AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044058

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044058 MtrunA17_Chr8g0364781 80.741 135 8 1 1 117 1 135 1.61e-72 212
MsaG044058 MtrunA17_Chr7g0262351 45.238 126 54 2 5 115 15 140 4.83e-27 97.4
MsaG044058 MtrunA17_Chr7g0262671 61.333 75 28 1 1 75 1 74 8.63e-26 92.8
MsaG044058 MtrunA17_Chr6g0472261 49.275 69 35 0 6 74 4 72 6.30e-16 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044058 AT5G26594.1 46.552 116 46 2 10 109 16 131 1.50e-23 88.6
MsaG044058 AT3G04280.1 40.541 111 48 3 12 104 22 132 4.05e-17 72.4
MsaG044058 AT3G04280.2 40.541 111 48 3 12 104 22 132 4.05e-17 72.4
MsaG044058 AT3G04280.3 40.541 111 48 3 12 104 22 132 4.05e-17 72.4

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATAGTGGTAAAATTTA+TGG 0.202240 8:-55268867 MsaT044058.1:CDS
CGTCATTTGATGAATTCTTC+GGG 0.320026 8:+55269469 None:intergenic
ACGTCATTTGATGAATTCTT+CGG 0.351042 8:+55269468 None:intergenic
CCTCACACAGTCACACTCTA+TGG 0.364334 8:-55268897 MsaT044058.1:intron
TATCCTTATCCATCAGGATT+AGG 0.413585 8:+55269363 None:intergenic
GACAACAAGTGCTGTTAACT+TGG 0.431046 8:+55269508 None:intergenic
GAAATAGTGGTAAAATTTAT+GGG 0.431369 8:-55268866 MsaT044058.1:CDS
TGACCTAATCCTGATGGATA+AGG 0.440174 8:-55269366 MsaT044058.1:CDS
CAGGCATATCCTTATCCATC+AGG 0.463932 8:+55269357 None:intergenic
GTGGTAAAATTTATGGGAGC+AGG 0.478217 8:-55268860 MsaT044058.1:CDS
CAAATGACGTTGAGTAACTT+AGG 0.479404 8:-55269455 MsaT044058.1:CDS
ACCTCAGTTCCATTCATCTC+AGG 0.498117 8:+55269338 None:intergenic
AAGGATATGCCTGAGATGAA+TGG 0.499813 8:-55269347 MsaT044058.1:CDS
AAAATTTGACCTAATCCTGA+TGG 0.515076 8:-55269372 MsaT044058.1:CDS
TATGGAAGAGGAGGAAATAG+TGG 0.524093 8:-55268879 MsaT044058.1:CDS
GCACTTGTTGTCGATGATGA+TGG 0.544588 8:-55269497 MsaT044058.1:CDS
CGAATATCTAGAGAAACCAT+TGG 0.558913 8:-55268831 MsaT044058.1:CDS
ACAGTCACACTCTATGGAAG+AGG 0.580170 8:-55268891 MsaT044058.1:intron
ACAGTGGAAATTCGTCAGCA+TGG 0.623061 8:-55269398 MsaT044058.1:CDS
GTCACACTCTATGGAAGAGG+AGG 0.628881 8:-55268888 MsaT044058.1:CDS
GTCATTTGATGAATTCTTCG+GGG 0.645477 8:+55269470 None:intergenic
GCCTGAGATGAATGGAACTG+AGG 0.719916 8:-55269339 MsaT044058.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 55268775 55269558 55268775 ID=MsaG044058
Chr8 mRNA 55268775 55269558 55268775 ID=MsaT044058.1;Parent=MsaG044058
Chr8 exon 55268775 55268909 55268775 ID=MsaT044058.1.exon2;Parent=MsaT044058.1
Chr8 CDS 55268775 55268909 55268775 ID=cds.MsaT044058.1;Parent=MsaT044058.1
Chr8 exon 55269340 55269558 55269340 ID=MsaT044058.1.exon1;Parent=MsaT044058.1
Chr8 CDS 55269340 55269558 55269340 ID=cds.MsaT044058.1;Parent=MsaT044058.1
Gene Sequence

>MsaT044058.1

ATGGCATCACCATCTGAAAACTTTGCATCCAAGTTAACAGCACTTGTTGTCGATGATGATGGAATGCCCCGAAGAATTCATCAAATGACGTTGAGTAACTTAGGAGTTAACAACACTTGTGTGAAAAACGGAAAAGAAACAGTGGAAATTCGTCAGCATGGTAAAAAATTTGACCTAATCCTGATGGATAAGGATATGCCTGAGATGAATGGAACTGAGTCACACTCTATGGAAGAGGAGGAAATAGTGGTAAAATTTATGGGAGCAGGACTCGACGAATATCTAGAGAAACCATTGGATAAAGCAAAACTCAATTCCATTCTTCATGCCGTTGAAAAAATAAAAGAAAAATAA
Protein sequence

>MsaT044058.1

MASPSENFASKLTALVVDDDGMPRRIHQMTLSNLGVNNTCVKNGKETVEIRQHGKKFDLILMDKDMPEMNGTESHSMEEEEIVVKFMGAGLDEYLEKPLDKAKLNSILHAVEKIKEK*