AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043876

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043876 MtrunA17_Chr8g0366921 64.138 290 89 2 1 279 35 320 3.77e-74 235
MsaG043876 MtrunA17_Chr6g0470151 44.548 321 155 6 7 321 50 353 5.09e-53 182
MsaG043876 MtrunA17_Chr2g0289181 56.643 143 62 0 285 427 1 143 1.12e-46 160
MsaG043876 MtrunA17_Chr8g0368721 48.421 285 120 5 7 279 43 312 1.66e-35 133
MsaG043876 MtrunA17_Chr1g0202461 55.000 100 43 2 275 374 239 336 2.22e-28 115
MsaG043876 MtrunA17_Chr8g0348311 48.413 126 53 3 268 391 134 249 6.92e-26 107
MsaG043876 MtrunA17_Chr7g0248691 46.875 128 51 3 243 370 115 225 7.48e-24 101
MsaG043876 MtrunA17_Chr2g0293901 50.769 130 50 2 15 144 54 169 1.90e-23 97.8
MsaG043876 MtrunA17_Chr8g0387501 62.500 72 26 1 299 370 130 200 1.99e-22 96.7
MsaG043876 MtrunA17_Chr7g0266621 40.830 289 134 4 3 279 39 302 2.38e-22 97.1
MsaG043876 MtrunA17_Chr4g0053281 58.333 72 29 1 299 369 267 338 1.95e-19 89.0
MsaG043876 MtrunA17_Chr2g0293481 52.564 78 36 1 293 370 262 338 1.21e-18 88.2
MsaG043876 MtrunA17_Chr2g0284321 38.816 152 69 6 299 431 36 182 1.31e-18 84.3
MsaG043876 MtrunA17_Chr7g0249311 50.000 86 41 2 288 373 169 252 1.90e-18 88.2
MsaG043876 MtrunA17_Chr7g0249311 47.297 74 38 1 296 369 390 462 2.51e-14 75.1
MsaG043876 MtrunA17_Chr2g0319961 63.333 60 22 0 299 358 81 140 2.04e-18 84.7
MsaG043876 MtrunA17_Chr1g0184691 62.121 66 21 1 299 360 294 359 2.53e-18 86.3
MsaG043876 MtrunA17_Chr5g0401821 55.556 72 31 1 284 355 186 256 3.22e-18 84.3
MsaG043876 MtrunA17_Chr3g0139021 51.190 84 36 2 278 357 291 373 3.29e-18 86.3
MsaG043876 MtrunA17_Chr3g0079691 32.917 240 135 11 151 368 113 348 7.01e-18 86.3
MsaG043876 MtrunA17_Chr3g0079691 40.217 92 48 2 296 387 493 577 3.14e-14 75.1
MsaG043876 MtrunA17_Chr7g0249301 48.837 86 42 2 288 373 52 135 7.92e-18 84.7
MsaG043876 MtrunA17_Chr7g0249301 51.562 64 30 1 296 359 290 352 4.13e-13 70.5
MsaG043876 MtrunA17_Chr7g0228941 51.471 68 33 0 299 366 53 120 1.75e-17 78.6
MsaG043876 MtrunA17_Chr8g0379461 55.556 63 28 0 299 361 77 139 3.89e-17 80.9
MsaG043876 MtrunA17_Chr4g0071521 49.398 83 41 1 273 355 221 302 5.56e-17 81.6
MsaG043876 MtrunA17_Chr7g0218081 45.745 94 44 3 294 382 224 315 5.71e-17 83.2
MsaG043876 MtrunA17_Chr7g0218081 49.333 75 36 2 298 372 410 482 4.47e-15 77.4
MsaG043876 MtrunA17_Chr7g0263531 43.750 96 52 2 282 377 173 266 8.55e-17 82.4
MsaG043876 MtrunA17_Chr7g0263531 40.196 102 44 3 275 361 324 423 2.13e-14 74.7
MsaG043876 MtrunA17_Chr5g0447701 56.522 69 26 3 294 361 88 153 1.30e-16 81.3
MsaG043876 MtrunA17_Chr5g0447701 50.000 60 29 1 299 358 253 311 1.17e-11 66.2
MsaG043876 MtrunA17_Chr1g0147881 48.193 83 42 1 273 355 238 319 1.69e-16 80.5
MsaG043876 MtrunA17_Chr7g0243891 53.425 73 32 2 290 362 208 278 4.29e-16 80.5
MsaG043876 MtrunA17_Chr7g0243891 44.304 79 35 2 298 368 374 451 4.47e-12 68.2
MsaG043876 MtrunA17_Chr7g0270051 49.296 71 36 0 288 358 43 113 5.09e-16 77.8
MsaG043876 MtrunA17_Chr4g0074001 42.697 89 38 2 270 358 343 418 7.96e-16 79.7
MsaG043876 MtrunA17_Chr3g0106491 47.917 96 36 5 275 363 174 262 6.96e-15 76.6
MsaG043876 MtrunA17_Chr3g0106491 46.875 64 33 1 296 359 363 425 1.92e-11 65.9
MsaG043876 MtrunA17_Chr7g0266611 43.158 95 52 1 353 445 2 96 1.66e-14 71.2
MsaG043876 MtrunA17_Chr3g0101131 45.161 93 43 3 276 362 215 305 2.08e-14 75.5
MsaG043876 MtrunA17_Chr3g0101131 46.269 67 35 1 298 364 415 480 5.60e-13 70.9
MsaG043876 MtrunA17_Chr4g0042831 50.746 67 32 1 298 364 278 343 2.20e-14 74.7
MsaG043876 MtrunA17_Chr4g0042831 47.143 70 36 1 289 358 136 204 7.19e-14 72.8
MsaG043876 MtrunA17_Chr5g0418191 49.254 67 33 1 298 364 320 385 1.52e-13 72.4
MsaG043876 MtrunA17_Chr5g0418191 47.368 76 38 2 289 364 142 215 4.94e-13 70.5
MsaG043876 MtrunA17_Chr5g0443131 48.485 66 33 1 298 363 157 221 1.76e-13 71.2
MsaG043876 MtrunA17_Chr4g0004911 48.438 64 32 1 295 358 181 243 3.36e-13 71.2
MsaG043876 MtrunA17_Chr4g0004911 47.619 63 32 1 296 358 334 395 5.96e-12 67.4
MsaG043876 MtrunA17_Chr2g0294231 47.692 65 33 1 298 362 203 266 3.88e-13 70.9
MsaG043876 MtrunA17_Chr5g0445311 48.387 62 31 1 298 359 364 424 3.89e-13 71.2
MsaG043876 MtrunA17_Chr5g0445311 29.218 243 127 12 143 358 34 258 6.85e-13 70.5
MsaG043876 MtrunA17_Chr3g0128671 41.489 94 46 3 262 353 98 184 3.95e-13 70.1
MsaG043876 MtrunA17_Chr7g0238921 44.737 76 41 1 280 355 106 180 3.98e-13 70.1
MsaG043876 MtrunA17_Chr8g0387981 40.230 87 46 2 273 353 103 189 4.37e-13 70.5
MsaG043876 MtrunA17_Chr2g0302391 46.667 60 32 0 299 358 212 271 6.79e-13 70.1
MsaG043876 MtrunA17_Chr5g0432161 51.562 64 29 2 295 358 241 302 7.70e-13 70.5
MsaG043876 MtrunA17_Chr5g0432161 48.438 64 32 1 296 359 419 481 2.54e-12 68.9
MsaG043876 MtrunA17_Chr3g0099641 33.557 149 83 6 213 358 176 311 8.57e-13 70.1
MsaG043876 MtrunA17_Chr8g0347251 40.404 99 49 3 265 353 95 193 8.86e-13 69.3
MsaG043876 MtrunA17_Chr8g0335811 44.928 69 36 2 296 364 152 218 1.62e-12 67.0
MsaG043876 MtrunA17_Chr7g0270191 44.156 77 42 1 298 374 145 220 1.65e-12 67.8
MsaG043876 MtrunA17_Chr3g0126391 46.552 58 29 1 298 353 133 190 1.84e-12 68.2
MsaG043876 MtrunA17_Chr4g0051881 50.000 58 28 1 298 355 178 234 2.08e-12 68.2
MsaG043876 MtrunA17_Chr3g0129321 44.776 67 34 3 295 360 115 179 2.83e-12 65.9
MsaG043876 MtrunA17_Chr1g0194481 37.079 89 54 2 278 366 139 225 3.82e-12 67.0
MsaG043876 MtrunA17_Chr8g0383351 42.424 66 36 2 296 361 158 221 9.60e-12 64.7
MsaG043876 MtrunA17_Chr6g0485431 44.928 69 35 3 291 358 148 214 1.50e-11 65.9
MsaG043876 MtrunA17_Chr7g0270831 45.614 57 31 0 299 355 217 273 1.55e-11 65.9
MsaG043876 MtrunA17_Chr5g0418781 34.513 113 71 3 265 377 93 202 1.70e-11 64.7
MsaG043876 MtrunA17_Chr8g0383251 30.827 133 80 6 236 358 37 167 1.75e-11 63.2
MsaG043876 MtrunA17_Chr2g0333851 40.278 72 38 1 296 362 167 238 2.12e-11 64.7
MsaG043876 MtrunA17_Chr2g0288871 41.667 72 41 1 284 355 292 362 2.44e-11 65.9
MsaG043876 MtrunA17_Chr3g0129361 43.077 65 35 2 296 360 142 204 2.80e-11 63.2
MsaG043876 MtrunA17_Chr7g0224801 41.667 60 34 1 296 355 91 149 2.95e-11 62.0
MsaG043876 MtrunA17_Chr7g0248721 35.556 90 54 3 268 355 109 196 3.41e-11 62.8
MsaG043876 MtrunA17_Chr1g0150271 41.270 63 37 0 296 358 99 161 3.85e-11 63.2
MsaG043876 MtrunA17_Chr5g0428351 50.000 60 28 1 299 356 184 243 5.59e-11 63.5
MsaG043876 MtrunA17_Chr4g0068541 42.623 61 34 1 298 358 208 267 6.08e-11 63.9
MsaG043876 MtrunA17_Chr5g0432071 36.634 101 50 5 274 372 87 175 7.04e-11 61.6
MsaG043876 MtrunA17_Chr4g0025061 45.902 61 33 0 299 359 361 421 8.64e-11 63.9
MsaG043876 MtrunA17_Chr4g0001361 41.270 63 37 0 296 358 160 222 9.67e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043876 AT4G01250.1 36.500 200 107 5 172 370 19 199 9.39e-25 103
MsaG043876 AT5G52830.1 53.125 96 43 2 275 370 143 236 1.01e-23 102
MsaG043876 AT4G23550.1 51.685 89 42 1 282 370 124 211 7.48e-23 99.0
MsaG043876 AT5G45050.4 51.282 78 38 0 278 355 1021 1098 1.24e-19 92.8
MsaG043876 AT5G45050.1 51.282 78 38 0 278 355 1160 1237 1.45e-19 92.4
MsaG043876 AT5G45050.2 51.282 78 38 0 278 355 1132 1209 1.72e-19 92.0
MsaG043876 AT4G24240.1 43.966 116 62 2 242 355 224 338 5.60e-19 88.6
MsaG043876 AT1G29280.1 61.667 60 23 0 299 358 75 134 1.59e-18 85.5
MsaG043876 AT4G31550.2 46.809 94 47 1 273 363 217 310 2.00e-18 86.3
MsaG043876 AT4G31550.1 58.462 65 27 0 299 363 247 311 2.68e-18 85.9
MsaG043876 AT2G23320.1 62.069 58 22 0 299 356 241 298 4.16e-18 85.5
MsaG043876 AT3G04670.1 62.712 59 22 0 299 357 263 321 7.35e-18 84.7
MsaG043876 AT2G34830.1 48.780 82 41 1 285 366 202 282 7.37e-18 85.9
MsaG043876 AT2G34830.2 48.780 82 41 1 285 366 211 291 8.19e-18 85.9
MsaG043876 AT2G24570.1 47.826 92 47 1 266 357 212 302 1.47e-17 84.0
MsaG043876 AT3G58710.1 52.000 75 29 1 299 366 72 146 1.68e-17 82.8
MsaG043876 AT5G28650.1 61.017 59 23 0 299 357 263 321 1.69e-17 83.6
MsaG043876 AT3G58710.2 52.000 75 29 1 299 366 71 145 1.71e-17 82.8
MsaG043876 AT3G01080.3 49.296 71 34 2 294 364 201 269 3.65e-17 82.8
MsaG043876 AT1G30650.1 48.780 82 41 1 285 366 204 284 4.10e-17 83.6
MsaG043876 AT3G01080.2 49.296 71 34 2 294 364 201 269 1.79e-16 81.6
MsaG043876 AT3G01080.2 50.820 61 29 1 298 358 344 403 1.69e-13 72.8
MsaG043876 AT3G01080.1 49.296 71 34 2 294 364 163 231 2.60e-16 81.3
MsaG043876 AT3G01080.1 50.820 61 29 1 298 358 306 365 2.55e-13 72.0
MsaG043876 AT2G37260.1 45.349 86 41 3 275 356 323 406 7.88e-16 79.7
MsaG043876 AT2G37260.1 50.746 67 31 2 293 359 160 224 7.35e-14 73.6
MsaG043876 AT2G30590.1 49.398 83 39 1 278 357 290 372 8.57e-16 79.3
MsaG043876 AT2G37260.2 45.349 86 41 3 275 356 241 324 1.20e-15 78.6
MsaG043876 AT2G37260.2 50.746 67 31 2 293 359 78 142 1.13e-13 72.4
MsaG043876 AT2G37260.3 45.349 86 41 3 275 356 243 326 1.21e-15 78.6
MsaG043876 AT2G37260.3 50.746 67 31 2 293 359 80 144 1.14e-13 72.4
MsaG043876 AT4G26440.1 43.011 93 51 2 297 389 177 267 1.76e-15 79.0
MsaG043876 AT4G26440.1 43.243 74 41 1 296 369 370 442 1.08e-12 70.5
MsaG043876 AT1G13960.1 48.649 74 36 2 294 367 225 296 2.95e-15 78.2
MsaG043876 AT1G13960.1 49.180 61 30 1 298 358 409 468 7.97e-13 70.9
MsaG043876 AT2G03340.1 50.704 71 33 2 293 363 245 313 4.35e-15 77.8
MsaG043876 AT2G03340.1 51.613 62 29 1 298 359 415 475 4.63e-14 74.7
MsaG043876 AT5G56270.1 52.941 68 30 2 297 364 272 337 5.46e-15 77.8
MsaG043876 AT5G56270.1 50.000 62 30 1 298 359 487 547 7.08e-13 71.2
MsaG043876 AT1G13960.2 47.368 76 38 2 294 369 72 145 6.50e-15 76.3
MsaG043876 AT1G13960.2 49.180 61 30 1 298 358 256 315 1.74e-12 68.9
MsaG043876 AT5G41570.1 40.000 85 48 2 273 355 71 154 8.48e-14 70.1
MsaG043876 AT5G07100.1 44.595 74 40 1 291 364 110 182 8.54e-14 72.4
MsaG043876 AT5G07100.1 42.254 71 40 1 296 366 232 301 1.92e-12 68.2
MsaG043876 AT2G38470.1 45.263 95 49 3 288 381 173 265 9.64e-14 73.6
MsaG043876 AT2G38470.1 50.000 62 30 1 298 359 362 422 4.26e-12 68.6
MsaG043876 AT1G64000.1 46.774 62 32 1 294 355 110 170 1.04e-13 70.1
MsaG043876 AT5G45260.1 45.946 74 37 2 299 370 1209 1281 1.14e-13 73.9
MsaG043876 AT1G66550.2 54.386 57 24 1 299 353 104 160 1.63e-13 70.5
MsaG043876 AT1G66550.1 54.386 57 24 1 299 353 109 165 1.80e-13 70.5
MsaG043876 AT5G07100.2 45.833 72 38 1 293 364 19 89 2.25e-13 69.7
MsaG043876 AT5G07100.2 42.254 71 40 1 296 366 139 208 7.66e-12 65.1
MsaG043876 AT5G07100.4 45.833 72 38 1 293 364 19 89 2.25e-13 69.7
MsaG043876 AT5G07100.4 42.254 71 40 1 296 366 139 208 7.66e-12 65.1
MsaG043876 AT5G07100.5 45.833 72 38 1 293 364 19 89 2.25e-13 69.7
MsaG043876 AT5G07100.5 42.254 71 40 1 296 366 139 208 7.66e-12 65.1
MsaG043876 AT5G07100.3 45.833 72 38 1 293 364 19 89 2.25e-13 69.7
MsaG043876 AT5G07100.3 42.254 71 40 1 296 366 139 208 7.66e-12 65.1
MsaG043876 AT4G30935.1 43.056 72 40 1 287 358 320 390 2.43e-13 72.0
MsaG043876 AT2G47260.1 41.176 85 48 2 278 362 155 237 5.83e-13 70.1
MsaG043876 AT5G43290.1 50.794 63 30 1 293 355 109 170 5.92e-13 69.3
MsaG043876 AT2G46130.1 46.667 60 31 1 296 355 28 86 7.21e-13 65.5
MsaG043876 AT5G22570.1 53.226 62 27 1 294 353 106 167 1.20e-12 68.6
MsaG043876 AT2G30250.1 48.485 66 33 1 299 364 329 393 1.52e-12 69.3
MsaG043876 AT2G30250.1 45.455 77 39 3 282 358 151 224 1.77e-12 69.3
MsaG043876 AT2G46400.1 39.604 101 55 3 262 356 64 164 1.64e-12 68.6
MsaG043876 AT2G04880.1 47.692 65 32 2 294 358 107 169 2.56e-12 68.9
MsaG043876 AT2G04880.1 45.205 73 36 2 299 368 308 379 5.12e-12 68.2
MsaG043876 AT2G04880.2 47.692 65 32 2 294 358 107 169 2.82e-12 68.9
MsaG043876 AT2G04880.2 45.205 73 36 2 299 368 284 355 5.07e-12 68.2
MsaG043876 AT2G44745.1 46.667 60 31 1 296 355 143 201 3.10e-12 66.2
MsaG043876 AT1G69310.4 38.298 94 56 2 273 366 123 214 3.30e-12 67.4
MsaG043876 AT1G69310.2 38.298 94 56 2 273 366 123 214 3.30e-12 67.4
MsaG043876 AT1G69310.1 38.298 94 56 2 273 366 123 214 3.30e-12 67.4
MsaG043876 AT4G11070.2 43.158 95 46 4 266 353 73 166 3.31e-12 67.4
MsaG043876 AT4G11070.1 43.158 95 46 4 266 353 105 198 4.03e-12 67.4
MsaG043876 AT2G21900.1 48.387 62 31 1 298 359 109 169 4.20e-12 65.5
MsaG043876 AT4G26640.7 36.893 103 62 3 296 397 169 269 4.76e-12 68.2
MsaG043876 AT4G26640.7 39.506 81 44 2 296 376 339 414 1.44e-11 66.6
MsaG043876 AT5G64810.1 47.761 67 30 3 296 360 108 171 4.77e-12 65.1
MsaG043876 AT4G26640.2 36.893 103 62 3 296 397 209 309 5.85e-12 68.2
MsaG043876 AT4G26640.2 39.506 81 44 2 296 376 379 454 1.79e-11 66.6
MsaG043876 AT4G26640.1 36.893 103 62 3 296 397 137 237 6.48e-12 67.8
MsaG043876 AT4G26640.1 39.506 81 44 2 296 376 307 382 2.09e-11 66.2
MsaG043876 AT4G18170.1 40.698 86 49 2 278 363 153 236 7.34e-12 66.6
MsaG043876 AT4G23810.1 44.828 87 40 4 274 353 130 215 7.61e-12 66.6
MsaG043876 AT3G01970.1 41.096 73 40 2 296 366 63 134 7.95e-12 63.5
MsaG043876 AT5G49520.1 44.118 68 37 1 298 365 221 287 8.52e-12 67.0
MsaG043876 AT4G39410.1 40.506 79 45 2 296 374 221 297 9.27e-12 66.2
MsaG043876 AT5G01900.1 39.773 88 50 2 269 353 80 167 1.13e-11 65.5
MsaG043876 AT1G55600.1 48.333 60 30 1 299 358 308 366 1.14e-11 67.0
MsaG043876 AT4G26640.5 36.893 103 62 3 296 397 33 133 1.21e-11 66.6
MsaG043876 AT4G26640.5 40.506 79 42 2 298 376 205 278 2.77e-11 65.5
MsaG043876 AT4G26640.6 36.893 103 62 3 296 397 33 133 1.21e-11 66.6
MsaG043876 AT4G26640.6 40.506 79 42 2 298 376 205 278 2.77e-11 65.5
MsaG043876 AT4G26640.4 36.893 103 62 3 296 397 33 133 1.21e-11 66.6
MsaG043876 AT4G26640.4 40.506 79 42 2 298 376 205 278 2.77e-11 65.5
MsaG043876 AT4G26640.3 36.893 103 62 3 296 397 33 133 1.21e-11 66.6
MsaG043876 AT4G26640.3 40.506 79 42 2 298 376 205 278 2.77e-11 65.5
MsaG043876 AT5G26170.1 47.541 61 31 1 295 355 110 169 1.38e-11 63.5
MsaG043876 AT1G66560.1 50.000 58 27 1 298 353 103 160 1.48e-11 64.7
MsaG043876 AT5G46350.1 39.759 83 48 2 284 366 170 250 1.69e-11 65.5
MsaG043876 AT1G80590.1 49.206 63 30 1 299 359 86 148 1.72e-11 64.3
MsaG043876 AT2G40740.1 38.043 92 52 2 267 353 139 230 2.36e-11 64.7
MsaG043876 AT2G40740.3 38.043 92 52 2 267 353 148 239 2.55e-11 64.7
MsaG043876 AT4G22070.4 41.667 72 41 1 284 355 284 354 2.64e-11 65.9
MsaG043876 AT4G22070.3 41.667 72 41 1 284 355 284 354 2.64e-11 65.9
MsaG043876 AT4G22070.2 41.667 72 41 1 284 355 284 354 2.64e-11 65.9
MsaG043876 AT4G22070.1 41.667 72 41 1 284 355 284 354 2.64e-11 65.9
MsaG043876 AT4G04450.2 45.614 57 31 0 299 355 199 255 3.14e-11 65.5
MsaG043876 AT1G66600.1 48.276 58 28 1 298 353 103 160 3.58e-11 63.5
MsaG043876 AT2G25000.2 36.145 83 48 1 281 358 81 163 3.62e-11 63.5
MsaG043876 AT2G25000.4 36.145 83 48 1 281 358 81 163 3.62e-11 63.5
MsaG043876 AT1G62300.1 45.614 57 31 0 299 355 313 369 3.64e-11 65.5
MsaG043876 AT2G25000.3 36.145 83 48 1 281 358 103 185 4.12e-11 63.5
MsaG043876 AT1G29860.1 43.478 69 38 1 298 366 136 203 4.32e-11 63.9
MsaG043876 AT5G24110.1 37.975 79 47 1 284 360 99 177 5.91e-11 63.9
MsaG043876 AT4G04450.1 45.614 57 31 0 299 355 293 349 7.24e-11 64.7
MsaG043876 AT2G25000.1 36.145 83 48 1 281 358 124 206 9.85e-11 62.8

Find 124 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCCAATTCTTCAATATTT+TGG 0.043748 8:-52543486 None:intergenic
GTCGATTTCACGTCGAAATT+TGG 0.080896 8:-52541907 None:intergenic
TTGGGTTTAAGTGGGGTAAT+TGG 0.221220 8:-52541303 None:intergenic
TGGATGTGAGGACCCTTTAA+TGG 0.244920 8:-52542281 None:intergenic
TCGATTTCACGTCGAAATTT+GGG 0.247314 8:-52541906 None:intergenic
TGGGGATTGTAGTATTATTC+TGG 0.273669 8:-52541887 None:intergenic
TCCCCAAAATATTGAAGAAT+TGG 0.278213 8:+52543483 MsaT043876.1:CDS
TGGGTTTAAGTGGGGTAATT+GGG 0.291623 8:-52541302 None:intergenic
CTTCATTGAACTAGACAAAT+TGG 0.302614 8:+52541329 MsaT043876.1:CDS
CTAGTTTGTGGTAGTTGAAT+TGG 0.323626 8:-52541975 None:intergenic
CAAAATTTAAATCAGGAAAA+AGG 0.334262 8:+52542033 MsaT043876.1:CDS
TCATGGAGATGGAGAAAATA+TGG 0.338703 8:+52542250 MsaT043876.1:CDS
GGTGGAGATGGGGATAGTGG+TGG 0.349627 8:-52541646 None:intergenic
GATTGAAGGGGTTAAGATAT+TGG 0.362111 8:-52541397 None:intergenic
ATGATTAACTCTAGTGCTAA+TGG 0.366249 8:+52541798 MsaT043876.1:CDS
GGAGATGGGGATAGTGGTGG+GGG 0.368068 8:-52541643 None:intergenic
TTATAGGACTAGTGGTGGTG+GGG 0.369329 8:-52541758 None:intergenic
GTCTAGTTCAATGAAGTCAT+TGG 0.370315 8:-52541322 None:intergenic
ATGGAGTTCCGATTATTTGC+TGG 0.373756 8:-52543241 None:intergenic
GTTGTGGTGGTAGGGATTAT+AGG 0.375777 8:-52541774 None:intergenic
ATGAAGTCATTGGGTTTAAG+TGG 0.379299 8:-52541312 None:intergenic
ATAAGGTTTCAGAGGATTCA+TGG 0.385027 8:+52542233 MsaT043876.1:CDS
GGTCAACGCGGTGGGGATAA+TGG 0.386003 8:-52541427 None:intergenic
GGATGTGAGGACCCTTTAAT+GGG 0.387521 8:-52542280 None:intergenic
GTAGTTGATTGTGACTTCTC+TGG 0.387814 8:-52541953 None:intergenic
ACCCGAGCCTTGCTGCTCTC+TGG 0.394496 8:-52543385 None:intergenic
GTTAAGATATTGGTGGTAGT+AGG 0.395052 8:-52541387 None:intergenic
TTAAGATATTGGTGGTAGTA+GGG 0.400343 8:-52541386 None:intergenic
AGCTGCAACATTACCGCATT+AGG 0.400572 8:-52543355 None:intergenic
ACAATAAACCAGCAAATAAT+CGG 0.401514 8:+52543233 MsaT043876.1:CDS
GTGGAGATGGGGATAGTGGT+GGG 0.402347 8:-52541645 None:intergenic
GATTATAGGACTAGTGGTGG+TGG 0.413064 8:-52541760 None:intergenic
GGTAGTAGGGGTAGTGGAGT+TGG 0.420421 8:-52541679 None:intergenic
GCCAGAGAGCAGCAAGGCTC+GGG 0.426732 8:+52543384 MsaT043876.1:CDS
GGAATAGTGATGGTGGAGAT+GGG 0.428803 8:-52541657 None:intergenic
AAAGGTGATGGCAGTGGTGA+TGG 0.430730 8:-52541709 None:intergenic
AAATGATAATCAAGTATCAT+TGG 0.431401 8:+52542192 MsaT043876.1:CDS
AAACGACCAAAATTTAAATC+AGG 0.434297 8:+52542026 MsaT043876.1:CDS
GGGAATAGTGATGGTGGAGA+TGG 0.435104 8:-52541658 None:intergenic
AAGTTTGTATCAGCTGTTGA+TGG 0.435333 8:+52543292 MsaT043876.1:CDS
GATGGTGGAGATGGGGATAG+TGG 0.437402 8:-52541649 None:intergenic
AAGGTGATGGCAGTGGTGAT+GGG 0.441027 8:-52541708 None:intergenic
TAATCATGGTTGTGGTGGTA+GGG 0.441097 8:-52541782 None:intergenic
ATAATGGTGGTTGTGATTGA+AGG 0.441781 8:-52541411 None:intergenic
GTAGTAGGGGTAGTGGAGTT+GGG 0.441918 8:-52541678 None:intergenic
ACTTATGAAGTGACATATCT+AGG 0.442305 8:+52543199 MsaT043876.1:CDS
TTTCACAAACATCATAAACT+TGG 0.449071 8:+52543327 MsaT043876.1:CDS
TCTAGTTCAATGAAGTCATT+GGG 0.450061 8:-52541321 None:intergenic
TGACTTCTCTGGAGTTGTTG+TGG 0.463915 8:-52541942 None:intergenic
ATTACCTGCCGAACAACAAC+AGG 0.466894 8:+52541854 MsaT043876.1:CDS
GGCTTGAACCTGTTGTTGTT+CGG 0.469350 8:-52541862 None:intergenic
AGCCAGAGAGCAGCAAGGCT+CGG 0.475353 8:+52543383 MsaT043876.1:CDS
GGTAATGGTAGTAGGGGTAG+TGG 0.478553 8:-52541685 None:intergenic
GATTGTAGTATTATTCTGGT+TGG 0.480965 8:-52541883 None:intergenic
GTATCATTGGTGAGCTATGT+GGG 0.490327 8:+52542205 MsaT043876.1:CDS
GGTGGGGAAAATGGTTGTGG+TGG 0.491264 8:-52541448 None:intergenic
TGAACCTGTTGTTGTTCGGC+AGG 0.492305 8:-52541858 None:intergenic
GTTGTGGTGGTCAACGCGGT+GGG 0.494214 8:-52541435 None:intergenic
GTTGTTGTTCAACGTGTGTT+GGG 0.495648 8:-52541566 None:intergenic
ATTATAGGACTAGTGGTGGT+GGG 0.498019 8:-52541759 None:intergenic
TTAATCATGGTTGTGGTGGT+AGG 0.500138 8:-52541783 None:intergenic
GTTGTTGTGGAACTAATTCA+TGG 0.500678 8:-52541929 None:intergenic
TATAGGACTAGTGGTGGTGG+GGG 0.501201 8:-52541757 None:intergenic
ACTTAGTCCTTTACCTACAC+TGG 0.501454 8:+52543447 MsaT043876.1:CDS
AGCAAGGCTCGGGTTCTTGT+CGG 0.505919 8:+52543394 MsaT043876.1:CDS
TAATGGTGGTTGTGATTGAA+GGG 0.518016 8:-52541410 None:intergenic
ATTAGCACTAGAGTTAATCA+TGG 0.521704 8:-52541796 None:intergenic
TGGAGATGGGGATAGTGGTG+GGG 0.522513 8:-52541644 None:intergenic
TGAAGTCATTGGGTTTAAGT+GGG 0.523364 8:-52541311 None:intergenic
TCTAGCTCAGTGAACTCACT+TGG 0.527705 8:-52541597 None:intergenic
TTCACAAACATCATAAACTT+GGG 0.528355 8:+52543328 MsaT043876.1:CDS
GCTTTGCCCAGTGTAGGTAA+AGG 0.528650 8:-52543454 None:intergenic
TGTTGTTGTTCAACGTGTGT+TGG 0.529404 8:-52541567 None:intergenic
GAGATGGGGATAGTGGTGGG+GGG 0.531742 8:-52541642 None:intergenic
TTTCAGAGGATTCATGGAGA+TGG 0.534538 8:+52542239 MsaT043876.1:CDS
TCAACAGCTGATACAAACTT+TGG 0.537334 8:-52543289 None:intergenic
GGGGTGTTGATAGTTGTTGG+GGG 0.540872 8:-52541738 None:intergenic
CTTAGTCCTTTACCTACACT+GGG 0.545206 8:+52543448 MsaT043876.1:CDS
CGATTTCACGTCGAAATTTG+GGG 0.547197 8:-52541905 None:intergenic
GGTTGTGGTGGTCAACGCGG+TGG 0.551592 8:-52541436 None:intergenic
ACTAGAGTTAATCATGGTTG+TGG 0.553627 8:-52541790 None:intergenic
AGTAGGTGTAGTAATGGTCG+AGG 0.558267 8:-52541472 None:intergenic
AAAGATCTAAAACTAGTTTG+TGG 0.559260 8:-52541987 None:intergenic
AGTATCATTGGTGAGCTATG+TGG 0.560291 8:+52542204 MsaT043876.1:CDS
TAAGATATTGGTGGTAGTAG+GGG 0.560554 8:-52541385 None:intergenic
GGTGATGGCAGTGGTGATGG+GGG 0.562066 8:-52541706 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.562993 8:-52541505 None:intergenic
AGGTGATGGCAGTGGTGATG+GGG 0.563860 8:-52541707 None:intergenic
TGAAGGGGTTAAGATATTGG+TGG 0.566707 8:-52541394 None:intergenic
GTAAAATCATCATAGCAATA+CGG 0.572394 8:-52541255 None:intergenic
GATATTGGTGGTAGTAGGGG+TGG 0.572507 8:-52541382 None:intergenic
TTAAAGGGTCCTCACATCCA+AGG 0.572922 8:+52542284 MsaT043876.1:CDS
TTGTTTAGCAAGAAAACAAG+TGG 0.573603 8:+52543153 MsaT043876.1:CDS
ATATTGGTGGTAGTAGGGGT+GGG 0.578232 8:-52541381 None:intergenic
AGTGGAGTTGGGAATAGTGA+TGG 0.587083 8:-52541667 None:intergenic
GAATAGTGATGGTGGAGATG+GGG 0.587695 8:-52541656 None:intergenic
TATTGGTGGTAGTAGGGGTG+GGG 0.595619 8:-52541380 None:intergenic
AATGAGAAATCAGAAACGGC+GGG 0.597213 8:-52541834 None:intergenic
GGAGTTGGGAATAGTGATGG+TGG 0.605137 8:-52541664 None:intergenic
ATGAAAACTAGTGTTAGTGA+TGG 0.606015 8:-52541511 None:intergenic
GATATGAGTAGGTGTAGTAA+TGG 0.607202 8:-52541478 None:intergenic
GTATTGGTGGTGATATGAGT+AGG 0.607640 8:-52541489 None:intergenic
TGTACTAAAGGTGATGGCAG+TGG 0.608456 8:-52541715 None:intergenic
AGTTCACTGAGCTAGAACAA+TGG 0.612785 8:+52541604 MsaT043876.1:CDS
AGTGTTAGTGATGGTATTGG+TGG 0.615093 8:-52541502 None:intergenic
AGAGTTAATCATGGTTGTGG+TGG 0.615137 8:-52541787 None:intergenic
ACTCCATTGTCGATAGCTCT+CGG 0.621472 8:+52543257 MsaT043876.1:CDS
GTAGGTGTAGTAATGGTCGA+GGG 0.624066 8:-52541471 None:intergenic
AGGTAATGAGAAATCAGAAA+CGG 0.625218 8:-52541838 None:intergenic
GAAGTCATTGGGTTTAAGTG+GGG 0.626876 8:-52541310 None:intergenic
AATGGTGGTTGTGATTGAAG+GGG 0.628525 8:-52541409 None:intergenic
AAACTTGGGTTCTCCTAATG+CGG 0.633652 8:+52543342 MsaT043876.1:CDS
GTACCGAGAGCTATCGACAA+TGG 0.639321 8:-52543260 None:intergenic
GGTAGGGATTATAGGACTAG+TGG 0.644053 8:-52541766 None:intergenic
TTGTGGTGGTCAACGCGGTG+GGG 0.645732 8:-52541434 None:intergenic
CAACGCGGTGGGGATAATGG+TGG 0.646185 8:-52541424 None:intergenic
TATCATTGGTGAGCTATGTG+GGG 0.653483 8:+52542206 MsaT043876.1:CDS
TAATGAGAAATCAGAAACGG+CGG 0.658115 8:-52541835 None:intergenic
ATCATTGGTGAGCTATGTGG+GGG 0.659269 8:+52542207 MsaT043876.1:CDS
TAGGTGTAGTAATGGTCGAG+GGG 0.662277 8:-52541470 None:intergenic
AGGGATTATAGGACTAGTGG+TGG 0.676672 8:-52541763 None:intergenic
TGATCAGCCAGAGAGCAGCA+AGG 0.680979 8:+52543378 MsaT043876.1:CDS
AATGGTTGTGGTGGTCAACG+CGG 0.682395 8:-52541439 None:intergenic
AGGTGTAGTAATGGTCGAGG+GGG 0.695973 8:-52541469 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 52541219 52543510 52541219 ID=MsaG043876
Chr8 mRNA 52541219 52543510 52541219 ID=MsaT043876.1;Parent=MsaG043876
Chr8 exon 52541219 52542054 52541219 ID=MsaT043876.1.exon1;Parent=MsaT043876.1
Chr8 CDS 52541219 52542054 52541219 ID=cds.MsaT043876.1;Parent=MsaT043876.1
Chr8 exon 52542189 52542305 52542189 ID=MsaT043876.1.exon2;Parent=MsaT043876.1
Chr8 CDS 52542189 52542305 52542189 ID=cds.MsaT043876.1;Parent=MsaT043876.1
Chr8 exon 52543123 52543510 52543123 ID=MsaT043876.1.exon3;Parent=MsaT043876.1
Chr8 CDS 52543123 52543510 52543123 ID=cds.MsaT043876.1;Parent=MsaT043876.1
Gene Sequence

>MsaT043876.1

ATGAAAAATAACATCACATCCCCTCAAAACACAACACCGTATTGCTATGATGATTTTACATTTACCCAAGAAAATAGCACAATCCCAATTACCCCACTTAAACCCAATGACTTCATTGAACTAGACAAATTGGTAATTCGTTCCAATCCAACAAAAATTATCCCCACCCCTACTACCACCAATATCTTAACCCCTTCAATCACAACCACCATTATCCCCACCGCGTTGACCACCACAACCATTTTCCCCACCCCCTCGACCATTACTACACCTACTCATATCACCACCAATACCATCACTAACACTAGTTTTCATGGATCTGATCAAAATTCTACATTTTCTTATTTCCCAACACACGTTGAACAACAACATATGCAACCAAGTGAGTTCACTGAGCTAGAACAATGGATGCTCCAATTCAATCCCCCCACCACTATCCCCATCTCCACCATCACTATTCCCAACTCCACTACCCCTACTACCATTACCCCCATCACCACTGCCATCACCTTTAGTACACCCCCAACAACTATCAACACCCCCACCACCACTAGTCCTATAATCCCTACCACCACAACCATGATTAACTCTAGTGCTAATGGACTCAATCAATATCCCGCCGTTTCTGATTTCTCATTACCTGCCGAACAACAACAGGTTCAAGCCAACCAGAATAATACTACAATCCCCAAATTTCGACGTGAAATCGACCATGAATTAGTTCCACAACAACTCCAGAGAAGTCACAATCAACTACCAATTCAACTACCACAAACTAGTTTTAGATCTTTACCAAATACAAAATCAAAACGACCAAAATTTAAATCAGGAAAAAGAAAAAATGATAATCAAGTATCATTGGTGAGCTATGTGGGGGCAGATAAGGTTTCAGAGGATTCATGGAGATGGAGAAAATATGGACAAAAACCCATTAAAGGGTCCTCACATCCAAGAAGCTACTACAAATGTAGCAGTTCCAGTGATTGTTTAGCAAGAAAACAAGTGGAAAAAATAAAAGATGGAGATGACACTTATGAAGTGACATATCTAGGAGAACACAATCACAATAAACCAGCAAATAATCGGAACTCCATTGTCGATAGCTCTCGGTACATGTCACCAAAGTTTGTATCAGCTGTTGATGGAGAAGCTAGTTGTTTCACAAACATCATAAACTTGGGTTCTCCTAATGCGGTAATGTTGCAGCTTGATCAGCCAGAGAGCAGCAAGGCTCGGGTTCTTGTCGGTCAATCAAAAATCCCTGATCTAAAAACTGAACTTAGTCCTTTACCTACACTGGGCAAAGCAAAATCTCCCCAAAATATTGAAGAATTGGATTGA
Protein sequence

>MsaT043876.1

MKNNITSPQNTTPYCYDDFTFTQENSTIPITPLKPNDFIELDKLVIRSNPTKIIPTPTTTNILTPSITTTIIPTALTTTTIFPTPSTITTPTHITTNTITNTSFHGSDQNSTFSYFPTHVEQQHMQPSEFTELEQWMLQFNPPTTIPISTITIPNSTTPTTITPITTAITFSTPPTTINTPTTTSPIIPTTTTMINSSANGLNQYPAVSDFSLPAEQQQVQANQNNTTIPKFRREIDHELVPQQLQRSHNQLPIQLPQTSFRSLPNTKSKRPKFKSGKRKNDNQVSLVSYVGADKVSEDSWRWRKYGQKPIKGSSHPRSYYKCSSSSDCLARKQVEKIKDGDDTYEVTYLGEHNHNKPANNRNSIVDSSRYMSPKFVSAVDGEASCFTNIINLGSPNAVMLQLDQPESSKARVLVGQSKIPDLKTELSPLPTLGKAKSPQNIEELD*