Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043295 | XP_024627831.1 | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.59e-57 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043295 | sp|F4JCN9|PRE4_ARATH | 69.474 | 95 | 24 | 3 | 1 | 93 | 1 | 92 | 6.45e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043295 | tr|A0A396GKL5|A0A396GKL5_MEDTR | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 7.62e-58 | 182 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG043295 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043295 | MtrunA17_Chr8g0362131 | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.47e-61 | 182 |
| MsaG043295 | MtrunA17_Chr5g0416431 | 82.051 | 78 | 13 | 1 | 16 | 92 | 16 | 93 | 1.76e-39 | 126 |
| MsaG043295 | MtrunA17_Chr7g0217141 | 75.532 | 94 | 21 | 2 | 1 | 93 | 1 | 93 | 2.08e-38 | 123 |
| MsaG043295 | MtrunA17_Chr6g0453041 | 74.359 | 78 | 19 | 1 | 17 | 93 | 15 | 92 | 3.15e-32 | 107 |
| MsaG043295 | MtrunA17_Chr3g0093001 | 71.795 | 78 | 19 | 2 | 17 | 93 | 17 | 92 | 4.77e-31 | 104 |
| MsaG043295 | MtrunA17_Chr7g0237771 | 50.617 | 81 | 34 | 3 | 1 | 80 | 1 | 76 | 4.47e-13 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043295 | AT3G47710.1 | 69.474 | 95 | 24 | 3 | 1 | 93 | 1 | 92 | 6.55e-37 | 120 |
| MsaG043295 | AT3G28857.1 | 65.957 | 94 | 28 | 2 | 1 | 92 | 1 | 92 | 8.25e-36 | 117 |
| MsaG043295 | AT1G26945.1 | 78.378 | 74 | 15 | 1 | 19 | 91 | 19 | 92 | 1.31e-33 | 111 |
| MsaG043295 | AT5G15160.1 | 68.000 | 75 | 22 | 1 | 19 | 91 | 18 | 92 | 1.54e-31 | 106 |
| MsaG043295 | AT1G74500.1 | 73.418 | 79 | 17 | 2 | 17 | 93 | 17 | 93 | 1.94e-31 | 106 |
| MsaG043295 | AT5G39860.1 | 66.667 | 78 | 24 | 1 | 17 | 92 | 15 | 92 | 8.37e-31 | 104 |
Find 14 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACCGATTGTCACAACTTT+TGG | 0.247784 | 8:-42819813 | MsaT043295.1:CDS |
| TTGAGCACTGTTAGGGTCTA+TGG | 0.329793 | 8:+42819787 | None:intergenic |
| ATAGCTGCTTGAGCACTGTT+AGG | 0.352323 | 8:+42819779 | None:intergenic |
| TGGGAACGCCTTGAGAGTTC+AGG | 0.406573 | 8:+42820902 | None:intergenic |
| AGAAAATTATTATACCTTAT+GGG | 0.418461 | 8:+42820883 | None:intergenic |
| GTGCTCAAGCAGCTATCATT+AGG | 0.423047 | 8:-42819772 | MsaT043295.1:CDS |
| ATTGTAGGTTTCAGCTGCTA+AGG | 0.479345 | 8:-42819894 | MsaT043295.1:intron |
| AGATCTCGTTCAAGACAATC+TGG | 0.480776 | 8:-42820989 | MsaT043295.1:CDS |
| AGGTACAAGTTGTTGTAACT+TGG | 0.482816 | 8:+42820922 | None:intergenic |
| ACTCTCAAGGCGTTCCCATA+AGG | 0.483554 | 8:-42820897 | MsaT043295.1:intron |
| TAGCTGCTTGAGCACTGTTA+GGG | 0.522205 | 8:+42819780 | None:intergenic |
| TGTCCAAAAGTTGTGACAAT+CGG | 0.545364 | 8:+42819810 | None:intergenic |
| AACTTGTACCTGAACTCTCA+AGG | 0.607839 | 8:-42820910 | MsaT043295.1:CDS |
| TTGAGCATCAGAGATCTCAG+TGG | 0.738554 | 8:+42820961 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 42819754 | 42821023 | 42819754 | ID=MsaG043295 |
| Chr8 | mRNA | 42819754 | 42821023 | 42819754 | ID=MsaT043295.1;Parent=MsaG043295 |
| Chr8 | exon | 42819754 | 42819909 | 42819754 | ID=MsaT043295.1.exon2;Parent=MsaT043295.1 |
| Chr8 | CDS | 42819754 | 42819909 | 42819754 | ID=cds.MsaT043295.1;Parent=MsaT043295.1 |
| Chr8 | exon | 42820898 | 42821023 | 42820898 | ID=MsaT043295.1.exon1;Parent=MsaT043295.1 |
| Chr8 | CDS | 42820898 | 42821023 | 42820898 | ID=cds.MsaT043295.1;Parent=MsaT043295.1 |
| Gene Sequence |
| Protein sequence |
