Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043813 | AET03325.1 | 64.249 | 193 | 18 | 1 | 1 | 142 | 243 | 435 | 3.67e-72 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043813 | sp|O04609|WRK22_ARATH | 34.694 | 147 | 66 | 8 | 16 | 155 | 63 | 186 | 2.13e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043813 | tr|G7LIF9|G7LIF9_MEDTR | 64.249 | 193 | 18 | 1 | 1 | 142 | 243 | 435 | 1.75e-72 | 236 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG043813 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043813 | MtrunA17_Chr8g0366921 | 88.462 | 78 | 9 | 0 | 1 | 78 | 243 | 320 | 3.15e-42 | 143 |
| MsaG043813 | MtrunA17_Chr6g0470151 | 42.373 | 118 | 54 | 2 | 1 | 118 | 248 | 351 | 6.77e-19 | 82.0 |
| MsaG043813 | MtrunA17_Chr2g0289181 | 34.884 | 129 | 34 | 1 | 84 | 162 | 1 | 129 | 4.34e-17 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG043813 | AT4G01250.1 | 34.694 | 147 | 66 | 8 | 16 | 155 | 63 | 186 | 2.17e-12 | 63.9 |
Find 38 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAATTTGATGACATATTC+TGG | 0.180052 | 8:-51578596 | None:intergenic |
| CGAAAACAGACACTCGATTT+TGG | 0.221712 | 8:-51577023 | None:intergenic |
| TGGGGTTCTTGTGGTTCTAA+TGG | 0.273735 | 8:-51577102 | None:intergenic |
| TGGATGTGGAGACCCTTTAA+TGG | 0.287579 | 8:-51577443 | None:intergenic |
| TAAAGTTGTAGAACTGGTTT+GGG | 0.308186 | 8:-51577157 | None:intergenic |
| CAGAATATGTCATCAAATTC+AGG | 0.324509 | 8:+51578597 | MsaT043813.1:CDS |
| GTTGAGTTCAGGATGAAAAT+TGG | 0.333089 | 8:-51577076 | None:intergenic |
| TCATGGAGATGGAGAAAATA+TGG | 0.338703 | 8:+51577412 | MsaT043813.1:CDS |
| ATAAGATTTCAGAAGATTCA+TGG | 0.343013 | 8:+51577395 | MsaT043813.1:CDS |
| GTAAAGTTGTAGAACTGGTT+TGG | 0.369929 | 8:-51577158 | None:intergenic |
| GTAGTCGATTATGATTTCTC+TGG | 0.378001 | 8:-51577122 | None:intergenic |
| GGATGTGGAGACCCTTTAAT+GGG | 0.385486 | 8:-51577442 | None:intergenic |
| ATTCAGTAAAGTTGTAGAAC+TGG | 0.405398 | 8:-51577163 | None:intergenic |
| ACTCGAGCCTTGCTGCTCTC+TGG | 0.411380 | 8:-51578705 | None:intergenic |
| TTGAGCAACATCCAGCTTCT+CGG | 0.416229 | 8:-51578632 | None:intergenic |
| TCTAATGGATGGTTGAGTTC+AGG | 0.423106 | 8:-51577087 | None:intergenic |
| TAGTCGATTATGATTTCTCT+GGG | 0.436337 | 8:-51577121 | None:intergenic |
| TGCTGCAACATTACCACATT+AGG | 0.438405 | 8:-51578675 | None:intergenic |
| GTTCTTGTGGTTCTAATGGA+TGG | 0.440210 | 8:-51577098 | None:intergenic |
| TCATCAAATTCAGGATTAGA+TGG | 0.442631 | 8:+51578606 | MsaT043813.1:CDS |
| TGAATTTGATGACATATTCT+GGG | 0.443651 | 8:-51578595 | None:intergenic |
| TGTTGTTGTTCAACGAGCAT+TGG | 0.450106 | 8:-51576988 | None:intergenic |
| TTAGATGGTGTCCGAGAAGC+TGG | 0.454965 | 8:+51578621 | MsaT043813.1:CDS |
| AATGCTCGTTGAACAACAAC+AGG | 0.486815 | 8:+51576990 | None:intergenic |
| CTGGTTTGGGGTAGTAGAAT+CGG | 0.494141 | 8:-51577144 | None:intergenic |
| TGATTTCTCTGGGGTTCTTG+TGG | 0.495150 | 8:-51577111 | None:intergenic |
| TTTCAGAAGATTCATGGAGA+TGG | 0.509856 | 8:+51577401 | MsaT043813.1:CDS |
| AAACTTTGATTCTCCTAATG+TGG | 0.514826 | 8:+51578662 | MsaT043813.1:CDS |
| GGTGGTTGTAGTCTTAATGC+AGG | 0.525304 | 8:-51577055 | None:intergenic |
| AAATAATAACCAAGTGACAT+TGG | 0.536349 | 8:+51577354 | MsaT043813.1:CDS |
| CATAGCTCACCAATGTCACT+TGG | 0.536490 | 8:-51577363 | None:intergenic |
| TTAAAGGGTCTCCACATCCA+AGG | 0.584498 | 8:+51577446 | MsaT043813.1:CDS |
| GAGTTCAGGATGAAAATTGG+TGG | 0.596987 | 8:-51577073 | None:intergenic |
| AGCAAGGCTCGAGTTCATGA+TGG | 0.606224 | 8:+51578714 | MsaT043813.1:CDS |
| TGATCATCCAGAGAGCAGCA+AGG | 0.696293 | 8:+51578698 | MsaT043813.1:CDS |
| GACATTGGTGAGCTATGTGA+AGG | 0.706817 | 8:+51577369 | MsaT043813.1:CDS |
| AGTCGATTATGATTTCTCTG+GGG | 0.721816 | 8:-51577120 | None:intergenic |
| AAAGTTGTAGAACTGGTTTG+GGG | 0.727319 | 8:-51577156 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 51576991 | 51578743 | 51576991 | ID=MsaG043813 |
| Chr8 | mRNA | 51576991 | 51578743 | 51576991 | ID=MsaT043813.1;Parent=MsaG043813 |
| Chr8 | exon | 51576991 | 51577223 | 51576991 | ID=MsaT043813.1.exon1;Parent=MsaT043813.1 |
| Chr8 | CDS | 51576991 | 51577223 | 51576991 | ID=cds.MsaT043813.1;Parent=MsaT043813.1 |
| Chr8 | exon | 51577351 | 51577467 | 51577351 | ID=MsaT043813.1.exon2;Parent=MsaT043813.1 |
| Chr8 | CDS | 51577351 | 51577467 | 51577351 | ID=cds.MsaT043813.1;Parent=MsaT043813.1 |
| Chr8 | exon | 51578593 | 51578743 | 51578593 | ID=MsaT043813.1.exon3;Parent=MsaT043813.1 |
| Chr8 | CDS | 51578593 | 51578743 | 51578593 | ID=cds.MsaT043813.1;Parent=MsaT043813.1 |
| Gene Sequence |
| Protein sequence |
