Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043590 | XP_003628661.1 | 94.059 | 101 | 6 | 0 | 39 | 139 | 82 | 182 | 7.78e-62 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043590 | sp|Q9XIK0|PRIN2_ARATH | 51.546 | 97 | 44 | 1 | 39 | 135 | 83 | 176 | 4.80e-28 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043590 | tr|G7LFF8|G7LFF8_MEDTR | 94.059 | 101 | 6 | 0 | 39 | 139 | 82 | 182 | 3.71e-62 | 197 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG043590 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043590 | MtrunA17_Chr8g0364581 | 94.059 | 101 | 6 | 0 | 39 | 139 | 82 | 182 | 7.15e-66 | 197 |
| MsaG043590 | MtrunA17_Chr8g0364551 | 53.763 | 93 | 40 | 1 | 42 | 134 | 76 | 165 | 1.06e-24 | 93.2 |
| MsaG043590 | MtrunA17_Chr4g0063361 | 57.895 | 38 | 16 | 0 | 1 | 38 | 202 | 239 | 2.42e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043590 | AT1G10522.1 | 51.546 | 97 | 44 | 1 | 39 | 135 | 83 | 176 | 4.88e-29 | 104 |
| MsaG043590 | AT1G10522.2 | 51.546 | 97 | 44 | 1 | 39 | 135 | 83 | 176 | 4.88e-29 | 104 |
Find 35 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATGAAGATTGGGAAATT+TGG | 0.189011 | 8:-48211746 | MsaT043590.1:CDS |
| CAGCCAGAGCATCACTATTA+TGG | 0.234691 | 8:-48211782 | MsaT043590.1:CDS |
| ATACAGGAGGCTGTCCCTTT+AGG | 0.257782 | 8:-48218492 | MsaT043590.1:CDS |
| TACAGGAGGCTGTCCCTTTA+GGG | 0.297579 | 8:-48218491 | MsaT043590.1:CDS |
| AAAATTATGTTGATGAAGAT+TGG | 0.320502 | 8:-48211755 | MsaT043590.1:CDS |
| CTGCCACTTGAGACATAGTT+TGG | 0.355821 | 8:+48218516 | None:intergenic |
| TGAACTGTTACAGAAGCTTT+CGG | 0.394580 | 8:-48215708 | MsaT043590.1:CDS |
| CACAAGGAGTATGGAGGTCC+TGG | 0.413187 | 8:-48211879 | MsaT043590.1:CDS |
| CTGGCTGCCAGAGGTGCTCC+TGG | 0.416650 | 8:+48211798 | None:intergenic |
| TCGGAGGATGTAGATGAGTT+TGG | 0.433916 | 8:-48215683 | MsaT043590.1:CDS |
| AAATTATGTTGATGAAGATT+GGG | 0.435604 | 8:-48211754 | MsaT043590.1:CDS |
| AGACAGCAAAGGGCACCAAC+TGG | 0.444279 | 8:-48218543 | MsaT043590.1:CDS |
| AGCCAGAGCATCACTATTAT+GGG | 0.450002 | 8:-48211781 | MsaT043590.1:CDS |
| TGCCCATAATAGTGATGCTC+TGG | 0.457111 | 8:+48211779 | None:intergenic |
| GAAATTACCAGGAGCACCTC+TGG | 0.465147 | 8:-48211805 | MsaT043590.1:CDS |
| AAGATACATTCCTCCCTAAA+GGG | 0.467243 | 8:+48218478 | None:intergenic |
| AAAGATACATTCCTCCCTAA+AGG | 0.492765 | 8:+48218477 | None:intergenic |
| GTTACAGAAGCTTTCGGACT+CGG | 0.498546 | 8:-48215702 | MsaT043590.1:CDS |
| ACATAGTTTGGTCTTCCAGT+TGG | 0.499460 | 8:+48218528 | None:intergenic |
| TGTCTCAAGTGGCAGCATAC+AGG | 0.520249 | 8:-48218508 | MsaT043590.1:CDS |
| AGTTGTTGACGCTTGTGCTC+AGG | 0.524423 | 8:-48215645 | MsaT043590.1:intron |
| GATGTGAACAAGACAGCAAA+GGG | 0.529957 | 8:-48218553 | MsaT043590.1:CDS |
| TGTATCTTTGCTCATGGAGG+AGG | 0.531389 | 8:-48218463 | MsaT043590.1:intron |
| GAGGAATGTATCTTTGCTCA+TGG | 0.536789 | 8:-48218469 | MsaT043590.1:CDS |
| GGTGTCACCAGCAGTGTCCC+AGG | 0.546294 | 8:+48211861 | None:intergenic |
| TGGAGGTCCTGGGACACTGC+TGG | 0.563733 | 8:-48211868 | MsaT043590.1:CDS |
| GGATGTGAACAAGACAGCAA+AGG | 0.618693 | 8:-48218554 | MsaT043590.1:CDS |
| CTCAAGTGGCAGCATACAGG+AGG | 0.631604 | 8:-48218505 | MsaT043590.1:CDS |
| AGACCAAACTATGTCTCAAG+TGG | 0.636729 | 8:-48218519 | MsaT043590.1:CDS |
| ACAAGGAGTATGGAGGTCCT+GGG | 0.638608 | 8:-48211878 | MsaT043590.1:CDS |
| GCTACAAACATGTCTGTGAA+CGG | 0.655115 | 8:+48211840 | None:intergenic |
| ACAGAAGCTTTCGGACTCGG+AGG | 0.668368 | 8:-48215699 | MsaT043590.1:CDS |
| AGTGATGCTCTGGCTGCCAG+AGG | 0.670651 | 8:+48211789 | None:intergenic |
| AGGAGGCTGTCCCTTTAGGG+AGG | 0.673182 | 8:-48218488 | MsaT043590.1:CDS |
| GAATGTATCTTTGCTCATGG+AGG | 0.708939 | 8:-48218466 | MsaT043590.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 48211728 | 48218581 | 48211728 | ID=MsaG043590 |
| Chr8 | mRNA | 48211728 | 48218581 | 48211728 | ID=MsaT043590.1;Parent=MsaG043590 |
| Chr8 | exon | 48211728 | 48211919 | 48211728 | ID=MsaT043590.1.exon3;Parent=MsaT043590.1 |
| Chr8 | CDS | 48211728 | 48211919 | 48211728 | ID=cds.MsaT043590.1;Parent=MsaT043590.1 |
| Chr8 | exon | 48215646 | 48215755 | 48215646 | ID=MsaT043590.1.exon2;Parent=MsaT043590.1 |
| Chr8 | CDS | 48215646 | 48215755 | 48215646 | ID=cds.MsaT043590.1;Parent=MsaT043590.1 |
| Chr8 | exon | 48218464 | 48218581 | 48218464 | ID=MsaT043590.1.exon1;Parent=MsaT043590.1 |
| Chr8 | CDS | 48218464 | 48218581 | 48218464 | ID=cds.MsaT043590.1;Parent=MsaT043590.1 |
| Gene Sequence |
| Protein sequence |
