AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043914

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043914 MtrunA17_Chr2g0333541 77.551 294 41 3 1 287 1 276 4.56e-151 423
MsaG043914 MtrunA17_Chr8g0368371 92.908 141 10 0 1 141 1 141 2.10e-93 272
MsaG043914 MtrunA17_Chr2g0333621 37.748 302 155 9 1 279 20 311 1.28e-45 156
MsaG043914 MtrunA17_Chr8g0336681 41.532 248 118 8 57 279 90 335 8.64e-45 155
MsaG043914 MtrunA17_Chr2g0333641 37.415 294 149 11 6 277 25 305 1.84e-42 148
MsaG043914 MtrunA17_Chr4g0002801 36.164 318 156 10 6 279 16 330 2.68e-42 148
MsaG043914 MtrunA17_Chr5g0400621 44.330 194 94 5 97 282 144 331 4.52e-39 139
MsaG043914 MtrunA17_Chr4g0002781 33.939 330 157 10 6 279 25 349 7.95e-38 136
MsaG043914 MtrunA17_Chr2g0333611 44.149 188 83 6 86 265 21 194 9.41e-37 130
MsaG043914 MtrunA17_Chr4g0054581 40.206 194 105 3 95 279 183 374 2.81e-36 133
MsaG043914 MtrunA17_Chr2g0280161 42.391 184 90 5 103 275 168 346 3.93e-36 132
MsaG043914 MtrunA17_Chr5g0400661 41.294 201 93 6 101 279 137 334 6.35e-36 131
MsaG043914 MtrunA17_Chr2g0307131 38.342 193 107 4 95 279 163 351 2.78e-35 130
MsaG043914 MtrunA17_Chr7g0249811 34.161 322 139 11 11 279 27 328 1.62e-34 127
MsaG043914 MtrunA17_Chr4g0033291 37.308 260 131 12 40 281 80 325 3.46e-34 126
MsaG043914 MtrunA17_Chr2g0307141 39.773 176 95 3 112 279 183 355 1.60e-33 125
MsaG043914 MtrunA17_Chr7g0225901 37.640 178 107 2 90 266 66 240 5.13e-33 121
MsaG043914 MtrunA17_Chr4g0054551 34.231 260 132 9 47 277 74 323 8.88e-33 122
MsaG043914 MtrunA17_Chr4g0033391 37.949 195 104 6 95 281 132 317 1.03e-32 122
MsaG043914 MtrunA17_Chr4g0054561 33.077 260 135 9 47 277 74 323 2.06e-32 122
MsaG043914 MtrunA17_Chr5g0400671 32.530 249 132 8 58 281 88 325 2.99e-31 119
MsaG043914 MtrunA17_Chr5g0400641 40.127 157 90 2 115 270 1 154 7.41e-31 113
MsaG043914 MtrunA17_Chr4g0033281 34.894 235 122 8 57 275 98 317 2.88e-30 116
MsaG043914 MtrunA17_Chr4g0049331 36.683 199 111 5 91 277 110 305 4.53e-30 115
MsaG043914 MtrunA17_Chr6g0468491 37.755 196 102 5 96 279 122 309 3.15e-29 113
MsaG043914 MtrunA17_Chr4g0054621 32.093 215 123 6 78 279 133 337 2.39e-24 100
MsaG043914 MtrunA17_Chr6g0468461 36.257 171 90 4 97 266 159 311 5.11e-23 97.1
MsaG043914 MtrunA17_Chr4g0054571 37.073 205 107 8 93 281 132 330 6.55e-23 96.3
MsaG043914 MtrunA17_Chr7g0233441 34.731 167 83 6 117 275 44 192 1.02e-19 85.1
MsaG043914 MtrunA17_Chr4g0002831 35.028 177 91 7 114 281 45 206 2.45e-17 78.6
MsaG043914 MtrunA17_Chr4g0002851 33.333 168 82 5 116 275 47 192 2.70e-17 78.2
MsaG043914 MtrunA17_Chr8g0365711 26.230 244 149 9 64 281 179 417 2.51e-14 72.8
MsaG043914 MtrunA17_Chr2g0298751 26.131 199 129 6 83 266 21 216 4.08e-12 64.7
MsaG043914 MtrunA17_Chr8g0346161 23.899 159 105 6 102 250 280 432 4.06e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043914 AT4G37850.2 38.298 188 106 3 97 275 135 321 6.34e-34 126
MsaG043914 AT4G37850.1 38.298 188 106 3 97 275 135 321 6.34e-34 126
MsaG043914 AT2G22750.2 43.182 176 98 1 105 278 119 294 2.60e-33 124
MsaG043914 AT2G22750.5 43.182 176 98 1 105 278 143 318 3.73e-33 124
MsaG043914 AT2G22750.3 43.429 175 97 1 105 277 147 321 4.16e-33 124
MsaG043914 AT2G22750.1 43.820 178 92 2 105 274 119 296 4.58e-33 123
MsaG043914 AT2G22750.4 43.820 178 92 2 105 274 147 324 6.39e-33 123
MsaG043914 AT2G22760.1 35.149 202 104 8 94 280 106 295 6.07e-28 109
MsaG043914 AT2G22760.2 34.807 181 100 6 94 267 106 275 3.82e-26 104
MsaG043914 AT2G22770.1 33.889 180 106 3 112 278 132 311 6.32e-25 102
MsaG043914 AT4G29930.2 26.000 200 127 5 73 266 13 197 4.74e-15 73.2
MsaG043914 AT4G29930.6 25.381 197 135 4 73 266 13 200 5.77e-15 72.8
MsaG043914 AT5G57150.5 26.667 195 132 6 73 258 14 206 1.73e-14 71.6
MsaG043914 AT4G29930.5 25.628 199 127 5 73 265 13 196 2.38e-14 71.2
MsaG043914 AT5G57150.1 27.094 203 137 7 73 266 14 214 2.59e-14 71.6
MsaG043914 AT4G29930.1 24.424 217 138 5 73 266 13 226 1.03e-13 69.7
MsaG043914 AT4G09820.1 32.680 153 76 4 100 251 351 477 1.59e-13 70.9
MsaG043914 AT5G41315.3 31.098 164 87 5 104 244 438 598 1.69e-13 70.9
MsaG043914 AT5G41315.1 31.098 164 87 5 104 244 433 593 1.80e-13 70.9
MsaG043914 AT5G41315.2 31.098 164 87 5 104 244 441 601 2.02e-13 70.9
MsaG043914 AT5G57150.3 27.568 185 123 7 83 258 25 207 2.38e-13 68.2
MsaG043914 AT5G57150.2 27.568 185 123 7 83 258 25 207 2.97e-13 68.2
MsaG043914 AT4G29930.3 24.074 216 138 5 73 265 13 225 6.21e-13 67.8
MsaG043914 AT4G29930.7 24.074 216 138 5 73 265 13 225 6.21e-13 67.8
MsaG043914 AT5G57150.4 26.486 185 125 6 83 258 25 207 8.15e-13 67.4
MsaG043914 AT1G63650.3 29.299 157 94 2 104 244 397 552 1.42e-12 68.2
MsaG043914 AT1G63650.1 29.299 157 94 2 104 244 397 552 1.42e-12 68.2
MsaG043914 AT1G63650.2 29.299 157 94 2 104 244 397 552 1.42e-12 68.2
MsaG043914 AT4G17880.1 23.684 190 123 5 74 247 365 548 2.79e-11 64.3

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAGTGTCTTTGTCAATT+AGG 0.241012 8:-53194567 None:intergenic
TGTCTTTGTCAATTAGGATA+TGG 0.244498 8:-53194561 None:intergenic
CAAGACATTGGTAGTTGTTA+TGG 0.303801 8:-53194749 None:intergenic
GAGGTTGAAGCTAGGGTTCT+AGG 0.308353 8:+53194640 MsaT043914.1:CDS
ACTACCAATGTCTTGCCATT+TGG 0.326554 8:+53194757 MsaT043914.1:CDS
CTTCTTTAATCCAGGTATAA+AGG 0.327011 8:-53191371 None:intergenic
AAGAAAGATCATAGAACTTT+CGG 0.343414 8:+53191348 MsaT043914.1:CDS
ACTACTTGCCTGAGCAATAA+TGG 0.366205 8:-53194797 None:intergenic
ACTAGGCCAGTTGTGGCAAT+AGG 0.371153 8:-53191067 None:intergenic
ACATAATGTCAGAGAGGAAT+AGG 0.386492 8:+53191311 MsaT043914.1:CDS
AATGGCTTTGTAGATTGATA+AGG 0.402893 8:+53194423 MsaT043914.1:intron
AGTGTCTCAGAAGCGCTTCT+AGG 0.408035 8:-53191283 None:intergenic
TAGGATATGGGATCTTGTTA+TGG 0.409337 8:-53194548 None:intergenic
GTCTTTGTCAATTAGGATAT+GGG 0.412094 8:-53194560 None:intergenic
AGAGACAATATAGAATTTGT+TGG 0.427912 8:-53191163 None:intergenic
GAACTTTCGGCCTTTATACC+TGG 0.434477 8:+53191361 MsaT043914.1:CDS
TCTGATACAGGATATCTCTA+AGG 0.449935 8:-53191021 None:intergenic
TGAAAACTGAAGAATGTTAC+GGG 0.453225 8:+53194599 MsaT043914.1:CDS
ATGAAAACTGAAGAATGTTA+CGG 0.462740 8:+53194598 MsaT043914.1:CDS
TGATATCGCCATTATTGCTC+AGG 0.463699 8:+53194789 MsaT043914.1:CDS
CTAGGCCAGTTGTGGCAATA+GGG 0.464667 8:-53191066 None:intergenic
GATCTTGTGGTGTCTGATAC+AGG 0.476165 8:-53191033 None:intergenic
AATATAGAATTTGTTGGTAT+CGG 0.477242 8:-53191157 None:intergenic
ATCTTCATCAAATGTGTTGT+TGG 0.478390 8:-53190996 None:intergenic
ATTGACAAAGACACTGCAAT+TGG 0.483400 8:+53194571 MsaT043914.1:CDS
AGTGTCCCTATTGCCACAAC+TGG 0.491296 8:+53191061 MsaT043914.1:CDS
AGTTGAATGTGACACCTTGA+TGG 0.493789 8:-53191101 None:intergenic
AGACTCACCTTCTTTAATCC+AGG 0.495262 8:-53191379 None:intergenic
CTTTATACCTGGATTAAAGA+AGG 0.504848 8:+53191372 MsaT043914.1:CDS
CACTTGTAGAGGTTGAAGCT+AGG 0.509415 8:+53194632 MsaT043914.1:CDS
AGAGATGAAGAAGTTGAAGC+TGG 0.509669 8:-53194726 None:intergenic
AACTTGAAGAACGTGTGAAA+GGG 0.515188 8:+53194482 MsaT043914.1:CDS
GCTTCTTCGAATTTGACCCT+TGG 0.520716 8:-53191261 None:intergenic
CAACTTGAAGAACGTGTGAA+AGG 0.521724 8:+53194481 MsaT043914.1:CDS
ACTTGTAGAGGTTGAAGCTA+GGG 0.522502 8:+53194633 MsaT043914.1:CDS
AGGTTGAAGCTAGGGTTCTA+GGG 0.524896 8:+53194641 MsaT043914.1:CDS
TTTGCCTTTGTCGTCATCAA+CGG 0.534333 8:+53191234 MsaT043914.1:CDS
ACGACAAAGGCAAAGAATCA+TGG 0.539001 8:-53191225 None:intergenic
TTCATTGTGGCATGCAAGAG+GGG 0.542951 8:+53194683 MsaT043914.1:CDS
GAATCATGGTGTTGCTCAGT+TGG 0.544880 8:-53191211 None:intergenic
TGTTGGTATCGGTAATGAGT+TGG 0.561174 8:-53191146 None:intergenic
ATATCAAGAGTATTTCCAAA+TGG 0.561322 8:-53194772 None:intergenic
TCGTCATCAACGGCTAGCCA+AGG 0.562738 8:+53191244 MsaT043914.1:CDS
GGTATCGGTAATGAGTTGGC+TGG 0.564211 8:-53191142 None:intergenic
ATTCATTGTGGCATGCAAGA+GGG 0.569505 8:+53194682 MsaT043914.1:CDS
ATTTCCAAATGGCAAGACAT+TGG 0.570505 8:-53194761 None:intergenic
TGATAAGGTTCATGTGGTAA+CGG 0.571985 8:+53194438 MsaT043914.1:CDS
GTAGATTGATAAGGTTCATG+TGG 0.584148 8:+53194432 MsaT043914.1:intron
GAGATGAAGAAGTTGAAGCT+GGG 0.593619 8:-53194725 None:intergenic
GATTCATTGTGGCATGCAAG+AGG 0.605687 8:+53194681 MsaT043914.1:CDS
AGTCAACAACTCATCCATCA+AGG 0.612611 8:+53191087 MsaT043914.1:CDS
GCGAAATGAATCACTTGTAG+AGG 0.614559 8:+53194621 MsaT043914.1:CDS
CGTCATCAACGGCTAGCCAA+GGG 0.623288 8:+53191245 MsaT043914.1:CDS
TGATGGATGAGTTGTTGACT+AGG 0.626332 8:-53191084 None:intergenic
GACATCAAGAAGAAAGACAC+AGG 0.627807 8:+53194514 MsaT043914.1:CDS
GTGTTAATCAAGATTCATTG+TGG 0.641857 8:+53194670 MsaT043914.1:CDS
TCATGGTGTTGCTCAGTTGG+TGG 0.641914 8:-53191208 None:intergenic
TGGCATGCAAGAGGGGATTG+TGG 0.656870 8:+53194690 MsaT043914.1:CDS
CTAGCCGTTGATGACGACAA+AGG 0.683159 8:-53191238 None:intergenic
GGGACACTGAATTGATCTTG+TGG 0.685755 8:-53191046 None:intergenic
GTTGTTGACTAGGCCAGTTG+TGG 0.701059 8:-53191074 None:intergenic
TAGATCACATAATGTCAGAG+AGG 0.710986 8:+53191305 MsaT043914.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 53190974 53195467 53190974 ID=MsaG043914
Chr8 mRNA 53190974 53195467 53190974 ID=MsaT043914.1;Parent=MsaG043914
Chr8 exon 53190974 53191393 53190974 ID=MsaT043914.1.exon1;Parent=MsaT043914.1
Chr8 CDS 53190974 53191393 53190974 ID=cds.MsaT043914.1;Parent=MsaT043914.1
Chr8 exon 53194436 53194814 53194436 ID=MsaT043914.1.exon2;Parent=MsaT043914.1
Chr8 CDS 53194436 53194814 53194436 ID=cds.MsaT043914.1;Parent=MsaT043914.1
Chr8 exon 53195400 53195467 53195400 ID=MsaT043914.1.exon3;Parent=MsaT043914.1
Chr8 CDS 53195400 53195467 53195400 ID=cds.MsaT043914.1;Parent=MsaT043914.1
Gene Sequence

>MsaT043914.1

ATGGAAGAATGTTTGTGTCACACCAACAACACATTTGATGAAGATTTCCTTAGAGATATCCTGTATCAGACACCACAAGATCAATTCAGTGTCCCTATTGCCACAACTGGCCTAGTCAACAACTCATCCATCAAGGTGTCACATTCAACTCAACATGCTGAAGAAAAACCAGCCAACTCATTACCGATACCAACAAATTCTATATTGTCTCTTGACGAATCTGTTACATTGTTACCACCAACTGAGCAACACCATGATTCTTTGCCTTTGTCGTCATCAACGGCTAGCCAAGGGTCAAATTCGAAGAAGCCTAGAAGCGCTTCTGAGACACTAGATCACATAATGTCAGAGAGGAATAGGAGACAGCTACTTACAAGAAAGATCATAGAACTTTCGGCCTTTATACCTGGATTAAAGAAGATTGATAAGGTTCATGTGGTAACGGAAGCTATCAATTATGTGAAACAACTTGAAGAACGTGTGAAAGGGCTAGAAGAAGACATCAAGAAGAAAGACACAGGATCAGTGAGCACCATAACAAGATCCCATATCCTAATTGACAAAGACACTGCAATTGGTGAAATGAAAACTGAAGAATGTTACGGGCGAAATGAATCACTTGTAGAGGTTGAAGCTAGGGTTCTAGGGAATGAAGTGTTAATCAAGATTCATTGTGGCATGCAAGAGGGGATTGTGGTCAATATAATGTCCCAGCTTCAACTTCTTCATCTCTCCATAACAACTACCAATGTCTTGCCATTTGGAAATACTCTTGATATCGCCATTATTGCTCAGGCAATGAAGGATCTAGTGAAAAAACTAAGAGAAGTGGCTATGTTGCAAGCATGTGACGATGTGCAACAATGA
Protein sequence

>MsaT043914.1

MEECLCHTNNTFDEDFLRDILYQTPQDQFSVPIATTGLVNNSSIKVSHSTQHAEEKPANSLPIPTNSILSLDESVTLLPPTEQHHDSLPLSSSTASQGSNSKKPRSASETLDHIMSERNRRQLLTRKIIELSAFIPGLKKIDKVHVVTEAINYVKQLEERVKGLEEDIKKKDTGSVSTITRSHILIDKDTAIGEMKTEECYGRNESLVEVEARVLGNEVLIKIHCGMQEGIVVNIMSQLQLLHLSITTTNVLPFGNTLDIAIIAQAMKDLVKKLREVAMLQACDDVQQ*