AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043783

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043783 MtrunA17_Chr8g0366581 99.149 235 2 0 1 235 1 235 2.90e-178 488
MsaG043783 MtrunA17_Chr5g0411151 84.874 238 32 3 1 235 1 237 3.06e-148 412
MsaG043783 MtrunA17_Chr5g0395181 69.767 215 56 3 19 233 7 212 4.48e-107 307
MsaG043783 MtrunA17_Chr4g0040071 72.059 204 53 4 31 233 13 213 6.46e-104 299
MsaG043783 MtrunA17_Chr1g0179781 55.825 206 72 6 33 229 7 202 1.16e-72 219
MsaG043783 MtrunA17_Chr7g0265291 58.333 204 71 5 33 229 7 203 2.90e-71 216
MsaG043783 MtrunA17_Chr2g0308401 49.265 136 59 4 34 159 5 140 7.05e-39 132
MsaG043783 MtrunA17_Chr4g0020811 47.794 136 61 3 34 159 7 142 9.48e-39 132
MsaG043783 MtrunA17_Chr4g0065041 48.031 127 65 1 33 159 8 133 7.09e-37 130
MsaG043783 MtrunA17_Chr7g0230511 34.568 243 113 7 30 228 6 246 9.09e-37 129
MsaG043783 MtrunA17_Chr2g0297251 45.669 127 68 1 33 159 37 162 1.30e-36 129
MsaG043783 MtrunA17_Chr2g0320521 34.052 232 109 6 34 228 7 231 4.26e-36 127
MsaG043783 MtrunA17_Chr2g0320561 46.715 137 63 4 34 160 5 141 7.73e-32 116
MsaG043783 MtrunA17_Chr2g0320551 43.382 136 67 3 34 159 5 140 4.94e-31 112
MsaG043783 MtrunA17_Chr4g0052451 43.902 82 40 1 85 160 11 92 4.34e-20 81.6
MsaG043783 MtrunA17_Chr8g0392151 33.951 162 82 6 19 159 1 158 1.14e-16 76.6
MsaG043783 MtrunA17_Chr8g0386511 29.193 161 99 4 72 228 4 153 2.72e-15 70.9
MsaG043783 MtrunA17_Chr5g0434331 27.670 206 105 9 29 228 39 206 1.08e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043783 AT4G17900.1 72.961 233 53 5 1 229 1 227 7.44e-121 343
MsaG043783 AT1G32700.1 70.968 217 53 5 24 235 2 213 2.35e-103 298
MsaG043783 AT4G17900.2 72.414 203 47 4 30 229 17 213 1.82e-102 296
MsaG043783 AT1G32700.2 70.391 179 44 4 61 235 1 174 6.78e-80 237
MsaG043783 AT5G46710.1 52.521 238 90 8 1 228 1 225 1.38e-77 233
MsaG043783 AT1G21000.2 49.583 240 82 4 26 228 6 243 1.68e-75 229
MsaG043783 AT1G21000.1 49.583 240 82 4 26 228 9 246 1.75e-75 229
MsaG043783 AT1G76590.1 50.439 228 81 4 33 228 18 245 1.00e-72 222
MsaG043783 AT5G46710.3 52.830 212 84 6 26 228 5 209 6.69e-72 218
MsaG043783 AT5G46710.2 59.627 161 57 3 1 159 1 155 2.23e-66 203
MsaG043783 AT1G43000.2 46.083 217 101 4 26 228 9 223 1.23e-61 192
MsaG043783 AT1G43000.1 46.083 217 101 4 26 228 2 216 1.90e-61 192
MsaG043783 AT2G27930.1 44.712 208 83 5 33 230 4 189 8.34e-53 169
MsaG043783 AT2G27930.4 44.712 208 83 5 33 230 4 189 8.34e-53 169
MsaG043783 AT2G27930.3 55.474 137 51 2 33 159 4 140 1.26e-48 157
MsaG043783 AT2G27930.2 55.474 137 51 2 33 159 4 140 1.26e-48 157
MsaG043783 AT2G12646.3 48.819 127 64 1 33 159 3 128 3.71e-37 131
MsaG043783 AT2G12646.1 48.819 127 64 1 33 159 6 131 4.09e-37 131
MsaG043783 AT2G12646.2 48.819 127 64 1 33 159 6 131 4.09e-37 131
MsaG043783 AT3G60670.1 46.212 132 70 1 28 159 2 132 1.19e-35 127
MsaG043783 AT2G27930.5 45.455 154 53 4 86 230 61 192 7.57e-34 120
MsaG043783 AT2G27930.6 45.455 154 53 4 86 230 61 192 7.57e-34 120
MsaG043783 AT1G31040.1 34.649 228 113 6 33 228 19 242 8.88e-34 122
MsaG043783 AT2G01818.1 34.579 214 115 9 35 228 13 221 5.00e-22 90.9
MsaG043783 AT2G01818.2 33.987 153 81 7 92 228 9 157 9.43e-11 58.9

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTCCCTTTCTTCTTT+TGG 0.157849 8:+51086091 None:intergenic
CAAAGATCATCGTGCTATTC+AGG 0.214126 8:-51087635 MsaT043783.1:intron
CCTTGCACTGTTGATAATAT+AGG 0.282719 8:+51087043 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8:-51087109 MsaT043783.1:CDS
AAGCTGACTTGTTCTTCTTC+TGG 0.332067 8:+51086240 None:intergenic
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8:-51086933 MsaT043783.1:CDS
AACTATGAATTCTAGATTGT+TGG 0.377091 8:-51086278 MsaT043783.1:intron
TTCATACCATGATGTGATTA+GGG 0.385029 8:-51087108 MsaT043783.1:CDS
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8:-51087074 MsaT043783.1:CDS
ACTGTTGGTGGTGGTGTTGA+AGG 0.411165 8:+51086128 None:intergenic
TGAGTGCAATATGTATTGTT+TGG 0.429253 8:-51087701 MsaT043783.1:CDS
TGGAAGACTGAAGAACATTA+AGG 0.432710 8:-51086165 MsaT043783.1:CDS
TATTGTTTGGATTGTATGAA+TGG 0.443604 8:-51087688 MsaT043783.1:CDS
TGTGTTTGATACAGATAAGA+AGG 0.447190 8:-51087133 MsaT043783.1:intron
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8:-51087801 MsaT043783.1:CDS
GCAGCACTAGTCCTCCCATC+GGG 0.455937 8:+51086060 None:intergenic
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8:-51086185 MsaT043783.1:CDS
TAATGTTCTTCAGTCTTCCA+TGG 0.466799 8:+51086168 None:intergenic
CAGATCAGCCAAAAGAAGAA+AGG 0.467780 8:-51086099 MsaT043783.1:CDS
GAATAGCACGATGATCTTTG+TGG 0.487973 8:+51087638 None:intergenic
GCTGATCTGTAATTGACTGT+TGG 0.490334 8:+51086113 None:intergenic
CCTCAGCCAAGGCCCGGAAA+AGG 0.495211 8:-51086999 MsaT043783.1:CDS
AGATCAGCCAAAAGAAGAAA+GGG 0.506864 8:-51086098 MsaT043783.1:CDS
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8:-51086925 MsaT043783.1:intron
CCTATATTATCAACAGTGCA+AGG 0.522886 8:-51087043 MsaT043783.1:CDS
GAAAGGCCTCAGCCAAGGCC+CGG 0.523082 8:-51087005 MsaT043783.1:CDS
GATCTGTAATTGACTGTTGG+TGG 0.533120 8:+51086116 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.534739 8:+51086119 None:intergenic
GGGTTGTGTTCTTGAATGAA+AGG 0.538393 8:-51087022 MsaT043783.1:CDS
TGCAGCACTAGTCCTCCCAT+CGG 0.541315 8:+51086059 None:intergenic
ATTCCGCATCGTGCCCCGAT+GGG 0.553697 8:-51086074 MsaT043783.1:CDS
AATTCCGCATCGTGCCCCGA+TGG 0.556752 8:-51086075 MsaT043783.1:CDS
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8:+51086971 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8:+51087102 None:intergenic
CCGCATCGTGCCCCGATGGG+AGG 0.573029 8:-51086071 MsaT043783.1:CDS
TTGAGCAGAGGTTTCAACCA+TGG 0.583253 8:+51087772 None:intergenic
AAGAAACGCTCTTTGAGCAG+AGG 0.583635 8:+51087760 None:intergenic
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8:-51087084 MsaT043783.1:CDS
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8:-51087789 MsaT043783.1:CDS
TGAATGAAAGGCCTCAGCCA+AGG 0.624070 8:-51087010 MsaT043783.1:CDS
CCTCCCATCGGGGCACGATG+CGG 0.625934 8:+51086071 None:intergenic
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8:+51086932 None:intergenic
AGCAGAGGTTTCAACCATGG+TGG 0.633067 8:+51087775 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8:+51086943 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8:-51087809 MsaT043783.1:CDS
TTCAATGATATCAGACTCAG+AGG 0.648076 8:-51086213 MsaT043783.1:CDS
CTATATTATCAACAGTGCAA+GGG 0.657853 8:-51087042 MsaT043783.1:CDS
CTGATGATGAGGAAAACAGG+TGG 0.717367 8:-51087798 MsaT043783.1:CDS
CAGCACTAGTCCTCCCATCG+GGG 0.730442 8:+51086061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 51086052 51088570 51086052 ID=MsaG043783
Chr8 mRNA 51086052 51088570 51086052 ID=MsaT043783.1;Parent=MsaG043783
Chr8 exon 51086052 51086285 51086052 ID=MsaT043783.1.exon4;Parent=MsaT043783.1
Chr8 CDS 51086052 51086285 51086052 ID=cds.MsaT043783.1;Parent=MsaT043783.1
Chr8 exon 51086926 51087141 51086926 ID=MsaT043783.1.exon3;Parent=MsaT043783.1
Chr8 CDS 51086926 51087141 51086926 ID=cds.MsaT043783.1;Parent=MsaT043783.1
Chr8 exon 51087636 51087833 51087636 ID=MsaT043783.1.exon2;Parent=MsaT043783.1
Chr8 CDS 51087636 51087833 51087636 ID=cds.MsaT043783.1;Parent=MsaT043783.1
Chr8 exon 51088511 51088570 51088511 ID=MsaT043783.1.exon1;Parent=MsaT043783.1
Chr8 CDS 51088511 51088570 51088511 ID=cds.MsaT043783.1;Parent=MsaT043783.1
Gene Sequence

>MsaT043783.1

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTGCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGCGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGATAAGAAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAAAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGATTGTTGGAACTTCAATAAATTTCCAGAAGAAGAACAAGTCAGCTTGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTGAAGAACATTAAGGTGCAAAGTTTCACTCCTTCAACACCACCACCAACAGTCAATTACAGATCAGCCAAAAGAAGAAAGGGAATTCCGCATCGTGCCCCGATGGGAGGACTAGTGCTGCAATACTAA
Protein sequence

>MsaT043783.1

MAFENQDTVREIKPKNRRIMGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSINFQKKNKSACASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVLQY*