AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043890 MtrunA17_Chr8g0367931 99.010 505 5 0 103 607 70 574 0.0 1005
MsaG043890 MtrunA17_Chr2g0315941 70.833 48 14 0 57 104 49 96 3.60e-13 70.1
MsaG043890 MtrunA17_Chr8g0367691 69.388 49 15 0 56 104 49 97 3.81e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043890 AT3G26570.2 78.641 515 100 4 103 607 73 587 0.0 773
MsaG043890 AT3G26570.1 78.641 515 100 4 103 607 99 613 0.0 772

Find 135 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTTTGGTCTAGTTTA+TGG 0.115139 8:+52773003 MsaT043890.1:CDS
GCTGCAGCTGCTGCTTGTCC+TGG 0.170623 8:-52773464 None:intergenic
GAGGAGCTGGAGCTGTATTT+TGG 0.186713 8:+52773025 MsaT043890.1:CDS
GAAACTAGGGCTACCGATAT+CGG 0.248191 8:+52774051 MsaT043890.1:CDS
CCATTGTTTATGACATCTTT+AGG 0.258339 8:+52771340 MsaT043890.1:CDS
GAAGAATCTCATACAAATTC+TGG 0.264006 8:+52762213 MsaT043890.1:CDS
TGGAACCGTCGGGGCTTTCC+TGG 0.279990 8:+52773586 MsaT043890.1:CDS
TCCGGTTGGTGCTACCTTAT+CGG 0.280482 8:+52774183 MsaT043890.1:CDS
TGCTGCTGGTACTTGGTTAC+AGG 0.284265 8:+52771654 MsaT043890.1:CDS
GTTCTTGCTTGGGGTGGATT+TGG 0.288654 8:+52773899 MsaT043890.1:CDS
AGCTTCTTCTCTGATATTAC+AGG 0.298759 8:+52773692 MsaT043890.1:CDS
GGTGGATTTGGAATCGTTGC+AGG 0.303853 8:+52773911 MsaT043890.1:CDS
CAGAGAAGAAGCTTATGTTT+TGG 0.304120 8:-52773682 None:intergenic
CACTTGCTGCTGCTGGTACT+TGG 0.307974 8:+52771647 MsaT043890.1:CDS
TTCATGGGCGGTTACTATTC+CGG 0.308155 8:+52774165 MsaT043890.1:CDS
CTGCAGCTGCTGCTTGTCCT+GGG 0.310215 8:-52773463 None:intergenic
GTTCTGTTTGCTTCGAAACT+AGG 0.311873 8:+52774037 MsaT043890.1:CDS
GAAGATGTCCGAGTTGTTTA+CGG 0.312285 8:-52773622 None:intergenic
ATGTGGCCCCAAGGACTAAA+AGG 0.326031 8:+52760011 MsaT043890.1:CDS
TCCGATTGATGTTCTTGCTT+GGG 0.350128 8:+52773889 MsaT043890.1:CDS
CAAGGACTAAAAGGAAATTC+TGG 0.351933 8:+52760020 MsaT043890.1:CDS
GAGAATAACGATGAAGTATC+AGG 0.354438 8:+52771238 MsaT043890.1:CDS
GCTGTATTTCTCGGCGTAAC+TGG 0.359205 8:+52773497 MsaT043890.1:CDS
TGAAGGAGATTTGTGCTTCA+TGG 0.362113 8:+52774149 MsaT043890.1:CDS
CAAGTCAAATACTCCACATT+TGG 0.370050 8:+52773649 MsaT043890.1:CDS
CTTTCTTCACTTGCTGCTGC+TGG 0.370685 8:+52771640 MsaT043890.1:CDS
ACGCAGTTTGCATCATACTA+TGG 0.372671 8:+52772940 MsaT043890.1:intron
AAAACTCGCAACAACCATCT+AGG 0.381882 8:-52762305 None:intergenic
ACTCAACTTGAAATAGTTTA+CGG 0.382692 8:+52773725 MsaT043890.1:CDS
TTCCGATTGATGTTCTTGCT+TGG 0.384604 8:+52773888 MsaT043890.1:CDS
GCACCAATTGCTGTATTTCT+CGG 0.392067 8:+52773488 MsaT043890.1:CDS
TACAGAGTTATAGCTACAAT+TGG 0.393371 8:+52773950 MsaT043890.1:CDS
AATGCCTGGCTTAGAGCTAT+TGG 0.394818 8:-52773557 None:intergenic
AGTCCTTGGGGCCACATTGA+GGG 0.402049 8:-52760005 None:intergenic
ACACTAGTTGGTGCAGTAAT+GGG 0.403375 8:+52774082 MsaT043890.1:CDS
GACATCTTTAGGACAAGGTT+TGG 0.406475 8:+52771351 MsaT043890.1:CDS
ATTGGAGGTTATGCCCTAGA+TGG 0.412345 8:+52762291 MsaT043890.1:CDS
CATTAGCTTGTGGAACCGTC+GGG 0.412945 8:+52773576 MsaT043890.1:CDS
AGCCGAGTTTGCGGCTGCCT+CGG 0.415177 8:+52774012 MsaT043890.1:CDS
AAACTAGGGCTACCGATATC+GGG 0.416528 8:+52774052 MsaT043890.1:CDS
GGTCTAGTTTATGGAGGAGC+TGG 0.421494 8:+52773012 MsaT043890.1:CDS
AAGAACCATGCTTCCAGCAT+TGG 0.423367 8:+52762273 MsaT043890.1:CDS
ATTTCAAGTTGAGTACCCTT+TGG 0.427540 8:-52773716 None:intergenic
AATGATGTCTCCAACGCAAT+AGG 0.433068 8:+52773806 MsaT043890.1:CDS
ATGGGAACTTCTGTTGGATC+TGG 0.444826 8:+52771463 MsaT043890.1:CDS
TAGTTTCAGTGTAAAATTCA+AGG 0.449365 8:+52762242 MsaT043890.1:CDS
ATACAATGTGTAGTAGATAC+TGG 0.453343 8:-52772967 None:intergenic
ATCTACTACACATTGTATAG+TGG 0.454887 8:+52772972 MsaT043890.1:CDS
AAGACAAAGATGTTATCATA+TGG 0.454924 8:+52771379 MsaT043890.1:CDS
GCATTAGCTTGTGGAACCGT+CGG 0.458457 8:+52773575 MsaT043890.1:CDS
CACCGAGGCAGCCGCAAACT+CGG 0.458951 8:-52774014 None:intergenic
ACGCCGAGAAATACAGCAAT+TGG 0.460216 8:-52773491 None:intergenic
ACCGATAAGGTAGCACCAAC+CGG 0.463631 8:-52774184 None:intergenic
TAGTCCTTGGGGCCACATTG+AGG 0.472862 8:-52760006 None:intergenic
AGGATTTGCAGCCGAGTTTG+CGG 0.475291 8:+52774003 MsaT043890.1:CDS
TACACTAGTTGGTGCAGTAA+TGG 0.476930 8:+52774081 MsaT043890.1:CDS
GAAGGAGATTTGTGCTTCAT+GGG 0.479920 8:+52774150 MsaT043890.1:CDS
GTGGGAGCAATGGTAGGGTT+TGG 0.480615 8:+52772991 MsaT043890.1:CDS
ATTCTACAAGGCGCAGCAAA+AGG 0.482474 8:+52773851 MsaT043890.1:CDS
GATACTGCTGCTCCCAATAG+TGG 0.485743 8:-52773087 None:intergenic
TAGCAAGAGTGATTTCTTCG+TGG 0.487529 8:+52773055 MsaT043890.1:CDS
GATCTGGTGCATTGTCACTT+AGG 0.491977 8:+52771479 MsaT043890.1:CDS
TTCTGTTTGCTTCGAAACTA+GGG 0.492039 8:+52774038 MsaT043890.1:CDS
CCCCAAGCAAGAACATCAAT+CGG 0.492615 8:-52773890 None:intergenic
CAACATGCTGCAACAGAAAA+CGG 0.492938 8:+52771214 MsaT043890.1:CDS
TCGGCTGCAAATCCTCTTGT+CGG 0.500344 8:-52773995 None:intergenic
TGTATAGTGGGAGCAATGGT+AGG 0.500362 8:+52772985 MsaT043890.1:CDS
GTTGGTGCAGTAATGGGAGT+TGG 0.502537 8:+52774088 MsaT043890.1:CDS
CTCTGATATTACAGGTCCAA+AGG 0.503091 8:+52773700 MsaT043890.1:CDS
TAGGGCATAACCTCCAATGC+TGG 0.506656 8:-52762286 None:intergenic
CTCCAACGCAATAGGTCCAT+TGG 0.509756 8:+52773814 MsaT043890.1:CDS
AGTGTATGTGTCCCCGATAT+CGG 0.513359 8:-52774064 None:intergenic
TCAAGCGTTTACATGTTAAG+TGG 0.513417 8:+52762090 MsaT043890.1:CDS
TACGGATAGTTCTGTTGACC+AGG 0.515812 8:-52773604 None:intergenic
TGGGCGGTTACTATTCCGGT+TGG 0.521858 8:+52774169 MsaT043890.1:CDS
CGTTGCAGGGCTAATGATGT+GGG 0.524372 8:+52773925 MsaT043890.1:CDS
CGGTTCCACAAGCTAATGCC+TGG 0.524860 8:-52773571 None:intergenic
AACCATGCTTCCAGCATTGG+AGG 0.529576 8:+52762276 MsaT043890.1:CDS
AATGCAATGGGAACTTCTGT+TGG 0.531915 8:+52771457 MsaT043890.1:CDS
CCACGTGTAAATCACCGATA+AGG 0.535689 8:-52774197 None:intergenic
TAATGACAGCGAAGAGGAGA+TGG 0.538056 8:+52762125 MsaT043890.1:CDS
CTAAGCCAGGCATTAGCTTG+TGG 0.542312 8:+52773566 MsaT043890.1:CDS
AATGATGTTGCAAATGCAAT+GGG 0.542803 8:+52771445 MsaT043890.1:CDS
CAAGCGTTTACATGTTAAGT+GGG 0.547804 8:+52762091 MsaT043890.1:CDS
AACCTCCAATGCTGGAAGCA+TGG 0.548110 8:-52762278 None:intergenic
CCTAAAGATGTCATAAACAA+TGG 0.548336 8:-52771340 None:intergenic
CCGATTGATGTTCTTGCTTG+GGG 0.552108 8:+52773890 MsaT043890.1:CDS
GTGGATTTGGAATCGTTGCA+GGG 0.552937 8:+52773912 MsaT043890.1:CDS
TTGTCTACAAATGCATCCGA+AGG 0.553473 8:+52773115 MsaT043890.1:CDS
TCGGGGACACATACACTAGT+TGG 0.555114 8:+52774070 MsaT043890.1:CDS
CGAGTTTGCGGCTGCCTCGG+TGG 0.555593 8:+52774015 MsaT043890.1:CDS
ATTGATGTTCTTGCTTGGGG+TGG 0.555893 8:+52773893 MsaT043890.1:CDS
GGGTTTGGTCTAGTTTATGG+AGG 0.557081 8:+52773006 MsaT043890.1:CDS
TCGTTGCAGGGCTAATGATG+TGG 0.557121 8:+52773924 MsaT043890.1:CDS
TGTTTCATGTCATTTGCTCA+CGG 0.557308 8:+52773779 MsaT043890.1:CDS
AGTTTGCATCATACTATGGT+TGG 0.559979 8:+52772944 MsaT043890.1:intron
TCTGATATTACAGGTCCAAA+GGG 0.566683 8:+52773701 MsaT043890.1:CDS
GGCCAATGGACCTATTGCGT+TGG 0.567487 8:-52773816 None:intergenic
ATAGCTAATGCACCGGCCAA+TGG 0.568991 8:-52773830 None:intergenic
TGTGACTAGTACAATGCAGA+AGG 0.570955 8:+52771561 MsaT043890.1:CDS
TACAATTGGAAAGAAAATAA+CGG 0.573343 8:+52773964 MsaT043890.1:CDS
TCTACTACACATTGTATAGT+GGG 0.579166 8:+52772973 MsaT043890.1:CDS
TGTGTTGACAGCTGCAGTGT+TGG 0.582448 8:+52771507 MsaT043890.1:CDS
GTATAGTGGGAGCAATGGTA+GGG 0.585259 8:+52772986 MsaT043890.1:CDS
TAACGATGAAGTATCAGGAA+TGG 0.585273 8:+52771243 MsaT043890.1:CDS
CCTTATCGGTGATTTACACG+TGG 0.586036 8:+52774197 MsaT043890.1:CDS
ACGGTACCTTCATCTCCAGC+AGG 0.587411 8:-52762330 None:intergenic
AAACTCGCAACAACCATCTA+GGG 0.589045 8:-52762304 None:intergenic
GCTAAGTGGGAAAGCAGCAA+AGG 0.591804 8:-52773525 None:intergenic
TAATGATGTTGCAAATGCAA+TGG 0.596839 8:+52771444 MsaT043890.1:CDS
ACATTGTATAGTGGGAGCAA+TGG 0.597997 8:+52772981 MsaT043890.1:CDS
TTGTAGAATAGCTAATGCAC+CGG 0.598942 8:-52773837 None:intergenic
AACGCAATAGGTCCATTGGC+CGG 0.610227 8:+52773818 MsaT043890.1:CDS
CAGCGAAGAGGAGATGGTGA+TGG 0.612857 8:+52762131 MsaT043890.1:CDS
GGTGCATTAGCTATTCTACA+AGG 0.615570 8:+52773839 MsaT043890.1:CDS
AGTGATGATTAACTCATCAC+AGG 0.617217 8:+52762170 MsaT043890.1:CDS
AGAACTATCCGTAAACAACT+CGG 0.621558 8:+52773614 MsaT043890.1:CDS
AAACCCTCAATGTGGCCCCA+AGG 0.625185 8:+52760002 MsaT043890.1:CDS
ATGGGAGTTGGATTCGCGAG+AGG 0.625208 8:+52774100 MsaT043890.1:CDS
TTTATGACATCTTTAGGACA+AGG 0.625714 8:+52771346 MsaT043890.1:CDS
TGTAACAATATGTATAGTAA+TGG 0.630983 8:+52771303 MsaT043890.1:CDS
GCGAAGTAATGACAGCGAAG+AGG 0.631353 8:+52762119 MsaT043890.1:CDS
ACGGAACTTACACCGACAAG+AGG 0.634990 8:+52773983 MsaT043890.1:CDS
GGAGATTTGTGCTTCATGGG+CGG 0.643143 8:+52774153 MsaT043890.1:CDS
GTTGACCAGGAAAGCCCCGA+CGG 0.645332 8:-52773591 None:intergenic
TTCATGTCATTTGCTCACGG+TGG 0.649798 8:+52773782 MsaT043890.1:CDS
ATTAGCTTGTGGAACCGTCG+GGG 0.654249 8:+52773577 MsaT043890.1:CDS
AACTAGGGCTACCGATATCG+GGG 0.656003 8:+52774053 MsaT043890.1:CDS
TGTCTACAAATGCATCCGAA+GGG 0.657192 8:+52773116 MsaT043890.1:CDS
TGTTAGATCAGAAACAGTGA+AGG 0.658218 8:+52774132 MsaT043890.1:CDS
GTGACTAGTACAATGCAGAA+GGG 0.662462 8:+52771562 MsaT043890.1:CDS
GTATACAGTGCAAGTAACCC+AGG 0.669512 8:+52773446 MsaT043890.1:CDS
GTTGCAGGGCTAATGATGTG+GGG 0.715224 8:+52773926 MsaT043890.1:CDS
TGACATGCAAACCCTCAATG+TGG 0.722271 8:+52759994 MsaT043890.1:CDS
CGAAGCAAACAGAACCACCG+AGG 0.756636 8:-52774029 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 52759993 52774252 52759993 ID=MsaG043890
Chr8 mRNA 52759993 52774252 52759993 ID=MsaT043890.1;Parent=MsaG043890
Chr8 exon 52759993 52760041 52759993 ID=MsaT043890.1.exon1;Parent=MsaT043890.1
Chr8 CDS 52759993 52760041 52759993 ID=cds.MsaT043890.1;Parent=MsaT043890.1
Chr8 exon 52762082 52762345 52762082 ID=MsaT043890.1.exon2;Parent=MsaT043890.1
Chr8 CDS 52762082 52762345 52762082 ID=cds.MsaT043890.1;Parent=MsaT043890.1
Chr8 exon 52771167 52771675 52771167 ID=MsaT043890.1.exon3;Parent=MsaT043890.1
Chr8 CDS 52771167 52771675 52771167 ID=cds.MsaT043890.1;Parent=MsaT043890.1
Chr8 exon 52772946 52773137 52772946 ID=MsaT043890.1.exon4;Parent=MsaT043890.1
Chr8 CDS 52772946 52773137 52772946 ID=cds.MsaT043890.1;Parent=MsaT043890.1
Chr8 exon 52773443 52774252 52773443 ID=MsaT043890.1.exon5;Parent=MsaT043890.1
Chr8 CDS 52773443 52774252 52773443 ID=cds.MsaT043890.1;Parent=MsaT043890.1
Gene Sequence

>MsaT043890.1

ATGACATGCAAACCCTCAATGTGGCCCCAAGGACTAAAAGGAAATTCTGTTTTCTTTTCAAGCGTTTACATGTTAAGTGGGCAAGTGCGAAGTAATGACAGCGAAGAGGAGATGGTGATGGCTACGAACGTCAACGAAGTGATGATTAACTCATCACAGGAGAAAATATCAACTCAAGTTGAAGAATCTCATACAAATTCTGGAAGAAATAGTTTCAGTGTAAAATTCAAGGAATGTTTAAAGAACCATGCTTCCAGCATTGGAGGTTATGCCCTAGATGGTTGTTGCGAGTTTTTACCTGCTGGAGATGAAGAAGGAGAAGAGGGTTCAAAAGAAGAAATTCAGCACAATGAACATCACCAACATGCTGCAACAGAAAACGGAGAGAATAACGATGAAGTATCAGGAATGGCTAAAGCATTTCACATATCATCAAGAACAGCTTCTGCTGTAACAATATGTATAGTAATGGCTGCACTTGTTTTTCCATTGTTTATGACATCTTTAGGACAAGGTTTGGCATTGAAGACAAAGATGTTATCATATGGAACACTTTTGTTTGGATTTTACATGGCTTGGAACATTGGTGCTAATGATGTTGCAAATGCAATGGGAACTTCTGTTGGATCTGGTGCATTGTCACTTAGGCAAGCTGTGTTGACAGCTGCAGTGTTGGAGTTTTCAGGGGCATTGTTGATGGGAACTCATGTGACTAGTACAATGCAGAAGGGAATTCTTGTTGCTAATGTTTTTCAGGGGAAGGATACTTTGCTTTTTGCAGGGTTGCTTTCTTCACTTGCTGCTGCTGGTACTTGGTTACAGTTTGCATCATACTATGGTTGGCCAGTATCTACTACACATTGTATAGTGGGAGCAATGGTAGGGTTTGGTCTAGTTTATGGAGGAGCTGGAGCTGTATTTTGGGGTTCACTAGCAAGAGTGATTTCTTCGTGGATTTTTTCGCCACTATTGGGAGCAGCAGTATCGTTTATTGTCTACAAATGCATCCGAAGGTTTGTATACAGTGCAAGTAACCCAGGACAAGCAGCAGCTGCAGCAGCACCAATTGCTGTATTTCTCGGCGTAACTGGAATTTCCTTTGCTGCTTTCCCACTTAGCAAGATTTTTCCAATAGCTCTAAGCCAGGCATTAGCTTGTGGAACCGTCGGGGCTTTCCTGGTCAACAGAACTATCCGTAAACAACTCGGACATCTTCTAGACAAGTCAAATACTCCACATTTGGAACCCGTCCCCCAAAACATAAGCTTCTTCTCTGATATTACAGGTCCAAAGGGTACTCAACTTGAAATAGTTTACGGAGTTTTCGGTTATATGCAGGTTTTATCAGCGTGTTTCATGTCATTTGCTCACGGTGGAAATGATGTCTCCAACGCAATAGGTCCATTGGCCGGTGCATTAGCTATTCTACAAGGCGCAGCAAAAGGAGCTGATATAGTTATTCCGATTGATGTTCTTGCTTGGGGTGGATTTGGAATCGTTGCAGGGCTAATGATGTGGGGTTACAGAGTTATAGCTACAATTGGAAAGAAAATAACGGAACTTACACCGACAAGAGGATTTGCAGCCGAGTTTGCGGCTGCCTCGGTGGTTCTGTTTGCTTCGAAACTAGGGCTACCGATATCGGGGACACATACACTAGTTGGTGCAGTAATGGGAGTTGGATTCGCGAGAGGATTTAACAATGTTAGATCAGAAACAGTGAAGGAGATTTGTGCTTCATGGGCGGTTACTATTCCGGTTGGTGCTACCTTATCGGTGATTTACACGTGGATTTTGACTAAGCTTTTGTCTTATGTTTTATGA

Protein sequence

>MsaT043890.1

MTCKPSMWPQGLKGNSVFFSSVYMLSGQVRSNDSEEEMVMATNVNEVMINSSQEKISTQVEESHTNSGRNSFSVKFKECLKNHASSIGGYALDGCCEFLPAGDEEGEEGSKEEIQHNEHHQHAATENGENNDEVSGMAKAFHISSRTASAVTICIVMAALVFPLFMTSLGQGLALKTKMLSYGTLLFGFYMAWNIGANDVANAMGTSVGSGALSLRQAVLTAAVLEFSGALLMGTHVTSTMQKGILVANVFQGKDTLLFAGLLSSLAAAGTWLQFASYYGWPVSTTHCIVGAMVGFGLVYGGAGAVFWGSLARVISSWIFSPLLGAAVSFIVYKCIRRFVYSASNPGQAAAAAAPIAVFLGVTGISFAAFPLSKIFPIALSQALACGTVGAFLVNRTIRKQLGHLLDKSNTPHLEPVPQNISFFSDITGPKGTQLEIVYGVFGYMQVLSACFMSFAHGGNDVSNAIGPLAGALAILQGAAKGADIVIPIDVLAWGGFGIVAGLMMWGYRVIATIGKKITELTPTRGFAAEFAAASVVLFASKLGLPISGTHTLVGAVMGVGFARGFNNVRSETVKEICASWAVTIPVGATLSVIYTWILTKLLSYVL*