AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043879


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043879 MtrunA17_Chr8g0367691 94.492 236 13 0 12 247 33 268 1.50e-161 448
MsaG043879 MtrunA17_Chr2g0315941 91.983 237 18 1 12 247 32 268 2.83e-154 429
MsaG043879 MtrunA17_Chr5g0412501 46.602 206 101 2 43 246 73 271 3.99e-55 178
MsaG043879 MtrunA17_Chr1g0188441 39.614 207 90 4 47 246 8 186 2.32e-43 145
MsaG043879 MtrunA17_Chr4g0076821 37.395 238 108 6 47 246 112 346 1.64e-42 148
MsaG043879 MtrunA17_Chr3g0133181 44.000 200 101 3 47 243 79 270 1.26e-41 143
MsaG043879 MtrunA17_Chr5g0404271 38.537 205 106 4 48 243 99 292 3.70e-41 142
MsaG043879 MtrunA17_Chr5g0427051 36.614 254 127 7 18 246 23 267 6.31e-39 136
MsaG043879 MtrunA17_Chr7g0217231 39.109 202 113 4 48 246 88 282 1.96e-37 134
MsaG043879 MtrunA17_Chr6g0453231 41.089 202 108 5 48 246 91 284 1.76e-36 131
MsaG043879 MtrunA17_Chr8g0357721 40.000 170 53 4 57 221 14 139 8.80e-29 106
MsaG043879 MtrunA17_Chr8g0357741 58.763 97 31 5 155 246 4 96 7.41e-26 97.1
MsaG043879 MtrunA17_Chr3g0092371 62.745 51 19 0 47 97 19 69 7.41e-18 75.9
MsaG043879 MtrunA17_Chr8g0361671 57.143 56 24 0 185 240 272 327 3.74e-16 77.0
MsaG043879 MtrunA17_Chr8g0361671 63.462 52 17 1 48 97 64 115 1.39e-15 75.5
MsaG043879 MtrunA17_Chr8g0349871 54.717 53 24 0 182 234 11 63 2.22e-15 68.9
MsaG043879 MtrunA17_Chr6g0453221 58.000 50 17 1 47 96 20 65 2.15e-14 66.6
MsaG043879 MtrunA17_Chr6g0479901 45.902 61 32 1 183 242 100 160 1.13e-13 67.4
MsaG043879 MtrunA17_Chr6g0479901 40.909 66 38 1 48 112 19 84 1.73e-13 66.6
MsaG043879 MtrunA17_Chr4g0060731 52.542 59 25 1 39 97 16 71 1.55e-13 64.3
MsaG043879 MtrunA17_Chr8g0349881 61.364 44 17 0 182 225 35 78 2.75e-13 63.5
MsaG043879 MtrunA17_Chr8g0349921 59.091 44 18 0 182 225 35 78 3.82e-12 60.5
MsaG043879 MtrunA17_Chr7g0243651 60.000 40 16 0 47 86 2 41 5.82e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043879 AT4G24660.1 36.694 248 126 5 1 246 1 219 5.37e-47 155
MsaG043879 AT2G02540.2 40.976 205 110 4 47 246 86 284 1.02e-46 157
MsaG043879 AT2G02540.1 40.976 205 110 4 47 246 86 284 1.02e-46 157
MsaG043879 AT4G24660.2 36.327 245 125 5 1 243 1 216 1.49e-45 152
MsaG043879 AT5G65410.1 36.863 255 131 5 1 245 18 252 7.99e-45 152
MsaG043879 AT1G14440.1 38.462 208 92 5 47 240 89 274 1.21e-39 139
MsaG043879 AT1G14440.2 38.462 208 92 5 47 240 89 274 1.21e-39 139
MsaG043879 AT1G69600.1 39.706 204 100 6 48 245 31 217 2.01e-38 134
MsaG043879 AT2G18350.1 38.350 206 99 7 45 246 79 260 1.30e-36 130
MsaG043879 AT5G15210.1 37.073 205 92 5 48 240 56 235 4.17e-35 126
MsaG043879 AT3G50890.1 31.950 241 119 7 15 243 33 240 3.11e-31 115
MsaG043879 AT3G28920.1 35.945 217 101 7 48 243 52 251 5.20e-30 114
MsaG043879 AT5G60480.1 35.204 196 97 5 48 241 37 204 2.76e-27 105
MsaG043879 AT5G42780.1 32.468 231 126 5 18 243 33 238 6.84e-26 102
MsaG043879 AT1G74660.2 64.706 51 18 0 47 97 38 88 9.90e-22 86.7
MsaG043879 AT1G74660.1 64.706 51 18 0 47 97 38 88 1.75e-21 86.3
MsaG043879 AT1G75240.1 61.290 62 23 1 47 107 75 136 1.31e-20 89.0
MsaG043879 AT1G75240.1 61.667 60 23 0 181 240 237 296 2.79e-18 82.8
MsaG043879 AT3G28917.1 50.000 68 29 2 47 113 32 95 3.37e-17 75.1
MsaG043879 AT1G18835.1 58.824 51 18 1 47 97 25 72 2.02e-15 70.1
MsaG043879 AT5G39760.1 50.000 62 31 0 185 246 201 262 2.94e-15 74.3

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAGTCGGCTGAGGGAAAC+GGG 0.315201 8:+52582904 None:intergenic
AGCATGTGACTGGGACTTCT+AGG 0.330483 8:-52582847 MsaT043879.1:CDS
CACTTTCCAAGGGGTGATCT+TGG 0.335585 8:-52582779 MsaT043879.1:CDS
TTTCTCTCCGATGAAAATTT+CGG 0.335714 8:+52582972 None:intergenic
AGGGAGTCGGCTGAGGGAAA+CGG 0.388732 8:+52582903 None:intergenic
ATTGTTTCAACGTAACTAGT+TGG 0.415917 8:+52583190 None:intergenic
ACCGGTTATCAGCATGTGAC+TGG 0.421058 8:-52582857 MsaT043879.1:CDS
CCCAGTCACATGCTGATAAC+CGG 0.436811 8:+52582856 None:intergenic
TTCTTCATCATGATGATTCG+AGG 0.450499 8:+52583162 None:intergenic
TAAGTATCACCATGATAGAT+TGG 0.451827 8:+52582740 None:intergenic
GTCGAGAAGGGAGTCGGCTG+AGG 0.453909 8:+52582896 None:intergenic
ATTGGAGGCTACGCTCTTGA+TGG 0.454275 8:-52583067 MsaT043879.1:CDS
GGAGTCGGCTGAGGGAAACG+GGG 0.458116 8:+52582905 None:intergenic
TATCAAAAGGAAAGGATGCT+TGG 0.463362 8:-52582662 MsaT043879.1:CDS
AAGAATCACGCTTCCAGCAT+TGG 0.464419 8:-52583085 MsaT043879.1:CDS
CAAGTTCACTCATTATCAAA+AGG 0.480127 8:-52582675 MsaT043879.1:CDS
ATGATGAGGCCCACTTTCCA+AGG 0.481132 8:-52582790 MsaT043879.1:CDS
TATCATGGTGATACTTATAG+TGG 0.482130 8:-52582734 MsaT043879.1:CDS
TTCTTCAGTTGTTCATGATG+AGG 0.486893 8:-52582804 MsaT043879.1:CDS
TTTCTAGTGCTTATTTCCAA+GGG 0.501479 8:+52582517 None:intergenic
AAGAGCGTAGCCTCCAATGC+TGG 0.502391 8:+52583072 None:intergenic
GCATGTGACTGGGACTTCTA+GGG 0.503163 8:-52582846 MsaT043879.1:CDS
CAAGATCACCCCTTGGAAAG+TGG 0.509725 8:+52582780 None:intergenic
TATAACAACAAACACACCCT+TGG 0.522734 8:-52582533 MsaT043879.1:CDS
AATGGAAACAAAAGAAAATG+AGG 0.526367 8:-52583242 MsaT043879.1:CDS
AATCACGCTTCCAGCATTGG+AGG 0.528529 8:-52583082 MsaT043879.1:CDS
TCATCATGAACAACTGAAGA+AGG 0.530900 8:+52582806 None:intergenic
AAGATCACCCCTTGGAAAGT+GGG 0.533623 8:+52582781 None:intergenic
GGCTACGCTCTTGATGGCTG+TGG 0.535093 8:-52583061 MsaT043879.1:CDS
GAGTCGGCTGAGGGAAACGG+GGG 0.542032 8:+52582906 None:intergenic
CCAAGGGGTGATCTTGGAGA+AGG 0.555872 8:-52582773 MsaT043879.1:CDS
GATACTTATAGTGGTGGAGA+AGG 0.556865 8:-52582725 MsaT043879.1:CDS
TGATGAGGCCCACTTTCCAA+GGG 0.559791 8:-52582789 MsaT043879.1:CDS
CCGGTTATCAGCATGTGACT+GGG 0.570387 8:-52582856 MsaT043879.1:CDS
TCACTCATTATCAAAAGGAA+AGG 0.573122 8:-52582670 MsaT043879.1:CDS
TCTCAAAGCAATAACTTCGA+AGG 0.575954 8:-52583127 MsaT043879.1:CDS
TCGAGAAGGGAGTCGGCTGA+GGG 0.581880 8:+52582897 None:intergenic
ATGGTTCCTTCAATTCCAGC+GGG 0.587215 8:+52583028 None:intergenic
AAATGAGGAAGACGTAGTGA+TGG 0.593757 8:-52583227 MsaT043879.1:CDS
CATGGTGATACTTATAGTGG+TGG 0.595025 8:-52582731 MsaT043879.1:CDS
GTTGTTGAGCCAATCTATCA+TGG 0.597526 8:-52582749 MsaT043879.1:CDS
TCAGCCGACTCCCTTCTCGA+CGG 0.603425 8:-52582894 MsaT043879.1:CDS
CCTTCTCCAAGATCACCCCT+TGG 0.609902 8:+52582773 None:intergenic
AAAACCGTCGAGAAGGGAGT+CGG 0.612389 8:+52582890 None:intergenic
CATGTGACTGGGACTTCTAG+GGG 0.617828 8:-52582845 MsaT043879.1:CDS
ATACTTATAGTGGTGGAGAA+GGG 0.640736 8:-52582724 MsaT043879.1:CDS
GATGGTTCCTTCAATTCCAG+CGG 0.666037 8:+52583027 None:intergenic
GTCACATGCTGATAACCGGT+CGG 0.668109 8:+52582860 None:intergenic
AAGGGTCGTCAAAATCAAAG+AGG 0.684157 8:-52582706 MsaT043879.1:CDS
ATGTGACTGGGACTTCTAGG+GGG 0.700324 8:-52582844 MsaT043879.1:CDS
GATGAGGCCCACTTTCCAAG+GGG 0.725349 8:-52582788 MsaT043879.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 52582520 52583263 52582520 ID=MsaG043879
Chr8 mRNA 52582520 52583263 52582520 ID=MsaT043879.1;Parent=MsaG043879
Chr8 exon 52582520 52583263 52582520 ID=MsaT043879.1.exon1;Parent=MsaT043879.1
Chr8 CDS 52582520 52583263 52582520 ID=cds.MsaT043879.1;Parent=MsaT043879.1
Gene Sequence

>MsaT043879.1

ATGGAAACAAAAGAAAATGAGGAAGACGTAGTGATGGCTAATGACAGTGATCCAACTAGTTACGTTGAAACAATTAAAACCTCGAATCATCATGATGAAGAAACTCTAATGAATTCTCAAAGCAATAACTTCGAAGGAAAGTTCAAAGAGTGTAGAAAGAATCACGCTTCCAGCATTGGAGGCTACGCTCTTGATGGCTGTGGCGAGTTTTTACCCGCTGGAATTGAAGGAACCATCGAATTTTTCAAATGCGCTGCGTGTAACTGTCACCGAAATTTTCATCGGAGAGAAAATGGTGTTGTTAATGAAGAGAATATTTCCCTTCCTTTTAACAACCCCCGTTTCCCTCAGCCGACTCCCTTCTCGACGGTTTTTCAAACTCCGACCGGTTATCAGCATGTGACTGGGACTTCTAGGGGGACAACTACTTCTATTCCTTCTTCAGTTGTTCATGATGAGGCCCACTTTCCAAGGGGTGATCTTGGAGAAGGTGTTGTTGAGCCAATCTATCATGGTGATACTTATAGTGGTGGAGAAGGGTCGTCAAAATCAAAGAGGTTCAGATCCAAGTTCACTCATTATCAAAAGGAAAGGATGCTTGGTTTTGCTATGAAATCGGGTTGGAAGATTCCTAAGCAAGATGAAAATGTAGTTGAACAATTTTGTAATGAGATTGGTGTAAAGTGTAAAACTTTTAGGGTGTGGATGTATAACAACAAACACACCCTTGGAAATAAGCACTAG

Protein sequence

>MsaT043879.1

METKENEEDVVMANDSDPTSYVETIKTSNHHDEETLMNSQSNNFEGKFKECRKNHASSIGGYALDGCGEFLPAGIEGTIEFFKCAACNCHRNFHRRENGVVNEENISLPFNNPRFPQPTPFSTVFQTPTGYQHVTGTSRGTTTSIPSSVVHDEAHFPRGDLGEGVVEPIYHGDTYSGGEGSSKSKRFRSKFTHYQKERMLGFAMKSGWKIPKQDENVVEQFCNEIGVKCKTFRVWMYNNKHTLGNKH*