AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043943

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043943 MtrunA17_Chr8g0368721 85.350 314 39 3 1 309 1 312 6.61e-113 336
MsaG043943 MtrunA17_Chr6g0470151 48.021 379 151 7 1 355 1 357 4.39e-72 233
MsaG043943 MtrunA17_Chr8g0366921 52.744 328 128 6 1 309 1 320 2.00e-66 216
MsaG043943 MtrunA17_Chr2g0289181 44.635 233 68 2 315 486 1 233 1.49e-57 190
MsaG043943 MtrunA17_Chr7g0266621 51.935 310 140 4 1 309 1 302 1.04e-48 169
MsaG043943 MtrunA17_Chr2g0293901 56.069 173 69 4 1 170 1 169 2.68e-35 130
MsaG043943 MtrunA17_Chr1g0202461 53.535 99 44 2 305 403 239 335 5.80e-29 117
MsaG043943 MtrunA17_Chr8g0348311 46.939 98 49 2 305 402 134 228 2.60e-24 103
MsaG043943 MtrunA17_Chr7g0248691 47.917 96 49 1 307 402 133 227 3.97e-24 102
MsaG043943 MtrunA17_Chr2g0293481 45.133 113 50 3 308 410 238 348 8.70e-22 98.2
MsaG043943 MtrunA17_Chr8g0387501 56.944 72 30 1 329 400 130 200 2.53e-21 93.6
MsaG043943 MtrunA17_Chr4g0053281 51.471 68 33 0 329 396 267 334 1.65e-19 89.7
MsaG043943 MtrunA17_Chr5g0401821 51.471 68 33 0 329 396 200 267 3.23e-19 87.4
MsaG043943 MtrunA17_Chr4g0071521 56.716 67 28 1 324 390 242 307 6.47e-19 87.4
MsaG043943 MtrunA17_Chr2g0319961 56.061 66 29 0 323 388 75 140 1.08e-18 85.9
MsaG043943 MtrunA17_Chr8g0379461 31.013 158 84 3 326 470 74 219 1.15e-18 85.5
MsaG043943 MtrunA17_Chr3g0139021 43.158 95 53 1 300 393 285 379 1.49e-18 87.8
MsaG043943 MtrunA17_Chr1g0184691 57.627 59 25 0 329 387 294 352 5.71e-18 85.5
MsaG043943 MtrunA17_Chr7g0270051 55.000 60 27 0 329 388 54 113 1.00e-17 83.2
MsaG043943 MtrunA17_Chr2g0284321 53.968 63 29 0 326 388 33 95 1.38e-17 81.6
MsaG043943 MtrunA17_Chr7g0228941 54.098 61 28 0 329 389 53 113 1.53e-17 79.0
MsaG043943 MtrunA17_Chr1g0147881 47.826 69 36 0 329 397 263 331 1.78e-17 83.6
MsaG043943 MtrunA17_Chr7g0218081 43.373 83 45 2 328 410 410 490 1.78e-15 79.0
MsaG043943 MtrunA17_Chr7g0218081 49.275 69 33 2 324 392 224 290 1.84e-15 79.0
MsaG043943 MtrunA17_Chr7g0263531 42.574 101 43 4 305 391 324 423 2.30e-15 78.2
MsaG043943 MtrunA17_Chr7g0263531 55.385 65 27 2 324 388 185 247 2.34e-15 78.2
MsaG043943 MtrunA17_Chr7g0266611 56.061 66 29 0 383 448 2 67 3.21e-15 73.6
MsaG043943 MtrunA17_Chr7g0243891 51.220 82 35 3 311 392 202 278 5.29e-15 77.4
MsaG043943 MtrunA17_Chr7g0243891 39.189 74 44 1 328 401 374 446 6.88e-12 67.8
MsaG043943 MtrunA17_Chr3g0079691 49.315 73 35 2 326 398 278 348 6.80e-15 77.4
MsaG043943 MtrunA17_Chr3g0079691 43.478 69 38 1 328 396 495 562 1.30e-13 73.6
MsaG043943 MtrunA17_Chr7g0249311 39.048 105 49 3 328 432 392 481 1.42e-14 76.3
MsaG043943 MtrunA17_Chr7g0249311 43.902 82 44 2 318 399 169 248 5.06e-14 74.7
MsaG043943 MtrunA17_Chr4g0042831 43.750 80 44 1 328 407 278 356 1.42e-14 75.5
MsaG043943 MtrunA17_Chr4g0042831 41.176 68 39 1 321 388 138 204 6.36e-11 63.9
MsaG043943 MtrunA17_Chr5g0418191 46.269 67 35 1 328 394 320 385 1.74e-14 75.5
MsaG043943 MtrunA17_Chr5g0418191 35.971 139 75 6 324 459 147 274 6.60e-14 73.6
MsaG043943 MtrunA17_Chr1g0194481 44.286 70 38 1 328 397 158 226 2.69e-14 73.9
MsaG043943 MtrunA17_Chr6g0470161 43.299 97 48 2 397 486 24 120 3.52e-14 68.9
MsaG043943 MtrunA17_Chr4g0074001 37.037 108 55 3 293 388 312 418 4.93e-14 74.3
MsaG043943 MtrunA17_Chr7g0270191 38.542 96 57 2 301 396 119 212 5.51e-14 72.4
MsaG043943 MtrunA17_Chr8g0387981 41.379 87 45 2 303 383 103 189 5.84e-14 73.2
MsaG043943 MtrunA17_Chr4g0004911 52.174 69 31 2 321 388 176 243 1.16e-13 73.2
MsaG043943 MtrunA17_Chr4g0004911 44.262 61 33 1 328 388 336 395 1.47e-12 69.7
MsaG043943 MtrunA17_Chr5g0443131 50.000 62 30 1 328 389 157 217 1.23e-13 71.6
MsaG043943 MtrunA17_Chr4g0051881 49.180 61 30 1 328 388 178 237 1.58e-13 71.6
MsaG043943 MtrunA17_Chr7g0249301 47.619 63 32 1 328 390 292 353 3.63e-13 70.9
MsaG043943 MtrunA17_Chr7g0249301 47.541 61 30 2 328 388 62 120 1.24e-11 66.2
MsaG043943 MtrunA17_Chr3g0106491 50.000 66 31 2 327 392 197 260 3.83e-13 71.6
MsaG043943 MtrunA17_Chr3g0106491 43.548 62 34 1 328 389 365 425 2.61e-11 65.9
MsaG043943 MtrunA17_Chr3g0101131 41.791 67 38 1 328 394 415 480 4.45e-13 71.6
MsaG043943 MtrunA17_Chr3g0101131 48.529 68 33 2 321 388 236 301 1.04e-12 70.5
MsaG043943 MtrunA17_Chr5g0445311 41.791 67 38 1 328 394 364 429 4.66e-13 71.2
MsaG043943 MtrunA17_Chr5g0445311 48.438 64 31 2 325 388 197 258 3.79e-12 68.6
MsaG043943 MtrunA17_Chr3g0129321 45.161 62 33 1 328 389 118 178 5.91e-13 67.8
MsaG043943 MtrunA17_Chr4g0068541 38.571 70 42 1 328 397 208 276 8.06e-13 70.1
MsaG043943 MtrunA17_Chr2g0294231 44.615 65 35 1 328 392 203 266 1.07e-12 69.7
MsaG043943 MtrunA17_Chr5g0432161 53.226 62 27 2 327 388 243 302 1.29e-12 70.1
MsaG043943 MtrunA17_Chr5g0432161 44.444 63 34 1 328 390 421 482 1.51e-12 69.7
MsaG043943 MtrunA17_Chr8g0383251 43.939 66 36 1 323 388 103 167 1.54e-12 66.2
MsaG043943 MtrunA17_Chr7g0238921 33.099 142 88 4 250 385 40 180 2.31e-12 67.8
MsaG043943 MtrunA17_Chr5g0418781 40.230 87 50 2 299 385 96 180 2.33e-12 67.4
MsaG043943 MtrunA17_Chr2g0302391 41.667 60 35 0 329 388 212 271 2.51e-12 68.6
MsaG043943 MtrunA17_Chr3g0136961 41.772 79 43 1 328 406 138 213 2.76e-12 67.4
MsaG043943 MtrunA17_Chr1g0188941 42.623 61 34 1 328 388 51 110 4.28e-12 62.8
MsaG043943 MtrunA17_Chr3g0126391 37.209 86 45 3 306 383 106 190 4.42e-12 67.4
MsaG043943 MtrunA17_Chr3g0099641 33.708 89 47 1 300 388 235 311 5.16e-12 67.8
MsaG043943 MtrunA17_Chr4g0001361 41.026 78 45 1 328 404 162 239 8.08e-12 66.2
MsaG043943 MtrunA17_Chr4g0075201 30.435 115 65 3 276 388 226 327 4.09e-11 65.1
MsaG043943 MtrunA17_Chr5g0447701 46.667 60 31 1 329 388 253 311 4.31e-11 64.7
MsaG043943 MtrunA17_Chr2g0333851 41.935 62 36 0 328 389 169 230 4.65e-11 63.9
MsaG043943 MtrunA17_Chr1g0189391 42.529 87 46 4 317 401 85 169 6.14e-11 63.9
MsaG043943 MtrunA17_Chr7g0270831 40.000 60 36 0 329 388 217 276 7.97e-11 63.9
MsaG043943 MtrunA17_Chr8g0335811 34.906 106 64 4 290 394 117 218 9.32e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043943 AT5G52830.1 53.535 99 44 2 305 403 143 239 2.32e-28 115
MsaG043943 AT4G01250.1 37.931 145 79 5 260 402 66 201 2.90e-25 105
MsaG043943 AT4G23550.1 47.872 94 48 1 312 405 124 216 1.80e-22 98.2
MsaG043943 AT5G45050.4 51.613 93 43 2 305 397 1019 1109 1.87e-21 99.0
MsaG043943 AT5G45050.1 51.613 93 43 2 305 397 1158 1248 2.14e-21 98.6
MsaG043943 AT5G45050.2 51.613 93 43 2 305 397 1130 1220 2.34e-21 98.6
MsaG043943 AT2G34830.1 56.522 69 29 1 329 397 216 283 8.54e-20 92.0
MsaG043943 AT1G30650.1 56.522 69 29 1 329 397 218 285 8.73e-20 92.0
MsaG043943 AT2G34830.2 56.522 69 29 1 329 397 225 292 8.93e-20 92.0
MsaG043943 AT4G31550.1 52.308 65 31 0 329 393 247 311 2.89e-19 89.0
MsaG043943 AT4G31550.2 52.308 65 31 0 329 393 246 310 3.02e-19 89.0
MsaG043943 AT2G23320.1 56.140 57 25 0 329 385 241 297 1.82e-18 86.7
MsaG043943 AT2G24570.1 49.231 65 33 0 329 393 244 308 2.84e-18 86.3
MsaG043943 AT5G28650.1 51.515 66 32 0 329 394 263 328 4.44e-18 85.9
MsaG043943 AT1G29280.1 44.086 93 49 1 299 388 42 134 4.63e-18 84.3
MsaG043943 AT3G04670.1 51.515 66 32 0 329 394 263 328 5.94e-18 85.5
MsaG043943 AT4G24240.1 48.810 84 38 2 306 385 256 338 8.21e-18 85.1
MsaG043943 AT3G58710.2 51.471 68 33 0 326 393 68 135 9.23e-18 83.6
MsaG043943 AT3G58710.1 51.471 68 33 0 326 393 69 136 9.38e-18 83.6
MsaG043943 AT3G01080.3 47.436 78 36 3 324 401 201 273 3.48e-17 82.8
MsaG043943 AT3G01080.2 47.436 78 36 3 324 401 201 273 1.16e-16 82.8
MsaG043943 AT3G01080.2 47.541 61 31 1 328 388 344 403 2.65e-14 75.5
MsaG043943 AT3G01080.1 47.436 78 36 3 324 401 163 235 1.22e-16 82.4
MsaG043943 AT3G01080.1 47.541 61 31 1 328 388 306 365 3.35e-14 75.1
MsaG043943 AT2G30590.1 54.237 59 27 0 329 387 314 372 4.18e-16 80.5
MsaG043943 AT2G38470.1 50.000 80 36 3 309 388 167 242 5.42e-15 77.8
MsaG043943 AT2G38470.1 42.466 73 40 2 328 400 362 432 9.52e-13 70.9
MsaG043943 AT2G47260.1 37.391 115 66 3 301 411 148 260 5.64e-15 76.6
MsaG043943 AT2G03340.1 47.761 67 34 1 328 394 415 480 6.16e-15 77.8
MsaG043943 AT2G03340.1 48.571 70 34 2 324 393 246 313 7.98e-14 73.9
MsaG043943 AT3G62340.1 40.476 84 48 2 328 411 118 199 1.20e-14 74.7
MsaG043943 AT4G30935.1 35.780 109 57 3 292 388 283 390 2.91e-14 75.5
MsaG043943 AT2G21900.1 39.362 94 52 2 301 390 78 170 3.60e-14 71.6
MsaG043943 AT1G13960.1 40.816 98 48 4 309 401 205 297 5.09e-14 74.7
MsaG043943 AT1G13960.1 47.541 61 31 1 328 388 409 468 1.81e-13 73.2
MsaG043943 AT4G26440.1 45.946 74 38 2 326 399 176 247 5.52e-14 74.7
MsaG043943 AT4G26440.1 41.667 72 41 1 328 399 372 442 9.25e-12 67.8
MsaG043943 AT5G56270.1 34.194 155 78 5 326 458 271 423 5.60e-14 75.1
MsaG043943 AT5G56270.1 44.444 63 34 1 328 390 487 548 9.03e-13 71.2
MsaG043943 AT1G13960.2 47.436 78 36 3 324 401 72 144 1.09e-13 72.8
MsaG043943 AT1G13960.2 47.541 61 31 1 328 388 256 315 3.03e-13 71.6
MsaG043943 AT1G64000.1 38.095 84 48 3 303 385 90 170 1.71e-13 69.7
MsaG043943 AT2G30250.1 50.000 60 29 1 329 388 329 387 1.91e-13 72.4
MsaG043943 AT2G30250.1 48.387 62 30 2 327 388 165 224 1.02e-11 67.0
MsaG043943 AT5G43290.1 55.172 58 25 1 328 385 114 170 2.37e-13 70.9
MsaG043943 AT5G41570.1 40.000 85 48 3 303 385 71 154 5.33e-13 67.8
MsaG043943 AT5G07100.1 35.455 110 64 3 327 430 116 224 5.36e-13 70.1
MsaG043943 AT5G07100.1 40.580 69 40 1 328 396 234 301 2.41e-12 68.2
MsaG043943 AT2G37260.1 38.095 84 48 2 305 385 323 405 1.02e-12 70.5
MsaG043943 AT2G37260.1 49.254 67 32 2 324 390 161 225 4.87e-12 68.2
MsaG043943 AT2G04880.1 48.333 60 30 1 329 388 308 366 1.20e-12 70.5
MsaG043943 AT2G04880.2 48.333 60 30 1 329 388 284 342 1.30e-12 70.1
MsaG043943 AT1G80840.1 43.548 62 35 0 328 389 146 207 1.32e-12 68.9
MsaG043943 AT1G69310.4 39.773 88 51 2 299 386 119 204 1.33e-12 68.6
MsaG043943 AT1G69310.2 39.773 88 51 2 299 386 119 204 1.33e-12 68.6
MsaG043943 AT1G69310.1 39.773 88 51 2 299 386 119 204 1.33e-12 68.6
MsaG043943 AT2G40740.1 37.931 87 49 2 302 383 144 230 1.47e-12 68.6
MsaG043943 AT2G37260.3 38.095 84 48 2 305 385 243 325 1.59e-12 69.3
MsaG043943 AT2G37260.3 49.254 67 32 2 324 390 81 145 7.97e-12 67.0
MsaG043943 AT1G29860.1 43.478 69 38 1 328 396 136 203 1.62e-12 68.6
MsaG043943 AT1G55600.1 46.970 66 33 2 323 388 303 366 1.82e-12 69.7
MsaG043943 AT2G37260.2 38.095 84 48 2 305 385 241 323 1.83e-12 68.9
MsaG043943 AT2G37260.2 49.254 67 32 2 324 390 79 143 9.26e-12 67.0
MsaG043943 AT2G40740.3 37.931 87 49 2 302 383 153 239 1.86e-12 68.6
MsaG043943 AT5G07100.2 35.455 110 64 3 327 430 23 131 2.29e-12 66.6
MsaG043943 AT5G07100.2 40.580 69 40 1 328 396 141 208 7.67e-12 65.1
MsaG043943 AT5G07100.4 35.455 110 64 3 327 430 23 131 2.29e-12 66.6
MsaG043943 AT5G07100.4 40.580 69 40 1 328 396 141 208 7.67e-12 65.1
MsaG043943 AT5G07100.5 35.455 110 64 3 327 430 23 131 2.29e-12 66.6
MsaG043943 AT5G07100.5 40.580 69 40 1 328 396 141 208 7.67e-12 65.1
MsaG043943 AT5G07100.3 35.455 110 64 3 327 430 23 131 2.29e-12 66.6
MsaG043943 AT5G07100.3 40.580 69 40 1 328 396 141 208 7.67e-12 65.1
MsaG043943 AT5G26170.1 47.619 63 32 1 323 385 108 169 2.55e-12 65.9
MsaG043943 AT4G18170.1 41.026 78 44 2 308 385 153 228 2.61e-12 68.2
MsaG043943 AT4G26640.7 38.462 104 57 5 327 427 170 269 4.32e-12 68.6
MsaG043943 AT4G26640.7 40.984 61 35 1 328 388 341 400 1.69e-11 67.0
MsaG043943 AT4G26640.2 38.462 104 57 5 327 427 210 309 4.47e-12 68.9
MsaG043943 AT4G26640.2 40.984 61 35 1 328 388 381 440 1.67e-11 67.0
MsaG043943 AT5G45260.1 41.026 78 43 2 329 404 1209 1285 5.45e-12 68.9
MsaG043943 AT4G26640.1 38.462 104 57 5 327 427 138 237 5.95e-12 68.2
MsaG043943 AT4G26640.1 40.984 61 35 1 328 388 309 368 2.01e-11 66.6
MsaG043943 AT5G49520.1 44.828 58 31 1 328 385 221 277 6.84e-12 67.8
MsaG043943 AT4G26640.5 38.462 104 57 5 327 427 34 133 8.39e-12 67.4
MsaG043943 AT4G26640.5 40.984 61 35 1 328 388 205 264 2.06e-11 65.9
MsaG043943 AT4G26640.6 38.462 104 57 5 327 427 34 133 8.39e-12 67.4
MsaG043943 AT4G26640.6 40.984 61 35 1 328 388 205 264 2.06e-11 65.9
MsaG043943 AT4G26640.4 38.462 104 57 5 327 427 34 133 8.39e-12 67.4
MsaG043943 AT4G26640.4 40.984 61 35 1 328 388 205 264 2.06e-11 65.9
MsaG043943 AT4G26640.3 38.462 104 57 5 327 427 34 133 8.39e-12 67.4
MsaG043943 AT4G26640.3 40.984 61 35 1 328 388 205 264 2.06e-11 65.9
MsaG043943 AT2G46400.1 43.182 88 45 3 301 383 74 161 1.16e-11 65.9
MsaG043943 AT5G46350.1 45.902 61 32 1 328 388 183 242 1.35e-11 66.2
MsaG043943 AT2G44745.1 34.694 98 62 2 289 385 105 201 2.21e-11 63.9
MsaG043943 AT4G12020.6 32.800 125 74 5 295 413 430 550 5.39e-11 65.9
MsaG043943 AT4G12020.8 32.800 125 74 5 295 413 430 550 5.39e-11 65.9
MsaG043943 AT4G22070.4 40.000 60 36 0 329 388 298 357 8.89e-11 64.7
MsaG043943 AT4G22070.3 40.000 60 36 0 329 388 298 357 8.89e-11 64.7
MsaG043943 AT4G22070.2 40.000 60 36 0 329 388 298 357 8.89e-11 64.7
MsaG043943 AT4G22070.1 40.000 60 36 0 329 388 298 357 8.89e-11 64.7

Find 135 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGCTTAAGTGGGGTAAT+TGG 0.193333 8:-53641122 None:intergenic
ACTACAAATGTAGAAGTTTC+TGG 0.203501 8:+53643090 MsaT043943.1:CDS
AGTCTTTGATGACTTGTTTC+GGG 0.228480 8:-53643237 None:intergenic
GGTAGTGATGGTTGGGATAC+TGG 0.266171 8:-53641216 None:intergenic
GGAAGGGATGGTGGTGCTTG+AGG 0.267804 8:-53641261 None:intergenic
TCGGCTTAAGTGGGGTAATT+GGG 0.268846 8:-53641121 None:intergenic
GAAGGGATGGTGGTGCTTGA+GGG 0.285161 8:-53641260 None:intergenic
TGGATGTGGAGACCCTTTAA+TGG 0.287579 8:-53642037 None:intergenic
TGGAGGTGGAGTTTCAAAAT+TGG 0.294278 8:-53641003 None:intergenic
TTGAGGGGATATGATGTTAT+TGG 0.296773 8:-53641042 None:intergenic
AACGGCAACATCGCCATATT+AGG 0.297130 8:-53643317 None:intergenic
TGCGATTTCACTGCGGGCAT+GGG 0.316131 8:-53641678 None:intergenic
TAGTGAAGATGGTGGTAGTG+GGG 0.325049 8:-53641472 None:intergenic
AAACTTGGAATCGCCTAATA+TGG 0.330985 8:+53643304 MsaT043943.1:CDS
AGGGTGGCGAATGTGGTTGT+AGG 0.331360 8:-53641497 None:intergenic
CTCCAATGGACAATGATGAT+TGG 0.334060 8:+53640925 None:intergenic
CAAAATTTAAATCAAGAAAA+AGG 0.335426 8:+53641834 MsaT043943.1:CDS
TAGTCTTTGATGACTTGTTT+CGG 0.341304 8:-53643238 None:intergenic
GTAGTGATGGTAGGTATGAT+AGG 0.345611 8:-53641533 None:intergenic
GTAGTTGATTGCGCTTTCTC+AGG 0.345960 8:-53641754 None:intergenic
ACTTATGTTGTGACATATAA+AGG 0.361618 8:+53643161 MsaT043943.1:CDS
GGATATGATGTTATTGGTTG+TGG 0.366736 8:-53641036 None:intergenic
TTGCGATTTCACTGCGGGCA+TGG 0.375796 8:-53641679 None:intergenic
TACACTTCATTGTTACGGTT+TGG 0.377794 8:-53641644 None:intergenic
AGTTCTTACCGATACGTCTA+TGG 0.380944 8:+53643367 MsaT043943.1:CDS
ATGAAATCATTCGGCTTAAG+TGG 0.384871 8:-53641131 None:intergenic
AGGGATGGTGGTGCTTGAGG+GGG 0.385352 8:-53641258 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.385486 8:-53642036 None:intergenic
GTTGGGGTGGTAGTGATGGT+TGG 0.392907 8:-53641224 None:intergenic
GTTGTGGTGGTTAGAGTTGG+AGG 0.394427 8:-53641020 None:intergenic
GGTAGTGAAGATGGTGGTAG+TGG 0.395152 8:-53641474 None:intergenic
TTTGTTCGAGGTAGTAGAAA+TGG 0.396886 8:-53641776 None:intergenic
AAGGGATGGTGGTGCTTGAG+GGG 0.398824 8:-53641259 None:intergenic
AAACATGACACCTATGTTAG+AGG 0.407154 8:+53643430 MsaT043943.1:CDS
AGGGGTTGTTGTGGAATTGA+TGG 0.409578 8:-53641734 None:intergenic
AGAGGTGGGGATAGTGGTGT+TGG 0.411378 8:-53641432 None:intergenic
TCCAATGGACAATGATGATT+GGG 0.413894 8:+53640926 None:intergenic
TTGGTTGTGGTGGTTAGAGT+TGG 0.415176 8:-53641023 None:intergenic
CGCTTTCTCAGGGGTTGTTG+TGG 0.415304 8:-53641743 None:intergenic
GTTGGGATACTGGTGATAGT+AGG 0.415858 8:-53641206 None:intergenic
TTGGGGTGGTAGTGATGGTT+GGG 0.421087 8:-53641223 None:intergenic
CTACAAATGTAGAAGTTTCT+GGG 0.423709 8:+53643091 MsaT043943.1:CDS
TAGAAGTTTCTGGGATTGTT+CGG 0.428728 8:+53643100 MsaT043943.1:CDS
GGGTGGCGAATGTGGTTGTA+GGG 0.432234 8:-53641496 None:intergenic
GATGGTAGAGGTGGGGATAG+TGG 0.437887 8:-53641438 None:intergenic
TCAAGCTCTATGAAATCATT+CGG 0.442212 8:-53641140 None:intergenic
GGTAATGTGGGAAGGGATGG+TGG 0.443946 8:-53641270 None:intergenic
GATAGTAGGGGTGGGGATAG+TGG 0.445675 8:-53641192 None:intergenic
AGTGGTTGGGGTGGTAGTGA+TGG 0.446309 8:-53641228 None:intergenic
ACTTGAGCCTCGTTGCTCTC+TGG 0.448771 8:-53643347 None:intergenic
ACGAGCCTTCAGAAGATTCA+TGG 0.450159 8:+53641989 MsaT043943.1:CDS
TATGTTAGAGGATCTCTTGA+TGG 0.450163 8:+53643442 MsaT043943.1:CDS
TTGGGATACTGGTGATAGTA+GGG 0.453252 8:-53641205 None:intergenic
GAGGTGGGGATAGTGGTGTT+GGG 0.454610 8:-53641431 None:intergenic
AAACATACACTTCATTGTTA+CGG 0.454894 8:-53641649 None:intergenic
GGGGTGGGGATAGTGGTTGT+TGG 0.455018 8:-53641185 None:intergenic
TAGTGATGGTAGGTATGATA+GGG 0.457142 8:-53641532 None:intergenic
TGGGGCTGAGTTATTCTCCT+TGG 0.469030 8:-53641102 None:intergenic
TTAATCAGAACTCTGTTGTT+GGG 0.469555 8:+53643210 MsaT043943.1:CDS
AGGGGTAGTGGTGGTGAGGG+TGG 0.470018 8:-53641513 None:intergenic
AGTGAAGATGGTGGTAGTGG+GGG 0.472105 8:-53641471 None:intergenic
GTTAATCAGAACTCTGTTGT+TGG 0.473025 8:+53643209 MsaT043943.1:CDS
GGTGGTGAGGGTGGCGAATG+TGG 0.473258 8:-53641504 None:intergenic
TTATGCATTGGCAAAGAAGC+TGG 0.473516 8:+53643263 MsaT043943.1:CDS
GTAGTGAAGATGGTGGTAGT+GGG 0.473726 8:-53641473 None:intergenic
GGTGGTAATGTGGGAAGGGA+TGG 0.475750 8:-53641273 None:intergenic
TGATAGGGGTAGTGGTGGTG+AGG 0.478349 8:-53641517 None:intergenic
TAGTTGATTGCGCTTTCTCA+GGG 0.481392 8:-53641753 None:intergenic
TTGCGCAAATGTCAGAAACT+TGG 0.482429 8:+53643289 MsaT043943.1:CDS
TATGATGTTATTGGTTGTGG+TGG 0.483931 8:-53641033 None:intergenic
TCTAACATAGGTGTCATGTT+TGG 0.484082 8:-53643428 None:intergenic
GTTGGTTGCGATTTCACTGC+GGG 0.486497 8:-53641684 None:intergenic
TCATCAAAGCATGGAAGTGT+AGG 0.486908 8:-53641068 None:intergenic
GTTGTTGTTCGATGAGTGTT+GGG 0.487764 8:-53641355 None:intergenic
GGTTGTAGGGGTAGTGAAGA+TGG 0.488717 8:-53641483 None:intergenic
TACTGGTGATAGTAGGGGTG+GGG 0.488721 8:-53641199 None:intergenic
GGTAGGAGTAGTGGTTGGGG+TGG 0.493221 8:-53641237 None:intergenic
ATAGAGCACCACTATTGATC+TGG 0.499970 8:-53643472 None:intergenic
GTGGTGGTTAGAGTTGGAGG+TGG 0.500554 8:-53641017 None:intergenic
ACAGAGTTCTGATTAACTGC+TGG 0.506512 8:-53643203 None:intergenic
GATACTGGTGATAGTAGGGG+TGG 0.507596 8:-53641201 None:intergenic
TTGCTCTCTGGCTGATCTAA+CGG 0.509856 8:-53643335 None:intergenic
GGTGGTAGGAGTAGCAAAAG+TGG 0.510553 8:-53640982 None:intergenic
TGTTGTTGTTCGATGAGTGT+TGG 0.514177 8:-53641356 None:intergenic
GTTGTTGTGGAATTGATGGA+TGG 0.514803 8:-53641730 None:intergenic
TGATGGTATTGGTGGTAATG+TGG 0.520234 8:-53641283 None:intergenic
CGGCTTAAGTGGGGTAATTG+GGG 0.520319 8:-53641120 None:intergenic
GATAGGGGTAGTGGTGGTGA+GGG 0.522610 8:-53641516 None:intergenic
AGGTGGAGTTTCAAAATTGG+TGG 0.528008 8:-53641000 None:intergenic
GGTGTGAGCAGTGATGGTAG+AGG 0.528684 8:-53641450 None:intergenic
GTGAGCAGTGATGGTAGAGG+TGG 0.533809 8:-53641447 None:intergenic
TGTTGGGAAATTAGAAAAGG+TGG 0.536200 8:-53641339 None:intergenic
ATCAAGAGATCCTCTAACAT+AGG 0.542974 8:-53643440 None:intergenic
TGAGCAGTGATGGTAGAGGT+GGG 0.544379 8:-53641446 None:intergenic
ATTAGTCGTTGTAGTAGTGA+TGG 0.545537 8:-53641546 None:intergenic
GGTAGGTATGATAGGGGTAG+TGG 0.546289 8:-53641525 None:intergenic
CATCAAAGCATGGAAGTGTA+GGG 0.546665 8:-53641067 None:intergenic
GCGATTTCACTGCGGGCATG+GGG 0.547693 8:-53641677 None:intergenic
TGAAATCATTCGGCTTAAGT+GGG 0.547986 8:-53641130 None:intergenic
GATGGTATTGGTGGTAATGT+GGG 0.548603 8:-53641282 None:intergenic
GACTTCAACCAGATCAATAG+TGG 0.548651 8:+53643464 MsaT043943.1:CDS
TCCCAATCATCATTGTCCAT+TGG 0.550956 8:-53640927 None:intergenic
CATGCCCATGAATCTTCTGA+AGG 0.555237 8:-53641994 None:intergenic
GAGTGTTGGGAAATTAGAAA+AGG 0.562639 8:-53641342 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.562993 8:-53641294 None:intergenic
GGAGTTTCAAAATTGGTGGT+AGG 0.564205 8:-53640996 None:intergenic
GGTGGCGAATGTGGTTGTAG+GGG 0.565157 8:-53641495 None:intergenic
AGGTGGGGATAGTGGTGTTG+GGG 0.565500 8:-53641430 None:intergenic
CGAGCCTTCAGAAGATTCAT+GGG 0.566390 8:+53641990 MsaT043943.1:CDS
AGTGATGGTAGGTATGATAG+GGG 0.566864 8:-53641531 None:intergenic
CATGGCACACAAATGTCACT+TGG 0.574104 8:-53641957 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.584498 8:+53642040 MsaT043943.1:CDS
AAACTAAAATCATCAAAGCA+TGG 0.588328 8:-53641077 None:intergenic
TCAAAGACTAGATTATGCAT+TGG 0.588584 8:+53643251 MsaT043943.1:CDS
ATGAAAACTAGTGTTAGTGA+TGG 0.596957 8:-53641300 None:intergenic
AAGGCTCGTCTTCCCTCACA+TGG 0.597848 8:-53641975 None:intergenic
CACAACATAAGTGTTCTCAC+TGG 0.597849 8:-53643150 None:intergenic
CTTCAGAAGATTCATGGGCA+TGG 0.598976 8:+53641995 MsaT043943.1:CDS
ATACTGGTGATAGTAGGGGT+GGG 0.601151 8:-53641200 None:intergenic
TGCACAATCAAAACTAGTGT+TGG 0.602127 8:-53641702 None:intergenic
AGTGTTAGTGATGGTATTGG+TGG 0.606164 8:-53641291 None:intergenic
GTATTGGTGGTAATGTGGGA+AGG 0.610190 8:-53641278 None:intergenic
TATTGGTGGTAATGTGGGAA+GGG 0.622832 8:-53641277 None:intergenic
GACATTTGTGTGCCATGTGA+GGG 0.624856 8:+53641963 MsaT043943.1:CDS
CTTTGATGACTTGTTTCGGG+AGG 0.626610 8:-53643234 None:intergenic
TGGGATACTGGTGATAGTAG+GGG 0.637481 8:-53641204 None:intergenic
GTCGTTGTAGTAGTGATGGT+AGG 0.641331 8:-53641542 None:intergenic
TGACATTTGTGTGCCATGTG+AGG 0.643817 8:+53641962 MsaT043943.1:CDS
AGGTATGATAGGGGTAGTGG+TGG 0.649146 8:-53641522 None:intergenic
GAAATCATTCGGCTTAAGTG+GGG 0.658767 8:-53641129 None:intergenic
AGTTGATTGCGCTTTCTCAG+GGG 0.662828 8:-53641752 None:intergenic
TGTTGGTTGCGATTTCACTG+CGG 0.685999 8:-53641685 None:intergenic
TGTAGGGGTAGTGAAGATGG+TGG 0.689397 8:-53641480 None:intergenic
GAGCAGTGATGGTAGAGGTG+GGG 0.706368 8:-53641445 None:intergenic
AGATCAGCCAGAGAGCAACG+AGG 0.772309 8:+53643340 MsaT043943.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 53640930 53643502 53640930 ID=MsaG043943
Chr8 mRNA 53640930 53643502 53640930 ID=MsaT043943.1;Parent=MsaG043943
Chr8 exon 53640930 53641855 53640930 ID=MsaT043943.1.exon1;Parent=MsaT043943.1
Chr8 CDS 53640930 53641855 53640930 ID=cds.MsaT043943.1;Parent=MsaT043943.1
Chr8 exon 53641945 53642061 53641945 ID=MsaT043943.1.exon2;Parent=MsaT043943.1
Chr8 CDS 53641945 53642061 53641945 ID=cds.MsaT043943.1;Parent=MsaT043943.1
Chr8 exon 53643085 53643502 53643085 ID=MsaT043943.1.exon3;Parent=MsaT043943.1
Chr8 CDS 53643085 53643502 53643085 ID=cds.MsaT043943.1;Parent=MsaT043943.1
Gene Sequence

>MsaT043943.1

ATGGACAATGATGATTGGGATCTCTTTTCTATTGTTCGTAGGTGCAAAGCTACCACTTTTGCTACTCCTACCACCAATTTTGAAACTCCACCTCCAACTCTAACCACCACAACCAATAACATCATATCCCCTCAAAACCCTACACTTCCATGCTTTGATGATTTTAGTTTTACCAAGGAGAATAACTCAGCCCCAATTACCCCACTTAAGCCGAATGATTTCATAGAGCTTGACAAATTGATAATCAGTTTCAATCCAACAACCACTATCCCCACCCCTACTATCACCAGTATCCCAACCATCACTACCACCCCAACCACTACTCCTACCCCCTCAAGCACCACCATCCCTTCCCACATTACCACCAATACCATCACTAACACTAGTTTTCATGATTCCAATCAAAATTCCACCTTTTCTAATTTCCCAACACTCATCGAACAACAACAACTGCAACTAAATGAGTTTACTGAGCTAGAAAAAATGGTACTTAAATTCTACCCCAACACCACTATCCCCACCTCTACCATCACTGCTCACACCCCCACTACCACCATCTTCACTACCCCTACAACCACATTCGCCACCCTCACCACCACTACCCCTATCATACCTACCATCACTACTACAACGACTAATACTAGTGTTTGTAAAACAAATCAATATCTTACAATTTCTGACTATCAAATACTCGTCGAACAACAACAGAATCAACCAAACCGTAACAATGAAGTGTATGTTTCTGAACCCCATGCCCGCAGTGAAATCGCAACCAACACTAGTTTTGATTGTGCATTCAACCATCCATCAATTCCACAACAACCCCTGAGAAAGCGCAATCAACTACCATTTCTACTACCTCGAACAAATTCTAGATTTTTACCAAATACACATCCGAAAAAACCAAAATTTAAATCAAGAAAAAGAAAAAATAATAACCAAGTGACATTTGTGTGCCATGTGAGGGAAGACGAGCCTTCAGAAGATTCATGGGCATGGAAAAAATATGGAGAAAAACCCATTAAAGGGTCTCCACATCCAAGGAGCTACTACAAATGTAGAAGTTTCTGGGATTGTTCGGCAAAAAAACTAGTAGAGAAAAGCAAAACCAGTGAGAACACTTATGTTGTGACATATAAAGGAGAACACAATCATGAAAAACCAGCAGTTAATCAGAACTCTGTTGTTGGGACCTCCCGAAACAAGTCATCAAAGACTAGATTATGCATTGGCAAAGAAGCTGGATCTTGCGCAAATGTCAGAAACTTGGAATCGCCTAATATGGCGATGTTGCCGTTAGATCAGCCAGAGAGCAACGAGGCTCAAGTTCTTACCGATACGTCTATGGAGTTTTTGGGAAGCTCTGATGACGATGTCATTTTGATACCAAACATGACACCTATGTTAGAGGATCTCTTGATGGACTTCAACCAGATCAATAGTGGTGCTCTATTCCCTTAG
Protein sequence

>MsaT043943.1

MDNDDWDLFSIVRRCKATTFATPTTNFETPPPTLTTTTNNIISPQNPTLPCFDDFSFTKENNSAPITPLKPNDFIELDKLIISFNPTTTIPTPTITSIPTITTTPTTTPTPSSTTIPSHITTNTITNTSFHDSNQNSTFSNFPTLIEQQQLQLNEFTELEKMVLKFYPNTTIPTSTITAHTPTTTIFTTPTTTFATLTTTTPIIPTITTTTTNTSVCKTNQYLTISDYQILVEQQQNQPNRNNEVYVSEPHARSEIATNTSFDCAFNHPSIPQQPLRKRNQLPFLLPRTNSRFLPNTHPKKPKFKSRKRKNNNQVTFVCHVREDEPSEDSWAWKKYGEKPIKGSPHPRSYYKCRSFWDCSAKKLVEKSKTSENTYVVTYKGEHNHEKPAVNQNSVVGTSRNKSSKTRLCIGKEAGSCANVRNLESPNMAMLPLDQPESNEAQVLTDTSMEFLGSSDDDVILIPNMTPMLEDLLMDFNQINSGALFP*