AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043709

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043709 MtrunA17_Chr8g0365941 96.795 468 14 1 1 468 1 467 0.0 927
MsaG043709 MtrunA17_Chr5g0421111 57.685 527 147 12 1 460 1 518 5.96e-177 507
MsaG043709 MtrunA17_Chr1g0152131 41.270 315 145 7 115 421 92 374 1.10e-57 196
MsaG043709 MtrunA17_Chr7g0234761 39.189 370 185 13 126 468 128 484 2.97e-51 181
MsaG043709 MtrunA17_Chr3g0144641 44.151 265 113 8 135 393 43 278 1.07e-50 177
MsaG043709 MtrunA17_Chr8g0339091 38.710 341 156 15 127 431 93 416 2.54e-48 173
MsaG043709 MtrunA17_Chr4g0035101 71.963 107 28 1 289 393 124 230 9.38e-46 162
MsaG043709 MtrunA17_Chr8g0363921 51.176 170 63 4 240 395 77 240 1.73e-41 149
MsaG043709 MtrunA17_Chr3g0131771 85.366 82 12 0 297 378 138 219 6.50e-41 149
MsaG043709 MtrunA17_Chr5g0415291 63.115 122 43 2 284 405 91 210 1.86e-40 145
MsaG043709 MtrunA17_Chr8g0385911 82.353 85 14 1 302 385 141 225 2.15e-40 147
MsaG043709 MtrunA17_Chr7g0258161 73.034 89 24 0 303 391 170 258 1.54e-39 145
MsaG043709 MtrunA17_Chr1g0174161 72.093 86 24 0 303 388 157 242 9.07e-38 140
MsaG043709 MtrunA17_Chr6g0484271 59.459 111 43 1 277 385 124 234 2.90e-34 129
MsaG043709 MtrunA17_Chr4g0074161 62.651 83 30 1 289 371 189 270 8.70e-23 100
MsaG043709 MtrunA17_Chr1g0190731 68.182 66 20 1 306 371 193 257 2.92e-20 92.0
MsaG043709 MtrunA17_Chr4g0014771 55.385 65 29 0 306 370 118 182 1.68e-15 75.1
MsaG043709 MtrunA17_Chr4g0032951 58.462 65 27 0 306 370 355 419 1.95e-15 78.2
MsaG043709 MtrunA17_Chr2g0329041 50.000 74 37 0 306 379 332 405 5.22e-15 76.6
MsaG043709 MtrunA17_Chr1g0174271 52.174 69 33 0 306 374 298 366 2.20e-14 74.7
MsaG043709 MtrunA17_Chr1g0193321 33.125 160 82 5 244 402 415 550 2.42e-14 75.5
MsaG043709 MtrunA17_Chr7g0258181 50.725 69 34 0 306 374 243 311 2.62e-14 73.9
MsaG043709 MtrunA17_Chr1g0182791 54.688 64 28 1 308 371 319 381 3.18e-14 74.7
MsaG043709 MtrunA17_Chr1g0184841 57.692 78 32 1 294 371 108 184 3.45e-14 72.8
MsaG043709 MtrunA17_Chr3g0087771 50.769 65 32 0 306 370 322 386 6.26e-13 70.5
MsaG043709 MtrunA17_Chr1g0153241 47.692 65 33 1 308 372 87 150 3.78e-12 66.6
MsaG043709 MtrunA17_Chr8g0351881 47.692 65 34 0 306 370 316 380 6.13e-12 67.0
MsaG043709 MtrunA17_Chr1g0152651 50.769 65 31 1 306 370 274 337 1.48e-11 65.9
MsaG043709 MtrunA17_Chr3g0095391 50.000 64 31 1 308 371 311 373 2.82e-11 65.5
MsaG043709 MtrunA17_Chr7g0262691 48.438 64 32 1 308 371 254 316 3.30e-11 65.1
MsaG043709 MtrunA17_Chr5g0402031 53.571 56 25 1 317 372 116 170 4.53e-11 64.3
MsaG043709 MtrunA17_Chr7g0231461 50.000 64 31 1 306 369 297 359 7.24e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043709 AT1G68920.3 44.812 453 196 16 17 455 28 440 2.61e-94 294
MsaG043709 AT1G68920.1 44.812 453 197 16 17 455 28 441 3.29e-94 293
MsaG043709 AT1G68920.4 44.812 453 197 16 17 455 28 441 3.29e-94 293
MsaG043709 AT1G68920.2 44.812 453 197 16 17 455 28 441 3.29e-94 293
MsaG043709 AT1G26260.4 40.189 423 165 13 25 441 13 353 6.58e-69 225
MsaG043709 AT1G26260.1 40.189 423 165 13 25 441 13 353 6.58e-69 225
MsaG043709 AT1G26260.6 40.189 423 165 13 25 441 13 353 6.58e-69 225
MsaG043709 AT1G26260.2 40.189 423 165 13 25 441 13 353 6.58e-69 225
MsaG043709 AT1G26260.5 46.006 313 122 9 135 441 60 331 6.21e-65 214
MsaG043709 AT1G26260.3 51.709 234 86 4 208 441 96 302 4.95e-63 208
MsaG043709 AT1G10120.2 51.562 192 83 2 214 405 124 305 4.47e-53 182
MsaG043709 AT1G10120.1 51.562 192 83 2 214 405 124 305 4.47e-53 182
MsaG043709 AT3G07340.2 42.910 268 98 8 127 393 132 345 8.55e-50 176
MsaG043709 AT3G07340.1 42.910 268 98 8 127 393 132 345 1.37e-49 176
MsaG043709 AT3G23690.1 48.947 190 70 6 272 438 152 337 1.88e-45 162
MsaG043709 AT5G48560.2 40.370 270 130 8 127 393 148 389 5.91e-45 163
MsaG043709 AT4G34530.1 68.750 112 30 2 303 409 169 280 6.54e-45 160
MsaG043709 AT5G48560.1 40.370 270 130 8 127 393 148 389 1.28e-44 163
MsaG043709 AT2G18300.1 54.110 146 62 1 240 385 124 264 2.59e-42 153
MsaG043709 AT5G50915.3 41.860 258 102 9 159 414 33 244 2.83e-42 151
MsaG043709 AT5G50915.4 41.860 258 102 9 159 414 33 244 2.83e-42 151
MsaG043709 AT2G18300.2 54.110 146 62 1 240 385 124 264 2.92e-42 153
MsaG043709 AT5G50915.1 40.071 282 115 11 159 437 33 263 4.62e-42 151
MsaG043709 AT5G50915.2 40.071 282 115 11 159 437 33 263 4.62e-42 151
MsaG043709 AT2G18300.3 54.110 146 62 1 240 385 124 264 6.35e-42 153
MsaG043709 AT1G59640.1 42.000 200 110 2 197 395 31 225 3.71e-41 148
MsaG043709 AT1G59640.2 41.919 198 109 2 197 393 31 223 7.07e-41 149
MsaG043709 AT4G36540.1 51.974 152 64 2 269 411 101 252 1.90e-40 147
MsaG043709 AT4G36540.2 59.200 125 48 1 269 390 101 225 2.54e-39 144
MsaG043709 AT2G42300.4 67.778 90 29 0 303 392 182 271 1.40e-38 141
MsaG043709 AT3G57800.2 73.256 86 23 0 303 388 201 286 1.24e-37 142
MsaG043709 AT2G42300.1 68.539 89 28 0 303 391 182 270 2.36e-37 139
MsaG043709 AT5G62610.2 74.074 81 20 1 297 377 145 224 1.01e-35 134
MsaG043709 AT5G62610.1 74.074 81 20 1 297 377 145 224 1.01e-35 134
MsaG043709 AT1G73830.2 61.321 106 41 0 280 385 121 226 1.74e-34 130
MsaG043709 AT1G73830.1 61.321 106 41 0 280 385 121 226 1.86e-34 130
MsaG043709 AT1G18400.2 62.264 106 35 2 304 407 143 245 2.06e-34 129
MsaG043709 AT1G18400.1 74.390 82 21 0 304 385 143 224 3.31e-34 129
MsaG043709 AT1G25330.1 68.132 91 29 0 304 394 102 192 8.48e-34 127
MsaG043709 AT3G57800.1 47.368 133 23 1 303 388 201 333 4.85e-29 119
MsaG043709 AT2G24260.2 59.091 88 33 2 284 371 15 99 6.52e-23 97.8
MsaG043709 AT2G24260.1 59.091 88 33 2 284 371 118 202 5.58e-22 97.4
MsaG043709 AT4G30980.1 57.143 98 36 3 274 371 103 194 1.10e-21 95.9
MsaG043709 AT4G30980.2 57.143 98 36 3 274 371 151 242 2.33e-21 95.5
MsaG043709 AT5G58010.1 70.149 67 19 1 305 371 98 163 3.66e-21 94.0
MsaG043709 AT2G42300.5 81.395 43 8 0 303 345 182 224 4.06e-17 80.9
MsaG043709 AT2G42300.3 81.395 43 8 0 303 345 182 224 4.06e-17 80.9
MsaG043709 AT2G42300.2 81.395 43 8 0 303 345 182 224 4.85e-17 80.9
MsaG043709 AT2G20180.4 38.393 112 57 3 262 371 241 342 2.02e-15 79.0
MsaG043709 AT2G20180.6 38.393 112 57 3 262 371 241 342 2.02e-15 79.0
MsaG043709 AT2G20180.2 38.393 112 57 3 262 371 241 342 2.02e-15 79.0
MsaG043709 AT2G20180.3 38.393 112 57 3 262 371 241 342 2.02e-15 79.0
MsaG043709 AT2G20180.7 38.393 112 57 3 262 371 170 271 2.26e-15 78.2
MsaG043709 AT2G20180.1 38.393 112 57 3 262 371 170 271 2.26e-15 78.2
MsaG043709 AT2G20180.5 38.393 112 57 3 262 371 170 271 2.26e-15 78.2
MsaG043709 AT1G51140.1 56.250 64 28 0 306 369 304 367 1.88e-14 75.1
MsaG043709 AT4G28790.1 33.562 146 82 4 275 416 236 370 2.43e-14 75.1
MsaG043709 AT2G20180.8 37.615 109 56 3 262 368 241 339 2.85e-14 74.3
MsaG043709 AT4G28800.1 34.959 123 60 3 268 389 229 332 5.61e-14 74.3
MsaG043709 AT1G35460.1 52.308 65 31 0 306 370 181 245 6.19e-14 72.0
MsaG043709 AT4G09180.1 51.515 66 32 0 305 370 183 248 7.02e-14 72.0
MsaG043709 AT2G42280.1 51.562 64 31 0 306 369 279 342 1.00e-13 72.8
MsaG043709 AT1G09530.3 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT1G09530.2 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT1G09530.5 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT1G09530.1 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT1G09530.4 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT1G09530.6 51.562 64 30 1 308 371 339 401 1.07e-13 73.6
MsaG043709 AT4G28815.2 56.897 58 24 1 309 366 71 127 1.92e-13 70.1
MsaG043709 AT2G42280.3 53.030 66 29 1 306 369 279 344 2.03e-13 72.0
MsaG043709 AT4G02590.3 55.422 83 34 2 291 371 66 147 2.63e-13 70.1
MsaG043709 AT4G28800.2 34.375 128 59 3 268 389 241 349 3.06e-13 72.0
MsaG043709 AT4G02590.2 55.422 83 34 2 291 371 129 210 4.29e-13 70.5
MsaG043709 AT4G02590.1 55.422 83 34 2 291 371 129 210 4.29e-13 70.5
MsaG043709 AT4G28815.1 56.897 58 24 1 309 366 147 203 5.86e-13 70.1
MsaG043709 AT1G03040.2 62.121 66 24 1 306 371 139 203 6.87e-13 69.7
MsaG043709 AT1G03040.1 62.121 66 24 1 306 371 144 208 8.22e-13 69.3
MsaG043709 AT1G03040.3 45.455 110 52 3 269 371 100 208 1.44e-12 68.9
MsaG043709 AT2G46970.1 34.513 113 68 2 304 411 216 327 4.58e-12 68.2
MsaG043709 AT2G46970.2 34.513 113 68 2 304 411 185 296 5.08e-12 67.8
MsaG043709 AT4G00050.2 49.275 69 34 1 302 370 101 168 5.88e-12 67.0
MsaG043709 AT2G46970.3 34.513 113 68 2 304 411 194 305 5.89e-12 67.8
MsaG043709 AT1G27740.1 37.398 123 60 4 264 370 109 230 6.31e-12 66.2
MsaG043709 AT4G28790.2 34.951 103 62 2 275 373 236 337 8.27e-12 67.0
MsaG043709 AT4G00050.1 49.275 69 34 1 302 370 203 270 1.31e-11 66.6
MsaG043709 AT4G00050.3 49.275 69 34 1 302 370 203 270 1.54e-11 66.2
MsaG043709 AT2G43140.2 50.000 64 32 0 306 369 231 294 3.27e-11 64.7
MsaG043709 AT2G43140.1 50.000 64 32 0 306 369 134 197 3.50e-11 63.2
MsaG043709 AT4G33880.1 49.231 65 32 1 306 370 266 329 4.57e-11 64.7
MsaG043709 AT4G36930.1 50.820 61 29 1 312 372 197 256 6.95e-11 64.3

Find 127 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATATGGTCCTAATTTCT+TGG 0.233035 8:+50040735 MsaT043709.1:CDS
TGCAATGGATTAACCTGATT+TGG 0.278397 8:-50043245 None:intergenic
AGTGGAGAACCTTCTATTAA+AGG 0.279819 8:+50041261 MsaT043709.1:CDS
TATGAATCTACCATTGAATT+AGG 0.293515 8:-50040718 None:intergenic
GCTTCAACAACCAAGGCTTC+TGG 0.303821 8:+50041877 MsaT043709.1:CDS
CACTTGCACAATCCAACATT+TGG 0.316306 8:-50041242 None:intergenic
CCTTCATCTTGAGACATTAC+AGG 0.318475 8:-50041153 None:intergenic
GCTCTCACAGTTGTCCTTTG+TGG 0.319104 8:-50041830 None:intergenic
GACTAGAACCATACATCTTT+AGG 0.325629 8:-50042946 None:intergenic
TGCAAATTCATCTGTAGGTT+TGG 0.339897 8:+50040657 MsaT043709.1:CDS
AGTCACTTCTTCGATCATTC+TGG 0.342191 8:-50041128 None:intergenic
ACCGGGTCCATCATCTGCTC+TGG 0.343461 8:+50043064 MsaT043709.1:CDS
GGATTTAGACCTTTAATAGA+AGG 0.344206 8:-50041270 None:intergenic
GTAAGAAGGTCCAAGAAATT+AGG 0.353442 8:-50040745 None:intergenic
GAAAGGAGGGTCCTTTAGAT+TGG 0.355470 8:+50040611 MsaT043709.1:CDS
TCAACCACAGTTGGAACCTT+TGG 0.357269 8:+50043205 MsaT043709.1:CDS
TCTGATAAATTTGCCATGTT+AGG 0.358693 8:-50043164 None:intergenic
TTCTGGGAAGAATGCCAAAC+AGG 0.361984 8:+50041894 MsaT043709.1:CDS
ACGAGGGATACGTACATGTT+AGG 0.367434 8:+50041941 MsaT043709.1:CDS
TCAGATTCTTCAACATCAAC+CGG 0.371564 8:+50043046 MsaT043709.1:intron
TCTTACTAACTCCGAAAACT+TGG 0.372119 8:+50040762 MsaT043709.1:CDS
GGCATTCTTCCCAGAAGCCT+TGG 0.393219 8:-50041887 None:intergenic
ATCTGTAGGTTTGGTTTCCA+TGG 0.397148 8:+50040666 MsaT043709.1:CDS
CTTCAACAACCAAGGCTTCT+GGG 0.404138 8:+50041878 MsaT043709.1:CDS
TTCCATGGAAAATTCTATGA+TGG 0.411759 8:+50040681 MsaT043709.1:CDS
GGTTGATATGAATGTGATAA+TGG 0.412278 8:-50043119 None:intergenic
GTGTGGGATTCCTCAATCTA+TGG 0.414177 8:+50041047 MsaT043709.1:CDS
CTTCTTCGATCATTCTGGAT+TGG 0.414720 8:-50041123 None:intergenic
GTTGAGGAAATTTCAGTCCT+TGG 0.419505 8:-50043273 None:intergenic
GGAAATATCTCAGCCCCATT+TGG 0.420071 8:-50040928 None:intergenic
CAGTCCTTGGCTGCTTGCAA+TGG 0.420935 8:-50043260 None:intergenic
TTCTTCATGTCCTAATTCAA+TGG 0.433965 8:+50040708 MsaT043709.1:CDS
CCAAAGAAAAGGAAAAGAAA+TGG 0.434507 8:+50041291 MsaT043709.1:CDS
GAAAACTGAGACAAACTTTG+AGG 0.438701 8:-50040949 None:intergenic
CCGGGTCCATCATCTGCTCT+GGG 0.439623 8:+50043065 MsaT043709.1:CDS
AGCAATGAAGTGACAAAAGA+TGG 0.443501 8:+50040811 MsaT043709.1:CDS
TTATTCTGTTTGTAGGTCAC+TGG 0.450051 8:+50042246 MsaT043709.1:intron
TCTCGGTCTGTTGAGCATCT+TGG 0.450146 8:+50041087 MsaT043709.1:CDS
GTTGGAATGGGTTGGGACCT+TGG 0.452583 8:+50040871 MsaT043709.1:CDS
ACTTGCACAATCCAACATTT+GGG 0.456863 8:-50041241 None:intergenic
CACTGGCAAGGCAGTGATGC+TGG 0.457604 8:+50042263 MsaT043709.1:CDS
ACTTGGTAGCTCAAGAGTTC+CGG 0.459094 8:-50041803 None:intergenic
TTTCTTCAAGATCTTGTACC+TGG 0.462998 8:+50042126 MsaT043709.1:CDS
AGGGTTTAAAGAGCCAAATC+AGG 0.463980 8:+50043232 MsaT043709.1:CDS
CAAATTTATCAGATATGCTT+AGG 0.466891 8:+50043174 MsaT043709.1:CDS
AGAAGCTGGAGACCCCTGTT+TGG 0.468557 8:-50041908 None:intergenic
GAAGTGACAAAAGATGGGTT+TGG 0.476292 8:+50040817 MsaT043709.1:CDS
CGAGGGATACGTACATGTTA+GGG 0.480619 8:+50041942 MsaT043709.1:CDS
AAGGTCTAAATCCAAAGAAA+AGG 0.482803 8:+50041280 MsaT043709.1:CDS
CTCTACCTCTACCTGTGTCT+CGG 0.483360 8:+50041070 MsaT043709.1:CDS
AATTCAATGGTAGATTCATA+TGG 0.486689 8:+50040721 MsaT043709.1:CDS
CCACAGTTGGAACCTTTGGC+TGG 0.487866 8:+50043209 MsaT043709.1:CDS
AGTTGGAACCTTTGGCTGGA+GGG 0.488222 8:+50043213 MsaT043709.1:CDS
AAATGTACCTTGTTGCATCC+AGG 0.496384 8:-50042144 None:intergenic
TAGAACAGTTGGAATGGGTT+GGG 0.502266 8:+50040864 MsaT043709.1:CDS
AGGAGGGTCCTTTAGATTGG+AGG 0.508576 8:+50040614 MsaT043709.1:CDS
TCCATGGAAAATTCTATGAT+GGG 0.513525 8:+50040682 MsaT043709.1:CDS
GAAAAGGAAAAGAAATGGAC+AGG 0.513621 8:+50041296 MsaT043709.1:CDS
TTGTCCTTTGTGGTTTCACT+TGG 0.513815 8:-50041820 None:intergenic
GGCTATTTGTTGCTTGACCA+CGG 0.515569 8:-50041969 None:intergenic
CGATGATGGTTCCCAAATGT+TGG 0.518846 8:+50041230 MsaT043709.1:CDS
CCTGTAATGTCTCAAGATGA+AGG 0.520207 8:+50041153 MsaT043709.1:CDS
TCGTTAGGCGGATCAGAAGC+TGG 0.521860 8:-50041922 None:intergenic
AGAAACCCCAGAGCAGATGA+TGG 0.522386 8:-50043071 None:intergenic
TTACTCTCTCTGCAAGGCTA+TGG 0.523831 8:-50041990 None:intergenic
GATGAAGGGAAACAAGCTCT+TGG 0.524819 8:+50041168 MsaT043709.1:CDS
CTCACAAACCTCCAATCTAA+AGG 0.533291 8:-50040622 None:intergenic
GCAGACTGTTCAACCACAGT+TGG 0.533598 8:+50043196 MsaT043709.1:CDS
TGATGATAGAACAGTTGGAA+TGG 0.544564 8:+50040858 MsaT043709.1:CDS
ATAGAACAGTTGGAATGGGT+TGG 0.546913 8:+50040863 MsaT043709.1:CDS
GAAGGGAAACAAGCTCTTGG+AGG 0.547800 8:+50041171 MsaT043709.1:CDS
TAATCCATTGCAAGCAGCCA+AGG 0.549245 8:+50043256 MsaT043709.1:CDS
ACAGACCGAGACACAGGTAG+AGG 0.549978 8:-50041075 None:intergenic
TCTTTAAACCCTCCAGCCAA+AGG 0.552371 8:-50043221 None:intergenic
AACCTTGCAAATTCATCTGT+AGG 0.552403 8:+50040652 MsaT043709.1:CDS
GGTTGTGATGATAGAACAGT+TGG 0.552863 8:+50040853 MsaT043709.1:CDS
CCCAGAGCAGATGATGGACC+CGG 0.555611 8:-50043065 None:intergenic
CAGTTGGAACCTTTGGCTGG+AGG 0.557432 8:+50043212 MsaT043709.1:CDS
AGAGGTAGAGCCATAGATTG+AGG 0.558788 8:-50041057 None:intergenic
CATCATATCCACGAAACTCC+CGG 0.562237 8:-50041019 None:intergenic
GATGATAGAACAGTTGGAAT+GGG 0.563426 8:+50040859 MsaT043709.1:CDS
GATGAATCGAGTGGCGATGA+TGG 0.566803 8:+50041216 MsaT043709.1:CDS
GTAGTAGTTCTATGATGAAG+AGG 0.567662 8:+50040893 MsaT043709.1:CDS
CTGTAATGTCTCAAGATGAA+GGG 0.576780 8:+50041154 MsaT043709.1:CDS
AGAGTTGAATGAATCAGTCC+TGG 0.580047 8:-50043140 None:intergenic
CTTACTAACTCCGAAAACTT+GGG 0.581398 8:+50040763 MsaT043709.1:CDS
CAAATGTTGGATTGTGCAAG+TGG 0.582909 8:+50041243 MsaT043709.1:CDS
ACTAACTCCGAAAACTTGGG+TGG 0.585659 8:+50040766 MsaT043709.1:CDS
AGCTTCTGATCCGCCTAACG+AGG 0.588431 8:+50041924 MsaT043709.1:CDS
GCAATGAAGTGACAAAAGAT+GGG 0.592265 8:+50040812 MsaT043709.1:CDS
TCTGGGAAGAATGCCAAACA+GGG 0.593622 8:+50041895 MsaT043709.1:CDS
ACATGTACGTATCCCTCGTT+AGG 0.594600 8:-50041937 None:intergenic
AAGGGAAACAAGCTCTTGGA+GGG 0.596219 8:+50041172 MsaT043709.1:CDS
TAGTAGTTCTATGATGAAGA+GGG 0.601347 8:+50040894 MsaT043709.1:CDS
CTGGATTTATTGATGCTGCA+AGG 0.604609 8:+50040980 MsaT043709.1:CDS
TTTGAGGTAGATAGAAAGGA+GGG 0.606702 8:+50040598 MsaT043709.1:CDS
CTGTTTGTAGGTCACTGGCA+AGG 0.607483 8:+50042251 MsaT043709.1:intron
TCCATATTAAAATCCACATG+TGG 0.609814 8:-50042913 None:intergenic
GATAATGGAGGAAAAGCCAT+TGG 0.610652 8:-50043104 None:intergenic
TCATTCAACTCTACCTAACA+TGG 0.611728 8:+50043151 MsaT043709.1:CDS
TGCTCAACAGACCGAGACAC+AGG 0.613918 8:-50041081 None:intergenic
AACCTACAGATGAATTTGCA+AGG 0.617947 8:-50040654 None:intergenic
CAGATTCTTCAACATCAACC+GGG 0.618225 8:+50043047 MsaT043709.1:intron
TGGTAGCTCAAGAGTTCCGG+TGG 0.619141 8:-50041800 None:intergenic
CCAGCCAAAGGTTCCAACTG+TGG 0.623573 8:-50043209 None:intergenic
GGGTCTTGTAATGAAGCTGA+TGG 0.623907 8:+50041192 MsaT043709.1:CDS
TGTACGTATCCCTCGTTAGG+CGG 0.628257 8:-50041934 None:intergenic
TCATCATAGAACTACTACCA+AGG 0.632663 8:-50040888 None:intergenic
TGGATATGATGAACTCGTGT+GGG 0.632999 8:+50041031 MsaT043709.1:CDS
AGTTCTATGATGAAGAGGGA+TGG 0.633166 8:+50040898 MsaT043709.1:CDS
GTCTCTTCAACAACAAGTTG+AGG 0.634721 8:+50042308 MsaT043709.1:CDS
TTAGATTGGAGGTTTGTGAG+TGG 0.643180 8:+50040625 MsaT043709.1:CDS
GGTGATAGTGATAAAGCCAC+CGG 0.644324 8:+50041784 MsaT043709.1:CDS
AAAATCTTACTCTCTCTGCA+AGG 0.646413 8:-50041996 None:intergenic
TGCAACAGTAAATCCACATG+TGG 0.646636 8:+50042900 MsaT043709.1:CDS
GCTTCTGATCCGCCTAACGA+GGG 0.648015 8:+50041925 MsaT043709.1:CDS
GCAAACAGCTTCAACAACCA+AGG 0.656445 8:+50041870 MsaT043709.1:CDS
TGATATGAATGTGATAATGG+AGG 0.657822 8:-50043116 None:intergenic
CGGGTCCATCATCTGCTCTG+GGG 0.660359 8:+50043066 MsaT043709.1:CDS
GTTTCTCAGAGAAATGCCAA+TGG 0.663274 8:+50043088 MsaT043709.1:CDS
GTGGATATGATGAACTCGTG+TGG 0.663347 8:+50041030 MsaT043709.1:CDS
TCTTGTACCTGGATGCAACA+AGG 0.668293 8:+50042137 MsaT043709.1:CDS
TTATCACATTCATATCAACC+AGG 0.710399 8:+50043122 MsaT043709.1:CDS
GCTACCAAGTGAAACCACAA+AGG 0.714013 8:+50041816 MsaT043709.1:CDS
CTGGGAAGAATGCCAAACAG+GGG 0.714387 8:+50041896 MsaT043709.1:CDS
GCTGATGGAGATGAATCGAG+TGG 0.745292 8:+50041207 MsaT043709.1:CDS
GTACATGTTAGGGCTCGCCG+TGG 0.768171 8:+50041952 MsaT043709.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 50040580 50043283 50040580 ID=MsaG043709
Chr8 mRNA 50040580 50043283 50040580 ID=MsaT043709.1;Parent=MsaG043709
Chr8 exon 50040580 50041317 50040580 ID=MsaT043709.1.exon1;Parent=MsaT043709.1
Chr8 CDS 50040580 50041317 50040580 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Chr8 exon 50041781 50042008 50041781 ID=MsaT043709.1.exon2;Parent=MsaT043709.1
Chr8 CDS 50041781 50042008 50041781 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Chr8 exon 50042093 50042158 50042093 ID=MsaT043709.1.exon3;Parent=MsaT043709.1
Chr8 CDS 50042093 50042158 50042093 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Chr8 exon 50042261 50042329 50042261 ID=MsaT043709.1.exon4;Parent=MsaT043709.1
Chr8 CDS 50042261 50042329 50042261 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Chr8 exon 50042883 50042954 50042883 ID=MsaT043709.1.exon5;Parent=MsaT043709.1
Chr8 CDS 50042883 50042954 50042883 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Chr8 exon 50043050 50043283 50043050 ID=MsaT043709.1.exon6;Parent=MsaT043709.1
Chr8 CDS 50043050 50043283 50043050 ID=cds.MsaT043709.1;Parent=MsaT043709.1
Gene Sequence

>MsaT043709.1

ATGAGTGAAAGTGAAAATTTTGAGGTAGATAGAAAGGAGGGTCCTTTAGATTGGAGGTTTGTGAGTGGCAACAACCTTGCAAATTCATCTGTAGGTTTGGTTTCCATGGAAAATTCTATGATGGGTTCTTCTTCATGTCCTAATTCAATGGTAGATTCATATGGTCCTAATTTCTTGGACCTTCTTACTAACTCCGAAAACTTGGGTGGTTTTTGTGATGTTAATGGTCAGAGCAATGAAGTGACAAAAGATGGGTTTGGTTTTGCAAGAGTTGGTTGTGATGATAGAACAGTTGGAATGGGTTGGGACCTTGGTAGTAGTTCTATGATGAAGAGGGATGGTGTTTTACCAAATGGGGCTGAGATATTTCCTCAAAGTTTGTCTCAGTTTTCAACTGATTCTGGATTTATTGATGCTGCAAGGATGTCATGTTTTAGTGCCGGGAGTTTCGTGGATATGATGAACTCGTGTGGGATTCCTCAATCTATGGCTCTACCTCTACCTGTGTCTCGGTCTGTTGAGCATCTTGGTAGTGATGAAAGTCCAATCCAGAATGATCGAAGAAGTGACTGTCCTGTAATGTCTCAAGATGAAGGGAAACAAGCTCTTGGAGGGTCTTGTAATGAAGCTGATGGAGATGAATCGAGTGGCGATGATGGTTCCCAAATGTTGGATTGTGCAAGTGGAGAACCTTCTATTAAAGGTCTAAATCCAAAGAAAAGGAAAAGAAATGGACAGGATGGTGATAGTGATAAAGCCACCGGAACTCTTGAGCTACCAAGTGAAACCACAAAGGACAACTGTGAGAGCAGAAAAAAGGGAAAGCAGCAAACAGCTTCAACAACCAAGGCTTCTGGGAAGAATGCCAAACAGGGGTCTCCAGCTTCTGATCCGCCTAACGAGGGATACGTACATGTTAGGGCTCGCCGTGGTCAAGCAACAAATAGCCATAGCCTTGCAGAGAGAGTGAGGAGAGAAAAGATCAGTGAAAGAATGAAGTTTCTTCAAGATCTTGTACCTGGATGCAACAAGGTCACTGGCAAGGCAGTGATGCTGGATGAAATCATTAATTATGTACAGTCTCTTCAACAACAAGTTGAGTTTTTATCAATGAAACTTGCAACAGTAAATCCACATGTGGATTTTAATATGGAACGGCTGCTTCCTAAAGATATTCTTCAACATCAACCGGGTCCATCATCTGCTCTGGGGTTTCTCAGAGAAATGCCAATGGCTTTTCCTCCATTATCACATTCATATCAACCAGGACTGATTCATTCAACTCTACCTAACATGGCAAATTTATCAGATATGCTTAGGCAGACTGTTCAACCACAGTTGGAACCTTTGGCTGGAGGGTTTAAAGAGCCAAATCAGGTTAATCCATTGCAAGCAGCCAAGGACTGA
Protein sequence

>MsaT043709.1

MSESENFEVDRKEGPLDWRFVSGNNLANSSVGLVSMENSMMGSSSCPNSMVDSYGPNFLDLLTNSENLGGFCDVNGQSNEVTKDGFGFARVGCDDRTVGMGWDLGSSSMMKRDGVLPNGAEIFPQSLSQFSTDSGFIDAARMSCFSAGSFVDMMNSCGIPQSMALPLPVSRSVEHLGSDESPIQNDRRSDCPVMSQDEGKQALGGSCNEADGDESSGDDGSQMLDCASGEPSIKGLNPKKRKRNGQDGDSDKATGTLELPSETTKDNCESRKKGKQQTASTTKASGKNAKQGSPASDPPNEGYVHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPHVDFNMERLLPKDILQHQPGPSSALGFLREMPMAFPPLSHSYQPGLIHSTLPNMANLSDMLRQTVQPQLEPLAGGFKEPNQVNPLQAAKD*