AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043398

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043398 MtrunA17_Chr2g0309121 90.164 122 11 1 1 122 1 121 9.13e-76 220
MsaG043398 MtrunA17_Chr2g0281511 84.426 122 19 0 1 122 1 122 1.33e-72 212
MsaG043398 MtrunA17_Chr3g0081891 83.750 80 13 0 1 80 1 80 8.78e-44 137
MsaG043398 MtrunA17_Chr7g0276661 32.061 131 69 4 7 119 38 166 3.66e-16 70.5
MsaG043398 MtrunA17_Chr0c01g0489371 42.857 91 50 1 22 110 224 314 8.78e-16 71.6
MsaG043398 MtrunA17_Chr8g0356961 41.573 89 50 1 24 110 77 165 4.30e-15 67.8
MsaG043398 MtrunA17_Chr8g0372431 27.835 97 70 0 24 120 6 102 1.17e-13 62.0
MsaG043398 MtrunA17_Chr5g0434641 36.264 91 56 1 22 110 208 298 1.31e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGTTATTGCTTCTATTT+TGG 0.132946 8:-45214227 MsaT043398.1:CDS
GAAGTCAATGGTACTGAATT+AGG 0.159497 8:-45214286 MsaT043398.1:CDS
TGTATGCTACCGTCGTTAAC+TGG 0.211996 8:-45213867 MsaT043398.1:CDS
AAAATATCGATGTCCATTAT+TGG 0.234776 8:-45214255 MsaT043398.1:CDS
TACCGGTTTCTTCATCAAAA+AGG 0.272684 8:+45214034 None:intergenic
TAACCTTATGCTTCCAATAA+TGG 0.277357 8:+45214242 None:intergenic
ATCAAAGTCATAAGATTTAC+CGG 0.326757 8:+45214017 None:intergenic
CCTCGATGTGTTGCGGAGAA+TGG 0.338723 8:-45214084 MsaT043398.1:CDS
TTTGGATGGAAACAATGTTT+TGG 0.356448 8:-45214209 MsaT043398.1:intron
TAAGGAAACATGAATGAGAT+TGG 0.380138 8:-45214313 None:intergenic
GTACAACTTTGTTCTTGAAA+AGG 0.417029 8:-45213944 MsaT043398.1:CDS
AAGTCAATGGTACTGAATTA+GGG 0.437113 8:-45214285 MsaT043398.1:CDS
TGTCCATTATTGGAAGCATA+AGG 0.460083 8:-45214245 MsaT043398.1:CDS
CGGTCAATAATCCTCCTAAT+CGG 0.465785 8:-45213891 MsaT043398.1:CDS
ACGGTAGCATACATCCGATT+AGG 0.477261 8:+45213877 None:intergenic
CTTGAAAAGGACCTAAAAGT+TGG 0.486000 8:-45213931 MsaT043398.1:CDS
AGAAATACATAGGGAGAGAA+TGG 0.518052 8:-45213966 MsaT043398.1:CDS
GTATGCTACCGTCGTTAACT+GGG 0.522932 8:-45213866 MsaT043398.1:CDS
AACATGCATCAGAAATACAT+AGG 0.535430 8:-45213976 MsaT043398.1:CDS
GAGATTGGAGTTGAAGTCAA+TGG 0.575541 8:-45214298 MsaT043398.1:CDS
ACATGCATCAGAAATACATA+GGG 0.587826 8:-45213975 MsaT043398.1:CDS
GTAGCATACATCCGATTAGG+AGG 0.600441 8:+45213880 None:intergenic
CCATTCTCCGCAACACATCG+AGG 0.699602 8:+45214084 None:intergenic
GAATCAGTCCCAGTTAACGA+CGG 0.733495 8:+45213858 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 45213861 45214326 45213861 ID=MsaG043398
Chr8 mRNA 45213861 45214326 45213861 ID=MsaT043398.1;Parent=MsaG043398
Chr8 exon 45213861 45214112 45213861 ID=MsaT043398.1.exon2;Parent=MsaT043398.1
Chr8 CDS 45213861 45214112 45213861 ID=cds.MsaT043398.1;Parent=MsaT043398.1
Chr8 exon 45214210 45214326 45214210 ID=MsaT043398.1.exon1;Parent=MsaT043398.1
Chr8 CDS 45214210 45214326 45214210 ID=cds.MsaT043398.1;Parent=MsaT043398.1
Gene Sequence

>MsaT043398.1

ATGAATGAGATTGGAGTTGAAGTCAATGGTACTGAATTAGGGAATGTTCAAAATATCGATGTCCATTATTGGAAGCATAAGGTTATTGCTTCTATTTTGGATGGAAACAATGTTTTGCATTTTCCTCGATGTGTTGCGGAGAATGGTTTGTTTATCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACCGGTAAATCTTATGACTTTGATGTTCACACTACGTCAAAAAACATGCATCAGAAATACATAGGGAGAGAATGGTACAACTTTGTTCTTGAAAAGGACCTAAAAGTTGGTGACGTTATTTTGTTCACGGTCAATAATCCTCCTAATCGGATGTATGCTACCGTCGTTAACTGGGACTGA
Protein sequence

>MsaT043398.1

MNEIGVEVNGTELGNVQNIDVHYWKHKVIASILDGNNVLHFPRCVAENGLFINQSKILLFDEETGKSYDFDVHTTSKNMHQKYIGREWYNFVLEKDLKVGDVILFTVNNPPNRMYATVVNWD*