AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043297


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043297 MtrunA17_Chr8g0362151 100.000 282 0 0 1 282 1 282 0.0 586
MsaG043297 MtrunA17_Chr5g0416391 81.818 286 42 6 1 282 4 283 1.33e-150 422
MsaG043297 MtrunA17_Chr6g0453021 50.498 301 98 10 1 279 1 272 1.69e-84 254
MsaG043297 MtrunA17_Chr7g0217111 52.465 284 113 9 1 275 1 271 3.95e-82 248
MsaG043297 MtrunA17_Chr3g0123691 51.656 151 60 4 33 180 26 166 3.66e-39 139
MsaG043297 MtrunA17_Chr6g0450991 64.948 97 34 0 76 172 66 162 6.53e-39 137
MsaG043297 MtrunA17_Chr3g0119291 49.689 161 62 3 64 213 46 198 1.03e-37 134
MsaG043297 MtrunA17_Chr5g0415941 66.667 96 32 0 77 172 87 182 4.74e-37 134
MsaG043297 MtrunA17_Chr4g0060031 58.475 118 46 2 52 169 73 187 9.47e-37 133
MsaG043297 MtrunA17_Chr8g0381741 62.245 98 37 0 75 172 52 149 2.19e-36 132
MsaG043297 MtrunA17_Chr8g0362751 65.625 96 33 0 77 172 89 184 3.70e-36 130
MsaG043297 MtrunA17_Chr7g0265521 45.652 138 59 2 44 172 21 151 5.79e-33 120
MsaG043297 MtrunA17_Chr1g0179581 56.522 92 38 1 65 156 18 107 1.03e-27 103
MsaG043297 MtrunA17_Chr3g0127511 53.846 91 42 0 79 169 40 130 3.88e-27 105
MsaG043297 MtrunA17_Chr8g0346251 38.000 150 82 3 84 222 48 197 4.85e-25 100
MsaG043297 MtrunA17_Chr4g0055851 40.496 121 69 1 43 163 89 206 5.94e-21 90.1
MsaG043297 MtrunA17_Chr5g0403691 46.809 94 50 0 77 170 53 146 2.30e-19 84.3
MsaG043297 MtrunA17_Chr5g0403701 47.778 90 47 0 77 166 53 142 2.34e-19 84.0
MsaG043297 MtrunA17_Chr5g0399491 41.463 123 70 2 43 163 100 222 7.10e-19 84.3
MsaG043297 MtrunA17_Chr4g0054421 45.263 95 52 0 69 163 119 213 8.27e-19 84.0
MsaG043297 MtrunA17_Chr7g0258831 46.939 98 48 1 77 174 160 253 3.29e-18 82.8
MsaG043297 MtrunA17_Chr5g0400781 44.000 100 56 0 69 168 115 214 3.74e-18 82.0
MsaG043297 MtrunA17_Chr1g0175651 42.609 115 62 1 60 174 137 247 4.34e-18 82.4
MsaG043297 MtrunA17_Chr2g0308611 39.669 121 69 1 54 174 177 293 1.64e-17 81.3
MsaG043297 MtrunA17_Chr8g0335131 46.739 92 49 0 77 168 146 237 2.28e-17 80.5
MsaG043297 MtrunA17_Chr4g0071411 45.882 85 46 0 80 164 91 175 6.05e-17 77.8
MsaG043297 MtrunA17_Chr1g0151971 45.455 88 48 0 77 164 130 217 1.11e-16 78.2
MsaG043297 MtrunA17_Chr4g0044091 43.434 99 52 1 77 175 65 159 2.40e-15 72.8
MsaG043297 MtrunA17_Chr3g0135331 48.611 72 37 0 77 148 145 216 4.26e-14 71.2
MsaG043297 MtrunA17_Chr2g0299031 37.615 109 64 2 59 163 60 168 9.34e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043297 AT1G69780.1 64.189 296 84 8 1 282 7 294 8.58e-120 345
MsaG043297 AT1G26960.1 55.363 289 81 9 1 282 8 255 5.23e-95 281
MsaG043297 AT3G01220.1 48.057 283 114 9 1 275 16 273 1.07e-74 230
MsaG043297 AT5G15150.1 44.300 307 111 11 1 275 22 300 1.47e-71 223
MsaG043297 AT4G40060.1 57.576 132 52 2 59 186 39 170 4.71e-40 141
MsaG043297 AT5G65310.2 48.824 170 70 4 52 206 19 186 7.52e-40 140
MsaG043297 AT5G65310.1 48.824 170 70 4 52 206 37 204 8.71e-40 141
MsaG043297 AT3G01470.1 48.052 154 65 1 34 172 10 163 6.06e-39 137
MsaG043297 AT2G22430.1 66.667 99 33 0 74 172 59 157 8.64e-39 138
MsaG043297 AT1G27045.1 40.306 196 94 5 8 195 12 192 2.63e-31 116
MsaG043297 AT1G27045.4 42.857 161 75 4 8 166 12 157 1.64e-29 112
MsaG043297 AT1G27045.2 48.800 125 58 1 77 195 17 141 4.02e-28 107
MsaG043297 AT1G27045.5 48.800 125 58 1 77 195 25 149 4.60e-28 107
MsaG043297 AT1G27045.3 48.800 125 58 1 77 195 25 149 4.60e-28 107
MsaG043297 AT2G46680.1 52.688 93 44 0 78 170 33 125 8.27e-28 108
MsaG043297 AT2G46680.2 53.333 90 42 0 78 167 33 122 4.57e-27 106
MsaG043297 AT3G61890.1 51.111 90 44 0 80 169 33 122 1.28e-25 102
MsaG043297 AT5G03790.1 52.273 88 42 0 77 164 77 164 1.58e-24 99.0
MsaG043297 AT5G03790.2 55.000 80 36 0 77 156 77 156 1.49e-23 95.5
MsaG043297 AT2G18550.1 46.341 123 56 3 77 196 61 176 1.65e-22 93.2
MsaG043297 AT2G36610.1 43.590 117 51 3 58 166 59 168 1.95e-22 92.0
MsaG043297 AT4G36740.1 48.544 103 46 1 68 170 46 141 6.98e-22 91.3
MsaG043297 AT4G36740.2 48.544 103 46 1 68 170 47 142 7.50e-22 91.3
MsaG043297 AT5G66700.1 45.378 119 56 2 61 172 57 173 3.41e-21 89.7
MsaG043297 AT5G53980.1 44.554 101 56 0 72 172 6 106 1.04e-20 86.7
MsaG043297 AT2G01430.1 43.396 106 60 0 58 163 120 225 5.02e-18 82.0
MsaG043297 AT5G47370.1 42.708 96 55 0 69 164 122 217 2.26e-17 80.5
MsaG043297 AT4G37790.1 41.228 114 63 1 61 174 110 219 3.87e-17 79.7
MsaG043297 AT2G22800.1 46.667 90 48 0 75 164 111 200 4.95e-17 79.3
MsaG043297 AT5G06710.1 43.434 99 56 0 66 164 179 277 8.23e-17 79.7
MsaG043297 AT4G17460.1 41.414 99 58 0 65 163 123 221 1.18e-16 78.6
MsaG043297 AT3G60390.1 42.991 107 57 1 64 170 149 251 1.62e-16 78.6
MsaG043297 AT2G44910.1 44.681 94 48 1 77 170 163 252 5.28e-15 74.3
MsaG043297 AT4G16780.1 42.157 102 55 1 69 170 121 218 8.91e-15 73.2
MsaG043297 AT1G70920.1 37.903 124 69 2 57 180 50 165 9.54e-15 71.6
MsaG043297 AT1G70920.2 37.903 124 69 2 57 180 20 135 1.71e-14 70.5
MsaG043297 AT2G01430.2 50.704 71 35 0 59 129 121 191 2.56e-13 67.8

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTTCTGTTTCTTTATTA+AGG 0.139873 8:+42877751 None:intergenic
CATAGATCTCTTTCCTAAAA+AGG 0.254932 8:+42879021 None:intergenic
CTGAATGTCTTGTCTTGAAT+TGG 0.288821 8:+42877572 None:intergenic
AAGACAATATGATACTATTA+AGG 0.363112 8:-42878723 MsaT043297.1:CDS
GTTTGTAGCATGAAATTTGC+TGG 0.365160 8:+42879355 None:intergenic
TCCGATGATGGATCACAGTT+AGG 0.393627 8:-42878947 MsaT043297.1:CDS
GTCTTGAAGAAGATGGAAAT+AGG 0.399749 8:+42877625 None:intergenic
AGCCAAGACAGATTGCAATA+TGG 0.402938 8:-42878808 MsaT043297.1:CDS
ATGCAGCTAGCAAGAGCTCT+TGG 0.439885 8:-42878836 MsaT043297.1:CDS
TGGATGATCAATCTGGACTA+TGG 0.444127 8:-42877491 MsaT043297.1:CDS
CCATCTTCTTCAAGACCAGC+AGG 0.460199 8:-42877618 MsaT043297.1:CDS
CCGATGATGGATCACAGTTA+GGG 0.466027 8:-42878946 MsaT043297.1:CDS
CTTGTCTGTTGAGTAGATAA+AGG 0.476490 8:+42877654 None:intergenic
AGCTGATGAGGAACTCCTGC+TGG 0.491266 8:+42877603 None:intergenic
TGTGCCATGGATGATCAATC+TGG 0.494499 8:-42877498 MsaT043297.1:CDS
CAATATGGTTTCAAAACAGA+AGG 0.502644 8:-42878793 MsaT043297.1:CDS
ATAATTGAGTTGAGTGAAGG+TGG 0.507171 8:+42879304 None:intergenic
AACCATATTGCAATCTGTCT+TGG 0.512659 8:+42878806 None:intergenic
CCTGCTGGTCTTGAAGAAGA+TGG 0.521993 8:+42877618 None:intergenic
ATCTGGACTATGGCCATGGT+TGG 0.522424 8:-42877481 MsaT043297.1:CDS
TTAAGGTTGATTGATTCAGT+TGG 0.542418 8:+42877768 None:intergenic
CCCTAACTGTGATCCATCAT+CGG 0.548112 8:+42878946 None:intergenic
GAGGAAGAATTATCCGATGA+TGG 0.563562 8:-42878959 MsaT043297.1:CDS
GGTTTCAAAACAGAAGGGCT+AGG 0.568856 8:-42878787 MsaT043297.1:CDS
TAGTCCAGATTGATCATCCA+TGG 0.576768 8:+42877494 None:intergenic
TTGGTTTGAAAGAGCTGATG+AGG 0.592238 8:+42877591 None:intergenic
AACCTTAATAAAGAAACAGA+AGG 0.601474 8:-42877753 MsaT043297.1:CDS
GTTATAATTGAGTTGAGTGA+AGG 0.601931 8:+42879301 None:intergenic
AGTTAGGGGAGAAGAAGAGA+AGG 0.602528 8:-42878931 MsaT043297.1:CDS
AAGTGTTGTTGCTCCAACCA+TGG 0.605144 8:+42877468 None:intergenic
GAAGAAGAGAAGGCTTAACA+TGG 0.612268 8:-42878921 MsaT043297.1:CDS
ATCAATCTGGACTATGGCCA+TGG 0.615676 8:-42877485 MsaT043297.1:CDS
AATATGGTTTCAAAACAGAA+GGG 0.618611 8:-42878792 MsaT043297.1:CDS
ATTGAGTTGAGTGAAGGTGG+AGG 0.620969 8:+42879307 None:intergenic
AAACAGTTCAGATATCAAGC+TGG 0.622724 8:-42877709 MsaT043297.1:CDS
GTAATCTTGAGGTGCACATG+AGG 0.639812 8:+42879279 None:intergenic
GAAACAAGCTTGAACCTGAG+AGG 0.652955 8:-42878862 MsaT043297.1:CDS
AAAGTACCATGGTAATCTTG+AGG 0.656226 8:+42879268 None:intergenic
TGGAGAAGAAGCGAATGTCG+AGG 0.659383 8:-42878978 MsaT043297.1:CDS
CGATGATGGATCACAGTTAG+GGG 0.661316 8:-42878945 MsaT043297.1:CDS
TTCAAAACAGAAGGGCTAGG+TGG 0.663747 8:-42878784 MsaT043297.1:CDS
TGTGCACCTCAAGATTACCA+TGG 0.714676 8:-42879274 MsaT043297.1:intron
TCAAGCTGGATATTTCAACA+AGG 0.727961 8:-42877695 MsaT043297.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 42877461 42879389 42877461 ID=MsaG043297
Chr8 mRNA 42877461 42879389 42877461 ID=MsaT043297.1;Parent=MsaG043297
Chr8 exon 42877461 42877814 42877461 ID=MsaT043297.1.exon3;Parent=MsaT043297.1
Chr8 CDS 42877461 42877814 42877461 ID=cds.MsaT043297.1;Parent=MsaT043297.1
Chr8 exon 42878676 42879055 42878676 ID=MsaT043297.1.exon2;Parent=MsaT043297.1
Chr8 CDS 42878676 42879055 42878676 ID=cds.MsaT043297.1;Parent=MsaT043297.1
Chr8 exon 42879275 42879389 42879275 ID=MsaT043297.1.exon1;Parent=MsaT043297.1
Chr8 CDS 42879275 42879389 42879275 ID=cds.MsaT043297.1;Parent=MsaT043297.1
Gene Sequence

>MsaT043297.1

ATGGCGTTCTTTCCAGCAAATTTCATGCTACAAACTTCTCATCAAGATGAACATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGTGCACCTCAAGATTACCATGGAGGAGGAGTATCCTTTTTAGGAAAGAGATCTATGTCATTTTCAGGGATAGAACTTGGAGAAGAAGCGAATGTCGAGGAAGAATTATCCGATGATGGATCACAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACACTTGAGAAGAGTTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTAGCAAGAGCTCTTGGATTGCAGCCAAGACAGATTGCAATATGGTTTCAAAACAGAAGGGCTAGGTGGAAGACAAAACAGTTAGAGAAAGACTATGATGTTCTCAAAAGACAATATGATACTATTAAGGCTGATAATGATGCACTTCAAGCTCAGAATCAAAAACTTCAGACTGAGATATTGGCACTGAAAAATAGAGAACCAACTGAATCAATCAACCTTAATAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGATATCAAGCTGGATATTTCAACAAGGACACAAGCTATTGATAGTCCTTTATCTACTCAACAGACAAGCATAAACCTATTTCCATCTTCTTCAAGACCAGCAGGAGTTCCTCATCAGCTCTTTCAAACCAATTCAAGACAAGACATTCAGTGTCAAAAAATTGATCACATGGTTAAGGAAGAGAGCTTGAGTAACATGTTTTGTGCCATGGATGATCAATCTGGACTATGGCCATGGTTGGAGCAACAACACTTCAATTAA

Protein sequence

>MsaT043297.1

MAFFPANFMLQTSHQDEHHQPPPSLNSIITSCAPQDYHGGGVSFLGKRSMSFSGIELGEEANVEEELSDDGSQLGEKKRRLNMEQVKTLEKSFELGNKLEPERKMQLARALGLQPRQIAIWFQNRRARWKTKQLEKDYDVLKRQYDTIKADNDALQAQNQKLQTEILALKNREPTESINLNKETEGSSSNRSENSSDIKLDISTRTQAIDSPLSTQQTSINLFPSSSRPAGVPHQLFQTNSRQDIQCQKIDHMVKEESLSNMFCAMDDQSGLWPWLEQQHFN*