AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043046

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043046 MtrunA17_Chr8g0359921 96.269 268 10 0 46 313 62 329 0.0 527
MsaG043046 MtrunA17_Chr2g0318081 59.441 286 91 8 45 310 63 343 2.59e-100 298
MsaG043046 MtrunA17_Chr7g0252971 44.397 232 90 9 45 250 55 273 8.34e-49 166
MsaG043046 MtrunA17_Chr3g0124761 71.818 110 29 2 47 155 56 164 7.20e-48 162
MsaG043046 MtrunA17_Chr4g0047841 60.870 138 31 4 45 159 62 199 1.53e-47 162
MsaG043046 MtrunA17_Chr2g0318101 64.228 123 33 3 45 156 62 184 2.91e-47 162
MsaG043046 MtrunA17_Chr5g0417791 64.545 110 37 1 46 153 51 160 1.58e-46 157
MsaG043046 MtrunA17_Chr8g0384721 65.854 123 35 3 47 163 56 177 3.64e-46 157
MsaG043046 MtrunA17_Chr4g0042561 64.762 105 35 1 46 148 57 161 4.15e-45 154
MsaG043046 MtrunA17_Chr7g0263931 59.712 139 46 4 45 179 58 190 1.48e-44 154
MsaG043046 MtrunA17_Chr3g0130731 71.296 108 23 3 51 154 60 163 4.02e-44 152
MsaG043046 MtrunA17_Chr2g0326361 61.475 122 38 3 46 158 62 183 4.50e-43 150
MsaG043046 MtrunA17_Chr6g0453451 55.046 109 49 0 46 154 68 176 1.30e-37 136
MsaG043046 MtrunA17_Chr4g0060931 56.881 109 47 0 46 154 65 173 5.17e-37 135
MsaG043046 MtrunA17_Chr6g0484441 47.183 142 64 3 46 187 53 183 5.52e-37 134
MsaG043046 MtrunA17_Chr3g0091921 56.881 109 47 0 46 154 64 172 4.99e-36 131
MsaG043046 MtrunA17_Chr7g0253011 55.752 113 46 1 46 154 65 177 9.11e-36 132
MsaG043046 MtrunA17_Chr2g0309891 54.054 111 46 2 53 163 63 168 1.58e-35 131
MsaG043046 MtrunA17_Chr2g0312321 40.237 169 92 4 47 212 58 220 3.07e-35 129
MsaG043046 MtrunA17_Chr3g0113001 46.763 139 71 3 47 182 14 152 1.10e-34 127
MsaG043046 MtrunA17_Chr6g0461661 54.701 117 51 1 46 160 66 182 4.39e-34 128
MsaG043046 MtrunA17_Chr4g0076731 59.223 103 37 2 47 148 103 201 1.06e-33 128
MsaG043046 MtrunA17_Chr6g0481091 54.639 97 42 1 53 149 63 157 1.45e-33 123
MsaG043046 MtrunA17_Chr5g0417001 56.303 119 43 6 47 156 63 181 2.31e-33 126
MsaG043046 MtrunA17_Chr3g0139331 54.054 111 45 2 46 154 59 165 5.45e-33 124
MsaG043046 MtrunA17_Chr2g0316971 54.630 108 47 1 46 151 71 178 8.87e-33 124
MsaG043046 MtrunA17_Chr5g0433781 56.000 100 42 1 51 150 61 158 1.20e-32 127
MsaG043046 MtrunA17_Chr4g0017921 56.122 98 38 1 51 148 64 156 2.15e-32 123
MsaG043046 MtrunA17_Chr3g0109821 59.184 98 39 1 53 150 63 159 2.19e-32 126
MsaG043046 MtrunA17_Chr6g0453131 52.212 113 46 2 47 151 64 176 3.49e-32 123
MsaG043046 MtrunA17_Chr8g0361731 51.667 120 48 5 47 156 63 182 6.92e-32 122
MsaG043046 MtrunA17_Chr1g0200561 40.000 165 80 4 51 215 75 220 7.79e-32 121
MsaG043046 MtrunA17_Chr2g0309331 51.818 110 45 3 51 156 66 171 8.54e-32 121
MsaG043046 MtrunA17_Chr8g0346001 51.429 105 44 2 51 148 71 175 1.08e-31 122
MsaG043046 MtrunA17_Chr1g0147421 52.252 111 47 2 46 154 76 182 1.09e-31 121
MsaG043046 MtrunA17_Chr7g0217721 46.281 121 52 1 47 154 68 188 6.20e-31 119
MsaG043046 MtrunA17_Chr2g0311661 43.949 157 69 5 50 191 74 226 1.66e-30 116
MsaG043046 MtrunA17_Chr4g0038751 50.926 108 43 2 47 151 64 164 2.63e-29 113
MsaG043046 MtrunA17_Chr4g0018391 29.779 272 151 8 51 298 63 318 2.65e-29 114
MsaG043046 MtrunA17_Chr7g0217711 59.770 87 34 1 47 132 68 154 2.70e-29 110
MsaG043046 MtrunA17_Chr4g0056541 43.478 115 61 1 51 165 63 173 2.74e-29 115
MsaG043046 MtrunA17_Chr3g0128111 39.744 156 73 4 53 207 99 234 5.66e-29 111
MsaG043046 MtrunA17_Chr5g0428991 37.838 185 97 5 53 228 82 257 1.26e-28 115
MsaG043046 MtrunA17_Chr8g0372121 47.170 106 52 1 48 153 59 160 1.73e-28 111
MsaG043046 MtrunA17_Chr3g0128151 40.506 158 80 4 51 207 77 221 3.33e-28 114
MsaG043046 MtrunA17_Chr2g0311651 50.794 126 47 5 46 156 69 194 3.51e-28 110
MsaG043046 MtrunA17_Chr5g0400461 50.000 106 41 2 51 149 61 161 4.40e-28 111
MsaG043046 MtrunA17_Chr5g0405211 46.154 104 52 1 51 154 64 163 7.77e-28 110
MsaG043046 MtrunA17_Chr7g0214071 52.830 106 44 3 51 154 65 166 7.95e-28 108
MsaG043046 MtrunA17_Chr3g0092671 46.364 110 56 1 45 154 55 161 3.06e-27 111
MsaG043046 MtrunA17_Chr5g0442771 44.755 143 69 3 50 182 70 212 7.16e-27 107
MsaG043046 MtrunA17_Chr5g0398971 48.485 99 47 2 51 148 63 158 5.19e-26 105
MsaG043046 MtrunA17_Chr3g0113031 42.857 126 68 3 61 182 3 128 7.23e-26 103
MsaG043046 MtrunA17_Chr1g0183741 42.424 132 66 3 47 176 59 182 1.07e-25 103
MsaG043046 MtrunA17_Chr8g0364791 45.455 99 52 1 50 148 60 156 1.08e-25 106
MsaG043046 MtrunA17_Chr1g0201021 47.475 99 50 2 50 148 73 169 1.46e-25 106
MsaG043046 MtrunA17_Chr3g0144271 59.494 79 30 1 47 123 73 151 3.56e-25 104
MsaG043046 MtrunA17_Chr7g0261411 49.020 102 44 2 47 146 59 154 4.50e-25 102
MsaG043046 MtrunA17_Chr1g0197351 46.847 111 55 1 44 150 90 200 4.58e-25 104
MsaG043046 MtrunA17_Chr1g0197361 44.643 112 59 1 43 154 56 164 5.18e-25 105
MsaG043046 MtrunA17_Chr2g0321741 42.520 127 53 4 51 177 46 152 9.86e-25 102
MsaG043046 MtrunA17_Chr3g0130101 43.396 106 58 1 43 148 54 157 4.48e-24 100
MsaG043046 MtrunA17_Chr2g0321631 48.454 97 48 2 52 146 64 160 5.19e-24 98.2
MsaG043046 MtrunA17_Chr4g0054791 49.057 106 47 3 53 154 63 165 1.03e-23 99.0
MsaG043046 MtrunA17_Chr8g0384201 42.424 99 55 1 50 148 60 156 1.15e-23 100
MsaG043046 MtrunA17_Chr3g0131911 46.018 113 53 2 48 152 62 174 2.18e-23 96.3
MsaG043046 MtrunA17_Chr8g0385991 45.000 100 50 2 51 145 65 164 1.09e-22 94.4
MsaG043046 MtrunA17_Chr4g0026141 43.878 98 53 2 51 146 63 160 1.84e-22 94.4
MsaG043046 MtrunA17_Chr7g0243371 58.667 75 29 2 51 123 17 91 7.30e-22 89.0
MsaG043046 MtrunA17_Chr7g0266701 41.176 102 56 3 52 149 64 165 8.94e-22 92.4
MsaG043046 MtrunA17_Chr8g0345671 46.939 98 42 4 50 145 66 155 3.49e-19 87.4
MsaG043046 MtrunA17_Chr8g0345691 35.185 162 86 6 53 208 70 218 4.09e-16 78.6
MsaG043046 MtrunA17_Chr4g0050701 35.514 107 55 3 53 153 66 164 4.32e-16 75.1
MsaG043046 MtrunA17_Chr8g0345721 45.205 73 37 2 51 121 120 191 4.13e-14 70.1
MsaG043046 MtrunA17_Chr8g0345741 38.776 98 46 4 51 146 47 132 6.52e-14 68.2
MsaG043046 MtrunA17_Chr8g0345711 34.615 104 56 4 51 143 68 170 3.33e-12 63.9
MsaG043046 MtrunA17_Chr8g0345731 35.870 92 52 3 53 137 82 173 2.22e-11 61.6
MsaG043046 MtrunA17_Chr3g0130321 31.210 157 88 7 50 195 61 208 4.85e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043046 AT4G27410.2 58.577 239 71 7 45 279 61 275 1.52e-76 236
MsaG043046 AT4G27410.3 58.403 238 71 7 46 279 79 292 1.34e-75 234
MsaG043046 AT1G52890.1 60.369 217 69 7 45 247 61 274 2.23e-72 226
MsaG043046 AT3G15500.1 60.784 204 63 4 45 235 61 260 1.04e-70 222
MsaG043046 AT3G04070.2 67.797 118 28 3 45 152 57 174 5.84e-48 164
MsaG043046 AT1G61110.1 64.615 130 35 3 45 163 63 192 7.20e-48 163
MsaG043046 AT1G01720.1 69.912 113 31 2 47 157 56 167 1.51e-47 161
MsaG043046 AT5G08790.1 60.938 128 46 2 49 173 58 184 7.20e-47 159
MsaG043046 AT1G69490.1 69.369 111 30 2 46 153 57 166 1.42e-46 158
MsaG043046 AT3G15510.1 64.754 122 28 4 45 153 64 183 9.19e-46 159
MsaG043046 AT3G04070.1 62.687 134 24 5 45 152 57 190 1.15e-45 158
MsaG043046 AT5G63790.1 61.364 132 41 3 47 172 99 226 1.63e-45 157
MsaG043046 AT5G63790.2 61.364 132 41 3 47 172 109 236 2.03e-45 157
MsaG043046 AT1G77450.1 50.000 170 78 3 47 215 59 222 5.58e-45 154
MsaG043046 AT1G52880.1 67.797 118 28 4 45 152 64 181 1.94e-41 146
MsaG043046 AT5G61430.2 57.895 114 47 1 47 160 2 114 4.74e-38 136
MsaG043046 AT3G04060.1 51.471 136 59 2 47 181 69 198 1.25e-37 137
MsaG043046 AT5G18270.2 49.333 150 72 3 45 191 68 216 1.61e-37 136
MsaG043046 AT3G18400.1 45.455 154 75 3 45 198 52 196 1.85e-37 135
MsaG043046 AT5G07680.2 57.798 109 46 0 46 154 51 159 2.82e-37 135
MsaG043046 AT5G61430.1 57.895 114 47 1 47 160 65 177 2.83e-37 136
MsaG043046 AT5G07680.1 57.798 109 46 0 46 154 65 173 3.13e-37 135
MsaG043046 AT5G18270.1 48.322 149 73 3 46 191 69 216 1.24e-36 134
MsaG043046 AT5G39610.1 56.881 109 47 0 46 154 68 176 3.48e-36 132
MsaG043046 AT2G02450.1 51.908 131 56 4 45 172 98 224 9.07e-35 130
MsaG043046 AT2G02450.2 51.908 131 56 4 45 172 98 224 1.02e-34 130
MsaG043046 AT2G24430.2 54.867 113 46 2 46 156 64 173 1.36e-34 128
MsaG043046 AT2G24430.1 54.867 113 46 2 46 156 64 173 1.36e-34 128
MsaG043046 AT3G12977.2 43.972 141 69 4 47 187 14 144 2.18e-34 125
MsaG043046 AT1G56010.1 53.774 106 46 2 47 150 2 106 5.04e-34 125
MsaG043046 AT5G39820.1 50.000 134 44 4 47 157 69 202 2.16e-33 125
MsaG043046 AT3G12977.1 43.972 141 69 4 47 187 67 197 2.17e-33 124
MsaG043046 AT1G56010.2 53.774 106 46 2 47 150 69 173 2.19e-33 125
MsaG043046 AT3G15170.1 45.070 142 74 2 46 183 68 209 2.63e-33 125
MsaG043046 AT5G53950.1 44.755 143 71 2 46 180 65 207 4.13e-33 125
MsaG043046 AT2G46770.1 44.371 151 69 6 51 186 72 222 4.98e-33 125
MsaG043046 AT3G29035.1 54.545 110 50 0 46 155 72 181 1.08e-32 123
MsaG043046 AT3G17730.1 54.639 97 42 1 53 149 63 157 1.11e-32 121
MsaG043046 AT2G33480.3 55.882 102 43 1 51 152 8 107 2.81e-32 119
MsaG043046 AT4G10350.2 55.102 98 42 1 51 148 18 113 4.84e-32 121
MsaG043046 AT1G26870.1 54.464 112 42 3 50 152 68 179 7.46e-32 123
MsaG043046 AT2G43000.1 45.205 146 69 3 47 183 67 210 9.10e-32 120
MsaG043046 AT1G65910.1 53.922 102 45 1 53 154 63 162 1.55e-31 124
MsaG043046 AT4G10350.1 55.102 98 42 1 51 148 65 160 1.55e-31 120
MsaG043046 AT2G33480.1 55.882 102 43 1 51 152 65 164 1.99e-31 119
MsaG043046 AT1G76420.1 55.238 105 40 3 47 148 71 171 3.09e-31 120
MsaG043046 AT3G61910.1 40.000 155 68 4 51 190 67 211 4.38e-31 119
MsaG043046 AT1G33280.1 55.102 98 39 1 51 148 64 156 6.64e-31 118
MsaG043046 AT2G33480.2 54.808 104 39 2 51 152 65 162 1.43e-30 116
MsaG043046 AT5G13180.1 57.547 106 40 4 51 154 64 166 1.65e-30 116
MsaG043046 AT5G62380.2 41.558 154 78 4 51 203 63 205 3.93e-30 117
MsaG043046 AT5G62380.1 41.558 154 78 4 51 203 63 205 3.93e-30 117
MsaG043046 AT1G79580.3 50.450 111 48 3 40 148 61 166 5.21e-30 117
MsaG043046 AT1G79580.5 50.450 111 48 3 40 148 61 166 5.21e-30 117
MsaG043046 AT1G79580.4 50.450 111 48 3 40 148 61 166 5.21e-30 117
MsaG043046 AT1G79580.2 50.450 111 48 3 40 148 61 166 5.21e-30 117
MsaG043046 AT1G79580.1 50.450 111 48 3 40 148 61 166 5.21e-30 117
MsaG043046 AT3G03200.1 54.082 98 44 1 51 148 61 157 6.83e-30 118
MsaG043046 AT5G17260.1 54.082 98 44 1 51 148 61 157 8.60e-30 118
MsaG043046 AT3G10480.1 50.943 106 51 1 45 150 76 180 8.71e-30 118
MsaG043046 AT1G71930.2 45.283 106 54 1 51 156 65 166 9.57e-30 115
MsaG043046 AT1G71930.1 45.283 106 54 1 51 156 65 166 9.57e-30 115
MsaG043046 AT1G32770.1 47.170 106 48 1 51 148 72 177 1.52e-29 115
MsaG043046 AT3G10480.3 50.943 106 51 1 45 150 109 213 2.15e-29 117
MsaG043046 AT3G10480.2 51.887 106 49 2 45 150 76 179 2.25e-29 117
MsaG043046 AT2G17040.1 39.773 176 80 7 47 207 55 219 3.10e-29 113
MsaG043046 AT1G62700.1 49.505 101 47 1 48 148 60 156 3.39e-29 115
MsaG043046 AT1G62700.2 49.505 101 47 1 48 148 60 156 3.39e-29 115
MsaG043046 AT3G10490.1 50.000 100 49 1 51 150 82 180 1.39e-28 110
MsaG043046 AT1G33060.2 50.980 102 48 1 51 152 79 178 2.23e-28 115
MsaG043046 AT1G33060.1 50.980 102 48 1 51 152 79 178 2.23e-28 115
MsaG043046 AT4G35580.3 55.208 96 41 1 53 148 66 159 3.37e-28 114
MsaG043046 AT4G35580.2 55.208 96 41 1 53 148 66 159 3.63e-28 114
MsaG043046 AT2G18060.3 40.909 132 73 2 51 181 65 192 3.71e-28 112
MsaG043046 AT2G18060.1 40.909 132 73 2 51 181 65 192 3.71e-28 112
MsaG043046 AT4G35580.1 55.208 96 41 1 53 148 66 159 4.21e-28 114
MsaG043046 AT3G12910.1 46.923 130 54 3 51 167 75 202 4.79e-28 110
MsaG043046 AT1G12260.2 43.243 111 59 1 51 161 63 169 5.13e-28 111
MsaG043046 AT3G10490.2 48.113 106 54 1 45 150 76 180 5.53e-28 113
MsaG043046 AT5G24590.2 44.697 132 66 3 51 179 68 195 6.36e-28 112
MsaG043046 AT1G12260.1 48.980 98 46 1 51 148 63 156 6.68e-28 112
MsaG043046 AT1G34180.3 39.869 153 74 4 50 200 70 206 8.48e-28 112
MsaG043046 AT1G34180.4 39.869 153 74 4 50 200 82 218 1.02e-27 112
MsaG043046 AT5G66300.1 46.296 108 51 2 51 158 68 168 1.03e-27 109
MsaG043046 AT4G17980.1 52.525 99 45 1 53 149 63 161 1.09e-27 108
MsaG043046 AT1G54330.1 52.525 99 42 1 51 149 61 154 1.54e-27 109
MsaG043046 AT4G17980.2 52.525 99 45 1 53 149 63 161 1.68e-27 108
MsaG043046 AT1G34180.2 39.869 153 74 4 50 200 82 218 2.33e-27 112
MsaG043046 AT1G34180.1 39.869 153 74 4 50 200 70 206 2.55e-27 112
MsaG043046 AT5G04410.1 48.649 111 55 1 44 154 57 165 3.31e-27 111
MsaG043046 AT3G10500.1 48.673 113 52 2 44 154 57 165 3.62e-27 111
MsaG043046 AT4G36160.1 50.000 96 44 1 53 148 68 159 4.07e-27 109
MsaG043046 AT4G36160.3 50.000 96 44 1 53 148 68 159 4.07e-27 109
MsaG043046 AT4G36160.2 50.000 96 44 1 53 148 72 163 4.61e-27 109
MsaG043046 AT5G46590.1 53.465 101 43 3 51 149 61 159 2.32e-26 105
MsaG043046 AT1G32510.1 45.763 118 54 3 51 160 61 176 2.81e-26 105
MsaG043046 AT2G43000.2 43.609 133 64 3 60 183 2 132 8.12e-26 102
MsaG043046 AT1G34190.1 42.373 118 62 2 39 154 59 172 1.14e-25 107
MsaG043046 AT4G28530.1 50.000 108 46 3 47 152 75 176 1.20e-25 105
MsaG043046 AT1G32870.2 46.552 116 60 1 39 154 53 166 7.30e-25 104
MsaG043046 AT1G32870.1 46.552 116 60 1 39 154 53 166 7.34e-25 104
MsaG043046 AT1G32870.3 50.000 104 50 1 51 154 101 202 9.71e-25 104
MsaG043046 AT3G49530.2 49.000 100 46 3 51 148 68 164 2.70e-24 102
MsaG043046 AT3G49530.1 49.000 100 46 3 51 148 68 164 4.35e-24 102
MsaG043046 AT5G46590.2 59.211 76 30 1 51 125 61 136 5.52e-24 99.4
MsaG043046 AT4G27410.1 48.529 136 49 6 118 249 1 119 1.67e-23 95.1
MsaG043046 AT5G64060.1 40.566 106 61 1 44 149 54 157 1.18e-22 97.1
MsaG043046 AT3G44350.2 41.748 103 58 2 52 153 64 165 3.36e-22 93.6
MsaG043046 AT5G22380.1 34.266 143 82 2 52 193 65 196 4.26e-22 93.2
MsaG043046 AT3G44290.2 41.748 103 50 2 51 153 68 160 4.92e-22 92.8
MsaG043046 AT5G22290.1 38.194 144 70 5 51 184 75 209 5.74e-22 94.7
MsaG043046 AT5G09330.5 41.000 100 57 1 50 149 60 157 6.60e-22 96.3
MsaG043046 AT5G09330.2 41.000 100 57 1 50 149 60 157 6.60e-22 96.3
MsaG043046 AT5G09330.4 41.000 100 57 1 50 149 60 157 6.60e-22 96.3
MsaG043046 AT5G09330.3 41.000 100 57 1 50 149 60 157 6.60e-22 96.3
MsaG043046 AT5G09330.1 41.000 100 57 1 50 149 60 157 6.60e-22 96.3
MsaG043046 AT2G27300.1 43.519 108 48 2 51 154 68 166 8.10e-22 94.4
MsaG043046 AT3G44290.1 41.748 103 50 2 51 153 68 160 2.94e-21 92.8
MsaG043046 AT3G10490.4 48.750 80 40 1 71 150 2 80 1.08e-19 88.6
MsaG043046 AT3G10490.3 48.750 80 40 1 71 150 2 80 1.08e-19 88.6
MsaG043046 AT3G44350.1 46.753 77 40 1 52 127 64 140 5.69e-19 82.8
MsaG043046 AT4G01540.3 43.182 88 47 1 39 123 49 136 3.30e-17 82.0
MsaG043046 AT4G01540.4 43.182 88 47 1 39 123 49 136 5.79e-17 81.3
MsaG043046 AT4G01540.1 43.182 88 47 1 39 123 49 136 6.00e-17 81.6
MsaG043046 AT4G01520.1 42.045 88 48 1 39 123 49 136 6.14e-17 80.1
MsaG043046 AT4G01540.2 43.182 88 47 1 39 123 49 136 7.13e-17 81.3
MsaG043046 AT2G18060.2 37.383 107 62 2 76 181 2 104 8.44e-17 79.3
MsaG043046 AT3G04420.2 42.308 78 45 0 44 121 53 130 4.09e-15 74.7
MsaG043046 AT3G04420.1 42.308 78 45 0 44 121 53 130 7.39e-15 74.7
MsaG043046 AT4G01550.1 28.431 204 117 6 45 233 54 243 1.08e-14 74.7
MsaG043046 AT5G04395.1 44.737 76 39 1 51 123 94 169 6.55e-14 69.3
MsaG043046 AT5G22290.2 37.288 118 55 5 77 184 2 110 1.08e-13 70.1
MsaG043046 AT5G64530.1 31.579 152 63 6 52 186 57 184 6.65e-13 66.6
MsaG043046 AT1G02220.1 37.179 78 46 1 44 121 53 127 6.23e-11 63.2

Find 96 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATTTGGGTTTGGGTATT+CGG 0.141255 8:-34410306 MsaT043046.1:CDS
CTCTTCGTCAGTGGGGTTCT+TGG 0.206001 8:+34410418 None:intergenic
GAGTTGCTGGTTCAGGTTAC+TGG 0.211622 8:-34411091 MsaT043046.1:CDS
TTGGGTGACCCATTTGGGTT+TGG 0.232329 8:-34410314 MsaT043046.1:CDS
GGTGCAGGCAAGGCGAATTT+TGG 0.246467 8:-34411179 MsaT043046.1:intron
TGGGTGACCCATTTGGGTTT+GGG 0.283899 8:-34410313 MsaT043046.1:CDS
AGAACCATTGCTGCATTCTT+TGG 0.294749 8:+34410736 None:intergenic
TCGGGCTTGGGTGACCCATT+TGG 0.306880 8:-34410320 MsaT043046.1:CDS
CGGGCTTGGGTGACCCATTT+GGG 0.318140 8:-34410319 MsaT043046.1:CDS
ATTGAATCCTCACCGGAGTT+TGG 0.319990 8:-34410539 MsaT043046.1:CDS
CCATTTGGGTTTGGGTATTC+GGG 0.326537 8:-34410305 MsaT043046.1:CDS
CGAAATGTTGGACTCGTTCC+TGG 0.353931 8:-34410687 MsaT043046.1:CDS
CTGTTTGGTCTAGAACCATT+TGG 0.354289 8:+34411113 None:intergenic
CCAAACAGAGTTGCTGGTTC+AGG 0.354563 8:-34411098 MsaT043046.1:CDS
TTATGTTGATATAAACTAGA+AGG 0.363334 8:+34410359 None:intergenic
AGCTCTGATCATCAATTTCC+AGG 0.378659 8:+34410669 None:intergenic
TGGGTTTATGAGGGAGGAAG+GGG 0.396154 8:-34412926 MsaT043046.1:CDS
CATTCTTTGGTTGAAAATGT+TGG 0.404347 8:+34410749 None:intergenic
TTTGGATATTTCCTGTCCCT+TGG 0.404470 8:+34411131 None:intergenic
TCTCCTCTTGCTGTAGCATT+CGG 0.405277 8:+34410621 None:intergenic
AAAGCGGAATGAGGACTAAC+CGG 0.407018 8:-34410391 MsaT043046.1:CDS
CTTTGAACCTCTTCGTCAGT+GGG 0.407280 8:+34410410 None:intergenic
TCCTCACCGGAGTTTGGATT+AGG 0.414541 8:-34410533 MsaT043046.1:CDS
CCCGAATACCCAAACCCAAA+TGG 0.416032 8:+34410305 None:intergenic
CGTGCCACCGTTTGGTAACA+TGG 0.418390 8:-34410456 MsaT043046.1:CDS
CCTGAACCAGCAACTCTGTT+TGG 0.420404 8:+34411098 None:intergenic
TCTAGACCAAACAGAGTTGC+TGG 0.423965 8:-34411104 MsaT043046.1:CDS
ATTGATGCAGCTTGATGATT+GGG 0.428244 8:-34410819 MsaT043046.1:intron
TATTGATGCAGCTTGATGAT+TGG 0.443903 8:-34410820 MsaT043046.1:intron
TGGATTAGGAAACCAGAGTC+AGG 0.444473 8:-34410519 MsaT043046.1:CDS
CTACAGCAAGAGGAGAAGTT+CGG 0.456841 8:-34410614 MsaT043046.1:CDS
TACAGCAAGAGGAGAAGTTC+GGG 0.459516 8:-34410613 MsaT043046.1:CDS
GTTCCTTCCGTGCCACCGTT+TGG 0.466910 8:-34410464 MsaT043046.1:CDS
CCGAATACCCAAACCCAAAT+GGG 0.471769 8:+34410306 None:intergenic
GGGTTTCACAACATGGGTGC+TGG 0.473359 8:-34410593 MsaT043046.1:CDS
TCAGTCGAGATGGGTTTATG+AGG 0.473666 8:-34412936 MsaT043046.1:CDS
ACAACTGAAGGTAGAAAAGT+TGG 0.474990 8:-34411041 MsaT043046.1:CDS
AACTAGAAGGGTCGGTGACC+CGG 0.495029 8:+34410372 None:intergenic
AGTCAGGGGATGCTGAATTA+CGG 0.495349 8:-34410503 MsaT043046.1:CDS
ATGGGTTTATGAGGGAGGAA+GGG 0.496143 8:-34412927 MsaT043046.1:CDS
GGGTTTATGAGGGAGGAAGG+GGG 0.496295 8:-34412925 MsaT043046.1:CDS
GCTTCGCGAAAACACAATCT+TGG 0.501596 8:-34410930 MsaT043046.1:CDS
GTCCCAAGTGGACGAAATGT+TGG 0.503078 8:-34410699 MsaT043046.1:CDS
GAAGTTCGGGTTTCACAACA+TGG 0.506769 8:-34410600 MsaT043046.1:CDS
GTCTCATGCATCAGTCGAGA+TGG 0.508089 8:-34412946 MsaT043046.1:CDS
GATGGGTTTATGAGGGAGGA+AGG 0.511891 8:-34412928 MsaT043046.1:CDS
TATATTGGCAAAGCACCAAA+AGG 0.512258 8:-34410996 MsaT043046.1:CDS
AGTCCAACATTTCGTCCACT+TGG 0.517541 8:+34410696 None:intergenic
ATTTCTTCAGTCCAAGGGAC+AGG 0.517559 8:-34411142 MsaT043046.1:CDS
AATTCAGCATCCCCTGACTC+TGG 0.524401 8:+34410507 None:intergenic
GAGACATCGTGGAGTGGAGG+CGG 0.526817 8:+34412964 None:intergenic
GACAAAATTATCACAACTGA+AGG 0.526909 8:-34411053 MsaT043046.1:CDS
GTCCAACATTTCGTCCACTT+GGG 0.527160 8:+34410697 None:intergenic
GCTTTGAACCTCTTCGTCAG+TGG 0.531257 8:+34410409 None:intergenic
TCAACCAAAGAATGCAGCAA+TGG 0.536044 8:-34410740 MsaT043046.1:CDS
GTCTGACTCACGAGGAAGGA+AGG 0.537715 8:-34412903 MsaT043046.1:CDS
GTGAGGATTCAATATCTGTC+GGG 0.549879 8:+34410549 None:intergenic
ATCAGAGCTTTGCATTGCCG+CGG 0.552759 8:-34410655 MsaT043046.1:CDS
AAGAGGTTCAAAGCGGAATG+AGG 0.554588 8:-34410400 MsaT043046.1:CDS
AATGGTATTTCTTCAGTCCA+AGG 0.554883 8:-34411148 MsaT043046.1:CDS
GACAAGTCCATGTTACCAAA+CGG 0.557895 8:+34410449 None:intergenic
TTGATATAAACTAGAAGGGT+CGG 0.560325 8:+34410364 None:intergenic
TCCACTTGGGACGACGATGA+AGG 0.563000 8:+34410710 None:intergenic
GGTGAGGATTCAATATCTGT+CGG 0.563156 8:+34410548 None:intergenic
TATGTTGATATAAACTAGAA+GGG 0.564471 8:+34410360 None:intergenic
GCCTTCATCGTCGTCCCAAG+TGG 0.565098 8:-34410711 MsaT043046.1:CDS
AAGCGGAATGAGGACTAACC+GGG 0.566841 8:-34410390 MsaT043046.1:CDS
ACTCCGAATGCTACAGCAAG+AGG 0.568493 8:-34410624 MsaT043046.1:CDS
TCAGAGCTTTGCATTGCCGC+GGG 0.574657 8:-34410654 MsaT043046.1:CDS
TTACCAAACGGTGGCACGGA+AGG 0.576728 8:+34410461 None:intergenic
TCTCATGCATCAGTCGAGAT+GGG 0.577554 8:-34412945 MsaT043046.1:CDS
GGATTAGGAAACCAGAGTCA+GGG 0.583546 8:-34410518 MsaT043046.1:CDS
CAAGAACCCCACTGACGAAG+AGG 0.586015 8:-34410417 MsaT043046.1:CDS
GAGGCGGTGCACCACATCAG+TGG 0.586232 8:+34412980 None:intergenic
ATGGTATTTCTTCAGTCCAA+GGG 0.588129 8:-34411147 MsaT043046.1:CDS
AGGGACAGGAAATATCCAAA+TGG 0.592563 8:-34411128 MsaT043046.1:CDS
CAGTCGAGATGGGTTTATGA+GGG 0.605507 8:-34412935 MsaT043046.1:CDS
ACTCACGAGGAAGGAAGGAA+AGG 0.606550 8:-34412898 MsaT043046.1:CDS
CATGAGACATCGTGGAGTGG+AGG 0.610413 8:+34412961 None:intergenic
CATGTTACCAAACGGTGGCA+CGG 0.615690 8:+34410457 None:intergenic
TCAGGTTACTGGAAAGCCAC+AGG 0.616957 8:-34411080 MsaT043046.1:CDS
TGGTAACATGGACTTGTCAG+CGG 0.622784 8:-34410444 MsaT043046.1:CDS
GACAGATATTGAATCCTCAC+CGG 0.626126 8:-34410546 MsaT043046.1:CDS
GCGGTGCACCACATCAGTGG+TGG 0.626432 8:+34412983 None:intergenic
GAAGAACACCACCACTGATG+TGG 0.627973 8:-34412991 MsaT043046.1:CDS
GATTAGGAAACCAGAGTCAG+GGG 0.641898 8:-34410517 MsaT043046.1:CDS
TCGAGATGGGTTTATGAGGG+AGG 0.644586 8:-34412932 MsaT043046.1:CDS
TTTGAACCTCTTCGTCAGTG+GGG 0.654075 8:+34410411 None:intergenic
TGGTTTCCTAATCCAAACTC+CGG 0.659176 8:+34410527 None:intergenic
TCCTAATCCAAACTCCGGTG+AGG 0.662436 8:+34410532 None:intergenic
ATGCATGAGACATCGTGGAG+TGG 0.675693 8:+34412958 None:intergenic
AAGTTCGGGTTTCACAACAT+GGG 0.678618 8:-34410599 MsaT043046.1:CDS
GACTGATGCATGAGACATCG+TGG 0.679827 8:+34412953 None:intergenic
ATTCGGAGTGATGTTACCCG+CGG 0.710388 8:+34410638 None:intergenic
AAGTCCATGTTACCAAACGG+TGG 0.727825 8:+34410452 None:intergenic
ACTGACGAAGAGGTTCAAAG+CGG 0.781762 8:-34410407 MsaT043046.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 34410298 34413020 34410298 ID=MsaG043046
Chr8 mRNA 34410298 34413020 34410298 ID=MsaT043046.1;Parent=MsaG043046
Chr8 exon 34410298 34410831 34410298 ID=MsaT043046.1.exon3;Parent=MsaT043046.1
Chr8 CDS 34410298 34410831 34410298 ID=cds.MsaT043046.1;Parent=MsaT043046.1
Chr8 exon 34410920 34411194 34410920 ID=MsaT043046.1.exon2;Parent=MsaT043046.1
Chr8 CDS 34410920 34411194 34410920 ID=cds.MsaT043046.1;Parent=MsaT043046.1
Chr8 exon 34412888 34413020 34412888 ID=MsaT043046.1.exon1;Parent=MsaT043046.1
Chr8 CDS 34412888 34413020 34412888 ID=cds.MsaT043046.1;Parent=MsaT043046.1
Gene Sequence

>MsaT043046.1

ATGGAAGGAAGAACACCACCACTGATGTGGTGCACCGCCTCCACTCCACGATGTCTCATGCATCAGTCGAGATGGGTTTATGAGGGAGGAAGGGGGTCTGACTCACGAGGAAGGAAGGAAAGGAAAAAGAAAAGCAAGGCGAATTTTGGTGAGAAAGAATGGTATTTCTTCAGTCCAAGGGACAGGAAATATCCAAATGGTTCTAGACCAAACAGAGTTGCTGGTTCAGGTTACTGGAAAGCCACAGGAACAGACAAAATTATCACAACTGAAGGTAGAAAAGTTGGCATAAAAAAAGCACTTGTTTTCTATATTGGCAAAGCACCAAAAGGAACAAAAACTAATTGGATTATGCATGAGTATCGTCTTCTTGATGCTTCGCGAAAACACAATCTTGGTAGCACAAAACTTGATGATTGGGTTTTGTGTCGAATATACAAGAAAAACTCAAGCTCGCAAAAGGAAATTCCAACATTTTCAACCAAAGAATGCAGCAATGGTTCTTCGCCTTCATCGTCGTCCCAAGTGGACGAAATGTTGGACTCGTTCCTGGAAATTGATGATCAGAGCTTTGCATTGCCGCGGGTAACATCACTCCGAATGCTACAGCAAGAGGAGAAGTTCGGGTTTCACAACATGGGTGCTGGACTTTTCTCTGATTGGGTTAACCCGACAGATATTGAATCCTCACCGGAGTTTGGATTAGGAAACCAGAGTCAGGGGATGCTGAATTACGGTTGTAATGACTATTGTGTTCCTTCCGTGCCACCGTTTGGTAACATGGACTTGTCAGCGGAGTCCAAGAACCCCACTGACGAAGAGGTTCAAAGCGGAATGAGGACTAACCGGGTCACCGACCCTTCTAGTTTATATCAACATAATAATAATGTATTTTTATCGGGCTTGGGTGACCCATTTGGGTTTGGGTATTCGGGTCATTAA
Protein sequence

>MsaT043046.1

MEGRTPPLMWCTASTPRCLMHQSRWVYEGGRGSDSRGRKERKKKSKANFGEKEWYFFSPRDRKYPNGSRPNRVAGSGYWKATGTDKIITTEGRKVGIKKALVFYIGKAPKGTKTNWIMHEYRLLDASRKHNLGSTKLDDWVLCRIYKKNSSSQKEIPTFSTKECSNGSSPSSSSQVDEMLDSFLEIDDQSFALPRVTSLRMLQQEEKFGFHNMGAGLFSDWVNPTDIESSPEFGLGNQSQGMLNYGCNDYCVPSVPPFGNMDLSAESKNPTDEEVQSGMRTNRVTDPSSLYQHNNNVFLSGLGDPFGFGYSGH*