AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043889 MtrunA17_Chr2g0315941 50.202 247 99 5 1 234 32 267 1.54e-73 224
MsaG043889 MtrunA17_Chr8g0367691 48.583 247 102 6 1 234 33 267 9.41e-69 212
MsaG043889 MtrunA17_Chr5g0412501 46.047 215 84 5 36 235 75 272 9.46e-48 158
MsaG043889 MtrunA17_Chr5g0427051 40.840 262 111 6 3 235 22 268 1.50e-45 152
MsaG043889 MtrunA17_Chr3g0133181 43.333 210 87 5 37 231 78 270 9.55e-44 148
MsaG043889 MtrunA17_Chr5g0404271 35.407 209 110 3 39 234 99 295 1.60e-42 145
MsaG043889 MtrunA17_Chr1g0188441 38.208 212 85 6 37 234 7 186 7.96e-42 140
MsaG043889 MtrunA17_Chr6g0453231 39.336 211 96 5 39 234 91 284 4.49e-39 137
MsaG043889 MtrunA17_Chr8g0357721 47.853 163 47 5 48 209 14 139 1.66e-38 130
MsaG043889 MtrunA17_Chr4g0076821 35.656 244 99 7 39 234 113 346 3.94e-38 136
MsaG043889 MtrunA17_Chr7g0217231 38.208 212 102 5 37 234 86 282 7.88e-38 134
MsaG043889 MtrunA17_Chr8g0357741 60.577 104 31 3 132 234 2 96 9.17e-33 114
MsaG043889 MtrunA17_Chr8g0361671 53.968 63 29 0 172 234 271 333 7.37e-17 78.6
MsaG043889 MtrunA17_Chr8g0361671 55.932 59 24 1 32 88 57 115 1.92e-15 74.7
MsaG043889 MtrunA17_Chr3g0092371 50.000 68 31 2 37 102 18 84 1.66e-16 72.0
MsaG043889 MtrunA17_Chr8g0349871 61.538 52 20 0 173 224 14 65 1.85e-16 71.6
MsaG043889 MtrunA17_Chr6g0479901 46.667 60 31 1 172 230 101 160 1.27e-14 69.7
MsaG043889 MtrunA17_Chr6g0479901 41.538 65 37 1 39 102 19 83 6.31e-13 65.1
MsaG043889 MtrunA17_Chr8g0349881 68.293 41 13 0 173 213 38 78 1.95e-14 66.2
MsaG043889 MtrunA17_Chr8g0349921 67.500 40 13 0 174 213 39 78 4.48e-14 65.5
MsaG043889 MtrunA17_Chr6g0453221 51.562 64 27 2 37 100 19 78 1.16e-12 62.0
MsaG043889 MtrunA17_Chr4g0060731 44.262 61 31 1 30 90 16 73 8.93e-12 59.3
MsaG043889 MtrunA17_Chr7g0243651 58.537 41 17 0 37 77 1 41 2.05e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043889 AT4G24660.1 42.574 202 87 5 36 235 46 220 5.80e-49 160
MsaG043889 AT4G24660.2 41.414 198 87 5 36 231 46 216 8.48e-46 152
MsaG043889 AT5G65410.1 38.889 216 83 5 36 235 72 254 3.36e-43 147
MsaG043889 AT2G02540.2 36.530 219 99 5 37 234 85 284 1.22e-42 147
MsaG043889 AT2G02540.1 36.530 219 99 5 37 234 85 284 1.22e-42 147
MsaG043889 AT1G14440.1 38.000 200 115 3 37 234 88 280 3.04e-41 143
MsaG043889 AT1G14440.2 38.000 200 115 3 37 234 88 280 3.04e-41 143
MsaG043889 AT5G15210.1 38.756 209 113 4 22 228 40 235 2.60e-39 137
MsaG043889 AT1G69600.1 39.409 203 99 6 37 231 29 215 8.03e-38 132
MsaG043889 AT2G18350.1 39.109 202 100 4 36 234 79 260 1.30e-37 132
MsaG043889 AT3G50890.1 35.610 205 100 5 39 233 60 242 3.35e-35 125
MsaG043889 AT3G28920.1 34.862 218 105 5 36 231 49 251 2.55e-31 117
MsaG043889 AT5G60480.1 36.318 201 99 5 32 229 30 204 2.79e-30 112
MsaG043889 AT5G42780.1 31.000 200 108 4 38 231 63 238 1.28e-22 92.8
MsaG043889 AT1G75240.1 59.701 67 27 0 169 235 237 303 7.64e-22 92.0
MsaG043889 AT1G75240.1 63.462 52 19 0 37 88 74 125 5.98e-20 87.0
MsaG043889 AT1G74660.2 64.151 53 19 0 37 89 37 89 4.88e-20 82.0
MsaG043889 AT1G74660.1 64.151 53 19 0 37 89 37 89 7.51e-20 81.6
MsaG043889 AT5G39760.1 51.562 64 31 0 171 234 199 262 2.49e-17 80.1
MsaG043889 AT5G39760.1 53.846 52 22 1 39 88 56 107 1.18e-11 63.9
MsaG043889 AT3G28917.1 54.717 53 23 1 37 88 31 83 1.35e-15 70.5
MsaG043889 AT1G18835.1 54.717 53 21 1 37 89 24 73 2.68e-14 66.6

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTGGAAGATTCCGAAAA+AGG 0.318551 8:+52747902 MsaT043889.1:CDS
GGTGGTATTGGTGGTGGTGG+TGG 0.382496 8:+52747780 MsaT043889.1:CDS
TTCTGAAGCAAATAGTTTCC+AGG 0.399589 8:+52747407 MsaT043889.1:CDS
CCACCTCTATCTTTGTATGA+AGG 0.411052 8:+52747693 MsaT043889.1:CDS
CACAATCTAAGAAGAGGTTC+AGG 0.411134 8:+52747826 MsaT043889.1:CDS
CTAAGAAGAGGTTCAGGTCT+AGG 0.417148 8:+52747832 MsaT043889.1:CDS
ATTGGAGGTTATGCCCTTGA+TGG 0.419532 8:+52747468 MsaT043889.1:CDS
CACCTCTATCTTTGTATGAA+GGG 0.435863 8:+52747694 MsaT043889.1:CDS
CCAATACCACCACTATTGAT+AGG 0.443604 8:-52747768 None:intergenic
AAGAACCACGCTTCCAGCAT+TGG 0.445138 8:+52747450 MsaT043889.1:CDS
CTTGCTGCTCCAGTCGTAGA+AGG 0.451779 8:-52747654 None:intergenic
TAGTTTCCAGGTAAATTTCA+AGG 0.451787 8:+52747419 MsaT043889.1:CDS
AGTGGTGGTATTGGTGGTGG+TGG 0.465382 8:+52747777 MsaT043889.1:CDS
CATGGAGCACTAAACGAGAT+AGG 0.482608 8:-52747612 None:intergenic
CATCACTTCGTTGGCTTCAG+TGG 0.483839 8:-52747331 None:intergenic
ATCAATAGTGGTGGTATTGG+TGG 0.485331 8:+52747771 MsaT043889.1:CDS
ACCTCTATCTTTGTATGAAG+GGG 0.485817 8:+52747695 MsaT043889.1:CDS
CCTATCAATAGTGGTGGTAT+TGG 0.499305 8:+52747768 MsaT043889.1:CDS
AACATTCCTTGAAATTTACC+TGG 0.503849 8:-52747425 None:intergenic
AAGGGCATAACCTCCAATGC+TGG 0.508828 8:-52747463 None:intergenic
AATAGTGGTGGTATTGGTGG+TGG 0.510513 8:+52747774 MsaT043889.1:CDS
CATAATAACAAGCATACCCT+TGG 0.523934 8:+52747993 MsaT043889.1:CDS
TAGAAGGAGTCACTGCTTGA+GGG 0.527054 8:-52747638 None:intergenic
AAAACTCACAACAACCATCA+AGG 0.527865 8:-52747482 None:intergenic
TGTGTTACTCATCACTTCGT+TGG 0.534717 8:-52747340 None:intergenic
TTCTACGACTGGAGCAGCAA+GGG 0.539720 8:+52747656 MsaT043889.1:CDS
GTCACTGCTTGAGGGAGGCA+TGG 0.548178 8:-52747630 None:intergenic
GTAGAAGGAGTCACTGCTTG+AGG 0.555326 8:-52747639 None:intergenic
GCAGTGACTCCTTCTACGAC+TGG 0.571024 8:+52747645 MsaT043889.1:CDS
AATGTACCTTCATCTCCAGC+AGG 0.574198 8:-52747507 None:intergenic
AACCACGCTTCCAGCATTGG+AGG 0.577709 8:+52747453 MsaT043889.1:CDS
CTTCTACGACTGGAGCAGCA+AGG 0.595181 8:+52747655 MsaT043889.1:CDS
CAACGACAGTTTCCTTCCTG+TGG 0.597037 8:-52747572 None:intergenic
AAAGGATGAGAATGTTGTCA+AGG 0.599967 8:+52747920 MsaT043889.1:CDS
AACCTCCAATGCTGGAAGCG+TGG 0.601518 8:-52747455 None:intergenic
GGTCATCACAATCTAAGAAG+AGG 0.614611 8:+52747820 MsaT043889.1:CDS
AAACTCACAACAACCATCAA+GGG 0.621682 8:-52747481 None:intergenic
TCTACGACTGGAGCAGCAAG+GGG 0.642048 8:+52747657 MsaT043889.1:CDS
CAGCAAAGAAGAGATGGTGA+TGG 0.647827 8:+52747308 None:intergenic
CCTTCATACAAAGATAGAGG+TGG 0.648904 8:-52747693 None:intergenic
CCTCTATCTTTGTATGAAGG+GGG 0.651375 8:+52747696 MsaT043889.1:CDS
GTTGTTGAGCCTATCAATAG+TGG 0.655020 8:+52747759 MsaT043889.1:CDS
AAGGAGTCACTGCTTGAGGG+AGG 0.663240 8:-52747635 None:intergenic
GTTGAGCCTATCAATAGTGG+TGG 0.692444 8:+52747762 MsaT043889.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 52747321 52748028 52747321 ID=MsaG043889
Chr8 mRNA 52747321 52748028 52747321 ID=MsaT043889.1;Parent=MsaG043889
Chr8 exon 52747321 52748028 52747321 ID=MsaT043889.1.exon1;Parent=MsaT043889.1
Chr8 CDS 52747321 52748028 52747321 ID=cds.MsaT043889.1;Parent=MsaT043889.1
Gene Sequence

>MsaT043889.1

ATGGTGATGGCCACTGAAGCCAACGAAGTGATGAGTAACACATCACAAGAGAAAATATCAACTCGAGTAGAAGAATCTCATATGAATTCTGAAGCAAATAGTTTCCAGGTAAATTTCAAGGAATGTTTGAAGAACCACGCTTCCAGCATTGGAGGTTATGCCCTTGATGGTTGTTGTGAGTTTTTACCTGCTGGAGATGAAGGTACATTAGATTTTTTTAAATGTGGTGCTTGTGATTGTCATAAAAACTTCCACAGGAAGGAAACTGTCGTTGTTTCTAGAGATATGACTCCTATCTCGTTTAGTGCTCCATGCCTCCCTCAAGCAGTGACTCCTTCTACGACTGGAGCAGCAAGGGGCACAATCAATTCTCCACCTCTATCTTTGTATGAAGGGGGCTATATTTTGAGGGAGGATCTTGAACAAAATGTTCATCATGTTGTTGAGCCTATCAATAGTGGTGGTATTGGTGGTGGTGGTGGTGGTGGTGGTGGAGAAGGGTCATCACAATCTAAGAAGAGGTTCAGGTCTAGGTTCACACATGAACAAAGAGAAAAGATGCTTGATTTTGCTGGGGCACGAGGTTGGAAGATTCCGAAAAAGGATGAGAATGTTGTCAAGGAGTTTTGTAACGAGATTGGGGTGAAGCTTCAAGTTTTTAAGGTTTGGGTTCATAATAACAAGCATACCCTTGGTAAAAAGCCCTAA
Protein sequence

>MsaT043889.1

MVMATEANEVMSNTSQEKISTRVEESHMNSEANSFQVNFKECLKNHASSIGGYALDGCCEFLPAGDEGTLDFFKCGACDCHKNFHRKETVVVSRDMTPISFSAPCLPQAVTPSTTGAARGTINSPPLSLYEGGYILREDLEQNVHHVVEPINSGGIGGGGGGGGGEGSSQSKKRFRSRFTHEQREKMLDFAGARGWKIPKKDENVVKEFCNEIGVKLQVFKVWVHNNKHTLGKKP*