AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044082

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044082 MtrunA17_Chr8g0370121 87.083 240 27 3 1 237 3 241 3.03e-138 387
MsaG044082 MtrunA17_Chr8g0370131 50.612 245 91 8 1 238 3 224 2.24e-52 169
MsaG044082 MtrunA17_Chr6g0467271 56.140 114 39 4 1 104 3 115 1.50e-19 81.3
MsaG044082 MtrunA17_Chr5g0409841 40.299 67 40 0 91 157 87 153 2.71e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044082 AT5G08520.1 41.509 53 31 0 93 145 115 167 7.94e-11 61.2

Find 74 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGATTTGGAACTTCGTT+TGG 0.303203 8:-55645520 MsaT044082.1:CDS
CCATGATAGGTTGGAACAAT+AGG 0.309921 8:+55645633 None:intergenic
GTTAGCTTCTCCTCCATGAT+AGG 0.332362 8:+55645620 None:intergenic
TATTTGTGATGGGGATGATT+AGG 0.340678 8:-55645890 MsaT044082.1:CDS
CCTATTGTTCCAACCTATCA+TGG 0.371126 8:-55645633 MsaT044082.1:CDS
GATGAAGATATGGGTATTGA+TGG 0.385170 8:+55646826 None:intergenic
TGGTGCAACAGATGAAGATA+TGG 0.391943 8:+55646816 None:intergenic
TGTATTAGTTTATTTGTGAT+GGG 0.392456 8:-55645900 MsaT044082.1:intron
TGGTGTTGGTGGTGAAGATT+AGG 0.399314 8:+55646766 None:intergenic
TTGTATTAGTTTATTTGTGA+TGG 0.403857 8:-55645901 MsaT044082.1:intron
GAGCTGATGGTAAGGTAGTT+TGG 0.407255 8:+55646746 None:intergenic
ATTGATTTGGAACTTCGTTT+GGG 0.424529 8:-55645519 MsaT044082.1:CDS
ACTACCAACTGTCCTCAATC+TGG 0.424823 8:-55646691 MsaT044082.1:CDS
GAAAAGAGTGAGAGTTTCCT+TGG 0.431034 8:+55645656 None:intergenic
GAATCAAAATTATGTATCAT+TGG 0.435669 8:+55646796 None:intergenic
CCACCATTTGTTCCAGATTG+AGG 0.443069 8:+55646679 None:intergenic
GCTATGCTACTAGCTTCTTC+AGG 0.450363 8:-55645797 MsaT044082.1:CDS
TGTCCTCAATCTGGAACAAA+TGG 0.455413 8:-55646682 MsaT044082.1:CDS
AGTTGGTAGTGATCATTTGA+TGG 0.459982 8:+55646704 None:intergenic
GTCGGAGAACCCTAGCAATT+CGG 0.462131 8:+55646653 None:intergenic
AGTCCTTCTGCTGGTGTTGG+AGG 0.463490 8:-55645543 MsaT044082.1:CDS
GTAAGGTAGTTTGGTGTTGG+TGG 0.469673 8:+55646755 None:intergenic
AAAGAAAATATGATTCCAAC+GGG 0.472141 8:-55645740 MsaT044082.1:CDS
TGGTGTTGGAGGAATTGATT+TGG 0.474163 8:-55645532 MsaT044082.1:CDS
ATGGTAAGGTAGTTTGGTGT+TGG 0.479447 8:+55646752 None:intergenic
CATGATAGGTTGGAACAATA+GGG 0.479685 8:+55645634 None:intergenic
CGTAGTCCTTCTGCTGGTGT+TGG 0.483014 8:-55645546 MsaT044082.1:CDS
AAACCATGCACATATTCGTC+TGG 0.484230 8:+55645768 None:intergenic
TGCCATTCCCAAGTGGATCT+AGG 0.486137 8:-55646609 MsaT044082.1:CDS
TTAGGTATGGACAAGGGAGA+TGG 0.490096 8:-55645872 MsaT044082.1:CDS
AGTGCTTGCTTCATAGTTGT+TGG 0.490843 8:+55645581 None:intergenic
AGAGTGAGAGTTTCCTTGGC+AGG 0.497435 8:+55645660 None:intergenic
GATCTAGGCACATGGTGTCT+TGG 0.508481 8:-55646594 MsaT044082.1:CDS
AATGGCATGGTGTTTGATCC+TGG 0.510899 8:+55646625 None:intergenic
GCATAGCTTTGAACTTGTTG+TGG 0.515534 8:+55645813 None:intergenic
AGGGTTCTCCGACCGTTACC+AGG 0.518804 8:-55646643 MsaT044082.1:CDS
GCTTCTCCTCCATGATAGGT+TGG 0.531533 8:+55645624 None:intergenic
TGTTCCAGATTGAGGACAGT+TGG 0.540294 8:+55646687 None:intergenic
TGGTCGTGTCCTTGAGCTGA+TGG 0.542418 8:+55646733 None:intergenic
ACTCTGCGTAGTCCTTCTGC+TGG 0.546659 8:-55645552 MsaT044082.1:CDS
AAGGAAGTATGTAAGATCAA+TGG 0.547109 8:+55646858 None:intergenic
TGCCTAGATCCACTTGGGAA+TGG 0.547210 8:+55646607 None:intergenic
GTGTCCTTGAGCTGATGGTA+AGG 0.551347 8:+55646738 None:intergenic
AAAAGAAAATATGATTCCAA+CGG 0.555446 8:-55645741 MsaT044082.1:CDS
ATGGTGGTAGCCGAATTGCT+AGG 0.558071 8:-55646663 MsaT044082.1:CDS
GTTGGTAGTGATCATTTGAT+GGG 0.561211 8:+55646705 None:intergenic
GTAAGATCAATGGTCTCCAT+AGG 0.564153 8:+55646868 None:intergenic
GTATTAGTTTATTTGTGATG+GGG 0.567565 8:-55645899 MsaT044082.1:intron
GGTGCAACAGATGAAGATAT+GGG 0.573397 8:+55646817 None:intergenic
TGGTGGTAGCCGAATTGCTA+GGG 0.580753 8:-55646662 MsaT044082.1:CDS
GTGATGGGGATGATTAGGTA+TGG 0.581299 8:-55645885 MsaT044082.1:CDS
AGAAGGACTACGCAGAGTCA+TGG 0.582481 8:+55645557 None:intergenic
AGATATGGGTATTGATGGAG+TGG 0.594941 8:+55646831 None:intergenic
TGATCATTTGATGGGACTGA+TGG 0.601219 8:+55646713 None:intergenic
CCCAAGTGGATCTAGGCACA+TGG 0.602457 8:-55646602 MsaT044082.1:CDS
CATGCACATATTCGTCTGGT+AGG 0.603146 8:+55645772 None:intergenic
GTATTGATGGAGTGGAGTCA+AGG 0.606691 8:+55646839 None:intergenic
TTCTGACAATGATCCTGCCA+AGG 0.606818 8:-55645673 MsaT044082.1:CDS
ACCATGTGCCTAGATCCACT+TGG 0.620902 8:+55646601 None:intergenic
ATTCCTCCAACACCAGCAGA+AGG 0.622706 8:+55645540 None:intergenic
TTGGTGGTGAAGATTAGGAT+CGG 0.625676 8:+55646771 None:intergenic
AGATCCACTTGGGAATGGCA+TGG 0.630755 8:+55646612 None:intergenic
CCATGTGCCTAGATCCACTT+GGG 0.631806 8:+55646602 None:intergenic
CCTCAATCTGGAACAAATGG+TGG 0.632397 8:-55646679 MsaT044082.1:CDS
GGATGATGAGTTGATCCCGT+TGG 0.634114 8:+55645725 None:intergenic
ATGGTGTTTGATCCTGGTAA+CGG 0.649197 8:+55646631 None:intergenic
ACTACCTTACCATCAGCTCA+AGG 0.650911 8:-55646742 MsaT044082.1:CDS
TGTTTGATCCTGGTAACGGT+CGG 0.653269 8:+55646635 None:intergenic
AACACCATGCCATTCCCAAG+TGG 0.655209 8:-55646616 MsaT044082.1:CDS
GGGATGATTAGGTATGGACA+AGG 0.664435 8:-55645879 MsaT044082.1:CDS
CTACCAGACGAATATGTGCA+TGG 0.669323 8:-55645771 MsaT044082.1:CDS
ATGCATTGAATAATATGAAG+CGG 0.673343 8:+55645704 None:intergenic
GGATGATTAGGTATGGACAA+GGG 0.676501 8:-55645878 MsaT044082.1:CDS
ATTGTTCCAACCTATCATGG+AGG 0.738718 8:-55645630 MsaT044082.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 55645509 55646887 55645509 ID=MsaG044082
Chr8 mRNA 55645509 55646887 55645509 ID=MsaT044082.1;Parent=MsaG044082
Chr8 exon 55645509 55645914 55645509 ID=MsaT044082.1.exon2;Parent=MsaT044082.1
Chr8 CDS 55645509 55645914 55645509 ID=cds.MsaT044082.1;Parent=MsaT044082.1
Chr8 exon 55646577 55646887 55646577 ID=MsaT044082.1.exon1;Parent=MsaT044082.1
Chr8 CDS 55646577 55646887 55646577 ID=cds.MsaT044082.1;Parent=MsaT044082.1
Gene Sequence

>MsaT044082.1

ATGGAGACCATTGATCTTACATACTTCCTTGACTCCACTCCATCAATACCCATATCTTCATCTGTTGCACCAATGATACATAATTTTGATTCTACCGATCCTAATCTTCACCACCAACACCAAACTACCTTACCATCAGCTCAAGGACACGACCATCAGTCCCATCAAATGATCACTACCAACTGTCCTCAATCTGGAACAAATGGTGGTAGCCGAATTGCTAGGGTTCTCCGACCGTTACCAGGATCAAACACCATGCCATTCCCAAGTGGATCTAGGCACATGGTGTCTTGGAGTCAGACAGAGCATGATTTATTTGTGATGGGGATGATTAGGTATGGACAAGGGAGATGGAGTAAAATTGCTGAGAACTATGTATGCAACAAAACTCCACAACAAGTTCAAAGCTATGCTACTAGCTTCTTCAGGCACCTACCAGACGAATATGTGCATGGTTTAAAGAAAAGAAAATATGATTCCAACGGGATCAACTCATCATCCGCTTCATATTATTCAATGCATAACATGATTTCTGACAATGATCCTGCCAAGGAAACTCTCACTCTTTTCCCTATTGTTCCAACCTATCATGGAGGAGAAGCTAACAGCAGCAACAACAATACCAACAACTATGAAGCAAGCACTTCCATGACTCTGCGTAGTCCTTCTGCTGGTGTTGGAGGAATTGATTTGGAACTTCGTTTGGGTTTATCTTAG
Protein sequence

>MsaT044082.1

METIDLTYFLDSTPSIPISSSVAPMIHNFDSTDPNLHHQHQTTLPSAQGHDHQSHQMITTNCPQSGTNGGSRIARVLRPLPGSNTMPFPSGSRHMVSWSQTEHDLFVMGMIRYGQGRWSKIAENYVCNKTPQQVQSYATSFFRHLPDEYVHGLKKRKYDSNGINSSSASYYSMHNMISDNDPAKETLTLFPIVPTYHGGEANSSNNNTNNYEASTSMTLRSPSAGVGGIDLELRLGLS*