AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044215 MtrunA17_Chr8g0371601 97.613 1173 28 0 1 1173 1 1173 0.0 2384
MsaG044215 MtrunA17_Chr5g0405701 74.643 1191 254 14 4 1167 28 1197 0.0 1755
MsaG044215 MtrunA17_Chr1g0197591 29.449 472 300 11 280 734 197 652 1.52e-42 169
MsaG044215 MtrunA17_Chr1g0197591 39.231 130 65 4 1024 1153 921 1036 6.35e-17 86.7
MsaG044215 MtrunA17_Chr1g0194741 29.680 438 258 13 300 709 234 649 4.97e-38 152
MsaG044215 MtrunA17_Chr8g0377481 34.375 320 186 5 424 726 345 657 1.10e-36 150
MsaG044215 MtrunA17_Chr8g0377481 36.296 135 63 3 1026 1154 838 955 9.94e-17 85.9
MsaG044215 MtrunA17_Chr3g0122391 33.228 316 193 6 427 726 396 709 2.31e-35 146
MsaG044215 MtrunA17_Chr3g0122391 34.161 161 81 4 999 1154 872 1012 3.93e-17 87.4
MsaG044215 MtrunA17_Chr8g0392301 34.091 308 153 5 460 726 372 670 4.81e-35 145
MsaG044215 MtrunA17_Chr8g0392301 35.664 143 60 4 1026 1154 858 982 1.41e-16 85.5
MsaG044215 MtrunA17_Chr5g0447641 26.276 529 323 15 426 921 572 1066 1.60e-32 137
MsaG044215 MtrunA17_Chr5g0447641 31.944 144 61 4 1030 1154 1075 1200 1.28e-12 72.8
MsaG044215 MtrunA17_Chr7g0269631 32.031 256 152 3 461 716 334 567 4.92e-29 124
MsaG044215 MtrunA17_Chr4g0013831 31.321 265 159 4 461 725 334 575 6.54e-28 121
MsaG044215 MtrunA17_Chr3g0136601 31.985 272 164 5 474 726 373 642 2.28e-27 120
MsaG044215 MtrunA17_Chr1g0150511 27.154 383 215 10 405 726 298 677 7.04e-25 112
MsaG044215 MtrunA17_Chr1g0194731 29.665 209 123 7 946 1153 254 439 3.06e-17 86.3
MsaG044215 MtrunA17_Chr8g0343551 40.404 99 49 3 1071 1169 1 89 3.87e-14 68.9
MsaG044215 MtrunA17_Chr7g0274831 26.984 252 144 10 909 1153 527 745 2.31e-13 74.7
MsaG044215 MtrunA17_Chr7g0274831 24.036 337 213 9 460 784 364 669 7.69e-13 73.2
MsaG044215 MtrunA17_Chr1g0190021 22.785 395 249 14 460 837 360 715 8.21e-13 73.2
MsaG044215 MtrunA17_Chr1g0186431 29.496 139 97 1 461 599 355 492 1.97e-12 71.6
MsaG044215 MtrunA17_Chr8g0364781 31.690 142 72 4 1029 1167 12 131 2.31e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044215 AT2G17820.1 63.780 1201 373 17 8 1173 30 1203 0.0 1499
MsaG044215 AT2G47430.1 25.207 1206 683 44 57 1154 14 1108 4.90e-75 271
MsaG044215 AT2G01830.2 33.141 347 205 6 405 726 406 750 9.01e-41 164
MsaG044215 AT2G01830.2 36.735 147 67 4 1015 1154 932 1059 5.16e-17 87.4
MsaG044215 AT2G01830.5 33.141 347 205 6 405 726 395 739 9.62e-41 164
MsaG044215 AT2G01830.5 36.735 147 67 4 1015 1154 921 1048 5.59e-17 87.4
MsaG044215 AT2G01830.1 33.141 347 205 6 405 726 383 727 9.64e-41 164
MsaG044215 AT2G01830.1 36.735 147 67 4 1015 1154 909 1036 5.51e-17 87.4
MsaG044215 AT2G01830.4 33.141 347 205 6 405 726 383 727 9.64e-41 164
MsaG044215 AT2G01830.4 36.735 147 67 4 1015 1154 909 1036 5.51e-17 87.4
MsaG044215 AT2G01830.6 33.141 347 205 6 405 726 383 727 9.64e-41 164
MsaG044215 AT2G01830.6 36.735 147 67 4 1015 1154 909 1036 5.51e-17 87.4
MsaG044215 AT2G01830.3 33.141 347 205 6 405 726 383 727 9.64e-41 164
MsaG044215 AT2G01830.3 36.735 147 67 4 1015 1154 909 1036 5.51e-17 87.4
MsaG044215 AT1G27320.1 35.294 323 195 4 405 716 384 703 5.91e-40 161
MsaG044215 AT1G27320.1 33.333 147 63 3 1026 1154 887 1016 4.16e-14 77.8
MsaG044215 AT5G35750.1 32.919 322 193 6 424 726 540 857 4.60e-37 152
MsaG044215 AT5G35750.1 29.605 152 70 4 1030 1162 1036 1169 4.80e-13 74.7
MsaG044215 AT1G66340.1 32.031 256 148 2 461 716 333 562 8.80e-31 131
MsaG044215 AT5G10720.1 32.946 258 146 6 474 726 369 604 1.65e-27 121
MsaG044215 AT2G40940.1 31.373 255 153 3 460 714 332 564 1.30e-24 110
MsaG044215 AT3G04580.2 35.664 143 72 4 1021 1162 634 757 1.54e-14 79.0
MsaG044215 AT3G04580.1 35.664 143 72 4 1021 1162 634 757 1.54e-14 79.0

Find 215 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAAGATGATTCATATTT+TGG 0.108507 8:-57323737 MsaT044215.1:CDS
ACCTCATATTTGCAAGAAAT+TGG 0.164195 8:+57326281 None:intergenic
CAGAAAAGTAACTGCTTATT+AGG 0.180290 8:-57323499 MsaT044215.1:CDS
TCACTATCACCATAAGAATT+TGG 0.199041 8:+57325374 None:intergenic
GTTCTCATGTTGCTTCATTT+TGG 0.222254 8:+57325307 None:intergenic
CCAAGATGTTATTGAGTAAA+TGG 0.227661 8:+57326021 None:intergenic
CTTGCGAAGCTCCGTCTTTA+CGG 0.241218 8:+57323798 None:intergenic
TAGGTATTGACCCGAGTAAA+TGG 0.298638 8:-57325038 MsaT044215.1:CDS
GCCTCATATCCATCCATCTT+TGG 0.303088 8:+57323006 None:intergenic
TTCAGCAGCATTGCCGGTTT+GGG 0.306475 8:-57327593 MsaT044215.1:CDS
CATACCAAGAAAGCATATAA+TGG 0.311598 8:-57326494 MsaT044215.1:CDS
GAAATAAGGAAATCAGAAAT+TGG 0.311793 8:-57322976 MsaT044215.1:CDS
AAAGAAACCTGAGCTTAAAA+TGG 0.319263 8:-57327398 MsaT044215.1:CDS
TTTCAGCAGCATTGCCGGTT+TGG 0.320446 8:-57327594 MsaT044215.1:CDS
CCTGTCAATGGCGAGAAAAT+TGG 0.321831 8:-57327733 MsaT044215.1:CDS
AACAAAGTCTCTAAATAGTT+TGG 0.322886 8:-57328251 MsaT044215.1:CDS
TTCATATGACTTGATCCTAA+TGG 0.325693 8:-57323158 MsaT044215.1:CDS
TTCTCTTTGTTTGTCTCCAT+TGG 0.328818 8:+57323555 None:intergenic
GCCAATTTCTTGCAAATATG+AGG 0.329015 8:-57326282 MsaT044215.1:intron
GCTATACTTATTGAGTCTTC+TGG 0.329518 8:+57327691 None:intergenic
AGTCAAATTCTGCCTCTTCT+AGG 0.333858 8:+57325837 None:intergenic
AGAAATCAAGGTTGTCAAAA+AGG 0.334329 8:-57324369 MsaT044215.1:CDS
CATGCCCACTTCTAAACATT+TGG 0.335841 8:+57322894 None:intergenic
ATACCAAGAAAGCATATAAT+GGG 0.337592 8:-57326493 MsaT044215.1:CDS
CCGCCTTTCTTCTTGCTTCG+AGG 0.341358 8:+57326326 None:intergenic
ATGATATGCCAAAGCTAGTT+CGG 0.350156 8:-57325647 MsaT044215.1:CDS
ATGAGGCAACAAAAGAAATA+AGG 0.352677 8:-57322990 MsaT044215.1:CDS
TAGACTTGAGCACAGATATA+TGG 0.354728 8:-57323900 MsaT044215.1:CDS
TATAATCGCATTAGTGTTCC+TGG 0.355538 8:+57324341 None:intergenic
GGTTCTGACATATCTGAAAT+TGG 0.360389 8:-57323598 MsaT044215.1:CDS
GACATTGAGTAGTTGAATAC+TGG 0.372323 8:+57328841 None:intergenic
TCTTTGTGAAATGCCATGTT+AGG 0.374134 8:+57328281 None:intergenic
TGATATGCCAAAGCTAGTTC+GGG 0.388131 8:-57325646 MsaT044215.1:CDS
CAGGCAGTGGATGCTCTAAA+TGG 0.388139 8:-57323238 MsaT044215.1:CDS
GTTACTTTCAGCAGCATTGC+CGG 0.388211 8:-57327599 MsaT044215.1:CDS
CTTATTACAGGTTAATAAGA+TGG 0.389612 8:-57324396 MsaT044215.1:intron
AGGCACGGAGGCACTGGTCT+TGG 0.389721 8:-57324802 MsaT044215.1:CDS
TTCTAAATTTATTAGCATAC+AGG 0.393462 8:-57323968 MsaT044215.1:CDS
TATAGCACATAGGTGGAATC+TGG 0.394834 8:-57325869 MsaT044215.1:intron
AAGCTCCGTCTTTACGGTAT+TGG 0.396922 8:+57323804 None:intergenic
ATCATCTTTGCTTTGTAAAG+AGG 0.401741 8:+57323748 None:intergenic
TCCATCAGCTTAAAGTCAAT+TGG 0.402940 8:+57322853 None:intergenic
TGTGAAAATCCAAATTCTTA+TGG 0.403470 8:-57325383 MsaT044215.1:CDS
TATTAGAGGTCTTCTTTGAA+TGG 0.404156 8:+57325285 None:intergenic
ATCCGAATTGCTTCTGCTCC+AGG 0.406373 8:+57325342 None:intergenic
CCATTTACTCAATAACATCT+TGG 0.410502 8:-57326021 MsaT044215.1:CDS
TTCATATGCTGCAAATGAAT+TGG 0.417578 8:+57327804 None:intergenic
CTTGATCCTAATGGATTGTC+AGG 0.421407 8:-57323149 MsaT044215.1:intron
TCAACAACAATGACAAATGT+TGG 0.424662 8:+57323934 None:intergenic
GAATTCAGACCCTCCGGAGC+TGG 0.424803 8:+57323988 None:intergenic
TCATCATTGTTAAGATTTCA+AGG 0.425847 8:+57322798 None:intergenic
ACACCGATGGCAGCAGTAAT+CGG 0.426125 8:-57326134 MsaT044215.1:CDS
GAATCTCCCCGAACTAGCTT+TGG 0.429030 8:+57325639 None:intergenic
ACAATGACAAATGTTGGGTT+TGG 0.429280 8:+57323940 None:intergenic
AAAAGCATTGTTGCAGTTGT+GGG 0.430753 8:-57327559 MsaT044215.1:CDS
TTTATTTCAACAAGAGCAAA+AGG 0.434209 8:-57323442 MsaT044215.1:CDS
TGCTGTAGGCGACGGACAAC+AGG 0.439996 8:-57323257 MsaT044215.1:CDS
AGAAGTCTCTTGAAGGTTTG+AGG 0.441819 8:-57323357 MsaT044215.1:CDS
ATAGCAACAAGTCTGCGATT+TGG 0.446888 8:-57327774 MsaT044215.1:CDS
GTTATGCTAGCTATCTTGAT+TGG 0.449619 8:-57328316 MsaT044215.1:CDS
AAGACGGAGCTTCGCAAGAA+AGG 0.451119 8:-57323793 MsaT044215.1:CDS
TGACAGGATCAAGTTGTTCC+CGG 0.454045 8:+57327749 None:intergenic
GACTTTGGAAGGGAACTTGA+AGG 0.454376 8:-57325818 MsaT044215.1:CDS
CAACAACAATGACAAATGTT+GGG 0.456008 8:+57323935 None:intergenic
AGGCAGAATTTGACTTTGGA+AGG 0.465133 8:-57325829 MsaT044215.1:CDS
CATTTCAGGCACGGAGGCAC+TGG 0.467371 8:-57324808 MsaT044215.1:intron
TAAAAGCATTGTTGCAGTTG+TGG 0.467925 8:-57327560 MsaT044215.1:CDS
CAGCTTAAAGTCAATTGGCT+TGG 0.468501 8:+57322858 None:intergenic
ACGCGAGAGCGAAATTTGTA+TGG 0.470371 8:-57323846 MsaT044215.1:CDS
GCCAATTGACTTTAAGCTGA+TGG 0.471034 8:-57322854 MsaT044215.1:CDS
ATGAGTCAATGTAATATTGC+TGG 0.473523 8:+57327260 None:intergenic
ACTCGATAACTGTCAAGTAT+AGG 0.475293 8:-57327909 MsaT044215.1:CDS
GAAGAGGCAGAATTTGACTT+TGG 0.476112 8:-57325833 MsaT044215.1:CDS
AGCATTTCATAGAGACTTGA+AGG 0.476420 8:-57327872 MsaT044215.1:CDS
CGTGGTTCGCCTAGCAATCA+TGG 0.479373 8:-57328796 MsaT044215.1:intron
ACCCTGGAGCAGAAGCAATT+CGG 0.480583 8:-57325344 MsaT044215.1:CDS
ATTAGGATCAAGTCATATGA+AGG 0.480841 8:+57323160 None:intergenic
TGATGCTCACTTTCCAGCTC+CGG 0.483762 8:-57324001 MsaT044215.1:CDS
CACATACCTGACAATCCATT+AGG 0.485467 8:+57323143 None:intergenic
ATATTGCTGGTGACCTAGCC+TGG 0.485638 8:+57327273 None:intergenic
AAGAAAATTCCACAAGGATG+TGG 0.485997 8:-57328877 MsaT044215.1:CDS
CAAGTCTGCGATTTGGTACC+GGG 0.486373 8:-57327767 MsaT044215.1:CDS
GAAGACTCAATAAGTATAGC+TGG 0.486565 8:-57327688 MsaT044215.1:CDS
ATCCTACTACAGTGTCTTCG+TGG 0.488058 8:-57328814 MsaT044215.1:CDS
TCACTGTGTATTATCGTCAT+TGG 0.491172 8:-57326421 MsaT044215.1:CDS
GCCAAAGATGGATGGATATG+AGG 0.491952 8:-57323007 MsaT044215.1:CDS
AACACAGAATCCCATTTACT+CGG 0.494050 8:+57325027 None:intergenic
CTAAAATAATTTCTACATTG+TGG 0.494150 8:+57325762 None:intergenic
AATGGAAAGAAGTCTCTTGA+AGG 0.494792 8:-57323364 MsaT044215.1:CDS
AGCTTCGTGGCACGTTTCTG+TGG 0.494812 8:-57327641 MsaT044215.1:CDS
ACAAGTCTGCGATTTGGTAC+CGG 0.495830 8:-57327768 MsaT044215.1:CDS
TCTTTGCATTGTCAGATCCT+TGG 0.496165 8:-57324779 MsaT044215.1:intron
TGCCACGAAGCTACACCGTC+AGG 0.496443 8:+57327652 None:intergenic
AGTGAAACAAGTAGGATTAG+TGG 0.496758 8:-57323403 MsaT044215.1:CDS
TGCCCATGTGACAGCCCTCA+TGG 0.498535 8:+57327957 None:intergenic
TAAGATGGGTGGAGAAATCA+AGG 0.499777 8:-57324381 MsaT044215.1:CDS
TCATGAACCTCATGACTTGG+AGG 0.502697 8:+57327304 None:intergenic
TTGATAACAAAATGATACTC+TGG 0.503219 8:-57325262 MsaT044215.1:CDS
TAGTGTAGGGCAGTTAATGA+AGG 0.504796 8:-57327518 MsaT044215.1:CDS
TCAAGTCTCTATGAAATGCT+TGG 0.505424 8:+57327875 None:intergenic
TGCTCACTTTCCAGCTCCGG+AGG 0.508924 8:-57323998 MsaT044215.1:CDS
AGGTATTGACCCGAGTAAAT+GGG 0.509028 8:-57325037 MsaT044215.1:CDS
TCTTCATGAACCTCATGACT+TGG 0.512203 8:+57327301 None:intergenic
TTTGTTGAATTTGTAAGTAG+AGG 0.513089 8:+57327418 None:intergenic
AAGATACAGCAGTAATTCAA+AGG 0.514361 8:-57323321 MsaT044215.1:CDS
AATATTCCACATCCTCAAGA+TGG 0.515647 8:+57328195 None:intergenic
ATTCCATTCAGATGCTTCCA+TGG 0.518500 8:+57324086 None:intergenic
GGTTCAAGTAAAGCTATGAA+TGG 0.518559 8:-57323382 MsaT044215.1:CDS
CCACCATGGAAGCATCTGAA+TGG 0.519665 8:-57324089 MsaT044215.1:CDS
TCTAATCTTGTAACTGCCAA+TGG 0.521244 8:-57323571 MsaT044215.1:CDS
GAACTAGTTGATAAGCATAG+TGG 0.522807 8:-57327496 MsaT044215.1:CDS
TACCTGACGGTGTAGCTTCG+TGG 0.523588 8:-57327654 MsaT044215.1:CDS
ATGTGGCTAAAGAAAACATA+CGG 0.523995 8:-57327337 MsaT044215.1:CDS
CAACTGCACTTTATAGTGTA+GGG 0.526485 8:-57327531 MsaT044215.1:CDS
TTATTACAGGTTAATAAGAT+GGG 0.527467 8:-57324395 MsaT044215.1:intron
AATAAAGCAGATGCCAAAGA+TGG 0.528045 8:-57323019 MsaT044215.1:intron
ACAACTGCACTTTATAGTGT+AGG 0.528302 8:-57327532 MsaT044215.1:CDS
ATCCATGAGGGCTGTCACAT+GGG 0.532393 8:-57327959 MsaT044215.1:CDS
TTACGGTATTGGACGAGTCA+TGG 0.532764 8:+57323815 None:intergenic
TGTGATGTTGCAAGCTCAGA+TGG 0.534189 8:-57323619 MsaT044215.1:CDS
ATTAACTGACCATGATTGCT+AGG 0.534354 8:+57328787 None:intergenic
GATGCTCTAAATGGCATGCC+TGG 0.535325 8:-57323229 MsaT044215.1:CDS
GTAGATTAATTACATCTAAG+TGG 0.536265 8:-57324131 MsaT044215.1:CDS
TTATTAGAAGAATCCCAAAC+CGG 0.537322 8:+57327580 None:intergenic
CACCGTCAGGTACTTGAGAC+AGG 0.538434 8:+57327665 None:intergenic
TTGTTGAATTTGTAAGTAGA+GGG 0.538960 8:+57327419 None:intergenic
CATGAAGAGAACGCCAGGCT+AGG 0.542087 8:-57327286 MsaT044215.1:CDS
GCTTCGTGGCACGTTTCTGT+GGG 0.544714 8:-57327640 MsaT044215.1:CDS
GAACAACTTGATCCTGTCAA+TGG 0.544898 8:-57327745 MsaT044215.1:CDS
AATTTAGAATTCAGACCCTC+CGG 0.545642 8:+57323982 None:intergenic
TGTAAGTAGAGGGTCATTTG+TGG 0.547760 8:+57327429 None:intergenic
TGAAAGAGCCTTCAAAACAT+TGG 0.549125 8:-57326461 MsaT044215.1:CDS
GCAAAAGGTGAAGATTCTAA+AGG 0.549796 8:-57323427 MsaT044215.1:CDS
ACTTTGGAAGGGAACTTGAA+GGG 0.550352 8:-57325817 MsaT044215.1:CDS
GAAGTTGACGACACAGGCTG+TGG 0.553420 8:-57325236 MsaT044215.1:intron
ATTGACAGATGGAGTATCAA+AGG 0.553958 8:-57326380 MsaT044215.1:CDS
AGGTTCATGAAGAGAACGCC+AGG 0.554398 8:-57327291 MsaT044215.1:CDS
AAGCAGATGCCAAAGATGGA+TGG 0.554555 8:-57323015 MsaT044215.1:intron
AATCCATGAGGGCTGTCACA+TGG 0.555825 8:-57327960 MsaT044215.1:CDS
AACTAGTTGATAAGCATAGT+GGG 0.557926 8:-57327495 MsaT044215.1:CDS
GGTCTCATTATATGAAAGAT+CGG 0.560737 8:+57327825 None:intergenic
TGCTTATTAGGATTAAGTAA+TGG 0.563159 8:-57323487 MsaT044215.1:CDS
AACGTCCCATCTTGAGGATG+TGG 0.563982 8:-57328201 MsaT044215.1:CDS
ATGGAAGCATCTGAATGGAA+TGG 0.564124 8:-57324084 MsaT044215.1:CDS
AGATACAGCAGTAATTCAAA+GGG 0.566152 8:-57323320 MsaT044215.1:CDS
TCGGGGAGATTCTGCGAGAG+TGG 0.566872 8:-57325628 MsaT044215.1:CDS
CAGGTACTTCTTGCACTGCA+TGG 0.569416 8:-57324162 MsaT044215.1:CDS
GGCAGAATTTGACTTTGGAA+GGG 0.570782 8:-57325828 MsaT044215.1:CDS
ATTGTGTTTCTTTCAACACC+AGG 0.570876 8:+57323211 None:intergenic
AGGCGACGGACAACAGGCAG+TGG 0.572133 8:-57323251 MsaT044215.1:CDS
CATGAACCTCATGACTTGGA+GGG 0.573555 8:+57327305 None:intergenic
GTCACATTATTCTGAGAGGA+TGG 0.574036 8:-57325406 MsaT044215.1:CDS
TCCGAATTGCTTCTGCTCCA+GGG 0.576134 8:+57325343 None:intergenic
TACTGCAACGTCCCATCTTG+AGG 0.578797 8:-57328207 MsaT044215.1:CDS
GGAGCCACCGTTGTTGCTGT+AGG 0.580845 8:-57323271 MsaT044215.1:CDS
ATAACTGTCAAGTATAGGAA+TGG 0.583037 8:-57327904 MsaT044215.1:CDS
AGGATTAAGTAATGGATACA+TGG 0.583551 8:-57323479 MsaT044215.1:CDS
TCACCTCGAAGCAAGAAGAA+AGG 0.583647 8:-57326329 MsaT044215.1:CDS
AATCCGATTACTGCTGCCAT+CGG 0.585511 8:+57326131 None:intergenic
CATGCGACTAACTGCACCCG+TGG 0.587768 8:-57324318 MsaT044215.1:CDS
TGAGCTGTAAGAGCGACGAT+TGG 0.588069 8:+57322937 None:intergenic
CGAAGCAAGAAGAAAGGCGG+AGG 0.588877 8:-57326323 MsaT044215.1:CDS
AAATAAGATTCATACCAGAA+AGG 0.590579 8:+57325738 None:intergenic
ACCGTTGTTGCTGTAGGCGA+CGG 0.591122 8:-57323265 MsaT044215.1:CDS
CTCTCGCGTAGTACTTGTGA+AGG 0.591922 8:+57323860 None:intergenic
TGGAAAACTTGTCCTAGAAG+AGG 0.595077 8:-57325849 MsaT044215.1:CDS
ATGTTGTTCAGTGGTATCCA+CGG 0.595253 8:+57324301 None:intergenic
CTAAAGGTAGTGAAACAAGT+AGG 0.597373 8:-57323411 MsaT044215.1:CDS
TGAAGCCAAATGTTTAGAAG+TGG 0.598691 8:-57322899 MsaT044215.1:CDS
ATATTCCACATCCTCAAGAT+GGG 0.599048 8:+57328196 None:intergenic
AACCACGAAGACACTGTAGT+AGG 0.600067 8:+57328812 None:intergenic
TGTTGTTCAGTGGTATCCAC+GGG 0.604814 8:+57324302 None:intergenic
TTACAGGTTAATAAGATGGG+TGG 0.605671 8:-57324392 MsaT044215.1:intron
TGGAGACAAACAAAGAGAAG+AGG 0.609301 8:-57323551 MsaT044215.1:CDS
TGGTTTGAAGTTGACGACAC+AGG 0.609444 8:-57325242 MsaT044215.1:CDS
GGCCTGTCTCAAGTACCTGA+CGG 0.611371 8:-57327667 MsaT044215.1:CDS
CTCGTCCAATACCGTAAAGA+CGG 0.614421 8:-57323809 MsaT044215.1:CDS
GCTCACTTTCCAGCTCCGGA+GGG 0.614527 8:-57323997 MsaT044215.1:CDS
TATGTATTTGACCTCTCAAG+AGG 0.615158 8:-57327470 MsaT044215.1:CDS
CAAATGTTTAGAAGTGGGCA+TGG 0.615383 8:-57322893 MsaT044215.1:CDS
GAGTATGTTATCAGAAGCCA+CGG 0.615717 8:-57328127 MsaT044215.1:CDS
TAACATCTTGGATATAAGCA+AGG 0.616190 8:-57326009 MsaT044215.1:intron
GAAGCCAAATGTTTAGAAGT+GGG 0.619807 8:-57322898 MsaT044215.1:CDS
CAATTTCCCTCCAAGTCATG+AGG 0.619846 8:-57327311 MsaT044215.1:CDS
TACAGCAGTAATTCAAAGGG+TGG 0.626931 8:-57323317 MsaT044215.1:CDS
GACTTGACAATGTTGTTCAG+TGG 0.628126 8:+57324292 None:intergenic
TTAGGATCAAGTCATATGAA+GGG 0.630200 8:+57323161 None:intergenic
ATAATCGCATTAGTGTTCCT+GGG 0.633707 8:+57324342 None:intergenic
TACCAAGAAAGCATATAATG+GGG 0.634950 8:-57326492 MsaT044215.1:CDS
TTTGAGTGGCTAGGTTACCG+TGG 0.635843 8:+57328110 None:intergenic
GGCAATGCTGCTGAAAGTAA+CGG 0.637366 8:+57327601 None:intergenic
TAAGAGCGACGATTGGAATG+TGG 0.639382 8:+57322944 None:intergenic
TGATAACGAAGTAATAAGAG+AGG 0.639587 8:-57327365 MsaT044215.1:CDS
GTCTACTCACTATCCTAACA+TGG 0.643699 8:-57328294 MsaT044215.1:CDS
GATAACGAAGTAATAAGAGA+GGG 0.644016 8:-57327364 MsaT044215.1:CDS
ACACAGAATCCCATTTACTC+GGG 0.644528 8:+57325028 None:intergenic
CCATTCAGATGCTTCCATGG+TGG 0.651094 8:+57324089 None:intergenic
AAGCAAATAGCCCTCTTGAG+AGG 0.654817 8:+57327459 None:intergenic
TGATAGTGAAAATTCTACCC+TGG 0.657747 8:-57325360 MsaT044215.1:CDS
ATGTATTTGACCTCTCAAGA+GGG 0.661239 8:-57327469 MsaT044215.1:CDS
ACCAAGAAAGCATATAATGG+GGG 0.661897 8:-57326491 MsaT044215.1:CDS
TGTGGCTAAAGAAAACATAC+GGG 0.667227 8:-57327336 MsaT044215.1:CDS
TCCGTCGCCTACAGCAACAA+CGG 0.667951 8:+57323264 None:intergenic
TCATGAACTGAGGACACCGA+TGG 0.669919 8:-57326147 MsaT044215.1:CDS
TGCAGATGTATGAATCCATG+AGG 0.670113 8:-57327972 MsaT044215.1:intron
GCTGGTCACATTATTCTGAG+AGG 0.685346 8:-57325410 MsaT044215.1:CDS
CCTCGAAGCAAGAAGAAAGG+CGG 0.694393 8:-57326326 MsaT044215.1:CDS
TGCATTACAGTCATGAACTG+AGG 0.713029 8:-57326157 MsaT044215.1:intron
TAATAAGAGAGGGAGCGATG+TGG 0.713072 8:-57327354 MsaT044215.1:CDS
GATATGCCAAAGCTAGTTCG+GGG 0.714157 8:-57325645 MsaT044215.1:CDS
AGGCAGAAGAAAATTCCACA+AGG 0.721322 8:-57328883 MsaT044215.1:CDS
GTCGCCTACAGCAACAACGG+TGG 0.727532 8:+57323267 None:intergenic
GCAGATGTATGAATCCATGA+GGG 0.735980 8:-57327971 MsaT044215.1:intron
GTTGCAGTAGTTCATAACGA+AGG 0.740407 8:+57328221 None:intergenic
ATTGAGTAAATGGAGCAATG+CGG 0.793121 8:+57326031 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 57322807 57328985 57322807 ID=MsaG044215
Chr8 mRNA 57322807 57328985 57322807 ID=MsaT044215.1;Parent=MsaG044215
Chr8 exon 57322807 57323031 57322807 ID=MsaT044215.1.exon13;Parent=MsaT044215.1
Chr8 CDS 57322807 57323031 57322807 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57323150 57324181 57323150 ID=MsaT044215.1.exon12;Parent=MsaT044215.1
Chr8 CDS 57323150 57324181 57323150 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57324268 57324408 57324268 ID=MsaT044215.1.exon11;Parent=MsaT044215.1
Chr8 CDS 57324268 57324408 57324268 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57324780 57324822 57324780 ID=MsaT044215.1.exon10;Parent=MsaT044215.1
Chr8 CDS 57324780 57324822 57324780 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57324988 57325057 57324988 ID=MsaT044215.1.exon9;Parent=MsaT044215.1
Chr8 CDS 57324988 57325057 57324988 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57325237 57325431 57325237 ID=MsaT044215.1.exon8;Parent=MsaT044215.1
Chr8 CDS 57325237 57325431 57325237 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57325580 57325669 57325580 ID=MsaT044215.1.exon7;Parent=MsaT044215.1
Chr8 CDS 57325580 57325669 57325580 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57325753 57325879 57325753 ID=MsaT044215.1.exon6;Parent=MsaT044215.1
Chr8 CDS 57325753 57325879 57325753 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57326010 57326169 57326010 ID=MsaT044215.1.exon5;Parent=MsaT044215.1
Chr8 CDS 57326010 57326169 57326010 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57326283 57326530 57326283 ID=MsaT044215.1.exon4;Parent=MsaT044215.1
Chr8 CDS 57326283 57326530 57326283 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57327231 57327989 57327231 ID=MsaT044215.1.exon3;Parent=MsaT044215.1
Chr8 CDS 57327231 57327989 57327231 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57328096 57328338 57328096 ID=MsaT044215.1.exon2;Parent=MsaT044215.1
Chr8 CDS 57328096 57328338 57328096 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Chr8 exon 57328797 57328985 57328797 ID=MsaT044215.1.exon1;Parent=MsaT044215.1
Chr8 CDS 57328797 57328985 57328797 ID=cds.MsaT044215.1;Parent=MsaT044215.1
Gene Sequence

>MsaT044215.1

ATGGCTACCAAGTGCATGCACGTTTTTGATAGATTGCTTCTGTGTTTTGCCACTTGTTGGAAGAAAAATAGCACACTAACAGGCAGAAGAAAATTCCACAAGGATGTGGAAAAAGAAGAATTCCAGTATTCAACTACTCAATGTCTCTCATCCTACTACAGTGTCTTCGTGGTTCGCCTAGCAATCATGGTTATGCTAGCTATCTTGATTGGTCTACTCACTATCCTAACATGGCATTTCACAAAGATTTATACAACAAAGTCTCTAAATAGTTTGGCTTACGACCTTCGTTATGAACTACTGCAACGTCCCATCTTGAGGATGTGGAATATTTTGAACTCTACTGCTGAAATCACAACTGCTCAAGTTAAGTTGTCTGAGTATGTTATCAGAAGCCACGGTAACCTAGCCACTCAAACAGAAAAAGTTGAGATGTATGAATCCATGAGGGCTGTCACATGGGCACTGTTTGCTAGTCGAAAAGCTCTCAACTCGATAACTGTCAAGTATAGGAATGGTTTTGTCCAAGCATTTCATAGAGACTTGAAGGATAATAATATTTTTTACATCTACACCGATCTTTCATATAATGAGACCAATTCATTTGCAGCATATGAAGACACTCATAGCAACAAGTCTGCGATTTGGTACCGGGAACAACTTGATCCTGTCAATGGCGAGAAAATTGGAAAAGCAATGAAAATTGCACCAGAAGACTCAATAAGTATAGCTGGCCTGTCTCAAGTACCTGACGGTGTAGCTTCGTGGCACGTTTCTGTGGGCAAGTTTACAGATTCACCGTTACTTTCAGCAGCATTGCCGGTTTGGGATTCTTCTAATAAAAGCATTGTTGCAGTTGTGGGAGTTACAACTGCACTTTATAGTGTAGGGCAGTTAATGAAGGAACTAGTTGATAAGCATAGTGGGCATATGTATTTGACCTCTCAAGAGGGCTATTTGCTTGCAACTTCCACAAATGACCCTCTACTTACAAATTCAACAAAGAAACCTGAGCTTAAAATGGCTGTTGACTGTGATAACGAAGTAATAAGAGAGGGAGCGATGTGGCTAAAGAAAACATACGGGAACAATTTCCCTCCAAGTCATGAGGTTCATGAAGAGAACGCCAGGCTAGGTCACCAGCAATATTACATTGACTCATTCTTCCTAATCTTGAAAAAGCTTCCTTTGGTGGGTGTAATAATCATACCAAGAAAGCATATAATGGGGGAGGCAGATGAAAGAGCCTTCAAAACATTGGTTATTCTGATATCTGCATCACTGTGTATTATCGTCATTGGATGTGTTTGCATTTTGATATTGACAGATGGAGTATCAAAGGAAATGAATCTAAGAGCAGAATTAATCAGTCACCTCGAAGCAAGAAGAAAGGCGGAGGCATCGAGCAACTATAAAAGCCAATTTCTTGCAAATATGAGTCATGAACTGAGGACACCGATGGCAGCAGTAATCGGATTACTTGACATTCTTTTGTCGGATGATCATCTCACAAATGAACAATGTGCAACCGTTACTCAAATAAGAAAATGTTCAACCGCATTGCTCCATTTACTCAATAACATCTTGGATATAAGCAAGGTGGAATCTGGAAAACTTGTCCTAGAAGAGGCAGAATTTGACTTTGGAAGGGAACTTGAAGGGCTTGTAGATATGTTTTCTGTGCAATGCATTAACCACAATGTAGAAATTATTTTAGACCTTTCTGATGATATGCCAAAGCTAGTTCGGGGAGATTCTGCGAGAGTGGTTCAAATTTTTGCAAATTTGATCAATAACTCAATCAAATTTACTCTATCTGGTCACATTATTCTGAGAGGATGGTGTGAAAATCCAAATTCTTATGGTGATAGTGAAAATTCTACCCTGGAGCAGAAGCAATTCGGATGTTCTCAGAAGACCAAAATGAAGCAACATGAGAACCATTCAAAGAAGACCTCTAATATTGATAACAAAATGATACTCTGGTTTGAAGTTGACGACACAGGCTGTGGTATTGACCCGAGTAAATGGGATTCTGTGTTTGAAAGCTTTGAGCAAGCTGATCTATCAACTACTAGACTGCACGGAGGCACTGGTCTTGGTCTTTGCATTGTCAGATCCTTGGTTAATAAGATGGGTGGAGAAATCAAGGTTGTCAAAAAGGAAGGCCCAGGAACACTAATGCGATTATACATGCGACTAACTGCACCCGTGGATACCACTGAACAACATTGTCAAGTCGATTTTGCTAACAATAACTTAGTGGTACTTCTTGCACTGCATGGCAACATGAGTAGATTAATTACATCTAAGTGGTTACAGAAAAACGGCGTGGCCACCATGGAAGCATCTGAATGGAATGGATTAACACAAATTCTGAAAGAACTATTTCGTGCAAAAACTTCAACTCATAACAATGACTTTGATGCTCACTTTCCAGCTCCGGAGGGTCTGAATTCTAAATTTATTAGCATACAGGAATTGCCAAACCCAACATTTGTCATTGTTGTTGATATTGATCTACTAGACTTGAGCACAGATATATGGAAAGAACAACTTAACTTCCTTCACAAGTACTACGCGAGAGCGAAATTTGTATGGTTACAAAACCATGACTCGTCCAATACCGTAAAGACGGAGCTTCGCAAGAAAGGACATATACTAACTGTCAACAAACCTCTTTACAAAGCAAAGATGATTCATATTTTGGACGACGTCATAAAGGAAAGAAACGTTGAAGTACAAAAGAAAAATATGAAAGAGGATGATTTGCATGAGAGTCTTGAGATTGAATATACTCATCATTGTGATGTTGCAAGCTCAGATGGTTCTGACATATCTGAAATTGGTAGTTCTAATCTTGTAACTGCCAATGGAGACAAACAAAGAGAAGAGGTAGTTAGAATCAATCCATCGTCACTATACCAGAAAAGTAACTGCTTATTAGGATTAAGTAATGGATACATGGAACATAAAGAAGCATTTATTTCAACAAGAGCAAAAGGTGAAGATTCTAAAGGTAGTGAAACAAGTAGGATTAGTGGTTCAAGTAAAGCTATGAATGGAAAGAAGTCTCTTGAAGGTTTGAGGATATTGCTTGCAGAAGATACAGCAGTAATTCAAAGGGTGGCAACCATAATGCTTGAAAAAATGGGAGCCACCGTTGTTGCTGTAGGCGACGGACAACAGGCAGTGGATGCTCTAAATGGCATGCCTGGTGTTGAAAGAAACACAATAACATCTCAAACTGAGATTCTAAGTTTCCCTTCATATGACTTGATCCTAATGGATTGTCAGATGCCAAAGATGGATGGATATGAGGCAACAAAAGAAATAAGGAAATCAGAAATTGGAACTGCATTCCACATTCCAATCGTCGCTCTTACAGCTCATGCAATGTCATGTGATGAAGCCAAATGTTTAGAAGTGGGCATGGATGCTTATTTAACCAAGCCAATTGACTTTAAGCTGATGGAGTCTACCATCCTTTCACTCACAAAAAGAGAAACCTTGAAATCTTAA
Protein sequence

>MsaT044215.1

MATKCMHVFDRLLLCFATCWKKNSTLTGRRKFHKDVEKEEFQYSTTQCLSSYYSVFVVRLAIMVMLAILIGLLTILTWHFTKIYTTKSLNSLAYDLRYELLQRPILRMWNILNSTAEITTAQVKLSEYVIRSHGNLATQTEKVEMYESMRAVTWALFASRKALNSITVKYRNGFVQAFHRDLKDNNIFYIYTDLSYNETNSFAAYEDTHSNKSAIWYREQLDPVNGEKIGKAMKIAPEDSISIAGLSQVPDGVASWHVSVGKFTDSPLLSAALPVWDSSNKSIVAVVGVTTALYSVGQLMKELVDKHSGHMYLTSQEGYLLATSTNDPLLTNSTKKPELKMAVDCDNEVIREGAMWLKKTYGNNFPPSHEVHEENARLGHQQYYIDSFFLILKKLPLVGVIIIPRKHIMGEADERAFKTLVILISASLCIIVIGCVCILILTDGVSKEMNLRAELISHLEARRKAEASSNYKSQFLANMSHELRTPMAAVIGLLDILLSDDHLTNEQCATVTQIRKCSTALLHLLNNILDISKVESGKLVLEEAEFDFGRELEGLVDMFSVQCINHNVEIILDLSDDMPKLVRGDSARVVQIFANLINNSIKFTLSGHIILRGWCENPNSYGDSENSTLEQKQFGCSQKTKMKQHENHSKKTSNIDNKMILWFEVDDTGCGIDPSKWDSVFESFEQADLSTTRLHGGTGLGLCIVRSLVNKMGGEIKVVKKEGPGTLMRLYMRLTAPVDTTEQHCQVDFANNNLVVLLALHGNMSRLITSKWLQKNGVATMEASEWNGLTQILKELFRAKTSTHNNDFDAHFPAPEGLNSKFISIQELPNPTFVIVVDIDLLDLSTDIWKEQLNFLHKYYARAKFVWLQNHDSSNTVKTELRKKGHILTVNKPLYKAKMIHILDDVIKERNVEVQKKNMKEDDLHESLEIEYTHHCDVASSDGSDISEIGSSNLVTANGDKQREEVVRINPSSLYQKSNCLLGLSNGYMEHKEAFISTRAKGEDSKGSETSRISGSSKAMNGKKSLEGLRILLAEDTAVIQRVATIMLEKMGATVVAVGDGQQAVDALNGMPGVERNTITSQTEILSFPSYDLILMDCQMPKMDGYEATKEIRKSEIGTAFHIPIVALTAHAMSCDEAKCLEVGMDAYLTKPIDFKLMESTILSLTKRETLKS*