AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG043997

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043997 MtrunA17_Chr8g0369301 85.640 773 79 3 1 765 1 749 0.0 1308
MsaG043997 MtrunA17_Chr8g0368901 74.264 781 173 8 1 765 1 769 0.0 1148
MsaG043997 MtrunA17_Chr8g0368911 64.713 785 223 9 1 761 4 758 0.0 1012
MsaG043997 MtrunA17_Chr1g0163051 62.268 538 188 6 229 764 134 658 0.0 693
MsaG043997 MtrunA17_Chr1g0163041 61.702 470 177 3 288 756 101 568 0.0 610
MsaG043997 MtrunA17_Chr6g0467531 57.204 465 176 8 298 753 114 564 0.0 535
MsaG043997 MtrunA17_Chr6g0467381 56.344 465 180 8 298 753 114 564 0.0 530
MsaG043997 MtrunA17_Chr6g0467421 56.344 465 180 8 298 753 114 564 0.0 529
MsaG043997 MtrunA17_Chr6g0467401 55.914 465 182 8 298 753 114 564 7.04e-179 524
MsaG043997 MtrunA17_Chr3g0094881 50.784 510 224 8 267 764 144 638 6.85e-169 501
MsaG043997 MtrunA17_Chr6g0467411 51.078 464 185 8 298 753 53 482 5.85e-156 462
MsaG043997 MtrunA17_Chr2g0306961 44.956 565 201 13 194 753 4 463 1.34e-146 437
MsaG043997 MtrunA17_Chr7g0255181 41.408 483 243 16 296 765 239 694 1.84e-105 338
MsaG043997 MtrunA17_Chr5g0399801 40.000 460 253 8 296 751 618 1058 5.96e-105 346
MsaG043997 MtrunA17_Chr3g0080761 35.778 559 324 13 204 752 100 633 3.06e-103 331
MsaG043997 MtrunA17_Chr4g0003501 40.851 470 253 12 286 751 476 924 1.16e-102 337
MsaG043997 MtrunA17_Chr4g0003511 40.741 459 250 9 296 751 582 1021 4.97e-101 335
MsaG043997 MtrunA17_Chr8g0354821 39.958 473 251 13 296 753 204 658 1.12e-99 323
MsaG043997 MtrunA17_Chr6g0476111 38.669 481 256 14 296 752 199 664 3.03e-89 295
MsaG043997 MtrunA17_Chr7g0252211 38.174 482 258 13 296 752 261 727 6.59e-89 296
MsaG043997 MtrunA17_Chr6g0476121 38.046 481 259 13 296 752 148 613 2.05e-86 286
MsaG043997 MtrunA17_Chr7g0232811 30.502 518 232 17 280 751 73 508 6.32e-56 201
MsaG043997 MtrunA17_Chr1g0170651 32.766 470 265 15 298 751 86 520 3.58e-50 185
MsaG043997 MtrunA17_Chr7g0232981 30.087 462 234 14 296 751 107 485 3.02e-49 181
MsaG043997 MtrunA17_Chr7g0232841 29.583 480 224 17 295 751 109 497 2.08e-45 171
MsaG043997 MtrunA17_Chr8g0357391 55.882 136 54 4 184 316 2 134 5.32e-36 134
MsaG043997 MtrunA17_Chr5g0403241 31.492 181 112 3 568 747 1310 1479 4.57e-20 95.9
MsaG043997 MtrunA17_Chr8g0373821 28.214 280 154 9 503 751 252 515 8.07e-20 94.0
MsaG043997 MtrunA17_Chr4g0075321 29.630 189 111 3 569 751 868 1040 1.17e-19 94.4
MsaG043997 MtrunA17_Chr1g0183321 23.529 306 164 9 503 753 430 720 5.20e-18 89.0
MsaG043997 MtrunA17_Chr7g0232861 36.601 153 78 7 296 445 106 242 4.02e-17 81.6
MsaG043997 MtrunA17_Chr5g0448331 36.076 158 87 4 321 473 267 415 2.02e-16 83.6
MsaG043997 MtrunA17_Chr8g0389931 36.076 158 87 4 321 473 261 409 3.53e-16 82.8
MsaG043997 MtrunA17_Chr3g0143561 33.333 153 88 4 321 473 187 325 5.15e-13 71.6
MsaG043997 MtrunA17_Chr7g0232761 28.287 251 130 10 226 450 38 264 8.17e-13 69.7
MsaG043997 MtrunA17_Chr6g0460921 27.184 206 123 7 549 752 217 397 3.73e-11 65.9
MsaG043997 MtrunA17_Chr7g0232751 46.552 58 31 0 694 751 31 88 3.80e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG043997 AT4G13460.1 61.031 485 182 5 282 764 170 649 0.0 630
MsaG043997 AT4G13460.2 61.031 485 182 5 282 764 170 649 0.0 630
MsaG043997 AT2G33290.1 58.264 484 190 5 282 764 178 650 0.0 602
MsaG043997 AT2G33290.3 58.264 484 190 5 282 764 178 650 0.0 602
MsaG043997 AT2G33290.4 58.264 484 190 5 282 764 178 650 0.0 602
MsaG043997 AT2G33290.2 58.264 484 190 5 282 764 178 650 0.0 602
MsaG043997 AT5G04940.2 39.959 483 260 15 279 753 180 640 1.77e-97 317
MsaG043997 AT5G04940.1 39.959 483 260 15 279 753 180 640 1.77e-97 317
MsaG043997 AT2G22740.2 37.029 478 274 8 285 751 298 759 1.43e-93 310
MsaG043997 AT2G22740.1 37.029 478 274 8 285 751 298 759 1.43e-93 310
MsaG043997 AT1G73100.1 35.921 554 313 16 220 753 108 639 1.86e-92 303
MsaG043997 AT2G35160.5 34.857 525 291 13 236 751 245 727 3.74e-89 297
MsaG043997 AT2G35160.2 35.048 525 290 14 236 751 281 763 9.53e-89 296
MsaG043997 AT2G35160.3 35.048 525 290 14 236 751 281 763 9.53e-89 296
MsaG043997 AT2G35160.1 35.048 525 290 14 236 751 281 763 9.53e-89 296
MsaG043997 AT2G35160.4 35.048 525 290 14 236 751 281 763 9.53e-89 296
MsaG043997 AT5G13960.1 37.551 490 249 17 296 752 129 594 1.28e-85 284
MsaG043997 AT5G13960.2 38.055 473 237 16 313 752 11 460 7.23e-85 278
MsaG043997 AT2G24740.1 35.882 510 286 17 253 753 247 724 3.70e-72 251
MsaG043997 AT1G17770.1 31.507 511 317 15 258 753 169 661 4.76e-72 249
MsaG043997 AT5G47150.1 40.449 178 97 4 296 473 156 324 5.07e-28 116
MsaG043997 AT5G47160.1 40.351 171 92 5 300 469 246 407 1.16e-24 107
MsaG043997 AT5G47160.3 40.351 171 92 5 300 469 246 407 1.16e-24 107
MsaG043997 AT5G47160.2 40.351 171 92 5 300 469 246 407 1.16e-24 107
MsaG043997 AT2G23740.3 30.457 197 110 5 570 756 1184 1363 5.25e-21 99.4
MsaG043997 AT2G23740.6 30.457 197 110 5 570 756 1184 1363 5.25e-21 99.4
MsaG043997 AT2G23740.2 30.457 197 110 5 570 756 1184 1363 5.25e-21 99.4
MsaG043997 AT2G23740.1 30.457 197 110 5 570 756 1177 1356 5.66e-21 99.4
MsaG043997 AT2G05900.1 37.931 174 100 6 358 531 26 191 5.64e-19 89.0
MsaG043997 AT3G03750.2 28.070 285 145 11 501 752 66 323 2.82e-17 84.7
MsaG043997 AT1G57820.1 36.047 172 86 6 317 473 264 426 6.82e-17 85.5
MsaG043997 AT1G57820.3 36.047 172 86 6 317 473 264 426 6.82e-17 85.5
MsaG043997 AT1G57820.2 35.503 169 88 6 317 473 264 423 8.67e-16 82.0
MsaG043997 AT5G39550.1 34.302 172 89 6 317 473 249 411 1.02e-15 81.6
MsaG043997 AT2G23740.4 31.757 148 77 5 570 710 1184 1314 5.18e-15 80.1
MsaG043997 AT2G23740.5 31.757 148 77 5 570 710 1184 1314 5.18e-15 80.1
MsaG043997 AT1G66040.1 31.977 172 93 5 317 473 249 411 5.83e-15 79.3
MsaG043997 AT1G66050.1 31.977 172 93 5 317 473 249 411 6.66e-15 79.0
MsaG043997 AT1G57800.2 33.544 158 81 6 317 459 276 424 2.20e-11 67.8

Find 232 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAATCTCAACCTTAATT+TGG 0.115237 8:+54425634 MsaT043997.1:CDS
GCTAGGGATATTAAGAAAAT+TGG 0.127502 8:+54426670 MsaT043997.1:CDS
CCAAATTAAGGTTGAGATTT+AGG 0.131281 8:-54425634 None:intergenic
GCTACTAGTGTAATTGTTTC+AGG 0.143034 8:+54426304 MsaT043997.1:CDS
CCCCATTCTACCCATCTATT+TGG 0.166114 8:-54427213 None:intergenic
CTATTGATATTTCAAATAAT+AGG 0.206413 8:+54426722 MsaT043997.1:CDS
AGAAATTGTTAAAAGGTTTC+GGG 0.218771 8:-54425430 None:intergenic
GCAAATTTGTGATCAGAAAT+TGG 0.219259 8:+54426411 MsaT043997.1:CDS
ACATACTGAATGAATATATT+TGG 0.228005 8:-54427360 None:intergenic
GGTGGATATGGTGGTGATTT+GGG 0.244564 8:-54425375 None:intergenic
TAGGTCCTTGGACCTTATTC+AGG 0.261644 8:+54427089 MsaT043997.1:CDS
AAGAAATTGTTAAAAGGTTT+CGG 0.264339 8:-54425431 None:intergenic
AAGCAATCCATGTAGATTAT+AGG 0.265044 8:-54426932 None:intergenic
AAACTCGACTCTTGTTTCTT+AGG 0.266801 8:-54425704 None:intergenic
CTAAATCTCAACCTTAATTT+GGG 0.267648 8:+54425635 MsaT043997.1:CDS
GGTGAGAGCGGTGTGATTTA+AGG 0.267982 8:-54425348 None:intergenic
CGGTGGATATGGTGGTGATT+TGG 0.269392 8:-54425376 None:intergenic
TCAGATTCGGGAGAGAAATT+AGG 0.279456 8:-54425479 None:intergenic
TGATTTAAGGTTGAAATTCT+AGG 0.282052 8:-54425335 None:intergenic
TGAGAGTGGGTGAGGAGAAT+AGG 0.282380 8:+54426008 MsaT043997.1:CDS
TAGGTTGTTGTTGGGAATCT+TGG 0.291847 8:-54425460 None:intergenic
GAGAGTGGGTGAGGAGAATA+GGG 0.304281 8:+54426009 MsaT043997.1:CDS
AATTGGTTGTTGAAGTTGAT+AGG 0.309699 8:+54425900 MsaT043997.1:CDS
TATTGATATTTCAAATAATA+GGG 0.311494 8:+54426723 MsaT043997.1:CDS
TGATCATCGGTTTCAGATTC+GGG 0.313526 8:-54425491 None:intergenic
GCAACACGGTTTCGACAATT+AGG 0.314745 8:-54427006 None:intergenic
GATATAGGAAAGTCTGGTTT+TGG 0.315495 8:+54426580 MsaT043997.1:CDS
GAAGGGGTTTCAAGGGTTAA+TGG 0.316999 8:-54425233 None:intergenic
TTGATCATCGGTTTCAGATT+CGG 0.324605 8:-54425492 None:intergenic
GTCGAGTTTGATGGCGGATA+TGG 0.325154 8:+54425718 MsaT043997.1:CDS
AATATAGCTTGGAAGTGTTT+AGG 0.327076 8:+54427043 MsaT043997.1:CDS
AATGAATGAGGAGAAGAAAT+TGG 0.327732 8:+54425883 MsaT043997.1:CDS
GACGCGAAGAAATTGTTAAA+AGG 0.332988 8:-54425437 None:intergenic
ATATATTTGGACTCGAACTA+TGG 0.334818 8:-54427347 None:intergenic
ATCTTGCTCGCCCACTCGTC+CGG 0.340319 8:-54427480 None:intergenic
AGAGAACTCTGCATAGATTA+TGG 0.345054 8:+54427453 MsaT043997.1:CDS
AAGGGGTTTCAAGGGTTAAT+GGG 0.345399 8:-54425232 None:intergenic
GGGAGACAATTAATGGAATA+AGG 0.348461 8:+54426053 MsaT043997.1:CDS
AGCAATCCATGTAGATTATA+GGG 0.350043 8:-54426931 None:intergenic
ATATCATTAAATAGCCTAAC+TGG 0.350719 8:-54426754 None:intergenic
GTTAGTTGCTTGTTTGATAT+AGG 0.351027 8:+54426565 MsaT043997.1:CDS
CACAGACAGGTTGGGGAGTT+AGG 0.355710 8:+54427070 MsaT043997.1:CDS
ATTGGATGAGGAGAAGATAT+TGG 0.356889 8:+54425955 MsaT043997.1:CDS
TTGTTAAAAGGTTTCGGGTT+TGG 0.358135 8:-54425425 None:intergenic
CTAGTACATTTCCAAATAGA+TGG 0.359463 8:+54427202 MsaT043997.1:CDS
GAAGGGAGTGCATCTACTTC+TGG 0.362995 8:+54426508 MsaT043997.1:CDS
TAAAGGATCCAGCCTGAATA+AGG 0.368631 8:-54427101 None:intergenic
ATTGTCTGAGGAAAGGAAAT+TGG 0.371576 8:+54425937 MsaT043997.1:CDS
TGTGGTAGAATTTGGTGTAT+AGG 0.373147 8:-54425520 None:intergenic
AAGTGATGTTCCGGTGGATA+TGG 0.376573 8:-54425387 None:intergenic
TAGAATTTGGTGTATAGGTT+GGG 0.381830 8:-54425515 None:intergenic
TATGGTGGTGATTTGGGGTG+GGG 0.386266 8:-54425369 None:intergenic
CGTAGTATCAAGCACATTCA+TGG 0.391102 8:+54426079 MsaT043997.1:CDS
AAGTAGAAGAAGGGGTTTCA+AGG 0.393513 8:-54425241 None:intergenic
CTAGGGATATTAAGAAAATT+GGG 0.394850 8:+54426671 MsaT043997.1:CDS
TCGAAACCGTGTTGCACAAA+AGG 0.395320 8:+54427014 MsaT043997.1:CDS
TTTGATGGCGGATATGGTTA+TGG 0.396895 8:+54425724 MsaT043997.1:CDS
TGTTGTTTCATTGGAGAATA+CGG 0.399431 8:+54425592 MsaT043997.1:CDS
TCCAAATAGATGGGTAGAAT+GGG 0.401426 8:+54427212 MsaT043997.1:CDS
GAGAGAAATTAGGTTGTTGT+TGG 0.401584 8:-54425469 None:intergenic
TGCACTATGGGATTGAGGTT+AGG 0.405470 8:+54426464 MsaT043997.1:CDS
TCAAAGAGTATGTCTTATAA+TGG 0.405858 8:+54426271 MsaT043997.1:CDS
GTAGAATTTGGTGTATAGGT+TGG 0.407762 8:-54425516 None:intergenic
GATATGGTGGTGATTTGGGG+TGG 0.408728 8:-54425371 None:intergenic
ACCAGTATACTCGCATATAA+AGG 0.412252 8:-54427118 None:intergenic
GGGGTGGATTCTATAATTGA+TGG 0.415475 8:+54425656 MsaT043997.1:CDS
GCACTATGGGATTGAGGTTA+GGG 0.416487 8:+54426465 MsaT043997.1:CDS
AACAAAATCATTGTTGGAAT+CGG 0.418151 8:-54425573 None:intergenic
TTCCAAATAGATGGGTAGAA+TGG 0.421060 8:+54427211 MsaT043997.1:CDS
TTGTTTGATATAGGAAAGTC+TGG 0.421956 8:+54426574 MsaT043997.1:CDS
TAGGTCTACACAGACAGGTT+GGG 0.422135 8:+54427062 MsaT043997.1:CDS
TGAAACAACAAAATCATTGT+TGG 0.424236 8:-54425579 None:intergenic
GGCAGAGATGAGAGTGGGTG+AGG 0.425042 8:+54426000 MsaT043997.1:CDS
AGGTTGTTGTTGGGAATCTT+GGG 0.427326 8:-54425459 None:intergenic
ATATGGTGGTGATTTGGGGT+GGG 0.427929 8:-54425370 None:intergenic
CATCTATTTGGAAATGTACT+AGG 0.428903 8:-54427201 None:intergenic
AGCACATTCATGGTTATATG+AGG 0.429812 8:+54426089 MsaT043997.1:CDS
TGCCGTTTGAAGTGATGTTC+CGG 0.434923 8:-54425396 None:intergenic
TATAAGTTATTGAGAATTGA+GGG 0.436671 8:+54426613 MsaT043997.1:CDS
TCTTTATTATGAGTATCTTC+CGG 0.438277 8:+54426795 MsaT043997.1:CDS
TTAGGAGGACATGAGCAAAA+AGG 0.439015 8:-54426988 None:intergenic
AGTAGAAGAAGGGGTTTCAA+GGG 0.441073 8:-54425240 None:intergenic
GAGAAATAGAAGTAGAAGAA+GGG 0.442706 8:-54425250 None:intergenic
ATTGGTTGTTGAAGTTGATA+GGG 0.446123 8:+54425901 MsaT043997.1:CDS
TCCTTGGACCTTATTCAGGC+TGG 0.446886 8:+54427093 MsaT043997.1:CDS
GTGTCGGCTACCAGCGTGGA+GGG 0.446897 8:+54426031 MsaT043997.1:CDS
CGAAACCGTGTTGCACAAAA+GGG 0.448108 8:+54427015 MsaT043997.1:CDS
AATTGAGGGGCAGGCGAAGA+TGG 0.448823 8:+54426627 MsaT043997.1:CDS
AGGAGGGAATTGTGTGTGAC+TGG 0.450008 8:+54426211 MsaT043997.1:CDS
GTCGGCTACCAGCGTGGAGG+GGG 0.454564 8:+54426033 MsaT043997.1:CDS
ACCTAGTGGTTGTGAATGTA+AGG 0.454638 8:+54426858 MsaT043997.1:CDS
TGAGAAATAGAAGTAGAAGA+AGG 0.457135 8:-54425251 None:intergenic
GGAATAGGAACATGAGAAGA+AGG 0.457689 8:-54425275 None:intergenic
GAAACAAGAGTCGAGTTTGA+TGG 0.459669 8:+54425709 MsaT043997.1:CDS
AGAGAAATTAGGTTGTTGTT+GGG 0.464968 8:-54425468 None:intergenic
TCCTTTATATGCGAGTATAC+TGG 0.466074 8:+54427117 MsaT043997.1:CDS
TTGATGAGAAATACTAAGAT+CGG 0.467844 8:-54425309 None:intergenic
GATTTAAGGTTGAAATTCTA+GGG 0.470586 8:-54425334 None:intergenic
TTGGAAAGGAGTATGCACTA+TGG 0.475190 8:+54426451 MsaT043997.1:CDS
TGTCGGCTACCAGCGTGGAG+GGG 0.476100 8:+54426032 MsaT043997.1:CDS
ACATCACCAACATAAATTCC+AGG 0.479414 8:-54426178 None:intergenic
TAAGGTTGAGATTTAGGTCC+GGG 0.480083 8:-54425628 None:intergenic
TGATGAGAAATACTAAGATC+GGG 0.480139 8:-54425308 None:intergenic
CACCGGAACATCACTTCAAA+CGG 0.481144 8:+54425394 MsaT043997.1:CDS
GGTTTGTGTTTCATCAGAGT+GGG 0.481554 8:+54426833 MsaT043997.1:CDS
GGATATTAAGAAAATTGGGT+TGG 0.483689 8:+54426675 MsaT043997.1:CDS
AGGATAGTTGGACCGATACC+TGG 0.485997 8:+54426160 MsaT043997.1:CDS
TTCGCTTCGCGTGAGAATTG+AGG 0.488587 8:+54425850 MsaT043997.1:CDS
TCTTGCTCGCCCACTCGTCC+GGG 0.490402 8:-54427479 None:intergenic
GGTTATGGAGAAGGAAAGCC+AGG 0.494082 8:+54425739 MsaT043997.1:CDS
AATTGTTTCAGGTGGTTATG+AGG 0.495448 8:+54426315 MsaT043997.1:CDS
TAATCTAAAGGAGGTATTGA+CGG 0.498715 8:-54427279 None:intergenic
ATAGGGAAAATGTTCCAGTT+AGG 0.499113 8:+54426740 MsaT043997.1:CDS
TATCGTGATCAAAGGATAGT+TGG 0.501111 8:+54426148 MsaT043997.1:CDS
GTATAAGTTATTGAGAATTG+AGG 0.501728 8:+54426612 MsaT043997.1:CDS
CACTGTTCTCAAAGATGCTA+GGG 0.502270 8:+54426654 MsaT043997.1:CDS
CCCTCACAAGTTCCATCACC+TGG 0.503770 8:-54425757 None:intergenic
TCTATGCAGAGTTCTCTCAT+AGG 0.506224 8:-54427447 None:intergenic
AAAGGGGCTCAAATATAGCT+TGG 0.511664 8:+54427032 MsaT043997.1:CDS
AGGTCTACACAGACAGGTTG+GGG 0.513098 8:+54427063 MsaT043997.1:CDS
GTATAGGTTGGGTTGATCAT+CGG 0.513117 8:-54425504 None:intergenic
GATTTGGGGTGGGGTGAGAG+CGG 0.514925 8:-54425360 None:intergenic
ACTCGAACTATGGCTCATAT+AGG 0.515301 8:-54427337 None:intergenic
TTAAGGTTGAGATTTAGGTC+CGG 0.515596 8:-54425629 None:intergenic
GAGTGCATCTACTTCTGGTA+AGG 0.518621 8:+54426513 MsaT043997.1:CDS
TCAATAGAGATACAACTCAT+TGG 0.521177 8:-54426706 None:intergenic
GGCGGATATGGTTATGGAGA+AGG 0.522194 8:+54425730 MsaT043997.1:CDS
GAGTTTCCCTATAATCTACA+TGG 0.522663 8:+54426925 MsaT043997.1:CDS
GCACTGTTCTCAAAGATGCT+AGG 0.522783 8:+54426653 MsaT043997.1:CDS
TGCACTTTCGTCATGAGATA+AGG 0.523361 8:+54425807 MsaT043997.1:CDS
GAATCGGCGATTTCGAGAAA+AGG 0.523869 8:-54425557 None:intergenic
GCATACTCCTTTCCAATGCA+AGG 0.525232 8:-54426444 None:intergenic
TCGTGTCATGTTATATGCAA+TGG 0.526750 8:+54427413 MsaT043997.1:CDS
GGAGGTATTGACGGATAAGA+TGG 0.528649 8:-54427270 None:intergenic
TAATGAAGGAGCGTGGGCTA+TGG 0.529339 8:+54426122 MsaT043997.1:CDS
AGAAATAGAAGTAGAAGAAG+GGG 0.530641 8:-54425249 None:intergenic
AGAAGAGATTGTCTGAGGAA+AGG 0.532258 8:+54425930 MsaT043997.1:CDS
ATTGAGGTTAGGGTTATTCG+TGG 0.532380 8:+54426475 MsaT043997.1:CDS
GGAGAAGGAAAGCCAGGTGA+TGG 0.532924 8:+54425745 MsaT043997.1:CDS
TGGTTTGTGTTTCATCAGAG+TGG 0.533225 8:+54426832 MsaT043997.1:CDS
ATTATAATCTACACTGGCCA+TGG 0.534081 8:+54426364 MsaT043997.1:CDS
GTTATTGAGAATTGAGGGGC+AGG 0.539794 8:+54426618 MsaT043997.1:CDS
ACAATTACACTAGTAGCAAT+CGG 0.540891 8:-54426298 None:intergenic
ATTCGTGGGATGAAGTATGA+AGG 0.541112 8:+54426490 MsaT043997.1:CDS
TGGAAAGGAGTATGCACTAT+GGG 0.541585 8:+54426452 MsaT043997.1:CDS
TACTGGTGTTGCTATGACTA+GGG 0.544129 8:+54427134 MsaT043997.1:CDS
AATTCTTCTCTCGCCCTCCA+TGG 0.544307 8:-54426381 None:intergenic
GGTTGTTGAAGTTGATAGGG+AGG 0.545333 8:+54425904 MsaT043997.1:CDS
ACATCCAGAAAATAATCTAA+AGG 0.546485 8:-54427291 None:intergenic
CTGATCACAAATTTGCCTTG+AGG 0.547153 8:-54426404 None:intergenic
ATTGAGGGGCAGGCGAAGAT+GGG 0.548419 8:+54426628 MsaT043997.1:CDS
TTCGTGGGATGAAGTATGAA+GGG 0.548806 8:+54426491 MsaT043997.1:CDS
GAGTATGCACTATGGGATTG+AGG 0.552471 8:+54426459 MsaT043997.1:CDS
CCAGGTGATGGAACTTGTGA+GGG 0.555665 8:+54425757 MsaT043997.1:CDS
ATGCAGAGTTCTCTCATAGG+AGG 0.558353 8:-54427444 None:intergenic
GGAGGGTGGAAACCTTGCAT+TGG 0.558530 8:+54426432 MsaT043997.1:CDS
GGTGTCGGCTACCAGCGTGG+AGG 0.558545 8:+54426030 MsaT043997.1:CDS
CTCTGATGAAACACAAACCA+CGG 0.558774 8:-54426829 None:intergenic
GGAAGATACTCATAATAAAG+AGG 0.560347 8:-54426793 None:intergenic
GGTAAGGTTTATGTTTACGA+TGG 0.560574 8:+54426529 MsaT043997.1:CDS
GTTAATGGGAAAGGATCCAT+TGG 0.561622 8:-54425218 None:intergenic
AATCGGCGATTTCGAGAAAA+GGG 0.562107 8:-54425556 None:intergenic
TTGAGGTTAGGGTTATTCGT+GGG 0.565646 8:+54426476 MsaT043997.1:CDS
TGAGGAAAGGAAATTGGATG+AGG 0.567529 8:+54425943 MsaT043997.1:CDS
GGAGGACAAGAGAATGAATG+AGG 0.571585 8:+54425871 MsaT043997.1:CDS
GGAAGAGAAGAGATTGTCTG+AGG 0.572868 8:+54425925 MsaT043997.1:CDS
CCGATACCTGGAATTTATGT+TGG 0.577931 8:+54426172 MsaT043997.1:CDS
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8:+54425778 MsaT043997.1:CDS
GTGGAAACCTTGCATTGGAA+AGG 0.581268 8:+54426437 MsaT043997.1:CDS
GTTTCAAGGGTTAATGGGAA+AGG 0.581604 8:-54425227 None:intergenic
GTGGATATGGTGGTGATTTG+GGG 0.583200 8:-54425374 None:intergenic
AACCACGGGAAAGTAGAATC+CGG 0.583577 8:-54426814 None:intergenic
CGACATTAATGAAGGAGCGT+GGG 0.584881 8:+54426116 MsaT043997.1:CDS
TAGTACATTTCCAAATAGAT+GGG 0.585798 8:+54427203 MsaT043997.1:CDS
ATACTGGTGTTGCTATGACT+AGG 0.586035 8:+54427133 MsaT043997.1:CDS
GAGGGCTGCGACATTAATGA+AGG 0.586703 8:+54426108 MsaT043997.1:CDS
CTGCATAGATTATGGCATCC+CGG 0.587677 8:+54427461 MsaT043997.1:CDS
GGATGCAGAAGTTGCAGTTG+TGG 0.588062 8:+54425976 MsaT043997.1:CDS
CCAACATAAATTCCAGGTAT+CGG 0.588388 8:-54426172 None:intergenic
TAAATCTCAACCTTAATTTG+GGG 0.590545 8:+54425636 MsaT043997.1:CDS
GTGTTTAGGTCTACACAGAC+AGG 0.591190 8:+54427057 MsaT043997.1:CDS
TATGGTTGTATCGTGATCAA+AGG 0.592056 8:+54426140 MsaT043997.1:CDS
TGTGATCAGAAATTGGAGGG+TGG 0.592511 8:+54426418 MsaT043997.1:CDS
GCCAGGTGATGGAACTTGTG+AGG 0.598287 8:+54425756 MsaT043997.1:CDS
TGCAGAGTTCTCTCATAGGA+GGG 0.599457 8:-54427443 None:intergenic
TAATCTACACTGGCCATGGA+GGG 0.600090 8:+54426368 MsaT043997.1:CDS
ACACGGTTTCGACAATTAGG+AGG 0.600465 8:-54427003 None:intergenic
ACTAGTGTAATTGTTTCAGG+TGG 0.601921 8:+54426307 MsaT043997.1:CDS
AGGTTGGGGAGTTAGGTCCT+TGG 0.602845 8:+54427077 MsaT043997.1:CDS
AGGTGGTTATGAGGATGACA+TGG 0.604358 8:+54426324 MsaT043997.1:CDS
TTCCGGATTCTACTTTCCCG+TGG 0.605395 8:+54426812 MsaT043997.1:CDS
GGTGATATTATAATCTACAC+TGG 0.606082 8:+54426358 MsaT043997.1:CDS
GTGGAGGCAGAGATGAGAGT+GGG 0.608465 8:+54425995 MsaT043997.1:CDS
GCGACATTAATGAAGGAGCG+TGG 0.612288 8:+54426115 MsaT043997.1:CDS
ATTATGGCATCCCGGACGAG+TGG 0.613316 8:+54427469 MsaT043997.1:CDS
TCTGATGAAACACAAACCAC+GGG 0.613935 8:-54426828 None:intergenic
ATTTGTGATCAGAAATTGGA+GGG 0.614502 8:+54426415 MsaT043997.1:CDS
CAAATCACCACCATATCCAC+CGG 0.614581 8:+54425377 MsaT043997.1:CDS
TTGCATATGCATATGCAAGC+TGG 0.615698 8:+54426235 MsaT043997.1:CDS
GCACATTCATGGTTATATGA+GGG 0.618630 8:+54426090 MsaT043997.1:CDS
GTTTGTGTTTCATCAGAGTG+GGG 0.619286 8:+54426834 MsaT043997.1:CDS
GGGTGAGGAGAATAGGGTGT+CGG 0.621767 8:+54426015 MsaT043997.1:CDS
TCCAGCCTGAATAAGGTCCA+AGG 0.622985 8:-54427094 None:intergenic
ATAAGTTATTGAGAATTGAG+GGG 0.623555 8:+54426614 MsaT043997.1:CDS
TTCGTCATGAGATAAGGAAG+AGG 0.623683 8:+54425813 MsaT043997.1:CDS
TTATGGCATCCCGGACGAGT+GGG 0.624731 8:+54427470 MsaT043997.1:CDS
ATAATCTACACTGGCCATGG+AGG 0.626398 8:+54426367 MsaT043997.1:CDS
TCCAGAAAATAATCTAAAGG+AGG 0.632885 8:-54427288 None:intergenic
TTAGGTCTACACAGACAGGT+TGG 0.633420 8:+54427061 MsaT043997.1:CDS
TGTAAGGAGGCTTGTACTGA+CGG 0.637592 8:+54426874 MsaT043997.1:CDS
TGCAGAAGTTGCAGTTGTGG+AGG 0.639345 8:+54425979 MsaT043997.1:CDS
TGATGTTCCGGTGGATATGG+TGG 0.640019 8:-54425384 None:intergenic
GATGAGAAATACTAAGATCG+GGG 0.640700 8:-54425307 None:intergenic
TACGGTAAATGTTTGTAACC+CGG 0.645041 8:+54425610 MsaT043997.1:CDS
GCTTCGCGTGAGAATTGAGG+AGG 0.645275 8:+54425853 MsaT043997.1:CDS
AAATCTCAACCTTAATTTGG+GGG 0.645557 8:+54425637 MsaT043997.1:CDS
CCAAATAGATGGGTAGAATG+GGG 0.657480 8:+54427213 MsaT043997.1:CDS
TCCTTACATTCACAACCACT+AGG 0.658950 8:-54426859 None:intergenic
CATCAGAGTGGGGAACCTAG+TGG 0.659288 8:+54426844 MsaT043997.1:CDS
GGCGAGAGAAGAATTCCTCA+AGG 0.664502 8:+54426389 MsaT043997.1:CDS
GAAACCGTGTTGCACAAAAG+GGG 0.665958 8:+54427016 MsaT043997.1:CDS
TAGGGTGTCGGCTACCAGCG+TGG 0.669330 8:+54426027 MsaT043997.1:CDS
ACAAGAGTCGAGTTTGATGG+CGG 0.673531 8:+54425712 MsaT043997.1:CDS
ATGAGAAATACTAAGATCGG+GGG 0.677362 8:-54425306 None:intergenic
TAGTGGTTGTGAATGTAAGG+AGG 0.682180 8:+54426861 MsaT043997.1:CDS
CGTTTGAAGTGATGTTCCGG+TGG 0.695613 8:-54425393 None:intergenic
AATTTGTGATCAGAAATTGG+AGG 0.699714 8:+54426414 MsaT043997.1:CDS
TGTGGAGGCAGAGATGAGAG+TGG 0.704475 8:+54425994 MsaT043997.1:CDS
GATGACATGGAATTAGACAA+TGG 0.725113 8:+54426337 MsaT043997.1:CDS
ATTGCATATAACATGACACG+AGG 0.728503 8:-54427411 None:intergenic
AAATGATCGAGCTATTGTTG+TGG 0.743063 8:+54425679 MsaT043997.1:CDS
TTGCATATAACATGACACGA+GGG 0.748835 8:-54427410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 54425221 54427518 54425221 ID=MsaG043997
Chr8 mRNA 54425221 54427518 54425221 ID=MsaT043997.1;Parent=MsaG043997
Chr8 exon 54425221 54427518 54425221 ID=MsaT043997.1.exon1;Parent=MsaT043997.1
Chr8 CDS 54425221 54427518 54425221 ID=cds.MsaT043997.1;Parent=MsaT043997.1
Gene Sequence

>MsaT043997.1

ATGGATCCTTTCCCATTAACCCTTGAAACCCCTTCTTCTACTTCTATTTCTCAACCTTCTTCTCATGTTCCTATTCCTACTATCACCCCCGATCTTAGTATTTCTCATCAAATCCCTAGAATTTCAACCTTAAATCACACCGCTCTCACCCCACCCCAAATCACCACCATATCCACCGGAACATCACTTCAAACGGCACTCATTCCAAACCCGAAACCTTTTAACAATTTCTTCGCGTCCCAAGATTCCCAACAACAACCTAATTTCTCTCCCGAATCTGAAACCGATGATCAACCCAACCTATACACCAAATTCTACCACACCTCAAAACTCTACCCTTTTCTCGAAATCGCCGATTCCAACAATGATTTTGTTGTTTCATTGGAGAATACGGTAAATGTTTGTAACCCGGACCTAAATCTCAACCTTAATTTGGGGGTGGATTCTATAATTGATGGAAATGATCGAGCTATTGTTGTGGCTCCTAAGAAACAAGAGTCGAGTTTGATGGCGGATATGGTTATGGAGAAGGAAAGCCAGGTGATGGAACTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCACTTTCGTCATGAGATAAGGAAGAGGCGAATGATTTATGATTCGCTTCGCGTGAGAATTGAGGAGGACAAGAGAATGAATGAGGAGAAGAAATTGGTTGTTGAAGTTGATAGGGAGGAAGAGAAGAGATTGTCTGAGGAAAGGAAATTGGATGAGGAGAAGATATTGGATGCAGAAGTTGCAGTTGTGGAGGCAGAGATGAGAGTGGGTGAGGAGAATAGGGTGTCGGCTACCAGCGTGGAGGGGGAGACAATTAATGGAATAAGGCGTCGTAGTATCAAGCACATTCATGGTTATATGAGGGCTGCGACATTAATGAAGGAGCGTGGGCTATGGTTGTATCGTGATCAAAGGATAGTTGGACCGATACCTGGAATTTATGTTGGTGATGTGTTTTTGTTTAGGAGGGAATTGTGTGTGACTGGTTTGCATATGCATATGCAAGCTGGTATTGATTATCTATCAAAGAGTATGTCTTATAATGGTGAACCGATTGCTACTAGTGTAATTGTTTCAGGTGGTTATGAGGATGACATGGAATTAGACAATGGTGATATTATAATCTACACTGGCCATGGAGGGCGAGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGAGGGTGGAAACCTTGCATTGGAAAGGAGTATGCACTATGGGATTGAGGTTAGGGTTATTCGTGGGATGAAGTATGAAGGGAGTGCATCTACTTCTGGTAAGGTTTATGTTTACGATGGATTATATAAGATTGTTAGTTGCTTGTTTGATATAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGTTATTGAGAATTGAGGGGCAGGCGAAGATGGGTAGCACTGTTCTCAAAGATGCTAGGGATATTAAGAAAATTGGGTTGGATTTCATGCCAATGAGTTGTATCTCTATTGATATTTCAAATAATAGGGAAAATGTTCCAGTTAGGCTATTTAATGATATAGATGTCAATCATGAACCTCTTTATTATGAGTATCTTCCGGATTCTACTTTCCCGTGGTTTGTGTTTCATCAGAGTGGGGAACCTAGTGGTTGTGAATGTAAGGAGGCTTGTACTGACGGTTGCTTTTGTGCTATGAAGAATGGAGGAGAGTTTCCCTATAATCTACATGGATTGCTTTTGAAAGGGAAACCTTTGATTTTTGAATGTGGGCCTTTTTGCTCATGTCCTCCTAATTGTCGAAACCGTGTTGCACAAAAGGGGCTCAAATATAGCTTGGAAGTGTTTAGGTCTACACAGACAGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCTGGATCCTTTATATGCGAGTATACTGGTGTTGCTATGACTAGGGAACAAGCTCAAATTTTAACAATGAATGGGGGCTCGTTGGTGTATCCTAGTACATTTCCAAATAGATGGGTAGAATGGGGTGATTTATCTCAGATATACACTGAATATGTGCGACCATCTTATCCGTCAATACCTCCTTTAGATTATTTTCTGGATGTGTCAACAATGAGAAATGTTGCATCCTATATGAGCCATAGTTCGAGTCCAAATATATTCATTCAGTATGTTCTTTATGATCACAACAATTTGTTGTTCCCTCGTGTCATGTTATATGCAATGGAGAACATCCCTCCTATGAGAGAACTCTGCATAGATTATGGCATCCCGGACGAGTGGGCGAGCAAGATGTCTAGATGTATGTAA
Protein sequence

>MsaT043997.1

MDPFPLTLETPSSTSISQPSSHVPIPTITPDLSISHQIPRISTLNHTALTPPQITTISTGTSLQTALIPNPKPFNNFFASQDSQQQPNFSPESETDDQPNLYTKFYHTSKLYPFLEIADSNNDFVVSLENTVNVCNPDLNLNLNLGVDSIIDGNDRAIVVAPKKQESSLMADMVMEKESQVMELVRVSDLSMKDQMHFRHEIRKRRMIYDSLRVRIEEDKRMNEEKKLVVEVDREEEKRLSEERKLDEEKILDAEVAVVEAEMRVGEENRVSATSVEGETINGIRRRSIKHIHGYMRAATLMKERGLWLYRDQRIVGPIPGIYVGDVFLFRRELCVTGLHMHMQAGIDYLSKSMSYNGEPIATSVIVSGGYEDDMELDNGDIIIYTGHGGREKNSSRQICDQKLEGGNLALERSMHYGIEVRVIRGMKYEGSASTSGKVYVYDGLYKIVSCLFDIGKSGFGVYKYKLLRIEGQAKMGSTVLKDARDIKKIGLDFMPMSCISIDISNNRENVPVRLFNDIDVNHEPLYYEYLPDSTFPWFVFHQSGEPSGCECKEACTDGCFCAMKNGGEFPYNLHGLLLKGKPLIFECGPFCSCPPNCRNRVAQKGLKYSLEVFRSTQTGWGVRSLDLIQAGSFICEYTGVAMTREQAQILTMNGGSLVYPSTFPNRWVEWGDLSQIYTEYVRPSYPSIPPLDYFLDVSTMRNVASYMSHSSSPNIFIQYVLYDHNNLLFPRVMLYAMENIPPMRELCIDYGIPDEWASKMSRCM*