AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043799 MtrunA17_Chr8g0366751 96.302 649 23 1 1 649 1 648 0.0 1291
MsaG043799 MtrunA17_Chr5g0411341 59.885 698 205 19 3 647 2 677 0.0 710
MsaG043799 MtrunA17_Chr5g0411331 61.905 210 73 4 34 238 5 212 1.45e-84 266
MsaG043799 MtrunA17_Chr8g0346161 47.143 210 82 4 445 644 273 463 1.68e-52 187
MsaG043799 MtrunA17_Chr8g0346161 30.244 205 106 5 43 243 19 190 2.82e-24 106
MsaG043799 MtrunA17_Chr8g0346151 49.223 193 90 3 445 636 261 446 1.83e-50 181
MsaG043799 MtrunA17_Chr8g0346151 23.256 301 157 7 67 333 36 296 2.93e-18 88.2
MsaG043799 MtrunA17_Chr7g0260841 39.035 228 86 5 414 623 288 480 3.76e-36 142
MsaG043799 MtrunA17_Chr7g0260841 29.348 184 122 6 53 234 49 226 8.49e-15 77.4
MsaG043799 MtrunA17_Chr7g0275821 39.474 190 89 4 455 640 244 411 1.77e-34 136
MsaG043799 MtrunA17_Chr1g0185621 32.105 190 113 5 54 234 11 193 6.77e-22 100
MsaG043799 MtrunA17_Chr1g0185621 32.099 162 95 2 463 618 475 627 2.57e-19 92.4
MsaG043799 MtrunA17_Chr4g0033391 35.329 167 84 4 444 610 138 280 3.32e-20 92.4
MsaG043799 MtrunA17_Chr5g0400671 36.970 165 71 5 452 609 147 285 1.08e-19 90.9
MsaG043799 MtrunA17_Chr7g0225901 36.943 157 86 3 463 618 88 232 2.06e-19 88.2
MsaG043799 MtrunA17_Chr0c01g0489461 29.592 294 163 14 385 641 108 394 2.54e-19 90.9
MsaG043799 MtrunA17_Chr2g0333641 28.182 220 123 4 397 610 81 271 4.48e-19 88.6
MsaG043799 MtrunA17_Chr4g0054581 31.401 207 119 5 449 645 190 383 5.47e-19 89.4
MsaG043799 MtrunA17_Chr4g0002801 29.870 231 135 6 382 610 89 294 1.58e-18 87.4
MsaG043799 MtrunA17_Chr4g0002781 28.270 237 135 5 382 610 104 313 2.00e-18 87.4
MsaG043799 MtrunA17_Chr2g0307131 35.976 164 87 5 449 610 168 315 2.81e-18 87.0
MsaG043799 MtrunA17_Chr2g0280161 32.571 175 107 3 435 609 150 313 3.72e-18 86.7
MsaG043799 MtrunA17_Chr4g0028071 33.333 162 93 6 467 625 153 302 5.63e-18 85.9
MsaG043799 MtrunA17_Chr2g0307141 35.333 150 82 4 463 610 183 319 8.38e-18 85.5
MsaG043799 MtrunA17_Chr4g0033291 28.922 204 105 4 410 610 122 288 4.67e-17 82.8
MsaG043799 MtrunA17_Chr4g0049331 33.333 150 87 3 461 609 133 270 5.88e-17 82.4
MsaG043799 MtrunA17_Chr6g0468491 36.184 152 78 4 463 610 137 273 1.24e-16 81.3
MsaG043799 MtrunA17_Chr2g0333621 27.442 215 134 5 397 610 82 275 1.43e-16 81.3
MsaG043799 MtrunA17_Chr5g0400641 32.215 149 90 2 467 615 2 139 1.65e-16 77.4
MsaG043799 MtrunA17_Chr8g0386921 28.261 230 144 8 69 283 19 242 1.75e-16 83.2
MsaG043799 MtrunA17_Chr8g0386921 28.834 163 101 3 459 606 402 564 8.39e-13 71.6
MsaG043799 MtrunA17_Chr3g0131691 35.256 156 96 3 461 614 296 448 2.93e-16 82.0
MsaG043799 MtrunA17_Chr5g0400621 31.515 165 100 3 446 610 142 293 4.75e-16 80.1
MsaG043799 MtrunA17_Chr4g0054571 31.122 196 94 4 439 605 105 288 4.97e-16 79.7
MsaG043799 MtrunA17_Chr4g0033281 29.557 203 104 4 410 610 120 285 6.25e-16 79.7
MsaG043799 MtrunA17_Chr2g0298751 33.540 161 83 4 464 611 53 202 1.93e-15 76.6
MsaG043799 MtrunA17_Chr8g0365711 33.544 158 92 5 468 619 249 399 3.66e-15 78.2
MsaG043799 MtrunA17_Chr2g0320291 30.247 162 94 5 467 625 137 282 4.03e-15 77.0
MsaG043799 MtrunA17_Chr8g0336681 30.058 173 101 4 447 610 138 299 5.35e-15 77.0
MsaG043799 MtrunA17_Chr1g0211081 31.325 166 89 6 467 611 321 482 1.64e-14 76.6
MsaG043799 MtrunA17_Chr7g0252041 33.333 162 101 3 461 618 284 442 8.48e-14 74.3
MsaG043799 MtrunA17_Chr5g0400661 45.833 72 39 0 445 516 131 202 1.37e-13 72.4
MsaG043799 MtrunA17_Chr4g0054561 31.677 161 91 5 451 610 147 289 5.13e-13 70.9
MsaG043799 MtrunA17_Chr4g0054621 31.469 143 80 3 463 605 172 296 5.74e-13 70.9
MsaG043799 MtrunA17_Chr6g0468461 39.062 128 59 4 389 516 118 226 7.05e-13 70.5
MsaG043799 MtrunA17_Chr4g0054551 30.337 178 94 6 440 610 130 284 1.22e-12 69.7
MsaG043799 MtrunA17_Chr5g0426851 26.147 218 137 8 436 635 70 281 3.82e-12 67.8
MsaG043799 MtrunA17_Chr2g0333611 28.667 150 88 3 464 610 46 179 1.06e-11 65.1
MsaG043799 MtrunA17_Chr4g0052821 28.662 157 103 3 457 609 284 435 2.76e-11 66.2
MsaG043799 MtrunA17_Chr8g0366551 28.070 171 120 2 462 629 124 294 5.20e-11 64.3
MsaG043799 MtrunA17_Chr1g0158941 23.894 226 139 6 430 626 53 274 5.26e-11 64.3
MsaG043799 MtrunA17_Chr4g0052841 29.240 171 114 3 464 630 293 460 5.50e-11 65.5
MsaG043799 MtrunA17_Chr2g0287761 26.486 185 114 6 462 633 1 176 6.24e-11 62.4
MsaG043799 MtrunA17_Chr3g0132471 27.451 204 110 6 446 623 96 287 9.63e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043799 AT1G32640.1 50.599 668 249 18 3 644 11 623 0.0 575
MsaG043799 AT4G17880.1 47.496 659 239 18 3 646 22 588 0.0 533
MsaG043799 AT5G46760.1 47.167 653 254 17 6 645 16 590 3.22e-169 496
MsaG043799 AT5G46830.1 57.068 191 67 4 454 643 334 510 1.64e-56 200
MsaG043799 AT5G46830.1 32.474 388 169 9 6 377 5 315 8.33e-43 162
MsaG043799 AT1G01260.1 38.202 267 112 6 374 640 362 575 3.72e-40 155
MsaG043799 AT1G01260.1 38.144 194 106 8 52 234 46 236 2.56e-25 111
MsaG043799 AT1G01260.2 38.202 267 112 6 374 640 362 575 3.72e-40 155
MsaG043799 AT1G01260.2 38.144 194 106 8 52 234 46 236 2.56e-25 111
MsaG043799 AT1G01260.3 38.202 267 112 6 374 640 362 575 3.72e-40 155
MsaG043799 AT1G01260.3 38.144 194 106 8 52 234 46 236 2.56e-25 111
MsaG043799 AT2G46510.1 42.051 195 82 3 445 637 377 542 2.52e-39 153
MsaG043799 AT2G46510.1 33.493 209 118 9 44 234 34 239 6.00e-21 97.4
MsaG043799 AT4G16430.1 40.341 176 69 4 445 619 302 442 3.58e-33 133
MsaG043799 AT4G16430.1 27.679 224 136 6 19 234 21 226 1.31e-16 83.6
MsaG043799 AT4G00870.1 46.000 150 73 2 462 611 248 389 7.97e-31 125
MsaG043799 AT4G00870.1 28.283 198 111 5 53 245 34 205 9.25e-17 83.6
MsaG043799 AT4G09820.1 34.343 198 114 7 58 245 20 211 6.61e-22 100
MsaG043799 AT4G09820.1 32.051 156 74 3 460 615 360 483 3.00e-16 82.4
MsaG043799 AT5G57150.4 31.844 179 116 3 464 640 56 230 1.28e-20 92.4
MsaG043799 AT5G57150.2 37.500 144 84 3 464 605 56 195 2.70e-20 90.5
MsaG043799 AT5G57150.5 37.500 144 84 3 464 605 55 194 2.91e-20 90.5
MsaG043799 AT5G57150.3 37.500 144 84 3 464 605 56 195 3.12e-20 90.1
MsaG043799 AT5G57150.1 37.500 144 84 3 464 605 55 194 6.10e-20 90.1
MsaG043799 AT4G37850.2 34.807 181 94 6 441 610 123 290 9.52e-20 91.3
MsaG043799 AT4G37850.1 34.807 181 94 6 441 610 123 290 9.52e-20 91.3
MsaG043799 AT1G63650.3 33.516 182 100 6 69 234 26 202 9.05e-18 87.8
MsaG043799 AT1G63650.3 24.551 167 119 1 459 618 401 567 1.40e-14 77.4
MsaG043799 AT1G63650.1 33.516 182 100 6 69 234 26 202 9.05e-18 87.8
MsaG043799 AT1G63650.1 24.551 167 119 1 459 618 401 567 1.40e-14 77.4
MsaG043799 AT1G63650.2 33.516 182 100 6 69 234 26 202 9.05e-18 87.8
MsaG043799 AT1G63650.2 24.551 167 119 1 459 618 401 567 1.40e-14 77.4
MsaG043799 AT3G26744.6 32.850 207 124 6 420 618 262 461 1.42e-17 86.7
MsaG043799 AT3G26744.1 32.850 207 124 6 420 618 262 461 1.69e-17 86.3
MsaG043799 AT3G26744.4 32.850 207 124 6 420 618 262 461 1.69e-17 86.3
MsaG043799 AT3G26744.2 32.850 207 124 6 420 618 262 461 1.69e-17 86.3
MsaG043799 AT2G28160.1 36.029 136 76 4 467 601 135 260 5.72e-17 82.8
MsaG043799 AT2G28160.2 36.029 136 76 4 467 601 135 260 6.12e-17 82.8
MsaG043799 AT3G24140.1 29.048 210 126 6 445 633 177 384 6.69e-17 84.0
MsaG043799 AT3G24140.2 29.048 210 126 6 445 633 177 384 7.95e-17 83.6
MsaG043799 AT4G29930.5 31.875 160 83 4 447 605 43 177 1.84e-16 79.7
MsaG043799 AT1G12860.2 33.929 168 86 4 461 618 265 417 3.29e-16 82.0
MsaG043799 AT4G29930.2 31.875 160 83 4 447 605 43 177 3.71e-16 78.6
MsaG043799 AT1G12860.1 33.929 168 86 4 461 618 265 417 5.19e-16 81.6
MsaG043799 AT4G29930.6 31.677 161 85 4 447 605 43 180 5.33e-16 78.2
MsaG043799 AT2G22750.4 30.047 213 130 4 407 610 93 295 9.99e-16 79.3
MsaG043799 AT2G22750.5 30.047 213 130 4 407 610 89 291 1.25e-15 79.0
MsaG043799 AT2G22750.3 30.047 213 130 4 407 610 93 295 1.26e-15 79.0
MsaG043799 AT2G22750.1 30.047 213 130 4 407 610 65 267 1.69e-15 78.2
MsaG043799 AT2G22750.2 30.047 213 130 4 407 610 65 267 1.88e-15 78.2
MsaG043799 AT4G29930.3 33.987 153 89 4 464 605 55 206 2.74e-15 77.0
MsaG043799 AT4G29930.7 33.987 153 89 4 464 605 55 206 2.74e-15 77.0
MsaG043799 AT4G29930.1 33.987 153 89 4 464 605 55 206 4.42e-15 76.3
MsaG043799 AT4G00480.3 29.348 184 104 6 69 231 36 214 3.59e-14 75.9
MsaG043799 AT5G41315.2 27.429 175 113 4 457 618 443 616 4.22e-14 76.3
MsaG043799 AT5G41315.2 27.957 186 108 5 69 234 35 214 6.52e-14 75.5
MsaG043799 AT5G41315.3 27.429 175 113 4 457 618 440 613 4.39e-14 76.3
MsaG043799 AT5G41315.3 27.957 186 108 5 69 234 32 211 6.55e-14 75.5
MsaG043799 AT5G41315.1 27.429 175 113 4 457 618 435 608 4.40e-14 76.3
MsaG043799 AT5G41315.1 27.957 186 108 5 69 234 27 206 6.62e-14 75.5
MsaG043799 AT4G00480.1 27.523 218 130 7 35 231 4 214 4.50e-14 75.9
MsaG043799 AT4G00480.2 29.348 184 104 6 69 231 36 214 5.93e-14 75.5
MsaG043799 AT4G29930.4 54.237 59 27 0 465 523 56 114 1.42e-13 70.1
MsaG043799 AT2G22760.2 32.738 168 83 5 452 610 112 258 6.81e-13 70.1
MsaG043799 AT2G16910.1 30.189 159 94 4 467 611 318 473 9.37e-13 71.6
MsaG043799 AT2G22760.1 32.738 168 83 5 452 610 112 258 1.08e-12 69.7
MsaG043799 AT1G10610.3 34.028 144 84 5 464 605 168 302 1.79e-12 69.7
MsaG043799 AT5G10570.1 29.231 195 111 5 436 630 126 293 1.89e-12 69.3
MsaG043799 AT1G10610.2 34.028 144 84 5 464 605 235 369 2.39e-12 69.7
MsaG043799 AT1G10610.1 34.028 144 84 5 464 605 265 399 2.63e-12 69.7
MsaG043799 AT2G22770.1 28.191 188 113 4 457 629 123 303 6.20e-12 67.8

Find 165 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGTAGAGCGTAGAATTTC+TGG 0.101182 8:-51376743 None:intergenic
CAGCGATGCTTTGTCCATTT+TGG 0.142541 8:-51376784 None:intergenic
TCCTTGTGCATCACCAATTT+TGG 0.145524 8:-51377237 None:intergenic
TCTCTTCTTTCTTCCAAAAT+TGG 0.173669 8:+51377224 MsaT043799.1:CDS
CTTCGATGAGCTTGTTCTTC+TGG 0.206409 8:-51375643 None:intergenic
GAGCTTAATACGTTGATTTC+AGG 0.216229 8:+51375679 MsaT043799.1:CDS
GGATATTGAAGTGAAGATTA+TGG 0.243770 8:+51377016 MsaT043799.1:CDS
CTCAGAATCTCCCCGGATTC+TGG 0.249063 8:-51376348 None:intergenic
TGGCTACACCGCTGCTCGGC+TGG 0.254416 8:+51375563 MsaT043799.1:CDS
CCTCCGACCTCTCTAATTTC+TGG 0.263738 8:+51375368 MsaT043799.1:CDS
GGTTCCAAATGTTTCCAAAA+TGG 0.283532 8:+51376770 MsaT043799.1:CDS
TCCATCGTCAATGCTGCTTC+TGG 0.290947 8:-51376481 None:intergenic
AGTTCTTATGTCACCGGAAA+TGG 0.307455 8:+51376390 MsaT043799.1:CDS
AACGGCCACGAACCTGTTTC+AGG 0.325408 8:-51376030 None:intergenic
CCAACAATATGAATAACATT+TGG 0.329630 8:+51375308 None:intergenic
GGCTACACCGCTGCTCGGCT+GGG 0.333611 8:+51375564 MsaT043799.1:CDS
TCCATACAACGCTGATCTAA+TGG 0.334173 8:+51375975 MsaT043799.1:CDS
TGCAGAAATGGTTGCGTTCA+TGG 0.335709 8:-51376166 None:intergenic
CCGCTGCTCGGCTGGGGTGA+TGG 0.351635 8:+51375571 MsaT043799.1:CDS
CTCTTCTAAGTTAATTGACT+TGG 0.357805 8:+51376995 MsaT043799.1:CDS
GGTCAGTCACAGTTCGTTGC+CGG 0.380141 8:+51376426 MsaT043799.1:CDS
GCCCGTGAAGCACGGGTCCA+TGG 0.380533 8:+51375883 MsaT043799.1:CDS
CGTTTCTTCGGTCGCTTCCC+TGG 0.384757 8:-51376642 None:intergenic
AGTAGCAGGGTGGTTGAGCC+AGG 0.388458 8:+51376624 MsaT043799.1:CDS
AGGTGAATTGGAGAAACAAT+TGG 0.390081 8:+51376872 MsaT043799.1:CDS
AATTTCAACAATCCTGAAAC+AGG 0.392528 8:+51376018 MsaT043799.1:CDS
TCAACCCCGGTGTGGATCAC+CGG 0.394753 8:+51375826 MsaT043799.1:CDS
GGAATGCTTTCGTTTACTTC+CGG 0.395195 8:+51376501 MsaT043799.1:CDS
CCCATGGACCCGTGCTTCAC+GGG 0.396142 8:-51375884 None:intergenic
AGAAAAGAAAGTTCCATTTC+CGG 0.399215 8:-51376403 None:intergenic
TGAAGGAAGGAGAGAGTAGC+AGG 0.399337 8:+51376610 MsaT043799.1:CDS
CGTCTTCAAGCTCTGATTGA+AGG 0.400185 8:+51375484 MsaT043799.1:CDS
TCCATTAGATCAGCGTTGTA+TGG 0.401213 8:-51375976 None:intergenic
ATCGTTCTCGCTCGTTGTAA+TGG 0.403129 8:-51376058 None:intergenic
TGGGTTGGGATGCTATGATT+AGG 0.406285 8:+51377036 MsaT043799.1:CDS
GATATTGAAGTGAAGATTAT+GGG 0.406522 8:+51377017 MsaT043799.1:CDS
CAACAATATGAATAACATTT+GGG 0.406989 8:+51375309 None:intergenic
TGGTGTTTCCGTTAGAGTAC+TGG 0.408218 8:-51376235 None:intergenic
TACTAATTCTGTCTGTGAAC+GGG 0.410519 8:+51375861 MsaT043799.1:CDS
AGGTACGGTGGCAGTGTTAA+CGG 0.412615 8:-51376142 None:intergenic
TCCGGGGAGATTCTGAGTTT+CGG 0.416724 8:+51376354 MsaT043799.1:CDS
TCATTACCGTTTGAAACAAA+TGG 0.429184 8:+51376207 MsaT043799.1:CDS
GGAATCGTCTAAAGGTGAAT+TGG 0.437083 8:+51376860 MsaT043799.1:CDS
CAGCCCCGGTGATCCACACC+GGG 0.439785 8:-51375831 None:intergenic
GTTGAACCATTTGTTTCAAA+CGG 0.440221 8:-51376213 None:intergenic
ACCCATGGACCCGTGCTTCA+CGG 0.442980 8:-51375885 None:intergenic
CAACCCCGGTGTGGATCACC+GGG 0.445985 8:+51375827 MsaT043799.1:CDS
GATTGTTGCATTTGCAGAAA+TGG 0.449234 8:-51376178 None:intergenic
CGAGGTAGAAAACCGGCTAA+TGG 0.451911 8:+51376663 MsaT043799.1:CDS
TCAATGCTGCTTCTGGATAC+AGG 0.460912 8:-51376474 None:intergenic
GGTTCAATGAAACCAGAATC+CGG 0.461046 8:+51376336 MsaT043799.1:CDS
AGAACGATCCTTCATCTGTC+TGG 0.464418 8:+51376073 MsaT043799.1:CDS
TCATCCTGCGGCGAAGTTGA+TGG 0.465184 8:+51377079 MsaT043799.1:CDS
CCCGTGAAGCACGGGTCCAT+GGG 0.472068 8:+51375884 MsaT043799.1:CDS
TGAAGTGAAGATTATGGGTT+GGG 0.485063 8:+51377022 MsaT043799.1:CDS
TGTGGATCACCGGGGCTGAA+AGG 0.486911 8:+51375836 MsaT043799.1:CDS
ATAAGCCTGGCTCAGTGATC+CGG 0.487579 8:-51375797 None:intergenic
TCAGCCCCGGTGATCCACAC+CGG 0.489282 8:-51375832 None:intergenic
GGTTCTTCTCTTCCATTAGC+CGG 0.494454 8:-51376675 None:intergenic
TCTCTCCGCCTCTGCAGCTA+TGG 0.499397 8:+51376104 MsaT043799.1:CDS
GTGAACGGGCCCGTGAAGCA+CGG 0.499419 8:+51375875 MsaT043799.1:CDS
GCTACACCGCTGCTCGGCTG+GGG 0.501207 8:+51375565 MsaT043799.1:CDS
TCAAGCAGCCGAGGTGGAGG+CGG 0.502710 8:+51376552 MsaT043799.1:CDS
GCCGCCATCAACTTCGCCGC+AGG 0.505374 8:-51377083 None:intergenic
ATTAGTAAGCCTTTCAGCCC+CGG 0.505431 8:-51375845 None:intergenic
CGATGAGCTTGTTCTTCTGG+AGG 0.507325 8:-51375640 None:intergenic
GGTCCATGGGTTTCAGACCT+TGG 0.507823 8:+51375897 MsaT043799.1:CDS
GGTGAATTGGAGAAACAATT+GGG 0.509336 8:+51376873 MsaT043799.1:CDS
GAAATCGAAGCTACAAGGGC+TGG 0.510964 8:+51376839 MsaT043799.1:CDS
TCAACAAGCTTCTGTTAACA+TGG 0.511118 8:+51377169 MsaT043799.1:CDS
TGTTGTGATGGAGGAGGCAC+TGG 0.512388 8:-51375430 None:intergenic
GCCGAGCAGCGGTGTAGCCA+TGG 0.518239 8:-51375560 None:intergenic
CAATCCTTTGTCAACGGCAC+CGG 0.524318 8:+51375778 MsaT043799.1:CDS
TTGAAGTGAAGATTATGGGT+TGG 0.525361 8:+51377021 MsaT043799.1:CDS
GAAATGGTTGCGTTCATGGC+AGG 0.525493 8:-51376162 None:intergenic
CGGCACCGGATCACTGAGCC+AGG 0.526951 8:+51375792 MsaT043799.1:CDS
TGAACGGGCCCGTGAAGCAC+GGG 0.527585 8:+51375876 MsaT043799.1:CDS
TCAGAGCTTGAAGACGGTGT+TGG 0.533151 8:-51375477 None:intergenic
AAATCAAGCAGCCGAGGTGG+AGG 0.534413 8:+51376549 MsaT043799.1:CDS
AAGAAAAGTTCTTATGTCAC+CGG 0.535645 8:+51376384 MsaT043799.1:CDS
TTGAAGGCGCAAAAGAAAGC+TGG 0.535975 8:+51375500 MsaT043799.1:CDS
TCCAAAATTGGTGATGCACA+AGG 0.537190 8:+51377236 MsaT043799.1:CDS
TCATTGAGCCAGACAGATGA+AGG 0.541127 8:-51376081 None:intergenic
GTTCAATGAAACCAGAATCC+GGG 0.541459 8:+51376337 MsaT043799.1:CDS
CTTGATTTCATATTTGAAGA+CGG 0.542319 8:-51376534 None:intergenic
AATTCCATTAGATAAAGAAA+AGG 0.545738 8:+51376950 MsaT043799.1:CDS
CAAGGGCTGGAATCGTCTAA+AGG 0.545942 8:+51376852 MsaT043799.1:CDS
TCTAGATGTTTCTGCTGTGA+AGG 0.546467 8:+51376593 MsaT043799.1:CDS
ATTTCTGCAAATGCAACAAT+CGG 0.548088 8:+51376180 MsaT043799.1:CDS
GGGGTGATGGATATTACAAA+GGG 0.549464 8:+51375584 MsaT043799.1:CDS
CTTAATACGTTGATTTCAGG+TGG 0.552157 8:+51375682 MsaT043799.1:CDS
GAATTGAAATCGAAGCTACA+AGG 0.554225 8:+51376834 MsaT043799.1:CDS
ACAATTGGGTGCAACAAAGA+AGG 0.555028 8:+51376887 MsaT043799.1:CDS
ACCGAAACTCAGAATCTCCC+CGG 0.557140 8:-51376355 None:intergenic
ACCATGGCTACACCGCTGCT+CGG 0.557176 8:+51375559 MsaT043799.1:CDS
AGCCATGGTGTAATCATAAG+AGG 0.557540 8:-51375545 None:intergenic
GGTTGCGTTCATGGCAGGTA+CGG 0.559424 8:-51376157 None:intergenic
TTACTAATTCTGTCTGTGAA+CGG 0.561586 8:+51375860 MsaT043799.1:CDS
GATGTTTCTGCTGTGAAGGA+AGG 0.568459 8:+51376597 MsaT043799.1:CDS
GAAATTAGAGAGGTCGGAGG+TGG 0.568481 8:-51375365 None:intergenic
TGAGCAAAGTTAACTGCAGT+TGG 0.569736 8:-51376255 None:intergenic
ATGGACAAAGCATCGCTGCT+AGG 0.569855 8:+51376789 MsaT043799.1:CDS
GAAGTTGCGGAATGTGGTGT+TGG 0.570968 8:-51375394 None:intergenic
TGCGTTCATGGCAGGTACGG+TGG 0.575421 8:-51376154 None:intergenic
GAAGAAACGAGGTAGAAAAC+CGG 0.575492 8:+51376656 MsaT043799.1:CDS
GTTGTTGAAGTTGCGGAATG+TGG 0.580780 8:-51375400 None:intergenic
GGACGACAACTCCTCCGTCA+TGG 0.585254 8:+51375330 MsaT043799.1:CDS
ACAATCCTGAAACAGGTTCG+TGG 0.586854 8:+51376025 MsaT043799.1:CDS
GGGTTGAATTGAAATAAGCC+TGG 0.588534 8:-51375810 None:intergenic
AAGCCAGAAATTAGAGAGGT+CGG 0.590490 8:-51375371 None:intergenic
CCATCACCCCAGCCGAGCAG+CGG 0.593117 8:-51375571 None:intergenic
GGCTTATTTCAATTCAACCC+CGG 0.593927 8:+51375813 MsaT043799.1:CDS
TCGAATTTCCATAGCTGCAG+AGG 0.593973 8:-51376112 None:intergenic
GTATACCAACGTCTTCAAGC+GGG 0.595425 8:+51375923 MsaT043799.1:CDS
TGATCCGGTGCCGTTGACAA+AGG 0.596939 8:-51375782 None:intergenic
ATTCTACGCTCTACGCGCTG+TGG 0.607114 8:+51376749 MsaT043799.1:CDS
AATTTCCATAGCTGCAGAGG+CGG 0.609907 8:-51376109 None:intergenic
ATGAAATCAAGCAGCCGAGG+TGG 0.610118 8:+51376546 MsaT043799.1:CDS
GTCAGTGACATCTTCCTCAA+CGG 0.610482 8:-51375725 None:intergenic
GGCGTTGAAAGACTTAGACT+TGG 0.610935 8:+51377103 MsaT043799.1:CDS
CAACAAGCTTCTGTTAACAT+GGG 0.613453 8:+51377170 MsaT043799.1:CDS
GTAGCAGGGTGGTTGAGCCA+GGG 0.614057 8:+51376625 MsaT043799.1:CDS
TGGTGCAGTTGTTGAAGTTG+CGG 0.614269 8:-51375407 None:intergenic
TGGTTCAACCAGTACTCTAA+CGG 0.614412 8:+51376227 MsaT043799.1:CDS
AAATCAACGTATTAAGCTCA+CGG 0.614664 8:-51375675 None:intergenic
CTTCAATCAGAGCTTGAAGA+CGG 0.614935 8:-51375483 None:intergenic
TCTGCTTCTACATGATTCAA+CGG 0.616515 8:-51376696 None:intergenic
TGTGATGGAGGAGGCACTGG+TGG 0.617060 8:-51375427 None:intergenic
TGTATCAGATGATGCCGTTG+AGG 0.617574 8:+51375711 MsaT043799.1:CDS
CAGAATCTCCGCCTCCACCT+CGG 0.617972 8:-51376560 None:intergenic
ATTTCAATTCAACCCCGGTG+TGG 0.618802 8:+51375818 MsaT043799.1:CDS
AACATAGACTGTTGTGATGG+AGG 0.624047 8:-51375439 None:intergenic
TTGAACATAGACTGTTGTGA+TGG 0.627469 8:-51375442 None:intergenic
ATGACTCAATCCTTTGTCAA+CGG 0.630282 8:+51375772 MsaT043799.1:CDS
CTCGTTGTAATGGAATTCAA+CGG 0.633434 8:-51376048 None:intergenic
GGAAGGAGAGAGTAGCAGGG+TGG 0.637372 8:+51376614 MsaT043799.1:CDS
TCCTGCGGCGAAGTTGATGG+CGG 0.639474 8:+51377082 MsaT043799.1:CDS
TCCAGAAGCAGCATTGACGA+TGG 0.640563 8:+51376480 MsaT043799.1:CDS
TGGGGTGATGGATATTACAA+AGG 0.644784 8:+51375583 MsaT043799.1:CDS
TGTATACCAACGTCTTCAAG+CGG 0.647168 8:+51375922 MsaT043799.1:CDS
ATACCAAGGTCTGAAACCCA+TGG 0.647395 8:-51375900 None:intergenic
TTCAATGAAACCAGAATCCG+GGG 0.651102 8:+51376338 MsaT043799.1:CDS
ACCACCCCGCTTGAAGACGT+TGG 0.653527 8:-51375928 None:intergenic
GGGAAGCGACCGAAGAAACG+AGG 0.656232 8:+51376645 MsaT043799.1:CDS
ATAGACTGTTGTGATGGAGG+AGG 0.660918 8:-51375436 None:intergenic
GGTCATGAAAGCTTCCATGA+CGG 0.664445 8:-51375344 None:intergenic
TATACCAACGTCTTCAAGCG+GGG 0.672915 8:+51375924 MsaT043799.1:CDS
TACAAAGGGGAAGATGACAA+AGG 0.675098 8:+51375598 MsaT043799.1:CDS
CATGAAAGCTTCCATGACGG+AGG 0.681601 8:-51375341 None:intergenic
CCAGAAATTAGAGAGGTCGG+AGG 0.681653 8:-51375368 None:intergenic
GAAGGAAGGAGAGAGTAGCA+GGG 0.683186 8:+51376611 MsaT043799.1:CDS
AATTGAAATCGAAGCTACAA+GGG 0.684780 8:+51376835 MsaT043799.1:CDS
AGACGTTGGTATACATACCA+AGG 0.685367 8:-51375914 None:intergenic
AATATGAAATCAAGCAGCCG+AGG 0.686490 8:+51376543 MsaT043799.1:CDS
GGATTGCCAGAAAATCGCGT+AGG 0.687237 8:-51375524 None:intergenic
TGAAGACGGTAAGACTACAC+CGG 0.689623 8:-51376520 None:intergenic
AAGATGTCACTGACACTGAG+TGG 0.696275 8:+51375734 MsaT043799.1:CDS
ATCCTCTTATGATTACACCA+TGG 0.705200 8:+51375543 MsaT043799.1:CDS
ACCAACGTCTTCAAGCGGGG+TGG 0.708219 8:+51375927 MsaT043799.1:CDS
TTGGAAGCCAGAAATTAGAG+AGG 0.708961 8:-51375375 None:intergenic
CAGCAAGAAGAATCATCCTG+CGG 0.713584 8:+51377067 MsaT043799.1:CDS
AGCCCCGGTGATCCACACCG+GGG 0.732020 8:-51375830 None:intergenic
TACGGTGGCAGTGTTAACGG+TGG 0.759784 8:-51376139 None:intergenic
AACCCCGGTGTGGATCACCG+GGG 0.772910 8:+51375828 MsaT043799.1:CDS
GGGTGATGGATATTACAAAG+GGG 0.787460 8:+51375585 MsaT043799.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 51375316 51377265 51375316 ID=MsaG043799
Chr8 mRNA 51375316 51377265 51375316 ID=MsaT043799.1;Parent=MsaG043799
Chr8 exon 51375316 51377265 51375316 ID=MsaT043799.1.exon1;Parent=MsaT043799.1
Chr8 CDS 51375316 51377265 51375316 ID=cds.MsaT043799.1;Parent=MsaT043799.1
Gene Sequence

>MsaT043799.1

ATGAATAACATTTGGGACGACAACTCCTCCGTCATGGAAGCTTTCATGACCACCTCCGACCTCTCTAATTTCTGGCTTCCAACACCACATTCCGCAACTTCAACAACTGCACCACCAGTGCCTCCTCCATCACAACAGTCTATGTTCAATCAAGAGACTCTCCAACACCGTCTTCAAGCTCTGATTGAAGGCGCAAAAGAAAGCTGGACCTACGCGATTTTCTGGCAATCCTCTTATGATTACACCATGGCTACACCGCTGCTCGGCTGGGGTGATGGATATTACAAAGGGGAAGATGACAAAGGAAAACTGAAACGAGTCACGCCTCCAGAAGAACAAGCTCATCGAAGAAAGATTCTCCGTGAGCTTAATACGTTGATTTCAGGTGGTTCGTCTGTATCAGATGATGCCGTTGAGGAAGATGTCACTGACACTGAGTGGTTTTTTCTCACGTCGATGACTCAATCCTTTGTCAACGGCACCGGATCACTGAGCCAGGCTTATTTCAATTCAACCCCGGTGTGGATCACCGGGGCTGAAAGGCTTACTAATTCTGTCTGTGAACGGGCCCGTGAAGCACGGGTCCATGGGTTTCAGACCTTGGTATGTATACCAACGTCTTCAAGCGGGGTGGTTGAGCTTGCTTCTACAGAAATGATTCCATACAACGCTGATCTAATGGAAAAGATTCGTGTTTTGTTTAATTTCAACAATCCTGAAACAGGTTCGTGGCCGTTGAATTCCATTACAACGAGCGAGAACGATCCTTCATCTGTCTGGCTCAATGATCTCTCCGCCTCTGCAGCTATGGAAATTCGAGATACCACCGTTAACACTGCCACCGTACCTGCCATGAACGCAACCATTTCTGCAAATGCAACAATCGGTAAATCATTACCGTTTGAAACAAATGGTTCAACCAGTACTCTAACGGAAACACCAACTGCAGTTAACTTTGCTCAGCGTCAGAATCAGAACAACCAGAACCAGAGCTTTTTTCTGAAGGAACTGAATTTTTCTGGTTCAATGAAACCAGAATCCGGGGAGATTCTGAGTTTCGGTGAAAGTAAGAAAAGTTCTTATGTCACCGGAAATGGAACTTTCTTTTCTGGTCAGTCACAGTTCGTTGCCGGTGAAGAAAACAGAAAAAGAAAGTCTCCTGTATCCAGAAGCAGCATTGACGATGGAATGCTTTCGTTTACTTCCGGTGTAGTCTTACCGTCTTCAAATATGAAATCAAGCAGCCGAGGTGGAGGCGGAGATTCTGATCATTCAGATCTAGATGTTTCTGCTGTGAAGGAAGGAGAGAGTAGCAGGGTGGTTGAGCCAGGGAAGCGACCGAAGAAACGAGGTAGAAAACCGGCTAATGGAAGAGAAGAACCGTTGAATCATGTAGAAGCAGAGAGACAGAGAAGAGAAAAGCTTAACCAGAAATTCTACGCTCTACGCGCTGTGGTTCCAAATGTTTCCAAAATGGACAAAGCATCGCTGCTAGGCGATGCTATTTCTTATATAAACGAATTGAAATCGAAGCTACAAGGGCTGGAATCGTCTAAAGGTGAATTGGAGAAACAATTGGGTGCAACAAAGAAGGAACTAGAACATGTTGCAAGCAAAAATCAAAATCAAAATCCAATTCCATTAGATAAAGAAAAGGAGAAAACAACAAGCTCAACAAGCTCTTCTAAGTTAATTGACTTGGATATTGAAGTGAAGATTATGGGTTGGGATGCTATGATTAGGATTCAATGCAGCAAGAAGAATCATCCTGCGGCGAAGTTGATGGCGGCGTTGAAAGACTTAGACTTGGATGTTAATCATGCTAGTGTGTCTGTTGTGAATGATTTGATGATTCAACAAGCTTCTGTTAACATGGGAAGTCGATTTTACACACAAGAACAACTTTTGTCTCTTCTTTCTTCCAAAATTGGTGATGCACAAGGAGATTGA
Protein sequence

>MsaT043799.1

MNNIWDDNSSVMEAFMTTSDLSNFWLPTPHSATSTTAPPVPPPSQQSMFNQETLQHRLQALIEGAKESWTYAIFWQSSYDYTMATPLLGWGDGYYKGEDDKGKLKRVTPPEEQAHRRKILRELNTLISGGSSVSDDAVEEDVTDTEWFFLTSMTQSFVNGTGSLSQAYFNSTPVWITGAERLTNSVCERAREARVHGFQTLVCIPTSSSGVVELASTEMIPYNADLMEKIRVLFNFNNPETGSWPLNSITTSENDPSSVWLNDLSASAAMEIRDTTVNTATVPAMNATISANATIGKSLPFETNGSTSTLTETPTAVNFAQRQNQNNQNQSFFLKELNFSGSMKPESGEILSFGESKKSSYVTGNGTFFSGQSQFVAGEENRKRKSPVSRSSIDDGMLSFTSGVVLPSSNMKSSSRGGGGDSDHSDLDVSAVKEGESSRVVEPGKRPKKRGRKPANGREEPLNHVEAERQRREKLNQKFYALRAVVPNVSKMDKASLLGDAISYINELKSKLQGLESSKGELEKQLGATKKELEHVASKNQNQNPIPLDKEKEKTTSSTSSSKLIDLDIEVKIMGWDAMIRIQCSKKNHPAAKLMAALKDLDLDVNHASVSVVNDLMIQQASVNMGSRFYTQEQLLSLLSSKIGDAQGD*