AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043961

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043961 MtrunA17_Chr8g0368901 98.476 656 10 0 1 656 114 769 0.0 1343
MsaG043961 MtrunA17_Chr8g0368911 75.190 657 145 7 1 654 119 760 0.0 1016
MsaG043961 MtrunA17_Chr8g0369301 74.164 658 139 4 1 656 121 749 0.0 999
MsaG043961 MtrunA17_Chr1g0163051 73.895 475 120 3 182 655 187 658 0.0 751
MsaG043961 MtrunA17_Chr1g0163041 66.167 467 155 3 182 647 104 568 0.0 644
MsaG043961 MtrunA17_Chr6g0467381 50.441 567 243 10 102 644 12 564 0.0 555
MsaG043961 MtrunA17_Chr6g0467421 50.441 567 243 10 102 644 12 564 0.0 554
MsaG043961 MtrunA17_Chr6g0467531 57.928 473 176 8 181 644 106 564 0.0 553
MsaG043961 MtrunA17_Chr6g0467401 49.912 567 246 10 102 644 12 564 0.0 549
MsaG043961 MtrunA17_Chr3g0094881 49.822 562 253 9 105 655 95 638 0.0 540
MsaG043961 MtrunA17_Chr6g0467411 51.660 482 191 8 171 644 35 482 7.30e-165 481
MsaG043961 MtrunA17_Chr2g0306961 46.466 566 193 15 83 644 4 463 7.80e-157 460
MsaG043961 MtrunA17_Chr5g0399801 41.685 463 247 8 184 642 615 1058 1.47e-114 369
MsaG043961 MtrunA17_Chr3g0080761 40.211 475 254 12 184 643 174 633 8.20e-110 345
MsaG043961 MtrunA17_Chr4g0003501 42.424 462 242 10 184 642 484 924 9.68e-110 353
MsaG043961 MtrunA17_Chr7g0255181 41.631 466 246 13 184 644 236 680 7.53e-109 344
MsaG043961 MtrunA17_Chr4g0003511 40.781 461 253 8 184 642 579 1021 1.33e-108 352
MsaG043961 MtrunA17_Chr8g0354821 39.248 479 254 11 184 645 201 659 1.82e-105 335
MsaG043961 MtrunA17_Chr6g0476111 38.223 484 264 13 184 645 196 666 1.22e-91 298
MsaG043961 MtrunA17_Chr7g0252211 37.938 485 265 13 184 645 258 729 4.31e-91 298
MsaG043961 MtrunA17_Chr6g0476121 38.017 484 265 13 184 645 145 615 2.42e-89 291
MsaG043961 MtrunA17_Chr7g0232811 31.733 479 227 17 187 642 107 508 6.61e-62 215
MsaG043961 MtrunA17_Chr1g0170651 32.636 478 273 12 180 642 77 520 2.41e-56 201
MsaG043961 MtrunA17_Chr7g0232981 30.998 471 233 15 181 642 98 485 3.75e-56 199
MsaG043961 MtrunA17_Chr7g0232841 30.000 480 220 19 187 642 110 497 6.47e-49 179
MsaG043961 MtrunA17_Chr8g0357391 59.559 136 51 3 73 207 2 134 6.37e-42 150
MsaG043961 MtrunA17_Chr8g0373821 30.645 186 115 2 458 642 343 515 1.77e-21 98.6
MsaG043961 MtrunA17_Chr5g0403241 32.020 203 117 6 459 656 1310 1496 2.49e-20 96.3
MsaG043961 MtrunA17_Chr1g0183321 26.059 307 155 10 394 644 430 720 1.14e-19 93.6
MsaG043961 MtrunA17_Chr1g0190311 25.466 322 159 10 384 644 317 618 9.88e-19 90.5
MsaG043961 MtrunA17_Chr4g0075321 29.630 189 111 3 460 642 868 1040 5.22e-18 88.6
MsaG043961 MtrunA17_Chr7g0232861 37.086 151 80 6 187 336 106 242 7.22e-18 83.6
MsaG043961 MtrunA17_Chr5g0448331 37.342 158 85 4 212 364 267 415 2.13e-16 83.2
MsaG043961 MtrunA17_Chr7g0262001 23.659 317 156 9 390 644 384 676 3.53e-16 82.4
MsaG043961 MtrunA17_Chr8g0389931 36.076 158 87 4 212 364 261 409 1.11e-15 80.9
MsaG043961 MtrunA17_Chr7g0232761 29.310 232 123 8 135 343 53 266 1.66e-14 74.3
MsaG043961 MtrunA17_Chr3g0143561 34.161 161 92 4 212 372 187 333 1.74e-13 72.8
MsaG043961 MtrunA17_Chr7g0232751 45.763 59 32 0 585 643 31 89 9.85e-13 65.5
MsaG043961 MtrunA17_Chr6g0460921 28.507 221 130 8 438 655 215 410 1.13e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043961 AT4G13460.1 60.894 537 187 9 121 655 134 649 0.0 661
MsaG043961 AT4G13460.2 60.894 537 187 9 121 655 134 649 0.0 661
MsaG043961 AT2G33290.1 63.169 486 172 5 171 655 171 650 0.0 652
MsaG043961 AT2G33290.3 63.169 486 172 5 171 655 171 650 0.0 652
MsaG043961 AT2G33290.4 63.169 486 172 5 171 655 171 650 0.0 652
MsaG043961 AT2G33290.2 63.169 486 172 5 171 655 171 650 0.0 652
MsaG043961 AT2G22740.2 38.333 480 272 9 175 645 298 762 9.38e-102 328
MsaG043961 AT2G22740.1 38.333 480 272 9 175 645 298 762 9.38e-102 328
MsaG043961 AT2G35160.5 36.447 546 292 14 110 642 224 727 5.43e-101 325
MsaG043961 AT2G35160.2 36.697 545 292 15 110 642 260 763 1.43e-100 325
MsaG043961 AT2G35160.3 36.697 545 292 15 110 642 260 763 1.43e-100 325
MsaG043961 AT2G35160.1 36.697 545 292 15 110 642 260 763 1.43e-100 325
MsaG043961 AT2G35160.4 36.697 545 292 15 110 642 260 763 1.43e-100 325
MsaG043961 AT1G73100.1 41.020 451 243 13 204 645 204 640 1.09e-99 319
MsaG043961 AT5G04940.2 37.996 479 255 12 184 645 188 641 9.51e-98 314
MsaG043961 AT5G04940.1 37.996 479 255 12 184 645 188 641 9.51e-98 314
MsaG043961 AT5G13960.1 37.552 482 270 16 184 645 126 596 1.06e-87 286
MsaG043961 AT5G13960.2 37.844 473 263 16 193 645 1 462 4.20e-86 278
MsaG043961 AT2G24740.1 37.445 454 236 17 205 645 307 725 2.20e-72 249
MsaG043961 AT1G17770.1 32.456 456 278 13 204 645 223 662 6.13e-69 238
MsaG043961 AT5G47150.1 41.989 181 96 4 184 364 153 324 3.00e-31 125
MsaG043961 AT5G47160.1 30.387 362 205 11 12 360 80 407 1.48e-27 116
MsaG043961 AT5G47160.3 30.387 362 205 11 12 360 80 407 1.48e-27 116
MsaG043961 AT5G47160.2 30.387 362 205 11 12 360 80 407 1.48e-27 116
MsaG043961 AT2G23740.3 34.043 188 104 5 461 643 1184 1356 5.52e-24 108
MsaG043961 AT2G23740.6 34.043 188 104 5 461 643 1184 1356 5.52e-24 108
MsaG043961 AT2G23740.2 34.043 188 104 5 461 643 1184 1356 5.52e-24 108
MsaG043961 AT2G23740.1 34.043 188 104 5 461 643 1177 1349 5.70e-24 108
MsaG043961 AT2G05900.1 36.782 174 102 6 249 422 26 191 3.91e-20 92.0
MsaG043961 AT3G03750.2 27.899 276 157 8 392 643 66 323 4.78e-17 83.6
MsaG043961 AT2G23740.4 34.247 146 76 5 461 601 1184 1314 9.11e-17 85.5
MsaG043961 AT2G23740.5 34.247 146 76 5 461 601 1184 1314 9.11e-17 85.5
MsaG043961 AT1G57820.1 36.047 172 86 6 208 364 264 426 3.95e-16 82.8
MsaG043961 AT1G57820.3 36.047 172 86 6 208 364 264 426 3.95e-16 82.8
MsaG043961 AT1G57820.2 36.095 169 87 6 208 364 264 423 8.99e-16 81.6
MsaG043961 AT5G39550.1 35.465 172 87 6 208 364 249 411 4.09e-15 79.3
MsaG043961 AT3G04380.2 23.779 307 164 9 393 644 118 409 6.18e-15 78.2
MsaG043961 AT3G04380.1 23.779 307 164 9 393 644 145 436 6.50e-15 78.2
MsaG043961 AT3G04380.4 23.779 307 164 9 393 644 145 436 7.02e-15 78.2
MsaG043961 AT3G04380.3 23.779 307 164 9 393 644 129 420 7.06e-15 78.2
MsaG043961 AT1G66040.1 35.443 158 79 4 208 351 249 397 7.99e-15 78.6
MsaG043961 AT3G04380.6 23.453 307 165 9 393 644 118 409 8.32e-15 77.8
MsaG043961 AT1G66050.1 35.443 158 79 4 208 351 249 397 9.20e-15 78.2
MsaG043961 AT3G04380.5 23.779 307 164 9 393 644 102 393 9.25e-15 77.4
MsaG043961 AT1G04050.2 24.679 312 151 10 394 644 409 697 1.31e-14 77.8
MsaG043961 AT1G04050.1 24.679 312 151 10 394 644 409 697 1.31e-14 77.8
MsaG043961 AT1G04050.3 24.679 312 151 10 394 644 305 593 1.59e-14 77.4
MsaG043961 AT5G43990.16 25.157 318 139 13 394 644 375 660 1.38e-13 74.7
MsaG043961 AT5G43990.5 25.157 318 139 13 394 644 375 660 1.38e-13 74.7
MsaG043961 AT5G43990.17 25.157 318 139 13 394 644 375 660 1.38e-13 74.7
MsaG043961 AT5G43990.14 25.157 318 139 13 394 644 375 660 1.38e-13 74.7
MsaG043961 AT5G43990.3 25.157 318 139 13 394 644 395 680 1.65e-13 74.3
MsaG043961 AT5G43990.11 25.157 318 139 13 394 644 395 680 1.65e-13 74.3
MsaG043961 AT5G43990.1 25.157 318 139 13 394 644 395 680 1.65e-13 74.3
MsaG043961 AT5G43990.4 25.157 318 139 13 394 644 395 680 1.65e-13 74.3
MsaG043961 AT5G43990.15 25.157 318 139 13 394 644 398 683 1.96e-13 74.3
MsaG043961 AT5G43990.10 25.157 318 139 13 394 644 398 683 1.96e-13 74.3
MsaG043961 AT5G43990.9 25.157 318 139 13 394 644 418 703 2.48e-13 73.9
MsaG043961 AT5G43990.2 25.157 318 139 13 394 644 418 703 2.48e-13 73.9
MsaG043961 AT5G43990.7 25.157 318 139 13 394 644 306 591 4.05e-13 73.2
MsaG043961 AT5G43990.8 25.157 318 139 13 394 644 326 611 4.69e-13 72.8
MsaG043961 AT5G43990.12 25.157 318 139 13 394 644 326 611 4.69e-13 72.8
MsaG043961 AT5G43990.6 25.157 318 139 13 394 644 326 611 4.69e-13 72.8
MsaG043961 AT5G43990.13 25.157 318 139 13 394 644 326 611 4.69e-13 72.8
MsaG043961 AT1G57800.1 34.839 155 77 6 208 347 276 421 3.11e-12 70.1
MsaG043961 AT4G08590.2 33.728 169 85 6 208 364 224 377 1.74e-11 67.4
MsaG043961 AT4G08590.4 35.000 160 76 6 208 356 224 366 5.44e-11 65.5
MsaG043961 AT4G08590.5 35.000 160 76 6 208 356 224 366 5.44e-11 65.5
MsaG043961 AT4G08590.1 35.374 147 70 5 208 343 224 356 9.54e-11 65.1

Find 210 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTCAACCCTCCTAATT+TGG 0.129688 8:-53856679 MsaT043961.1:CDS
TGCTGTTTCAATGGAGAATT+TGG 0.135730 8:-53856724 MsaT043961.1:CDS
GCTAGGGAGATTAAGAAAAT+TGG 0.140750 8:-53855631 MsaT043961.1:CDS
AATTAGGAGGGTTGAGATTT+AGG 0.220689 8:+53856682 None:intergenic
GCAAATTTGTGATCAGAAAT+TGG 0.236057 8:-53855890 MsaT043961.1:CDS
GTTTGATCACAACAATTTAA+TGG 0.253927 8:-53854915 MsaT043961.1:CDS
GCTACCAGTGTGATTGTTTC+GGG 0.257038 8:-53855997 MsaT043961.1:CDS
AAGCAAACCCTGAAGATTAT+AGG 0.291078 8:+53855369 None:intergenic
AATCTCAACCCTCCTAATTT+GGG 0.291219 8:-53856678 MsaT043961.1:CDS
GAGAGTAGGGTGGCGGCTGC+GGG 0.298883 8:-53856279 MsaT043961.1:CDS
AGAGTTTCCCTATAATCTTC+AGG 0.299770 8:-53855377 MsaT043961.1:CDS
TAGGTCCTTGGACCTTATTC+AGG 0.306366 8:-53855212 MsaT043961.1:CDS
CGCAGACTGGTTGGGGAGTT+AGG 0.311416 8:-53855231 MsaT043961.1:CDS
AGGATGGAATTGTGTGTAAT+TGG 0.313895 8:-53856090 MsaT043961.1:CDS
TCAATGGAGAATTTGGTTAA+TGG 0.317973 8:-53856717 MsaT043961.1:CDS
GGAGAGTAGGGTGGCGGCTG+CGG 0.326527 8:-53856280 MsaT043961.1:CDS
ATATCATTAAATAGCCTAAT+AGG 0.326852 8:+53855547 None:intergenic
ACACATTCGGACTCGAACTA+TGG 0.329088 8:+53854954 None:intergenic
TGCAACACGATTCCGACAAT+TGG 0.331483 8:+53855294 None:intergenic
GGGTGGTTATGAGGATGATA+TGG 0.333570 8:-53855977 MsaT043961.1:CDS
TGCTACCAGTGTGATTGTTT+CGG 0.335460 8:-53855998 MsaT043961.1:CDS
GAAGGGAGCGCGTCCGGTTC+TGG 0.342829 8:-53855793 MsaT043961.1:CDS
TATGATGGACGCACATAATC+AGG 0.344984 8:+53855046 None:intergenic
AGCCTTTATCTGCGAGTATA+CGG 0.345585 8:-53855185 MsaT043961.1:CDS
CAATGCTTCTGCTGTTTCAA+TGG 0.352780 8:-53856733 MsaT043961.1:CDS
GGCGGCTGCGGGGTTGGAAG+GGG 0.353649 8:-53856268 MsaT043961.1:CDS
AAACCGATTAGGATATATTA+AGG 0.354680 8:+53855111 None:intergenic
TCGAGCAATTGTTGTTGTTC+CGG 0.358474 8:-53856616 MsaT043961.1:CDS
ATCGGATACGTGGTGATATG+AGG 0.358773 8:-53856212 MsaT043961.1:CDS
TTGGGAGGACAGGAGCAAAA+AGG 0.359779 8:+53855313 None:intergenic
TAGGTCTGCGCAGACTGGTT+GGG 0.361131 8:-53855239 MsaT043961.1:CDS
TGGTTTGATGTAGGAAAGTC+TGG 0.364269 8:-53855727 MsaT043961.1:CDS
TAGGGTGGCGGCTGCGGGGT+TGG 0.365879 8:-53856274 MsaT043961.1:CDS
AGCAAACCCTGAAGATTATA+GGG 0.370075 8:+53855370 None:intergenic
TGTATAGGATTGTTGATTGT+TGG 0.370164 8:-53855747 MsaT043961.1:CDS
GGCATAGGTTGGAAGTGTTT+AGG 0.373570 8:-53855258 MsaT043961.1:CDS
CTAGGGAGATTAAGAAAATT+GGG 0.377655 8:-53855630 MsaT043961.1:CDS
AGAGAACTCAGCTTAGATTA+TGG 0.379852 8:-53854848 MsaT043961.1:CDS
TGAGAGTTGGTGAGGAGAGT+AGG 0.379963 8:-53856293 MsaT043961.1:CDS
GGCTGCTGAAGTTGCGGTTA+TGG 0.382507 8:-53856325 MsaT043961.1:CDS
ACTCCTTAATATATCCTAAT+CGG 0.383342 8:-53855114 MsaT043961.1:CDS
TTCAGATAGATGGGCAGAAT+GGG 0.385780 8:-53855089 MsaT043961.1:CDS
TTGGAAAGGAGTATGCATTA+TGG 0.387749 8:-53855850 MsaT043961.1:CDS
AACATAAACCTTACCAGAAC+CGG 0.388647 8:+53855780 None:intergenic
GTGGCGGCTGCGGGGTTGGA+AGG 0.388881 8:-53856270 MsaT043961.1:CDS
TAAAGGCTCCGGCCTGAATA+AGG 0.390483 8:+53855200 None:intergenic
TGAAACAGCAGAAGCATTGT+TGG 0.391424 8:+53856737 None:intergenic
GAGAGTTGGTGAGGAGAGTA+GGG 0.392298 8:-53856292 MsaT043961.1:CDS
AGAGGGAGAATGTTCCTATT+AGG 0.395044 8:-53855561 MsaT043961.1:CDS
ACCCGTATACTCGCAGATAA+AGG 0.399767 8:+53855183 None:intergenic
GAAGGCGTCGTGGTCGTAAT+CGG 0.400675 8:-53856230 MsaT043961.1:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.403279 8:-53855772 MsaT043961.1:CDS
TCGGAATCGTGTTGCACAAA+AGG 0.405257 8:-53855287 MsaT043961.1:CDS
GCTCCTGTCCTCCCAATTGT+CGG 0.405346 8:-53855306 MsaT043961.1:CDS
TGCATTATGGAATCGAGGTT+AGG 0.408570 8:-53855837 MsaT043961.1:CDS
CATCTATCTGAAAACCGATT+AGG 0.409004 8:+53855100 None:intergenic
GATGTAGGAAAGTCTGGTTT+TGG 0.420111 8:-53855721 MsaT043961.1:CDS
ATGTTTATGATGGATTGTAT+AGG 0.423249 8:-53855762 MsaT043961.1:CDS
AATTGAGGGGCAGGCTAAGA+TGG 0.423297 8:-53855674 MsaT043961.1:CDS
TCCTCCTTTAGATTATTCTC+TGG 0.423416 8:-53855014 MsaT043961.1:CDS
TTATGAGGATGATATGGAAT+TGG 0.423427 8:-53855971 MsaT043961.1:CDS
GGTGGGCGGAAACCTTGCTT+TGG 0.433844 8:-53855869 MsaT043961.1:CDS
TGGCGGCTGCGGGGTTGGAA+GGG 0.434291 8:-53856269 MsaT043961.1:CDS
AGACGGCAGTTGCGGTGGTT+AGG 0.437401 8:-53856584 MsaT043961.1:CDS
CGGAATCGTGTTGCACAAAA+GGG 0.437967 8:-53855286 MsaT043961.1:CDS
GGAGGGTTGAGATTTAGGTC+AGG 0.438104 8:+53856687 None:intergenic
AATGGCTGATGAGAGGAAAT+TGG 0.439250 8:-53856418 MsaT043961.1:CDS
CCTAAAAGTAGGTGCTCAAA+TGG 0.440141 8:-53856030 MsaT043961.1:CDS
AGGTCTGCGCAGACTGGTTG+GGG 0.451382 8:-53855238 MsaT043961.1:CDS
GGAGGAATTGAGGGATATGA+TGG 0.453245 8:+53855031 None:intergenic
AAGTATGAAGGGAGCGCGTC+CGG 0.454798 8:-53855799 MsaT043961.1:CDS
GAGTTTCCCTATAATCTTCA+GGG 0.462595 8:-53855376 MsaT043961.1:CDS
TTTCAGATAGATGGGCAGAA+TGG 0.462629 8:-53855090 MsaT043961.1:CDS
ATAATCTAAAGGAGGAATTG+AGG 0.464219 8:+53855021 None:intergenic
GAGGGTTGAGATTTAGGTCA+GGG 0.466746 8:+53856688 None:intergenic
GCCTTTATCTGCGAGTATAC+GGG 0.471911 8:-53855184 MsaT043961.1:CDS
ACAATCACACTGGTAGCAAT+TGG 0.473249 8:+53856003 None:intergenic
GATTGTTTCGGGTGGTTATG+AGG 0.473428 8:-53855986 MsaT043961.1:CDS
GTTGATTGTTGGTTTGATGT+AGG 0.478459 8:-53855736 MsaT043961.1:CDS
TATAAGCTATGGAGAATTGA+GGG 0.481089 8:-53855688 MsaT043961.1:CDS
GCATTATGGAATCGAGGTTA+GGG 0.483468 8:-53855836 MsaT043961.1:CDS
GCAACACGATTCCGACAATT+GGG 0.485278 8:+53855295 None:intergenic
TAATCTAAAGGAGGAATTGA+GGG 0.485316 8:+53855022 None:intergenic
TGTGGCTGTACCGCGATAAG+AGG 0.486843 8:-53856161 MsaT043961.1:CDS
CATCAGAGTGGGAATGCTAC+TGG 0.491035 8:-53855457 MsaT043961.1:CDS
AGGATAGTTGGACCGATACC+TGG 0.491184 8:-53856141 MsaT043961.1:CDS
TTAGGTCTGCGCAGACTGGT+TGG 0.492761 8:-53855240 MsaT043961.1:CDS
GTGTTGCACAAAAGGGGATC+AGG 0.493268 8:-53855279 MsaT043961.1:CDS
AATTCTTCTCCTGCCCTCCA+TGG 0.493649 8:+53855920 None:intergenic
GGAGATTAAGAAAATTGGGT+TGG 0.494139 8:-53855626 MsaT043961.1:CDS
TACCGCGATAAGAGGATAGT+TGG 0.495892 8:-53856153 MsaT043961.1:CDS
GGAGATTGGCTGAGGAGAGG+AGG 0.496998 8:-53856368 MsaT043961.1:CDS
TTATGATTCGCTTCGTGTGT+TGG 0.499962 8:-53856463 MsaT043961.1:CDS
GAGCGCGTCCGGTTCTGGTA+AGG 0.500524 8:-53855788 MsaT043961.1:CDS
CCCTCACAAGCTCCATCACC+TGG 0.504110 8:+53856550 None:intergenic
TGTGTGGAGGCTTGTACTGA+TGG 0.507598 8:-53855427 MsaT043961.1:CDS
GGCAGAAATGAGAGTTGGTG+AGG 0.507846 8:-53856301 MsaT043961.1:CDS
TGCTGTTCTCAAAGAGGCTA+GGG 0.508141 8:-53855647 MsaT043961.1:CDS
GGAAGAGAGGAGATTGGCTG+AGG 0.508243 8:-53856376 MsaT043961.1:CDS
CTACACTGGCCATGGAGGGC+AGG 0.508667 8:-53855929 MsaT043961.1:CDS
GAAGAAAGCAAGCCAGGTGA+TGG 0.510995 8:-53856562 MsaT043961.1:CDS
ACTGCCGTCTCTTGTTTCTC+CGG 0.514909 8:+53856597 None:intergenic
GCATACTCCTTTCCAAAGCA+AGG 0.516726 8:+53855857 None:intergenic
ACATACTGAACAAACACATT+CGG 0.516901 8:+53854941 None:intergenic
GGTTAGGAAGAAAGCAAGCC+AGG 0.517818 8:-53856568 MsaT043961.1:CDS
GAGTTTATAAGTATAAGCTA+TGG 0.519577 8:-53855699 MsaT043961.1:CDS
AGGGTTTGCTTGTGAGAGGG+AGG 0.526065 8:-53855357 MsaT043961.1:CDS
GCCAGGTGATGGAGCTTGTG+AGG 0.527540 8:-53856551 MsaT043961.1:CDS
TCATGTTATGCTATATGCAA+TGG 0.532362 8:-53854888 MsaT043961.1:CDS
TTGGAAGGGGAGATGTCGAA+TGG 0.534181 8:-53856255 MsaT043961.1:CDS
GTGTTTAGGTCTGCGCAGAC+TGG 0.534626 8:-53855244 MsaT043961.1:CDS
TTCAGGGTTTGCTTGTGAGA+GGG 0.535517 8:-53855360 MsaT043961.1:CDS
GTGCTGTTCTCAAAGAGGCT+AGG 0.536391 8:-53855648 MsaT043961.1:CDS
TCCTTGGACCTTATTCAGGC+CGG 0.537512 8:-53855208 MsaT043961.1:CDS
CAGATAGATGGGCAGAATGG+GGG 0.539870 8:-53855087 MsaT043961.1:CDS
ATTCGTGGGATGAAGTATGA+AGG 0.541112 8:-53855811 MsaT043961.1:CDS
AGATAGATGGGCAGAATGGG+GGG 0.541221 8:-53855086 MsaT043961.1:CDS
AGAAGAGAATGGCTGATGAG+AGG 0.541523 8:-53856425 MsaT043961.1:CDS
AAAGGGGATCAGGCATAGGT+TGG 0.541770 8:-53855269 MsaT043961.1:CDS
TTGATTACCTACCTAAAAGT+AGG 0.543148 8:-53856041 MsaT043961.1:CDS
AGTTTGTGTTTCATCAGAGT+GGG 0.543394 8:-53855468 MsaT043961.1:CDS
ATCGAGGTTAGGGTTATTCG+TGG 0.546312 8:-53855826 MsaT043961.1:CDS
TGGTTGGGGAGTTAGGTCCT+TGG 0.546847 8:-53855224 MsaT043961.1:CDS
CTGATCACAAATTTGCCTTG+AGG 0.547153 8:+53855897 None:intergenic
TGAGGGCTGCGGCGTTAATG+AGG 0.548073 8:-53856194 MsaT043961.1:CDS
TGCTACTGGCTGTGAATGTG+TGG 0.548302 8:-53855443 MsaT043961.1:CDS
ATTCCGACAATTGGGAGGAC+AGG 0.549040 8:+53855303 None:intergenic
GGGAGATGTCGAATGGAAGA+AGG 0.549164 8:-53856248 MsaT043961.1:CDS
CTTCAGGGTTTGCTTGTGAG+AGG 0.549559 8:-53855361 MsaT043961.1:CDS
GGAAGATACTCATAATAAAG+AGG 0.551468 8:+53855508 None:intergenic
AGAGGAGATTGGCTGAGGAG+AGG 0.552238 8:-53856371 MsaT043961.1:CDS
CAGTTTGTGTTTCATCAGAG+TGG 0.553822 8:-53855469 MsaT043961.1:CDS
GTATAAGCTATGGAGAATTG+AGG 0.554921 8:-53855689 MsaT043961.1:CDS
AAGTTGCTGTGGAGGAAGAG+AGG 0.555420 8:-53856389 MsaT043961.1:CDS
ACATCCAGAGAATAATCTAA+AGG 0.560417 8:+53855010 None:intergenic
TGTTGATATTTCACACAAGA+GGG 0.562610 8:-53855578 MsaT043961.1:CDS
GCTATGGAGAATTGAGGGGC+AGG 0.563295 8:-53855683 MsaT043961.1:CDS
TGTTGAGGAGGAGAAGAGAA+TGG 0.563959 8:-53856436 MsaT043961.1:CDS
TGGTGAGGAGAGTAGGGTGG+CGG 0.564313 8:-53856286 MsaT043961.1:CDS
GTTATAATCTACACTGGCCA+TGG 0.564532 8:-53855937 MsaT043961.1:CDS
GCGGAAACCTTGCTTTGGAA+AGG 0.566531 8:-53855864 MsaT043961.1:CDS
GGCATCGCAGACGAGTGGAC+AGG 0.568359 8:-53854827 MsaT043961.1:CDS
GGCTGCTGAAGTTGCTGTGG+AGG 0.569644 8:-53856397 MsaT043961.1:CDS
CCAGGTGATGGAGCTTGTGA+GGG 0.570883 8:-53856550 MsaT043961.1:CDS
AGAGTAGGGTGGCGGCTGCG+GGG 0.572127 8:-53856278 MsaT043961.1:CDS
ATGGAGGCAGAAATGAGAGT+TGG 0.572474 8:-53856306 MsaT043961.1:CDS
AGAGGAGGTTGACTGAGGAG+AGG 0.572711 8:-53856353 MsaT043961.1:CDS
ATTTGTGATCAGAAATTGGT+GGG 0.573131 8:-53855886 MsaT043961.1:CDS
CTGTTGATATTTCACACAAG+AGG 0.573952 8:-53855579 MsaT043961.1:CDS
ATTGAGGGGCAGGCTAAGAT+GGG 0.574785 8:-53855673 MsaT043961.1:CDS
GAAACAAGAGACGGCAGTTG+CGG 0.574901 8:-53856592 MsaT043961.1:CDS
TCAGATAGATGGGCAGAATG+GGG 0.578282 8:-53855088 MsaT043961.1:CDS
ATTGATTCTGAGCCGTGTGA+GGG 0.579164 8:-53856645 MsaT043961.1:CDS
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8:-53856529 MsaT043961.1:CDS
ATCTCAACCCTCCTAATTTG+GGG 0.582254 8:-53856677 MsaT043961.1:CDS
TTGCATATGCAGATACAAGC+TGG 0.583868 8:-53856066 MsaT043961.1:CDS
ATTGGCTGCTGAAGTTGCTG+TGG 0.584124 8:-53856400 MsaT043961.1:CDS
TCGGATACGTGGTGATATGA+GGG 0.590165 8:-53856211 MsaT043961.1:CDS
AGTTGGTGAGGAGAGTAGGG+TGG 0.593690 8:-53856289 MsaT043961.1:CDS
CCGATACCTGGAGTTTATGT+TGG 0.594828 8:-53856129 MsaT043961.1:CDS
TCGTGTGTTGGCGAGTGTTG+AGG 0.596528 8:-53856451 MsaT043961.1:CDS
CACAAAAGGGGATCAGGCAT+AGG 0.597403 8:-53855273 MsaT043961.1:CDS
TGTGGAGGAAGAGAGGAGAT+TGG 0.599002 8:-53856382 MsaT043961.1:CDS
ACATCACCAACATAAACTCC+AGG 0.599500 8:+53856123 None:intergenic
GCTCGATCATTTCCCTCACA+CGG 0.600129 8:+53856633 None:intergenic
TCAACAGAAAGACAATACAT+AGG 0.601273 8:+53855595 None:intergenic
AGCTGAGTTCTCTCAGAGGA+GGG 0.602912 8:+53854858 None:intergenic
CTCTGATGAAACACAAACTG+CGG 0.605398 8:+53855472 None:intergenic
ACAAGAGACGGCAGTTGCGG+TGG 0.607147 8:-53856589 MsaT043961.1:CDS
TATTGATTCTGAGCCGTGTG+AGG 0.608883 8:-53856646 MsaT043961.1:CDS
TAATCTACACTGGCCATGGA+GGG 0.614118 8:-53855933 MsaT043961.1:CDS
CCAACATAAACTCCAGGTAT+CGG 0.615137 8:+53856129 None:intergenic
TACTGGCTGTGAATGTGTGG+AGG 0.615161 8:-53855440 MsaT043961.1:CDS
ACGTGGTGATATGAGGGCTG+CGG 0.615445 8:-53856205 MsaT043961.1:CDS
AAGCTGAGTTCTCTCAGAGG+AGG 0.616231 8:+53854857 None:intergenic
TGCTGAAGTTGCGGTTATGG+AGG 0.617182 8:-53856322 MsaT043961.1:CDS
GTTGACTGAGGAGAGGATGT+TGG 0.618231 8:-53856346 MsaT043961.1:CDS
TCCGGCCTGAATAAGGTCCA+AGG 0.620502 8:+53855207 None:intergenic
ATTATGGCATCGCAGACGAG+TGG 0.626133 8:-53854832 MsaT043961.1:CDS
GGTGATGTTATAATCTACAC+TGG 0.627079 8:-53855943 MsaT043961.1:CDS
ATAATCTACACTGGCCATGG+AGG 0.628518 8:-53855934 MsaT043961.1:CDS
TTCGTGGGATGAAGTATGAA+GGG 0.630187 8:-53855810 MsaT043961.1:CDS
GGGTAGTGCTGTTCTCAAAG+AGG 0.632731 8:-53855653 MsaT043961.1:CDS
ACCAGTGTGATTGTTTCGGG+TGG 0.634390 8:-53855994 MsaT043961.1:CDS
TCGAGGTTAGGGTTATTCGT+GGG 0.635248 8:-53855825 MsaT043961.1:CDS
ATTGCATATAGCATAACATG+AGG 0.637941 8:+53854890 None:intergenic
TCCAGAGAATAATCTAAAGG+AGG 0.638234 8:+53855013 None:intergenic
ATGATGGACGCACATAATCA+GGG 0.640525 8:+53855047 None:intergenic
TGAGGAGAGGAGGTTGACTG+AGG 0.642162 8:-53856358 MsaT043961.1:CDS
ATACTCGCAGATAAAGGCTC+CGG 0.644164 8:+53855189 None:intergenic
CGTGGTCGTAATCGGATACG+TGG 0.647223 8:-53856222 MsaT043961.1:CDS
ACACGATTCCGACAATTGGG+AGG 0.651351 8:+53855298 None:intergenic
TGTGTTGGCGAGTGTTGAGG+AGG 0.651996 8:-53856448 MsaT043961.1:CDS
GATGATATGGAATTGGATGA+CGG 0.654438 8:-53855964 MsaT043961.1:CDS
GAGTATGCATTATGGAATCG+AGG 0.658351 8:-53855842 MsaT043961.1:CDS
GGCAGGAGAAGAATTCCTCA+AGG 0.664502 8:-53855912 MsaT043961.1:CDS
GGAATCGTGTTGCACAAAAG+GGG 0.665750 8:-53855285 MsaT043961.1:CDS
GTGATGTGATGAGGAAGACT+AGG 0.666120 8:-53856491 MsaT043961.1:CDS
TGTGATCAGAAATTGGTGGG+CGG 0.670938 8:-53855883 MsaT043961.1:CDS
GATGTTGGCTGCTGAAGTTG+CGG 0.672496 8:-53856331 MsaT043961.1:CDS
TCTCAACCCTCCTAATTTGG+GGG 0.674105 8:-53856676 MsaT043961.1:CDS
ACCACCCGAAACAATCACAC+TGG 0.678969 8:+53855993 None:intergenic
AATTTGTGATCAGAAATTGG+TGG 0.678995 8:-53855887 MsaT043961.1:CDS
TGTTCCGGAGAAACAAGAGA+CGG 0.689119 8:-53856601 MsaT043961.1:CDS
TTGCATATAGCATAACATGA+GGG 0.689576 8:+53854891 None:intergenic
ATAAGCTATGGAGAATTGAG+GGG 0.697353 8:-53855687 MsaT043961.1:CDS
TCGAATGGAAGAAGGCGTCG+TGG 0.704784 8:-53856240 MsaT043961.1:CDS
TAATGAGGAAGCGTGAGTTG+TGG 0.705916 8:-53856179 MsaT043961.1:CDS
GTCCAACTATCCTCTTATCG+CGG 0.711768 8:+53856151 None:intergenic
TCTAAGCTGAGTTCTCTCAG+AGG 0.749448 8:+53854854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 53854805 53856775 53854805 ID=MsaG043961
Chr8 mRNA 53854805 53856775 53854805 ID=MsaT043961.1;Parent=MsaG043961
Chr8 exon 53854805 53856775 53854805 ID=MsaT043961.1.exon1;Parent=MsaT043961.1
Chr8 CDS 53854805 53856775 53854805 ID=cds.MsaT043961.1;Parent=MsaT043961.1
Gene Sequence

>MsaT043961.1

ATGGGAATCGACGATTCCAACAATGCTTCTGCTGTTTCAATGGAGAATTTGGTTAATGGTTGTAACCCTGACCTAAATCTCAACCCTCCTAATTTGGGGGTGGATTCTATTGATTCTGAGCCGTGTGAGGGAAATGATCGAGCAATTGTTGTTGTTCCGGAGAAACAAGAGACGGCAGTTGCGGTGGTTAGGAAGAAAGCAAGCCAGGTGATGGAGCTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCATTTTCGTGATGTGATGAGGAAGACTAGGATGATTTATGATTCGCTTCGTGTGTTGGCGAGTGTTGAGGAGGAGAAGAGAATGGCTGATGAGAGGAAATTGGCTGCTGAAGTTGCTGTGGAGGAAGAGAGGAGATTGGCTGAGGAGAGGAGGTTGACTGAGGAGAGGATGTTGGCTGCTGAAGTTGCGGTTATGGAGGCAGAAATGAGAGTTGGTGAGGAGAGTAGGGTGGCGGCTGCGGGGTTGGAAGGGGAGATGTCGAATGGAAGAAGGCGTCGTGGTCGTAATCGGATACGTGGTGATATGAGGGCTGCGGCGTTAATGAGGAAGCGTGAGTTGTGGCTGTACCGCGATAAGAGGATAGTTGGACCGATACCTGGAGTTTATGTTGGTGATGTGTTTTTGTTTAGGATGGAATTGTGTGTAATTGGTTTGCATATGCAGATACAAGCTGGCATTGATTACCTACCTAAAAGTAGGTGCTCAAATGGTGAGCCAATTGCTACCAGTGTGATTGTTTCGGGTGGTTATGAGGATGATATGGAATTGGATGACGGTGATGTTATAATCTACACTGGCCATGGAGGGCAGGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGTGGGCGGAAACCTTGCTTTGGAAAGGAGTATGCATTATGGAATCGAGGTTAGGGTTATTCGTGGGATGAAGTATGAAGGGAGCGCGTCCGGTTCTGGTAAGGTTTATGTTTATGATGGATTGTATAGGATTGTTGATTGTTGGTTTGATGTAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGCTATGGAGAATTGAGGGGCAGGCTAAGATGGGTAGTGCTGTTCTCAAAGAGGCTAGGGAGATTAAGAAAATTGGGTTGGATGTTAAGCCTATGTATTGTCTTTCTGTTGATATTTCACACAAGAGGGAGAATGTTCCTATTAGGCTATTTAATGATATAGATGACAATCAAGATCCTCTTTATTATGAGTATCTTCCGAAAACTACTTTTCCGCAGTTTGTGTTTCATCAGAGTGGGAATGCTACTGGCTGTGAATGTGTGGAGGCTTGTACTGATGGCTGCTTTTGCTCTATGAAGAATGGAGGAGAGTTTCCCTATAATCTTCAGGGTTTGCTTGTGAGAGGGAGGCCTTTGATTTTTGAATGTGGCCCTTTTTGCTCCTGTCCTCCCAATTGTCGGAATCGTGTTGCACAAAAGGGGATCAGGCATAGGTTGGAAGTGTTTAGGTCTGCGCAGACTGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCCGGAGCCTTTATCTGCGAGTATACGGGTGTAATTTTAACAAGGGAGCAAGCTCAAATTTTAACAATGAATGGTGACTCCTTAATATATCCTAATCGGTTTTCAGATAGATGGGCAGAATGGGGGGATTTGTCTCAGATATACCCTGATTATGTGCGTCCATCATATCCCTCAATTCCTCCTTTAGATTATTCTCTGGATGTATCAACAATGAGAAATGTTGCTTGTTACATGAGCCATAGTTCGAGTCCGAATGTGTTTGTTCAGTATGTTCTGTTTGATCACAACAATTTAATGGTCCCTCATGTTATGCTATATGCAATGGAGAACATCCCTCCTCTGAGAGAACTCAGCTTAGATTATGGCATCGCAGACGAGTGGACAGGCAAGCTGTCTATATGTATGTAA
Protein sequence

>MsaT043961.1

MGIDDSNNASAVSMENLVNGCNPDLNLNPPNLGVDSIDSEPCEGNDRAIVVVPEKQETAVAVVRKKASQVMELVRVSDLSMKDQMHFRDVMRKTRMIYDSLRVLASVEEEKRMADERKLAAEVAVEEERRLAEERRLTEERMLAAEVAVMEAEMRVGEESRVAAAGLEGEMSNGRRRRGRNRIRGDMRAAALMRKRELWLYRDKRIVGPIPGVYVGDVFLFRMELCVIGLHMQIQAGIDYLPKSRCSNGEPIATSVIVSGGYEDDMELDDGDVIIYTGHGGQEKNSSRQICDQKLVGGNLALERSMHYGIEVRVIRGMKYEGSASGSGKVYVYDGLYRIVDCWFDVGKSGFGVYKYKLWRIEGQAKMGSAVLKEAREIKKIGLDVKPMYCLSVDISHKRENVPIRLFNDIDDNQDPLYYEYLPKTTFPQFVFHQSGNATGCECVEACTDGCFCSMKNGGEFPYNLQGLLVRGRPLIFECGPFCSCPPNCRNRVAQKGIRHRLEVFRSAQTGWGVRSLDLIQAGAFICEYTGVILTREQAQILTMNGDSLIYPNRFSDRWAEWGDLSQIYPDYVRPSYPSIPPLDYSLDVSTMRNVACYMSHSSSPNVFVQYVLFDHNNLMVPHVMLYAMENIPPLRELSLDYGIADEWTGKLSICM*