-33756227 | MsaT043012.1:CDS |
| CAACCGTCGCAGAAAACGAT+TGG | 0.547295 | 8:+33757077 | None:intergenic |
| TTCCTGCTATGGTAACCCTC+TGG | 0.549750 | 8:-33757036 | MsaT043012.1:CDS |
| GCAATGAAACAAACAACAGA+TGG | 0.550342 | 8:-33756903 | MsaT043012.1:CDS |
| TCCCTCTTTCGTAGGACAGA+GGG | 0.550747 | 8:+33756926 | None:intergenic |
| TTCATTGCTCCCTCTTTCGT+AGG | 0.551522 | 8:+33756918 | None:intergenic |
| GTGGATCCAGAAGGGAGAGA+AGG | 0.554299 | 8:-33756831 | MsaT043012.1:CDS |
| TCCCTCTGTCCTACGAAAGA+GGG | 0.571477 | 8:-33756927 | MsaT043012.1:CDS |
| GTTCTAAGATTCCACAGAAG+AGG | 0.573200 | 8:-33756800 | MsaT043012.1:CDS |
| TTCTCCAACCACCTCTTCTG+TGG | 0.573745 | 8:+33756789 | None:intergenic |
| GATTCCACAGAAGAGGTGGT+TGG | 0.573938 | 8:-33756793 | MsaT043012.1:CDS |
| CACAAAACCAGTCACCCTCT+GGG | 0.576262 | 8:+33757003 | None:intergenic |
| AATCTTAGTCGTTTGTTGGG+AGG | 0.581292 | 8:+33757473 | None:intergenic |
| GTAGAGAATAAGTAGATGGT+GGG | 0.581521 | 8:+33757324 | None:intergenic |
| CTAAGATTCCACAGAAGAGG+TGG | 0.584859 | 8:-33756797 | MsaT043012.1:CDS |
| GGTAGAGAATAAGTAGATGG+TGG | 0.587962 | 8:+33757323 | None:intergenic |
| GGTTACCATAGCAGGAAGCA+TGG | 0.589460 | 8:+33757042 | None:intergenic |
| GGGTGTTGATGATGAAGGTG+GGG | 0.592237 | 8:+33757346 | None:intergenic |
| GTTTGTTCTGAACAGAAGTG+TGG | 0.601379 | 8:-33756729 | MsaT043012.1:CDS |
| ATCGATGAGTGGAACAGCGT+CGG | 0.605400 | 8:+33757241 | None:intergenic |
| AAGTAAAGCACAAACAAGAT+GGG | 0.608555 | 8:+33756872 | None:intergenic |
| CTCCCTCTTTCGTAGGACAG+AGG | 0.612913 | 8:+33756925 | None:intergenic |
| TCTGGGATCTGTTTCACCAG+AGG | 0.613157 | 8:+33757020 | None:intergenic |
| GAGGGAGCAACGAATTGGAT+CGG | 0.621558 | 8:+33756944 | None:intergenic |
| ACAAACAACAGATGGAAAGT+GGG | 0.626115 | 8:-33756895 | MsaT043012.1:CDS |
| AGAGAATAAGTAGATGGTGG+GGG | 0.628297 | 8:+33757326 | None:intergenic |
| TTTGTATTGAGTATAAGGAA+GGG | 0.629492 | 8:-33756668 | MsaT043012.1:CDS |
| TAGAGAATAAGTAGATGGTG+GGG | 0.637260 | 8:+33757325 | None:intergenic |
| TAGCACAGATGCTAATGCTG+AGG | 0.649193 | 8:-33757102 | MsaT043012.1:CDS |
| CTGGTGAAACAGATCCCAGA+GGG | 0.654987 | 8:-33757017 | MsaT043012.1:CDS |
| GAATTCGAAGTCGATGACGA+TGG | 0.657912 | 8:-33757146 | MsaT043012.1:CDS |
| TCGACGTTGAGATCGATGAG+TGG | 0.673931 | 8:+33757230 | None:intergenic |
| CGGGAAATACAAGATCGTTG+CGG | 0.728498 | 8:-33756208 | MsaT043012.1:CDS |
| TCTGGTGAAACAGATCCCAG+AGG | 0.763846 | 8:-33757018 | MsaT043012.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 33756191 | 33757525 | 33756191 | ID=MsaG043012 |
| Chr8 | mRNA | 33756191 | 33757525 | 33756191 | ID=MsaT043012.1;Parent=MsaG043012 |
| Chr8 | exon | 33756191 | 33756285 | 33756191 | ID=MsaT043012.1.exon2;Parent=MsaT043012.1 |
| Chr8 | CDS | 33756191 | 33756285 | 33756191 | ID=cds.MsaT043012.1;Parent=MsaT043012.1 |
| Chr8 | exon | 33756637 | 33757525 | 33756637 | ID=MsaT043012.1.exon1;Parent=MsaT043012.1 |
| Chr8 | CDS | 33756637 | 33757525 | 33756637 | ID=cds.MsaT043012.1;Parent=MsaT043012.1 |
| Gene Sequence |
| Protein sequence |
