AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044226

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044226 MtrunA17_Chr8g0371801 96.014 276 7 4 1 273 1 275 1.11e-176 488
MsaG044226 MtrunA17_Chr4g0037381 56.410 78 34 0 1 78 1 78 7.99e-25 96.7
MsaG044226 MtrunA17_Chr3g0080991 36.686 169 67 4 105 270 1517 1648 1.05e-17 83.2
MsaG044226 MtrunA17_Chr3g0080991 37.647 170 67 4 104 270 1458 1591 2.16e-17 82.0
MsaG044226 MtrunA17_Chr3g0080991 38.235 170 64 4 105 270 1488 1620 2.60e-17 82.0
MsaG044226 MtrunA17_Chr3g0080991 40.223 179 73 5 104 268 2052 2210 3.61e-15 75.5
MsaG044226 MtrunA17_Chr3g0080991 37.436 195 81 5 87 270 1973 2137 2.63e-12 67.0
MsaG044226 MtrunA17_Chr5g0439981 28.144 167 80 7 105 268 354 483 3.44e-12 66.2
MsaG044226 MtrunA17_Chr3g0119021 23.466 277 151 7 22 269 67 311 2.56e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044226 AT4G36020.2 46.043 278 126 6 1 272 6 265 1.06e-56 183
MsaG044226 AT4G36020.1 43.234 303 132 6 1 272 6 299 1.27e-56 184
MsaG044226 AT4G36020.3 43.234 303 132 6 1 272 6 299 1.27e-56 184
MsaG044226 AT2G17870.1 45.455 297 128 6 5 272 10 301 2.57e-46 157
MsaG044226 AT2G21060.1 38.579 197 98 3 4 189 13 197 4.80e-16 75.1
MsaG044226 AT3G42860.1 31.387 137 72 3 158 272 223 359 2.47e-15 75.5
MsaG044226 AT3G42860.1 33.333 153 78 5 79 217 217 359 4.46e-15 74.7
MsaG044226 AT3G43590.1 23.111 225 100 4 90 273 153 345 2.07e-12 67.4

Find 105 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACGGTGGCGGTGGATA+TGG 0.297469 8:-57465022 MsaT044226.1:CDS
AGGGATTGTGTTGAGGCTTC+TGG 0.315257 8:-57464710 MsaT044226.1:CDS
TCCTAAGGGTGAACCTCTTC+AGG 0.319731 8:-57465293 MsaT044226.1:CDS
CCGCCACCGCCTCCGCTACT+AGG 0.384433 8:+57464938 None:intergenic
ACCGGTGACATCAACGGCTT+TGG 0.389735 8:+57465315 None:intergenic
TATGGTGGAGGCGGCGGTCG+TGG 0.391127 8:-57465256 MsaT044226.1:CDS
AGAAGGTTTGGCGGTGGTGG+TGG 0.393791 8:-57464791 MsaT044226.1:CDS
TGTTATACTTGTGGTGATAC+TGG 0.397825 8:-57465193 MsaT044226.1:CDS
GTGTTGAGGCTTCTGGTTGA+TGG 0.398672 8:-57464703 None:intergenic
AACAGAAGGTTTGGCGGTGG+TGG 0.399361 8:-57464794 MsaT044226.1:CDS
TTTGATGAATCCGAAGCCTT+TGG 0.409445 8:+57465459 None:intergenic
GGTGGTGGCAACAGAAGGTT+TGG 0.419148 8:-57464803 MsaT044226.1:CDS
GGCGGCGGTCGTGGATTTAG+AGG 0.428537 8:-57465247 MsaT044226.1:CDS
TGCTACAGGTGTGGTGGGGT+TGG 0.430495 8:-57464986 MsaT044226.1:CDS
CGTTGATGTCACCGGTCCTA+AGG 0.435362 8:-57465308 MsaT044226.1:CDS
TTCAACAACACCAAAGGCTT+CGG 0.440293 8:-57465469 MsaT044226.1:CDS
AGGTTTGGCGGTGGTGGTGG+CGG 0.444094 8:-57464788 MsaT044226.1:CDS
AGAAGGAACGGTGGTGGTGG+TGG 0.446028 8:-57465217 MsaT044226.1:CDS
CATCTTGCTACAGGTGTGGT+GGG 0.457817 8:-57464991 MsaT044226.1:CDS
TGCTTTAACTGTGGGAAGGC+AGG 0.462177 8:-57464743 MsaT044226.1:CDS
GGCGGTCGCGATGGAGGAAA+AGG 0.463269 8:-57464770 MsaT044226.1:CDS
AGTAGCGGAGGCGGTGGCGG+CGG 0.469312 8:-57464935 MsaT044226.1:CDS
GAGAGAAGGAACGGTGGTGG+TGG 0.470011 8:-57465220 MsaT044226.1:CDS
GAGATCTTCGCTATCGTCAT+CGG 0.473073 8:+57465435 None:intergenic
GATCGCTCTGGTGGTGGTGG+TGG 0.473309 8:-57465124 MsaT044226.1:CDS
GATGGCAATGGAAGGTTTGG+TGG 0.475514 8:-57464821 MsaT044226.1:CDS
AGATCTTCGCTATCGTCATC+GGG 0.477231 8:+57465436 None:intergenic
CCTAGTAGCGGAGGCGGTGG+CGG 0.478415 8:-57464938 MsaT044226.1:CDS
AACAACAACAACGGTGGCGG+TGG 0.478614 8:-57465028 MsaT044226.1:CDS
GTGAGGTGGGTCACATAGCT+AGG 0.482831 8:-57464895 MsaT044226.1:CDS
GATGATGGCAATGGAAGGTT+TGG 0.485258 8:-57464824 MsaT044226.1:CDS
TTGCTATAAGTGCGGTGAGG+TGG 0.492734 8:-57464909 MsaT044226.1:CDS
ACCTGAAGAGGTTCACCCTT+AGG 0.495108 8:+57465292 None:intergenic
GCTAGGGATTGCAGCAATGA+AGG 0.497533 8:-57464878 MsaT044226.1:CDS
GTGATACTGGTCACATCGCT+AGG 0.498797 8:-57465180 MsaT044226.1:CDS
GATAACTATGGTGGAGGCGG+CGG 0.501060 8:-57465262 MsaT044226.1:CDS
ACATCTTGCTACAGGTGTGG+TGG 0.503337 8:-57464992 MsaT044226.1:CDS
GGTGGTGGCGGTCGCGATGG+AGG 0.503410 8:-57464776 MsaT044226.1:CDS
ACAATCGCCGACGGTGATAA+CGG 0.505414 8:-57465343 MsaT044226.1:CDS
AGGGTGAACCTCTTCAGGTT+AGG 0.518271 8:-57465288 MsaT044226.1:CDS
GTGGAGGCACATCTTGCTAC+AGG 0.518304 8:-57465000 MsaT044226.1:CDS
TGCTATAAGTGCGGTGAGGT+GGG 0.518807 8:-57464908 MsaT044226.1:CDS
ATCGTTCCGATCGGAATGAT+AGG 0.521685 8:-57465150 MsaT044226.1:CDS
GGTGGTGGTGGCGGTCGCGA+TGG 0.528129 8:-57464779 MsaT044226.1:CDS
TGAACCTCTTCAGGTTAGGC+AGG 0.528588 8:-57465284 MsaT044226.1:CDS
GGTGAGAGAAGGAACGGTGG+TGG 0.532764 8:-57465223 MsaT044226.1:CDS
AATGATCGCTCTGGTGGTGG+TGG 0.533030 8:-57465127 MsaT044226.1:CDS
GGTGGCAACAGAAGGTTTGG+CGG 0.535347 8:-57464800 MsaT044226.1:CDS
TTTGCTAGGGATTGCATGCG+TGG 0.536617 8:-57465058 MsaT044226.1:CDS
CAGGTTAGGCAGGATAACTA+TGG 0.541696 8:-57465274 MsaT044226.1:CDS
CAATCTGACATCAGATCTGA+AGG 0.544978 8:-57465403 MsaT044226.1:CDS
GAATCAGCGGCGTGGTGCAA+TGG 0.549203 8:-57465492 MsaT044226.1:CDS
ACCAAAGCCGTTGATGTCAC+CGG 0.550585 8:-57465316 MsaT044226.1:CDS
GACCGTGCTTGCTATACTTG+TGG 0.551573 8:-57465094 MsaT044226.1:CDS
CAGGATAACTATGGTGGAGG+CGG 0.552223 8:-57465265 MsaT044226.1:CDS
TTATCCTGCCTAACCTGAAG+AGG 0.553752 8:+57465280 None:intergenic
GGTTTGGTGGTGGCAACAGA+AGG 0.556716 8:-57464808 MsaT044226.1:CDS
AACGGTGGCGGTGGATATGG+TGG 0.558225 8:-57465019 MsaT044226.1:CDS
AATGAAGGAGGAAGGTATGA+TGG 0.561449 8:-57464863 MsaT044226.1:CDS
AGAGGTGGTGAGAGAAGGAA+CGG 0.569558 8:-57465229 MsaT044226.1:CDS
GATTTAGAGGTGGTGAGAGA+AGG 0.570831 8:-57465234 MsaT044226.1:CDS
AAAGGCACATGCTTTAACTG+TGG 0.571520 8:-57464752 MsaT044226.1:CDS
GGTGGTGGTTGTTATACTTG+TGG 0.572889 8:-57465202 MsaT044226.1:CDS
GGCAACAGAAGGTTTGGCGG+TGG 0.573285 8:-57464797 MsaT044226.1:CDS
AGGAATGATCGCTCTGGTGG+TGG 0.574103 8:-57465130 MsaT044226.1:CDS
AAGGCACATGCTTTAACTGT+GGG 0.579793 8:-57464751 MsaT044226.1:CDS
AATGATAGGAATGATCGCTC+TGG 0.580640 8:-57465136 MsaT044226.1:CDS
GTTGATGTCACCGGTCCTAA+GGG 0.586474 8:-57465307 MsaT044226.1:CDS
AAGAGGTTCACCCTTAGGAC+CGG 0.590248 8:+57465297 None:intergenic
GGTATGATGGTGGCAATGCA+AGG 0.590765 8:-57464850 MsaT044226.1:CDS
GGCAATGGAAGGTTTGGTGG+TGG 0.602525 8:-57464818 MsaT044226.1:CDS
ACTCCTAGTAGCGGAGGCGG+TGG 0.603004 8:-57464941 MsaT044226.1:CDS
AACCACAAGTATAGCAAGCA+CGG 0.605269 8:+57465092 None:intergenic
ATCTTGCTACAGGTGTGGTG+GGG 0.605984 8:-57464990 MsaT044226.1:CDS
CTTAGGACCGGTGACATCAA+CGG 0.607581 8:+57465309 None:intergenic
GGTGGCGGTGGATATGGTGG+AGG 0.611131 8:-57465016 MsaT044226.1:CDS
ATCATTCCTATCATTCCGAT+CGG 0.613940 8:+57465144 None:intergenic
AAGTATAGCAAGCACGGTCA+CGG 0.614873 8:+57465098 None:intergenic
TGAGGTGGGTCACATAGCTA+GGG 0.619943 8:-57464894 MsaT044226.1:CDS
GTCGAGTTCACAATCGCCGA+CGG 0.621401 8:-57465352 MsaT044226.1:CDS
GGCAATGCAAGGTATGATGA+TGG 0.625626 8:-57464839 MsaT044226.1:CDS
CACATGCTTTAACTGTGGGA+AGG 0.629671 8:-57464747 MsaT044226.1:CDS
GTTAGGCAGGATAACTATGG+TGG 0.630912 8:-57465271 MsaT044226.1:CDS
AGGCAGGATAACTATGGTGG+AGG 0.633369 8:-57465268 MsaT044226.1:CDS
GGTATGATGATGGCAATGGA+AGG 0.633617 8:-57464829 MsaT044226.1:CDS
GGCGGTCGTGGATTTAGAGG+TGG 0.635145 8:-57465244 MsaT044226.1:CDS
AGGGATTGCAGCAATGAAGG+AGG 0.636247 8:-57464875 MsaT044226.1:CDS
GGCACATCTTGCTACAGGTG+TGG 0.637876 8:-57464995 MsaT044226.1:CDS
ATTGCAGCAATGAAGGAGGA+AGG 0.639616 8:-57464871 MsaT044226.1:CDS
CAATGGTTCAACAACACCAA+AGG 0.639643 8:-57465475 MsaT044226.1:CDS
GCAAGGTATGATGATGGCAA+TGG 0.642927 8:-57464833 MsaT044226.1:CDS
TGATACTGGTCACATCGCTA+GGG 0.645119 8:-57465179 MsaT044226.1:CDS
GGGATTGTGATCGTTCCGAT+CGG 0.645526 8:-57465159 MsaT044226.1:CDS
GCTACTCCTAGTAGCGGAGG+CGG 0.647305 8:-57464944 MsaT044226.1:CDS
TGCGCTACTCCTAGTAGCGG+AGG 0.648929 8:-57464947 MsaT044226.1:CDS
GCTAGGGATTGCATGCGTGG+AGG 0.656728 8:-57465055 MsaT044226.1:CDS
GGTGGTGAGAGAAGGAACGG+TGG 0.661941 8:-57465226 MsaT044226.1:CDS
GGCGGTGCTTGCTATAAGTG+CGG 0.662649 8:-57464917 MsaT044226.1:CDS
GATTGCGCTACTCCTAGTAG+CGG 0.671173 8:-57464950 MsaT044226.1:CDS
GATAGGAATGATCGCTCTGG+TGG 0.671256 8:-57465133 MsaT044226.1:CDS
GGAGGAGAGAATCAGCGGCG+TGG 0.687679 8:-57465500 MsaT044226.1:CDS
GAAGGAGGAAGGTATGATGG+TGG 0.690298 8:-57464860 MsaT044226.1:CDS
ATGGCGGAGGAGAGAATCAG+CGG 0.694395 8:-57465505 MsaT044226.1:CDS
GGAGGTAACAACAACAACAA+CGG 0.704702 8:-57465037 MsaT044226.1:CDS
TGCTTGCTATAAGTGCGGTG+AGG 0.738293 8:-57464912 MsaT044226.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 57464706 57465527 57464706 ID=MsaG044226
Chr8 mRNA 57464706 57465527 57464706 ID=MsaT044226.1;Parent=MsaG044226
Chr8 exon 57464706 57465527 57464706 ID=MsaT044226.1.exon1;Parent=MsaT044226.1
Chr8 CDS 57464706 57465527 57464706 ID=cds.MsaT044226.1;Parent=MsaT044226.1
Gene Sequence

>MsaT044226.1

ATGGCGGAGGAGAGAATCAGCGGCGTGGTGCAATGGTTCAACAACACCAAAGGCTTCGGATTCATCAAACCCGATGACGATAGCGAAGATCTCTTCGTTCATCAATCTGACATCAGATCTGAAGGATTTCGCTCTCTTTTCGAAGGCGATCGCGTCGAGTTCACAATCGCCGACGGTGATAACGGTAAAACCAAAGCCGTTGATGTCACCGGTCCTAAGGGTGAACCTCTTCAGGTTAGGCAGGATAACTATGGTGGAGGCGGCGGTCGTGGATTTAGAGGTGGTGAGAGAAGGAACGGTGGTGGTGGTGGTTGTTATACTTGTGGTGATACTGGTCACATCGCTAGGGATTGTGATCGTTCCGATCGGAATGATAGGAATGATCGCTCTGGTGGTGGTGGTGGAGACCGTGACCGTGCTTGCTATACTTGTGGTTCTTTTGAACATTTTGCTAGGGATTGCATGCGTGGAGGTAACAACAACAACAACGGTGGCGGTGGATATGGTGGAGGCACATCTTGCTACAGGTGTGGTGGGGTTGGTCACATAGCAAGAGATTGCGCTACTCCTAGTAGCGGAGGCGGTGGCGGCGGTGCTTGCTATAAGTGCGGTGAGGTGGGTCACATAGCTAGGGATTGCAGCAATGAAGGAGGAAGGTATGATGGTGGCAATGCAAGGTATGATGATGGCAATGGAAGGTTTGGTGGTGGCAACAGAAGGTTTGGCGGTGGTGGTGGCGGTCGCGATGGAGGAAAAGGCACATGCTTTAACTGTGGGAAGGCAGGACATTTTGCAAGGGATTGTGTTGAGGCTTCTGGTTGA
Protein sequence

>MsaT044226.1

MAEERISGVVQWFNNTKGFGFIKPDDDSEDLFVHQSDIRSEGFRSLFEGDRVEFTIADGDNGKTKAVDVTGPKGEPLQVRQDNYGGGGGRGFRGGERRNGGGGGCYTCGDTGHIARDCDRSDRNDRNDRSGGGGGDRDRACYTCGSFEHFARDCMRGGNNNNNGGGGYGGGTSCYRCGGVGHIARDCATPSSGGGGGGACYKCGEVGHIARDCSNEGGRYDGGNARYDDGNGRFGGGNRRFGGGGGGRDGGKGTCFNCGKAGHFARDCVEASG*